Metabolically engineer Clostridium saccharoperbutylacetonicum for comprehensive conversion of acid whey into valuable biofuels and biochemicals
This study achieves the highest reported butanol titer and yield using acid whey as substrate in clostridia and marks pioneering production of esters using acid whey. The findings demonstrate an innovative bioprocess that enhances renewable feedstock biotransformation, thereby promoting economic viability and environmental sustainability of biomanufacturing.PMID:38554761 | DOI:10.1016/j.biortech.2024.130640 (Source: Bioresource Technology)
Source: Bioresource Technology - March 30, 2024 Category: Biotechnology Authors: Yuechao Ma Na Guo Shangjun Wang Yifen Wang Zhihua Jiang Liang Guo Wei Luo Yi Wang Source Type: research
Metabolically engineer Clostridium saccharoperbutylacetonicum for comprehensive conversion of acid whey into valuable biofuels and biochemicals
This study achieves the highest reported butanol titer and yield using acid whey as substrate in clostridia and marks pioneering production of esters using acid whey. The findings demonstrate an innovative bioprocess that enhances renewable feedstock biotransformation, thereby promoting economic viability and environmental sustainability of biomanufacturing.PMID:38554761 | DOI:10.1016/j.biortech.2024.130640 (Source: Bioresource Technology)
Source: Bioresource Technology - March 30, 2024 Category: Biotechnology Authors: Yuechao Ma Na Guo Shangjun Wang Yifen Wang Zhihua Jiang Liang Guo Wei Luo Yi Wang Source Type: research
Combining thermosonication microstress and pineapple peel extract addition to achieve quality and post-acidification control in yogurt fermentation
This study confirmed the feasibility of thermosonication-preconditioned LAB inocula, in combination with the use of natural active components from fruit processing byproducts, to alleviate post-acidification in yogurt and to enhance its antioxidant activities as well as simultaneously maintaining sensory features.PMID:38552299 | DOI:10.1016/j.ultsonch.2024.106857 (Source: Ultrasonics Sonochemistry)
Source: Ultrasonics Sonochemistry - March 29, 2024 Category: Chemistry Authors: Xiaohui Zhang Yuanrong Zheng Changyu Zhou Jinxuan Cao Yifeng Zhang Zhen Wu Daodong Pan Zhendong Cai Qiang Xia Source Type: research
Cold-induced damage in boar sperm cooled to 5 °C in Lactose Egg Yolk (LEY) medium with different Glycerol concentrations
Anim Reprod Sci. 2024 Mar 13;264:107455. doi: 10.1016/j.anireprosci.2024.107455. Online ahead of print.ABSTRACTBoar sperm is highly susceptible to cold damage. When temperature drops to 5°C, the plasmatic membrane is destabilized. The freezing process causes a reduction of the fertility window because frozen/thawed boar sperm has less survivability. The aim of this work was to analyze the effect on sperm characteristics and response to capacitation stimuli of cooling to 5°C using a controlled protocol. Also, we evaluated if the addition of Glycerol 2% or 3% at 5°C was able to modify these parameters. For this purpose, w...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: Pablo Torres Daniela Marina Malcervelli Jonathan Hern án Cartelle Mar ía Laura Fischman Humberto Osvaldo Cisale Source Type: research
Cold-induced damage in boar sperm cooled to 5 °C in Lactose Egg Yolk (LEY) medium with different Glycerol concentrations
Anim Reprod Sci. 2024 Mar 13;264:107455. doi: 10.1016/j.anireprosci.2024.107455. Online ahead of print.ABSTRACTBoar sperm is highly susceptible to cold damage. When temperature drops to 5°C, the plasmatic membrane is destabilized. The freezing process causes a reduction of the fertility window because frozen/thawed boar sperm has less survivability. The aim of this work was to analyze the effect on sperm characteristics and response to capacitation stimuli of cooling to 5°C using a controlled protocol. Also, we evaluated if the addition of Glycerol 2% or 3% at 5°C was able to modify these parameters. For this purpose, w...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: Pablo Torres Daniela Marina Malcervelli Jonathan Hern án Cartelle Mar ía Laura Fischman Humberto Osvaldo Cisale Source Type: research
Cold-induced damage in boar sperm cooled to 5 °C in Lactose Egg Yolk (LEY) medium with different Glycerol concentrations
