Diet-induced obesity in mice impairs host defense against Klebsiella pneumonia in vivo and glucose transport and bactericidal functions in neutrophils in vitro
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00008.2021. Online ahead of print.ABSTRACTObesity impairs host defense against Klebsiella pneumoniae but responsible mechanisms are incompletely understood. To determine the impact of diet-induced obesity on pulmonary host defense against K. pneumoniae, we fed 6-week-old male C57BL/6j mice a normal (ND) or high fat diet (HFD) (13% versus 60% fat, respectively) for 16 weeks. Mice were intratracheally infected with Klebsiella, assayed at 24 or 48 h for bacterial colony-forming units, lung cytokines, and leukocytes from alveolar spaces, lung parenchyma, and ...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Peter Mancuso Jeffrey L Curtis Anne Marie Weitzel Cameron A Griffin Benjamin Bouchard Christine M Freeman Dave Bridges Kanakadurga Singer Source Type: research

A Wrinkle in Time: Circadian biology in pulmonary vascular health and disease
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00037.2021. Online ahead of print.ABSTRACTAn often over looked element of pulmonary vascular disease is time. Cellular responses to time, which are regulated directly by the core circadian clock, have only recently been elucidated. Despite an extensive collection of data regarding the role of rhythmic contribution to disease pathogenesis (such as systemic hypertension, coronary artery and renal disease), the roles of key circadian transcription factors in pulmonary hypertension remain under-studied. This is despite a large degree of overlap in the pulmona...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Andrew J Bryant Elnaz Ebrahimi Amy Nguyen Christopher A Wolff Michelle L Gumz Andrew C Liu Karyn A Esser Source Type: research

Mouse Lung Organoid Responses to Reduced, Increased, and Cyclic Stretch
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00310.2020. Online ahead of print.ABSTRACTMost lung development occurs in the context of cyclic stretch. Alteration of the mechanical microenvironment is a common feature of many pulmonary diseases with congenital diaphragmatic hernia (CDH) and fetal tracheal occlusion (FETO, a therapy for CDH) being extreme examples with changes in lung structure, cell differentiation and function. To address limitations in cell culture and in vivo mechanotransductive models we developed two mouse lung organoid (mLO) mechanotransductive models using postnatal day 5 (PND5...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Rashika Joshi Matthew R Batie Qiang Fan Brian Michael Varisco Source Type: research

The highly packed and dehydrated structure of pre-formed unexposed human pulmonary surfactant isolated from amniotic fluid
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00230.2021. Online ahead of print.ABSTRACTBy coating the alveolar air-liquid interface, lung surfactant overwhelms surface tension forces that, otherwise, would hinder the lifetime effort of breathing. Years of research have provided a picture of how highly hydrophobic and specialized proteins in surfactant promote rapid and efficient formation of phospholipid-based complex three-dimensional films at the respiratory surface, highly stable under the demanding breathing mechanics. However, recent evidence suggest that the structure and performance of surfac...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Jos é Carlos Castillo-Sánchez Nuria Rold án Bego ña García-Álvarez Emma Batllori Alberto Galindo Antonio Cruz Jesus Perez-Gil Source Type: research

Short Palate, Lung, and Nasal Epithelial Clone1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00315.2021. Online ahead of print.ABSTRACTAsthma and its heterogeneity changes with age. Increased airspace neutrophil numbers contribute to severe steroid-resistant asthma exacerbation in the elderly, which correlates with the changes seen in adult asthmatics. However, whether that resembles the same disease mechanism and pathophysiology in aged and adults is poorly understood. Here, we sought to address the underlying molecular mechanism of steroid-resistant airway inflammation development and response to corticosteroid (Dex) therapy in aged mice. To st...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Anil Kumar Jaiswal Jyoti Yadav Sangeet Makhija Maninder Sandey Amol Suryawanshi Amit Kumar Mitra Amarjit Mishra Source Type: research

BMP4 and WNT signaling interact to promote mouse tracheal mesenchyme morphogenesis
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00255.2021. Online ahead of print.ABSTRACTTracheobronchomalacia and Complete Tracheal Rings are congenital malformations of the trachea associated with morbidity and mortality for which the etiology remains poorly understood. Epithelial expression of Wls (a cargo receptor mediating Wnt ligand secretion) by tracheal cells is essential for patterning the embryonic mouse trachea's cartilage and muscle. RNA sequencing indicated that Wls differentially modulated the expression of BMP signaling molecules. We tested whether BMP signaling, induced by epithelial W...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Natalia Bottasso Arias Lauren Leesman Kaulini Burra John Snowball Ronak M Shah Megha Mohanakrishnan Yan Xu Debora Sinner Source Type: research

