The role of anoctamin 1 in liver disease
J Cell Mol Med. 2024 May;28(9):e18320. doi: 10.1111/jcmm.18320.ABSTRACTLiver diseases include all types of viral hepatitis, alcoholic liver disease (ALD), nonalcoholic fatty liver disease (NAFLD), cirrhosis, liver failure (LF) and hepatocellular carcinoma (HCC). Liver disease is now one of the leading causes of disease and death worldwide, which compels us to better understand the mechanisms involved in the development of liver diseases. Anoctamin 1 (ANO1), a calcium-activated chloride channel (CaCC), plays an important role in epithelial cell secretion, proliferation and migration. ANO1 plays a key role in transcriptional...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Xin Li Yongfeng Wang Li Zhang Shun Yao Qian Liu Hai Jin Biguang Tuo Source Type: research
MFNet: Meta-learning based on frequency-space mix for MRI segmentation in nasopharyngeal carcinoma
J Cell Mol Med. 2024 May;28(9):e18355. doi: 10.1111/jcmm.18355.ABSTRACTDeep learning techniques have been applied to medical image segmentation and demonstrated expert-level performance. Due to the poor generalization abilities of the models in the deployment in different centres, common solutions, such as transfer learning and domain adaptation techniques, have been proposed to mitigate this issue. However, these solutions necessitate retraining the models with target domain data and annotations, which limits their deployment in clinical settings in unseen domains. We evaluated the performance of domain generalization met...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Yin Li Qi Chen Hao Li Song Wang Nutan Chen Ting Han Kai Wang Qingqing Yu Zhantao Cao Jun Tang Source Type: research
Comparative evaluation of bone marrow and dental pulp mesenchymal stem cells for motor functional recovery in rat sciatic nerve injury
This study delves into the impact of mesenchymal stem cells derived from bone marrow (BM-MSCs) and those sourced from dental pulp (DP-MSCs) on the recovery of motor function and morphological aspects of the rat's sciatic nerve after crush injuries. The findings highlight that the groups treated with BM-MSCs, DP-MSCs or a combination of both (BM + DP-MSCs) displayed enhanced sciatic functional index values when juxtaposed with the sham group. This points to bettered motor functionalities. A deeper morphological analysis showed that all the groups had retained perineurium structure and fascicular arrangement. Notably, the sh...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Elgin Or çum Uzunlu Zeki O ğurtan Source Type: research
Single cell sequencing data identify distinct B cell and fibroblast populations in stricturing Crohn's disease
J Cell Mol Med. 2024 May;28(9):e18344. doi: 10.1111/jcmm.18344.ABSTRACTSingle cell RNA sequencing of human full thickness Crohn's disease (CD) small bowel resection specimens was used to identify potential therapeutic targets for stricturing (S) CD. Using an unbiased approach, 16 cell lineages were assigned within 14,539 sequenced cells from patient-matched SCD and non-stricturing (NSCD) preparations. SCD and NSCD contained identical cell types. Amongst immune cells, B cells and plasma cells were selectively increased in SCD samples. B cell subsets suggested formation of tertiary lymphoid tissue in SCD and compared with NS...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: David T Humphreys Amy Lewis Belen Pan-Castillo Giulio Berti Charles Mein Eva Wozniak Hannah Gordon Radha Gadhok Annamaria Minicozzi Joanna ChinAleong Roger Feakins Eleni Giannoulatou Louisa K James Andrew J Stagg James Oliver Lindsay Andrew Silver Source Type: research
Long non-coding RNA H19 mediates osteogenic differentiation of bone marrow mesenchymal stem cells through the miR-29b-3p/DKK1 axis
