A potassium-chloride co-transporter with altered genome architecture functions as a suppressor in glioma
J Cell Mol Med. 2024 May;28(9):e18352. doi: 10.1111/jcmm.18352.ABSTRACTGliomas, the most lethal tumours in brain, have a poor prognosis despite accepting standard treatment. Limited benefits from current therapies can be attributed to genetic, epigenetic and microenvironmental cues that affect cell programming and drive tumour heterogeneity. Through the analysis of Hi-C data, we identified a potassium-chloride co-transporter SLC12A5 associated with disrupted topologically associating domain which was downregulated in tumour tissues. Multiple independent glioma cohorts were included to analyse the characterization of SLC12A...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Hongwei Liu Zhouyang Pan Xuelei Lin Long Chen Qi Yang Wei Zhang Luohuan Dai Yihao Zhang Wang Li Yinhua Chen Kang Peng Siyi Wanggou Feiyue Zeng Xuejun Li Source Type: research
MFNet: Meta-learning based on frequency-space mix for MRI segmentation in nasopharyngeal carcinoma
J Cell Mol Med. 2024 May;28(9):e18355. doi: 10.1111/jcmm.18355.ABSTRACTDeep learning techniques have been applied to medical image segmentation and demonstrated expert-level performance. Due to the poor generalization abilities of the models in the deployment in different centres, common solutions, such as transfer learning and domain adaptation techniques, have been proposed to mitigate this issue. However, these solutions necessitate retraining the models with target domain data and annotations, which limits their deployment in clinical settings in unseen domains. We evaluated the performance of domain generalization met...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Yin Li Qi Chen Hao Li Song Wang Nutan Chen Ting Han Kai Wang Qingqing Yu Zhantao Cao Jun Tang Source Type: research
Comparative evaluation of bone marrow and dental pulp mesenchymal stem cells for motor functional recovery in rat sciatic nerve injury
This study delves into the impact of mesenchymal stem cells derived from bone marrow (BM-MSCs) and those sourced from dental pulp (DP-MSCs) on the recovery of motor function and morphological aspects of the rat's sciatic nerve after crush injuries. The findings highlight that the groups treated with BM-MSCs, DP-MSCs or a combination of both (BM + DP-MSCs) displayed enhanced sciatic functional index values when juxtaposed with the sham group. This points to bettered motor functionalities. A deeper morphological analysis showed that all the groups had retained perineurium structure and fascicular arrangement. Notably, the sh...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Elgin Or çum Uzunlu Zeki O ğurtan Source Type: research
Decoding dynamic interactions between EGFR-TKD and DAC through computational and experimental approaches: A novel breakthrough in lung melanoma treatment
This study focuses on CTX-1, exploring its interaction with the pivotal EGFR-TKD protein, a crucial target in lung cancer therapeutics. A meticulous molecular docking analysis revealed that CTX-1 exhibits a noteworthy binding affinity of -7.9 kcal/mol, challenging Erlotinib, a conventional lung cancer medication, which displayed a binding affinity of -7.3 kcal/mol. For a deeper understanding of CTX-1's molecular mechanics, this study employed rigorous 100-ns molecular dynamics simulations, demonstrating CTX-1's remarkable stability in comparison with erlotinib. The Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBS...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Rajesh Kumar Meher Showkat Ahmad Mir Kritika Singh Nobendu Mukerjee Binata Nayak Ajoy Kumer Torki A Zughaibi Mohd Shahnawaz Khan Shams Tabrez Source Type: research
Orexin-A mediates glioblastoma proliferation inhibition by increasing ferroptosis triggered by unstable iron pools and GPX4 depletion
