Analysis of the use of a non-conventional rotary drum for dehydration of microalga Spirulina platensis.
Abstract The high content of bioactive compounds in the microalga Spirulina platensis has recently attracted attention from food and pharmaceutical industries. However, for its application an effective preservation technique must be developed. In this paper, we investigated the use of a non-conventional rotary dryer (with an inert bed) for drying the microalga Spirulina biomass and the effects of the operational conditions (air temperature, intermittent feeding interval, filling degree of inert particles, and rotation speed) on its bioactive compounds. The results indicated that this non-conventional drying system...
Source: Bioprocess and Biosystems Engineering - March 26, 2020 Category: Biomedical Engineering Authors: Silva NC, Duarte CR, Barrozo MAS Tags: Bioprocess Biosyst Eng Source Type: research

Malassezia and Staphylococcus dominate scalp microbiome for seborrheic dermatitis.
Abstract Seborrheic dermatitis (SD) is a common disease of the human scalp that causes physical damage and psychological problems for patients. Studies have indicated that dysbiosis of the scalp microbiome results in SD. However, the specific fungal and bacterial microbiome changes related to SD remain elusive. To further investigate the fungal and bacterial microbiome changes associated with SD, we recruited 57 SD patients and 53 healthy individuals and explored their scalp microbiomes using next generation sequencing and the QIIME and LEfSe bioinformatics tools. Skin pH, sebum secretion, hydration, and trans-epi...
Source: Bioprocess and Biosystems Engineering - March 26, 2020 Category: Biomedical Engineering Authors: Lin Q, Panchamukhi A, Li P, Shan W, Zhou H, Hou L, Chen W Tags: Bioprocess Biosyst Eng Source Type: research

A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production.
In this study, a genome-scale metabolic network model of CHO cells was used to design feeding strategies for CHO cells to improve monoclonal antibody (mAb) production. A number of metabolites, including threonine and arachidonate, were suggested by the model to be added into cell culture medium. The designed composition has been experimentally validated, and then optimized, using design of experiment methods. About a two-fold increase in the total mAb expression has been observed using this strategy. Our approach can be used in similar bioprocess optimization problems, to suggest new ways of increasing production in differ...
Source: Bioprocess and Biosystems Engineering - March 24, 2020 Category: Biomedical Engineering Authors: Fouladiha H, Marashi SA, Torkashvand F, Mahboudi F, Lewis NE, Vaziri B Tags: Bioprocess Biosyst Eng Source Type: research

Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors.
Abstract Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 °C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L-1 min-1 in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operatio...
Source: Bioprocess and Biosystems Engineering - March 21, 2020 Category: Biomedical Engineering Authors: Castiglioni GZ, Bettio G, Matte CR, Jacques RA, Dos Santos Polidoro A, Rosa CA, Ayub MAZ Tags: Bioprocess Biosyst Eng Source Type: research

Comparison of anaerobic digesters performance treating palmitic, stearic and oleic acid: determination of the LCFA kinetic constants using ADM1.
Abstract In this work, the process performance of three parallel anaerobic digesters was evaluated while treating emulsified long-chain fatty acids (LCFA) (i.e., palmitic, stearic and oleic acid) at two different organic loading rates (OLR) (2 and 4 g L-1 day-1). The digester treating palmitic acid achieved a biogas yield of 0.42 ± 0.05 L g-1 COD with a digester effluent COD of 2.3 ± 0.4 g L-1 and VFA concentrations below 250 mg L-1. Stearic acid showed lower biogas yield values (0.34 ± 0.05 L g-1 COD) with low effluent COD (2.5 &...
Source: Bioprocess and Biosystems Engineering - March 20, 2020 Category: Biomedical Engineering Authors: Eftaxias A, Diamantis V, Michailidis C, Stamatelatou K, Aivasidis A Tags: Bioprocess Biosyst Eng Source Type: research

Characterization of two new aromatic amino acid lyases from actinomycetes for highly efficient production of p-coumaric acid.
Abstract p-Coumaric acid (p-CA) is a bioactive natural product and an important industrial material for pharmaceuticals and nutraceuticals. It can be synthesized from deamination of L-tyrosine by tyrosine ammonia lyase (TAL). In this work, we discovered two aromatic amino acid lyase genes, Sas-tal and Sts-tal, from Saccharothrix sp. NRRL B-16348 and Streptomyces sp. NRRL F-4489, respectively, and expressed them in Escherichia coli BL21(DE3). The two enzymes were functionally characterized as TAL. The optimum reaction temperature for Sas-TAL and Sts-TAL is 55 °C and 50 °C, respectively; while, the...
Source: Bioprocess and Biosystems Engineering - March 20, 2020 Category: Biomedical Engineering Authors: Cui P, Zhong W, Qin Y, Tao F, Wang W, Zhan J Tags: Bioprocess Biosyst Eng Source Type: research

Investigation of the reversibility of freeze/thaw stress-induced protein instability using heat cycling as a function of different cryoprotectants.
This study aims to evaluate the addition of a heat cycle following FT application as a function of different cryoprotectants with lysozyme as exemplary protein. Sucrose and glycerol were shown to be the most effective cryoprotectants when compared to PEG200 and Tween20. In terms of heat-induced reversibility of aggregates, glycerol showed the best performance followed by sucrose, NaCl and Tween20 systems. The analysis was performed using a novel approach to visualize complex interplays by a clustering and data reduction scheme. In addition, solubility and structural integrity were measured and confirmed the obtained result...
Source: Bioprocess and Biosystems Engineering - March 20, 2020 Category: Biomedical Engineering Authors: Wöll AK, Hubbuch J Tags: Bioprocess Biosyst Eng Source Type: research

