Soybean lecithin and cholesterol-loaded cyclodextrin in combination to enhances the cryosurvival of dairy goat semen
The objective of the study was to examine the effect of soy lecithin (SL) and cholesterol loaded cryclodestrin (CLC) on cryo-survival of sperm cryopreserved in the presence or absence of seminal plasma in Saanen dairy goats. Tris-based dilutions containing various concentrations of SL (0, 0.5%, 1.0% or 2.0%) and CLC (0, 2.0 g/L, 4.0 g/L or 6.0 g/L CLC) were used to cryopreserve Saanen dairy goat sperm. The quality of frozen-thawed sperm, including progressive motility, viability, acrosome and plasma membrane integrity, as well as fertility were detected. Results found that the optimal combination of the two cryoprotectants...
Source: Cryobiology - July 14, 2023 Category: Biology Authors: Rong Li Hubei Zhao Bowen Li Siqi Wang Song Hua Source Type: research
Soybean lecithin and cholesterol-loaded cyclodextrin in combination to enhances the cryosurvival of dairy goat semen
The objective of the study was to examine the effect of soy lecithin (SL) and cholesterol loaded cryclodestrin (CLC) on cryo-survival of sperm cryopreserved in the presence or absence of seminal plasma in Saanen dairy goats. Tris-based dilutions containing various concentrations of SL (0, 0.5%, 1.0% or 2.0%) and CLC (0, 2.0 g/L, 4.0 g/L or 6.0 g/L CLC) were used to cryopreserve Saanen dairy goat sperm. The quality of frozen-thawed sperm, including progressive motility, viability, acrosome and plasma membrane integrity, as well as fertility were detected. Results found that the optimal combination of the two cryoprotectants...
Source: Cryobiology - July 14, 2023 Category: Biology Authors: Rong Li Hubei Zhao Bowen Li Siqi Wang Song Hua Source Type: research
Soybean lecithin and cholesterol-loaded cyclodextrin in combination to enhances the cryosurvival of dairy goat semen
The objective of the study was to examine the effect of soy lecithin (SL) and cholesterol loaded cryclodestrin (CLC) on cryo-survival of sperm cryopreserved in the presence or absence of seminal plasma in Saanen dairy goats. Tris-based dilutions containing various concentrations of SL (0, 0.5%, 1.0% or 2.0%) and CLC (0, 2.0 g/L, 4.0 g/L or 6.0 g/L CLC) were used to cryopreserve Saanen dairy goat sperm. The quality of frozen-thawed sperm, including progressive motility, viability, acrosome and plasma membrane integrity, as well as fertility were detected. Results found that the optimal combination of the two cryoprotectants...
Source: Cryobiology - July 14, 2023 Category: Biology Authors: Rong Li Hubei Zhao Bowen Li Siqi Wang Song Hua Source Type: research
Soybean lecithin and cholesterol-loaded cyclodextrin in combination to enhances the cryosurvival of dairy goat semen
The objective of the study was to examine the effect of soy lecithin (SL) and cholesterol loaded cryclodestrin (CLC) on cryo-survival of sperm cryopreserved in the presence or absence of seminal plasma in Saanen dairy goats. Tris-based dilutions containing various concentrations of SL (0, 0.5%, 1.0% or 2.0%) and CLC (0, 2.0 g/L, 4.0 g/L or 6.0 g/L CLC) were used to cryopreserve Saanen dairy goat sperm. The quality of frozen-thawed sperm, including progressive motility, viability, acrosome and plasma membrane integrity, as well as fertility were detected. Results found that the optimal combination of the two cryoprotectants...
Source: Cryobiology - July 14, 2023 Category: Biology Authors: Rong Li Hubei Zhao Bowen Li Siqi Wang Song Hua Source Type: research
Development of κ-carrageenan-PEG/lecithin bioactive hydrogel membranes for antibacterial adhesion and painless detachment
Int J Biol Macromol. 2023 Jul 10:125789. doi: 10.1016/j.ijbiomac.2023.125789. Online ahead of print.ABSTRACTThe issue of wound dressing adherence poses a substantial challenge in the field of wound care, with implications both clinically and economically. Overcoming this challenge requires the development of a hydrogel dressing that enables painless removal without causing any secondary damage. However, addressing this issue still remains a significant challenge that requires attention and further exploration. The present study is focused on the synthesis of hydrogel membranes based on κ-carrageenan (CG), polyethylene gly...
