Transcriptional shifts in delignification ‐defective mutants of the white‐rot fungus Pleurotus ostreatus
In this study, we conducted a comparative transcriptome analysis to identify genes that are differentially expressed in ligninolysis ‐deficient mutant strains. Putative ligninolytic genes that are highly expressed in parental strains are significantly downregulated in the mutant strains. On the contrary, many putative cellulolytic and xylanolytic genes are upregulated in thechd1 ‐1, Δpex1, and Δgat1 strains. Identifying transcriptional alterations in mutant strains could provide new insights into the regulatory mechanisms of lignocellulolytic genes inP.  ostreatus. (Source: FEBS Letters)
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: Hongli Wu, Takehito Nakazawa, Atsuki Takenaka, Rina Kodera, Ryota Morimoto, Masahiro Sakamoto, Yoichi Honda Tags: Research Article Source Type: research

Complement evasion strategies of Borrelia burgdorferi sensu lato
AbstractBorreliosis (Lyme disease) is a spirochetal disease caused by the species complex ofBorrelia burgdorferi transmitted byIxodes spp. ticks. Recorded to be the most common tick ‐borne disease in the world, the last two decades have seen an increase in disease incidence and distribution, exceeding 360 000 cases in Europe alone. If untreated, infection may cause skin symptoms, arthritis, neurological or cardiac complications. Borrelia spirochetes have developed strategies to evade the mammalian host immune system. These include the complement system, which is an important first‐line defence mechanism against invadin...
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: V Dulipati, S Meri, J Panelius Tags: REVIEW Source Type: research

CTRP12 inhibits triglyceride synthesis and export in hepatocytes by suppressing HNF ‐4α and DGAT2 expression
AbstractCTRP12 is an anti ‐diabetic adipokine whose circulating levels are reduced in obesity and diabetes. Although partial and complete loss‐of‐function mouse models suggest a role for CTRP12 in modulating lipid metabolism and adiposity, its effect on cellular lipid metabolism remains poorly defined. Here, we demonst rate a direct action of CTRP12 in regulating lipid synthesis and secretion. In hepatoma cells and primary mouse hepatocytes, CTRP12 treatment inhibits triglyceride synthesis by suppressing glycerophosphate acyltransferase (GPAT) and diacylglycerol acyltransferase (DGAT) expression. CTRP12 treatment als...
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: Stefanie Y. Tan, Hannah C. Little, Dylan C. Sarver, Paul A. Watkins, G. William Wong Tags: RESEARCH LETTER Source Type: research

Coupled network of the circadian clocks: a driving force of rhythmic physiology
AbstractThe circadian system is composed of coupled endogenous oscillators that allow living beings, including humans, to anticipate and adapt to daily changes in their environment. In mammals, circadian clocks form a hierarchically organized network with a “master clock” located in the suprachiasmatic nucleus (SCN) of the hypothalamus, which ensures entrainment of subsidiary oscillators to environmental cycles. Robust rhythmicity of body clocks is indispensable for temporally coordinating organ functions, and the disruption or misalignment of circ adian rhythms caused for instance by modern lifestyle is strong...
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: Anna ‐Marie Finger, Charna Dibner, Achim Kramer Tags: REVIEW Source Type: research

Cleavage of OmpX by protealysin can regulate Serratia proteamaculans invasion
AbstractProtealysin is a thermolysin ‐like protease ofSerratia proteamaculans capable of specifically cleaving actin, which correlates with the invasive activity of these bacteria. Here, we show that inactivation of the protealysin gene does not inhibit invasion but, in contrast, leads to a twofold increase in theS. proteamaculans invasive activity. By mass spectrometry, we identified the outer membrane protein OmpX as a substrate of protealysin. RecombinantE. coli carrying the OmpX gene truncated by 40 N ‐terminal residues or both the OmpX and protealysin genes, in contrast to the full‐length OmpX, do not increase a...
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: Olga Tsaplina, Ilya Demidyuk, Tatiana Artamonova, Mikhail Khodorkovsky, Sofia Khaitlina Tags: RESEARCH ARTICLE Source Type: research

