American Journal of Physiology. Lung Cellular and Molecular Physiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Low volume ventilation of pre-injured lungs degrades lung function via stress concentration and progressive alveolar collapse
Am J Physiol Lung Cell Mol Physiol. 2024 May 7. doi: 10.1152/ajplung.00323.2023. Online ahead of print.ABSTRACTMechanical ventilation can cause ventilation-induced lung injury (VILI). The concept of stress concentrations suggests that surfactant dysfunction-induced microatelectases might impose injurious stresses on adjacent, open alveoli and function as germinal centers for injury propagation. The aim of the present study was to quantify the histopathological pattern of VILI progression and to test the hypothesis that injury progresses at the interface between microatelectases and ventilated lung parenchyma during low pos...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Richard Zimmermann Franziska Roeder Clemens Ruppert Bradford J Smith Lars Knudsen Source Type: research

The fix is not yet in: recommendation for fixation of lungs within physiologic/pathophysiologic volume range in preclinical pulmonary structure-function studies
Am J Physiol Lung Cell Mol Physiol. 2024 May 7. doi: 10.1152/ajplung.00341.2023. Online ahead of print.ABSTRACTQuantitative characterization of lung structures by morphometric or stereologic analysis of histologic sections is a powerful means of elucidating pulmonary structure-function relations. The overwhelming majority of studies, however, fix lungs for histology at pressures outside the physiologic/pathophysiologic respiratory volume range. Thus valuable information is being lost. In this perspective article, we argue that investigators performing pulmonary histologic studies should consider whether the aims of their s...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Carrie E Perlman Lars Knudsen Bradford J Smith Source Type: research

Antenatal creatine supplementation reduces persistent fetal lung inflammation and oxidative stress in an ovine model of chorioamnionitis
Conclusion Fetal creatine supplementation reduced markers of inflammation and oxidative stress in the fetal lungs arising from chorioamnionitis.PMID:38712443 | DOI:10.1152/ajplung.00241.2023 (Source: American Journal of Physiology. Lung Cellular and Molecular Physiology)
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Y Jane Choi Ellen Williams Mar Janna Dahl Sebastian E Amos Christopher James Angelo P Bautista Veena Kurup Gabrielle C Musk Helen Kershaw Peter G Arthur Anthony Kicic Yu Suk Choi Jessica R Terrill J Jane Pillow Source Type: research

Elastin-derived peptides favor type 2 innate lymphoid cells in COPD
This study aimed to investigate the presence of ILC in COPD patients and examine the impact of elastin peptides on their functionality. Our findings revealed an elevated proportion of ILC2 in the peripheral blood of COPD patients, and a general activation of ILC as indicated by an increase in their cytokine secretion capacity. Notably, our study demonstrated that serum from COPD patients promotes ILC2 phenotype, likely due to the elevated concentration of IL-5, a cytokine known to favor ILC2 activation. Furthermore, we uncovered that this increase in IL-5 secretion is partially attributed to its secretion by macrophages up...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Sarah Lahire Caroline Fichel Oc éane Rubaszewski C édric Lereverend Sandra Audonnet Vincent Visneux Jeanne-Marie Perotin Ga ëtan Deslée S ébastien Le Jan St éphane Potteaux Richard Le Naour Arnaud Pommier Source Type: research

Effect of Estrogen Receptor Alpha on Cardiopulmonary Adaptation to Chronic Developmental Hypoxia in a Rat Model
Am J Physiol Lung Cell Mol Physiol. 2024 May 7. doi: 10.1152/ajplung.00161.2023. Online ahead of print.ABSTRACTHumans living at high-altitude (HA) have adapted to this environment by increasing pulmonary vascular and alveolar growth. RNA sequencing data from a novel murine model that mimics this phenotypical response to HA suggested estrogen signaling via estrogen receptor alpha (ERα) may be involved in this adaptation. We hypothesized ERα was a key mediator in the cardiopulmonary adaption to chronic hypoxia and sought to delineate the mechanistic role ERα contributes to this process by exposing novel loss-of-function E...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Nicholas T Severyn Patricia Esparza Huanling Gao Elizabeth A Mickler Marjorie E Albrecht Amanda J Fisher Bakhtiyor Yakubov Todd G Cook James E Slaven Avram D Walts Robert S Tepper Tim Lahm Source Type: research

NFkB/RelA Signaling in Secretoglobin Progenitors Mediates Plasticity and MMP-Induced Barrier Disruption In House Dust Mite-Induced Allergic Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 May 7. doi: 10.1152/ajplung.00066.2024. Online ahead of print.ABSTRACTThe house dust mite (HDM) represents a major cause of allergic rhinitis and asthma. We tested whether HDM-induced aeroallergen exposure sensitivity is caused by the innate-immune response in small airway epithelial cells. HDM exposure rapidly activates NFkB/RelA in the Secretoglobin (Scgb1a1+) lineage and upregulates markers of epithelial plasticity. To determine the effect of epithelial NFkB signaling, NFkB was depleted in a tamoxifen (TMX)-inducible Scgb1a1-CreERTM mouse within a CL57B/L6 background. Corn oil or...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Melissa E Skibba Allan R Brasier Source Type: research