Diet-induced obesity in mice impairs host defense against Klebsiella pneumonia in vivo and glucose transport and bactericidal functions in neutrophils in vitro
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00008.2021. Online ahead of print.ABSTRACTObesity impairs host defense against Klebsiella pneumoniae but responsible mechanisms are incompletely understood. To determine the impact of diet-induced obesity on pulmonary host defense against K. pneumoniae, we fed 6-week-old male C57BL/6j mice a normal (ND) or high fat diet (HFD) (13% versus 60% fat, respectively) for 16 weeks. Mice were intratracheally infected with Klebsiella, assayed at 24 or 48 h for bacterial colony-forming units, lung cytokines, and leukocytes from alveolar spaces, lung parenchyma, and ...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Peter Mancuso Jeffrey L Curtis Anne Marie Weitzel Cameron A Griffin Benjamin Bouchard Christine M Freeman Dave Bridges Kanakadurga Singer Source Type: research

A Wrinkle in Time: Circadian biology in pulmonary vascular health and disease
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00037.2021. Online ahead of print.ABSTRACTAn often over looked element of pulmonary vascular disease is time. Cellular responses to time, which are regulated directly by the core circadian clock, have only recently been elucidated. Despite an extensive collection of data regarding the role of rhythmic contribution to disease pathogenesis (such as systemic hypertension, coronary artery and renal disease), the roles of key circadian transcription factors in pulmonary hypertension remain under-studied. This is despite a large degree of overlap in the pulmona...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Andrew J Bryant Elnaz Ebrahimi Amy Nguyen Christopher A Wolff Michelle L Gumz Andrew C Liu Karyn A Esser Source Type: research

Mouse Lung Organoid Responses to Reduced, Increased, and Cyclic Stretch
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00310.2020. Online ahead of print.ABSTRACTMost lung development occurs in the context of cyclic stretch. Alteration of the mechanical microenvironment is a common feature of many pulmonary diseases with congenital diaphragmatic hernia (CDH) and fetal tracheal occlusion (FETO, a therapy for CDH) being extreme examples with changes in lung structure, cell differentiation and function. To address limitations in cell culture and in vivo mechanotransductive models we developed two mouse lung organoid (mLO) mechanotransductive models using postnatal day 5 (PND5...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Rashika Joshi Matthew R Batie Qiang Fan Brian Michael Varisco Source Type: research

The highly packed and dehydrated structure of pre-formed unexposed human pulmonary surfactant isolated from amniotic fluid
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00230.2021. Online ahead of print.ABSTRACTBy coating the alveolar air-liquid interface, lung surfactant overwhelms surface tension forces that, otherwise, would hinder the lifetime effort of breathing. Years of research have provided a picture of how highly hydrophobic and specialized proteins in surfactant promote rapid and efficient formation of phospholipid-based complex three-dimensional films at the respiratory surface, highly stable under the demanding breathing mechanics. However, recent evidence suggest that the structure and performance of surfac...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Jos é Carlos Castillo-Sánchez Nuria Rold án Bego ña García-Álvarez Emma Batllori Alberto Galindo Antonio Cruz Jesus Perez-Gil Source Type: research

Short Palate, Lung, and Nasal Epithelial Clone1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00315.2021. Online ahead of print.ABSTRACTAsthma and its heterogeneity changes with age. Increased airspace neutrophil numbers contribute to severe steroid-resistant asthma exacerbation in the elderly, which correlates with the changes seen in adult asthmatics. However, whether that resembles the same disease mechanism and pathophysiology in aged and adults is poorly understood. Here, we sought to address the underlying molecular mechanism of steroid-resistant airway inflammation development and response to corticosteroid (Dex) therapy in aged mice. To st...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Anil Kumar Jaiswal Jyoti Yadav Sangeet Makhija Maninder Sandey Amol Suryawanshi Amit Kumar Mitra Amarjit Mishra Source Type: research

