Early life adversity is associated with differential gene expression in immune cells: A cluster-based analysis across an acute psychosocial stressor
Brain Behav Immun. 2024 Apr 23:S0889-1591(24)00384-2. doi: 10.1016/j.bbi.2024.04.035. Online ahead of print.ABSTRACTElucidating mechanisms by which early-life adversity (ELA) contributes to increased disease risk is important for mitigating adverse health outcomes. Prior work has found differences in immune cell gene expression related to inflammation and mitochondrial activity. Using a within-person between-group experimental design, we investigated differences in gene expression clusters across acute psychosocial stress and no-stress conditions. Participants were young adults (N = 29, aged 18 - 25 years, 62 % female, 47 ...
Source: Brain, Behavior, and Immunity - April 25, 2024 Category: Neurology Authors: Laura Etzel Abner T Apsley Waylon J Hastings Qiaofeng Ye Idan Shalev Source Type: research
A systematic review on the biochemical threshold of mitochondrial genetic variants [REVIEW]
Mitochondrial DNA (mtDNA) variants cause a range of diseases from severe pediatric syndromes to aging-related conditions. The percentage of mtDNA copies carrying a pathogenic variant, variant allele frequency (VAF), must reach a threshold before a biochemical defect occurs, termed the biochemical threshold. Whether the often-cited biochemical threshold of >60% VAF is similar across mtDNA variants and cell types is unclear. In our systematic review, we sought to identify the biochemical threshold of mtDNA variants in relation to VAF by human tissue/cell type. We used controlled vocabulary terms to identify articles measu...
Source: Genome Research - April 25, 2024 Category: Genetics & Stem Cells Authors: Smith, K. K., Moreira, J. D., Wilson, C. R., Padera, J. O., Lamason, A. N., Xue, L., Gopal, D. M., Flynn, D. B., Fetterman, J. L. Tags: REVIEW Source Type: research
A PCR-independent approach for mtDNA enrichment and next-generation sequencing: comprehensive evaluation and clinical application
Sequencing the mitochondrial genome has been increasingly important for the investigation of primary mitochondrial diseases (PMD) and mitochondrial genetics. To overcome the limitations originating from PCR-ba... (Source: Journal of Translational Medicine)
Source: Journal of Translational Medicine - April 25, 2024 Category: Research Authors: Dong Liang, Lin Zhu, Yuqing Zhu, Mingtao Huang, Ying Lin, Hang Li, Ping Hu, Jun Zhang, Bin Shen and Zhengfeng Xu Tags: Methodology Source Type: research
GFRA4 improves the neurogenic potential of enteric neural crest stem cells via hedgehog pathway
CONCLUSION: GFRA4 promotes proliferation and differentiation but inhibits apoptosis of ENCSCs via the hedgehog pathway in HSCR.IMPACT: This study confirms that GFRA4 improves the proliferation and differentiation of ENCSCs via modulation of the hedgehog pathway. This study for the first time revealed the role and the mechanism of the action of GFRA4 in HSCR, which indicates that GFRA4 may play a role in the pathological development of HSCR. Our findings may lay the foundation for further investigation of the mechanisms underlying HSCR development and into targets of HSCR treatment.PMID:38658664 | DOI:10.1038/s41390-024-031...
Source: Cell Research - April 24, 2024 Category: Cytology Authors: Fangfang Zhang Mingyu Cui Lijuan Zhang Bangzhen Ma Feng Guo Gang Wang Source Type: research
Competitive adsorption of microRNA-532-3p by circular RNA SOD2 activates Thioredoxin Interacting Protein/NLR family pyrin domain containing 3 pathway and promotes pyroptosis of non-alcoholic fatty hepatocytes
CONCLUSION: circSOD2 competitively sponges miR-532-3p to activate the TXNIP/NLRP3 inflammasome signaling pathway, promoting pyroptosis in NAFLD.PMID:38659023 | DOI:10.1186/s40001-024-01817-4 (Source: Cell Research)
Source: Cell Research - April 24, 2024 Category: Cytology Authors: FengJuan Chen YuFeng Xing ZhiJie Chen XiaoMan Chen Jie Li Si Gong Fang Luo QingXian Cai Source Type: research
GFRA4 improves the neurogenic potential of enteric neural crest stem cells via hedgehog pathway
CONCLUSION: GFRA4 promotes proliferation and differentiation but inhibits apoptosis of ENCSCs via the hedgehog pathway in HSCR.IMPACT: This study confirms that GFRA4 improves the proliferation and differentiation of ENCSCs via modulation of the hedgehog pathway. This study for the first time revealed the role and the mechanism of the action of GFRA4 in HSCR, which indicates that GFRA4 may play a role in the pathological development of HSCR. Our findings may lay the foundation for further investigation of the mechanisms underlying HSCR development and into targets of HSCR treatment.PMID:38658664 | DOI:10.1038/s41390-024-031...