Upscale production of (R)-mandelic acid with a stereospecific nitrilase in an aqueous system.
This study paved the foundation for the upscale production of R-MA using E. coli whole cells as biocatalyst. PMID: 32193756 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - March 20, 2020 Category: Biomedical Engineering Authors: Zhang XH, Wang CY, Cai X, Xue YP, Liu ZQ, Zheng YG Tags: Bioprocess Biosyst Eng Source Type: research

Low-cost novel clay earthenware as separator in microbial electrochemical technology for power output improvement.
In this study, a novel porous clay earthenware (NCE) was fabricated as the separator to replace the highly cost PEM. The fabrication of NCEs is with raw clay powder and starch powder that acts as a pore-forming agent at different starch powder contents (10 vol%, 20 vol%, and 30 vol%), ball-milled before hydraulically pressed to form green ceramic pellets and sintered up to 1200 °C. The highest power density of 2250 ± 21 mW/m2 (6.0 A/m2), the internal resistance of 75 ± 24 Ω and coulombic efficiency (CE) of 44 ± 21% were produced fo...
Source: Bioprocess and Biosystems Engineering - March 19, 2020 Category: Biomedical Engineering Authors: Daud SM, Daud WRW, Bakar MHA, Kim BH, Somalu MR, Muchtar A, Jahim JM, Muhammed Ali SA Tags: Bioprocess Biosyst Eng Source Type: research

Selected nanotechnologies and nanostructures for drug delivery, nanomedicine and cure.
Abstract The development of nanoparticle-based drugs has provided many opportunities to diagnose, treat and cure challenging diseases. Through the manipulation of size, morphology, surface modification, surface characteristics, and materials used, a variety of nanostructures can be developed into smart systems, encasing therapeutic and imaging agents with stealth properties. These nanostructures can deliver drugs to specific tissues or sites and provide controlled release therapy. This targeted and sustained drug delivery decreases the drug-related toxicity and increases the patient's compliance with less frequent...
Source: Bioprocess and Biosystems Engineering - March 19, 2020 Category: Biomedical Engineering Authors: Khan AU, Khan M, Cho MH, Khan MM Tags: Bioprocess Biosyst Eng Source Type: research

Immobilization of lipase Eversa Transform 2.0 on poly(urea-urethane) nanoparticles obtained using a biopolyol from enzymatic glycerolysis.
ira D Abstract In this work, the free lipase Eversa® Transform 2.0 was used as a catalyst for enzymatic glycerolysis reaction in a solvent-free system. The product was evaluated by nuclear magnetic resonance (1H NMR) and showed high conversion related to hydroxyl groups. In sequence, the product of the glycerolysis was used as stabilizer and biopolyol for the synthesis of poly(urea-urethane) nanoparticles (PUU NPs) aqueous dispersion by the miniemulsion polymerization technique, without the use of a further surfactant in the system. Reactions resulted in stable dispersions of PUU NPs with an average diameter o...
Source: Bioprocess and Biosystems Engineering - March 18, 2020 Category: Biomedical Engineering Authors: Bresolin D, Hawerroth B, de Oliveira Romera C, Sayer C, de Araújo PHH, de Oliveira D Tags: Bioprocess Biosyst Eng Source Type: research

Evaluation of ethanol fermentation efficiency of sweet sorghum syrups produced by integrated dual-membrane system.
Abstract 1G ethanol from sweet sorghum can be a better alternative to various other sources used for its production. The commercial feasibility is dictated by the high sugar containing varieties, their transport to ethanol plants, storage and availability of robust yeast strains for the fermentation. Eight sweet sorghum cultivars namely CSV19SS, CSV24SS, CSV27, CSV32F, PV, SSV84, RVICSH, SPV1871, SSV74 were tested for their sugar content and varieties-SSV84 and CSV24SS were containing sugar content of 170-190 g/L. Specifically designed polyethersulfone-based ultrafiltration (UF) membrane and hydrophilised pol...
Source: Bioprocess and Biosystems Engineering - March 14, 2020 Category: Biomedical Engineering Authors: Kanakaraju Y, Uma A, Vani G, Kiran Kumari P, Sridhar S, Umakanth AV Tags: Bioprocess Biosyst Eng Source Type: research

Effect of shear on morphology, viability and metabolic activity of succinic acid-producing Actinobacillus succinogenes biofilms.
Abstract Two custom-designed bioreactors were used to evaluate the effect of shear on biofilms of a succinic acid producer, Actinobacillus succinogenes. The first bioreactor allowed for in situ removal of small biofilm samples used for microscopic imaging. The second bioreactor allowed for complete removal of all biofilm and was used to analyse biofilm composition and productivity. The smooth, low porosity biofilms obtained under high shear conditions had an average cell viability of 79% compared to 57% at the lowest shear used. The maximum cell-based succinic acid productivity for high shear biofilm was 2.4 ...
Source: Bioprocess and Biosystems Engineering - March 14, 2020 Category: Biomedical Engineering Authors: Mokwatlo SC, Brink HG, Nicol W Tags: Bioprocess Biosyst Eng Source Type: research