BMP4 and WNT signaling interact to promote mouse tracheal mesenchyme morphogenesis
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00255.2021. Online ahead of print.ABSTRACTTracheobronchomalacia and Complete Tracheal Rings are congenital malformations of the trachea associated with morbidity and mortality for which the etiology remains poorly understood. Epithelial expression of Wls (a cargo receptor mediating Wnt ligand secretion) by tracheal cells is essential for patterning the embryonic mouse trachea's cartilage and muscle. RNA sequencing indicated that Wls differentially modulated the expression of BMP signaling molecules. We tested whether BMP signaling, induced by epithelial W...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Natalia Bottasso Arias Lauren Leesman Kaulini Burra John Snowball Ronak M Shah Megha Mohanakrishnan Yan Xu Debora Sinner Source Type: research

Diet-induced obesity in mice impairs host defense against Klebsiella pneumonia in vivo and glucose transport and bactericidal functions in neutrophils in vitro
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00008.2021. Online ahead of print.ABSTRACTObesity impairs host defense against Klebsiella pneumoniae but responsible mechanisms are incompletely understood. To determine the impact of diet-induced obesity on pulmonary host defense against K. pneumoniae, we fed 6-week-old male C57BL/6j mice a normal (ND) or high fat diet (HFD) (13% versus 60% fat, respectively) for 16 weeks. Mice were intratracheally infected with Klebsiella, assayed at 24 or 48 h for bacterial colony-forming units, lung cytokines, and leukocytes from alveolar spaces, lung parenchyma, and ...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Peter Mancuso Jeffrey L Curtis Anne Marie Weitzel Cameron A Griffin Benjamin Bouchard Christine M Freeman Dave Bridges Kanakadurga Singer Source Type: research

A Wrinkle in Time: Circadian biology in pulmonary vascular health and disease
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00037.2021. Online ahead of print.ABSTRACTAn often over looked element of pulmonary vascular disease is time. Cellular responses to time, which are regulated directly by the core circadian clock, have only recently been elucidated. Despite an extensive collection of data regarding the role of rhythmic contribution to disease pathogenesis (such as systemic hypertension, coronary artery and renal disease), the roles of key circadian transcription factors in pulmonary hypertension remain under-studied. This is despite a large degree of overlap in the pulmona...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Andrew J Bryant Elnaz Ebrahimi Amy Nguyen Christopher A Wolff Michelle L Gumz Andrew C Liu Karyn A Esser Source Type: research

Mouse Lung Organoid Responses to Reduced, Increased, and Cyclic Stretch
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00310.2020. Online ahead of print.ABSTRACTMost lung development occurs in the context of cyclic stretch. Alteration of the mechanical microenvironment is a common feature of many pulmonary diseases with congenital diaphragmatic hernia (CDH) and fetal tracheal occlusion (FETO, a therapy for CDH) being extreme examples with changes in lung structure, cell differentiation and function. To address limitations in cell culture and in vivo mechanotransductive models we developed two mouse lung organoid (mLO) mechanotransductive models using postnatal day 5 (PND5...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Rashika Joshi Matthew R Batie Qiang Fan Brian Michael Varisco Source Type: research

The highly packed and dehydrated structure of pre-formed unexposed human pulmonary surfactant isolated from amniotic fluid
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00230.2021. Online ahead of print.ABSTRACTBy coating the alveolar air-liquid interface, lung surfactant overwhelms surface tension forces that, otherwise, would hinder the lifetime effort of breathing. Years of research have provided a picture of how highly hydrophobic and specialized proteins in surfactant promote rapid and efficient formation of phospholipid-based complex three-dimensional films at the respiratory surface, highly stable under the demanding breathing mechanics. However, recent evidence suggest that the structure and performance of surfac...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Jos é Carlos Castillo-Sánchez Nuria Rold án Bego ña García-Álvarez Emma Batllori Alberto Galindo Antonio Cruz Jesus Perez-Gil Source Type: research

Short Palate, Lung, and Nasal Epithelial Clone1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00315.2021. Online ahead of print.ABSTRACTAsthma and its heterogeneity changes with age. Increased airspace neutrophil numbers contribute to severe steroid-resistant asthma exacerbation in the elderly, which correlates with the changes seen in adult asthmatics. However, whether that resembles the same disease mechanism and pathophysiology in aged and adults is poorly understood. Here, we sought to address the underlying molecular mechanism of steroid-resistant airway inflammation development and response to corticosteroid (Dex) therapy in aged mice. To st...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Anil Kumar Jaiswal Jyoti Yadav Sangeet Makhija Maninder Sandey Amol Suryawanshi Amit Kumar Mitra Amarjit Mishra Source Type: research

