Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols.
This study reports the properties of immobilized MAS1-H108A lipase from marine Streptomyces sp. strain W007 on XAD1180 resin and its application in the synthesis of n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) for the first time. It was found that the optimal temperature and pH for both immobilized MAS1-H108A lipase and free lipase MAS1-H108A were 70 °C and 7.0, respectively. However, immobilized MAS1-H108A lipase exhibited higher thermostability when compared with free lipase MAS1-H108A. It was also interesting that both immobilized MAS1-H108A lipase and free lipase MAS1-H108A showed no regi...
Source: Bioprocess and Biosystems Engineering - November 20, 2020 Category: Biomedical Engineering Authors: Wang X, Zhao X, Qin X, Zhao Z, Yang B, Wang Y Tags: Bioprocess Biosyst Eng Source Type: research

Bacterial cellulose as an oleaginous yeast cell carrier for soybean oil refinery effluent treatment and pyrolysis oil production.
In this study, it was applied to immobilization of oleaginous yeasts for treating soybean oil refinery effluent. The immobilization percentage reached 50%, and the removal of chemical oxygen demand and oil content reached 92.1% and 93.1%, respectively, during dynamic immobilization using a mass percentage of bacterial cellulose of 30% and an immobilization time of 24 h, which were significantly higher than those of free oleaginous yeasts or yeasts immobilized by bacterial cellulose from rich medium. The immobilized oleaginous yeasts facilitated the recovery of the yeasts and effectively treated three batches of soybea...
Source: Bioprocess and Biosystems Engineering - November 19, 2020 Category: Biomedical Engineering Authors: Qiao N, Fan X, Hu S, Zhang X, Wang L, Du Y, Wang L, Zhang X, Yu D Tags: Bioprocess Biosyst Eng Source Type: research

High-level expression of a β-mannanase (manB) in Pichia pastoris GS115 for mannose production with Penicillium brevicompactum fermentation pretreatment of soybean meal.
High-level expression of a β-mannanase (manB) in Pichia pastoris GS115 for mannose production with Penicillium brevicompactum fermentation pretreatment of soybean meal. Bioprocess Biosyst Eng. 2020 Nov 16;: Authors: Chen M, Wang J, Lin L, Wei W, Shen Y, Wei D Abstract An endo-1,4-β-mannanase gene (manB) from a Bacillus pumilus Nsic-2 grown in a stinky tofu emulsion was cloned and expressed in Pichia pastoris GS115. After characterized, the endo-1,4-β-mannanase (manB) show maximum activity at pH 6.0 and 50 °C with LBG as substrate and perform high stability at a range of pH 6-8....
Source: Bioprocess and Biosystems Engineering - November 16, 2020 Category: Biomedical Engineering Authors: Chen M, Wang J, Lin L, Wei W, Shen Y, Wei D Tags: Bioprocess Biosyst Eng Source Type: research

Effect of baffle structure on flow field characteristics of orbitally shaken bioreactor.
Abstract Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were simulated, and the turbulence, dissolved oxygen and shear strain rate of the bioreactor were analyzed. The results showed that the quasi-steady-state flow patterns of the unbaffled shaking bioreactors were broken for the bioreactors with the strengthening effects of baffles. The mixing and the oxygen volumetri...
Source: Bioprocess and Biosystems Engineering - November 16, 2020 Category: Biomedical Engineering Authors: Lu Z, Li C, Fei L, Zhang H, Pan Y Tags: Bioprocess Biosyst Eng Source Type: research

Effect of the tetracycline antibiotics on performance and microbial community of microbial fuel cell.
Abstract The adverse effect of tetracycline antibiotics on microbial activity is one of the serious risks for the biologic wastewater treatment process. The microbial fuel cells (MFCs) are a promising technology for wastewater treatment and renewable power generation process. For this reason, the investigation of the inhibition effect of the tetracyclines on the MFCs is essential for reducing damage on the environment. This paper focused on the performance of MFCs under different antibiotic concentrations at the range of 0.25-50 mg/L. The power generation performance, microbial community and biofilm character...
Source: Bioprocess and Biosystems Engineering - November 12, 2020 Category: Biomedical Engineering Authors: Topcu Ş, Taşkan E Tags: Bioprocess Biosyst Eng Source Type: research

Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation.
This study established the in vitro non-GMO root culture of MA and optimized the root density, precursor feeding, and elicitors for stilbene productivity. A root culture with optimal inoculum density (3 g/flask of 30 mL medium) accumulated mulberroside A, oxyresveratrol, and resveratrol at 18.7 ± 1.00 mg/g, 136 ± 5.05 µg/g, and 41.6 ± 5.84 µg/g dry weight (DW), respectively. The feeding of L-tyrosine shortened the time required to reach the stilbene productive stage. Root cultures co-treated with 200 µM methyl jas...
Source: Bioprocess and Biosystems Engineering - November 10, 2020 Category: Biomedical Engineering Authors: Inyai C, Yusakul G, Komaikul J, Kitisripanya T, Likhitwitayawuid K, Sritularak B, Putalun W Tags: Bioprocess Biosyst Eng Source Type: research