< em > Good Things < /em > : Announcing the next round of the American Journal of Physiology Lung Cellular and Molecular Physiology Editorial Board Fellows Program
Am J Physiol Lung Cell Mol Physiol. 2024 May 7. doi: 10.1152/ajplung.00133.2024. Online ahead of print.NO ABSTRACTPMID:38713757 | DOI:10.1152/ajplung.00133.2024 (Source: American Journal of Physiology. Lung Cellular and Molecular Physiology)
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 7, 2024 Category: Cytology Authors: Larissa A Shimoda Cristina M Alvira Julie A Bastarache Rodney D Britt Wolfgang M Kuebler Thiago S Moreira Eric P Schmidt Source Type: research

Hypoxic perfusion of pulmonary arterial vasa vasorum increases pulmonary arterial pressure
In conclusion, the results of the current study prove that hypoxic perfusion of the vasa vasorum of the PA directly increases PAP in an ex situ lung perfusion setup suggesting that PA vasa vasorum function and wall ischemia may contribute to the development of PH.PMID:38651234 | DOI:10.1152/ajplung.00346.2023 (Source: American Journal of Physiology. Lung Cellular and Molecular Physiology)
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Emma L Heise Jawad Salman Karolin S Webs Klaus H öffler Christina Brandenberger Dietmar B öthig Christian M ühlfeld Axel Haverich Source Type: research

A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research

Aberrant Long-Chain Fatty Acid Metabolism Associated with Evolving Systemic Sclerosis-Associated Pulmonary Arterial Hypertension
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00057.2024. Online ahead of print.ABSTRACTWe sought to investigate differential metabolism in patients with systemic sclerosis (SSc) who develop pulmonary arterial hypertension (PAH) versus those who do not, as a method of identifying potential disease biomarkers. In a nested case-control design, serum metabolites were assayed in SSc subjects who developed right heart catheterization-confirmed PAH (n=22) while under surveillance in a longitudinal cohort from Johns Hopkins, then compared to metabolites assayed in matched SSc patients who did not develop P...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Julie C Coursen Tijana Tuhy Mario Naranjo Adrianne Woods Laura K Hummers Ami A Shah Karthik Suresh Scott H Visovatti Stephen C Mathai Paul M Hassoun Rachel L Damico Catherine E Simpson Source Type: research

A mouse model of progressive lung fibrosis with cutaneous involvement induced by a combination of oropharyngeal and osmotic minipump bleomycin delivery
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00408.2023. Online ahead of print.ABSTRACTSystemic sclerosis (SSc) with interstitial lung disease (SSc-ILD) lacks curative pharmacological treatments, thus necessitating effective animal models for candidate drug discovery. Existing Bleomycin (BLM)-induced SSc-ILD mouse models feature spatially limited pulmonary fibrosis, spontaneously resolving after 28 days. Here, we present an alternative BLM administration approach in female C57BL/6 mice, combining oropharyngeal aspiration (OA) and subcutaneous mini-pump delivery (pump) of BLM to induce a sustained a...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Andrea Grandi Erica Ferrini Matteo Zoboli Davide Buseghin Francesca Pennati Zahra Khalajzeyqami Roberta Ciccimarra Gino Villetti Franco Fabio Stellari Source Type: research

Progesterone amplifies allergic inflammation and airway pathology in association with higher lung ILC2 responses
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00207.2023. Online ahead of print.ABSTRACTPerimenstrual worsening of asthma occurs in up to 40% of asthmatic women, leading to increased acute exacerbations requiring clinical care. The role of sex hormones during these times remains unclear. In the current study, we used a translational approach to determine whether progesterone exacerbates allergic inflammation in the traditional OVA model in BALB/c mice. Simultaneously, we used PBMC from healthy human donors to assess the effects of progesterone on circulating ILC2. Briefly, lungs of ovarectomized (OV...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Shubhanshi Trivedi Cassandra E Deering-Rice Samuel E Aamodt Thomas P Huecksteadt Elizabeth J Myers Karl A Sanders Robert Paine Kristi J Warren Source Type: research

Hypoxic perfusion of pulmonary arterial vasa vasorum increases pulmonary arterial pressure
In conclusion, the results of the current study prove that hypoxic perfusion of the vasa vasorum of the PA directly increases PAP in an ex situ lung perfusion setup suggesting that PA vasa vasorum function and wall ischemia may contribute to the development of PH.PMID:38651234 | DOI:10.1152/ajplung.00346.2023 (Source: American Journal of Physiology. Lung Cellular and Molecular Physiology)
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Emma L Heise Jawad Salman Karolin S Webs Klaus H öffler Christina Brandenberger Dietmar B öthig Christian M ühlfeld Axel Haverich Source Type: research

A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research

Aberrant Long-Chain Fatty Acid Metabolism Associated with Evolving Systemic Sclerosis-Associated Pulmonary Arterial Hypertension
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00057.2024. Online ahead of print.ABSTRACTWe sought to investigate differential metabolism in patients with systemic sclerosis (SSc) who develop pulmonary arterial hypertension (PAH) versus those who do not, as a method of identifying potential disease biomarkers. In a nested case-control design, serum metabolites were assayed in SSc subjects who developed right heart catheterization-confirmed PAH (n=22) while under surveillance in a longitudinal cohort from Johns Hopkins, then compared to metabolites assayed in matched SSc patients who did not develop P...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Julie C Coursen Tijana Tuhy Mario Naranjo Adrianne Woods Laura K Hummers Ami A Shah Karthik Suresh Scott H Visovatti Stephen C Mathai Paul M Hassoun Rachel L Damico Catherine E Simpson Source Type: research