Leishmanicidal activities of biosynthesized BaCO < sub > 3 < /sub > (witherite) nanoparticles and their biocompatibility with macrophages
Bioprocess Biosyst Eng. 2021 May 1. doi: 10.1007/s00449-021-02576-w. Online ahead of print.ABSTRACTThe aim of this study was cost-effective and greener synthesis of barium carbonate (BaCO3 or witherite) nanoparticles with economic importance, and to evaluate their therapeutic potentials and biocompatibility with immune cells. Barium carbonate nanoparticles were biosynthesized using black elderberry extract in one step with non-toxic precursors and simple laboratory conditions; their morphologies and specific structures were analyzed using field emission scanning electron microscopy with energy dispersive X-ray spectroscopy...
Source: Bioprocess and Biosystems Engineering - May 2, 2021 Category: Biomedical Engineering Authors: Nooshin Hashemi Hajar Q Alijani Farideh Mousazadeh Sadegh Rahi Samaneh Darijani Fatemeh Sharifi Hakimeh Khalili Siavash Iravani Fariba Borhani Niloofar Zafarnia Mehrdad Khatami Source Type: research

Leishmanicidal activities of biosynthesized BaCO < sub > 3 < /sub > (witherite) nanoparticles and their biocompatibility with macrophages
Bioprocess Biosyst Eng. 2021 May 1. doi: 10.1007/s00449-021-02576-w. Online ahead of print.ABSTRACTThe aim of this study was cost-effective and greener synthesis of barium carbonate (BaCO3 or witherite) nanoparticles with economic importance, and to evaluate their therapeutic potentials and biocompatibility with immune cells. Barium carbonate nanoparticles were biosynthesized using black elderberry extract in one step with non-toxic precursors and simple laboratory conditions; their morphologies and specific structures were analyzed using field emission scanning electron microscopy with energy dispersive X-ray spectroscopy...
Source: Bioprocess and Biosystems Engineering - May 2, 2021 Category: Biomedical Engineering Authors: Nooshin Hashemi Hajar Q Alijani Farideh Mousazadeh Sadegh Rahi Samaneh Darijani Fatemeh Sharifi Hakimeh Khalili Siavash Iravani Fariba Borhani Niloofar Zafarnia Mehrdad Khatami Source Type: research

Correction to: lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO < sub > 2 < /sub >
Bioprocess Biosyst Eng. 2021 Apr 27. doi: 10.1007/s00449-021-02575-x. Online ahead of print.NO ABSTRACTPMID:33905026 | DOI:10.1007/s00449-021-02575-x (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 27, 2021 Category: Biomedical Engineering Authors: Lizhi Hu Libin Sun Jun Li Liangjun Qi Xu Zhang Dianyu Yu Elfalleh Walid Lianzhou Jiang Source Type: research

Correction to: lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO < sub > 2 < /sub >
Bioprocess Biosyst Eng. 2021 Apr 27. doi: 10.1007/s00449-021-02575-x. Online ahead of print.NO ABSTRACTPMID:33905026 | DOI:10.1007/s00449-021-02575-x (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 27, 2021 Category: Biomedical Engineering Authors: Lizhi Hu Libin Sun Jun Li Liangjun Qi Xu Zhang Dianyu Yu Elfalleh Walid Lianzhou Jiang Source Type: research

Correction to: lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO < sub > 2 < /sub >
Bioprocess Biosyst Eng. 2021 Apr 27. doi: 10.1007/s00449-021-02575-x. Online ahead of print.NO ABSTRACTPMID:33905026 | DOI:10.1007/s00449-021-02575-x (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 27, 2021 Category: Biomedical Engineering Authors: Lizhi Hu Libin Sun Jun Li Liangjun Qi Xu Zhang Dianyu Yu Elfalleh Walid Lianzhou Jiang Source Type: research

Correction to: lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO < sub > 2 < /sub >
Bioprocess Biosyst Eng. 2021 Apr 27. doi: 10.1007/s00449-021-02575-x. Online ahead of print.NO ABSTRACTPMID:33905026 | DOI:10.1007/s00449-021-02575-x (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 27, 2021 Category: Biomedical Engineering Authors: Lizhi Hu Libin Sun Jun Li Liangjun Qi Xu Zhang Dianyu Yu Elfalleh Walid Lianzhou Jiang Source Type: research

Correction to: lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO < sub > 2 < /sub >
Bioprocess Biosyst Eng. 2021 Apr 27. doi: 10.1007/s00449-021-02575-x. Online ahead of print.NO ABSTRACTPMID:33905026 | DOI:10.1007/s00449-021-02575-x (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 27, 2021 Category: Biomedical Engineering Authors: Lizhi Hu Libin Sun Jun Li Liangjun Qi Xu Zhang Dianyu Yu Elfalleh Walid Lianzhou Jiang Source Type: research