High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process.
Abstract Lactobionic acid and sorbitol are produced from lactose and fructose in reactions catalyzed by glucose-fructose oxidoreductase and glucono-δ-lactonase, periplasmic enzymes present in Zymomonas mobilis cells. Considering the previously established laboratory-scale process parameters, the bioproduction of lactobionic acid was explored to enable the transfer of this technology to the productive sector. Aspects such as pH, temperature, reuse and storage conditions of Ca-alginate immobilized Z. mobilis cells, and large-scale bioconversion were assessed. Greatest catalyst performance was observed between ...
Source: Bioprocess and Biosystems Engineering - March 14, 2020 Category: Biomedical Engineering Authors: Carra S, Rodrigues DC, Beraldo NMC, Folle AB, Delagustin MG, de Souza BC, Reginatto C, Polidoro TA, da Silveira MM, Bassani VL, Malvessi E Tags: Bioprocess Biosyst Eng Source Type: research

Simultaneous nitrogen and carbon removal in a packed A/O reactor: effect of C/N ratio on microbial community structure.
This study would be vital for a better understanding of microbial structures within a packed A/O MBBR and the development of cost-efficient strategies for the treatment of low C/N wastewater. PMID: 32166398 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - March 12, 2020 Category: Biomedical Engineering Authors: Ding S, He J, Luo X, Zheng Z Tags: Bioprocess Biosyst Eng Source Type: research

A comprehensive model of N2O emissions in an anaerobic/oxygen-limited aerobic process under dynamic conditions.
This study is expected to provide a meaningful reference for reducing N2O emissions in wastewater treatment engineering. PMID: 32166399 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - March 12, 2020 Category: Biomedical Engineering Authors: Li Z, Wan J, Ma Y, Wang Y, Huang Y, Fan H Tags: Bioprocess Biosyst Eng Source Type: research

Characterization on gut microbiome of PCOS rats and its further design by shifts in high-fat diet and dihydrotestosterone induction in PCOS rats.
Abstract Polycystic ovary syndrome (PCOS) is associated with gut microbiota disturbance. Emerging evidence has shown that gut microbiota plays a major role in the development of PCOS. To better understand how the gut microbiota contributes to the development of PCOS, we investigated the influences of high-fat diet and hyperandrogenism, independently or synergistically, have on the gut microbiota in rats. Furthermore, we explored the associations between gut microbiota and hyperandrogenism or other hallmarks of PCOS. Twenty female SD rats were randomized at aged 3 weeks into 4 groups (n = 5, each...
Source: Bioprocess and Biosystems Engineering - March 10, 2020 Category: Biomedical Engineering Authors: Zheng Y, Yu J, Liang C, Li S, Wen X, Li Y Tags: Bioprocess Biosyst Eng Source Type: research

Magnetic fields: biomass potential of Spirulina sp. for food supplement.
This study evaluated the influence of the magnetic field on the chemical composition of Spirulina sp. LEB 18 and its digestibility and protein solubility. The highest protein digestibility of biomass was obtained at 30 °C and with 2.5 g L-1 NaNO3 (78.4%) in the medium, and the highest solubility was found in the cultivated biomass exposed to 60 mT, 30 °C and 2.5 g L-1 NaNO3 (89%, pH 6). MF application did not modify the protein concentration of biomass, but reduced the carbohydrate concentration by 69.1%, showing that the biomass obtained in the culture submitted to MF may be used as an ingredie...
Source: Bioprocess and Biosystems Engineering - March 6, 2020 Category: Biomedical Engineering Authors: Veiga MC, Fontoura MM, de Oliveira MG, Costa JAV, Santos LO Tags: Bioprocess Biosyst Eng Source Type: research

Whole-cell bioconversion of naringenin to high added value hydroxylated compounds using Yarrowia lipolytica 2.2ab in surface and liquid cultures.
ha Guzmán NE, Huerta-Ochoa S Abstract The bioconversion process of bioactive naringenin by whole-cells of Yarrowia lipolytica 2.2ab for the production of increased value-added compounds is successfully achieved in surface and liquid cultures. This approach is an alternative to the commercial production of these bioactive compounds from vegetable sources, which are limited due to their low concentrations and the complexity of the purification processes. The experimentation rendered seven value-added compounds in both surface and liquid bioconversion cultures. Some of the compounds produced have not been prev...
Source: Bioprocess and Biosystems Engineering - March 6, 2020 Category: Biomedical Engineering Authors: Hernández-Guzmán C, Prado-Barragán A, Gimeno M, Román-Guerrero A, Rutiaga-Quiñones OM, Rocha Guzmán NE, Huerta-Ochoa S Tags: Bioprocess Biosyst Eng Source Type: research

Correction to: Semi-automation of process analytics reduces operator effect.
bauer A Abstract In the original publication, open access funding institution was incorrectly mentioned in the acknowledgement section. PMID: 32144596 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - March 6, 2020 Category: Biomedical Engineering Authors: Christler A, Felföldi E, Mosor M, Sauer D, Walch N, Dürauer A, Jungbauer A Tags: Bioprocess Biosyst Eng Source Type: research