BMP4 and WNT signaling interact to promote mouse tracheal mesenchyme morphogenesis
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1. doi: 10.1152/ajplung.00255.2021. Online ahead of print.ABSTRACTTracheobronchomalacia and Complete Tracheal Rings are congenital malformations of the trachea associated with morbidity and mortality for which the etiology remains poorly understood. Epithelial expression of Wls (a cargo receptor mediating Wnt ligand secretion) by tracheal cells is essential for patterning the embryonic mouse trachea's cartilage and muscle. RNA sequencing indicated that Wls differentially modulated the expression of BMP signaling molecules. We tested whether BMP signaling, induced by epithelial W...
Source: Am J Physiol Lung Ce... - December 1, 2021 Category: Respiratory Medicine Authors: Natalia Bottasso Arias Lauren Leesman Kaulini Burra John Snowball Ronak M Shah Megha Mohanakrishnan Yan Xu Debora Sinner Source Type: research

World AIDS Day 2021: highlighting the pulmonary complications of HIV/AIDS
Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1;321(6):L1069-L1071. doi: 10.1152/ajplung.00471.2021. Epub 2021 Nov 24.NO ABSTRACTPMID:34816744 | DOI:10.1152/ajplung.00471.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 24, 2021 Category: Respiratory Medicine Authors: Rory E Morty Alison Morris Source Type: research

World AIDS Day 2021: highlighting the pulmonary complications of HIV/AIDS
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 24. doi: 10.1152/ajplung.00471.2021. Online ahead of print.NO ABSTRACTPMID:34816744 | DOI:10.1152/ajplung.00471.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 24, 2021 Category: Respiratory Medicine Authors: Rory E Morty Alison Morris Source Type: research

World AIDS Day 2021: highlighting the pulmonary complications of HIV/AIDS
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 24. doi: 10.1152/ajplung.00471.2021. Online ahead of print.NO ABSTRACTPMID:34816744 | DOI:10.1152/ajplung.00471.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 24, 2021 Category: Respiratory Medicine Authors: Rory E Morty Alison Morris Source Type: research

World AIDS Day 2021: highlighting the pulmonary complications of HIV/AIDS
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 24. doi: 10.1152/ajplung.00471.2021. Online ahead of print.NO ABSTRACTPMID:34816744 | DOI:10.1152/ajplung.00471.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 24, 2021 Category: Respiratory Medicine Authors: Rory E Morty Alison Morris Source Type: research

World AIDS Day 2021: highlighting the pulmonary complications of HIV/AIDS
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 24. doi: 10.1152/ajplung.00471.2021. Online ahead of print.NO ABSTRACTPMID:34816744 | DOI:10.1152/ajplung.00471.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 24, 2021 Category: Respiratory Medicine Authors: Rory E Morty Alison Morris Source Type: research

World AIDS Day 2021: highlighting the pulmonary complications of HIV/AIDS
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 24. doi: 10.1152/ajplung.00471.2021. Online ahead of print.NO ABSTRACTPMID:34816744 | DOI:10.1152/ajplung.00471.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 24, 2021 Category: Respiratory Medicine Authors: Rory E Morty Alison Morris Source Type: research

A new anesthesia protocol enabling longitudinal lung function measurements in neonatal rabbits by micro-CT
In conclusion, we have identified a safe and suitable anesthesia protocol for micro-CT imaging in neonatal rabbits. Moreover, the possibility to longitudinally measure lung function in the same subject dramatically reduced the intra-experimental variability.PMID:34786984 | DOI:10.1152/ajplung.00328.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Erica Ferrini Ludovica Leo Luisa Corsi Chiara Catozzi Fabrizio Salomone Luisa Ragionieri Francesca Pennati Franco Fabio Stellari Source Type: research

Inspired oxygen: present, past, and future
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00297.2021. Online ahead of print.ABSTRACTAs earthlings, we take the oxygen in the air that we breathe for granted. Few people realize that this easy access to oxygen makes us unique in the whole universe. Nowhere else in our planetary system or in distant stars has stable oxygen ever been detected. However, the present plentiful supply of oxygen in our atmosphere was not always there. Long after the earth was formed some 4.5 billion years ago, the PO2 in the atmosphere was near zero, and it remained so for millions of years. But about 2 billion years ag...
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: John B West Source Type: research

