The beneficial effects of quinoa seed extract supplementation on ram sperm quality following cryopreservation
Anim Reprod Sci. 2024 Apr 4;264:107472. doi: 10.1016/j.anireprosci.2024.107472. Online ahead of print.ABSTRACTAlthough cryopreservation is a reliable method used in assisted reproduction to preserve genetic materials, it can stimulate the occurrence of oxidative stress, which affects sperm structure and function. This research was conducted to explore the effects of quinoa seed extracts (QSE) on ram sperm quality, oxidative biomarkers, and the gene expression of frozen-thawed ram sperm. Semen samples were diluted in extenders supplemented with 0 (QSE0), 250 (QSE1), 500 (QSE2), 750 (QSE3), and 1000 (QSE4) µg of QSE /mL, an...
Source: Animal Reproduction Science - April 10, 2024 Category: Zoology Authors: Wael A Khalil Mahmoud A E Hassan Sally Ibrahim Amer K Mohammed Mostafa A El-Harairy Sameh A Abdelnour Source Type: research
The pFSH dose affects the efficiency of in vivo embryo production in Santa In ês ewes
This study compared the follicular growth, superovulatory response, and in vivo embryo production after administering two doses of porcine follicle-stimulating hormone (pFSH) in Santa Inês ewes. The estrous cycle of 36 multiparous ewes was synchronized with the Day 0 protocol and superovulated with 133 mg (G133, n=18) or 200 mg (G200, n=18) of pFSH. Ultrasonographic evaluations of the ovaries were performed, ewes were mated and submitted to non-surgical embryo recovery. Viable blastocysts were stained with Nile Red and Hoechst. The G200 had a greater number of medium and large follicles, as well as a larger size of the th...
Source: Animal Reproduction Science - April 10, 2024 Category: Zoology Authors: Ana Clara Sarzedas Ribeiro Pedro Henrique Nicolau Pinto Juliana Dantas Rodrigues Santos Nadiala Porto Ramalho Camila Correa Roza Laeber Mario Felipe Alvarez Balaro Ribrio Ivan Tavares Pereira Batista Joanna Maria Gon çalves Souza-Fabjan Jeferson Ferreira Source Type: research
The beneficial effects of quinoa seed extract supplementation on ram sperm quality following cryopreservation
Anim Reprod Sci. 2024 Apr 4;264:107472. doi: 10.1016/j.anireprosci.2024.107472. Online ahead of print.ABSTRACTAlthough cryopreservation is a reliable method used in assisted reproduction to preserve genetic materials, it can stimulate the occurrence of oxidative stress, which affects sperm structure and function. This research was conducted to explore the effects of quinoa seed extracts (QSE) on ram sperm quality, oxidative biomarkers, and the gene expression of frozen-thawed ram sperm. Semen samples were diluted in extenders supplemented with 0 (QSE0), 250 (QSE1), 500 (QSE2), 750 (QSE3), and 1000 (QSE4) µg of QSE /mL, an...
Source: Animal Reproduction Science - April 10, 2024 Category: Zoology Authors: Wael A Khalil Mahmoud A E Hassan Sally Ibrahim Amer K Mohammed Mostafa A El-Harairy Sameh A Abdelnour Source Type: research
The pFSH dose affects the efficiency of in vivo embryo production in Santa In ês ewes
This study compared the follicular growth, superovulatory response, and in vivo embryo production after administering two doses of porcine follicle-stimulating hormone (pFSH) in Santa Inês ewes. The estrous cycle of 36 multiparous ewes was synchronized with the Day 0 protocol and superovulated with 133 mg (G133, n=18) or 200 mg (G200, n=18) of pFSH. Ultrasonographic evaluations of the ovaries were performed, ewes were mated and submitted to non-surgical embryo recovery. Viable blastocysts were stained with Nile Red and Hoechst. The G200 had a greater number of medium and large follicles, as well as a larger size of the th...
Source: Animal Reproduction Science - April 10, 2024 Category: Zoology Authors: Ana Clara Sarzedas Ribeiro Pedro Henrique Nicolau Pinto Juliana Dantas Rodrigues Santos Nadiala Porto Ramalho Camila Correa Roza Laeber Mario Felipe Alvarez Balaro Ribrio Ivan Tavares Pereira Batista Joanna Maria Gon çalves Souza-Fabjan Jeferson Ferreira Source Type: research
Pregnancy losses in Bos indicus-influenced beef and dairy recipients assigned to a fixed-time embryo transfer protocol
Anim Reprod Sci. 2024 Apr 3;264:107471. doi: 10.1016/j.anireprosci.2024.107471. Online ahead of print.ABSTRACTPregnancy losses from fixed-time embryo transfer (FTET) to calving were evaluated in Bos indicus-influenced beef and dairy recipients. Data from 4366 FTET events were collected from Nelore × Angus recipient heifers, and from 38538 FTET events in Gir × Holstein recipient heifers and cows. In beef recipients, pregnancy losses were greater (P < 0.01) from FTET (day 7 of the experiment) to day 32 compared with day 32-100 and with day 100 to calving (58.7, 39.5, and 36.7%, respectively), and did not differ (P = 0.5...