Broad-spectrum electrochemical immunosensor based on one-step electrodeposition of AuNP-Abs and Prussian blue nanocomposite for organophosphorus pesticide detection.
In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs to form a gold-labeled probe (AuNPs-Abs), which enhances the effective binding of antibodies to nanomaterials. Prussian blue (PB) was added to electrodeposition solution to enhance the conductivity, resulting in superior electrochemical performance. The AuNP-Abs-PB composite film was prepared by electrodeposition on the electrode surface to improve the anti-interference abi...
Source: Bioprocess and Biosystems Engineering - November 8, 2020 Category: Biomedical Engineering Authors: Dong H, Zhao Q, Li J, Xiang Y, Liu H, Guo Y, Yang Q, Sun X Tags: Bioprocess Biosyst Eng Source Type: research

Transcriptome-metabolome analysis of fatty acid of Bamei pork and Gansu Black pork in China.
In this study, Bamei pigs breeding in Huzhu farm of QingHai province were selected as the test object, compared with Gansu Black pigs. Four indexes of nutmeg acid (DX1), palmitic acid (DX2), stearic acid (DX3) and linoleic acid (DX4) were set. The expression profiles of fat metabolism related genes between the two groups samples were analysed by GCMS metabolomics and transcriptomics, then coexpression network analysis were conducted to obtain phenotypic related genes. The results showed that the metabolic levels of DX3 and DX4 were significantly higher than those of other fatty acids. Among these differences, the ENSSSCG00...
Source: Bioprocess and Biosystems Engineering - November 7, 2020 Category: Biomedical Engineering Authors: Xi B, Luo J, Gao YQ, Yang XL, Guo TF, Li WH, Du TQ Tags: Bioprocess Biosyst Eng Source Type: research

Two-step enzymatic synthesis of α-linolenic acid-enriched diacylglycerols with high purities from silkworm pupae oil.
In this study, α-linolenic acid-enriched diacylglycerols (ALA-DAGs) were prepared via a two-step enzymatic way by combi-lipase using silkworm pupae oils as substrates. Firstly, several factors including temperature, mass ratio of water to oil, pH and enzyme loading were optimized for the hydrolysis of silkworm pupae oil. The maximum fatty acid content (96.51%) was obtained under the conditions: temperature 40 °C, water/oil 3:2 (w/w), pH 7, lipase TL100L loading 400 U/g, lipase PCL loading 30 U/g. Then, ALA was enriched by urea inclusion, with an increased ALA content of 82.50% being obtained. Secon...
Source: Bioprocess and Biosystems Engineering - November 7, 2020 Category: Biomedical Engineering Authors: Liu X, Shi W, Xu L, Yang B, Liao S, Lan D, Wang W, Wang Y Tags: Bioprocess Biosyst Eng Source Type: research

Status of the application of exogenous enzyme technology for the development of natural plant resources.
Abstract Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich source of enzymes. Amylase, cellulase, protease, pectinase, glycosidase, tannase, and polyphenol oxidase are among the most widely used such enzymes. Currently, the application of exogenous enzyme technology in the development of natural plant resources mainly focuses on improving the taste and fl...
Source: Bioprocess and Biosystems Engineering - November 4, 2020 Category: Biomedical Engineering Authors: Yuan B, Zhou S, Liu C, Zhang S, Li J, Liu A Tags: Bioprocess Biosyst Eng Source Type: research

CeO2 foam-like nanostructure: biosynthesis and their efficient removal of hazardous dye.
In this study, CeO2 (cerium oxide) nanoparticles were synthesized using Pinus halepensis pollen and were characterized by field emission scanning electron microscopy (FESEM), powder X-ray diffraction (PXRD) and Raman spectroscopy. The results showed that the ensuing CeO2 nanostructures, ranging in size from 5 to 25 nm, had high porosity. Synthesized CeO2 showed the effective catalytic activity towards the photocatalytic removal of dyes. In this work, the photocatalytic activity to removal dye (methyl violet 2B), in the absence of UV radiation, using cerium dioxide nanoparticles (CeO2-NP) was determined. In this resear...
Source: Bioprocess and Biosystems Engineering - November 2, 2020 Category: Biomedical Engineering Authors: Alinaghi Langari A, Soltaninezhad S, Zafarnia N, Heidari M, Varma RS, Ebrahimi Z, Azhdari S, Borhani F, Khatami M Tags: Bioprocess Biosyst Eng Source Type: research

Development of new media formulations for cell culture operations based on regression models.
Abstract The paper discusses modelling and optimization of multi-component cell culture medium. The specific productivity (Qp) was considered a function of the medium components and possible interactions described by linear factors, two-way interactions and squared terms that results in a high dimensional problem where the number of variables p (represented by the medium components and their interactions) is much larger than the number of observations n. Principal Components Regression (PCR), Partial Least Squares (PLS), Lasso and Elastic Net regressions were compared as modelling tools to deal with a high dimensi...
Source: Bioprocess and Biosystems Engineering - October 28, 2020 Category: Biomedical Engineering Authors: Carvalho M, Riesberg J, Budman H Tags: Bioprocess Biosyst Eng Source Type: research