Tragacanth-mediate synthesis of NiO nanosheets for cytotoxicity and photocatalytic degradation of organic dyes.
In this study, NiO nanosheets have been manufactured using a co-precipitation approach that involved the usage of nickel nitrate (Ni (NO3)2.6H2O) as the raw material and tragacanth in the role of a stabilizing agent. NiO nanosheets have been fabricated through the reduction of nickel nitrate solution that had been obtained by the application of aqueous extract of tragacanth, which is capable of functioning as a reducing and stabilizing agent. In the following, the physical and chemical properties of tragacanth-stabilized NiO nanosheets have been identified via FESEM, EDS, XRD, UV-Vis, and FT-IR techniques. According to the...
Source: Bioprocess and Biosystems Engineering - March 6, 2020 Category: Biomedical Engineering Authors: Sabouri Z, Akbari A, Hosseini HA, Khatami M, Darroudi M Tags: Bioprocess Biosyst Eng Source Type: research

Transformation of ginsenoside via deep eutectic solvents based on choline chloride as an enzymatic reaction medium.
Abstract Ginsenoside compound K (CK) with a wide range of pharmacological activities has been widely used in the healthcare product industry. However, the application of CK is limited by low productivity and difficult separation. The purpose of this study is to convert ginsenoside Rb1 into CK by improving conversion efficiency in novel "green" reaction medium-deep eutectic solvent (DES). Talaromyces purpureogenus was selected from ginseng rhizosphere soil to produce β-glucosidase with high activity and purity to transform ginsenosides, and Mn2+ was found to be an enzyme promoter. Among the DES based...
Source: Bioprocess and Biosystems Engineering - March 5, 2020 Category: Biomedical Engineering Authors: Ma Z, Mi Y, Han X, Li H, Tian M, Duan Z, Fan D, Ma P Tags: Bioprocess Biosyst Eng Source Type: research

Characterization of sludge reduction and bacterial community dynamics in a pilot-scale multi-stage digester system with prolonged sludge retention time.
Abstract Sludge reduction performance and bacterial community dynamics in a pilot-scale multi-stage digester system with prolonged sludge retention time were characterized. Throughout the operation period of 281 days, the total loading sludge and the total digested sludge were 4700 and 3300 kg-MLSS. After 114 days of operation, the residual MLSS (RMLSS) in the reactors for sludge treatment was maintained at 18-25 kg-RMLSS m-3, and the sludge reduction efficiency achieved 95% under the F/M ratio (kg-loading MLSS kg-RMLSS-1) of less than 0.018. Also, among the sludge components, both fixed suspen...
Source: Bioprocess and Biosystems Engineering - February 28, 2020 Category: Biomedical Engineering Authors: Yun J, Ryu HW, Kim HP, Cho KS Tags: Bioprocess Biosyst Eng Source Type: research

Multiple crystallization as a potential strategy for efficient recovery of succinic acid following fermentation with immobilized cells.
This study aimed to enhance the crystallizability of bio-based succinic acid for its efficient recovery while maintaining the end product at the highest purity. Immobilization of Actinobacillus succinogenes was initially evaluated based on three different carriers: volcanic glass, clay pebbles, and silica particles. The adsorption capacity of metabolites with a low concentration (10 g/L) and a high concentration (40 g/L) was investigated. It was demonstrated that clay pebbles adsorbed the least succinic acid (
Source: Bioprocess and Biosystems Engineering - February 24, 2020 Category: Biomedical Engineering Authors: Luthfi AAI, Tan JP, Isa NFAM, Bukhari NA, Shah SSM, Mahmod SS, Jahim JM Tags: Bioprocess Biosyst Eng Source Type: research

Evaluation of cytotoxicity of nanolipid carriers with structured Buriti oil in the Caco-2 and HepG2 cell lines.
do JA Abstract Buriti oil is rich in monounsaturated fatty acids, carotenoids and tocopherols and it is used for the treatment of various diseases. One strategy to restructure the triglycerides is enzymatic interesterification and nanocarriers have been employed to improve the solubility, bioavailability and stability of active compounds. This work aims to investigate the in vitro cytotoxicity of this structured oil in nanoemulsions and nanostructured lipid carriers to expand the applicability of the crude oil. None of the samples had a cytotoxic effect on Caco-2 and HepG2 cell lines at the concentrations tested. ...
Source: Bioprocess and Biosystems Engineering - February 20, 2020 Category: Biomedical Engineering Authors: Reis LVC, Leão KMM, Ribeiro APB, de Jesus MB, Macedo GA, Macedo JA Tags: Bioprocess Biosyst Eng Source Type: research

The high-efficient production of phelligridin LA by Inonotus baumii with an integrated fermentation-separation process.
This study provided a feasible approach for the improved production of PLA by fermentation. Besides, the design of the integrated bioreactor system offered a useful reference for the fermentation process development of fungi for the production of diverse valuable metabolites. PMID: 32078046 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - February 20, 2020 Category: Biomedical Engineering Authors: Wang T, Li H, Liang C, Sun S, Liu A, Zhu H Tags: Bioprocess Biosyst Eng Source Type: research