Source: International Journal of Biological Macromolecules - July 12, 2023 Category: Biochemistry Authors: Pratibha Singh Chetna Verma Amlan Gupta Samrat Mukhopadhyay Bhuvanesh Gupta Source Type: research
Development of κ-carrageenan-PEG/lecithin bioactive hydrogel membranes for antibacterial adhesion and painless detachment
Int J Biol Macromol. 2023 Jul 10:125789. doi: 10.1016/j.ijbiomac.2023.125789. Online ahead of print.ABSTRACTThe issue of wound dressing adherence poses a substantial challenge in the field of wound care, with implications both clinically and economically. Overcoming this challenge requires the development of a hydrogel dressing that enables painless removal without causing any secondary damage. However, addressing this issue still remains a significant challenge that requires attention and further exploration. The present study is focused on the synthesis of hydrogel membranes based on κ-carrageenan (CG), polyethylene gly...
Source: International Journal of Biological Macromolecules - July 12, 2023 Category: Biochemistry Authors: Pratibha Singh Chetna Verma Amlan Gupta Samrat Mukhopadhyay Bhuvanesh Gupta Source Type: research
Development of κ-carrageenan-PEG/lecithin bioactive hydrogel membranes for antibacterial adhesion and painless detachment
Int J Biol Macromol. 2023 Jul 10:125789. doi: 10.1016/j.ijbiomac.2023.125789. Online ahead of print.ABSTRACTThe issue of wound dressing adherence poses a substantial challenge in the field of wound care, with implications both clinically and economically. Overcoming this challenge requires the development of a hydrogel dressing that enables painless removal without causing any secondary damage. However, addressing this issue still remains a significant challenge that requires attention and further exploration. The present study is focused on the synthesis of hydrogel membranes based on κ-carrageenan (CG), polyethylene gly...
Source: International Journal of Biological Macromolecules - July 12, 2023 Category: Biochemistry Authors: Pratibha Singh Chetna Verma Amlan Gupta Samrat Mukhopadhyay Bhuvanesh Gupta Source Type: research
Exploring the use of modified in vitro digestion assays for the evaluation of ritonavir loaded solid lipid-based formulations
In this study, the suitability of three different in vitro digestion assays for the evaluation of sLBFs was assessed; the classic one-step intestinal digestion assay, a two-step gastrointestinal digestion assay and a bicompartmental assay permitting the simultaneous monitoring of digestion and permeation of the active pharmaceutical ingredient (API) across an artificial membrane (Lecithin in Dodecane - LiDo). Three sLBFs (M1-M3) with varied composition and ritonavir as model drug were prepared and examined. When comparing the ability of these formulations to keep the drug solubilized in the aqueous phase, all three assays ...
Source: European Journal of Pharmaceutical Sciences - July 11, 2023 Category: Drugs & Pharmacology Authors: Ioannis I Andreadis Arne Schulzen Julian Quodbach Christel A S Bergstr öm Source Type: research
Exploring the use of modified in vitro digestion assays for the evaluation of ritonavir loaded solid lipid-based formulations
In this study, the suitability of three different in vitro digestion assays for the evaluation of sLBFs was assessed; the classic one-step intestinal digestion assay, a two-step gastrointestinal digestion assay and a bicompartmental assay permitting the simultaneous monitoring of digestion and permeation of the active pharmaceutical ingredient (API) across an artificial membrane (Lecithin in Dodecane - LiDo). Three sLBFs (M1-M3) with varied composition and ritonavir as model drug were prepared and examined. When comparing the ability of these formulations to keep the drug solubilized in the aqueous phase, all three assays ...
Source: European Journal of Pharmaceutical Sciences - July 11, 2023 Category: Drugs & Pharmacology Authors: Ioannis I Andreadis Arne Schulzen Julian Quodbach Christel A S Bergstr öm Source Type: research
Self-assembled Gallic acid loaded lecithin-chitosan hybrid nanostructured gel as a potential tool against imiquimod-induced psoriasis
Environ Res. 2023 Jul 5:116562. doi: 10.1016/j.envres.2023.116562. Online ahead of print.ABSTRACTIncreased thickness of the skin and hyperproliferation of keratinocyte cell is the main obstacle in the treatment of psoriasis. Gallic Acid (GA) has shown efficacious results against the hyperproliferation of keratinocytes while lipid-polymer loaded hybrid nanoparticles (LPHNs) have an edge over lipidic and polymeric nanoparticles considering drug loading, controlled release, stability, and retention. The LPHNs were optimized using Box-Behnken method and was further characterized by FTIR, DSC and Zetasizer. The optimized prepar...
