Comparative evaluation of hesperetin-loaded graphene oxide nanosheets (Hsp-GO) as a drug delivery system for colon cancer: synthesis and anticancer efficiency assessment
CONCLUSIONS: These study highlight the potential of Hsp-GO as a promising anti-cancer nano-drug for colon cancer therapy.PMID:38683228 | DOI:10.1007/s11033-024-09504-7 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 29, 2024 Category: Molecular Biology Authors: Arezou Sabbagh Hadi Mehdi Haghi Abolfazl Barzegar Mohammad Ali Hosseinpour Feizi Source Type: research
Glutamine-mediated epigenetic regulation of cFLIP underlies resistance to TRAIL in pancreatic cancer
In this study, we demonstrated that glutamine (Gln) endows PDAC cells with resistance to TRAIL through KDM4C-mediated epigenetic regulation of cFLIP. Inhibition of glutaminolysis significantly reduced the cFLIP level, leading to TRAIL-mediated formation of death-inducing signaling complexes. Overexpression of cFLIP dramatically rescued PDAC cells from TRAIL/Gln deprivation-induced apoptosis. Alpha-Ketoglutarate (aKG) supplementation significantly reversed the decrease in the cFLIP level induced by glutaminolysis inhibition and rescued PDAC cells from TRAIL/Gln deprivation-induced apoptosis. Knockdown of glutamic-oxaloaceti...
Source: Molecular Medicine - April 29, 2024 Category: Molecular Biology Authors: Ji Hye Kim Jinyoung Lee Se Seul Im Boyun Kim Eun-Young Kim Hyo-Jin Min Jinbeom Heo Eun-Ju Chang Kyung-Chul Choi Dong-Myung Shin Jaekyoung Son Source Type: research
CBX3 antagonizes IFN γ/STAT1/PD-L1 axis to modulate colon inflammation and CRC chemosensitivity
EMBO Mol Med. 2024 Apr 29. doi: 10.1038/s44321-024-00066-6. Online ahead of print.ABSTRACTAs an important immune stimulator and modulator, IFNγ is crucial for gut homeostasis and its dysregulation links to diverse colon pathologies, such as colitis and colorectal cancer (CRC). Here, we demonstrated that the epigenetic regulator, CBX3 (also known as HP1γ) antagonizes IFNγ signaling in the colon epithelium by transcriptionally repressing two critical IFNγ-responsive genes: STAT1 and CD274 (encoding Programmed death-ligand 1, PD-L1). Accordingly, CBX3 deletion resulted in chronic mouse colon inflammation, accompanied by u...
Source: Molecular Medicine - April 29, 2024 Category: Molecular Biology Authors: Yao Xiang Jorge Mata-Garrido Yuanji Fu Christophe Desterke Eric Batsch é Ahmed Hama ï Christine Sedlik Youssouf Sereme David Skurnik Abdelali Jalil Rachel Onifarasoaniaina Eric Frapy Jean-Christophe Beche Razack Alao Eliane Piaggio Laurence Arbibe Yunhu Source Type: research
Inhibition of asparagine synthetase effectively retards polycystic kidney disease progression
EMBO Mol Med. 2024 Apr 29. doi: 10.1038/s44321-024-00071-9. Online ahead of print.ABSTRACTPolycystic kidney disease (PKD) is a genetic disorder characterized by bilateral cyst formation. We showed that PKD cells and kidneys display metabolic alterations, including the Warburg effect and glutaminolysis, sustained in vitro by the enzyme asparagine synthetase (ASNS). Here, we used antisense oligonucleotides (ASO) against Asns in orthologous and slowly progressive PKD murine models and show that treatment leads to a drastic reduction of total kidney volume (measured by MRI) and a prominent rescue of renal function in the mouse...
Source: Molecular Medicine - April 29, 2024 Category: Molecular Biology Authors: Sara Clerici Christine Podrini Davide Stefanoni Gianfranco Distefano Laura Cassina Maria Elena Steidl Laura Tronci Tamara Canu Marco Chiaravalli Daniel Spies Thomas A Bell Ana Sh Costa Antonio Esposito Angelo D'Alessandro Christian Frezza Angela Bachi Ale Source Type: research
GP64-pseudotyped lentiviral vectors target liver endothelial cells and correct hemophilia A mice
We report that VSV.G-LV transduced hepatocytes better than GP64-LV, however the latter showed improved transduction of liver sinusoidal endothelial cells (LSEC). Combining GP64-pseudotyping with the high surface content of the phagocytosis inhibitor CD47 further enhanced LSEC transduction. Coagulation factor VIII (FVIII), the gene mutated in hemophilia A, is naturally expressed by LSEC, thus we exploited GP64-LV to deliver a FVIII transgene under the control of the endogenous FVIII promoter and achieved therapeutic amounts of FVIII and correction of hemophilia A mice.PMID:38684862 | DOI:10.1038/s44321-024-00072-8 (Source: Molecular Medicine)
Source: Molecular Medicine - April 29, 2024 Category: Molecular Biology Authors: Michela Milani Cesare Canepari Simone Assanelli Simone Merlin Ester Borroni Francesco Starinieri Mauro Biffi Fabio Russo Anna Fabiano Desir èe Zambroni Andrea Annoni Luigi Naldini Antonia Follenzi Alessio Cantore Source Type: research
