Selection of bifunctional RNAs with specificity for arginine and lipid membranes
Here, we constructed a bifunctional RNA 10Arg aptamer that is specific for arginine and lipids. The selection-amplification method yielded several RNA 10Arg(D) sequences with improved affinity for arginine and liposomes. Generation of these bispecific RNAs supports the hypothesis that an RNA molecule can bind both to RNA-binding proteins through arginine and to membrane lipid rafts, thus facilitating RNA loading into extracellular vesicles. The molecular mechanisms of selective RNA loading into exosomes and other extracellular vesicles are not yet completely understood. In order to show that a pool of RNA sequences binds b...
Source: FEBS Letters - April 23, 2024 Category: Biochemistry Authors: Teresa Janas,
Karolina Sapo ń,
Tadeusz Janas Tags: Research Article Source Type: research
METTL14 promotes neuroblastoma formation by inhibiting YWHAH via an m6A-YTHDF1-dependent mechanism
Cell Death Discov. 2024 Apr 22;10(1):186. doi: 10.1038/s41420-024-01959-8.ABSTRACTNeuroblastoma (NB) is a common childhood tumor with a high incidence worldwide. The regulatory role of RNA N6-methyladenosine (m6A) in gene expression has attracted significant attention, and the impact of methyltransferase-like 14 (METTL14) on tumor progression has been extensively studied in various types of cancer. However, the specific influence of METTL14 on NB remains unexplored. Using data from the Target database, our study revealed significant upregulation of METTL14 expression in high-risk NB patients, with strong correlation with p...
Source: Cancer Control - April 22, 2024 Category: Cancer & Oncology Authors: Jianwei Wang Hongli Yin Gen Li Di Wu Yunyun Xu Yanling Chen Xiaodong Wang Yujiao Xing Ting Zhang Danhong Fei Pengcheng Yang Fang Fang Yanfang Tao Xiaolu Li Juanjuan Yu Yang Yang Zhiheng Li Lei Shi Zimu Zhang Jian Pan Source Type: research
Dopamine ‑iron homeostasis interaction rescues mitochondrial fitness in Parkinson's disease
In this study, we investigated the interplay between iron, dopamine, and mitochondrial activity in neuroblastoma SH-SY5Y cells and human induced pluripotent stem cell (hiPSC)-derived dopaminergic neurons differentiated from a healthy control and a PD patient with a mutation in the α-synuclein (SNCA) gene. In SH-SY5Y cells, dopamine treatment resulted in increased expression of the transmembrane iron transporters transferrin receptor 1 (TFR1), ferroportin (FPN), and mitoferrin2 (MFRN2) and intracellular iron accumulation, suggesting that dopamine may promote iron uptake. Furthermore, dopamine supplementation led to reduced...
Source: Neurobiology of Disease - April 22, 2024 Category: Neurology Authors: Chiara Buoso Markus Seifert Martin Lang Corey M Griffith Bego ña Talavera Andújar Maria Paulina Castelo Rueda Christine Fischer Carolina Doerrier Heribert Talasz Alessandra Zanon Peter P Pramstaller Emma L Schymanski Irene Pichler Guenter Weiss Source Type: research
Dopamine ‑iron homeostasis interaction rescues mitochondrial fitness in Parkinson's disease
In this study, we investigated the interplay between iron, dopamine, and mitochondrial activity in neuroblastoma SH-SY5Y cells and human induced pluripotent stem cell (hiPSC)-derived dopaminergic neurons differentiated from a healthy control and a PD patient with a mutation in the α-synuclein (SNCA) gene. In SH-SY5Y cells, dopamine treatment resulted in increased expression of the transmembrane iron transporters transferrin receptor 1 (TFR1), ferroportin (FPN), and mitoferrin2 (MFRN2) and intracellular iron accumulation, suggesting that dopamine may promote iron uptake. Furthermore, dopamine supplementation led to reduced...
Source: Neurobiology of Disease - April 22, 2024 Category: Neurology Authors: Chiara Buoso Markus Seifert Martin Lang Corey M Griffith Bego ña Talavera Andújar Maria Paulina Castelo Rueda Christine Fischer Carolina Doerrier Heribert Talasz Alessandra Zanon Peter P Pramstaller Emma L Schymanski Irene Pichler Guenter Weiss Source Type: research
Dopamine ‑iron homeostasis interaction rescues mitochondrial fitness in Parkinson's disease
In this study, we investigated the interplay between iron, dopamine, and mitochondrial activity in neuroblastoma SH-SY5Y cells and human induced pluripotent stem cell (hiPSC)-derived dopaminergic neurons differentiated from a healthy control and a PD patient with a mutation in the α-synuclein (SNCA) gene. In SH-SY5Y cells, dopamine treatment resulted in increased expression of the transmembrane iron transporters transferrin receptor 1 (TFR1), ferroportin (FPN), and mitoferrin2 (MFRN2) and intracellular iron accumulation, suggesting that dopamine may promote iron uptake. Furthermore, dopamine supplementation led to reduced...
Source: Neurobiology of Disease - April 22, 2024 Category: Neurology Authors: Chiara Buoso Markus Seifert Martin Lang Corey M Griffith Bego ña Talavera Andújar Maria Paulina Castelo Rueda Christine Fischer Carolina Doerrier Heribert Talasz Alessandra Zanon Peter P Pramstaller Emma L Schymanski Irene Pichler Guenter Weiss Source Type: research
Cancers, Vol. 16, Pages 1590: Unsaturated Fatty Acid Synthesis Is Associated with Worse Survival and Is Differentially Regulated by MYCN and Tumor Suppressor microRNAs in Neuroblastoma
John T. Powers
MYCN amplification (MNA) and disruption of tumor suppressor microRNA (TSmiR) function are key drivers of poor outcomes in neuroblastoma (NB). While MYCN and TSmiRs regulate glucose metabolism, their role in de novo fatty acid synthesis (FAS) and unsaturated FAS (UFAS) remains poorly understood. Here, we show that FAS and UFAS (U/FAS) genes FASN, ELOVL6, SCD, FADS2, and FADS1 are upregulated in high-risk (HR) NB and that their expression is associated with lower overall survival. RNA-Seq analysis of human NB cell lines revealed parallel U/FAS gene expression patterns. Consistent with this, we found tha...