ΔNp63 is upregulated during salivary gland regeneration following duct ligation and irradiation in mice
AbstractThe transcription factor p63, a component of the p53 family, has important functions in development, homeostasis, and regeneration of epithelial tissues. However, the role of p63 in the regeneration of exocrine glands, including the salivary glands (SGs), has not been fully investigated. We investigated p63 expression in SG regeneration induced by duct ligation and irradiation. The expression of ΔNp63, a p63 isoform, increased and was co‐localized with Keratin5 (Krt5) during the regeneration process of SGs. Some ΔNp63‐ and Krt5‐positive cells were myoepithelial cells. Furthermore, ΔNp63 expr...
Source: FEBS Letters - August 4, 2020 Category: Biochemistry Authors: Kazuki Ikai, Manabu Sakai, Hitomi Minagi, Nao Gojo, Takayoshi Sakai Tags: RESEARCH LETTER Source Type: research

TOR kinase activity in Chlamydomonas  reinhardtii is modulated by cellular metabolic states
Target of rapamycin (TOR) kinase is a sensor and a central integrator of internal and external metabolic cues. However, in algae and in higher plants, the components of TOR kinase signaling are yet to be characterized. Here, we establish an assay system to study TOR kinase activity inChlamydomonas  reinhardtii using the phosphorylation status of its putative downstream target, CrS6K. Using this assay, we probe the modulation of cellular TOR kinase activity under various physiological states such as photoautotrophy, heterotrophy, mixotrophy, and nitrogen (N) starvation. Importantly, we uncover that excess acetate in th...
Source: FEBS Letters - August 3, 2020 Category: Biochemistry Authors: Shivani Upadhyaya, Shreya Agrawal, Anmol Gorakshakar, Basuthkar Jagadeeshwar Rao Tags: Research Article Source Type: research

The mysterious role of the NLRP9 pyrin domain in inflammasome assembly
FEBS Letters, EarlyView. (Source: FEBS Letters)
Source: FEBS Letters - July 31, 2020 Category: Biochemistry Authors: Eva Alba Tags: Commentary Source Type: research

Irc3 is a monomeric DNA branch point ‐binding helicase in mitochondria of the yeast Saccharomyces cerevisiae
AbstractIrc3 is a Superfamily II DNA helicase required for the maintenance of mitochondrial DNA stability inSaccharomyces cerevisiae. Here, we show that recombinant Irc3 is a monomeric protein and that it can form a binary complex with forked DNA. The catalytically active enzyme is a monomer as no positive cooperativity of ATP hydrolysis or DNA unwinding can be detected. Interestingly, we find that Irc3 prefers to unwind the nascent lagging strand at a replication fork. Using DNase I footprinting, we demonstrate that Irc3 captures DNA substrates by establishing a strong contact at the DNA branching point. Additional protec...
Source: FEBS Letters - July 31, 2020 Category: Biochemistry Authors: Vlad ‐Julian Piljukov, Natalja Garber, Tiina Sedman, Juhan Sedman Tags: RESEARCH ARTICLES Source Type: research

Creative communication – using poetry and games to generate dialogue between scientists and non‐scientists
AbstractScience communication is often perceived as an opportunity for scientists to broadcast their expertise and knowledge to a general public, so that this lay audience might better understand a specific field of study. However, in order for science to be made more accessible, science communication initiatives need to move away from this one ‐way discourse aimed at an unrealistically homogenous public. Instead science communicators need to consider how best to engage multiple audiences in a two‐way communication that can both inform research governance and lead to the co‐creation of new knowledge. In this article,...
Source: FEBS Letters - July 29, 2020 Category: Biochemistry Authors: Sam Illingworth Tags: THE SCIENTISTS ’ FORUM Source Type: research

Front Cover
Cover illustration The cover image is taken from the article “Synthetic biology principles for the design of protein with novel structures and functions” by W. Zhou et al. (pp.https://doi.org/10.1002/1873-3468.137962199 –2212). (Source: FEBS Letters)
Source: FEBS Letters - July 26, 2020 Category: Biochemistry Tags: Issue Information Source Type: research

Transcriptional shifts in delignification ‐defective mutants of the white‐rot fungus Pleurotus ostreatus
In this study, we conducted a comparative transcriptome analysis to identify genes that are differentially expressed in ligninolysis ‐deficient mutant strains. Putative ligninolytic genes that are highly expressed in parental strains are significantly downregulated in the mutant strains. On the contrary, many putative cellulolytic and xylanolytic genes are upregulated in thechd1 ‐1, Δpex1, and Δgat1 strains. Identifying transcriptional alterations in mutant strains could provide new insights into the regulatory mechanisms of lignocellulolytic genes inP. ostreatus. (Source: FEBS Letters)
Source: FEBS Letters - July 22, 2020 Category: Biochemistry Authors: Hongli Wu, Takehito Nakazawa, Atsuki Takenaka, Rina Kodera, Ryota Morimoto, Masahiro Sakamoto, Yoichi Honda Tags: RESEARCH ARTICLES Source Type: research