Enhanced production of bacterial xylanase and its utility in saccharification of sugarcane bagasse.
Abstract An investigation was carried out using sugarcane bagasse as the agricultural residue to study the optimization of xylanase production by solid-state fermentation. Maximum xylanase production (20.35 U/g substrate) was achieved by Bacillus substilis subsp. subtilis JJBS250 using 'one variable at a time approach' at pH 7.0, 40 °C after 48 h. After statistical optimization by response surface methodology (RSM) there was 4.82-fold improvement in xylanase production (98.16 U/g substrate). Further optimization of untreated and sodium carbonate pretreated sugarcane bagasse enzymatic hydrolysis was c...
Source: Bioprocess and Biosystems Engineering - February 17, 2020 Category: Biomedical Engineering Authors: Alokika, Singh B Tags: Bioprocess Biosyst Eng Source Type: research

A novel fungal laccase from Sordaria macrospora k-hell: expression, characterization, and application for lignin degradation.
Abstract The laccase has the ability to oxidize substituted phenols and the water is the sole byproduct, thus it has been employed to remove and/or modify the lignin in lignocellulosic material. A putative laccase gene, LacSM, from Sordaria macrospora k-hell was screened by a genome mining approach. Then, it was cloned and highly expressed in Escherichia coli. The molecular weight of recombinant LacSM was ~ 67 kDa. The optimal pH values for the LacSM oxidation of guaiacol, syringaldazine, 2,6-dimethoxyphenol, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were 6, 7, 5, and 5, respect...
Source: Bioprocess and Biosystems Engineering - February 17, 2020 Category: Biomedical Engineering Authors: Yang X, Gu C, Lin Y Tags: Bioprocess Biosyst Eng Source Type: research

High-level production and high-yield recovery of lactobionic acid by the control of pH and temperature in fermentation of Pseudomonas taetrolens.
Abstract Lactobionic acid (LBA) was produced by fermentation of Pseudomonas taetrolens. First, to increase the production of LBA by P. taetrolens, we controlled the pH of culture medium by CaCO3 addition (30 g/L) and then examined the initial lactose concentration ranging from 50 to 200 g/L and the growth temperature ranging from 20 to 37 °C. Both the LBA production titer (180 g/L) and the productivity (2.5 g/L h) were highest at 200 g/L lactose concentration and 25 °C of cell growth temperature in shake-flask culture. Although the production of LBA (178 g/L) wa...
Source: Bioprocess and Biosystems Engineering - February 15, 2020 Category: Biomedical Engineering Authors: Kim JH, Jang YA, Seong SB, Jang SA, Hong SH, Song JK, Eom GT Tags: Bioprocess Biosyst Eng Source Type: research

Co-cultures with integrated in situ product removal for lactate-based propionic acid production.
AP Abstract Propionic acid (PA) is a valuable organic acid for the food and feed industry, but no bioproduction at industrial scale exists so far. As product inhibition is a major burden for bioprocesses producing organic acids, in situ product removal (ISPR) is desirable. Here, we demonstrate a new strategy to produce PA with a co-culture coupled with ISPR using electrodialysis. Specifically, Bacillus coagulans first produces lactic acid (LA) from sugar(s) and LA is converted to PA using Veillonella criceti. Applying ISPR to the mentioned co-culture, the specific PA yield was increased from 0.35 to 0.39 g&n...
Source: Bioprocess and Biosystems Engineering - February 13, 2020 Category: Biomedical Engineering Authors: Selder L, Sabra W, Jürgensen N, Lakshmanan A, Zeng AP Tags: Bioprocess Biosyst Eng Source Type: research

Optimization of immobilization conditions for Lactobacillus penntosus cells.
In this study, the immobilization technology was used to improve the LA yield and shorten the fermentation time. The optimum conditions to immobilize Lactobacillus pentosus ATCC 8041 cell were determined by Taguchi design L16 (45). The immobilized L. pentosus ATCC 8041 cells prepared by 2% sodium alginate (SA) and 6% polyvinyl alcohol (PVA) with the immobilization process by 0.10 M calcium chloride (CaCl2) and 2.5% boric acid (H3BO3) had the best performance of LA yield at the temperature of 35 °C, which is significantly higher than that of L. pentosus ATCC 8041 free cells. These cells maintained the stable a...
Source: Bioprocess and Biosystems Engineering - February 8, 2020 Category: Biomedical Engineering Authors: Wang J, Huang J, Guo H, Jiang S, Zhang J, Ning Y, Fang M, Liu S Tags: Bioprocess Biosyst Eng Source Type: research

Steam explosion (SE) and instant controlled pressure drop (DIC) as thermo-hydro-mechanical pretreatment methods for bioethanol production.
Abstract Lignocellulosic biomass can be considered as one of the largest sources for the production of renewable biofuels (bioethanol). It involves an enzymatic treatment capable of ensuring the depolymerization of cellulose into fermentable sugars, followed by the production of ethanol by appropriate bacteriological fermentation. Proper destruction of the compact natural structure of the biomass would allow an interesting intensification of the operation. Among the most prominent technical approaches, the steam explosion (SE) is the most famous. However, this high pressure-high temperature process implies too hig...
Source: Bioprocess and Biosystems Engineering - February 6, 2020 Category: Biomedical Engineering Authors: Smichi N, Messaoudi Y, Allaf K, Gargouri M Tags: Bioprocess Biosyst Eng Source Type: research