Quantification of oxygen transfer coefficients in simulated hydrocarbon-based bioprocesses in a bubble column bioreactor
This study investigates the overall volumetric oxygen transfer coefficient (KLa) in multiphase hydrocarbon-based bioprocess under a range of hydrocarbon concentrations (HC), solid loadings (deactivated yeast) (SL) and superficial gas velocities (UG) in a bubble column reactor (BCR). KLa increased with increasing UG in the air-water system; due to an increase in the number of small bubbles which enhanced gas holdup. In air-water-yeast systems, the initial addition of yeast increased KLa significantly. Further increases in SL reduced KLa, due to increases in the bubble size with increasing SL. KLa decreased when HC was added...
Source: Bioprocess and Biosystems Engineering - April 24, 2021 Category: Biomedical Engineering Authors: Ayman A Abufalgha Robert W M Pott Kim G Clarke Source Type: research

Quantification of oxygen transfer coefficients in simulated hydrocarbon-based bioprocesses in a bubble column bioreactor
This study investigates the overall volumetric oxygen transfer coefficient (KLa) in multiphase hydrocarbon-based bioprocess under a range of hydrocarbon concentrations (HC), solid loadings (deactivated yeast) (SL) and superficial gas velocities (UG) in a bubble column reactor (BCR). KLa increased with increasing UG in the air-water system; due to an increase in the number of small bubbles which enhanced gas holdup. In air-water-yeast systems, the initial addition of yeast increased KLa significantly. Further increases in SL reduced KLa, due to increases in the bubble size with increasing SL. KLa decreased when HC was added...
Source: Bioprocess and Biosystems Engineering - April 24, 2021 Category: Biomedical Engineering Authors: Ayman A Abufalgha Robert W M Pott Kim G Clarke Source Type: research

Quantification of oxygen transfer coefficients in simulated hydrocarbon-based bioprocesses in a bubble column bioreactor
This study investigates the overall volumetric oxygen transfer coefficient (KLa) in multiphase hydrocarbon-based bioprocess under a range of hydrocarbon concentrations (HC), solid loadings (deactivated yeast) (SL) and superficial gas velocities (UG) in a bubble column reactor (BCR). KLa increased with increasing UG in the air-water system; due to an increase in the number of small bubbles which enhanced gas holdup. In air-water-yeast systems, the initial addition of yeast increased KLa significantly. Further increases in SL reduced KLa, due to increases in the bubble size with increasing SL. KLa decreased when HC was added...
Source: Bioprocess and Biosystems Engineering - April 24, 2021 Category: Biomedical Engineering Authors: Ayman A Abufalgha Robert W M Pott Kim G Clarke Source Type: research

High-efficient L-lactic acid production from inedible starchy biomass by one-step open fermentation using thermotolerant Lactobacillus rhamnosus DUT1908
Bioprocess Biosyst Eng. 2021 Apr 22. doi: 10.1007/s00449-021-02573-z. Online ahead of print.ABSTRACTThe purpose of this study was to establish a simplified operational process for lactic acid (LA) production from inedible starchy biomass by open fermentation using thermotolerant Lactobacillus rhamnosus DUT1908. One step simultaneous liquefaction, saccharification and fermentation (SLSF) was proposed to produce LA using aging paddy rice with hull (APRH) as feedstock. First, a robust microbial strain was obtained by adaptive laboratory evolution under high temperature stress. As a result, L. rhamnosus DUT1908 showed high the...
Source: Bioprocess and Biosystems Engineering - April 23, 2021 Category: Biomedical Engineering Authors: Yaqin Sun Huihui Liu Yong Yang Xu Zhou Zhilong Xiu Source Type: research

Cerium oxide nanoparticles: green synthesis using Banana peel, cytotoxic effect, UV protection and their photocatalytic activity
Bioprocess Biosyst Eng. 2021 Apr 23. doi: 10.1007/s00449-021-02569-9. Online ahead of print.ABSTRACTNanomaterials, as an active ingredient, have been widely deployed in various science and technological applications with zinc and titanium oxides nanoparticles being commonly applied in sunscreens. On similar lines, cerium oxide nanoparticles (CeO2-NPs) were synthesized using Musa sapientum peel extract, to investigate its cytotoxic effects, UV protection and photocatalytic activity. The synthesized nanoparticles were identified through Raman, Powder X-ray Diffraction (PXRD), Fourier-Transform Infrared spectroscopy (FT-IR), ...
Source: Bioprocess and Biosystems Engineering - April 23, 2021 Category: Biomedical Engineering Authors: Abdolhossein Miri Honeyeh Beiki Ahmad Najafidoust Mehrdad Khatami Mina Sarani Source Type: research

High-efficient L-lactic acid production from inedible starchy biomass by one-step open fermentation using thermotolerant Lactobacillus rhamnosus DUT1908
Bioprocess Biosyst Eng. 2021 Apr 22. doi: 10.1007/s00449-021-02573-z. Online ahead of print.ABSTRACTThe purpose of this study was to establish a simplified operational process for lactic acid (LA) production from inedible starchy biomass by open fermentation using thermotolerant Lactobacillus rhamnosus DUT1908. One step simultaneous liquefaction, saccharification and fermentation (SLSF) was proposed to produce LA using aging paddy rice with hull (APRH) as feedstock. First, a robust microbial strain was obtained by adaptive laboratory evolution under high temperature stress. As a result, L. rhamnosus DUT1908 showed high the...
Source: Bioprocess and Biosystems Engineering - April 23, 2021 Category: Biomedical Engineering Authors: Yaqin Sun Huihui Liu Yong Yang Xu Zhou Zhilong Xiu Source Type: research

