Airway and parenchymal tissue resistance and elastance in ex vivo sheep lungs: Effects of bronchochallenge and deep inspiration
Am J Physiol Lung Cell Mol Physiol. 2022 May 10. doi: 10.1152/ajplung.00033.2022. Online ahead of print.ABSTRACTLung resistance (RL) is determined by airway and parenchymal tissue resistance, as well as the degree of heterogeneity in airway constriction. Deep inspirations (DIs) are known to reverse experimentally induced increase in RL, but the mechanism is not entirely clear. The first step towards understanding the effect of DI is to determine how each of the resistance components is affected by DI. In the present study, we measured RL and apparent airway resistance (RAW, which combines the effects of airway resistance a...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Shou-Jin Dong Lu Wang Pasquale Chitano Dragos Mihai Vasilescu Peter D Par é Chun Y Seow Source Type: research

Cellular mechanism underlying the facilitation of contractile response induced by IL-25 in mouse tracheal smooth muscle
This study aims to investigate the effects of IL-25 on mouse tracheal smooth muscle contractility and elucidate the cellular mechanisms. Incubation with IL-25 augmented the contraction of mouse tracheal smooth muscles, which could be suppressed by the L-type voltage-dependent Ca2+ channel (L-VDCC) blocker nifedipine. Furthermore, IL-25 enhanced the cytosolic Ca2+ signals and triggered up-regulation of α1C L-VDCC (CaV1.2) in primary cultured mouse tracheal smooth muscle cells. Knocking down IL-17RA/IL-17RB receptors or inhibiting the transforming growth factor-β-activated kinase 1 (TAK1)-tumor progression locus 2 (TPL2)-M...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Ze-Xin Huang Zhuo-Er Qiu Lei Chen Xiao-Chun Hou Yun-Xin Zhu Wen-Liang Zhou Yi-Lin Zhang Source Type: research

Airway and parenchymal tissue resistance and elastance in ex vivo sheep lungs: Effects of bronchochallenge and deep inspiration
Am J Physiol Lung Cell Mol Physiol. 2022 May 10. doi: 10.1152/ajplung.00033.2022. Online ahead of print.ABSTRACTLung resistance (RL) is determined by airway and parenchymal tissue resistance, as well as the degree of heterogeneity in airway constriction. Deep inspirations (DIs) are known to reverse experimentally induced increase in RL, but the mechanism is not entirely clear. The first step towards understanding the effect of DI is to determine how each of the resistance components is affected by DI. In the present study, we measured RL and apparent airway resistance (RAW, which combines the effects of airway resistance a...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Shou-Jin Dong Lu Wang Pasquale Chitano Dragos Mihai Vasilescu Peter D Par é Chun Y Seow Source Type: research

Cellular mechanism underlying the facilitation of contractile response induced by IL-25 in mouse tracheal smooth muscle
This study aims to investigate the effects of IL-25 on mouse tracheal smooth muscle contractility and elucidate the cellular mechanisms. Incubation with IL-25 augmented the contraction of mouse tracheal smooth muscles, which could be suppressed by the L-type voltage-dependent Ca2+ channel (L-VDCC) blocker nifedipine. Furthermore, IL-25 enhanced the cytosolic Ca2+ signals and triggered up-regulation of α1C L-VDCC (CaV1.2) in primary cultured mouse tracheal smooth muscle cells. Knocking down IL-17RA/IL-17RB receptors or inhibiting the transforming growth factor-β-activated kinase 1 (TAK1)-tumor progression locus 2 (TPL2)-M...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Ze-Xin Huang Zhuo-Er Qiu Lei Chen Xiao-Chun Hou Yun-Xin Zhu Wen-Liang Zhou Yi-Lin Zhang Source Type: research

Airway and parenchymal tissue resistance and elastance in ex vivo sheep lungs: Effects of bronchochallenge and deep inspiration
Am J Physiol Lung Cell Mol Physiol. 2022 May 10. doi: 10.1152/ajplung.00033.2022. Online ahead of print.ABSTRACTLung resistance (RL) is determined by airway and parenchymal tissue resistance, as well as the degree of heterogeneity in airway constriction. Deep inspirations (DIs) are known to reverse experimentally induced increase in RL, but the mechanism is not entirely clear. The first step towards understanding the effect of DI is to determine how each of the resistance components is affected by DI. In the present study, we measured RL and apparent airway resistance (RAW, which combines the effects of airway resistance a...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Shou-Jin Dong Lu Wang Pasquale Chitano Dragos Mihai Vasilescu Peter D Par é Chun Y Seow Source Type: research

