Effects of sound energy on proteins and their complexes
Irradiation of aqueous media with ultrasound produces combined chemical, mechanical, and thermal effects that are capable to affect proteins at all organization levels, from their primary to tertiary structure. Here we summarize the progress made in understanding the structure and behavior-modifying impact of ultrasound on proteins focusing on their assembly/disassembly and discuss the effect of ultrasound on specific protein functionality. Mechanical energy in the form of ultrasound, and protein complexes intuitively have been considered as two distinct unrelated topics. However, in the past few years, increasingly more a...
Source: FEBS Letters - October 15, 2023 Category: Biochemistry Authors: Anna Kozell,
Aleksei Solomonov,
Ulyana Shimanovich Tags: Review Source Type: research
Location of the photosynthetic carbon metabolism in microcompartments and separated phases in microalgal cells
Unicellular photosynthetic cells are composed of an ensemble of partitions, also named microcompartments, that contribute to the regulation of carbon acquisition, assimilation and storage. This perspective Review proposes to consider the distinct physico-chemical properties of each microcompartments when considering the metabolic regulation. Reversibly, the activation of specific metabolic pathway modulates the physico-chemical properties of specific microcompartments. Carbon acquisition, assimilation and storage in eukaryotic microalgae and cyanobacteria occur in multiple compartments that have been characterised by the l...
Source: FEBS Letters - October 13, 2023 Category: Biochemistry Authors: H élène Launay,
Luisana Avilan,
Cassy Gérard,
Goetz Parsiegla,
Véronique Receveur‐Brechot,
Brigitte Gontero,
Frédéric Carriere Tags: Perspective Source Type: research
Retraction statement: miR ‐518b is down‐regulated, and involved in cell proliferation and invasion by targeting Rap1b in esophageal squamous cell carcinoma.
(Source: FEBS Letters)
Source: FEBS Letters - October 12, 2023 Category: Biochemistry Tags: Retraction Notice Source Type: research
Location of the photosynthetic carbon metabolism in microcompartments and separated phases in microalgal cells
Unicellular photosynthetic cells are composed of an ensemble of partitions, also named microcompartments, that contribute to the regulation of carbon acquisition, assimilation and storage. This perspective Review proposes to consider the distinct physico-chemical properties of each microcompartments when considering the metabolic regulation. Reversibly, the activation of specific metabolic pathway modulates the physico-chemical properties of specific microcompartments. Carbon acquisition, assimilation and storage in eukaryotic microalgae and cyanobacteria occur in multiple compartments that have been characterised by the l...
Source: FEBS Letters - October 12, 2023 Category: Biochemistry Authors: H élène Launay,
Luisana Avilan,
Cassy Gérard,
Goetz Parsiegla,
Véronique Receveur‐Brechot,
Brigitte Gontero,
Frédéric Carriere Tags: Perspective Source Type: research
Scaffold, mechanics and functions of nuclear lamins
Nuclear lamins, constituents of the nuclear lamina, bridge the gap between the nuclear membranes and chromatin. Here we discuss the lamins ’ structure, assembly dynamics and their interactions with chromatin. We focus on the mechanical roles of lamins, from molecular to network scales, and finalize by linking the structural, mechanical, and molecular properties of lamins with biological function and disease. Nuclear lamins are type-V intermediate filaments that are involved in many nuclear processes. In mammals, A- and B-type lamins assemble into separate physical meshwork underneath the inner nuclear membrane, the nucle...
Source: FEBS Letters - October 10, 2023 Category: Biochemistry Authors: Amnon Buxboim,
Rafael Kronenberg ‐Tenga,
Sarka Salajkova,
Nili Avidan,
Hen Shahak,
Alice Thurston,
Ohad Medalia Tags: Review Source Type: research
Nutrient ‐dependent signaling pathways that control autophagy in yeast
Living organisms experience diverse external environments throughout life; one common situation is nutrient limitation. To survive nutrient stress, eukaryotic cells utilize macroautophagy/autophagy. During autophagy, cells remove unwanted material by packaging them within double-membraned autophagosomes and delivering the cargo to vacuoles for degradation. Here, we summarize different nutrient contexts and signaling pathways that regulate autophagy, specifically inSaccharomyces cerevisiae. Macroautophagy/autophagy is a highly conserved catabolic process vital for cellular stress responses and maintaining equilibrium wit...