Influence of galactosidases on glycosaminoglycan removal Vis- à-Vis opening up of skin matrix to enable complete rehydration.
In this study, galactosidases have been employed for rehydration of preserved skins and hides to improve the process efficiency and minimize hazardous sodium sulfide. The purpose of rehydration is to ensure the skin is hydrated uniformly to facilitate subsequent physico-chemical processes of leather making. Improper rehydration leads to reduction in the quality and value of the leather. The efficacy of the enzymatic process was studied using histological images and scanning electron microscopic analysis. Pollution load changes and the extent of carbohydrate removal were also quantified. The study indicates possibility for ...
Source: Bioprocess and Biosystems Engineering - February 5, 2020 Category: Biomedical Engineering Authors: Jayanthi D, Victor JS, Chellan R, Chellappa M Tags: Bioprocess Biosyst Eng Source Type: research

Alternative fertilizer-based growth media support high lipid contents without growth impairment in Scenedesmus obliquus BR003.
esi A Abstract Nitrogen (N) sources have been target in microalgae cultivation studies, considering their nutritional impact on growth and high costs. Here, we have evaluated the growth of Scenedesmus obliquus BR003, applying alternative low-cost culture media containing ammonium and urea, or combinations of both N sources. The culture media were applied for indoor and outdoor cultivation, followed by growth analyses and metabolic characterization. The alternative culture media B4 and L4 supported higher biomass production (1.4 g L-1) compared to BG11 (nitrate-based medium). In addition, the lipid percen...
Source: Bioprocess and Biosystems Engineering - February 4, 2020 Category: Biomedical Engineering Authors: Covell L, Machado M, Vaz MGMV, Soares J, Batista AD, Araújo WL, Martins MA, Nunes-Nesi A Tags: Bioprocess Biosyst Eng Source Type: research

Fungal biocatalysts for labdane diterpene hydroxylation.
do NA Abstract Labdane diterpenes and their derivatives have shown remarkable biological activities and are useful as chiral building blocks for the synthesis of a variety of bioactive compounds. There is great interest in developing biocatalyst technology to achieve regio- and stereoselective hydroxylation of unactivated C-H bonds in complex natural products, since the functionalization of unactivated C-H bonds generally requires hard reaction conditions and highly reactive oxidizing agents, which are limited regarding the control of regio- and stereoselectivity. Filamentous fungi are efficient biocatalysts capab...
Source: Bioprocess and Biosystems Engineering - February 4, 2020 Category: Biomedical Engineering Authors: Cruz de Carvalho T, de Oliveira Silva E, Soares GA, Parreira RLT, Ambrósio SR, Jacometti Cardoso Furtado NA Tags: Bioprocess Biosyst Eng Source Type: research

Evaluating the effects of micro-zones of granular sludge on one-stage partial nitritation-anammox nitrogen removal.
This study provides a novel perspective for understanding the effect of micro-zones of granular sludge on one-stage PN-A nitrogen removal. PMID: 32020447 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - February 4, 2020 Category: Biomedical Engineering Authors: Li H, Wang S, Zhao F, Fang F, Chen Y, Yan P, Yang J, Gu S, Guo J Tags: Bioprocess Biosyst Eng Source Type: research

Effects of different expression systems on characterization of adenylate deaminase from Aspergillus oryzae.
In this study, the effect of different hosts on the enzymatic properties of AMPD from Aspergillus oryzae GX-08 was investigated and showed that Bacillus subtilis WB600 was more suitable for producing AMPD with a higher activity of 2540 U/mL. After purification, the optimal temperature and pH of recombinant AMPD were 55 °C and pH 6.0, respectively, and its activity was significantly enhanced by 10 mM Fe3+ with an increase of 236%. More importantly, the recombinant AMPD specifically and effectively catalyzed the conversion between AMP and IMP, in which 10 mL of crude AMPD achieved a conversion ratio o...
Source: Bioprocess and Biosystems Engineering - February 4, 2020 Category: Biomedical Engineering Authors: Ke C, Li S, Cui Y, Qiu H, Pang Z Tags: Bioprocess Biosyst Eng Source Type: research

Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl2 stresses.
This study aimed to investigate the effect of a combination of two salts (NaCl/CaCl2) on lipid productivity of Chlamydomonas reinhardtii. C. reinhardtii was cultured in a two-stage culture comprising 9-day active growth in C medium followed by 3-day salt stress in C medium with various concentrations of NaCl (50‒200 mM)/CaCl2 (100 mM). In salt stress stage, NaCl (200 mM), CaCl2 (100 mM), and the NaCl/CaCl2 mixture inhibited growth but increased the lipid content in C. reinhardtii in comparison with NaCl (0, 50, and 100 mM) conditions. Combinatorial treatment with 100 mM NaCl/100 mM CaCl...
Source: Bioprocess and Biosystems Engineering - February 1, 2020 Category: Biomedical Engineering Authors: Hang LT, Mori K, Tanaka Y, Morikawa M, Toyama T Tags: Bioprocess Biosyst Eng Source Type: research