Source: Pediatric Research - April 24, 2024 Category: Pediatrics Authors: Fangfang Zhang Mingyu Cui Lijuan Zhang Bangzhen Ma Feng Guo Gang Wang Source Type: research
Single-cell analysis revealing the metabolic landscape of prostate cancer
Asian J Androl. 2024 Apr 23. doi: 10.4103/aja20243. Online ahead of print.ABSTRACTTumor metabolic reprogramming is a hallmark of cancer development, and targeting metabolic vulnerabilities has been proven to be an effective approach for castration-resistant prostate cancer (CRPC) treatment. Nevertheless, treatment failure inevitably occurs, largely due to cellular heterogeneity, which cannot be deciphered by traditional bulk sequencing techniques. By employing computational pipelines for single-cell RNA sequencing, we demonstrated that epithelial cells within the prostate are more metabolically active and plastic than stro...
Source: Asian Journal of Andrology - April 24, 2024 Category: Urology & Nephrology Authors: Jing Wang He-Kang Ding Han-Jiang Xu De-Kai Hu William Hankey Li Chen Jun Xiao Chao-Zhao Liang Bing Zhao Ling-Fan Xu Source Type: research
GFRA4 improves the neurogenic potential of enteric neural crest stem cells via hedgehog pathway
CONCLUSION: GFRA4 promotes proliferation and differentiation but inhibits apoptosis of ENCSCs via the hedgehog pathway in HSCR.IMPACT: This study confirms that GFRA4 improves the proliferation and differentiation of ENCSCs via modulation of the hedgehog pathway. This study for the first time revealed the role and the mechanism of the action of GFRA4 in HSCR, which indicates that GFRA4 may play a role in the pathological development of HSCR. Our findings may lay the foundation for further investigation of the mechanisms underlying HSCR development and into targets of HSCR treatment.PMID:38658664 | DOI:10.1038/s41390-024-031...
Source: Pediatric Research - April 24, 2024 Category: Pediatrics Authors: Fangfang Zhang Mingyu Cui Lijuan Zhang Bangzhen Ma Feng Guo Gang Wang Source Type: research
Single-cell analysis revealing the metabolic landscape of prostate cancer
Asian J Androl. 2024 Apr 23. doi: 10.4103/aja20243. Online ahead of print.ABSTRACTTumor metabolic reprogramming is a hallmark of cancer development, and targeting metabolic vulnerabilities has been proven to be an effective approach for castration-resistant prostate cancer (CRPC) treatment. Nevertheless, treatment failure inevitably occurs, largely due to cellular heterogeneity, which cannot be deciphered by traditional bulk sequencing techniques. By employing computational pipelines for single-cell RNA sequencing, we demonstrated that epithelial cells within the prostate are more metabolically active and plastic than stro...
Source: Asian Journal of Andrology - April 24, 2024 Category: Urology & Nephrology Authors: Jing Wang He-Kang Ding Han-Jiang Xu De-Kai Hu William Hankey Li Chen Jun Xiao Chao-Zhao Liang Bing Zhao Ling-Fan Xu Source Type: research
Single-cell analysis revealing the metabolic landscape of prostate cancer
Asian J Androl. 2024 Apr 23. doi: 10.4103/aja20243. Online ahead of print.ABSTRACTTumor metabolic reprogramming is a hallmark of cancer development, and targeting metabolic vulnerabilities has been proven to be an effective approach for castration-resistant prostate cancer (CRPC) treatment. Nevertheless, treatment failure inevitably occurs, largely due to cellular heterogeneity, which cannot be deciphered by traditional bulk sequencing techniques. By employing computational pipelines for single-cell RNA sequencing, we demonstrated that epithelial cells within the prostate are more metabolically active and plastic than stro...