Ending AIDS and Stopping Pandemics Through Closing Inequalities
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00463.2021. Online ahead of print.NO ABSTRACTPMID:34786993 | DOI:10.1152/ajplung.00463.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Matthew M Kavanagh Helena Nygren Krug Source Type: research

A new anesthesia protocol enabling longitudinal lung function measurements in neonatal rabbits by micro-CT
In conclusion, we have identified a safe and suitable anesthesia protocol for micro-CT imaging in neonatal rabbits. Moreover, the possibility to longitudinally measure lung function in the same subject dramatically reduced the intra-experimental variability.PMID:34786984 | DOI:10.1152/ajplung.00328.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Erica Ferrini Ludovica Leo Luisa Corsi Chiara Catozzi Fabrizio Salomone Luisa Ragionieri Francesca Pennati Franco Fabio Stellari Source Type: research

Inspired oxygen: present, past, and future
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00297.2021. Online ahead of print.ABSTRACTAs earthlings, we take the oxygen in the air that we breathe for granted. Few people realize that this easy access to oxygen makes us unique in the whole universe. Nowhere else in our planetary system or in distant stars has stable oxygen ever been detected. However, the present plentiful supply of oxygen in our atmosphere was not always there. Long after the earth was formed some 4.5 billion years ago, the PO2 in the atmosphere was near zero, and it remained so for millions of years. But about 2 billion years ag...
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: John B West Source Type: research

Ending AIDS and Stopping Pandemics Through Closing Inequalities
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00463.2021. Online ahead of print.NO ABSTRACTPMID:34786993 | DOI:10.1152/ajplung.00463.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Matthew M Kavanagh Helena Nygren Krug Source Type: research

A new anesthesia protocol enabling longitudinal lung function measurements in neonatal rabbits by micro-CT
In conclusion, we have identified a safe and suitable anesthesia protocol for micro-CT imaging in neonatal rabbits. Moreover, the possibility to longitudinally measure lung function in the same subject dramatically reduced the intra-experimental variability.PMID:34786984 | DOI:10.1152/ajplung.00328.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Erica Ferrini Ludovica Leo Luisa Corsi Chiara Catozzi Fabrizio Salomone Luisa Ragionieri Francesca Pennati Franco Fabio Stellari Source Type: research

Inspired oxygen: present, past, and future
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00297.2021. Online ahead of print.ABSTRACTAs earthlings, we take the oxygen in the air that we breathe for granted. Few people realize that this easy access to oxygen makes us unique in the whole universe. Nowhere else in our planetary system or in distant stars has stable oxygen ever been detected. However, the present plentiful supply of oxygen in our atmosphere was not always there. Long after the earth was formed some 4.5 billion years ago, the PO2 in the atmosphere was near zero, and it remained so for millions of years. But about 2 billion years ag...
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: John B West Source Type: research

Ending AIDS and Stopping Pandemics Through Closing Inequalities
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00463.2021. Online ahead of print.NO ABSTRACTPMID:34786993 | DOI:10.1152/ajplung.00463.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Matthew M Kavanagh Helena Nygren Krug Source Type: research

A new anesthesia protocol enabling longitudinal lung function measurements in neonatal rabbits by micro-CT
In conclusion, we have identified a safe and suitable anesthesia protocol for micro-CT imaging in neonatal rabbits. Moreover, the possibility to longitudinally measure lung function in the same subject dramatically reduced the intra-experimental variability.PMID:34786984 | DOI:10.1152/ajplung.00328.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Erica Ferrini Ludovica Leo Luisa Corsi Chiara Catozzi Fabrizio Salomone Luisa Ragionieri Francesca Pennati Franco Fabio Stellari Source Type: research

Inspired oxygen: present, past, and future
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00297.2021. Online ahead of print.ABSTRACTAs earthlings, we take the oxygen in the air that we breathe for granted. Few people realize that this easy access to oxygen makes us unique in the whole universe. Nowhere else in our planetary system or in distant stars has stable oxygen ever been detected. However, the present plentiful supply of oxygen in our atmosphere was not always there. Long after the earth was formed some 4.5 billion years ago, the PO2 in the atmosphere was near zero, and it remained so for millions of years. But about 2 billion years ag...
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: John B West Source Type: research