Reconstruction, verification and in-silico analysis of a genome-scale metabolic model of bacterial cellulose producing Komagataeibacter xylinus.
In this study, a comprehensive genome-scale metabolic network of Komagataeibacter xylinus as the model microorganism was reconstructed based on genome annotation, for better understanding of metabolic growth and biosynthesis of bacterial cellulose (BC). The reconstructed network included 640 genes, 783 metabolic reactions and 865 metabolites. The model was completely successful to predict the lack of growth under anaerobic conditions. Model validation by the data for the growth of acetic acid bacteria with ethanol-limited chemostat cultures showed that there is a good agreement for the O2 and CO2 fluxes with actual growth ...
Source: Bioprocess and Biosystems Engineering - February 1, 2020 Category: Biomedical Engineering Authors: Rezazadeh M, Babaeipour V, Motamedian E Tags: Bioprocess Biosyst Eng Source Type: research

Pressurized cultivation strategies for improved microbial hydrogen production by Thermococcus onnurineus NA1.
Abstract While the hydrogen economy is receiving growing attention, research on microbial hydrogen production is also increasing. Microbial water-gas shift reaction is advantageous as it produces hydrogen from by product gas including carbon monoxide (CO). However, CO solubility in water is the bottleneck of this process by low mass transfer. Thermococcus onnurineus NA1 strain can endure a high-pressure environment and can enhance hydrogen production in a pressurized reactor by increasing CO solubility. As CO causes cell toxicity, two important factors, pressure and input gas flow rate, should be considered for pr...
Source: Bioprocess and Biosystems Engineering - January 30, 2020 Category: Biomedical Engineering Authors: Kim MS, Moon M, Fitriana HN, Lee JS, Na JG, Park GW Tags: Bioprocess Biosyst Eng Source Type: research

Semi-continuous production of the anticancer drug taxol by Aspergillus fumigatus and Alternaria tenuissima immobilized in calcium alginate beads.
In this study, Aspergillus fumigatus TXD105-GM6 and Alternaria tenuissima TER995-GM3 were immobilized in calcium alginate beads and used for the production of taxol in shake flask cultures. In an effort to increase the taxol magnitude, immobilization conditions were optimized by response surface methodology program (RSM). The optimum levels of alginate concentration, calcium chloride concentration, and mycelium fresh weight were 5%, 4%, and 15% (w/v), respectively. Under these conditions, taxol production by the respective fungal strains was intensified to 901.94 μg L-1 and 529.01 μg L-1. Moreover, the...
Source: Bioprocess and Biosystems Engineering - January 30, 2020 Category: Biomedical Engineering Authors: El-Sayed ER, Ahmed AS, Hassan IA, Ismaiel AA, Karam El-Din AA Tags: Bioprocess Biosyst Eng Source Type: research

Production and characterization of bacterial cellulose obtained by Gluconacetobacter xylinus utilizing the by-products from Baijiu production.
Abstract Bacterial cellulose (BC) has extensive application prospects in many fields in view of its unique characteristics. However, the large-scale applications of BC are severely limited because of relatively low BC productivity and high cost of culture medium. Herein, the distiller's grain enzymatic hydrolysate (DEH) and yellow water were successfully combined as an effective substitute (the best distiller's grains-yellow water medium, BDY medium) for traditional Hestrin-Schramm medium (HS medium) for BC production by Gluconacetobacter xylinus through the response surface methodology. The BC production in BDY m...
Source: Bioprocess and Biosystems Engineering - January 29, 2020 Category: Biomedical Engineering Authors: He F, Yang H, Zeng L, Hu H, Hu C Tags: Bioprocess Biosyst Eng Source Type: research

Deep and high-efficiency removal of sulfate through a coupling system with sulfate-reducing and sulfur-oxidizing capacity under haloalkaliphilic condition.
This study for the first time revealed the characteristics and performance of the haloalkaliphilic CROS in deep treatment of high-sulfate wastewater, which paved the way for the development and application of this method in the real world. PMID: 31993799 [PubMed - as supplied by publisher] (Source: Bioprocess and Biosystems Engineering)
Source: Bioprocess and Biosystems Engineering - January 29, 2020 Category: Biomedical Engineering Authors: Mu T, Yang M, Xing J Tags: Bioprocess Biosyst Eng Source Type: research

Considering a membrane bioreactor for the treatment of vegetable oil refinery wastewaters at industrially relevant organic loading rates.
Abstract The present study aims to shed more light on the use of membrane bioreactors (MBRs) for the treatment of vegetable oil refinery wastewaters (VORWs). A MBR was operated for 157 days in which it was fed with real VORW of varying composition at a range of organic loading rates (0.20 ± 0.05-3.79 ± 0.29 kg COD m-3 day-1). The hitherto unconsidered fate of VORW constituents through the biological process was followed using gas chromatography/mass spectrometry analysis. This analysis revealed that only 19% of the identified feed constituents remained in th...
Source: Bioprocess and Biosystems Engineering - January 28, 2020 Category: Biomedical Engineering Authors: Abdollahzadeh Sharghi E, Shourgashti A, Bonakdarpour B Tags: Bioprocess Biosyst Eng Source Type: research