This study aimed to explore the molecular mechanism of long non-coding RNA H19 in osteoporosis after SCI and provide new research directions for existing prevention strategies. We used small interfering RNA to knockdown H19 expression and regulated miR-29b-2p expression using miR-29b-3p mimetics and inhibitors. Western blotting, real-time fluorescence quantitative PCR, Alizarin red staining, alkaline phosphatase staining and double-luciferase reporter gene assays were used to assess gene expression, osteogenic ability and binding sites. lncRNA H19 was upregulated in BMSCs from the osteoporosis group, whereas miR-29b-3p was...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Sen Qin Da Liu Source Type: research
Decoding dynamic interactions between EGFR-TKD and DAC through computational and experimental approaches: A novel breakthrough in lung melanoma treatment
This study focuses on CTX-1, exploring its interaction with the pivotal EGFR-TKD protein, a crucial target in lung cancer therapeutics. A meticulous molecular docking analysis revealed that CTX-1 exhibits a noteworthy binding affinity of -7.9 kcal/mol, challenging Erlotinib, a conventional lung cancer medication, which displayed a binding affinity of -7.3 kcal/mol. For a deeper understanding of CTX-1's molecular mechanics, this study employed rigorous 100-ns molecular dynamics simulations, demonstrating CTX-1's remarkable stability in comparison with erlotinib. The Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBS...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Rajesh Kumar Meher Showkat Ahmad Mir Kritika Singh Nobendu Mukerjee Binata Nayak Ajoy Kumer Torki A Zughaibi Mohd Shahnawaz Khan Shams Tabrez Source Type: research
Orexin-A mediates glioblastoma proliferation inhibition by increasing ferroptosis triggered by unstable iron pools and GPX4 depletion
In this report, we provide the first evidence that orexin-A exerts inhibitory effects on GBM proliferation via the induction of ferroptosis. This induction is achieved by instigating an unsustainable increase in iron levels and depletion of GPX4. Moreover, the regulation of TFRC, FTH1 and GPX4 expression through the targeting of NFE2L2 appears to be one of the potential mechanisms underlying orexin-A-induced ferroptosis.PMID:38685674 | PMC:PMC11058333 | DOI:10.1111/jcmm.18318 (Source: J Cell Mol Med)
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Rengzheng Huan Jiqin Zhang Jianhe Yue Sha Yang Guoqiang Han Yuan Cheng Ying Tan Source Type: research
High-fat diet enhances cell proliferation and compromises intestinal permeability in a translational canine intestinal organoid model
CONCLUSIONS: By employing canine intestinal organoid systems, we provide compelling insights into the impact of PA on intestinal physiology. These findings underscore the importance of considering both cell proliferative activity and epithelial integrity in comprehending the repercussions of HFDs on intestinal health. Our study contributes to a deeper understanding of the consequences of HFD on intestinal homeostasis, utilizing valuable translational in vitro models derived from dogs.PMID:38689222 | DOI:10.1186/s12860-024-00512-w (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Itsuma Nagao Yoko M Ambrosini Source Type: research
The modulation of autophagy and unfolded protein response by ent-kaurenoid derivative CPUK02 in human colorectal cancer cells
CONCLUSIONS: The study findings suggest CPUK02 may exert its cytotoxic effects by inhibiting UPR and autophagy flux in colorectal cancer cells.PMID:38689181 | DOI:10.1007/s11033-024-09541-2 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Sedigheh Rezayi Morvarid Siri Nasim Rahmani-Kukia Mozhdeh Zamani Sanaz Dastghaib Pooneh Mokarram Source Type: research
The Role of miR-150-5p/SOCS1 Pathway in Arsenic-Induced Pyroptosis of LX-2 Cells
This study aims to explore the mechanism of pyroptosis of human hepatocyte LX-2 cells induced by NaAsO2 through the miR-150-5p/SOCS1 pathway. LX-2 cells were transfected with different concentrations of NaAsO2, miR-150-5p inhibitor, and SOCS1 agonist. Cell activity, cell pyroptosis, and the expression of related genes and proteins were detected by scanning electron microscopy, CCK-8, qRT-PCR, western blot, and immunofluorescence. Compared with the control group, 10 µmol/L and 20 µmol/L NaAsO2 significantly elevated the protein expression levels of the pyroptosis-related proteins NLRP3, GSDMD, GSDMD-N, caspase1, and cleav...