Process strategies to improve biocatalytic depolymerization of post-consumer PET packages in bioreactors, and investigation on consumables cost reduction.
In this study, investigation of pH control and fractionation of enzyme feeding were explored in post-consumed PET (PC-PET) hydrolysis reactions catalyzed by Humicola insolens cutinase (HiC) in stirred reactors. It was found that the unbuffered reaction provided of pH control by 0.5 M NaOH addition showed 2.39-fold improvement in the released monomers (to a total of 26.3 mM), comparatively to the Tris-HCl-buffered reaction. In addition, it was observed a possibility of reducing the enzyme loading used in the process by half, leading to an increase of 2.41-fold in the specific terephthalic acid concentration releas...
Source: Bioprocess and Biosystems Engineering - October 28, 2020 Category: Biomedical Engineering Authors: Carniel A, Gomes ADC, Coelho MAZ, de Castro AM Tags: Bioprocess Biosyst Eng Source Type: research

"One-step" characterization platform for pathogenic genetics of Staphylococcus aureus.
This study realized the one-step detection of main toxin factors in S. aureus. PMID: 33112989 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - October 28, 2020 Category: Biomedical Engineering Authors: Li Y, Qiu Y, Ye C, Chen L, Liang Y, Huang TY, Zhang L, Liu J Tags: Bioprocess Biosyst Eng Source Type: research

Experimental investigation of the adsorption and desorption of cellulase enzymes on zeolite- β for enzyme recycling applications.
Experimental investigation of the adsorption and desorption of cellulase enzymes on zeolite-β for enzyme recycling applications. Bioprocess Biosyst Eng. 2020 Oct 16;: Authors: Jampana SR, Jia L, Ramarao BV, Kumar D Abstract The recyclability of cellulase enzymes using zeolite and polyethylene glycol (PEG) was investigated. The cellulase enzymes from cellulose hydrolysate suspensions were adsorbed onto zeolite-β under typical working conditions (pH 5). PEG having a molecular weight of 200 Da and 20 kDa was used as an eluent to desorb the cellulase enzymes from zeolite-β. Adsorp...
Source: Bioprocess and Biosystems Engineering - October 16, 2020 Category: Biomedical Engineering Authors: Jampana SR, Jia L, Ramarao BV, Kumar D Tags: Bioprocess Biosyst Eng Source Type: research

Expression of porcine interferon- α and its bioactivity analysis in vitro and in vivo.
In this study, the amino acid sequence of porcine interferon α (PoIFN-α) was analyzed. Seven substitutions, S38F, H40Q, F43L, N78D, Y86C, S151A, and R156T, were mutated and obtained by aligning the sequences of PoIFN-α subtypes. The PoIFN-α mutants were designed, expressed, and purified in E. coli. The antiviral activities of these PoIFN-αs were measured in Vero and swine testis cells against vesicular stomatitis virus (VSV). Their inhibitory abilities on pseudorabies virus (PRV) were also examined. Commercial PoIFN-α was used as a control. We found the ideal inducer concentration of iso...
Source: Bioprocess and Biosystems Engineering - October 13, 2020 Category: Biomedical Engineering Authors: Wang P, Xia L, Liang X, Han F, Ren H, Zhang Y, Wei Z Tags: Bioprocess Biosyst Eng Source Type: research

Amino modified magnetic halloysite nanotube supporting chloroperoxidase immobilization: enhanced stability, reusability, and efficient degradation of pesticide residue in wastewater.
Abstract Halloysite nanotube (HNT) is a natural bio-compatible and stable nanomaterial available in abundance at low-cost. In this work, HNT was modified by two strategies to make it suitable for supporting immobilization of chloroperoxidase (CPO). Firstly, Fe3O4 nanoparticles were deposited on HNT, so magnetic separation can be used instead of centrifugation. Then, the magnetic HNT was modified by 3-aminopropyltriethoxysilane (APTES), which can provide amine group on surface of HNT and meanwhile inhibit the agglomeration of magnetic HNT. Then, HNT-Fe3O4 -APTES was linked with branched polyethyleneimine (PEI) to p...
Source: Bioprocess and Biosystems Engineering - October 12, 2020 Category: Biomedical Engineering Authors: Zhu X, Fan X, Wang Y, Zhai Q, Hu M, Li S, Jiang Y Tags: Bioprocess Biosyst Eng Source Type: research

Biogenic preparation and characterization of Pyropia yezoensis silver nanoparticles (P.y AgNPs) and their antibacterial activity against Pseudomonas aeruginosa.
This study features biogenic synthesis of silver nanoparticles using an aqueous extract of the marine red algae Pyropia yezoensis. The formation of silver nanoparticles was initially confirmed by UV-Vis spectroscopy and FTIR spectra were used to identify functional groups. The average crystalline size of the silver nanoparticles was around 20-22 nm, as determined by XRD analysis. Particle size was confirmed by SEM and TEM analyses, which also showed spherical particles without agglomeration. The antibacterial properties of the nanoparticles were assessed against both Gram-positive and Gram-negative bacterial cultures ...
Source: Bioprocess and Biosystems Engineering - October 11, 2020 Category: Biomedical Engineering Authors: Ulagesan S, Nam TJ, Choi YH Tags: Bioprocess Biosyst Eng Source Type: research

Biological assisted organic sulfur removal from low rank indigenous coal using airlift bioreactor.
Abstract Combustion of coal create many harmful gases which effect on human health as well as on environment. The sulfur in coal limits its own use, and bio-desulfurization (BDS) shows enormous development potential and the prospects for the application of coal desulfurization. Present study highlights the bioprocess strategies for reduction of sulfur content from coal before combustion. The bioprocess involved the use of Airlift Bioreactor along with Rhodococcus sp. ATCC55309 as biocatalyst. Different nutritional and operational parameters involved to promote sulfur reduction at maximum level. The parameters were...
Source: Bioprocess and Biosystems Engineering - October 9, 2020 Category: Biomedical Engineering Authors: Jatoi AS, Aziz S, Soomro SA Tags: Bioprocess Biosyst Eng Source Type: research

