Front Cover
Cover illustration Human U2-OS cells stably expressing EGFP-LC3B (green) were grown in fed conditions, fixed and stained with anti-p62/AF 547 antibodies (red) and DAPI (blue). A representative confocal laser-scanning microscopy image is shown. Courtesy of Carmen Julia Pastor-Maldonade, Proikas-Cezanne laboratory, Eberhard Karls University Tuebingen, Germany. (Source: FEBS Letters)
Source: FEBS Letters - January 11, 2024 Category: Biochemistry Tags: Issue Information Source Type: research
The journey towards clinical adoption of MALDI ‐MS‐based imaging proteomics: from current challenges to future expectations
In recent years, there is increasing evidence to suggest that matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) technology could offer support in clinical decision-making processes. Here, we discuss some of the recent technical developments underpinning this progress and outline some of the more promising clinical proteomics applications which have been developed with a clear goal towards implementation in mind. Among the spatial omics techniques available, mass spectrometry imaging (MSI) represents one of the most promising owing to its capability to map the distribution of hundreds of pept...
Source: FEBS Letters - January 5, 2024 Category: Biochemistry Authors: Isabella Piga,
Fulvio Magni,
Andrew Smith Tags: Review Source Type: research
Molecular mechanisms of perilipin protein function in lipid droplet metabolism
Lipid droplets (LDs) are important for the storage of energy and lipid components. The perilipin family in mammals includes five proteins (PLIN1 to PLIN5), all of which are abundant on the surface of LDs in different tissues and play a role in LD stability and degradation by lipases. We discuss how perilipins interact with LDs, their function, and their implication in metabolic disease. Perilipins are abundant lipid droplet (LD) proteins present in all metazoans and also in Amoebozoa and fungi. Humans express five perilipins, which share a similar domain organization: an amino-terminal PAT domain and an 11-mer repeat regio...
Source: FEBS Letters - January 2, 2024 Category: Biochemistry Authors: Elena Griseti,
Abdoul Akim Bello,
Eric Bieth,
Bayane Sabbagh,
Jason S. Iacovoni,
Jo ëlle Bigay,
Henrik Laurell,
Alenka Čopič Tags: Review Source Type: research
Structural determinants of phosphorylation ‐dependent nuclear transport of HCMV DNA polymerase processivity factor UL44
The cell nucleus is separated from the cytoplasm by a double membrane, and transport of molecules occurs through aqueous channels called nuclear pore complexes. Here, we combined quantitative binding assays and X-ray crystallography to study the mechanistic details of how phosphorylation can influence the interaction of a protein with the nuclear transporter importin alpha, thereby regulating the process of nuclear import. Human cytomegalovirus (HCMV) DNA polymerase processivity factor UL44 is transported into the nucleus by importin (IMP) α/β through a classical nuclear localization signal (NLS), and this region is susc...
Source: FEBS Letters - December 30, 2023 Category: Biochemistry Authors: Emily M. Cross,
Oriano Marin,
Daryl Ariawan,
David Arag ão,
Giorgio Cozza,
Enzo Di Iorio,
Jade K. Forwood,
Gualtiero Alvisi Tags: Research Letter Source Type: research
Redox and metabolic reprogramming in breast cancer and cancer ‐associated adipose tissue
This review focuses on deciphering the redox and metabolic profiles of breast cancer and associated adipose tissue as a part of a unique Warburg pseudo-organ. In the light of the coupled cancer and adipose tissue redox-metabolic reprogramming, mechanistic links to estrogen receptors, tumor microenvironment cell heterogeneity, inflammation, and circadian rhythms as important players affecting breast cancer development, progression, and prognosis are also discussed (Created withBioRender.com). Redox and metabolic processes are tightly coupled in both physiological and pathological conditions. In cancer, their integration occ...
Source: FEBS Letters - December 29, 2023 Category: Biochemistry Authors: Tamara Zakic,
Vanja Pekovic ‐Vaughan,
Aleksandra Cvoro,
Aleksandra Korac,
Aleksandra Jankovic,
Bato Korac Tags: Review Source Type: research
Membranes that make fat: roles of membrane lipids as acyl donors for triglyceride synthesis and organelle function
Certain eukaryotes can synthesize triacylglycerol using an acyl-CoA-independent pathway. This activity is mediated by phospholipid diacylglycerol acyl transferases that use fatty acids from phospholipids as acyl donors. In this perspective, we review the current knowledge on these enzymes and propose that local modulation of phospholipids mediated by this pathway impacts the function and morphology of the targeted organelle. Triglycerides constitute an inert storage form for fatty acids deposited in lipid droplets and are mobilized to provide metabolic energy or membrane building blocks. The biosynthesis of triglycerides i...
Source: FEBS Letters - December 28, 2023 Category: Biochemistry Authors: Antonio D. Barbosa,
Symeon Siniossoglou Tags: Perspective Source Type: research
Front Cover
Cover illustration The cover refers to the article by Schwartz et al. ‘Structure–activity analysis suggests
an olfactory function for the unique antennal delta glutathione transferase of Apis mellifera’ in this issue. Image by Veronika Andrews from Pixabay (Source: FEBS Letters)
Source: FEBS Letters - December 27, 2023 Category: Biochemistry Tags: Issue Information Source Type: research
The journey toward clinical adoption of MALDI ‐MS based imaging proteomics: from current challenges to future expectations
In recent years, there is increasing evidence to suggest that matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) technology could offer support in clinical decision-making processes.Here, we discuss some of the recent technical developments associated underpinning this progress and outline some of the more promising clinical proteomics applications which have been developed with a clear goal towards implementation in mind. Among the spatial omics techniques available, mass spectrometry imaging (MSI) represents one of the most promising owing to its capability to map the distribution of hundre...
