A dual fluorescent herpes simplex virus type 1 recombinant reveals divergent outcomes of neuronal infection
We report that the transcriptional state of neuronal HSV-1 infection is variable in response to changes in the intracellular neuronal environment.IMPORTANCEHerpes simplex virus type 1 (HSV-1) is a prevalent human pathogen that infects approximately 67% of the global human population. HSV-1 invades the peripheral nervous system, where latent HSV-1 infection persists within the host for life. Immunological evasion, viral persistence, and herpetic pathologies are determined by the regulation of HSV-1 gene expression. Studying HSV-1 gene expression during neuronal infection is challenging but essential for the development of a...
Source: Herpes - April 23, 2024 Category: Infectious Diseases Authors: Luke F Domanico Gary P Dunn Oren Kobiler Matthew P Taylor Source Type: research
A dual fluorescent herpes simplex virus type 1 recombinant reveals divergent outcomes of neuronal infection
We report that the transcriptional state of neuronal HSV-1 infection is variable in response to changes in the intracellular neuronal environment.IMPORTANCEHerpes simplex virus type 1 (HSV-1) is a prevalent human pathogen that infects approximately 67% of the global human population. HSV-1 invades the peripheral nervous system, where latent HSV-1 infection persists within the host for life. Immunological evasion, viral persistence, and herpetic pathologies are determined by the regulation of HSV-1 gene expression. Studying HSV-1 gene expression during neuronal infection is challenging but essential for the development of a...
Source: Herpes - April 23, 2024 Category: Infectious Diseases Authors: Luke F Domanico Gary P Dunn Oren Kobiler Matthew P Taylor Source Type: research
ISGylation by HERCs facilitates STING activation
Cell Rep. 2024 Apr 21;43(5):114135. doi: 10.1016/j.celrep.2024.114135. Online ahead of print.ABSTRACTOptimal activation of stimulator of interferon genes (STING) protein is crucial for host defenses against pathogens and avoiding detrimental effects. Various post-translational modifications control STING activity. However, the function of interferon (IFN)-stimulated gene (ISG) 15 modification (ISGylation) in controlling STING stability and activation is unclear. Here, we show that the E3 ISGylation ligases HECT domain- and RCC1-like domain-containing proteins (HERCs; HERC5 in humans and HERC6 in mice) facilitate STING acti...
Source: Herpes - April 23, 2024 Category: Infectious Diseases Authors: Ying Qin Min Wang Xintong Meng Mengge Wang Haojia Jiang Yanjie Gao Jingxin Li Chunyuan Zhao Chaofeng Han Wei Zhao Xuexing Zheng Source Type: research
HSV-1 employs UL56 to antagonize expression and function of cGAMP channels
We report that infection with multiple human viruses depletes some of these cGAMP conduits. This includes herpes simplex virus 1 (HSV-1) that targets SLC46A2, P2X7, and the VRAC subunits LRRC8A and LRRC8C for degradation. The HSV-1 protein UL56 is necessary and sufficient for these effects that are mediated at least partially by proteasomal turnover. UL56 thereby inhibits cGAMP uptake via VRAC, SLC46A2, and P2X7. Taken together, HSV-1 antagonizes intercellular cGAMP transfer. We propose that this limits innate immunity by reducing cell-to-cell communication via the immunotransmitter cGAMP.PMID:38652659 | DOI:10.1016/j.celr...
Source: Herpes - April 23, 2024 Category: Infectious Diseases Authors: Henry T W Blest Alexander Redmond Jed Avissar Jake Barker Anne Bridgeman Gerissa Fowler Lise Chauveau Jonny Hertzog Iolanda Vendrell Roman Fischer Marie B Iversen Lichen Jing David M Koelle S øren R Paludan Benedikt M Kessler Colin M Crump Jan Rehwinkel Source Type: research
Microbial keratitis in Southern Malawi: a microbiological pilot study
CONCLUSIONS: In a resource-poor setting, a CIM can be used to safely sample the cornea in patients presenting with suspected MK, enabling identification of causative microorganisms by culture and PCR. Although the microbiological spectrum found was limited to the dry season, these preliminary results could be used to guide empirical treatment.PMID:38653537 | DOI:10.1136/bmjophth-2024-001682 (Source: Herpes)
Source: Herpes - April 23, 2024 Category: Infectious Diseases Authors: Tobi F Somerville Shaffi Mdala Thokozani Zungu Moira Gandiwa Rose Herbert Dean Everett Caroline E Corless Nicholas A V Beare Timothy Neal Malcolm J Horsburgh Alistair Darby Stephen B Kaye Petros C Kayange Source Type: research
Analysis of weighted gene co-expression networks and clinical validation identify hub genes and immune cell infiltration in the endometrial cells of patients with recurrent implantation failure