Cellular mechanism underlying the facilitation of contractile response induced by IL-25 in mouse tracheal smooth muscle
This study aims to investigate the effects of IL-25 on mouse tracheal smooth muscle contractility and elucidate the cellular mechanisms. Incubation with IL-25 augmented the contraction of mouse tracheal smooth muscles, which could be suppressed by the L-type voltage-dependent Ca2+ channel (L-VDCC) blocker nifedipine. Furthermore, IL-25 enhanced the cytosolic Ca2+ signals and triggered up-regulation of α1C L-VDCC (CaV1.2) in primary cultured mouse tracheal smooth muscle cells. Knocking down IL-17RA/IL-17RB receptors or inhibiting the transforming growth factor-β-activated kinase 1 (TAK1)-tumor progression locus 2 (TPL2)-M...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Ze-Xin Huang Zhuo-Er Qiu Lei Chen Xiao-Chun Hou Yun-Xin Zhu Wen-Liang Zhou Yi-Lin Zhang Source Type: research

Airway and parenchymal tissue resistance and elastance in ex vivo sheep lungs: Effects of bronchochallenge and deep inspiration
Am J Physiol Lung Cell Mol Physiol. 2022 May 10. doi: 10.1152/ajplung.00033.2022. Online ahead of print.ABSTRACTLung resistance (RL) is determined by airway and parenchymal tissue resistance, as well as the degree of heterogeneity in airway constriction. Deep inspirations (DIs) are known to reverse experimentally induced increase in RL, but the mechanism is not entirely clear. The first step towards understanding the effect of DI is to determine how each of the resistance components is affected by DI. In the present study, we measured RL and apparent airway resistance (RAW, which combines the effects of airway resistance a...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Shou-Jin Dong Lu Wang Pasquale Chitano Dragos Mihai Vasilescu Peter D Par é Chun Y Seow Source Type: research

Cellular mechanism underlying the facilitation of contractile response induced by IL-25 in mouse tracheal smooth muscle
This study aims to investigate the effects of IL-25 on mouse tracheal smooth muscle contractility and elucidate the cellular mechanisms. Incubation with IL-25 augmented the contraction of mouse tracheal smooth muscles, which could be suppressed by the L-type voltage-dependent Ca2+ channel (L-VDCC) blocker nifedipine. Furthermore, IL-25 enhanced the cytosolic Ca2+ signals and triggered up-regulation of α1C L-VDCC (CaV1.2) in primary cultured mouse tracheal smooth muscle cells. Knocking down IL-17RA/IL-17RB receptors or inhibiting the transforming growth factor-β-activated kinase 1 (TAK1)-tumor progression locus 2 (TPL2)-M...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Ze-Xin Huang Zhuo-Er Qiu Lei Chen Xiao-Chun Hou Yun-Xin Zhu Wen-Liang Zhou Yi-Lin Zhang Source Type: research

Airway and parenchymal tissue resistance and elastance in ex vivo sheep lungs: Effects of bronchochallenge and deep inspiration
Am J Physiol Lung Cell Mol Physiol. 2022 May 10. doi: 10.1152/ajplung.00033.2022. Online ahead of print.ABSTRACTLung resistance (RL) is determined by airway and parenchymal tissue resistance, as well as the degree of heterogeneity in airway constriction. Deep inspirations (DIs) are known to reverse experimentally induced increase in RL, but the mechanism is not entirely clear. The first step towards understanding the effect of DI is to determine how each of the resistance components is affected by DI. In the present study, we measured RL and apparent airway resistance (RAW, which combines the effects of airway resistance a...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Shou-Jin Dong Lu Wang Pasquale Chitano Dragos Mihai Vasilescu Peter D Par é Chun Y Seow Source Type: research

Cellular mechanism underlying the facilitation of contractile response induced by IL-25 in mouse tracheal smooth muscle
This study aims to investigate the effects of IL-25 on mouse tracheal smooth muscle contractility and elucidate the cellular mechanisms. Incubation with IL-25 augmented the contraction of mouse tracheal smooth muscles, which could be suppressed by the L-type voltage-dependent Ca2+ channel (L-VDCC) blocker nifedipine. Furthermore, IL-25 enhanced the cytosolic Ca2+ signals and triggered up-regulation of α1C L-VDCC (CaV1.2) in primary cultured mouse tracheal smooth muscle cells. Knocking down IL-17RA/IL-17RB receptors or inhibiting the transforming growth factor-β-activated kinase 1 (TAK1)-tumor progression locus 2 (TPL2)-M...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 10, 2022 Category: Cytology Authors: Ze-Xin Huang Zhuo-Er Qiu Lei Chen Xiao-Chun Hou Yun-Xin Zhu Wen-Liang Zhou Yi-Lin Zhang Source Type: research