Coronin 2B regulates dendrite outgrowth by modulating actin dynamics
AbstractCytoskeletal remodeling is indispensable for the development and maintenance of neuronal structures and functions. However, the molecular machinery that controls the balance between actin polymerization and depolymerization during these processes is incompletely understood. Here, we report that Coronin 2B, a conserved actin ‐binding protein, is concentrated at the tips of developing dendrites and that knockdown of Coronin 2B inhibits the growth of dendrites. Importantly, Coronin 2B interacts with actin and reduces the F‐actin/G‐actin ratio. Furthermore, the coiled‐coil domain of Coronin 2B is required for i...
Source: FEBS Letters - July 21, 2020 Category: Biochemistry Authors: Yuewen Chen, Jinying Xu, Yulin Zhang, Shuangshuang Ma, Wanying Yi, Saijuan Liu, Xiaojun Yu, Jiali Wang, Yu Chen Tags: RESEARCH ARTICLES Source Type: research

Complement in sepsis —when science meets clinics
Sepsis as life ‐threatening organ dysfunction caused by microorganisms represents a dreadful challenge for the immune system. The role of the complement system as major column of innate immunity has been extensively studied in various sepsis models, but its translational value remains in the dark. Complement act ivation products, such as C3a and C5a, and their corresponding receptors provide useful diagnostic tools and promising targets to improve organ function and outcome. However, a monotherapeutic complement intervention irrespective of the current immune function seems insufficient to reverse the compl ex sepsis mec...
Source: FEBS Letters - July 21, 2020 Category: Biochemistry Authors: Tom E. Mollnes, Markus Huber ‐Lang Tags: Review Article Source Type: research

Coronin 2B regulates dendrite outgrowth by modulating actin dynamics
AbstractCytoskeletal remodeling is indispensable for the development and maintenance of neuronal structures and functions. However, the molecular machinery that controls the balance between actin polymerization and depolymerization during these processes is incompletely understood. Here, we report that Coronin 2B, a conserved actin ‐binding protein, is concentrated at the tips of developing dendrites and that knockdown of Coronin 2B inhibits the growth of dendrites. Importantly, Coronin 2B interacts with actin and reduces the F‐actin/G‐actin ratio. Furthermore, the coiled‐coil domain of Coronin 2B is required for i...
Source: FEBS Letters - July 21, 2020 Category: Biochemistry Authors: Yuewen Chen, Jinying Xu, Yulin Zhang, Shuangshuang Ma, Wanying Yi, Saijuan Liu, Xiaojun Yu, Jiali Wang, Yu Chen Tags: RESEARCH ARTICLES Source Type: research

Complement in sepsis —when science meets clinics
Sepsis as life ‐threatening organ dysfunction caused by microorganisms represents a dreadful challenge for the immune system. The role of the complement system as major column of innate immunity has been extensively studied in various sepsis models, but its translational value remains in the dark. Complement act ivation products, such as C3a and C5a, and their corresponding receptors provide useful diagnostic tools and promising targets to improve organ function and outcome. However, a monotherapeutic complement intervention irrespective of the current immune function seems insufficient to reverse the compl ex sepsis mec...
Source: FEBS Letters - July 21, 2020 Category: Biochemistry Authors: Tom E. Mollnes, Markus Huber ‐Lang Tags: Review Article Source Type: research

TOR kinase activity in Chlamydomonas reinhardtii is modulated by cellular metabolic states
AbstractTarget Of Rapamycin (TOR) kinase is a sensor as well as a central integrator of internal and external metabolic cues. However, in algae and in higher plants, the components of TOR kinase ‐signaling are yet to be characterized. Here, we establish an assay system to study TOR kinase activity inChlamydomonas reinhardtii using the phosphorylation status of its putative downstream target, CrS6K. Using this assay, we probe the modulation of cellular TOR kinase activity under various physiological states such as photoautotrophy, heterotrophy, mixotrophy and nitrogen (N) ‐starvation. Importantly, we uncover that excess...
Source: FEBS Letters - July 17, 2020 Category: Biochemistry Authors: Shivani Upadhyaya, Shreya Agrawal, Anmol Gorakshakar, Basuthkar Jagadeeshwar Rao Tags: RESEARCH ARTICLES Source Type: research