Source: Animal Reproduction Science - April 6, 2024 Category: Zoology Authors: S K Munhoz R F Cooke A K Munhoz C P Prado M H C Pereira J L M Vasconcelos Source Type: research
Pregnancy losses in Bos indicus-influenced beef and dairy recipients assigned to a fixed-time embryo transfer protocol
Anim Reprod Sci. 2024 Apr 3;264:107471. doi: 10.1016/j.anireprosci.2024.107471. Online ahead of print.ABSTRACTPregnancy losses from fixed-time embryo transfer (FTET) to calving were evaluated in Bos indicus-influenced beef and dairy recipients. Data from 4366 FTET events were collected from Nelore × Angus recipient heifers, and from 38538 FTET events in Gir × Holstein recipient heifers and cows. In beef recipients, pregnancy losses were greater (P < 0.01) from FTET (day 7 of the experiment) to day 32 compared with day 32-100 and with day 100 to calving (58.7, 39.5, and 36.7%, respectively), and did not differ (P = 0.5...
Source: Animal Reproduction Science - April 6, 2024 Category: Zoology Authors: S K Munhoz R F Cooke A K Munhoz C P Prado M H C Pereira J L M Vasconcelos Source Type: research
Pregnancy losses in Bos indicus-influenced beef and dairy recipients assigned to a fixed-time embryo transfer protocol
Anim Reprod Sci. 2024 Apr 3;264:107471. doi: 10.1016/j.anireprosci.2024.107471. Online ahead of print.ABSTRACTPregnancy losses from fixed-time embryo transfer (FTET) to calving were evaluated in Bos indicus-influenced beef and dairy recipients. Data from 4366 FTET events were collected from Nelore × Angus recipient heifers, and from 38538 FTET events in Gir × Holstein recipient heifers and cows. In beef recipients, pregnancy losses were greater (P < 0.01) from FTET (day 7 of the experiment) to day 32 compared with day 32-100 and with day 100 to calving (58.7, 39.5, and 36.7%, respectively), and did not differ (P = 0.5...
Source: Animal Reproduction Science - April 6, 2024 Category: Zoology Authors: S K Munhoz R F Cooke A K Munhoz C P Prado M H C Pereira J L M Vasconcelos Source Type: research
Pregnancy losses in Bos indicus-influenced beef and dairy recipients assigned to a fixed-time embryo transfer protocol
Anim Reprod Sci. 2024 Apr 3;264:107471. doi: 10.1016/j.anireprosci.2024.107471. Online ahead of print.ABSTRACTPregnancy losses from fixed-time embryo transfer (FTET) to calving were evaluated in Bos indicus-influenced beef and dairy recipients. Data from 4366 FTET events were collected from Nelore × Angus recipient heifers, and from 38538 FTET events in Gir × Holstein recipient heifers and cows. In beef recipients, pregnancy losses were greater (P < 0.01) from FTET (day 7 of the experiment) to day 32 compared with day 32-100 and with day 100 to calving (58.7, 39.5, and 36.7%, respectively), and did not differ (P = 0.5...
Source: Animal Reproduction Science - April 6, 2024 Category: Zoology Authors: S K Munhoz R F Cooke A K Munhoz C P Prado M H C Pereira J L M Vasconcelos Source Type: research
Analysis of blood biochemistry and non-targeted metabolomics of endometritis in dairy cows
In this study, the differential markers and metabolites of healthy cows and cows with endometritis were analyzed by measuring blood biochemical indicators and immune factors using biochemical and enzyme-linked immunosorbent assay kits combined with nontargeted metabolomics. The LC-QTOF platform was used to evaluate the serum metabolomics of healthy cows and cows with endometritis after 21-27 days of calving. The results showed that glucose, free fatty acid, calcium, sodium, albumin, and alanine aminotransferase levels were significantly lower in the serum of cows with endometritis than in healthy cows (P < 0.05). Howeve...
Source: Animal Reproduction Science - April 2, 2024 Category: Zoology Authors: Guoshang Ji Junxing Zhang Xue Feng Hui Sheng Honghong Hu Fen Li Yanfen Ma Yamei Hu Rina Na Wenfei Yang Yun Ma Source Type: research
Analysis of blood biochemistry and non-targeted metabolomics of endometritis in dairy cows
In this study, the differential markers and metabolites of healthy cows and cows with endometritis were analyzed by measuring blood biochemical indicators and immune factors using biochemical and enzyme-linked immunosorbent assay kits combined with nontargeted metabolomics. The LC-QTOF platform was used to evaluate the serum metabolomics of healthy cows and cows with endometritis after 21-27 days of calving. The results showed that glucose, free fatty acid, calcium, sodium, albumin, and alanine aminotransferase levels were significantly lower in the serum of cows with endometritis than in healthy cows (P < 0.05). Howeve...