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Mengyao Zhang Linzhi Li Shugang Li Source Type: research
Single-cell transcriptomics: background, technologies, applications, and challenges
Mol Biol Rep. 2024 Apr 30;51(1):600. doi: 10.1007/s11033-024-09553-y.ABSTRACTSingle-cell sequencing was developed as a high-throughput tool to elucidate unusual and transient cell states that are barely visible in the bulk. This technology reveals the evolutionary status of cells and differences between populations, helps to identify unique cell subtypes and states, reveals regulatory relationships between genes, targets and molecular mechanisms in disease processes, tumor heterogeneity, the state of the immune environment, etc. However, the high cost and technical limitations of single-cell sequencing initially prevented ...
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Lucky Duhan Deepika Kumari Mohammad Naime Virinder S Parmar Anil K Chhillar Mehak Dangi Ritu Pasrija Source Type: research
Exploring the potential of selective oxidation in bioconjugation of collagen with xyloglucan carboxylates
In this study, XG was extracted from Tamarind seeds and subjected to four regioselective oxidation methods to introduce carboxyl groups onto the XG molecules for a bioconjugation with collagen. Galactose oxidase and reducing end aldehyde group oxidation mainly resulted in a low carboxylate content at ∼0.34 mmol/g, whereas the primary and secondary hydroxyl group oxidations would lead to a high carboxyl content at ∼0.84 mmol/g. The number-average molar mass (Mn) and weight-average molar mass (Mw) of XG were 8.8 × 105 g/mol and 1.1 × 106 g/mol, respectively. The oxidized XGs were then subjected to a further biofunction...
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Haixin Jiao Meng Zhang Jianzhong Sun Sameh Samir Ali Hongxing Zhang Yan Li Xiangyu Wang Yinyi Fu Xiulun Wang Jun Liu Source Type: research
A New Methodology for the Oxygen Measurement in Lung Tissue of an Aged Ferret Model Proves Hypoxia During COVID-19
This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/). .PMID:38687560 | DOI:10.1165/rcmb.2024-0005OC (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Katrin Wirz Claudia Schulz Franz S öbbeler Federico Armando Georg Beythien Ingo Gerhauser Nicole de Buhr Veronika Pilchov á Christian Meyer Zu Natrup Wolfgang Baumg ärtner Sabine K ästner Maren von K öckritz-Blickwede Source Type: research
Tumor microenvironment and immune response: A gateway to novel therapies in gastrointestinal cancers
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 28:167203. doi: 10.1016/j.bbadis.2024.167203. Online ahead of print.NO ABSTRACTPMID:38688415 | DOI:10.1016/j.bbadis.2024.167203 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 30, 2024 Category: Molecular Biology Authors: Damian Jacenik Source Type: research
A potassium-chloride co-transporter with altered genome architecture functions as a suppressor in glioma
J Cell Mol Med. 2024 May;28(9):e18352. doi: 10.1111/jcmm.18352.ABSTRACTGliomas, the most lethal tumours in brain, have a poor prognosis despite accepting standard treatment. Limited benefits from current therapies can be attributed to genetic, epigenetic and microenvironmental cues that affect cell programming and drive tumour heterogeneity. Through the analysis of Hi-C data, we identified a potassium-chloride co-transporter SLC12A5 associated with disrupted topologically associating domain which was downregulated in tumour tissues. Multiple independent glioma cohorts were included to analyse the characterization of SLC12A...
Source: J Cell Mol Med - April 30, 2024 Category: Molecular Biology Authors: Hongwei Liu Zhouyang Pan Xuelei Lin Long Chen Qi Yang Wei Zhang Luohuan Dai Yihao Zhang Wang Li Yinhua Chen Kang Peng Siyi Wanggou Feiyue Zeng Xuejun Li Source Type: research