In this report, we provide the first evidence that orexin-A exerts inhibitory effects on GBM proliferation via the induction of ferroptosis. This induction is achieved by instigating an unsustainable increase in iron levels and depletion of GPX4. Moreover, the regulation of TFRC, FTH1 and GPX4 expression through the targeting of NFE2L2 appears to be one of the potential mechanisms underlying orexin-A-induced ferroptosis.PMID:38685674 | DOI:10.1111/jcmm.18318 (Source: Molecular Medicine)
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Rengzheng Huan Jiqin Zhang Jianhe Yue Sha Yang Guoqiang Han Yuan Cheng Ying Tan Source Type: research
Long non-coding RNA H19 mediates osteogenic differentiation of bone marrow mesenchymal stem cells through the miR-29b-3p/DKK1 axis
This study aimed to explore the molecular mechanism of long non-coding RNA H19 in osteoporosis after SCI and provide new research directions for existing prevention strategies. We used small interfering RNA to knockdown H19 expression and regulated miR-29b-2p expression using miR-29b-3p mimetics and inhibitors. Western blotting, real-time fluorescence quantitative PCR, Alizarin red staining, alkaline phosphatase staining and double-luciferase reporter gene assays were used to assess gene expression, osteogenic ability and binding sites. lncRNA H19 was upregulated in BMSCs from the osteoporosis group, whereas miR-29b-3p was...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Sen Qin Da Liu Source Type: research
The role of innate lymphoid cells in systemic lupus erythematosus
Cytokine. 2024 Apr 28;179:156623. doi: 10.1016/j.cyto.2024.156623. Online ahead of print.ABSTRACTSystemic lupus erythematosus (SLE) is a connective tissue disorder that affects various body systems. Both the innate and adaptive immunity contribute to the onset and progression of SLE. The main mechanism of SLE is an excessive immune response of immune cells to autoantigens, which leads to systemic inflammation and inflammation-induced organ damage. Notably, a subset of innate immune cells known as innate lymphoid cells (ILCs) has recently emerged. ILCs are pivotal in the early stages of infection; participate in immune resp...
Source: Cytokine - April 30, 2024 Category: Molecular Biology Authors: Yong Chen Xiaotian Gu Kunyu Cao Miao Tu Wan Liu Jiyu Ju Source Type: research
Screening of the effective sites of Cichorium glandulosum against hyperuricemia combined with hyperlipidemia and its network pharmacology analysis
This study aims to explore the pharmacological effects and mechanisms of Cichorium glandulosum on this comorbidity through a combination of animal experiments, network pharmacology, and molecular docking techniques. A rat model of hyperuricaemia combined with hyperlipidaemia was established through a high-fat and high-purine diet, and the effective parts of the aqueous extract of Cichorium glandulosum to reduce uric acid and lipid levels were screened and the components of the parts were analysed by LC-MS/MS. The active components, core targets, and key pathways were analysed using network pharmacology and validated by mol...
Source: Computational Biology and Chemistry - April 30, 2024 Category: Bioinformatics Authors: Zhiguo Yang Bo Lyu Bin Ma Yuefeng Gao Dongmei Qin Source Type: research
Harnessing the 14-3-3 protein-protein interaction network
Curr Opin Struct Biol. 2024 Apr 28;86:102822. doi: 10.1016/j.sbi.2024.102822. Online ahead of print.ABSTRACTProtein-protein interactions (PPIs) play a critical role in cellular signaling and represent interesting targets for therapeutic intervention. 14-3-3 proteins integrate many signaling targets via PPIs and are frequently implicated in disease, making them intriguing drug targets. Here, we review the recent advances in the 14-3-3 field. It will discuss the roles 14-3-3 proteins play within the cell, elucidation of their expansive interactome, and the complex mechanisms that underpin their function. In addition, the rev...