Compatible solutes adaptive alterations in Arthrobacter simplex during exposure to ethanol, and the effect of trehalose on the stress resistance and biotransformation performance.
Abstract Ethanol-tolerant Arthrobacter simplex is desirable since ethanol facilitates hydrophobic substrates dissolution on an industrial scale. Herein, alterations in compatible solutes were investigated under ethanol stress. The results showed that the amount of trehalose and glycerol increased while that of glutamate and proline decreased. The trehalose protectant role was verified and its concentration was positively related to the degree of cell tolerance. otsA, otsB and treS, three trehalose biosynthesis genes in A. simplex, also enhanced Escherichia coli stress tolerance, but the increased tolerance was dep...
Source: Bioprocess and Biosystems Engineering - January 28, 2020 Category: Biomedical Engineering Authors: Cheng HJ, Sun YH, Chang HW, Cui FF, Xue HJ, Shen YB, Wang M, Luo JM Tags: Bioprocess Biosyst Eng Source Type: research

Enhanced tolerance to inhibitors of Escherichia coli by heterologous expression of cyclopropane-fatty acid-acyl-phospholipid synthase (cfa) from Halomonas socia.
Abstract Bacteria have evolved a defense system to resist external stressors, such as heat, pH, and salt, so as to facilitate survival in changing or harsh environments. However, the specific mechanisms by which bacteria respond to such environmental changes are not completely elucidated. Here, we used halotolerant bacteria as a model to understand the mechanism conferring high tolerance to NaCl. We screened for genes related to halotolerance in Halomonas socia, which can provide guidance for practical application. Phospholipid fatty acid analysis showed that H. socia cultured under high osmotic pressure produced ...
Source: Bioprocess and Biosystems Engineering - January 27, 2020 Category: Biomedical Engineering Authors: Choi TR, Song HS, Han YH, Park YL, Park JY, Yang SY, Bhatia SK, Gurav R, Kim HJ, Lee YK, Choi KY, Yang YH Tags: Bioprocess Biosyst Eng Source Type: research

Mathematical modelling supports the existence of a threshold hydrogen concentration and media-dependent yields in the growth of a reductive acetogen.
Abstract The bacterial production of acetate via reductive acetogenesis along the Wood-Ljungdahl metabolic pathway is an important source of this molecule in several environments, ranging from industrial bioreactors to the human gastrointestinal tract. Here, we contributed to the study of reductive acetogens by considering mathematical modelling techniques for the prediction of bacterial growth and acetate production. We found that the incorporation of a hydrogen uptake concentration threshold into the models improves their predictions and we calculated this threshold as 86.2 mM (95% confidence interval 6.1-1...
Source: Bioprocess and Biosystems Engineering - January 25, 2020 Category: Biomedical Engineering Authors: Smith NW, Shorten PR, Altermann E, Roy NC, McNabb WC Tags: Bioprocess Biosyst Eng Source Type: research

Ammonium removal characteristics of heterotrophic nitrifying bacterium Pseudomonas stutzeri GEP-01 with potential for treatment of ammonium-rich wastewater.
Abstract A heterotrophic nitrifying bacterium was isolated from the activated sludge and identified as Pseudomonas stutzeri GEP-01. Strain GEP-01 exhibited an efficient heterotrophic nitrification capability and a high nitrogen utilization rate, 48 mg/L NH4+-N was removed after culturing for 24 h without NO2--N or NO3--N accumulation, and 64.7% of the NH4+-N was removed by heterotrophic nitrification. Single-factor experiments indicated that factors such as the carbon source, temperature, NH4+-N load, and inoculum size had significant effects on the ammonium removal efficiency of strain GEP-01. The prefe...
Source: Bioprocess and Biosystems Engineering - January 25, 2020 Category: Biomedical Engineering Authors: Gao J, Zhu T, Liu C, Zhang J, Gao J, Zhang J, Cai M, Li Y Tags: Bioprocess Biosyst Eng Source Type: research

O-GlcNAcylation of light chain serine 12 mediates rituximab production doubled by thiamet G.
Abstract O-Glycosylation occurs in recombinant proteins produced by CHO cells, but this phenomenon has not been studied extensively. Here, we report that rituximab is an O-linked N-acetyl-glucosaminylated (O-GlcNAcylated) protein and the production of rituximab is increased by thiamet G, an inhibitor of O-GlcNAcase. The production of rituximab doubled with OGA inhibition and decreased with O-GlcNAc transferase inhibition. O-GlcNAc-specific antibody and metabolic labelling with azidO-GlcNAc confirmed the increased O-GlcNAcylation with thiamet G. Protein mass analysis revealed that serine 7, 12, and 14 of the rituxi...
Source: Bioprocess and Biosystems Engineering - January 25, 2020 Category: Biomedical Engineering Authors: Kim HY, Park M, Kang C, Heo W, Yoon SM, Lee J, Kim JY Tags: Bioprocess Biosyst Eng Source Type: research

Characteristics of lipid biosynthesis of Chlorella pyrenoidosa under stress conditions.
Abstract Effects of high light, high salt, nitrogen and phosphorus deficiency on growth and lipid production of Chlorella pyrenoidosa were investigated in a flat-plate photoreactor, and the oil quality indexes such as CN, IV, SV, CFPP, DU, and LCSF were also evaluated. The results show that the growth of C. pyrenoidosa was inhibited under the stress conditions, but the intracellular lipid content was significantly increased. Moreover, the combustion performance, oxidation stability, low temperature fluidity, and other oil quality indicators under these nutrient stress conditions were significantly improved. Import...
Source: Bioprocess and Biosystems Engineering - January 18, 2020 Category: Biomedical Engineering Authors: Dong L, Li D, Li C Tags: Bioprocess Biosyst Eng Source Type: research