Phosphorylation of the N ‐terminal domain of ribosomal P‐stalk protein uL10 governs its association with the ribosome
AbstractThe uL10 ‐protein is the main constituent of the ribosomal‐P‐stalk, anchoring the whole stalk to the ribosome through interactions with rRNA. The P‐stalk is the core of the GTPase associated center (GAC), a critical element for ribosome biogenesis and ribosome translational activity. All P‐stalk pr oteins (uL10, P1 and P2) undergo phosphorylation within their C‐termini. Here, we show that uL10 has multiple phosphorylation sites, mapped also within the N‐terminal rRNA‐binding‐domain. Our results reveal that the introduction of a negative charge within the N‐terminus of uL10 impairs it s associati...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: Kamil Filipek, Barbara Michalec ‐Wawiórka, Aleksandra Boguszewska, Sebastian Kmiecik, Marek Tchórzewski Tags: ARTICLE Source Type: research

Retraction: Endothelial cell ‐specific deficiency of the adenosine deaminase ADAR1 aggravates LPS‐induced lung injury in mice via an MDA5‐independent pathway
Wang, X ‐L, Yan, R, Zhang, Z, Cong, G‐Z, Yi, Z‐J, Leng, Y‐P, and Chen AF, Endothelial cell‐specific deficiency of the adenosine deaminase ADAR1 aggravates LPS‐induced lung injury in mice via an MDA5‐independent pathway,FEBS Letters, 2020.https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.13754The above article, published online on February 12, 2020, in Wiley Online Library (wileyonlinelibrary.com) as Early View, has been retracted by agreement between the authors, the Journal Managing Editor Felix Wieland, and John Wiley& Sons Ltd. The retraction, requested by the authors, has been agreed due to c...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Tags: Retraction Source Type: research

Front Cover
Cover illustration Cover image by Foundry Co from Pixabay (https://pixabay.com/photos/rideferris-wheel-spin-rotate-839654/). (Source: FEBS Letters)
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Tags: Issue Information Source Type: research

Editorial
FEBS Letters, Volume 594, Issue 13, Page 2029-2030, July 2020. (Source: FEBS Letters)
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: Alexis R. Barr Tags: Editorial Source Type: research

Apolipoprotein L1 is transcriptionally regulated by SP1, IRF1 and IRF2 in hepatoma cells
AbstractApolipoprotein L1 (APOL1) participates in lipid metabolism. Here, we investigate the mechanisms regulatingAPOL1 gene expression in hepatoma cells. We demonstrate that the −80 nt to +31 nt region of theAPOL1 promoter, which contains one SP transcription factor ‐binding GT‐Box and an interferon regulatory factor (IRF)‐binding ISRE element, maintains the maximum activity. Mutation of the GT‐Box and IRSE element dramatically reducesAPOL1 promoter activity. EMSA and chromatin immunoprecipitation assays reveal that the transcription factors Sp1, IRF1, and IRF2 could interact with their cognate binding sites...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: De ‐Ping Wang, Zhao‐Xi Yu, Zong‐Cun He, Jin‐Fu Liao, Xue‐Bin Shen, Peng‐Li Zhu, Wan‐Nan Chen, Xu Lin, Shang‐Hua Xu Tags: RESEARCH ARTICLE Source Type: research

The golgin family exhibits a propensity to form condensates in living cells
AbstractThe Golgi is surrounded by a ribosome ‐excluding matrix. Recently, we reported that the cis‐Golgi‐localized golgin GM130 can phase‐separate to form dynamic, liquid‐like condensatesin vitro andin vivo. Here, we show that overexpression of each of the remaining cis (golgin160, GMAP210) and trans (golgin97, golgin245, GCC88, GCC185) golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB ‐SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed co...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: Pascal Ziltener, Aleksander A. Rebane, Morven Graham, Andreas M. Ernst, James E. Rothman Tags: RESEARCH LETTER Source Type: research

