Strigolactone biosynthesis in rice can occur via a 9 ‐cis‐3‐OH‐10′‐apo‐β‐carotenal intermediate
In this study, we supplied the13C-labeled 9-cis-3-OH- β-apo-10′-carotenal to rice seedlings and monitored the synthesis of SLs using liquid chromatography-mass spectrometry (LC–MS) andStriga bioassay. We further validated the biological activity of 9-cis-3-OH- β-apo-10′-carotenal-derived SLs using theccd7/d17 SL-deficient mutant, which demonstrated increasedStriga seed-germinating activity and partial rescue of tiller numbers and plant height. Our results establish 9-cis-3-OH- β-apo-10′-carotenal as a significant SL biosynthetic intermediate with implications for understanding plant hormonal functions and potent...
Source: FEBS Letters - February 20, 2024 Category: Biochemistry Authors: Jian You Wang,
Guan ‐Ting Erica Chen,
Aparna Balakrishna,
Muhammad Jamil,
Lamis Berqdar,
Salim Al‐Babili Tags: Research Letter Source Type: research
The positive allosteric modulator BPAM344 and L ‐glutamate introduce an active‐like structure of the ligand‐binding domain of GluK2
This study may aid in the design of compounds targeting kainate receptors, expanding their potential as targets for the treatment of brain diseases. (Source: FEBS Letters)
Source: FEBS Letters - February 19, 2024 Category: Biochemistry Authors: Yasmin Bay,
Mie Egeberg Jeppesen,
Karla Frydenvang,
Pierre Francotte,
Bernard Pirotte,
Darryl S. Pickering,
Anders Skov Kristensen,
Jette Sandholm Kastrup Tags: Research Letter Source Type: research
Identification and characterization of nuclear localization signals in the circumsporozoite protein of Plasmodium falciparum
In this study, we have identified and characterized two NLSs in the circumsporozoite protein ofPlasmodium falciparum (Pf-CSP). Both NLSs in thePf-CSP contain clusters of lysine and arginine residues essential for specific interactions with the conserved tryptophan and asparagine residues of importin- α, facilitating nuclear translocation ofPf-CSP. While the two NLSs ofPf-CSP function independently and are both crucial for nuclear localization, a single NLS ofPf-CSP leads to weak nuclear localization. These findings shed light on the mechanism of nuclear penetrability of secretory proteins ofPlasmodium proteins. (Source: FEBS Letters)
Source: FEBS Letters - February 19, 2024 Category: Biochemistry Authors: Akshaykumar Nanaji Shrikondawar,
Kiranmai Chennoju,
Debasish Kumar Ghosh,
Akash Ranjan Tags: Research Letter Source Type: research
Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress
What happens if an organism can no longer react to cytosolic proteotoxic stress? To answer this question, we deleted or depleted the stress-responsive transcription factors Msn2, Msn4 and Hsf1 inS. cerevisiae. We show that Msn2/4 broadly reprogram transcription while Hsf1 induces mainly the synthesis of molecular chaperones and reverses the transcriptional response upon prolonged mild heat stress (adaptation). The response to proteotoxic stresses such as heat shock allows organisms to maintain protein homeostasis under changing environmental conditions. We asked what happens if an organism can no longer react to cytosol...
Source: FEBS Letters - February 17, 2024 Category: Biochemistry Authors: Moritz M ühlhofer,
Felix Offensperger,
Sarah Reschke,
Georg Wallmann,
Gergely Csaba,
Evi Berchtold,
Maximilian Riedl,
Helmut Blum,
Martin Haslbeck,
Ralf Zimmer,
Johannes Buchner Tags: Research Article Source Type: research
CIDE proteins and their regulatory mechanisms in lipid droplet fusion and growth
Three members of CIDE proteins play important roles in several aspects of lipid metabolism through different tissue distribution, cellular localization, and interacting proteins. We review the molecular and cellular mechanisms of CIDE proteins in controlling lipid droplet fusion and lipid storage, promoting lipid secretion, and regulating activities of transcriptional factors. The cell death-inducing DFF45-like effector (CIDE) proteins, including Cidea, Cideb, and Cidec/Fsp27, regulate various aspects of lipid homeostasis, including lipid storage, lipolysis, and lipid secretion. This review focuses on the physiological rol...
Source: FEBS Letters - February 15, 2024 Category: Biochemistry Authors: Li Xu,
Lizhen Li,
Lingzhi Wu,
Peng Li,
Feng ‐Jung Chen Tags: Review Source Type: research
Cell competition: emerging signaling and unsolved questions
Cell competition is a form of cell –cell interaction whereby cells with higher fitness (“winners”) actively eliminate neighboring less-fit cells (“losers”) in the growing tissue. In this review, we summarize and discuss emerging signaling mechanisms and critical unsolved questions in cell competition. Multicellular communities have an intrinsic mechanism that optimizes their structure and functionvia cell –cell communication. One of the driving forces for such self-organization of the multicellular system is cell competition, the elimination of viable unfit or deleterious cellsvia cell –cell interaction. Stud...
Source: FEBS Letters - February 14, 2024 Category: Biochemistry Authors: Rina Nagata,
Tatsushi Igaki Tags: Review Source Type: research
Correction to “Gly56 in the synthetic site of isoleucyl‐tRNA synthetase confers specificity and maintains communication with the editing site”
(Source: FEBS Letters)
Source: FEBS Letters - February 14, 2024 Category: Biochemistry Tags: Correction Source Type: research
The three ‐dimensional structure of the Vint domain from Tetrahymena thermophila suggests a ligand‐regulated cleavage mechanism by the HINT fold
This study unveiled the HINT structure, including an adduct recognition region, explored interactions with heparin, and suggested a regulatory role in auto-processing. These findings enhance our understanding of HINT fold evolution and its potential biotechnological uses. Vint proteins have been identified in unicellular metazoans as a novel hedgehog-related gene family, merging the von Willebrand factor type A domain and the Hedgehog/INTein (HINT) domains. We present the first three-dimensional structure of the Vint domain fromTetrahymena thermophila corresponding to the auto-processing domain of hedgehog proteins, sheddi...
Source: FEBS Letters - February 14, 2024 Category: Biochemistry Authors: Hideo Iwa ï,
Hannes M. Beyer,
Julia E. M. Johansson,
Mi Li,
Alexander Wlodawer Tags: Research Letter Source Type: research