Source: FEBS Letters - December 23, 2023 Category: Biochemistry Authors: Isabella Piga,
Fulvio Magni,
Andrew Smith Tags: Review Source Type: research
A ‐kinase anchoring proteins are enriched in the central pair microtubules of motile cilia in Chlamydomonas reinhardtii
Effective motile cilia beating demands intricate coordination of various ciliary components, including radial spoke proteins, central pair proteins, and scaffold proteins. Among the scaffold proteins, A-kinase anchoring proteins (AKAPs) play a pivotal role. Thus far, the identity of central pair AKAPs has remained elusive. In our current study, we employed a blend of biochemical/biophysical methods to unveil three central pair AKAPs by utilizing an AKAP-interacting protein, FAP174, as a bait. Cilia are microtubule-based sensory organelles present in a number of eukaryotic cells. Mutations in the genes encoding ciliary prot...
Source: FEBS Letters - December 23, 2023 Category: Biochemistry Authors: Venkatramanan G. Rao,
Amruta A. Shendge,
Percival P. D ’Gama,
Elvis. A. F. Martis,
Shraddha Mehta,
Evans C. Coutinho,
Jacinta S. D’Souza Tags: Research Article Source Type: research
Redox and metabolic reprogramming in breast cancer and cancer ‐associated adipose tissue
This review focuses on deciphering the redox and metabolic profiles of breast cancer and associated adipose tissue as a part of a unique Warburg pseudo-organ. In the light of the coupled cancer and adipose tissue redox-metabolic reprogramming, mechanistic links to estrogen receptors, tumor microenvironment cell heterogeneity, inflammation, and circadian rhythms as important players affecting breast cancer development, progression and prognosis are also discussed (Created withBioRender.com). Redox and metabolic processes are tightly coupled in both physiological and pathological conditions. In cancer, their integration occu...
Source: FEBS Letters - December 23, 2023 Category: Biochemistry Authors: Tamara Zakic,
Vanja Pekovic ‐Vaughan,
Aleksandra Cvoro,
Aleksandra Korac,
Aleksandra Jankovic,
Bato Korac Tags: Review Source Type: research
Membranes that make fat: roles of membrane lipids as acyl donors for triglyceride synthesis and organelle function.
Certain eukaryotes can synthesise triacylglycerol using an acyl-CoA independent pathway. This activity is mediated by phospholipid diacylglycerol acyl transferases that use fatty acids from phospholipids as acyl donors. In this perspective, we review the current knowledge on these enzymes, and propose that local modulation of phospholipids mediated by this pathway impacts the function and morphology of the targeted organelle. Triglycerides constitute an inert storage form for fatty acids deposited in lipid droplets, and are mobilized to provide metabolic energy or membrane building blocks. The biosynthesis of triglycerides...
Source: FEBS Letters - December 23, 2023 Category: Biochemistry Authors: Antonio D. Barbosa,
Symeon Siniossoglou Tags: Perspective Source Type: research
Molecular mechanisms of perilipin protein function in lipid droplet metabolism
Lipid droplets are important for storage of energy and lipid components. The perilipin family in mammals includes five proteins (PLIN1 to PLIN5), all of which are abundant on the surface of lipid droplets in different tissues and play a role in lipid droplet stability and degradation by lipases. We discuss how perilipins interact with lipid droplets, their function and their implication in metabolic disease. Perilipins are abundant lipid droplet (LD) proteins present in all metazoans, and also in amoebozoa and fungi. Humans express five perilipins, which share a similar domain organization: an amino-terminal PAT domain and...
Source: FEBS Letters - December 23, 2023 Category: Biochemistry Authors: Elena Griseti,
Abdoul Akim Bello,
Eric Bieth,
Bayane Sabbagh,
Jason S. Iacovoni,
Jo ëlle Bigay,
Henrik Laurell,
Alenka Čopič Tags: Review Source Type: research
The mitotic checkpoint kinase BUB1 is a direct and actionable target of MYB in adenoid cystic carcinoma
In this study, we used immortalised breast epithelial cells and an inducibleMYB transgene as a model of ACC. Molecular profiling confirmed that MYB-driven gene expression causes a transition into an ACC-like state. Using this new cell model, we identified BUB1 as a targetable kinase directly controlled by MYB, whose pharmacological inhibition caused MYB-dependent synthetic lethality, growth arrest and apoptosis of patient-derived cells and organoids. (Source: FEBS Letters)
Source: FEBS Letters - December 20, 2023 Category: Biochemistry Authors: Ylenia Cicir ò,
Denise Ragusa,
Paloma Tejera Nevado,
Rossano Lattanzio,
Gianluca Sala,
Tessa DesRochers,
Melissa Millard,
Mattias K. Andersson,
Göran Stenman,
Arturo Sala Tags: Research Article Source Type: research
DAYWAKE implicates novel roles for circulating lipid ‐binding proteins as extracerebral regulators of daytime wake–sleep behavior
Drosophila daywake (dyw) is the first anti-siesta gene discovered. As in humans, key wake –sleep centers inDrosophila are found in the brain. Surprisingly, we show that the DYW protein, which belongs to the tubular lipid-binding protein (TULIP) superfamily, is not detected in the brain but accumulates in multiple organs and is secreted into the circulatory system, suggesting a novel signaling pathway for regulating wake –sleep balance. Sleep during the midday, commonly referred to as siesta, is a common trait of animals that mainly sleep during the night. Work usingDrosophila led to the identification of thedaywake (dy...
Source: FEBS Letters - December 19, 2023 Category: Biochemistry Authors: Gabriel Villegas,
Mathew T. Pereira,
Cameron R. Love,
Isaac Edery Tags: Research Letter Source Type: research