Environmental pollution reducing strategy for scouring of undegummed sisal fibers using xylanase and pectinase enzymes.
This study was undertaken to investigate the potential of bioscouring in the processing of undegummed sisal fibers, using xylano-pectinolytic enzymes. Optimum bioscouring was obtained at pH 8.5 and 50 mM buffer molarity, using xylanase (10 IU) and pectinase (8 IU), with a material to liquor proportion of 1:25 (g:ml), EDTA (2 mM) and Tween 80 (0.5%), at 50 °C temperature with agitation rate of 55 rpm and treatment period of 60 min. Enzymatic treatment of sisal fibers enhanced the brightness and whiteness by 11.52 and 6.83%, respectively, and reduced the yellowness by 7.14% in comp...
Source: Bioprocess and Biosystems Engineering - October 8, 2020 Category: Biomedical Engineering Authors: Singh A, Varghese LM, Battan B, Patra AK, Mandhan RP, Mahajan R Tags: Bioprocess Biosyst Eng Source Type: research

Limited oxygen conditions as an approach to scale-up and improve D and L-lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineered Escherichia coli.
Martinez A Abstract The effectiveness of micro-aeration on lactate (LA) production by metabolically engineered Escherichia coli was evaluated in 1 L bioreactors containing mineral media and glucose (70 g/L). Volumetric oxygen transfer coefficients (kLa) between 12.6 and 28.7 h-1 increased the specific growth rate (µ) and volumetric productivity (QLA) by 300 and 400%, respectively, without a significant decrease in lactate yield (YLA), when compared with non-aerated fermentations. A kLa of 12.6 h-1 was successfully used as a criterion to scale-up the production of L and D-lactate from 1 t...
Source: Bioprocess and Biosystems Engineering - October 7, 2020 Category: Biomedical Engineering Authors: Sierra-Ibarra E, Leal-Reyes LJ, Huerta-Beristain G, Hernández-Orihuela AL, Gosset G, Martínez-Antonio A, Martinez A Tags: Bioprocess Biosyst Eng Source Type: research

Synthesis of propyl benzoate by solvent-free immobilized lipase-catalyzed transesterification: Optimization and kinetic modeling.
Abstract The present study aimed to analyze reaction kinetics and mechanism for the synthesis of propyl benzoate in solvent-free conditions. Lipase was immobilized on Hydroxypropyl methylcellulose (HPMC) and polyvinyl alcohol (PVA) polymer blend by entrapment method. Among different lipases immobilized on a support, Candida cylindracea (CCL) showed excellent activity. Systematic studies were done to optimize the reaction conditions. The activation energy was found to be 16.2 kcal/mol for immobilized CCL. Kinetic parameters were calculated, which depicted that propyl benzoate synthesized using immobilized CCL ...
Source: Bioprocess and Biosystems Engineering - September 29, 2020 Category: Biomedical Engineering Authors: Jawale PV, Bhanage BM Tags: Bioprocess Biosyst Eng Source Type: research

Hybrid neural network modeling and particle swarm optimization for improved ethanol production from cashew apple juice.
Cartaxo SJM Abstract A hybrid neural model (HNM) and particle swarm optimization (PSO) was used to optimize ethanol production by a flocculating yeast, grown on cashew apple juice. HNM was obtained by combining artificial neural network (ANN), which predicted reaction specific rates, to mass balance equations for substrate (S), product and biomass (X) concentration, being an alternative method for predicting the behavior of complex systems. ANNs training was conducted using an experimental set of data of X and S, temperature and stirring speed. The HNM was statistically validated against a new dataset, being capab...
Source: Bioprocess and Biosystems Engineering - September 28, 2020 Category: Biomedical Engineering Authors: da Silva Pereira A, Pinheiro ÁDT, Rocha MVP, Gonçalves LRB, Cartaxo SJM Tags: Bioprocess Biosyst Eng Source Type: research

Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors.
lik E Abstract Polyhydroxyalkanoates (PHAs) are biodegradable polyesters accumulated in a wide variety of microorganisms as intracellular carbon and energy storage compounds. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one of the most valuable biopolymers because of its superior mechanical properties. Here, we developed a bioprocess utilizing recombinant Bacillus megaterium strain for PHBV over-production from glucose, without any precursor addition. PHA production was performed in a controlled bioreactor by batch and fed-batch modes using wild-type B. megaterium and rec-B. megaterium cells overexpressi...
Source: Bioprocess and Biosystems Engineering - September 28, 2020 Category: Biomedical Engineering Authors: Akdoğan M, Çelik E Tags: Bioprocess Biosyst Eng Source Type: research