Cerium oxide nanoparticles: green synthesis using Banana peel, cytotoxic effect, UV protection and their photocatalytic activity
Bioprocess Biosyst Eng. 2021 Apr 23. doi: 10.1007/s00449-021-02569-9. Online ahead of print.ABSTRACTNanomaterials, as an active ingredient, have been widely deployed in various science and technological applications with zinc and titanium oxides nanoparticles being commonly applied in sunscreens. On similar lines, cerium oxide nanoparticles (CeO2-NPs) were synthesized using Musa sapientum peel extract, to investigate its cytotoxic effects, UV protection and photocatalytic activity. The synthesized nanoparticles were identified through Raman, Powder X-ray Diffraction (PXRD), Fourier-Transform Infrared spectroscopy (FT-IR), ...
Source: Bioprocess and Biosystems Engineering - April 23, 2021 Category: Biomedical Engineering Authors: Abdolhossein Miri Honeyeh Beiki Ahmad Najafidoust Mehrdad Khatami Mina Sarani Source Type: research

Use of equivalent circuit analysis and Cole-Cole model in evaluation of bioreactor operating conditions for biomass monitoring by impedance spectroscopy
Bioprocess Biosyst Eng. 2021 Apr 21. doi: 10.1007/s00449-021-02572-0. Online ahead of print.ABSTRACTThe most important parameter in bioprocesses is biomass, where not only the quantity produced in a culture but also the behavior that is presented are important concerns. It is clear that conditions of operation in a bioreactor affect biomass production, but how operation conditions affect the measurement of biomass on-line is of special interest. We studied the effect of bioreactor operating condition variations on model parameters using impedance spectroscopy for biomass monitoring. The model parameters analyzed were capac...
Source: Bioprocess and Biosystems Engineering - April 22, 2021 Category: Biomedical Engineering Authors: Adri án Díaz Pacheco Raul Jacobo Delgado-Macuil Ángel Díaz-Pacheco Claudia Patricia Larralde-Corona Jabel Dinor ín-Téllez-Girón Victor Eric L ópez-Y-López Source Type: research

Use of equivalent circuit analysis and Cole-Cole model in evaluation of bioreactor operating conditions for biomass monitoring by impedance spectroscopy
Bioprocess Biosyst Eng. 2021 Apr 21. doi: 10.1007/s00449-021-02572-0. Online ahead of print.ABSTRACTThe most important parameter in bioprocesses is biomass, where not only the quantity produced in a culture but also the behavior that is presented are important concerns. It is clear that conditions of operation in a bioreactor affect biomass production, but how operation conditions affect the measurement of biomass on-line is of special interest. We studied the effect of bioreactor operating condition variations on model parameters using impedance spectroscopy for biomass monitoring. The model parameters analyzed were capac...
Source: Bioprocess and Biosystems Engineering - April 22, 2021 Category: Biomedical Engineering Authors: Adri án Díaz Pacheco Raul Jacobo Delgado-Macuil Ángel Díaz-Pacheco Claudia Patricia Larralde-Corona Jabel Dinor ín-Téllez-Girón Victor Eric L ópez-Y-López Source Type: research

Effects of turbulence fluctuation intensity in bioreactor of sewage treatment on physical and chemical properties of biofilms
This study was intended to provide theoretical support for the design and operation optimization of bioreactors.PMID:33871701 | DOI:10.1007/s00449-021-02566-y (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 19, 2021 Category: Biomedical Engineering Authors: Chao Luo Lisha Guo Shi Zeng Tianyu Long Source Type: research

Effects of turbulence fluctuation intensity in bioreactor of sewage treatment on physical and chemical properties of biofilms
This study was intended to provide theoretical support for the design and operation optimization of bioreactors.PMID:33871701 | DOI:10.1007/s00449-021-02566-y (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 19, 2021 Category: Biomedical Engineering Authors: Chao Luo Lisha Guo Shi Zeng Tianyu Long Source Type: research

Effects of turbulence fluctuation intensity in bioreactor of sewage treatment on physical and chemical properties of biofilms
This study was intended to provide theoretical support for the design and operation optimization of bioreactors.PMID:33871701 | DOI:10.1007/s00449-021-02566-y (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 19, 2021 Category: Biomedical Engineering Authors: Chao Luo Lisha Guo Shi Zeng Tianyu Long Source Type: research

Enhancing CO < sub > 2 < /sub > utilization by a physical absorption-based technique in microalgae culture
This study indicates that the incorporation of physical absorption enhancers could be a promising approach to overcome the problems of low CO2 utilization efficiency and high carbon source cost in algal biomass production.PMID:33864126 | DOI:10.1007/s00449-021-02570-2 (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 17, 2021 Category: Biomedical Engineering Authors: Zhong-Liang Sun Meng-Ru Xin Ping Li Li-Qin Sun Shi-Kai Wang Source Type: research

Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal
Bioprocess Biosyst Eng. 2021 Apr 17. doi: 10.1007/s00449-021-02564-0. Online ahead of print.ABSTRACTBiomass samples from a structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) were analyzed to investigate the bacterial community shift along with the changes in the C/N ratio. The C/N ratios tested were 7.6 ± 1.0 (LNC) and 2.9 ± 0.4 (HNC). The massive sequencing analyses revealed that the microbial community adjusted itself to different organic and nitrogenous applied loads, with no harm to reactor performance regarding COD and Total-N removal. Under LNC, conventional nitrificati...
Source: Bioprocess and Biosystems Engineering - April 17, 2021 Category: Biomedical Engineering Authors: Carla Elo ísa Diniz Dos Santos Rachel Biancalana Costa Camila Abreu Borges Silva Rabelo Ant ônio Djalma Nunes Ferraz Júnior Gabriela Felix Persinoti Elo ísa Pozzi Eugenio Foresti M árcia Helena Rissato Zamariolli Damianovic Source Type: research

Enhancing CO < sub > 2 < /sub > utilization by a physical absorption-based technique in microalgae culture
This study indicates that the incorporation of physical absorption enhancers could be a promising approach to overcome the problems of low CO2 utilization efficiency and high carbon source cost in algal biomass production.PMID:33864126 | DOI:10.1007/s00449-021-02570-2 (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 17, 2021 Category: Biomedical Engineering Authors: Zhong-Liang Sun Meng-Ru Xin Ping Li Li-Qin Sun Shi-Kai Wang Source Type: research

Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal
Bioprocess Biosyst Eng. 2021 Apr 17. doi: 10.1007/s00449-021-02564-0. Online ahead of print.ABSTRACTBiomass samples from a structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) were analyzed to investigate the bacterial community shift along with the changes in the C/N ratio. The C/N ratios tested were 7.6 ± 1.0 (LNC) and 2.9 ± 0.4 (HNC). The massive sequencing analyses revealed that the microbial community adjusted itself to different organic and nitrogenous applied loads, with no harm to reactor performance regarding COD and Total-N removal. Under LNC, conventional nitrificati...
Source: Bioprocess and Biosystems Engineering - April 17, 2021 Category: Biomedical Engineering Authors: Carla Elo ísa Diniz Dos Santos Rachel Biancalana Costa Camila Abreu Borges Silva Rabelo Ant ônio Djalma Nunes Ferraz Júnior Gabriela Felix Persinoti Elo ísa Pozzi Eugenio Foresti M árcia Helena Rissato Zamariolli Damianovic Source Type: research

Solid-state culture of Azospirillum brasilense: a reliable technology for biofertilizer production from laboratory to pilot scale
Bioprocess Biosyst Eng. 2021 Apr 15. doi: 10.1007/s00449-021-02537-3. Online ahead of print.ABSTRACTA biofertilizer of Azospirillum brasilense was produced in solid-state culture (SSC) from laboratory to pilot scale. Similar operation conditions (continuous aeration and mild intermittent mixing) and two dimensionless numbers with similar L/D ratio and a similar working volume were applied to reach a scale-up factor of 75. An innovative bioreactor with rotating helical ribbons (15 kg wet matter) was used at pilot scale. A mathematical model was proposed and validated to evaluate the respirometry trends at laboratory and pil...
Source: Bioprocess and Biosystems Engineering - April 16, 2021 Category: Biomedical Engineering Authors: C Mart ínez-Ramírez R Esquivel-Cote R Ferrera-Cerrato J A Mart ínez-Ruiz G Rodr íguez-Serrano G Saucedo-Casta ñeda Source Type: research

A new approach for calculating microalgae culture growth based on an inhibitory effect of the surrounding biomass
Bioprocess Biosyst Eng. 2021 Apr 16. doi: 10.1007/s00449-021-02550-6. Online ahead of print.ABSTRACTEver since the potential of algae in biotechnology was recognized, models describing the growth of algae inside photobioreactors have been proposed. These models are the basis for the optimization of process conditions and reactor designs. Over the last few decades, models became more and more elaborate with the increase of computational capacity. Thus far, these models have been based on light attenuation due to the absorption and scattering effects of the biomass. This manuscript presents a new way of predicting the appare...
Source: Bioprocess and Biosystems Engineering - April 16, 2021 Category: Biomedical Engineering Authors: Sun-Hwa Jung Christopher McHardy Cornelia Rauh Alexander Jahn Giovanni Luzi Antonio Delgado Rainer Buchholz Christoph Lindenberger Source Type: research

Solid-state culture of Azospirillum brasilense: a reliable technology for biofertilizer production from laboratory to pilot scale
Bioprocess Biosyst Eng. 2021 Apr 15. doi: 10.1007/s00449-021-02537-3. Online ahead of print.ABSTRACTA biofertilizer of Azospirillum brasilense was produced in solid-state culture (SSC) from laboratory to pilot scale. Similar operation conditions (continuous aeration and mild intermittent mixing) and two dimensionless numbers with similar L/D ratio and a similar working volume were applied to reach a scale-up factor of 75. An innovative bioreactor with rotating helical ribbons (15 kg wet matter) was used at pilot scale. A mathematical model was proposed and validated to evaluate the respirometry trends at laboratory and pil...
Source: Bioprocess and Biosystems Engineering - April 16, 2021 Category: Biomedical Engineering Authors: C Mart ínez-Ramírez R Esquivel-Cote R Ferrera-Cerrato J A Mart ínez-Ruiz G Rodr íguez-Serrano G Saucedo-Casta ñeda Source Type: research