Source: Asian Journal of Andrology - April 24, 2024 Category: Urology & Nephrology Authors: Jing Wang He-Kang Ding Han-Jiang Xu De-Kai Hu William Hankey Li Chen Jun Xiao Chao-Zhao Liang Bing Zhao Ling-Fan Xu Source Type: research
Single-cell analysis revealing the metabolic landscape of prostate cancer
Asian J Androl. 2024 Apr 23. doi: 10.4103/aja20243. Online ahead of print.ABSTRACTTumor metabolic reprogramming is a hallmark of cancer development, and targeting metabolic vulnerabilities has been proven to be an effective approach for castration-resistant prostate cancer (CRPC) treatment. Nevertheless, treatment failure inevitably occurs, largely due to cellular heterogeneity, which cannot be deciphered by traditional bulk sequencing techniques. By employing computational pipelines for single-cell RNA sequencing, we demonstrated that epithelial cells within the prostate are more metabolically active and plastic than stro...
Source: Asian Journal of Andrology - April 24, 2024 Category: Urology & Nephrology Authors: Jing Wang He-Kang Ding Han-Jiang Xu De-Kai Hu William Hankey Li Chen Jun Xiao Chao-Zhao Liang Bing Zhao Ling-Fan Xu Source Type: research
Single-cell analysis revealing the metabolic landscape of prostate cancer
Asian J Androl. 2024 Apr 23. doi: 10.4103/aja20243. Online ahead of print.ABSTRACTTumor metabolic reprogramming is a hallmark of cancer development, and targeting metabolic vulnerabilities has been proven to be an effective approach for castration-resistant prostate cancer (CRPC) treatment. Nevertheless, treatment failure inevitably occurs, largely due to cellular heterogeneity, which cannot be deciphered by traditional bulk sequencing techniques. By employing computational pipelines for single-cell RNA sequencing, we demonstrated that epithelial cells within the prostate are more metabolically active and plastic than stro...
Source: Asian Journal of Andrology - April 24, 2024 Category: Urology & Nephrology Authors: Jing Wang He-Kang Ding Han-Jiang Xu De-Kai Hu William Hankey Li Chen Jun Xiao Chao-Zhao Liang Bing Zhao Ling-Fan Xu Source Type: research
Genes, Vol. 15, Pages 530: Mutations in NSUN3, a Mitochondrial Methyl Transferase Gene, Cause Inherited Optic Neuropathy
In conclusion, pathogenic variants in NSUN3 can cause optic neuropathy. Trio whole-exome sequencing should be considered as a diagnostic strategy in ION cases where standard diagnostic analysis does not reveal disease-causing variants. (Source: Genes)
Source: Genes - April 24, 2024 Category: Genetics & Stem Cells Authors: Cansu de Muijnck Jacoline B. ten Brink Hugoline G. de Haan Richard J. Rodenburg Nicole I. Wolf Arthur A. Bergen Camiel J. F. Boon Maria M. van Genderen Tags: Case Report Source Type: research
Competitive adsorption of microRNA-532-3p by circular RNA SOD2 activates Thioredoxin Interacting Protein/NLR family pyrin domain containing 3 pathway and promotes pyroptosis of non-alcoholic fatty hepatocytes
ConclusioncircSOD2 competitively sponges miR-532-3p to activate the TXNIP/NLRP3 inflammasome signaling pathway, promoting pyroptosis in NAFLD. (Source: European Journal of Medical Research)
Source: European Journal of Medical Research - April 24, 2024 Category: Research Source Type: research
TNF- α signals through ITK-Akt-mTOR to drive CD4 < sup > + < /sup > T cell metabolic reprogramming, which is dysregulated in rheumatoid arthritis
Sci Signal. 2024 Apr 23;17(833):eadg5678. doi: 10.1126/scisignal.adg5678. Epub 2024 Apr 23.ABSTRACTUpon activation, T cells undergo metabolic reprogramming to meet the bioenergetic demands of clonal expansion and effector function. Because dysregulated T cell cytokine production and metabolic phenotypes coexist in chronic inflammatory disease, including rheumatoid arthritis (RA), we investigated whether inflammatory cytokines released by differentiating T cells amplified their metabolic changes. We found that tumor necrosis factor-α (TNF-α) released by human naïve CD4+ T cells upon activation stimulated the expression o...
Source: Science Signaling - April 23, 2024 Category: Biomedical Science Authors: Emma L Bishop Nancy Gudgeon Taylor Fulton-Ward Victoria Stavrou Jennie Roberts Adam Boufersaoui Daniel A Tennant Martin Hewison Karim Raza Sarah Dimeloe Source Type: research