Nitrate removal performances of a new aerobic denitrifier, Acinetobacter haemolyticus ZYL, isolated from domestic wastewater.
Abstract A new aerobic denitrifying bacterium ZYL was isolated from domestic wastewater sludge and identified as Acinetobacter haemolyticus (similarity 99%) by the 16S rDNA sequencing analysis. The strain could use nitrate, nitrite and ammonium as the sole N-source for growth with a final product of N2, demonstrating its good abilities for aerobic denitrification and heterotrophic nitrification. Single-factor experiment results showed that the effective removal of nitrate by strain ZYL occurred with carbon source sodium succinate, C/N 16-24, pH 5-9, temperature 20-40 °C, DO ≥ 4.84 mg...
Source: Bioprocess and Biosystems Engineering - September 26, 2020 Category: Biomedical Engineering Authors: Wang Y, Zou YL, Chen H, Lv YK Tags: Bioprocess Biosyst Eng Source Type: research

Biohydrogen production in an AFBR using sugarcane molasses.
This study evaluated an anaerobic fluidized bed reactor to produce hydrogen from sugarcane molasses of 25 g-COD L-1. The reactor of 1.2 L working volume contained shredded tires as support material. The inoculum was sludge obtained in a UASB reactor of a sewage treatment plant. The AFBR was operated at hydraulic retention times of 12, 6, 4 and 3 h. The maximum hydrogen production rate (1.44 L-H2 h-1 L-1) and the highest hydrogen yield (3.07 mol-H2 mol-1-glucose) occurred at HRT of 4 and 6 h, respectively. The highest COD removal (23.3 ± 8.5%) was achiev...
Source: Bioprocess and Biosystems Engineering - September 25, 2020 Category: Biomedical Engineering Authors: Chaves TC, Gois GNSB, Peiter FS, Vich DV, de Amorim ELC Tags: Bioprocess Biosyst Eng Source Type: research

Enhancing electrochemical sensing for catechol by biomimetic oxidase covalently functionalized graphene oxide.
In this study, functionalized graphene oxide was synthesized by chemically modifying the surface of graphene oxide. The prepared carrier was covalently combined with biomimetic oxidase iron porphyrin (FePP, the active center of horseradish peroxidase). Ionic liquid as covalent coupling agents was designed as electronic bridge between biomimetic oxidase and graphene oxide. The novel biomimetic biosensor provided a detection range of 50.0-1600.0 μmol/L by modulating under the optimal conditions of the reaction system (FePP concentration is 1.5 × 10-3 mol/L, pH 3.0, Nafion solution dosage 1% a...
Source: Bioprocess and Biosystems Engineering - September 22, 2020 Category: Biomedical Engineering Authors: Jiaojiao X, Pengyun W, Bin Z, Onyinye AI Tags: Bioprocess Biosyst Eng Source Type: research

Enhancement of schizophyllan production in Schizophyllum commune using microparticles in medium.
In this study, adding talc and aluminium oxide microparticles into the cultivation medium was studied to improve the fungal growth and morphology. The response surface methodology and one factor at a time were applied to find the effects of microparticles with different sizes and concentrations on the schizophyllan production. The optimum concentration and size of aluminium oxide microparticles were obtained as 20 g L-1 and 
Source: Bioprocess and Biosystems Engineering - September 20, 2020 Category: Biomedical Engineering Authors: Alizadeh V, Shojaosadati SA, Zamir SM Tags: Bioprocess Biosyst Eng Source Type: research

Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source.
In this study, chitosan production through submerged fermentation of Aspergillus terreus on apple waste extract as sole carbon source was investigated. Monod equation with a maximum specific growth rate of 0.083 h-1 and half-saturation constant of 6.67 w/v% was best described the kinetic of growth. Results of response surface methodology showed the highest chitosan to substrate yield of 49.32 mg gsubstrate-1, chitosan to fungal biomass yield of 140.9 mg gcell-1, and fungal biomass to substrate yield of 0.387 gcell gsubstrate-1 were simultaneously obtained at temperature 30.0 °C, initial pH 5.98, and...
Source: Bioprocess and Biosystems Engineering - September 20, 2020 Category: Biomedical Engineering Authors: Habibi A, Karami S, Varmira K, Hadadi M Tags: Bioprocess Biosyst Eng Source Type: research

Directed evolution of Aspergillus oryzae lipase for the efficient resolution of (R,S)-ethyl-2-(4-hydroxyphenoxy) propanoate.
In this study, a mutant lipase AOL-3F38N/V230R was screened through two rounds of directed evolution, resulting in a fourfold increase in lipase activity, and threefold in catalytic efficiency (kcat/Km), while maintaining its excellent stereoselectivity. AOL-3F38N/V230R enzyme activity was maximum at pH 7.5 and also at 40 °C. And compared with wild-type AOL-3, AOL-3F38N/V230R preferentially hydrolyzed the fatty acid ethyl ester carbon chain length from C4 to C6-C10. In the same catalytic reaction conditions, the conversion of (R,S)-ethyl-2-(4-hydroxyphenoxy) propanoate ((R,S)-EHPP) by AOL-3F38N/V230R can be increa...
Source: Bioprocess and Biosystems Engineering - September 20, 2020 Category: Biomedical Engineering Authors: Zhang M, Li Q, Lan X, Li X, Zhang Y, Wang Z, Zheng J Tags: Bioprocess Biosyst Eng Source Type: research