Source: Environmental Research - July 7, 2023 Category: Environmental Health Authors: Sahim Aziz Hazari Afsana Sheikh Mohammed A S Abourehab Alaa S Tulbah Prashant Kesharwani Source Type: research
Self-assembled Gallic acid loaded lecithin-chitosan hybrid nanostructured gel as a potential tool against imiquimod-induced psoriasis
Environ Res. 2023 Jul 5:116562. doi: 10.1016/j.envres.2023.116562. Online ahead of print.ABSTRACTIncreased thickness of the skin and hyperproliferation of keratinocyte cell is the main obstacle in the treatment of psoriasis. Gallic Acid (GA) has shown efficacious results against the hyperproliferation of keratinocytes while lipid-polymer loaded hybrid nanoparticles (LPHNs) have an edge over lipidic and polymeric nanoparticles considering drug loading, controlled release, stability, and retention. The LPHNs were optimized using Box-Behnken method and was further characterized by FTIR, DSC and Zetasizer. The optimized prepar...
Source: Cell Research - July 7, 2023 Category: Cytology Authors: Sahim Aziz Hazari Afsana Sheikh Mohammed A S Abourehab Alaa S Tulbah Prashant Kesharwani Source Type: research
Self-assembled Gallic acid loaded lecithin-chitosan hybrid nanostructured gel as a potential tool against imiquimod-induced psoriasis
Environ Res. 2023 Jul 5;234:116562. doi: 10.1016/j.envres.2023.116562. Online ahead of print.ABSTRACTIncreased thickness of the skin and hyperproliferation of keratinocyte cell is the main obstacle in the treatment of psoriasis. Gallic Acid (GA) has shown efficacious results against the hyperproliferation of keratinocytes while lipid-polymer loaded hybrid nanoparticles (LPHNs) have an edge over lipidic and polymeric nanoparticles considering drug loading, controlled release, stability, and retention. The LPHNs were optimized using Box-Behnken method and was further characterized by FTIR, DSC and Zetasizer. The optimized pr...
Source: Environmental Research - July 7, 2023 Category: Environmental Health Authors: Sahim Aziz Hazari Afsana Sheikh Mohammed A S Abourehab Alaa S Tulbah Prashant Kesharwani Source Type: research
Familial LCAT (Lecithin: Cholesterol Acyltransferase) Deficiency: A Case Report
Familial LCAT deficiency (FLD) is a rare genetic disorder for which there is currentlyno treatment. It is marked by extremely reduced HDL-C levels since birth. Corneal opacity is often the first clinical sign of disease and progressive chronic kidney disease develops by the fourth decade of life. While the biochemical aspects of FLD are well understood, little is known about its natural history, hampering the identification of biomarkers which could aid the timely diagnosis of the disease and the development of effective treatments. (Source: Journal of Clinical Lipidology)
Source: Journal of Clinical Lipidology - July 1, 2023 Category: Lipidology Authors: Gregory Alfaro, Jay Pendyala, Archna Bajaj, Cecelia Vitali, Michael E Sulewski, Marina Cuchel Tags: Genetic Disorders Source Type: research
Lipoprotein X - In Graft vs. Host Disease
Lipoprotein-X (LpX) is an abnormal lipoprotein rich in phospholipid and unesterified cholesterol (UC) found in patients with cholestatic liver disease, lecithin-cholesterol acyl transferase (LCAT) deficiency, and after lipid emulsion infusion. LpX has been detected in patients with graft-versus-host disease (GVHD) of the liver. Cholestasis leads to the accumulation of bile salts and UC into the circulation, promoting the formation of LpX. This is often not well-recognized, partly because the detection of LpX is technically challenging and requires specialized laboratory assays. (Source: Journal of Clinical Lipidology)
Source: Journal of Clinical Lipidology - July 1, 2023 Category: Lipidology Authors: Deepak Vedamurthy, Cecilia Vitali, Marina Cuchel, Daniel Soffer, Daniel Rader Tags: Complex or Unusual Cases in Lipid Management Source Type: research
Potential of curcumin and niacin-loaded targeted chitosan coated liposomes to activate autophagy in hepatocellular carcinoma cells: An in vitro evaluation in HePG2 cell line
The objective of this study is to activate autophagy in hepatocellular carcinoma for the enhancement of its cellular degradation. Liposomes incorporated chitosan in the core used to improve the stability of lecithin and increase the niacin loading efficiency. Additionally, curcumin as a hydrophobic molecule entrapped into liposomal layers and used as a face layer to minimize the release of niacin in physiological pH 7.2. Folic acid-conjugated chitosan was used to facilitate the delivery of liposomes into a specific location of cancer cells. TEM, UV Visible spectrophotometer, and FTIR confirmed the successful liposomal form...
Source: International Journal of Biological Macromolecules - June 29, 2023 Category: Biochemistry Authors: Nemany A N Hanafy Rehab Fouad Sheashaa Eman A Moussa Magdy E Mahfouz Source Type: research