A new approach for calculating microalgae culture growth based on an inhibitory effect of the surrounding biomass
Bioprocess Biosyst Eng. 2021 Apr 16. doi: 10.1007/s00449-021-02550-6. Online ahead of print.ABSTRACTEver since the potential of algae in biotechnology was recognized, models describing the growth of algae inside photobioreactors have been proposed. These models are the basis for the optimization of process conditions and reactor designs. Over the last few decades, models became more and more elaborate with the increase of computational capacity. Thus far, these models have been based on light attenuation due to the absorption and scattering effects of the biomass. This manuscript presents a new way of predicting the appare...
Source: Bioprocess and Biosystems Engineering - April 16, 2021 Category: Biomedical Engineering Authors: Sun-Hwa Jung Christopher McHardy Cornelia Rauh Alexander Jahn Giovanni Luzi Antonio Delgado Rainer Buchholz Christoph Lindenberger Source Type: research

Selenium nanoparticle synthesis from endangered medicinal herb (Enicostema axillare)
In conclusion, green synthesized selenium nanoparticles are less toxic and harmless. This is the first report on green synthesized selenium nanoparticles using E. axillare possessing anti-bacterial and anti-cancer activity.PMID:33855637 | DOI:10.1007/s00449-021-02565-z (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 15, 2021 Category: Biomedical Engineering Authors: Sasidharan Perumal Madhana Vigneshwari Gopal Samy Dharaneedharan Subramanian Source Type: research

Selenium nanoparticle synthesis from endangered medicinal herb (Enicostema axillare)
In conclusion, green synthesized selenium nanoparticles are less toxic and harmless. This is the first report on green synthesized selenium nanoparticles using E. axillare possessing anti-bacterial and anti-cancer activity.PMID:33855637 | DOI:10.1007/s00449-021-02565-z (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - April 15, 2021 Category: Biomedical Engineering Authors: Sasidharan Perumal Madhana Vigneshwari Gopal Samy Dharaneedharan Subramanian Source Type: research

Exploring the potential of photosynthetic induction factor for the commercial production of fucoxanthin in Phaeodactylum tricornutum
In this study, we demonstrated the feasibility of increasing fucoxanthin bioaccumulation in P. tricornutum by promoting photosynthetic activity of this diatom. Specifically, this study investigated the effects of different concentrations of the photosynthetic induction factor (PIF) on fucoxanthin content and biosynthesis, on chlorophyll fluorescence characteristics, and on the expression of photosynthesis-related genes in P. tricornutum. The results showed that the optimal PIF concentration was 1 µg L-1, while optimal time was 48 h, with the effect decreasing at 72 h. Fucoxanthin content increased by 44.2% compared t...
Source: Bioprocess and Biosystems Engineering - April 12, 2021 Category: Biomedical Engineering Authors: Shenrui Li Xiaoyun Zheng Qingshu Fang Yifu Gong Heyu Wang Source Type: research

Exploring the potential of photosynthetic induction factor for the commercial production of fucoxanthin in Phaeodactylum tricornutum
In this study, we demonstrated the feasibility of increasing fucoxanthin bioaccumulation in P. tricornutum by promoting photosynthetic activity of this diatom. Specifically, this study investigated the effects of different concentrations of the photosynthetic induction factor (PIF) on fucoxanthin content and biosynthesis, on chlorophyll fluorescence characteristics, and on the expression of photosynthesis-related genes in P. tricornutum. The results showed that the optimal PIF concentration was 1 µg L-1, while optimal time was 48 h, with the effect decreasing at 72 h. Fucoxanthin content increased by 44.2% compared t...
Source: Bioprocess and Biosystems Engineering - April 12, 2021 Category: Biomedical Engineering Authors: Shenrui Li Xiaoyun Zheng Qingshu Fang Yifu Gong Heyu Wang Source Type: research

Exploring the potential of photosynthetic induction factor for the commercial production of fucoxanthin in Phaeodactylum tricornutum
In this study, we demonstrated the feasibility of increasing fucoxanthin bioaccumulation in P. tricornutum by promoting photosynthetic activity of this diatom. Specifically, this study investigated the effects of different concentrations of the photosynthetic induction factor (PIF) on fucoxanthin content and biosynthesis, on chlorophyll fluorescence characteristics, and on the expression of photosynthesis-related genes in P. tricornutum. The results showed that the optimal PIF concentration was 1 µg L-1, while optimal time was 48 h, with the effect decreasing at 72 h. Fucoxanthin content increased by 44.2% compared t...
Source: Bioprocess and Biosystems Engineering - April 12, 2021 Category: Biomedical Engineering Authors: Shenrui Li Xiaoyun Zheng Qingshu Fang Yifu Gong Heyu Wang Source Type: research