Bacterial community analysis of biofilm on API 5LX carbon steel in an oil reservoir environment.
This study aimed to characterize the biofilm microbial community that causes corrosion of API 5LX carbon steel. API 5LX carbon steel coupons were incubated with raw produced water collected from two oil reservoir stations or filter-sterilized produced water. Biofilm 16S rRNA amplicon sequencing revealed that the bacterial community present in the biofilm was dominated by Proteobacteria, including Marinobacter hydrocarbonoclaustics and Marinobacter alkaliphilus. Electrochemical analysis such as impedance and polarization results indicated that Proteobacteria biofilm accelerated corrosion by ~ twofold (2.1&thin...
Source: Bioprocess and Biosystems Engineering - September 20, 2020 Category: Biomedical Engineering Authors: Elumalai P, AlSalhi MS, Mehariya S, Karthikeyan OP, Devanesan S, Parthipan P, Rajasekar A Tags: Bioprocess Biosyst Eng Source Type: research

Deconstruction of banana peel for carbohydrate fractionation.
, Poletto P Abstract The deconstruction of banana peel for carbohydrate recovery was performed by sequential treatment (acid, alkaline, and enzymatic). The pretreatment with citric acid promoted the extraction of pectin, resulting in a yield of 8%. In addition, xylose and XOS, 348.5 and 17.3 mg/g xylan, respectively, were also quantified in acidic liquor as a result of partial depolymerization of hemicellulose. The spent solid was pretreated with alkaline solution (NaOH or KOH) for delignification and release of residual carbohydrates from the hemicellulose. The yields of xylose and arabinose (225.2 and 174.0...
Source: Bioprocess and Biosystems Engineering - September 17, 2020 Category: Biomedical Engineering Authors: Pereira MAF, Monteiro CRM, Pereira GN, Júnior SEB, Zanella E, Ávila PF, Stambuk BU, Goldbeck R, de Oliveira D, Poletto P Tags: Bioprocess Biosyst Eng Source Type: research

Efficient biosynthesis of polysaccharide welan gum in heat shock protein-overproducing Sphingomonas sp. via temperature-dependent strategy.
In this study, a temperature-dependent strategy was designed for two-stage welan production through overexpressing heat shock proteins in Sphingomonas sp. The first stage was cell growth phase with higher TCA cycle activity at 42 °C; the second stage was welan formation phase with higher precursor synthesis pathway activity at 37 °C. The highest welan concentration 37.5 g/L was achieved after two-stage process. Ultimately, this strategy accumulated welan yield of 79.2 g/100 g glucose and productivity of 0.62 g/L/h at 60 h, which were the best reported results so far. The duration ...
Source: Bioprocess and Biosystems Engineering - September 16, 2020 Category: Biomedical Engineering Authors: Zhu P, Zhan Y, Wang C, Liu X, Liu L, Xu H Tags: Bioprocess Biosyst Eng Source Type: research

A kinetic study on carboxylic acids production using bovine slaughterhouse wastewater: a promising substrate for resource recovery in biotechnological processes.
os AB Abstract Carboxylic acids (CA) are considered high added-value compounds, and their production from wastes has gained economic and environmental notoriety. However, the CA production and kinetic modeling using some agro-industrial wastewaters, such as bovine slaughterhouse wastewater (SHW), are not well reported in the literature. Therefore, the objective of this work was to evaluate the CA production potential using SHW as a substrate under acidogenic conditions and to apply mathematical models to estimate the kinetic parameters of particulate organic matter hydrolysis, soluble organic matter consumption, a...
Source: Bioprocess and Biosystems Engineering - September 10, 2020 Category: Biomedical Engineering Authors: Morais NWS, Coelho MMH, Ferreira TJT, Pereira EL, Leitão RC, Dos Santos AB Tags: Bioprocess Biosyst Eng Source Type: research

Fed-batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arabinose.
In this study, fed-batch mode was applied to produce PHB on three sugar mixtures (glucose:xylose:arabinose = 4:2:1, 2:2:1, 1:2:1). The highest PHB concentration produced was 67 g/L for 4:2:1 mixture at 41 h corresponding to an accumulation of 77% of cell dry weight as PHB. Corresponding sugar conversion efficiency and productivity were 0.33 g PHB/g sugar consumed and 1.6 g/L/h, respectively. The results provide references for process control to maximize PHB production from real sugar streams derived from corn fibre. PMID: 32895870 [PubMed - as supplied by publisher] (Source: Bioproces...
Source: Bioprocess and Biosystems Engineering - September 6, 2020 Category: Biomedical Engineering Authors: Li M, Wilkins MR Tags: Bioprocess Biosyst Eng Source Type: research

A selected bacterial strain for the self-healing process in cementitious specimens without cell immobilization steps.
za RL Abstract The use of microorganisms capable of mediating the bioprecipitation process can be an important application in the self-healing processes of cement specimens. Thus, the present study identified and evaluated five Bacillus strains for potential application in the protocol of self-healing via bioprecipitation. Cell growth, enzyme production, and kinetic parameters conditions were evaluated during the fermentation process. Based on the analysis of 16S rDNA in conjunction with biochemical testing, results demonstrate that the strains are either Bacillus cereus or Bacillus thuringiensis. Strategically it...
Source: Bioprocess and Biosystems Engineering - September 4, 2020 Category: Biomedical Engineering Authors: Santos RP, Ramos TM, Borges BM, Hollanda LM, Lima ÁS, Soares CMF, Souza RL Tags: Bioprocess Biosyst Eng Source Type: research

