Orexin-A mediates glioblastoma proliferation inhibition by increasing ferroptosis triggered by unstable iron pools and GPX4 depletion
In this report, we provide the first evidence that orexin-A exerts inhibitory effects on GBM proliferation via the induction of ferroptosis. This induction is achieved by instigating an unsustainable increase in iron levels and depletion of GPX4. Moreover, the regulation of TFRC, FTH1 and GPX4 expression through the targeting of NFE2L2 appears to be one of the potential mechanisms underlying orexin-A-induced ferroptosis.PMID:38685674 | DOI:10.1111/jcmm.18318 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Rengzheng Huan Jiqin Zhang Jianhe Yue Sha Yang Guoqiang Han Yuan Cheng Ying Tan Source Type: research
Fangchinoline inhibits growth and biofilm of Candida albicans by inducing ROS overproduction
J Cell Mol Med. 2024 May;28(9):e18354. doi: 10.1111/jcmm.18354.ABSTRACTInfections caused by Candida species, especially Candida albicans, threaten the public health and create economic burden. Shortage of antifungals and emergence of drug resistance call for new antifungal therapies while natural products were attractive sources for developing new drugs. In our study, fangchinoline, a bis-benzylisoquinoline alkaloid from Chinese herb Stephania tetrandra S. Moore, exerted antifungal effects on planktonic growth of several Candida species including C. albicans, with MIC no more than 50 μg/mL. In addition, results from micro...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Longfei Yang Xiaonan Wang Zhiming Ma Yujie Sui Xin Liu Source Type: research
Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis
In conclusion, GPX4 and ferroptosis regulated the progression of TMJOA. Targeting ferroptosis might be an effective therapeutic strategy for TMJOA patients in the clinic.PMID:38686488 | DOI:10.1111/jcmm.18377 (Source: Molecular Medicine)
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Chunyan Pang Hongmei Zhang Yi Liu Na Tang Kun Tian Yandong Mu Xue Li Li Xiao Source Type: research
Sirt7/HIC1 complex participates in hyperglycaemia-mediated EndMT via modulation of SDC1 expression in diabetic kidney disease and metabolic memory
This study demonstrated that the hyperglycaemia-mediated interaction between Sirt7 and HIC1 exerts a role in the metabolic memory in DKD by inactivating SDC1 transcription and mediating EndMT despite glucose normalization in HGECs.PMID:38686489 | DOI:10.1111/jcmm.18336 (Source: Molecular Medicine)
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Lihong Lu Minmin Zhu Qichao Wu Zhirong Sun Xiangyuan Chen Changhong Miao Source Type: research
Malvidin attenuates trauma-induced heterotopic ossification of tendon in rats by targeting Rheb for degradation via the ubiquitin-proteasome pathway
This study aimed to investigate the effect of MA in treating trauma-induced HO and unravel its underlying mechanisms. Herein, the effectiveness of MA in preventing HO formation was assessed through local injection in a rat model. The potential mechanism underlying MA's treatment was investigated in the tendon-resident progenitor cells of tendon-derived stem cells (TDSCs), exploring its pathway in HO formation. The findings demonstrated that MA effectively hindered the osteogenic differentiation of TDSCs by inhibiting the mTORC1 signalling pathway, consequently impeding the progression of trauma-induced HO of Achilles tendo...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Huaji Jiang Yan Ding Xuemei Lin Qinyu Tian Yakui Liu Hebei He Yongfu Wu Xinggui Tian Stefan Zwingenberger Source Type: research
The role of anoctamin 1 in liver disease
J Cell Mol Med. 2024 May;28(9):e18320. doi: 10.1111/jcmm.18320.ABSTRACTLiver diseases include all types of viral hepatitis, alcoholic liver disease (ALD), nonalcoholic fatty liver disease (NAFLD), cirrhosis, liver failure (LF) and hepatocellular carcinoma (HCC). Liver disease is now one of the leading causes of disease and death worldwide, which compels us to better understand the mechanisms involved in the development of liver diseases. Anoctamin 1 (ANO1), a calcium-activated chloride channel (CaCC), plays an important role in epithelial cell secretion, proliferation and migration. ANO1 plays a key role in transcriptional...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Xin Li Yongfeng Wang Li Zhang Shun Yao Qian Liu Hai Jin Biguang Tuo Source Type: research