Racing Against Time: Leveraging Preclinical Models to Understand Pulmonary Susceptibility to Perinatal Acetaminophen Exposures
Am J Physiol Lung Cell Mol Physiol. 2022 May 3. doi: 10.1152/ajplung.00080.2022. Online ahead of print.ABSTRACTOver the past decade, clinicians have increasingly prescribed acetaminophen (APAP) for patients in the neonatal intensive care unit (NICU). Acetaminophen has been shown to reduce post-operative opiate burden, and may provide similar efficacy for closure of the patent ductus arteriosus (PDA) as nonsteroidal anti-inflammatory drugs (NSAIDs). Despite these potential benefits, APAP exposures have spread to increasingly less mature infants, a highly vulnerable population for whom robust pharmacokinetic and pharmacodyna...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 3, 2022 Category: Cytology Authors: David J McCulley Erik A Jensen Jennifer M S Sucre Sarah McKenna Laura G Sherlock Evgenia Dobrinskikh Clyde J Wright Source Type: research

The impact of nebulized Epoprostenol and Iloprost on hemoglobin oxygen affinity - an ex-vivo experiment
Conclusion Prostacyclin-receptor stimulation and subsequent cAMP mediated ATP release from erythrocytes might explain the effect of epoprostenol on Hb-O2 affinity but the reason for the sex hormone modified iloprost effect remains unclear. This finding is of great interest, as contraception is recommended for women taking iloprost. Being aware of potentially differing effects on Hb-O2 affinity might help select the right prostacyclin (epoprostenol vs. iloprost) depending on the patient and the underlying disease (e.g., acute respiratory distress syndrome vs. peripheral arterial disease).PMID:35503651 | DOI:10.1152/ajplung....
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 3, 2022 Category: Cytology Authors: Simon Woyke Norbert Mair Thomas Haller Marco Ronzani David Plunser Herbert Oberacher Hannes Gatterer Christopher Rugg Mathias Str öhle Source Type: research

Cortactin Loss Protects Against Hemin-Induced Acute Lung Injury in Sickle Cell Disease
Am J Physiol Lung Cell Mol Physiol. 2022 May 3. doi: 10.1152/ajplung.00274.2021. Online ahead of print.ABSTRACTIn patients with sickle cell disease (SCD), acute chest syndrome (ACS) is a major cause of morbidity and mortality. The pathophysiology of ACS is complex and hemin, the prosthetic moiety of hemoglobin, has been implicated in endothelial cell (EC) activation and subsequent ALI and ACS in vitro and in animal studies. Here, we examined the role of cortactin (CTTN), a cytoskeletal protein that regulates EC function, in response to hemin-induced ALI and ACS. Cortactin heterozygous (Cttn+/-) mice and their wild type sib...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 3, 2022 Category: Cytology Authors: Nicole M Jones Justin R Sysol Sunit Singla Patricia Smith George E Sandusky Huashan Wang Viswanathan Natarajan Steven M Dudek Roberto F Machado Source Type: research

Racing Against Time: Leveraging Preclinical Models to Understand Pulmonary Susceptibility to Perinatal Acetaminophen Exposures
Am J Physiol Lung Cell Mol Physiol. 2022 May 3. doi: 10.1152/ajplung.00080.2022. Online ahead of print.ABSTRACTOver the past decade, clinicians have increasingly prescribed acetaminophen (APAP) for patients in the neonatal intensive care unit (NICU). Acetaminophen has been shown to reduce post-operative opiate burden, and may provide similar efficacy for closure of the patent ductus arteriosus (PDA) as nonsteroidal anti-inflammatory drugs (NSAIDs). Despite these potential benefits, APAP exposures have spread to increasingly less mature infants, a highly vulnerable population for whom robust pharmacokinetic and pharmacodyna...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 3, 2022 Category: Cytology Authors: David J McCulley Erik A Jensen Jennifer M S Sucre Sarah McKenna Laura G Sherlock Evgenia Dobrinskikh Clyde J Wright Source Type: research

The impact of nebulized Epoprostenol and Iloprost on hemoglobin oxygen affinity - an ex-vivo experiment
Conclusion Prostacyclin-receptor stimulation and subsequent cAMP mediated ATP release from erythrocytes might explain the effect of epoprostenol on Hb-O2 affinity but the reason for the sex hormone modified iloprost effect remains unclear. This finding is of great interest, as contraception is recommended for women taking iloprost. Being aware of potentially differing effects on Hb-O2 affinity might help select the right prostacyclin (epoprostenol vs. iloprost) depending on the patient and the underlying disease (e.g., acute respiratory distress syndrome vs. peripheral arterial disease).PMID:35503651 | DOI:10.1152/ajplung....
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 3, 2022 Category: Cytology Authors: Simon Woyke Norbert Mair Thomas Haller Marco Ronzani David Plunser Herbert Oberacher Hannes Gatterer Christopher Rugg Mathias Str öhle Source Type: research