Evaluation of the production of alginate-like exopolysaccharides (ALE) and tryptophan in aerobic granular sludge systems.
Abstract The engineering and microbiological aspects involved in the production of alginate-like exopolysaccharides (ALE) and tryptophan (TRY) in aerobic granular sludge systems were evaluated. The inclusion of short anoxic phase (A/O/A cycle-anaerobic, oxic, and anoxic phase) and the control of sludge retention time (SRT ≈ 10 days) proved to be an important strategy to increase the content of these bioproducts in granules. The substrate concentration also has a relevant impact on the production of ALE and TRY. The results of the microbiological analysis showed that slow-growing heterotrophic ...
Source: Bioprocess and Biosystems Engineering - September 4, 2020 Category: Biomedical Engineering Authors: Rollemberg SLS, Dos Santos AF, Ferreira TJT, Firmino PIM, Dos Santos AB Tags: Bioprocess Biosyst Eng Source Type: research

Efficacy of red light for enhanced cell disruption and fluorescence intensity of phycocyanin.
In this study, red LED and urea used as light and nitrogen sources, respectively, for the cultivation of Spirulina to enhance the fluorescence property and purity of phycocyanin. Besides, there is a high concentration of phycocyanin leached out from red light (RL) grown cells than white light (WL) without cell disruption. This type of cultivation reduces the complexity of extraction methods and cost of the downstream process. The fluorescence intensity of C-PC enhanced while using red LEDs and purity ratio improved by single-step cation exchange chromatography. Phycocyanin from red-light-exposed culture exhibited pronounce...
Source: Bioprocess and Biosystems Engineering - September 3, 2020 Category: Biomedical Engineering Authors: Sivasankari S, Vinoth M, Ravindran D, Baskar K, Alqarawi AA, Abd Allah EF Tags: Bioprocess Biosyst Eng Source Type: research

Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05T extracellular proteases.
This study provided valuable information for the exploitation of PAT 05T proteases which have potential for the valorization of organic wool as well as for other industrial applications. PMID: 32888092 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - September 3, 2020 Category: Biomedical Engineering Authors: Olivera NL, Sequeiros C, Iglesias M, Nievas M Tags: Bioprocess Biosyst Eng Source Type: research

Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium.
Abstract Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosus LRa05, L. casei LC89, L. plantarum N13, L. acidophilus LA85, and L. brevis LB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside). The enzymatic activity, metabolites, antioxidant activities, and α-glucosidase inhibition property in the two standards were also investigated before and after ...
Source: Bioprocess and Biosystems Engineering - September 3, 2020 Category: Biomedical Engineering Authors: Guo X, Guo A, Li E Tags: Bioprocess Biosyst Eng Source Type: research

Investigating the potential of locally sourced wastewater as a feedstock of microbial desalination cell (MDC) for bioenergy production.
Abstract Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells (MDCs) represent a sustainable option that has added benefit of solving the ever-increasing wastewater treatment and management problem. MDCs are a customized type of Microbial Fuel Cells (MFCs) that depend on the electric potential generated by organic media to decrease salt concentration by...
Source: Bioprocess and Biosystems Engineering - August 31, 2020 Category: Biomedical Engineering Authors: Liaquat R, Mehmood T, Khoja AH, Iqbal N, Ejaz H, Mumtaz S Tags: Bioprocess Biosyst Eng Source Type: research

Bioprocessing and integration of a high flux screening systematic platform based on isothermal amplification for the detection on 8 common pathogens.
In this study, a high flux screening based on nucleic acid isothermal amplification technique has been developed. For the 8 common pathogens, species-specific targets had been selected and analyzed for their unique specificity. After optimization, separate LAMP reaction assays had been bioprocessed and integrated into one systematic detection platform, including 8 strips (PCR tubes) and 96-well plates. Eight standard strains verified for the accuracy. Application of the established high flux screening platform was used for detection for 48 samples in 4 different 96-well plates, with 2 groups of 2 operators using double-bli...
Source: Bioprocess and Biosystems Engineering - August 28, 2020 Category: Biomedical Engineering Authors: Zhong H, Deng H, Li M, Zhong H Tags: Bioprocess Biosyst Eng Source Type: research

The effect of Np-magnetite on the granulation process of an SBR reactor used for domestic wastewater treatment.
This study investigated the effect of magnetite nanoparticles (Np-magnetite) added to a pilot-scale sequencing batch reactor (SBR) treating domestic wastewater, to improve aerobic granular sludge (AGS) formation and the effects of granule disintegration. Np-magnetite additions (75 mg L-1) were made during the start-up of the reactor and repeated after 100 and 170 days, when granule disintegration was observed. From the first Np-magnetite addition, SVI5 was reduced from 1315 to 85 mL g-1. The granular biomass was observed on the 56th day, when 57% of the granules presented diameters bigger than 212 ...
Source: Bioprocess and Biosystems Engineering - August 28, 2020 Category: Biomedical Engineering Authors: Domingos DG, Libardi N, Henriques RO, Xavier JA, da Costa RHR Tags: Bioprocess Biosyst Eng Source Type: research

Reaction coupling separation for isosteviol production from stevioside catalyzed by acidic ion-exchange resin.
Abstract Isosteviol, a prodrug used to be obtained via Wagner-Meerwein rearrangement from steviol with low yield and long reaction time. Herein, an in-situ separation-coupling-reaction is presented to prepare isosteviol from the natural sweetener stevioside. Simply with in-situ water-washing, the product containing 92.98% purity of isosteviol was obtained with a stevioside conversion of 97.23% from a packet bed reactor without further separation. Within the assayed inorganic acid, organic acids and acidic ionic liquids, the acidic ion-exchange resins provided higher product specificity towards isosteviol. Fur...
Source: Bioprocess and Biosystems Engineering - August 27, 2020 Category: Biomedical Engineering Authors: Hu X, Zhou Z, Zhang Z, Wang X, Sui X, Chen J, Xia Y, Zhang J, Lin J Tags: Bioprocess Biosyst Eng Source Type: research