Transporter proteins in Zymomonas mobilis contribute to the tolerance of lignocellulose-derived phenolic aldehyde inhibitors
Bioprocess Biosyst Eng. 2021 Apr 10. doi: 10.1007/s00449-021-02567-x. Online ahead of print.ABSTRACTTransporter proteins are of great importance for improving the tolerance of fermentation strains to lignocellulose-derived furans and phenolic inhibitors. Different from the documented transporter proteins responsible for the tolerance of furfural and 5-hydroxymethyl-furfural (HMF), transporters responsible for that of varied phenolic aldehyde inhibitors were less investigated and elucidated. Here, an interesting phenomenon was found that no phenolic alcohols were accumulated from phenolic aldehydes degradation in Zymomonas ...
Source: Bioprocess and Biosystems Engineering - April 11, 2021 Category: Biomedical Engineering Authors: Xia Yi Ling Lin Jun Mei Wei Wang Source Type: research

Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor
Bioprocess Biosyst Eng. 2021 Apr 8. doi: 10.1007/s00449-021-02560-4. Online ahead of print.ABSTRACTTo improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn...
Source: Bioprocess and Biosystems Engineering - April 8, 2021 Category: Biomedical Engineering Authors: Li-Qun Jin Xian-Xiao Chen Yi-Ting Jin Jun-Kang Shentu Zhi-Qiang Liu Yu-Guo Zheng Source Type: research

Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor
Bioprocess Biosyst Eng. 2021 Apr 8. doi: 10.1007/s00449-021-02560-4. Online ahead of print.ABSTRACTTo improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn...
Source: Bioprocess and Biosystems Engineering - April 8, 2021 Category: Biomedical Engineering Authors: Li-Qun Jin Xian-Xiao Chen Yi-Ting Jin Jun-Kang Shentu Zhi-Qiang Liu Yu-Guo Zheng Source Type: research

Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor
Bioprocess Biosyst Eng. 2021 Apr 8. doi: 10.1007/s00449-021-02560-4. Online ahead of print.ABSTRACTTo improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn...
Source: Bioprocess and Biosystems Engineering - April 8, 2021 Category: Biomedical Engineering Authors: Li-Qun Jin Xian-Xiao Chen Yi-Ting Jin Jun-Kang Shentu Zhi-Qiang Liu Yu-Guo Zheng Source Type: research

Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor
Bioprocess Biosyst Eng. 2021 Apr 8. doi: 10.1007/s00449-021-02560-4. Online ahead of print.ABSTRACTTo improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn...
Source: Bioprocess and Biosystems Engineering - April 8, 2021 Category: Biomedical Engineering Authors: Li-Qun Jin Xian-Xiao Chen Yi-Ting Jin Jun-Kang Shentu Zhi-Qiang Liu Yu-Guo Zheng Source Type: research

Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus
Bioprocess Biosyst Eng. 2021 Apr 5. doi: 10.1007/s00449-021-02555-1. Online ahead of print.ABSTRACTThe production of biocompounds through the cultivation of filamentous microorganisms is mainly affected by Oxygen Transfer Rate (OTR) and shear rate ([Formula: see text]) conditions. Despite efforts have been made to evaluate the effect of operating variables (impeller speed, N; and airflow rate, ϕair) on clavulanic acid production, no analysis regarding the effect of OTR and [Formula: see text] was made. Then, the aim of this study was to evaluate the dissociated effect of physical phenomena such as oxygen transfer and shea...
Source: Bioprocess and Biosystems Engineering - April 6, 2021 Category: Biomedical Engineering Authors: Renata M M G P Ribeiro Mateus N Esperan ça Ana P A Sousa Álvaro Baptista Neto Marcel O Cerri Source Type: research

Dual effects of hypoxia on proliferation and osteogenic differentiation of mouse clonal mesenchymal stem cells
Bioprocess Biosyst Eng. 2021 Apr 5. doi: 10.1007/s00449-021-02563-1. Online ahead of print.ABSTRACTMouse clonal mesenchymal stem cells (mc-MSCs) were cultured on a Cytodex 3 microcarrier in a spinner flask for a suspension culture under hypoxia condition to increase mass productivity. The hypoxia environment was established using 4.0 mM Na2SO3 with 10 μM or 100 µM CoCl2 for 24 h in a low glucose DMEM medium. As a result, the proliferation of mc-MSCs under hypoxic conditions was 1.56 times faster than the control group over 7 days. The gene expression of HIF-1a and VEGFA increased 4.62 fold and 2.07 fold, respectiv...
Source: Bioprocess and Biosystems Engineering - April 6, 2021 Category: Biomedical Engineering Authors: Hyoungki Kim Soonjo Kwon Source Type: research

Green synthesis of silver nanoparticles using Annona squamosa L. seed extract: characterization, photocatalytic and biological activity assay
Bioprocess Biosyst Eng. 2021 Apr 6. doi: 10.1007/s00449-021-02562-2. Online ahead of print.ABSTRACTThe aqueous seed extract of Annona squamosa L. was used as a reducing and stabilizing agent for the synthesis of silver nanoparticles (AgNPs). The formation of AgNPs in aqueous silver nitrate solution after the addition of the extract was indicated by a colour change from pale yellow to dark brown corresponding to a λmax at 430 nm. The phytochemicals in the extract, responsible for efficient capping and stabilization of the nanoparticles, were identified by FTIR. Powder XRD pattern demonstrated the polycrystalline natu...
Source: Bioprocess and Biosystems Engineering - April 6, 2021 Category: Biomedical Engineering Authors: Vimala Jose Lidiya Raphel K S Aiswariya Paulson Mathew Source Type: research

Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus
Bioprocess Biosyst Eng. 2021 Apr 5. doi: 10.1007/s00449-021-02555-1. Online ahead of print.ABSTRACTThe production of biocompounds through the cultivation of filamentous microorganisms is mainly affected by Oxygen Transfer Rate (OTR) and shear rate ([Formula: see text]) conditions. Despite efforts have been made to evaluate the effect of operating variables (impeller speed, N; and airflow rate, ϕair) on clavulanic acid production, no analysis regarding the effect of OTR and [Formula: see text] was made. Then, the aim of this study was to evaluate the dissociated effect of physical phenomena such as oxygen transfer and shea...
Source: Bioprocess and Biosystems Engineering - April 6, 2021 Category: Biomedical Engineering Authors: Renata M M G P Ribeiro Mateus N Esperan ça Ana P A Sousa Álvaro Baptista Neto Marcel O Cerri Source Type: research

Dual effects of hypoxia on proliferation and osteogenic differentiation of mouse clonal mesenchymal stem cells
Bioprocess Biosyst Eng. 2021 Apr 5. doi: 10.1007/s00449-021-02563-1. Online ahead of print.ABSTRACTMouse clonal mesenchymal stem cells (mc-MSCs) were cultured on a Cytodex 3 microcarrier in a spinner flask for a suspension culture under hypoxia condition to increase mass productivity. The hypoxia environment was established using 4.0 mM Na2SO3 with 10 μM or 100 µM CoCl2 for 24 h in a low glucose DMEM medium. As a result, the proliferation of mc-MSCs under hypoxic conditions was 1.56 times faster than the control group over 7 days. The gene expression of HIF-1a and VEGFA increased 4.62 fold and 2.07 fold, respectiv...
Source: Bioprocess and Biosystems Engineering - April 6, 2021 Category: Biomedical Engineering Authors: Hyoungki Kim Soonjo Kwon Source Type: research

Green synthesis of silver nanoparticles using Annona squamosa L. seed extract: characterization, photocatalytic and biological activity assay
Bioprocess Biosyst Eng. 2021 Apr 6. doi: 10.1007/s00449-021-02562-2. Online ahead of print.ABSTRACTThe aqueous seed extract of Annona squamosa L. was used as a reducing and stabilizing agent for the synthesis of silver nanoparticles (AgNPs). The formation of AgNPs in aqueous silver nitrate solution after the addition of the extract was indicated by a colour change from pale yellow to dark brown corresponding to a λmax at 430 nm. The phytochemicals in the extract, responsible for efficient capping and stabilization of the nanoparticles, were identified by FTIR. Powder XRD pattern demonstrated the polycrystalline natu...
Source: Bioprocess and Biosystems Engineering - April 6, 2021 Category: Biomedical Engineering Authors: Vimala Jose Lidiya Raphel K S Aiswariya Paulson Mathew Source Type: research

Biomediated  synthesis, characterization, and biological applications of nickel oxide nanoparticles derived from Toona ciliata, Ficus carica and Pinus roxburghii
Bioprocess Biosyst Eng. 2021 Apr 5. doi: 10.1007/s00449-021-02528-4. Online ahead of print.ABSTRACTBiomediated ecofriendly method for the synthesis of nickel oxide nanoparticles using plants extracts (Toona ciliata, Ficus carica and Pinus roxburghii) has been reported. The nanoparticles so obtained were characterized by various techniques such as ultraviolet-visible, powder X-ray diffraction, Fourier transform infrared spectroscopy, attenuated total reflectance spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, thermogravimetric analysis and fluorescence spec...
Source: Bioprocess and Biosystems Engineering - April 5, 2021 Category: Biomedical Engineering Authors: Azar Ullah Mirza Mohd Shoeb Khan Abdul Kareem Shahab A A Nami Shahnawaz Ahmad Bhat Abdulrahman Mohammad Paramjit Singh Nahid Nishat Source Type: research

Biomediated  synthesis, characterization, and biological applications of nickel oxide nanoparticles derived from Toona ciliata, Ficus carica and Pinus roxburghii
Bioprocess Biosyst Eng. 2021 Apr 5. doi: 10.1007/s00449-021-02528-4. Online ahead of print.ABSTRACTBiomediated ecofriendly method for the synthesis of nickel oxide nanoparticles using plants extracts (Toona ciliata, Ficus carica and Pinus roxburghii) has been reported. The nanoparticles so obtained were characterized by various techniques such as ultraviolet-visible, powder X-ray diffraction, Fourier transform infrared spectroscopy, attenuated total reflectance spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, thermogravimetric analysis and fluorescence spec...
Source: Bioprocess and Biosystems Engineering - April 5, 2021 Category: Biomedical Engineering Authors: Azar Ullah Mirza Mohd Shoeb Khan Abdul Kareem Shahab A A Nami Shahnawaz Ahmad Bhat Abdulrahman Mohammad Paramjit Singh Nahid Nishat Source Type: research

