Neopterin in patients with COPD, asthma, and ACO: association with endothelial and lung functions
CONCLUSION: NP was related to disease severity of chronic airway diseases and involved in the pathogenesis of endothelial dysfunction. A high NP level may contribute to endothelial dysfunction by increasing the oxidative stress of ECs dose-dependently in a hypoxic environment. Our findings may provide a novel evaluation and therapeutic target for endothelial dysfunction related to chronic airway diseases.PMID:38637774 | PMC:PMC11027266 | DOI:10.1186/s12931-024-02784-4 (Source: Respiratory Care)
Source: Respiratory Care - April 18, 2024 Category: Respiratory Medicine Authors: Yangli Liu Fengjia Chen Zhimin Zeng Chengcheng Lei Dubo Chen Xiaoyu Zhang Source Type: research
Histidine tag modified magnetic beads for analysis of arsenic binding proteins
Anal Chim Acta. 2024 May 22;1304:342554. doi: 10.1016/j.aca.2024.342554. Epub 2024 Mar 29.ABSTRACTBACKGROUND: Many proteins with thiol groups can bind with trivalent arsenic which are termed as arsenic binding proteins, thus change their physiological functions. Therefore, it is vital to analyze the arsenic binding proteins in cells. The Pull-Down strategy based on biotinylated phenylarsenic acid (Bio-PAO(III)) probes is an effective way for analysis of arsenic binding proteins. In this strategy, streptavidin magnetic beads (SA-MBs) was applied to capture the arsenic binding proteins conjugating with Bio-PAO(III) probe. Ho...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kai Nan Man He Beibei Chen Bin Hu Source Type: research
Differential Susceptibility to Propofol and Ketamine in Primary Cultures of Young and Senesced Astrocytes
In this study, we examined the survival and viability of both young and senescent astrocytes in culture after adding propofol and ketamine to the media at varying strengths. Oxidative stimulus was applied to commercially available fetal cell lines of human astrocytes in vitro to induce morphological changes in cellular senescence. Our results indicate that propofol reduces the survival of young astrocytes as compared to controls, as well as to ketamine. These effects were seen in comparisons of total cell count and at both high and low dose concentrations. High doses of propofol also significantly reduced cell viability co...
Source: Anesthesiology Research and Practice - April 18, 2024 Category: Anesthesiology Authors: Liang Huang Ferit Tuzer Abigail Murtha Michael Green Claudio Torres Henry Liu Shadi Malaeb Source Type: research
Histidine tag modified magnetic beads for analysis of arsenic binding proteins
Anal Chim Acta. 2024 May 22;1304:342554. doi: 10.1016/j.aca.2024.342554. Epub 2024 Mar 29.ABSTRACTBACKGROUND: Many proteins with thiol groups can bind with trivalent arsenic which are termed as arsenic binding proteins, thus change their physiological functions. Therefore, it is vital to analyze the arsenic binding proteins in cells. The Pull-Down strategy based on biotinylated phenylarsenic acid (Bio-PAO(III)) probes is an effective way for analysis of arsenic binding proteins. In this strategy, streptavidin magnetic beads (SA-MBs) was applied to capture the arsenic binding proteins conjugating with Bio-PAO(III) probe. Ho...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kai Nan Man He Beibei Chen Bin Hu Source Type: research
Differential Susceptibility to Propofol and Ketamine in Primary Cultures of Young and Senesced Astrocytes
In this study, we examined the survival and viability of both young and senescent astrocytes in culture after adding propofol and ketamine to the media at varying strengths. Oxidative stimulus was applied to commercially available fetal cell lines of human astrocytes in vitro to induce morphological changes in cellular senescence. Our results indicate that propofol reduces the survival of young astrocytes as compared to controls, as well as to ketamine. These effects were seen in comparisons of total cell count and at both high and low dose concentrations. High doses of propofol also significantly reduced cell viability co...
Source: Anesthesiology Research and Practice - April 18, 2024 Category: Anesthesiology Authors: Liang Huang Ferit Tuzer Abigail Murtha Michael Green Claudio Torres Henry Liu Shadi Malaeb Source Type: research
Histidine tag modified magnetic beads for analysis of arsenic binding proteins
Anal Chim Acta. 2024 May 22;1304:342554. doi: 10.1016/j.aca.2024.342554. Epub 2024 Mar 29.ABSTRACTBACKGROUND: Many proteins with thiol groups can bind with trivalent arsenic which are termed as arsenic binding proteins, thus change their physiological functions. Therefore, it is vital to analyze the arsenic binding proteins in cells. The Pull-Down strategy based on biotinylated phenylarsenic acid (Bio-PAO(III)) probes is an effective way for analysis of arsenic binding proteins. In this strategy, streptavidin magnetic beads (SA-MBs) was applied to capture the arsenic binding proteins conjugating with Bio-PAO(III) probe. Ho...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kai Nan Man He Beibei Chen Bin Hu Source Type: research
Differential Susceptibility to Propofol and Ketamine in Primary Cultures of Young and Senesced Astrocytes
In this study, we examined the survival and viability of both young and senescent astrocytes in culture after adding propofol and ketamine to the media at varying strengths. Oxidative stimulus was applied to commercially available fetal cell lines of human astrocytes in vitro to induce morphological changes in cellular senescence. Our results indicate that propofol reduces the survival of young astrocytes as compared to controls, as well as to ketamine. These effects were seen in comparisons of total cell count and at both high and low dose concentrations. High doses of propofol also significantly reduced cell viability co...