Anim Reprod Sci. 2024 Mar 13;264:107455. doi: 10.1016/j.anireprosci.2024.107455. Online ahead of print.ABSTRACTBoar sperm is highly susceptible to cold damage. When temperature drops to 5°C, the plasmatic membrane is destabilized. The freezing process causes a reduction of the fertility window because frozen/thawed boar sperm has less survivability. The aim of this work was to analyze the effect on sperm characteristics and response to capacitation stimuli of cooling to 5°C using a controlled protocol. Also, we evaluated if the addition of Glycerol 2% or 3% at 5°C was able to modify these parameters. For this purpose, w...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: Pablo Torres Daniela Marina Malcervelli Jonathan Hern án Cartelle Mar ía Laura Fischman Humberto Osvaldo Cisale Source Type: research
Cold-induced damage in boar sperm cooled to 5 °C in Lactose Egg Yolk (LEY) medium with different Glycerol concentrations
Anim Reprod Sci. 2024 Mar 13;264:107455. doi: 10.1016/j.anireprosci.2024.107455. Online ahead of print.ABSTRACTBoar sperm is highly susceptible to cold damage. When temperature drops to 5°C, the plasmatic membrane is destabilized. The freezing process causes a reduction of the fertility window because frozen/thawed boar sperm has less survivability. The aim of this work was to analyze the effect on sperm characteristics and response to capacitation stimuli of cooling to 5°C using a controlled protocol. Also, we evaluated if the addition of Glycerol 2% or 3% at 5°C was able to modify these parameters. For this purpose, w...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: Pablo Torres Daniela Marina Malcervelli Jonathan Hern án Cartelle Mar ía Laura Fischman Humberto Osvaldo Cisale Source Type: research
Method for evaluating milk production in mouse mammary gland
AbstractLactation is a characteristic physiological function of mammals and is important for nourishing infants and the dairy industry; however, the molecular mechanisms underlying the function remain to be elucidated. A technique to directly evaluate the quantity and quality of milk in mice is necessary for the study of the lactation mechanism in vivo. By measuring the changes in milk amount after different durations of milk accumulation (0 –24 h) using a ductal cannulation technique and oxytocin supplementation, we estimated the milk production rate at a single mammary gland level. In addition, collected milk was ava...
Source: Animal Science Journal - March 28, 2024 Category: Zoology Authors: Hibiki Sakai,
Akihiro Kamikawa Tags: TECHNICAL NOTE Source Type: research
Occurrence and characterization of Proteus mirabilis from infected farmed African catfish in Ogun State, Nigeria
CONCLUSIONS: We conclude that Proteus mirabilis colonises farmed Clarias gariepinus in Ogun State, Nigeria and the identified strain showed an evolutionary relationship with known pathogenic NCBI reference strains from other countries.PMID:38532199 | DOI:10.1007/s11033-023-08973-6 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - March 27, 2024 Category: Molecular Biology Authors: Olayinka Remilekun Anifowose Gbolahanmi Akinola Oladosu Oladipo Olufemi Omotosho Source Type: research
Inhibition of macrophage infectivity potentiator in Burkholderia pseudomallei suppresses pro-inflammatory responses in murine macrophages
DiscussionThese data highlight the potential to utilize Mip inhibitors in reducing potentially harmful pro-inflammatory responses resulting from B. pseudomallei infection in macrophages. This could be of significance since overstimulation of pro-inflammatory responses can result in immunopathology, tissue damage and septic shock. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - March 25, 2024 Category: Microbiology Source Type: research
Improved production of RNA-inhibiting antimicrobial peptide by Bacillus licheniformis MCC 2514 facilitated by a genetic algorithm optimized medium
In this study, a semi-synthetic medium was devised to overcome this challenge. Using a genetic algorithm, the production medium of AMP is optimized. The parent organism, Bacillus licheniformis MCC2514, produces AMP in very small quantities. This AMP is known to inhibit RNA biosynthesis. The findings revealed that lactose, NH4Cl and NaNO3 were crucial medium constituents for enhanced AMP synthesis. The potency of the AMP produced was studied using bacterium, Kocuria rhizophila ATCC 9341. The AMP produced from the optimized medium was eightfold higher than that produced from the unoptimized medium. Furthermore, activity was ...
