Abnormal expression of CUX1 influences autophagy activation in paroxysmal nocturnal hemoglobinuria
J Leukoc Biol. 2024 Feb 5:qiae008. doi: 10.1093/jleuko/qiae008. Online ahead of print.ABSTRACTThe mechanism underlying autophagy in paroxysmal nocturnal hemoglobinuria (PNH) remains largely unknown. We previously sequenced the entire genome exon of the CD59- cells from 13 patients with PNH and found genes such as CUX1 encoding Cut-like homeobox 1. Peripheral blood samples from nine patients with PNH and seven healthy controls were obtained to measure CUX1 expression. The correlation between CUX1 mRNA expression and PNH clinical indicators was analyzed. To simulate CUX1 expression in patients with PNH, we generated a panel ...
Source: Journal of Leukocyte Biology - February 5, 2024 Category: Hematology Authors: Junshu Wu Liyan Li Zhaoyun Liu Honglei Wang Yingying Chen Lijie Zeng Guanrou Wang Hui Liu Rong Fu Source Type: research
Cultivation of recombinant Aspergillus niger strains on dairy whey as a carbohydrate source
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae007. doi: 10.1093/jimb/kuae007.ABSTRACTAgricultural waste valorisation provides a sustainable solution to waste management, and combining waste utilisation with commodity production allows for responsible production processes. Recombinant Aspergillus niger D15 strains expressing fungal endoglucanases (Trichoderma reesei eg1 and eg2 and Aspergillus carneus aceg) were evaluated for their ability to utilise lactose as a carbon source to determine whether dairy waste could be used as a feedstock for enzyme production. The recombinant A. niger D15[eg1]PyrG, D15[eg2]PyrG, and D15[ace...
Source: Journal of Industrial Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Teagan C Crament Kayline Arendsen Shaunita H Rose Trudy Jansen Source Type: research
Cultivation of recombinant Aspergillus niger strains on dairy whey as a carbohydrate source
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae007. doi: 10.1093/jimb/kuae007.ABSTRACTAgricultural waste valorisation provides a sustainable solution to waste management, and combining waste utilisation with commodity production allows for responsible production processes. Recombinant Aspergillus niger D15 strains expressing fungal endoglucanases (Trichoderma reesei eg1 and eg2 and Aspergillus carneus aceg) were evaluated for their ability to utilise lactose as a carbon source to determine whether dairy waste could be used as a feedstock for enzyme production. The recombinant A. niger D15[eg1]PyrG, D15[eg2]PyrG, and D15[ace...
Source: Journal of Industrial Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Teagan C Crament Kayline Arendsen Shaunita H Rose Trudy Jansen Source Type: research
Cultivation of recombinant Aspergillus niger strains on dairy whey as a carbohydrate source
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae007. doi: 10.1093/jimb/kuae007.ABSTRACTAgricultural waste valorisation provides a sustainable solution to waste management, and combining waste utilisation with commodity production allows for responsible production processes. Recombinant Aspergillus niger D15 strains expressing fungal endoglucanases (Trichoderma reesei eg1 and eg2 and Aspergillus carneus aceg) were evaluated for their ability to utilise lactose as a carbon source to determine whether dairy waste could be used as a feedstock for enzyme production. The recombinant A. niger D15[eg1]PyrG, D15[eg2]PyrG, and D15[ace...
Source: Journal of Industrial Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Teagan C Crament Kayline Arendsen Shaunita H Rose Trudy Jansen Source Type: research
Genomic and phenotypic characterisation of Enterococcus mundtii AM_AQ_BC8 for its anti-biofilm, antimicrobial and probiotic potential
Arch Microbiol. 2024 Jan 31;206(2):84. doi: 10.1007/s00203-023-03816-1.ABSTRACTEnterococcus mundtii AM_AQ_BC8 isolated from biofouled filtration membrane was characterised as a potential probiotic bacterium showing strong L-lactic acid-producing capability. Experimental studies revealed that E. mundtii AM_AQ_BC8 possess antibiofilm and antimicrobial ability too, as tested against strong biofilm-forming bacteria like Pseudomonas spp. The present study has evaluated the genetic potential of E. mundtii AM_AQ_BC8 through genome sequencing. Whole genome analysis revealed the presence of key genes like ldh_1 and ldh_2 responsibl...
Source: Archives of Microbiology - January 31, 2024 Category: Microbiology Authors: Anuja Maitreya Asifa Qureshi Source Type: research
Aloe-emodin exhibits growth-suppressive effects on androgen-independent human prostate cancer DU145 cells via inhibiting the Wnt/ β-catenin signaling pathway: an in vitro and in silico study
At the molecular level, several developmental signaling pathways, such as Wnt/β-catenin, have been associated with the initiation and subsequent progression of prostate carcinomas. The present report elucidated the anti-cancerous attributes of an anthraquinone, aloe-emodin (AE), against androgen-independent human prostate cancer DU145 cells. The cytotoxicity profiling of AE showed that it exerted significant cytotoxic effects and increased lactose dehydrogenase levels in DU145 cells (p < 0.01 and p < 0.001). AE also induced considerable reactive oxygen species (ROS)-mediated oxidative stress, which escalated at high...