Effect of confinement of horse heart cytochrome c and formate dehydrogenase from Candida boidinii on mesoporous carbons on their catalytic activity
This study reports the immobilization of two biocatalysts (e.g., cytochrome c-Cyt c-and the non-metalloenzyme formate dehydrogenase from Candida boidinii-cbFDH) on a series of mesoporous carbons with controlled pore sizes. The catalytic activity of the nanoconfined proteins was correlated with the pore size distribution of the carbon materials used as supports. The electrochemical behaviour of nanoconfined Cyt c showed direct electron transfer electroactivity in pore sizes matching tightly the protein dimension. The pseudo-peroxidase activity towards H2O2 reduction was enhanced at pH 4.0, due to the protein conformational ...
Source: Bioprocess and Biosystems Engineering - April 4, 2021 Category: Biomedical Engineering Authors: Naiara Hern ández-Ibáñez Vicente Montiel Alicia Gomis-Berenguer Conchi Ania Jes ús Iniesta Source Type: research

Performances of simultaneous enhanced removal of nitrogen and phosphorus via biological aerated filter with biochar as fillers under low dissolved oxygen for digested swine wastewater treatment
This study aims to explore the feasibility of biochar as a carrier to improve the simultaneous removal of nitrogen and phosphorus in biological aerated filters (BAFs) for treating low C/N digested swine wastewater (DSW). Two similar BAFs (BAF-A with hydrophobic polypropylene resin as fillers and BAF-B with bamboo biochar as carrier) were developed for DSW treatment. Results showed that the NH4+-N, TN, and TP removal performances in BAF-B were higher than those in BAF-A. Carrier type had an obvious influence on the structures and diversity of the microbial population. The biochar carrier in BAF-B was conducive to the enrich...
Source: Bioprocess and Biosystems Engineering - April 1, 2021 Category: Biomedical Engineering Authors: Xin Xin Siqiang Liu Jiawei Qin Zhixiang Ye Wei Liu Shuhong Fang Jie Yang Source Type: research

Performances of simultaneous enhanced removal of nitrogen and phosphorus via biological aerated filter with biochar as fillers under low dissolved oxygen for digested swine wastewater treatment
This study aims to explore the feasibility of biochar as a carrier to improve the simultaneous removal of nitrogen and phosphorus in biological aerated filters (BAFs) for treating low C/N digested swine wastewater (DSW). Two similar BAFs (BAF-A with hydrophobic polypropylene resin as fillers and BAF-B with bamboo biochar as carrier) were developed for DSW treatment. Results showed that the NH4+-N, TN, and TP removal performances in BAF-B were higher than those in BAF-A. Carrier type had an obvious influence on the structures and diversity of the microbial population. The biochar carrier in BAF-B was conducive to the enrich...
Source: Bioprocess and Biosystems Engineering - April 1, 2021 Category: Biomedical Engineering Authors: Xin Xin Siqiang Liu Jiawei Qin Zhixiang Ye Wei Liu Shuhong Fang Jie Yang Source Type: research

Performances of simultaneous enhanced removal of nitrogen and phosphorus via biological aerated filter with biochar as fillers under low dissolved oxygen for digested swine wastewater treatment
This study aims to explore the feasibility of biochar as a carrier to improve the simultaneous removal of nitrogen and phosphorus in biological aerated filters (BAFs) for treating low C/N digested swine wastewater (DSW). Two similar BAFs (BAF-A with hydrophobic polypropylene resin as fillers and BAF-B with bamboo biochar as carrier) were developed for DSW treatment. Results showed that the NH4+-N, TN, and TP removal performances in BAF-B were higher than those in BAF-A. Carrier type had an obvious influence on the structures and diversity of the microbial population. The biochar carrier in BAF-B was conducive to the enrich...
Source: Bioprocess and Biosystems Engineering - April 1, 2021 Category: Biomedical Engineering Authors: Xin Xin Siqiang Liu Jiawei Qin Zhixiang Ye Wei Liu Shuhong Fang Jie Yang Source Type: research

Dynamic content changes of cordycepin and adenosine and transcriptome in Cordyceps kyushuensis Kob at different fermentation stages
Bioprocess Biosyst Eng. 2021 Mar 30. doi: 10.1007/s00449-021-02561-3. Online ahead of print.ABSTRACT20% (w/w) Astragali radix was added to the rice medium to cultivate C. kyushuensis Kob. The fermentation product was collected at mycelium stage, coloring stage, stromata-forming initial stage and fruiting body stage of C. kyushuensis Kob. The dynamic content changes of cordycepin and adenosine were detected at different fermentation stages. In the rice medium with Astragalus radix, both cordycepin and adenosine reached the highest content value on the 30th day of fermentation, 17.31 mg/g and 0.94 mg/g, respectively, which w...
Source: Bioprocess and Biosystems Engineering - March 31, 2021 Category: Biomedical Engineering Authors: Junyu Zhang Tongtong Jian Yu Zhang Guoying Zhang Jianya Ling Source Type: research