Phosphorylation of the N ‐terminal domain of ribosomal P‐stalk protein uL10 governs its association with the ribosome
AbstractThe uL10 ‐protein is the main constituent of the ribosomal‐P‐stalk, anchoring the whole stalk to the ribosome through interactions with rRNA. The P‐stalk is the core of the GTPase associated center (GAC), a critical element for ribosome biogenesis and ribosome translational activity. All P‐stalk pr oteins (uL10, P1 and P2) undergo phosphorylation within their C‐termini. Here, we show that uL10 has multiple phosphorylation sites, mapped also within the N‐terminal rRNA‐binding‐domain. Our results reveal that the introduction of a negative charge within the N‐terminus of uL10 impairs it s associati...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: Kamil Filipek, Barbara Michalec ‐Wawiórka, Aleksandra Boguszewska, Sebastian Kmiecik, Marek Tchórzewski Tags: ARTICLE Source Type: research

Retraction: Endothelial cell ‐specific deficiency of the adenosine deaminase ADAR1 aggravates LPS‐induced lung injury in mice via an MDA5‐independent pathway
Wang, X ‐L, Yan, R, Zhang, Z, Cong, G‐Z, Yi, Z‐J, Leng, Y‐P, and Chen AF, Endothelial cell‐specific deficiency of the adenosine deaminase ADAR1 aggravates LPS‐induced lung injury in mice via an MDA5‐independent pathway,FEBS Letters, 2020.https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.13754The above article, published online on February 12, 2020, in Wiley Online Library (wileyonlinelibrary.com) as Early View, has been retracted by agreement between the authors, the Journal Managing Editor Felix Wieland, and John Wiley& Sons Ltd. The retraction, requested by the authors, has been agreed due to c...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Tags: Retraction Source Type: research

Front Cover
Cover illustration Cover image by Foundry Co from Pixabay (https://pixabay.com/photos/rideferris-wheel-spin-rotate-839654/). (Source: FEBS Letters)
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Tags: Issue Information Source Type: research

Editorial
FEBS Letters, Volume 594, Issue 13, Page 2029-2030, July 2020. (Source: FEBS Letters)
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: Alexis R. Barr Tags: Editorial Source Type: research

The golgin family exhibits a propensity to form condensates in living cells
AbstractThe Golgi is surrounded by a ribosome ‐excluding matrix. Recently, we reported that the cis‐Golgi‐localized golgin GM130 can phase‐separate to form dynamic, liquid‐like condensatesin vitro andin vivo. Here, we show that overexpression of each of the remaining cis (golgin160, GMAP210) and trans (golgin97, golgin245, GCC88, GCC185) golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB ‐SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed co...
Source: FEBS Letters - July 15, 2020 Category: Biochemistry Authors: Pascal Ziltener, Aleksander A. Rebane, Morven Graham, Andreas M. Ernst, James E. Rothman Tags: RESEARCH LETTER Source Type: research

Co ‐expression with chaperones can affect protein 3D structure as exemplified by loss‐of‐function variants of human prolidase
Prolidase catalyzes the cleavage of dipeptides containing proline on their C terminus. The reduction in prolidase activity is the cause of a rare disease named 'Prolidase Deficiency'. Local structural disorder was indicated as one of the causes for diminished prolidase activity. Previous studies showed that heat shock proteins can partially recover prolidase activityin  vivo. To analyze this mechanism of enzymatic activity rescue, we compared the crystal structures of selected prolidase mutants expressed in the absence and in the presence of chaperones. Our results confirm that protein chaperones facilitate the format...
Source: FEBS Letters - July 14, 2020 Category: Biochemistry Authors: El żbieta Wątor, Maria Rutkiewicz, Manfred S. Weiss, Piotr Wilk Tags: Research Letter Source Type: research

Organization of gangliosides into membrane nanodomains
Gangliosides are glycosphingolipids consisting of a ceramide base and a bulky sugar chain that contains one or more sialic acids. This unique structure endows gangliosides with a strong tendency to self ‐aggregate in solution, as well as in cellular membranes, where they can form nanoscopic assemblies called ganglioside nanodomains. As gangliosides are important biological molecules involved in a number of physiological processes, characterization of their lateral organization in membranes is ess ential. This review aims at providing comprehensive information about the nanoscale organization of gangliosides in various sy...
Source: FEBS Letters - July 10, 2020 Category: Biochemistry Authors: Maria J. Sarmento, Joana C. Ricardo, Mariana Amaro, Radek Šachl Tags: Review Article Source Type: research