Source: Bioprocess and Biosystems Engineering - March 24, 2024 Category: Biomedical Engineering Authors: Ishrat Jahan Peerzade Sarma Mutturi Prakash M Halami Source Type: research
Improved production of RNA-inhibiting antimicrobial peptide by Bacillus licheniformis MCC 2514 facilitated by a genetic algorithm optimized medium
In this study, a semi-synthetic medium was devised to overcome this challenge. Using a genetic algorithm, the production medium of AMP is optimized. The parent organism, Bacillus licheniformis MCC2514, produces AMP in very small quantities. This AMP is known to inhibit RNA biosynthesis. The findings revealed that lactose, NH4Cl and NaNO3 were crucial medium constituents for enhanced AMP synthesis. The potency of the AMP produced was studied using bacterium, Kocuria rhizophila ATCC 9341. The AMP produced from the optimized medium was eightfold higher than that produced from the unoptimized medium. Furthermore, activity was ...
Source: Bioprocess and Biosystems Engineering - March 24, 2024 Category: Biomedical Engineering Authors: Ishrat Jahan Peerzade Sarma Mutturi Prakash M Halami Source Type: research
The challenge of downstream processing of spray dried amorphous solid dispersions into minitablets designed for the paediatric population - A sustainable product development approach
The objective of the current study was to develop a formulation of spray dried indomethacin ASD suited to the production, by direct compression, of instant release paediatric minitablets. Excipients evaluated were PVP or HPMCAS in solid dispersions at the preformulation phase, and MCC and lactose as a filler in direct compression. From the studied formulations, a 3:1 ratio blend of Vivapur 200/Pharmatose 200M (MCC/lactose) with 0.5 % (w/w) magnesium stearate was found to be the most promising in tableting, and minitablets containing a 6.22 % content of spray-dried ASD of indomethacin/PVP K 29-32 could be obtained with desi...
Source: European Journal of Pharmaceutical Sciences - March 22, 2024 Category: Drugs & Pharmacology Authors: Anja Autzen Virtanen Monika My ślińska Anne Marie Healy Eoin Power Atif Madi Mia Siv én Source Type: research
The challenge of downstream processing of spray dried amorphous solid dispersions into minitablets designed for the paediatric population - A sustainable product development approach
The objective of the current study was to develop a formulation of spray dried indomethacin ASD suited to the production, by direct compression, of instant release paediatric minitablets. Excipients evaluated were PVP or HPMCAS in solid dispersions at the preformulation phase, and MCC and lactose as a filler in direct compression. From the studied formulations, a 3:1 ratio blend of Vivapur 200/Pharmatose 200M (MCC/lactose) with 0.5 % (w/w) magnesium stearate was found to be the most promising in tableting, and minitablets containing a 6.22 % content of spray-dried ASD of indomethacin/PVP K 29-32 could be obtained with desi...
Source: European Journal of Pharmaceutical Sciences - March 22, 2024 Category: Drugs & Pharmacology Authors: Anja Autzen Virtanen Monika My ślińska Anne Marie Healy Eoin Power Atif Madi Mia Siv én Source Type: research