Source: Frontiers in Pharmacology - January 29, 2024 Category: Drugs & Pharmacology Source Type: research
Synthesis and Characterization of Baicalein-loaded Aquasomes: An In vitro and In silico Perspective for Diabetes Mellitus
CONCLUSION: Aquasomes offer an alternative approach to conventional delivery methods. The selfassembling characteristics of aquasomes greatly simplify their preparation process, adding to their appeal as a drug delivery system.PMID:38279722 | DOI:10.2174/0115701638263815231226171108 (Source: Current Drug Discovery Technologies)
Source: Current Drug Discovery Technologies - January 27, 2024 Category: Drugs & Pharmacology Authors: Vinay Goyal Bhavna Kumar Diwya Kumar Lal Poorvi Varshney Vijay Singh Rana Source Type: research
Oral treatment of human gut microbiota associated IL-10 −/− mice suffering from acute campylobacteriosis with carvacrol, deferoxamine, deoxycholic acid, and 2-fucosyl-lactose
In conclusion, carvacrol alone and its combination with deferoxamine, deoxycholate, and 2-fucosyl-lactose constitute promising antibiotics-independent treatment options to fight acute campylobacteriosis. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - January 25, 2024 Category: Microbiology Source Type: research
Radio frequency inactivation of Salmonella Typhimurium and Listeria monocytogenes in skimmed and whole milk powder
This study investigates the RF inactivation of Salmonella Typhimurium and Listeria monocytogenes in two types of milk powder; skimmed and whole milk powder. Specifically, the aims were to (i) examine the influence of the powder's composition on bacterial inactivation, (ii) evaluate the response of bacteria with different Gram properties (Gram positive and Gram negative) and (iii) verify the use of Enterococcus faecium as a surrogate for the two microorganisms for the specific RF process. In order to examine exclusively the influence of RF, a non-isothermal temperature profile was used, employing solely different RF energy ...
Source: International Journal of Food Microbiology - January 20, 2024 Category: Food Science Authors: Maria Tonti Davy Verheyen Dmytro Kozak Torstein Sk åra Jan F M Van Impe Source Type: research
The microbial and metabolite composition of Gouda cheese made from pasteurized milk is determined by the processing chain
Int J Food Microbiol. 2024 Feb 16;412:110557. doi: 10.1016/j.ijfoodmicro.2024.110557. Epub 2024 Jan 5.ABSTRACTGouda cheeses of different production batches and ripening times often differ in metabolite composition, which may be due to the starter culture mixture applied or the growth of non-starter lactic acid bacteria (NSLAB) upon maturation. Therefore, a single Gouda cheese production batch was systematically investigated from the thermized milk to the mature cheeses, ripened for up to 100 weeks, to identify the main bacterial species and metabolites and their dynamics during the whole production and ripening. As this se...
Source: International Journal of Food Microbiology - January 18, 2024 Category: Food Science Authors: Hannes Decadt Stefan Weckx Luc De Vuyst Source Type: research
Report of an Iranian child with chronic abdominal pain and constipation diagnosed as glycogen storage disease type IX: a case report
ConclusionGiven the mild and varied clinical manifestations of glycogen storage disease type IX, it is possible for the diagnosis to be overlooked. It is important to consider glycogen storage disease type IX in children who present with unexplained hepatomegaly and elevated transaminase levels. Furthermore, due to the distinct management of glycogen storage disease type IX compared with glycogen storage disease type I and glycogen storage disease type III, genetic analysis is essential for an accurate diagnosis. (Source: Journal of Medical Case Reports)
Source: Journal of Medical Case Reports - January 12, 2024 Category: General Medicine Source Type: research
Effects of biotin and coated cobalamin on lactation performance, nutrient digestion and rumen fermentation in Holstein dairy cows
AbstractBiotin (BI) and cobalamin (CA) are essential for rumen propionate production and hepatic gluconeogenesis. The study evaluated the influence of BI or/and coated CA (CCA) on milk performance and nutrient digestion in cows. Sixty Holstein dairy cows were assigned in a 2 × 2 factorial arrangement and randomised block design to four groups. The factors were BI at 0 or 20 mg/day and CCA at 0 or 9 mg CA/day. Dry matter intake increased with BI addition but was unchanged with CCA supply. Addition of BI or CCA increased fat-corrected milk, milk fat and milk pro tein yields and feed efficiency. Moreover, lactose yi...
Source: Journal of Animal Physiology and Animal Nutrition - January 11, 2024 Category: Zoology Authors: Cong Wang,
Jing An,
LiJun Bu,
YaPeng Liu,
Qiang Liu,
Gang Guo,
Jing Zhang,
YaWei Zhang Tags: ORIGINAL ARTICLE Source Type: research
A novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodology
The objective of this study is to explore the probiotic properties and optimal growth conditions ofLactiplantibacillus plantarum BG24.L. plantarum BG24 exhibited a remarkable ability to utilize lactose, and to grow under acidic conditions and in the presence of high levels of bile salts. The strain showed the highest antibacterial activity againstL. monocytogenes Scott A (zone of inhibition: 26 mm).L. plantarum BG24 was found to be resistant to 8 of the tested 19 antibiotics using the disc diffusion method.and its multiple antibiotic resistance (MAR) index was calculated as 0.421. The adhesion rate to human intestinal ep...
Source: World Journal of Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Source Type: research
Correlating sugar transporter expression and activities to identify transporters for an orphan sugar substrate
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-18. doi: 10.1007/s00253-023-12907-4. Epub 2024 Jan 8.ABSTRACTFilamentous fungi like Neurospora crassa are able to take up and metabolize important sugars present, for example, in agricultural and human food wastes. However, only a fraction of all putative sugar transporters in filamentous fungi has been characterized to date, and for many sugar substrates, the corresponding transporters are unknown. In N. crassa, only 14 out of the 42 putative major facilitator superfamily (MFS)-type sugar transporters have been characterized so far. To uncover this hidden potential for biotechn...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Elisabeth Tamayo Basant Nada Isabell Hafermann J Philipp Benz Source Type: research