Source: FEBS Letters - October 9, 2023 Category: Biochemistry Authors: Shree Padma Metur,
Daniel J. Klionsky Tags: Review Source Type: research
A new approach to making scientific research more efficient – rethinking sustainability
As interest in sustainability grows, many researchers raise questions about changing scientific practices. To enable effective change, we reconceptualize sustainability as an approach that optimizes the efficiency of procedures, thereby benefiting scientists and minimizing environmental footprints. Since the implementation of sustainable approaches can be challenging, we describe the 6R concept as a framework to arrive at actionable steps. (Source: FEBS Letters)
Source: FEBS Letters - October 9, 2023 Category: Biochemistry Authors: Patrick Penndorf,
Johannes Jabs Tags: The Scientists' Forum Source Type: research
Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses
Grimontia hollisae collagenase (Ghcol) consists of 767 amino acid residues with a single catalytic domain containing the zinc-binding motif H492EYVH496. The crystal structure of Ghcol in complex with its substrate (Gly-Pro-hydroxyproline-Gly-Pro-hydroxyproline) and site-directed mutagenesis of active-site Tyr residues revealed the catalytic mechanism: Glu493 functions as an acid and base catalyst while Tyr564 stabilizes the tetrahedral complex in the transition state. Grimontia hollisae collagenase (Ghcol) exhibits high collagen-degrading activity. To explore its catalytic mechanism, its substrate (Gly-Pro-Hyp-Gly-Pro-Hyp,...
Source: FEBS Letters - October 9, 2023 Category: Biochemistry Authors: Saori Ueshima,
Mizuki Yasumoto,
Yuto Kitagawa,
Kaho Akazawa,
Teisuke Takita,
Keisuke Tanaka,
Shunji Hattori,
Kimihiko Mizutani,
Bunzo Mikami,
Kiyoshi Yasukawa Tags: Research Letter Source Type: research
STAP ‐2 negatively regulates BCR‐mediated B cell activation by recruiting tyrosine‐protein kinase CSK to LYN
In this study, the role of STAP-2 in B cell functions was analyzed. BCR-mediated B cell activation was enhanced in STAP-2 KO mice by inhibiting recruitment of Csk to Lyn. In accordance with the results, antibody production was significantly higher in STAP-2 KO mice compared with WT mice. Therefore, STAP-2 is important for the regulation of BCR signaling in B cells. Although signal-transducing adaptor protein-2 (STAP-2) acts in certain immune responses, its role in B cell receptor (BCR)-mediated signals remains unknown. In this study, we have revealed that BCR-mediated signals, cytokine production and antibody production we...
Source: FEBS Letters - October 9, 2023 Category: Biochemistry Authors: Jun ‐ichi Kashiwakura,
Shoya Kawahara,
Iori Inagaki,
Kyosuke Inui,
Kodai Saitoh,
Kota Kagohashi,
Yuto Sasaki,
Fuki Kobayashi,
Yuichi Kitai,
Ryuta Muromoto,
Kenji Oritani,
Tadashi Matsuda Tags: Research Letter Source Type: research
Front Cover
Cover illustration By optimising the efficiency of experimental procedures, we can minimise the lab ’s environmental footprint, making research more sustainable. Read more in Penndorf and Jabs ‘A new approach to making scientific research more efficient ’ rethinking sustainability’ in this issue. (Source: FEBS Letters)
Source: FEBS Letters - October 9, 2023 Category: Biochemistry Tags: Issue Information Source Type: research
A new approach to making scientific research more efficient – rethinking sustainability
As interest in sustainability grows, many researchers raise questions about changing scientific practices. To enable effective change, we reconceptualize sustainability as an approach that optimizes the efficiency of procedures, thereby benefiting scientists and minimizing environmental footprints. Since the implementation of sustainable approaches can be challenging, we describe the 6R concept as a framework to arrive at actionable steps. (Source: FEBS Letters)
Source: FEBS Letters - October 9, 2023 Category: Biochemistry Authors: Patrick Penndorf,
Johannes Jabs Tags: The Scientists' Forum Source Type: research
