Phosphorylation of TG ‐interacting factor 1 at carboxyl‐terminal sites in response to insulin regulates adipocyte differentiation
TG-interacting factor 1 (TGIF1) contributes to the differentiation of white preadipocytes; however, its regulation is not well elucidated. We highlight that the insulin-induced ERK activation phosphorylates the T235 or T239 residue of TGIF1, which is crucial for the promotion of mitotic clonal expansion and adipocyte differentiation. TG-interacting factor 1 (TGIF1) contributes to the differentiation of murine white preadipocyte and human adipose tissue-derived stem cells; however, its regulation is not well elucidated. Insulin is a component of the adipogenic cocktail that induces ERK signaling. TGIF1 phosphorylation and s...
Source: FEBS Letters - March 13, 2024 Category: Biochemistry Authors: Yu ‐Hao Chang,
Yu‐Hua Tseng,
Ju‐Ming Wang,
Yau‐Sheng Tsai,
Xin‐Lei Liu,
Huei‐Sheng Huang Tags: Research Letter Source Type: research
Redox imbalance and metabolic defects in the context of Alzheimer disease
During aging, a progressive failure of energy metabolism occurs resulting in brain hypometabolism. This condition, combined with redox disturbance, contributes to increase neuronal cell vulnerability ultimately leading to neurodegeneration. Down syndrome and Alzheimer disease neuropathologies present several molecular similarities, among which perturbation of redox homeostasis and reduced energy production are major players that accelerate neuronal damage. Redox reactions play a critical role for intracellular processes, including pathways involved in metabolism and signaling. Reactive oxygen species (ROS) act either as se...
Source: FEBS Letters - March 13, 2024 Category: Biochemistry Authors: Fabio Di Domenico,
Chiara Lanzillotta,
Marzia Perluigi Tags: Review Source Type: research
Cdc73 is a major regulator of apoptosis ‐inducing factor 1 expression in Saccharomyces cerevisiae via H3K36 methylation
In this report, we show the time-dependent induction ofAIF1 under hydrogen peroxide stress. Additionally, we find thatAIF1 expression in response to hydrogen peroxide is mediated by two transcription factors, Yap5 (DNA binding) and Cdc73 (non-DNA binding). Furthermore, substituting the H3K36 residue with another amino acid significantly abrogatesAIF1 expression. However, substituting the lysine (K) in H3K4 or H3K79 with alanine (A) does not affectAIF1 expression level under hydrogen peroxide stress. Altogether, reducedAIF1 expression incdc73 Δ is plausibly due to reduced H3K36me3 levels in the cells. (Source: FEBS Letters)
Source: FEBS Letters - March 12, 2024 Category: Biochemistry Authors: Nitu Saha,
Santoshi Acharjee,
Raghuvir Singh Tomar Tags: Research Article Source Type: research
Front Cover
Cover illustration Ribosome engaged in translation. Image: Christoph Burgstedt/Shutterstock.com. The image refers to Nurullinaet al. ‘Cryo-EM structure of the inactive ribosome complex accumulated in chick embryo cells in cold-stress conditions.’ (Source: FEBS Letters)
Source: FEBS Letters - March 11, 2024 Category: Biochemistry Tags: Issue Information Source Type: research
How enzyme ‐centered approaches are advancing research on cyclic oligo‐nucleotides
Cyclic nucleotides serve as second messengers throughout kingdoms of life and regulate various pathways. Here, we review recent milestones in cyclic nucleotide biology, focusing on different enzyme folds that synthesize these signals, their regulatory mechanisms, and pleiotropic downstream signaling events. Our particular focus is on enzyme-centered approaches specifically targeting nucleotidyltransferases, which have enabled the discovery of novel cyclic nucleotides. Cyclic nucleotides are the most diversified category of second messengers and are found in all organisms modulating diverse pathways. While cAMP and cGMP hav...
Source: FEBS Letters - March 8, 2024 Category: Biochemistry Authors: Simon J. Wenzl,
Carina C. de Oliveira Mann Tags: Perspective Source Type: research
TERRA and the alternative lengthening of telomeres: a dangerous affair
The long noncoding RNA TERRA controls telomere length homeostasis in human cancers with an activated alternative lengthening of telomeres (ALT) mechanism. Telomeric R-loops formed between TERRA and telomeric DNA (telR-loops) promote telomere elongation through homology-directed repair. However, if not properly regulated, TERRA and telR-loops can lead to rapid cleavage of telomeric DNA and telomere loss. Eukaryotic telomeres are transcribed into the long noncoding RNA TERRA. A fraction of TERRA remains associated with telomeres by forming RNA:DNA hybrids dubbed telR-loops. TERRA and telR-loops are essential to promote telom...
Source: FEBS Letters - March 7, 2024 Category: Biochemistry Authors: Claus M. Azzalin Tags: In a Nutshell Source Type: research
Recruitment of ubiquitin E2 enzymes is determined jointly by the U ‐box domains and substrates of E3 ligases
This study reveals the influence of substrates and U-box domains on E2 recruitment, providing a novel perspective on the function of U-box domains of E3 ligases. (Source: FEBS Letters)
Source: FEBS Letters - March 5, 2024 Category: Biochemistry Authors: Bo Jin,
Bei Li,
Junyao Qu,
Yiheng Sun,
Mengran Wang,
Changjiang Yang,
Yuchen Fan,
Yanan Wang,
Peng Xu,
Haiying Sun,
Bo Jiang,
Bo Zhao Tags: Research Letter Source Type: research
Uncovering domain motif interactions using high ‐throughput protein–protein interaction detection methods
Protein –protein interactions (PPIs), often mediated by short linear motifs (SLiMs), shape cellular functions. This review provides an overview of SLiMs, and scrutinises current PPI detection techniques, highlighting their relevance to SLiM-mediated interactions and addressing challenges in detecting doma in–motif interactions (DMIs). Case studies, like BioGrid database analysis, suggest high-throughput PPI methods as reliable sources for predicting DMIs, enriching our understanding of cellular dynamics. Protein –protein interactions (PPIs) are often mediated by short linear motifs (SLiMs) in one protein and domain i...
Source: FEBS Letters - March 5, 2024 Category: Biochemistry Authors: Sobia Idrees,
Keshav Raj Paudel,
Tayyaba Sadaf,
Philip M. Hansbro Tags: Review Source Type: research
A life ‐time of hematopoietic cell function: ascent, stability, and decline
Here we review the aging of the hematopoietic system from its earliest stages in the embryo through fetal and adult life. We focus on the waves of hematopoietic cell generation during embryonic life and how cells of varying lineages, functions and life spans contribute to the normal development of the adult blood system and its progressive gain/loss of function in the aged adult. Aging is a set of complex processes that occur temporally and continuously. It is generally a unidirectional progression of cellular and molecular changes occurring during the life stages of cells, tissues and ultimately the whole organism. In ver...
Source: FEBS Letters - March 5, 2024 Category: Biochemistry Authors: Anna Popravko,
Lorna Mackintosh,
Elaine Dzierzak Tags: Review Source Type: research