Assessment and evaluation of cellulase production using ragi (Eleusine coracana) husk as a substrate from thermo-acidophilic Aspergillus fumigatus JCM 10253.
Abstract The cellulase production by filamentous fungi Aspergillus fumigatus JCM 10253 was carried out using agro-industrial waste ragi husk as a substrate in the microbial fermentation. The effect of the process parameters such as temperature, substrate concentration, pH, and incubation process time and their interdependence was studied using response surface methodology. The optimum cellulase activities were obtained at 50 °C under the conditions with 1-2% of substrate concentration at pH 2-4 for the incubation period of 7-8 days. The maximum carboxymethyl cellulase (CMCase) and β-glucosidase ...
Source: Bioprocess and Biosystems Engineering - August 25, 2020 Category: Biomedical Engineering Authors: Saroj P, P M, Narasimhulu K Tags: Bioprocess Biosyst Eng Source Type: research

Mass transfer in aerated culture media combining mixed electrolytes and glucose.
dríguez SA Abstract The combined effects of mixed electrolyte species and glucose on oxygen transfer were studied in a bubble column with aqueous solutions. Of particular interest was the presence of electrolytes containing ions which are prone to present solute-solute interactions or to crystallize. Without and at low concentration of glucose (≤ 5 g/L), the increasing concentration of electrolytes (nominal ionic strength: 0-0.43 M), up to a critical value, enhanced the volumetric mass transfer coefficient (kLa) and the availability of specific interfacial area (a), due to the inhibition...
Source: Bioprocess and Biosystems Engineering - August 24, 2020 Category: Biomedical Engineering Authors: Góngora-García OR, Aca-Aca G, Baz-Rodríguez SA Tags: Bioprocess Biosyst Eng Source Type: research

A scalable insect cell-based production process of the human recombinant BMX for in-vitro covalent ligand high-throughput screening.
e;o A Abstract Bone Marrow Tyrosine kinase in the chromosome X (BMX) is a TEC family kinase associated with numerous pathological pathways in cancer cells. Covalent inhibition of BMX activity holds promise as a therapeutic approach against cancer. To screen for potent and selective covalent BMX inhibitors, large quantities of highly pure BMX are normally required which is challenging with the currently available production and purification processes. Here, we developed a scalable production process for the human recombinant BMX (hrBMX) using the insect cell-baculovirus expression vector system. Comparable expressi...
Source: Bioprocess and Biosystems Engineering - August 19, 2020 Category: Biomedical Engineering Authors: Sousa BB, Sousa MFQ, Marques MC, Seixas JD, Brito JA, Matias PM, Bernardes GJL, Roldão A Tags: Bioprocess Biosyst Eng Source Type: research

Design of immobilized biocatalyst and optimal conditions for tyrosol β-galactoside production.
Design of immobilized biocatalyst and optimal conditions for tyrosol β-galactoside production. Bioprocess Biosyst Eng. 2020 Aug 20;: Authors: Hollá V, Hill R, Antošová M, Polakovič M Abstract Tyrosol β-galactoside (TG) is a phenylethanoid glycoside with proven neuroprotective properties. This work deals with its biocatalytic production from tyrosol and lactose using Aspergillus oryzae β-galactosidase in immobilized form. Six commercial carriers were examined to find the optimal biocatalyst. Besides standard biocatalyst performance characteristics, adsorption of the...
Source: Bioprocess and Biosystems Engineering - August 19, 2020 Category: Biomedical Engineering Authors: Hollá V, Hill R, Antošová M, Polakovič M Tags: Bioprocess Biosyst Eng Source Type: research

Feedback control of two supplemental feeds during fed-batch culture on a platform process using inline Raman models for glucose and phenylalanine concentration.
Abstract The use of Raman models for glucose and phenylalanine concentrations to provide the signal for a control algorithm to continuously adjust the feed rate of two separate supplemental feeds during the fed-batch culture of a CHOK1SV GS-KO® cell line in a platform process was evaluated. Automated feed rate adjustment of the glucose feed using a Raman model for glucose concentration, maintained the glucose concentration within the desired target (average deviation ± 0.49 g/L). Automated feed rate adjustment of the nutrient feed using a Raman model for phenylalanine concentration, m...
Source: Bioprocess and Biosystems Engineering - August 19, 2020 Category: Biomedical Engineering Authors: Webster TA, Hadley BC, Dickson M, Busa JK, Jaques C, Mason C Tags: Bioprocess Biosyst Eng Source Type: research

Exploring the potential of halotolerant bacteria for biodegradation of polycyclic aromatic hydrocarbon.
Abstract The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations...
Source: Bioprocess and Biosystems Engineering - August 18, 2020 Category: Biomedical Engineering Authors: Al Farraj DA, Hadibarata T, Yuniarto A, Alkufeidy RM, Alshammari MK, Syafiuddin A Tags: Bioprocess Biosyst Eng Source Type: research