Ending AIDS and Stopping Pandemics Through Closing Inequalities
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00463.2021. Online ahead of print.NO ABSTRACTPMID:34786993 | DOI:10.1152/ajplung.00463.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Matthew M Kavanagh Helena Nygren Krug Source Type: research

A new anesthesia protocol enabling longitudinal lung function measurements in neonatal rabbits by micro-CT
In conclusion, we have identified a safe and suitable anesthesia protocol for micro-CT imaging in neonatal rabbits. Moreover, the possibility to longitudinally measure lung function in the same subject dramatically reduced the intra-experimental variability.PMID:34786984 | DOI:10.1152/ajplung.00328.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Erica Ferrini Ludovica Leo Luisa Corsi Chiara Catozzi Fabrizio Salomone Luisa Ragionieri Francesca Pennati Franco Fabio Stellari Source Type: research

Inspired oxygen: present, past, and future
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00297.2021. Online ahead of print.ABSTRACTAs earthlings, we take the oxygen in the air that we breathe for granted. Few people realize that this easy access to oxygen makes us unique in the whole universe. Nowhere else in our planetary system or in distant stars has stable oxygen ever been detected. However, the present plentiful supply of oxygen in our atmosphere was not always there. Long after the earth was formed some 4.5 billion years ago, the PO2 in the atmosphere was near zero, and it remained so for millions of years. But about 2 billion years ag...
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: John B West Source Type: research

Ending AIDS and Stopping Pandemics Through Closing Inequalities
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00463.2021. Online ahead of print.NO ABSTRACTPMID:34786993 | DOI:10.1152/ajplung.00463.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Matthew M Kavanagh Helena Nygren Krug Source Type: research

A new anesthesia protocol enabling longitudinal lung function measurements in neonatal rabbits by micro-CT
In conclusion, we have identified a safe and suitable anesthesia protocol for micro-CT imaging in neonatal rabbits. Moreover, the possibility to longitudinally measure lung function in the same subject dramatically reduced the intra-experimental variability.PMID:34786984 | DOI:10.1152/ajplung.00328.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Erica Ferrini Ludovica Leo Luisa Corsi Chiara Catozzi Fabrizio Salomone Luisa Ragionieri Francesca Pennati Franco Fabio Stellari Source Type: research

Inspired oxygen: present, past, and future
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00297.2021. Online ahead of print.ABSTRACTAs earthlings, we take the oxygen in the air that we breathe for granted. Few people realize that this easy access to oxygen makes us unique in the whole universe. Nowhere else in our planetary system or in distant stars has stable oxygen ever been detected. However, the present plentiful supply of oxygen in our atmosphere was not always there. Long after the earth was formed some 4.5 billion years ago, the PO2 in the atmosphere was near zero, and it remained so for millions of years. But about 2 billion years ag...
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: John B West Source Type: research

Ending AIDS and Stopping Pandemics Through Closing Inequalities
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 17. doi: 10.1152/ajplung.00463.2021. Online ahead of print.NO ABSTRACTPMID:34786993 | DOI:10.1152/ajplung.00463.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 17, 2021 Category: Respiratory Medicine Authors: Matthew M Kavanagh Helena Nygren Krug Source Type: research

Combined control of the fibroblast contractile program by YAP and TAZ
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00210.2021. Online ahead of print.ABSTRACTYes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) are transcription cofactors implicated in the contractile and pro-fibrotic activation of fibroblasts. Fibroblast contractile function is important in alveologenesis, and in lung wound healing and fibrosis. As paralogs, YAP and TAZ may have independent or redundant roles in regulating transcriptional programs and contractile function. Using IMR-90 lung fibroblasts, microarray analysis, and traction microscopy, we tested whet...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Patrick Adam Link Kyoung Moo Choi Ana M Diaz Espinosa Dakota L Jones Ashley Y Gao Andrew J Haak Daniel J Tschumperlin Source Type: research

Human Immunodeficiency Viruses and its effect on the Pulmonary Vascular bed
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00451.2021. Online ahead of print.ABSTRACTDecember. 1st 2021 is "World AIDS Day" reminding us that HIV infection is still widespread and that many of its long-term effects can be deadly. One of these complications is its effect on the pulmonary vascular beds, leading to an increase in the pulmonary pressure, causing the clinical manifestation of "pulmonary hypertension". Unfortunately, we are still far from fully understanding the prevalence, mechanics, and pathobiology of "HIV pulmonary hypertension", especially in Africa a...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Ghazwan Butrous Source Type: research