Crystal structure of the usher chaperone YadV reveals a monomer with the proline lock in closed conformation suggestive of an intermediate state
AbstractCell surface pili assembled by the chaperone ‐usher (CU) pathway play a crucial role in the adhesion of uropathogenicEscherichia coli. YadV is the chaperone component of the CU pathway of Yad pili. Here, we report the crystal structure of YadV fromE. coli. In contrast to major usher chaperones, YadV is a monomer in solution as well as in the crystallographic symmetry, and the monomeric form is a preferred state for interacting with pilus subunits. Moreover, we observed a closed conformation for the proline lock, a crucial structural element for chaperone –pilus subunit interaction. MD simulation shows that ...
Source: FEBS Letters - July 10, 2020 Category: Biochemistry Authors: Nishant Kumar Pandey, Garima Verma, Gajraj Singh Kushwaha, Mrutyunjay Suar, Neel Sarovar Bhavesh Tags: RESEARCH LETTER Source Type: research

Organization of gangliosides into membrane nanodomains
Gangliosides are glycosphingolipids consisting of a ceramide base and a bulky sugar chain that contains one or more sialic acids. This unique structure endows gangliosides with a strong tendency to self ‐aggregate in solution, as well as in cellular membranes, where they can form nanoscopic assemblies called ganglioside nanodomains. As gangliosides are important biological molecules involved in a number of physiological processes, characterization of their lateral organization in membranes is ess ential. This review aims at providing comprehensive information about the nanoscale organization of gangliosides in various sy...
Source: FEBS Letters - July 10, 2020 Category: Biochemistry Authors: Maria J. Sarmento, Joana C. Ricardo, Mariana Amaro, Radek Šachl Tags: Review Article Source Type: research

Crystal structure of the usher chaperone YadV reveals a monomer with the proline lock in closed conformation suggestive of an intermediate state
AbstractCell surface pili assembled by the chaperone ‐usher (CU) pathway play a crucial role in the adhesion of uropathogenicEscherichia coli. YadV is the chaperone component of the CU pathway of Yad pili. Here, we report the crystal structure of YadV fromE. coli. In contrast to major usher chaperones, YadV is a monomer in solution as well as in the crystallographic symmetry, and the monomeric form is a preferred state for interacting with pilus subunits. Moreover, we observed a closed conformation for the proline lock, a crucial structural element for chaperone –pilus subunit interaction. MD simulation shows that ...
Source: FEBS Letters - July 10, 2020 Category: Biochemistry Authors: Nishant Kumar Pandey, Garima Verma, Gajraj Singh Kushwaha, Mrutyunjay Suar, Neel Sarovar Bhavesh Tags: RESEARCH LETTER Source Type: research

TRIP suppresses cell proliferation and invasion in choroidal melanoma via promoting the proteasomal degradation of Twist1
AbstractChoroidal melanoma (CM) remains the most prevalent form of intraocular malignancy, and the prognosis of affected patients is poor. While the E3 ubiquitin ligase TRIP is known to play key regulatory roles in multiple diseases, its relevance in CM remains uncertain. In the present study, we found that TRIP overexpression is sufficient to inhibit the proliferation, invasion, and EMT of CM cellsin vitro, whereas the opposite phenotypes are observed following TRIP knockdown. We further determined that TRIP is able to promote the K48 ‐polyubiquitination of EMT‐associated transcription factor Twist1, thereby suppressi...
Source: FEBS Letters - July 8, 2020 Category: Biochemistry Authors: Chao Wei, Xiaofei Zhao, Lei Wang, Han Zhang Tags: RESEARCH ARTICLES Source Type: research

Complement in Sepsis ‐ when Science meets Clinics
AbstractSepsis as life ‐threatening organ dysfunction caused by microorganisms represents a dreadful challenge for the immune system. The role of the complement system as major column of innate immunity has been extensively studied in various sepsis models but its translational value remains in the dark. Complement acti vation products, such as C3a and C5a, and their corresponding receptors provide useful diagnostic tools and promising targets to improve organ function and outcome. However, a mono‐therapeutic complement intervention irrespective of the current immune function seems insufficient to reverse the com plex ...
Source: FEBS Letters - July 4, 2020 Category: Biochemistry Authors: Tom E. Mollnes, Markus Huber ‐Lang Tags: REVIEW Source Type: research