A potassium-chloride co-transporter with altered genome architecture functions as a suppressor in glioma
J Cell Mol Med. 2024 May;28(9):e18352. doi: 10.1111/jcmm.18352.ABSTRACTGliomas, the most lethal tumours in brain, have a poor prognosis despite accepting standard treatment. Limited benefits from current therapies can be attributed to genetic, epigenetic and microenvironmental cues that affect cell programming and drive tumour heterogeneity. Through the analysis of Hi-C data, we identified a potassium-chloride co-transporter SLC12A5 associated with disrupted topologically associating domain which was downregulated in tumour tissues. Multiple independent glioma cohorts were included to analyse the characterization of SLC12A...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Hongwei Liu Zhouyang Pan Xuelei Lin Long Chen Qi Yang Wei Zhang Luohuan Dai Yihao Zhang Wang Li Yinhua Chen Kang Peng Siyi Wanggou Feiyue Zeng Xuejun Li Source Type: research
MFNet: Meta-learning based on frequency-space mix for MRI segmentation in nasopharyngeal carcinoma
J Cell Mol Med. 2024 May;28(9):e18355. doi: 10.1111/jcmm.18355.ABSTRACTDeep learning techniques have been applied to medical image segmentation and demonstrated expert-level performance. Due to the poor generalization abilities of the models in the deployment in different centres, common solutions, such as transfer learning and domain adaptation techniques, have been proposed to mitigate this issue. However, these solutions necessitate retraining the models with target domain data and annotations, which limits their deployment in clinical settings in unseen domains. We evaluated the performance of domain generalization met...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Yin Li Qi Chen Hao Li Song Wang Nutan Chen Ting Han Kai Wang Qingqing Yu Zhantao Cao Jun Tang Source Type: research
Comparative evaluation of bone marrow and dental pulp mesenchymal stem cells for motor functional recovery in rat sciatic nerve injury
This study delves into the impact of mesenchymal stem cells derived from bone marrow (BM-MSCs) and those sourced from dental pulp (DP-MSCs) on the recovery of motor function and morphological aspects of the rat's sciatic nerve after crush injuries. The findings highlight that the groups treated with BM-MSCs, DP-MSCs or a combination of both (BM + DP-MSCs) displayed enhanced sciatic functional index values when juxtaposed with the sham group. This points to bettered motor functionalities. A deeper morphological analysis showed that all the groups had retained perineurium structure and fascicular arrangement. Notably, the sh...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Elgin Or çum Uzunlu Zeki O ğurtan Source Type: research
Decoding dynamic interactions between EGFR-TKD and DAC through computational and experimental approaches: A novel breakthrough in lung melanoma treatment
This study focuses on CTX-1, exploring its interaction with the pivotal EGFR-TKD protein, a crucial target in lung cancer therapeutics. A meticulous molecular docking analysis revealed that CTX-1 exhibits a noteworthy binding affinity of -7.9 kcal/mol, challenging Erlotinib, a conventional lung cancer medication, which displayed a binding affinity of -7.3 kcal/mol. For a deeper understanding of CTX-1's molecular mechanics, this study employed rigorous 100-ns molecular dynamics simulations, demonstrating CTX-1's remarkable stability in comparison with erlotinib. The Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBS...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Rajesh Kumar Meher Showkat Ahmad Mir Kritika Singh Nobendu Mukerjee Binata Nayak Ajoy Kumer Torki A Zughaibi Mohd Shahnawaz Khan Shams Tabrez Source Type: research
Orexin-A mediates glioblastoma proliferation inhibition by increasing ferroptosis triggered by unstable iron pools and GPX4 depletion