Source: Animal Reproduction Science - April 2, 2024 Category: Zoology Authors: Guoshang Ji Junxing Zhang Xue Feng Hui Sheng Honghong Hu Fen Li Yanfen Ma Yamei Hu Rina Na Wenfei Yang Yun Ma Source Type: research
Analysis of blood biochemistry and non-targeted metabolomics of endometritis in dairy cows
In this study, the differential markers and metabolites of healthy cows and cows with endometritis were analyzed by measuring blood biochemical indicators and immune factors using biochemical and enzyme-linked immunosorbent assay kits combined with nontargeted metabolomics. The LC-QTOF platform was used to evaluate the serum metabolomics of healthy cows and cows with endometritis after 21-27 days of calving. The results showed that glucose, free fatty acid, calcium, sodium, albumin, and alanine aminotransferase levels were significantly lower in the serum of cows with endometritis than in healthy cows (P < 0.05). Howeve...
Source: Animal Reproduction Science - April 2, 2024 Category: Zoology Authors: Guoshang Ji Junxing Zhang Xue Feng Hui Sheng Honghong Hu Fen Li Yanfen Ma Yamei Hu Rina Na Wenfei Yang Yun Ma Source Type: research
Analysis of blood biochemistry and non-targeted metabolomics of endometritis in dairy cows
In this study, the differential markers and metabolites of healthy cows and cows with endometritis were analyzed by measuring blood biochemical indicators and immune factors using biochemical and enzyme-linked immunosorbent assay kits combined with nontargeted metabolomics. The LC-QTOF platform was used to evaluate the serum metabolomics of healthy cows and cows with endometritis after 21-27 days of calving. The results showed that glucose, free fatty acid, calcium, sodium, albumin, and alanine aminotransferase levels were significantly lower in the serum of cows with endometritis than in healthy cows (P < 0.05). Howeve...
Source: Animal Reproduction Science - April 2, 2024 Category: Zoology Authors: Guoshang Ji Junxing Zhang Xue Feng Hui Sheng Honghong Hu Fen Li Yanfen Ma Yamei Hu Rina Na Wenfei Yang Yun Ma Source Type: research
Factors affecting the success of laparoscopic artificial insemination in sheep
Anim Reprod Sci. 2024 Mar 14;264:107453. doi: 10.1016/j.anireprosci.2024.107453. Online ahead of print.ABSTRACTSuccessful artificial breeding underpins rapid genetic and production gains in animal agriculture. In sheep, artificial insemination with frozen semen is performed via intrauterine laparoscopy as frozen-thawed spermatozoa do not traverse the cervix in sufficient numbers for high fertility and transcervical insemination is anatomically impossible in most ewes. Historically, laparoscopic artificial insemination has always been considered reasonably successful, but recent anecdotal reports of poor fertility place it ...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: E A Spanner S P de Graaf J P Rickard Source Type: research
Development and growth of bovine calves demi-embryos
Anim Reprod Sci. 2023 Dec 21;264:107405. doi: 10.1016/j.anireprosci.2023.107405. Online ahead of print.ABSTRACTThe aim of this study was to investigate the growth and development of animals produced from demi-embryos and compare them with whole embryos from fetus to adult life. To achieve this, calves produced from fresh demi-embryos and whole embryos were individually transferred and monitored from 60 days of pregnancy until slaughter at 550 days. Ultrasound scans were conducted on fetuses at 60 and 90 days to evaluate the biparietal, abdominal, umbilical cord, orbital, and aorta diameters. Subsequently, morphological tra...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: Amada E Ynsaurralde-Rivolta Andres Rogberg-Mu ñoz Sebastian Lopez-Valiente Sebastian Maresca Alejandro Rodriguez Carlos Munar Sebastian Munilla-Leguizam ón Franco A Dellavalle Daniel Salamone Source Type: research
Cold-induced damage in boar sperm cooled to 5 °C in Lactose Egg Yolk (LEY) medium with different Glycerol concentrations
Anim Reprod Sci. 2024 Mar 13;264:107455. doi: 10.1016/j.anireprosci.2024.107455. Online ahead of print.ABSTRACTBoar sperm is highly susceptible to cold damage. When temperature drops to 5°C, the plasmatic membrane is destabilized. The freezing process causes a reduction of the fertility window because frozen/thawed boar sperm has less survivability. The aim of this work was to analyze the effect on sperm characteristics and response to capacitation stimuli of cooling to 5°C using a controlled protocol. Also, we evaluated if the addition of Glycerol 2% or 3% at 5°C was able to modify these parameters. For this purpose, w...
Source: Animal Reproduction Science - March 28, 2024 Category: Zoology Authors: Pablo Torres Daniela Marina Malcervelli Jonathan Hern án Cartelle Mar ía Laura Fischman Humberto Osvaldo Cisale Source Type: research