Glycolysis, via NADH ‐dependent dimerisation of CtBPs, regulates hypoxia‐induced expression of CAIX and stem‐like breast cancer cell survival
ABSTRACTAdaptive responses to hypoxia are mediated by the hypoxia ‐inducible factor (HIF) family of transcription factors. These responses include the upregulation of glycolysis to maintain ATP production. This also generates acidic metabolites, which require HIF‐induced carbonic anhydrase IX (CAIX) for their neutralisation. C‐terminal binding proteins (CtBP s) are co‐regulators of gene transcription and couple glycolysis with gene transcription due to their regulation by the glycolytic coenzyme NADH. Here, we find that experimental manipulation of glycolysis and CtBP function in breast cancer cells through multipl...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Mira Kreuzer, A. Banerjee, Charles N. Birts, M. Darley, A. Tavassoli, M. Ivan, Jeremy P. Blaydes Tags: RESEARCH LETTER Source Type: research

SHP ‐1 ameliorates nonalcoholic steatohepatitis by inhibiting proinflammatory cytokine production
AbstractInflammation is the main contributor for the pathogenesis of nonalcoholic steatohepatitis (NASH). Src homology region 2 domain ‐containing phosphatase 1 (SHP‐1, also known as PTPN6) is regarded as a negative regulator of inflammation, but its role in NASH remains unknown. Here, hepatocyte‐specific Ptpn6 knockout mice (Ptpn6HKO) and adenovirus vector ‐mediated ectopic expression of SHP‐1 (AdSHP1) were used to evaluate the role of SHP‐1 in a methionine‐ and choline‐deficient (MCD) diet‐induced NASH model. Compared with the control littermates,Ptpn6HKO mice show exacerbated hepatic steatosis, inflamm...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Lin Lin, Jie Jian, Chun ‐Yan Song, Fei Chen, Kai Ding, Wei‐Fen Xie, Ping‐Fang Hu Tags: RESEARCH ARTICLES Source Type: research

Small molecule 3PO inhibits glycolysis but does not bind to 6 ‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase‐3 (PFKFB3)
Abstract6 ‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase isoform 3 (PFKFB3) is a key enzyme of the glycolytic pathway, and it plays an essential role in angiogenesis. 3‐(3‐pyridinyl)‐1‐(4‐pyridinyl)‐2‐propen‐1‐one (3PO) is frequently used as a glycolysis inhibitor and is thoug ht to inhibit PFKFB3. However, this latter effect of 3PO has never been investigated in detail and was the aim of the present study. To demonstrate binding of 3PO to PFKFB3, we used isothermal titration calorimetry. However, 3PO did not bind to PFKFB3, even up to 750 µM, in contrast to 3 µM of AZ6 7, which...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Besa Emini Veseli, Paola Perrotta, Pieter Van Wielendaele, Anne ‐Marie Lambeir, Anahita Abdali, Stefano Bellosta, Giovanni Monaco, Geert Bultynck, Wim Martinet, Guido De Meyer Tags: COMMUNICATION Source Type: research

The D1:Ser268 residue of Photosystem II contributes to an alternative pathway for QB protonation in the absence of bound bicarbonate
AbstractPhotosystem II catalyses the splitting of water and the reduction of plastoquinone in thylakoid membranes of all oxygenic photosynthetic organisms. The final step in quinol formation is protonation of the reduced secondary quinone electron acceptor QB2 ‐(H+) to give QBH2. The proton for this step is hypothesized to be provided by a hydrogen ‐bond network incorporating amino acids from the Photosystem II D1 and D2 reaction center proteins, together with several bound waters and a bicarbonate ion ligated to a non‐heme iron found between the primary plastoquinone electron acceptor QA and QB. The aim of this...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Jack A. Forsman, Julian J. Eaton ‐Rye Tags: RESEARCH ARTICLES Source Type: research

Glycolysis, via NADH ‐dependent dimerisation of CtBPs, regulates hypoxia‐induced expression of CAIX and stem‐like breast cancer cell survival
ABSTRACTAdaptive responses to hypoxia are mediated by the hypoxia ‐inducible factor (HIF) family of transcription factors. These responses include the upregulation of glycolysis to maintain ATP production. This also generates acidic metabolites, which require HIF‐induced carbonic anhydrase IX (CAIX) for their neutralisation. C‐terminal binding proteins (CtBP s) are co‐regulators of gene transcription and couple glycolysis with gene transcription due to their regulation by the glycolytic coenzyme NADH. Here, we find that experimental manipulation of glycolysis and CtBP function in breast cancer cells through multipl...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Mira Kreuzer, A. Banerjee, Charles N. Birts, M. Darley, A. Tavassoli, M. Ivan, Jeremy P. Blaydes Tags: RESEARCH LETTER Source Type: research