Source: Cancers - April 21, 2024 Category: Cancer & Oncology Authors: Dennis A. Sheeter Secilia Garza Hui Gyu Park Lorraine-Rana E. Benhamou Niharika R. Badi Erika C. Espinosa Kumar S. D. Kothapalli J. Thomas Brenna John T. Powers Tags: Article Source Type: research
Effects of Petasitin as Natural Extract on Proliferation and Pathological Changes of Pediatric Neuroblastoma SK-N-SH Cells
(Source: Journal of Biomedical Nanotechnology)
Source: Journal of Biomedical Nanotechnology - April 20, 2024 Category: Nanotechnology Authors: Li, Ling Zheng, Mingjun Meng, Weixia Tags: research-article Source Type: research
Cancers, Vol. 16, Pages 1520: Identification and In Vivo Validation of Unique Anti-Oncogenic Mechanisms Involving Protein Kinase Signaling and Autophagy Mediated by the Investigational Agent PV-10
Narendran
PV-10 is a 10% formulation of rose bengal sodium that has potent immunotherapeutic and anti-cancer activity against various tumors, including metastatic melanoma and refractory neuroblastoma. Currently, PV-10 is undergoing clinical testing for refractory metastatic neuroendocrine cancer and melanomas. However, preclinical investigation of PV-10 activity and its mechanisms against phenotypically and molecularly diverse adult solid tumors had not been conducted. In a panel of human cell lines derived from breast, colorectal, head and neck, and testicular cancers, we demonstrated that PV-10 induces cytotoxicity ...
Source: Cancers - April 16, 2024 Category: Cancer & Oncology Authors: Son Tran Patrick Sipila Satbir Thakur Chunfen Zhang Aru Narendran Tags: Article Source Type: research
Correlation of BARD1 gene polymorphisms with risk of neuroblastoma: a meta-analysis
Nucleosides Nucleotides Nucleic Acids. 2024 Apr 15:1-19. doi: 10.1080/15257770.2024.2336215. Online ahead of print.ABSTRACTBRCA1-associated RING domain protein 1 (BARD1) gene polymorphisms may be associated with neuroblastoma (NB) susceptibility. However, the results remain controversial. Relevant studies were identified by searching PubMed, Web of Science, Embase, China National Knowledge Infrastructure databases up to March 5, 2023. The strength of the association between BARD1 polymorphisms and susceptibility of NB was assessed by calculating odds ratios (ORs) and 95% confidence intervals (95% CIs) through the fixed- or...
Source: Nucleosides, Nucleotides and Nucleic Acids - April 15, 2024 Category: Biochemistry Authors: Shan Chen Di Xu Rongdong Huang Yang Lin Lizhi Li Source Type: research
Molecules, Vol. 29, Pages 1782: Design, Synthesis, and Biological Evaluation of Novel Tetrahydroacridin Hybrids with Sulfur-Inserted Linkers as Potential Multitarget Agents for Alzheimer & rsquo;s Disease
Molecules, Vol. 29, Pages 1782: Design, Synthesis, and Biological Evaluation of Novel Tetrahydroacridin Hybrids with Sulfur-Inserted Linkers as Potential Multitarget Agents for Alzheimer’s Disease
Molecules doi: 10.3390/molecules29081782
Authors:
Xiuyuan Wu
Xiaotong Ze
Shuai Qin
Beiyu Zhang
Xinnan Li
Qi Gong
Haiyan Zhang
Zheying Zhu
Jinyi Xu
Alzheimer’s disease (AD) is a complex neurodegenerative disease that can lead to the loss of cognitive function. The progression of AD is regulated by multiple signaling pathways and their associated targets. Therefore, multitarget strate...
Source: Molecules - April 14, 2024 Category: Chemistry Authors: Xiuyuan Wu Xiaotong Ze Shuai Qin Beiyu Zhang Xinnan Li Qi Gong Haiyan Zhang Zheying Zhu Jinyi Xu Tags: Article Source Type: research
Diagnostic value of 18F-fluorodeoxyglucose positron emission tomography/computed tomography imaging in pediatric opsoclonus myoclonus ataxia syndrome presenting with neuroblastoma
ConclusionIn patients with OMAS,18F-FDG PET/CT has a high diagnostic accuracy in detecting tumors of the neuroblastoma, especially when combined with the independent risk factor serum lactate dehydrogenase.Graphical abstract (Source: Pediatric Radiology)
Source: Pediatric Radiology - April 13, 2024 Category: Radiology Source Type: research
Predictive value of 18 F-FDG PET/CT versus bone marrow biopsy and aspiration in pediatric neuroblastoma
Conclusion18 F-FDG-PET/CT is a valuable method for evaluating BMM in NB. The routine practice of performing a BMBA without discrimination may need to be reassessed. Negative result from 18 F-FDG-PET/CT could potentially spare children with invasive bone marrow biopsies. (Source: Clinical and Experimental Metastasis)
Source: Clinical and Experimental Metastasis - April 13, 2024 Category: Cancer & Oncology Source Type: research