Source: Current Opinion in Structural Biology - April 30, 2024 Category: Biology Authors: Paulo Pitasse-Santos Isaac Hewitt-Richards Malsha D Abeywickrama Wijewardana Sooriyaarachchi Richard G Doveston Source Type: research
Expression of human dCTP pyrophosphatase 1 (DCTPP1) and its association with cisplatin resistance characteristics in ovarian cancer
In this study, we aimed to understand the role of DCTPP1 in cancer progression and cisplatin response. Using publicly available databases, we analysed the expression and clinical significance of DCTPP1 in ovarian cancer. Our bioinformatics analysis confirmed that DCTPP1 is significantly overexpressed in ovarian cancer and is closely associated with tumour progression and poor prognosis after cisplatin treatment. We also found that DCTPP1 located in oxidoreductase complex and may be involved in various biological processes related to cisplatin resistance, including pyrimidine nucleotide metabolism, the P53 signalling pathwa...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Yu Wang Xiangyun Chen Qiduan Chen Tiancai Liu Yingsong Wu Liping Huang Yao Chen Source Type: research
Single cell sequencing data identify distinct B cell and fibroblast populations in stricturing Crohn's disease
J Cell Mol Med. 2024 May;28(9):e18344. doi: 10.1111/jcmm.18344.ABSTRACTSingle cell RNA sequencing of human full thickness Crohn's disease (CD) small bowel resection specimens was used to identify potential therapeutic targets for stricturing (S) CD. Using an unbiased approach, 16 cell lineages were assigned within 14,539 sequenced cells from patient-matched SCD and non-stricturing (NSCD) preparations. SCD and NSCD contained identical cell types. Amongst immune cells, B cells and plasma cells were selectively increased in SCD samples. B cell subsets suggested formation of tertiary lymphoid tissue in SCD and compared with NS...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: David T Humphreys Amy Lewis Belen Pan-Castillo Giulio Berti Charles Mein Eva Wozniak Hannah Gordon Radha Gadhok Annamaria Minicozzi Joanna ChinAleong Roger Feakins Eleni Giannoulatou Louisa K James Andrew J Stagg James Oliver Lindsay Andrew Silver Source Type: research
Multiomics Analysis of Disulfidptosis Patterns and Integrated Machine Learning to Predict Immunotherapy Response in Lung Adenocarcinoma
CONCLUSION: Our research introduces an innovative prognostic risk model predicated upon disulfidptosis genes for patients afflicted with Lung Adenocarcinoma (LUAD). This model proficiently forecasts the survival rates and therapeutic outcomes for LUAD patients, thereby delineating the high-risk population with distinctive immune cell infiltration and a state of immunosuppression. Furthermore, NAPSA can inhibit the proliferation and invasion capabilities of LUAD cells, thereby identifying new molecules for clinical targeted therapy.PMID:38685772 | DOI:10.2174/0109298673313281240425050032 (Source: Current Medicinal Chemistry)
Source: Current Medicinal Chemistry - April 30, 2024 Category: Chemistry Authors: Junzhi Liu Huimin Li Nannan Zhang Qiuping Dong Zheng Liang Source Type: research
Polymeric Molecular Envelope Technology for Improved Therapeutics Efficacy: A Review
Curr Med Chem. 2024 Apr 29. doi: 10.2174/0109298673290370240425092350. Online ahead of print.ABSTRACTThe review explores the enhancement of therapeutic efficacy through the innovative use of polymeric molecular envelope technology (MET). It delves into the diverse methods employed to achieve superior therapeutic outcomes, shedding light on strategies for improving drug delivery and bioavailability. MET is a promising approach to improve the solubility and bioavailability of poorly water-soluble drugs. This technology involves the use of a molecular envelope of cyclic oligosaccharides called cyclodextrins, which is a supram...
Source: Current Medicinal Chemistry - April 30, 2024 Category: Chemistry Authors: Jigar Vyas Nensi Raytthatha Bhupendra G Prajapati Sudarshan Singh Source Type: research
Developing theragnostics for Alzheimer's disease: Insights from cancer treatment
Int J Biol Macromol. 2024 Apr 27:131925. doi: 10.1016/j.ijbiomac.2024.131925. Online ahead of print.ABSTRACTThe prevalence of Alzheimer's disease (AD) and its associated economic and societal burdens are on the rise, but there are no curative treatments for AD. Interestingly, this neurodegenerative disease shares several biological and pathophysiological features with cancer, including cell-cycle dysregulation, angiogenesis, mitochondrial dysfunction, protein misfolding, and DNA damage. However, the genetic factors contributing to the overlap in biological processes between cancer and AD have not been actively studied. In ...
Source: International Journal of Biological Macromolecules - April 30, 2024 Category: Biochemistry Authors: Hyun-Ju Lee Hee-Jeong Choi Yoo Joo Jeong Yoon-Hee Na Jin Tae Hong Ji Min Han Hyang-Sook Hoe Key-Hwan Lim Source Type: research
Composition-antifreeze property relationships of gelatin and the corresponding mechanisms
This study provided some references to further enhance antifreeze performance of gelatin by modulating fragment composition.PMID:38685545 | DOI:10.1016/j.ijbiomac.2024.131941 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 30, 2024 Category: Biochemistry Authors: Shichen Zhu Yan Jin Jiehang Yu Wenting Yang Hua Song Zhengpeng Wei Shulai Liu Yuting Ding Xuxia Zhou Source Type: research