SHP ‐1 ameliorates nonalcoholic steatohepatitis by inhibiting proinflammatory cytokine production
AbstractInflammation is the main contributor for the pathogenesis of nonalcoholic steatohepatitis (NASH). Src homology region 2 domain ‐containing phosphatase 1 (SHP‐1, also known as PTPN6) is regarded as a negative regulator of inflammation, but its role in NASH remains unknown. Here, hepatocyte‐specific Ptpn6 knockout mice (Ptpn6HKO) and adenovirus vector ‐mediated ectopic expression of SHP‐1 (AdSHP1) were used to evaluate the role of SHP‐1 in a methionine‐ and choline‐deficient (MCD) diet‐induced NASH model. Compared with the control littermates,Ptpn6HKO mice show exacerbated hepatic steatosis, inflamm...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Lin Lin, Jie Jian, Chun ‐Yan Song, Fei Chen, Kai Ding, Wei‐Fen Xie, Ping‐Fang Hu Tags: RESEARCH ARTICLES Source Type: research

Small molecule 3PO inhibits glycolysis but does not bind to 6 ‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase‐3 (PFKFB3)
Abstract6 ‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase isoform 3 (PFKFB3) is a key enzyme of the glycolytic pathway, and it plays an essential role in angiogenesis. 3‐(3‐pyridinyl)‐1‐(4‐pyridinyl)‐2‐propen‐1‐one (3PO) is frequently used as a glycolysis inhibitor and is thoug ht to inhibit PFKFB3. However, this latter effect of 3PO has never been investigated in detail and was the aim of the present study. To demonstrate binding of 3PO to PFKFB3, we used isothermal titration calorimetry. However, 3PO did not bind to PFKFB3, even up to 750 µM, in contrast to 3 µM of AZ6 7, which...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Besa Emini Veseli, Paola Perrotta, Pieter Van Wielendaele, Anne ‐Marie Lambeir, Anahita Abdali, Stefano Bellosta, Giovanni Monaco, Geert Bultynck, Wim Martinet, Guido De Meyer Tags: COMMUNICATION Source Type: research

The D1:Ser268 residue of Photosystem II contributes to an alternative pathway for QB protonation in the absence of bound bicarbonate
AbstractPhotosystem II catalyses the splitting of water and the reduction of plastoquinone in thylakoid membranes of all oxygenic photosynthetic organisms. The final step in quinol formation is protonation of the reduced secondary quinone electron acceptor QB2 ‐(H+) to give QBH2. The proton for this step is hypothesized to be provided by a hydrogen ‐bond network incorporating amino acids from the Photosystem II D1 and D2 reaction center proteins, together with several bound waters and a bicarbonate ion ligated to a non‐heme iron found between the primary plastoquinone electron acceptor QA and QB. The aim of this...
Source: FEBS Letters - July 3, 2020 Category: Biochemistry Authors: Jack A. Forsman, Julian J. Eaton ‐Rye Tags: RESEARCH ARTICLES Source Type: research

Crystal structure of TEX101, a glycoprotein essential for male fertility, reveals the presence of tandemly arranged Ly6/uPAR domains
AbstractTestis ‐expressed gene 101 (TEX101) is a glycosyl‐phosphatidylinositol (GPI)‐anchored glycoprotein essential for sperm fertility and spermatogenesis. TEX101 interacts with lymphocyte antigen 6 complex, locus K (Ly6k) as well as a disintegrin and metallopeptidase domain 3 (ADAM3). Although these prote ins are considered essential for fertility, the associated mechanisms remain uncharacterized. Herein, we determined the crystal structure of human and mouse TEX101, revealing that TEX101 contains two tandem Ly6/uPAR (LU) domains. Detailed structural analyses revealed characteristic surfaces of TEX10 1 that may be...
Source: FEBS Letters - July 1, 2020 Category: Biochemistry Authors: Mamiko Masutani, Shunya Sakurai, Toshiyuki Shimizu, Umeharu Ohto Tags: RESEARCH LETTER Source Type: research