Source: Anesthesiology Research and Practice - April 18, 2024 Category: Anesthesiology Authors: Liang Huang Ferit Tuzer Abigail Murtha Michael Green Claudio Torres Henry Liu Shadi Malaeb Source Type: research
Histidine tag modified magnetic beads for analysis of arsenic binding proteins
Anal Chim Acta. 2024 May 22;1304:342554. doi: 10.1016/j.aca.2024.342554. Epub 2024 Mar 29.ABSTRACTBACKGROUND: Many proteins with thiol groups can bind with trivalent arsenic which are termed as arsenic binding proteins, thus change their physiological functions. Therefore, it is vital to analyze the arsenic binding proteins in cells. The Pull-Down strategy based on biotinylated phenylarsenic acid (Bio-PAO(III)) probes is an effective way for analysis of arsenic binding proteins. In this strategy, streptavidin magnetic beads (SA-MBs) was applied to capture the arsenic binding proteins conjugating with Bio-PAO(III) probe. Ho...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kai Nan Man He Beibei Chen Bin Hu Source Type: research
Differential Susceptibility to Propofol and Ketamine in Primary Cultures of Young and Senesced Astrocytes
In this study, we examined the survival and viability of both young and senescent astrocytes in culture after adding propofol and ketamine to the media at varying strengths. Oxidative stimulus was applied to commercially available fetal cell lines of human astrocytes in vitro to induce morphological changes in cellular senescence. Our results indicate that propofol reduces the survival of young astrocytes as compared to controls, as well as to ketamine. These effects were seen in comparisons of total cell count and at both high and low dose concentrations. High doses of propofol also significantly reduced cell viability co...
Source: Anesthesiology Research and Practice - April 18, 2024 Category: Anesthesiology Authors: Liang Huang Ferit Tuzer Abigail Murtha Michael Green Claudio Torres Henry Liu Shadi Malaeb Source Type: research
Exploring the role of Prx II in mitigating endoplasmic reticulum stress and mitochondrial dysfunction in neurodegeneration
Neurodegenerative diseases are increasingly recognized for their association with oxidative stress, which leads to progressive dysfunction and loss of neurons, manifesting in cognitive and motor impairments. T... (Source: Cell Communication and Signaling)
Source: Cell Communication and Signaling - April 18, 2024 Category: Molecular Biology Authors: Mei-Hua Jin, Lin Feng, Hong-Yi Xiang, Hu-Nan Sun, Ying-Hao Han and Taeho Kwon Tags: Research Source Type: research
Molecules, Vol. 29, Pages 1848: Advanced Nano-Drug Delivery Systems in the Treatment of Ischemic Stroke
Chen
In recent years, the frequency of strokes has been on the rise year by year and has become the second leading cause of death around the world, which is characterized by a high mortality rate, high recurrence rate, and high disability rate. Ischemic strokes account for a large percentage of strokes. A reperfusion injury in ischemic strokes is a complex cascade of oxidative stress, neuroinflammation, immune infiltration, and mitochondrial damage. Conventional treatments are ineffective, and the presence of the blood–brain barrier (BBB) leads to inefficient drug delivery utilization, so researchers are...
Source: Molecules - April 18, 2024 Category: Chemistry Authors: Jiajie Zhang Zhong Chen Qi Chen Tags: Review Source Type: research
Mini-encyclopedia of mitochondria-relevant nutraceuticals protecting health in primary and secondary care —clinically relevant 3PM innovation
AbstractDespite their subordination in humans, to a great extent, mitochondria maintain their independent status but tightly cooperate with the “host” on protecting the joint life quality and minimizing health risks. Under oxidative stress conditions, healthy mitochondria promptly increase mitophagy level to remove damaged “fellows” rejuvenating the mitochondrial population and sending fragments of mtDNA as SOS signals to all system s in the human body. As long as metabolic pathways are under systemic control and well-concerted together, adaptive mechanisms become triggered increasing systemic protection, activatin...
Source: EPMA Journal - April 18, 2024 Category: International Medicine & Public Health Source Type: research
FoxG1 as a Potential Therapeutic Target for Alzheimer's Disease: Modulating NLRP3 Inflammasome via AMPK/mTOR Autophagy Pathway
Cell Mol Neurobiol. 2024 Apr 17;44(1):35. doi: 10.1007/s10571-024-01467-4.ABSTRACTAn increasing body of research suggests that promoting microglial autophagy hinders the neuroinflammation initiated though the NLRP3 inflammasome activation in Alzheimer's disease (AD). The function of FoxG1, a crucial transcription factor involved in cell survival by regulating mitochondrial function, remains unknown during the AD process and neuroinflammation occurs. In the present study, we firstly found that Aβ peptides induced AD-like neuroinflammation upregulation and downregulated the level of autophagy. Following low-dose Aβ25-35 st...
Source: Cellular and Molecular Neurobiology - April 17, 2024 Category: Cytology Authors: Qi Yun Si-Fei Ma Wei-Ning Zhang Meng Gu Jia Wang Source Type: research
Calorie restriction anti-hypertrophic effects are associated with improved mitochondrial content, blockage of Ca < sup > 2+ < /sup > -induced mitochondrial damage, and lower reverse electron transport-mediated oxidative stress
In this study, we show that this dietetic intervention prevents cardiac protein elevation, avoids fetal gene reprogramming (atrial natriuretic peptide), and blocks the increase in heart weight per tibia length index (HW/TL) seen in isoproterenol-induced cardiac hypertrophy. Our findings suggest that calorie restriction inhibits cardiac pathological growth while also lowering mitochondrial reverse electron transport-induced hydrogen peroxide formation and improving mitochondrial content. Calorie restriction also attenuated the opening of the Ca2+-induced mitochondrial permeability transition pore. We also found that calorie...
Source: Free Radical Research - April 17, 2024 Category: Research Authors: Aline Maria Brito Lucas Plinio Bezerra Palacio Pedro Lourenzo Oliveira Cunha Heberty Tarso Facundo Source Type: research