In this report, we provide the first evidence that orexin-A exerts inhibitory effects on GBM proliferation via the induction of ferroptosis. This induction is achieved by instigating an unsustainable increase in iron levels and depletion of GPX4. Moreover, the regulation of TFRC, FTH1 and GPX4 expression through the targeting of NFE2L2 appears to be one of the potential mechanisms underlying orexin-A-induced ferroptosis.PMID:38685674 | DOI:10.1111/jcmm.18318 (Source: Molecular Medicine)
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Rengzheng Huan Jiqin Zhang Jianhe Yue Sha Yang Guoqiang Han Yuan Cheng Ying Tan Source Type: research
Long non-coding RNA H19 mediates osteogenic differentiation of bone marrow mesenchymal stem cells through the miR-29b-3p/DKK1 axis
This study aimed to explore the molecular mechanism of long non-coding RNA H19 in osteoporosis after SCI and provide new research directions for existing prevention strategies. We used small interfering RNA to knockdown H19 expression and regulated miR-29b-2p expression using miR-29b-3p mimetics and inhibitors. Western blotting, real-time fluorescence quantitative PCR, Alizarin red staining, alkaline phosphatase staining and double-luciferase reporter gene assays were used to assess gene expression, osteogenic ability and binding sites. lncRNA H19 was upregulated in BMSCs from the osteoporosis group, whereas miR-29b-3p was...
Source: Molecular Medicine - April 30, 2024 Category: Molecular Biology Authors: Sen Qin Da Liu Source Type: research
The role of innate lymphoid cells in systemic lupus erythematosus
Cytokine. 2024 Apr 28;179:156623. doi: 10.1016/j.cyto.2024.156623. Online ahead of print.ABSTRACTSystemic lupus erythematosus (SLE) is a connective tissue disorder that affects various body systems. Both the innate and adaptive immunity contribute to the onset and progression of SLE. The main mechanism of SLE is an excessive immune response of immune cells to autoantigens, which leads to systemic inflammation and inflammation-induced organ damage. Notably, a subset of innate immune cells known as innate lymphoid cells (ILCs) has recently emerged. ILCs are pivotal in the early stages of infection; participate in immune resp...
Source: Cytokine - April 30, 2024 Category: Molecular Biology Authors: Yong Chen Xiaotian Gu Kunyu Cao Miao Tu Wan Liu Jiyu Ju Source Type: research
Screening of the effective sites of Cichorium glandulosum against hyperuricemia combined with hyperlipidemia and its network pharmacology analysis
This study aims to explore the pharmacological effects and mechanisms of Cichorium glandulosum on this comorbidity through a combination of animal experiments, network pharmacology, and molecular docking techniques. A rat model of hyperuricaemia combined with hyperlipidaemia was established through a high-fat and high-purine diet, and the effective parts of the aqueous extract of Cichorium glandulosum to reduce uric acid and lipid levels were screened and the components of the parts were analysed by LC-MS/MS. The active components, core targets, and key pathways were analysed using network pharmacology and validated by mol...
Source: Computational Biology and Chemistry - April 30, 2024 Category: Bioinformatics Authors: Zhiguo Yang Bo Lyu Bin Ma Yuefeng Gao Dongmei Qin Source Type: research
Harnessing the 14-3-3 protein-protein interaction network
Curr Opin Struct Biol. 2024 Apr 28;86:102822. doi: 10.1016/j.sbi.2024.102822. Online ahead of print.ABSTRACTProtein-protein interactions (PPIs) play a critical role in cellular signaling and represent interesting targets for therapeutic intervention. 14-3-3 proteins integrate many signaling targets via PPIs and are frequently implicated in disease, making them intriguing drug targets. Here, we review the recent advances in the 14-3-3 field. It will discuss the roles 14-3-3 proteins play within the cell, elucidation of their expansive interactome, and the complex mechanisms that underpin their function. In addition, the rev...
Source: Current Opinion in Structural Biology - April 30, 2024 Category: Biology Authors: Paulo Pitasse-Santos Isaac Hewitt-Richards Malsha D Abeywickrama Wijewardana Sooriyaarachchi Richard G Doveston Source Type: research