Identification of Lung Innervating Sensory Neurons and Their Target Specificity
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00376.2021. Online ahead of print.ABSTRACTKnown as the gas exchange organ, the lung is also critical for responding to the aerosol environment in part through interaction with the nervous system. The diversity and specificity of lung innervating neurons remains poorly understood. Here, we interrogated the cell body location, molecular signature and projection pattern of lung innervating sensory neurons. Retrograde tracing from the lung coupled with whole tissue clearing highlighted neurons primarily in the vagal ganglia. Centrally, they project specifica...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Yujuan Su Justinn Barr Abigail Jaquish Jinhao Xu Jamie M Verheyden Xin Sun Source Type: research

Integrative analysis reveals mouse strain-dependent responses to acute ozone exposure associated with airway macrophage transcriptional activity
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00237.2021. Online ahead of print.ABSTRACTAcute ozone (O3) exposure is associated with multiple adverse cardiorespiratory outcomes, the severity of which varies across individuals in human populations and inbred mouse strains. However, molecular determinants of response, including susceptibility biomarkers that distinguish who will develop severe injury and inflammation, are not well characterized. We and others have demonstrated that airway macrophages (AMs) are an important resident immune cell type that are functionally and transcriptionally responsiv...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Adelaide Tovar Wesley L Crouse Gregory J Smith Joseph M Thomas Benjamin P Keith Kathryn M McFadden Timothy P Moran Terrence S Furey Samir N P Kelada Source Type: research

Corticosteroid Insensitivity Persists in the Absence of STAT1 Signaling in Severe Allergic Airway Inflammation
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00244.2021. Online ahead of print.ABSTRACTCorticosteroid insensitivity in asthma limits the ability to effectively manage severe asthma, which is characterized by persistent airway inflammation, airway hyperresponsiveness (AHR), and airflow obstruction despite corticosteroid treatment. Recent reports indicate that corticosteroid insensitivity is associated with increased interferon-gamma (IFN-g) levels and T-helper (Th) 1 lymphocyte infiltration in severe asthma. Signal Transducer and Activator of Transcription 1 (STAT1) activation by IFN-g is a key sign...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Brandon W Lewis Devine Jackson Stephanie A Amici Joshua Walum Manel Guessas Sonia Guessas Elise Coneglio Akhila V Boda Mieria Guerau-de-Arellano Mitchell H Grayson Rodney D Britt Source Type: research

World AIDS Day: 40 Years of an Evolving Pulmonary Landscape
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00457.2021. Online ahead of print.NO ABSTRACTPMID:34755570 | DOI:10.1152/ajplung.00457.2021 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Kristina Crothers Lynn Schnapp Source Type: research

Combined control of the fibroblast contractile program by YAP and TAZ
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00210.2021. Online ahead of print.ABSTRACTYes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) are transcription cofactors implicated in the contractile and pro-fibrotic activation of fibroblasts. Fibroblast contractile function is important in alveologenesis, and in lung wound healing and fibrosis. As paralogs, YAP and TAZ may have independent or redundant roles in regulating transcriptional programs and contractile function. Using IMR-90 lung fibroblasts, microarray analysis, and traction microscopy, we tested whet...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Patrick Adam Link Kyoung Moo Choi Ana M Diaz Espinosa Dakota L Jones Ashley Y Gao Andrew J Haak Daniel J Tschumperlin Source Type: research

Human Immunodeficiency Viruses and its effect on the Pulmonary Vascular bed
Am J Physiol Lung Cell Mol Physiol. 2021 Nov 10. doi: 10.1152/ajplung.00451.2021. Online ahead of print.ABSTRACTDecember. 1st 2021 is "World AIDS Day" reminding us that HIV infection is still widespread and that many of its long-term effects can be deadly. One of these complications is its effect on the pulmonary vascular beds, leading to an increase in the pulmonary pressure, causing the clinical manifestation of "pulmonary hypertension". Unfortunately, we are still far from fully understanding the prevalence, mechanics, and pathobiology of "HIV pulmonary hypertension", especially in Africa a...
Source: Am J Physiol Lung Ce... - November 10, 2021 Category: Respiratory Medicine Authors: Ghazwan Butrous Source Type: research