CONCLUSION: We first utilized WGCNA to identify the most potential nine hub genes which might be associated with RIF. Meanwhile, this study offers insights into the landscape of immune infiltration status to reveal the underlying immune pathogenesis of RIF. This may be a direction for the next study of RIF etiology. Further studies would be required to investigate the involved mechanisms.PMID:38645487 | PMC:PMC11026622 | DOI:10.3389/fgene.2024.1292757 (Source: Herpes)
Source: Herpes - April 22, 2024 Category: Infectious Diseases Authors: Zhenteng Liu Shoucui Lai Qinglan Qu Xuemei Liu Wei Zhang Dongmei Zhao Shunzhi He Yuxia Sun Hongchu Bao Source Type: research
Epstein-Barr virus, Cytomegalovirus, and Herpes Simplex-1/2 reactivations in critically ill patients with COVID-19
CONCLUSIONS: Human Herpesviruses reactivations in critically ill patients with COVID-19 severe Pneumonia are associated with mortality and with a higher risk to develop both VAP and ICU-BSI.PMID:38649512 | DOI:10.1186/s40635-024-00624-9 (Source: Herpes)
Source: Herpes - April 22, 2024 Category: Infectious Diseases Authors: Alessia Mattei Lorenzo Schiavoni Elisabetta Riva Massimo Ciccozzi Roberta Veralli Angela Urselli Vincenzo Citriniti Antonio Nenna Giuseppe Pascarella Fabio Costa Rita Cataldo Felice Eugenio Agr ò Massimiliano Carassiti Source Type: research
Shared genetic aetiology of Alzheimer's disease and age-related macular degeneration by APOC1 and APOE genes
CONCLUSION: In this study, we confirmed the genetic pleiotropy between AD and AMD and identified APOC1 and APOE as pleiotropic genes. Further, the integration of multiomics data identified ZNF131, ADNP2 and HINFP as novel diagnostic biomarkers for AD and AMD.PMID:38646507 | PMC:PMC11029327 | DOI:10.1136/bmjno-2023-000570 (Source: Herpes)
Source: Herpes - April 22, 2024 Category: Infectious Diseases Authors: Xueli Zhang Zhuoting Zhu Yu Huang Xianwen Shang Terence J O'Brien Patrick Kwan Jason Ha Wei Wang Shunming Liu Xiayin Zhang Katerina Kiburg Yining Bao Jing Wang Honghua Yu Mingguang He Lei Zhang Source Type: research
Epstein –Barr virus, Cytomegalovirus, and Herpes Simplex-1/2 reactivations in critically ill patients with COVID-19
To assess the incidences of Herpes Simplex-1 and 2 (HSV-1, HSV-2), Cytomegalovirus (CMV), Epstein –Barr Virus (EBV) reactivations in critically ill COVID-19 patients. To determine the association between viral ... (Source: Intensive Care Medicine Experimental)
Source: Intensive Care Medicine Experimental - April 22, 2024 Category: Surgery Authors: Alessia Mattei, Lorenzo Schiavoni, Elisabetta Riva, Massimo Ciccozzi, Roberta Veralli, Angela Urselli, Vincenzo Citriniti, Antonio Nenna, Giuseppe Pascarella, Fabio Costa, Rita Cataldo, Felice Eugenio Agr ò and Massimiliano Carassiti Tags: Research Articles Source Type: research
Characterization and optimization of exopolysaccharide extracted from a newly isolated halotolerant cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1 with antiviral activity
This study aimed to identify and optimize the conditions for exopolysaccharide (EPS) production from a newly isolated cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1, besides exploring its antiviral activity. The cyanobacterial EPS was purified through DEAE-52 cellulose column with a final yield of 83.75%. Different analysis instruments were applied for EPS identification, including Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), and gas chromatographic-mass spectrometry (GC-MS). Plackett-Burman's design demonstrated that working volume (X1), EDTA (X2), inoculum size (X3), CaCl2 (X4...
Source: Herpes - April 21, 2024 Category: Infectious Diseases Authors: Mabroka H Saad Nagwa M Sidkey Esmail M El-Fakharany Source Type: research
Association of Herpes simplex infection with significantly increased risk of head and neck cancer: real-world evidence of about 500,000 patients
CONCLUSION: The surprisingly strong correlation with lip, oral cavity, and pharynx neoplasms sheds new light on supposedly harmless herpes simplex infections, suggesting them as a possible new factor for risk stratification.PMID:38643367 | DOI:10.1111/ijd.17196 (Source: Herpes)
Source: Herpes - April 21, 2024 Category: Infectious Diseases Authors: Jennifer von Stebut Max Heiland Robert Preissner Carsten Rendenbach Saskia Preissner Source Type: research
Replacement of sulfonamide by sulfoximine within a helicase-primase inhibitor with restricted flexibility
Bioorg Med Chem Lett. 2024 Apr 18:129761. doi: 10.1016/j.bmcl.2024.129761. Online ahead of print.ABSTRACTHelicase-primase is an interesting target for the therapy of herpes simplex virus (HSV) infections. Since amenamevir is already approved for varicella-zoster virus (VZV) and HSV in Japan and pritelivir has received breakthrough therapy status for the treatment of acyclovir-resistant HSV infections in immunocompromised patients, the target has sparked interest in me-too approaches. Here, we describe the attempt to improve nervous tissue penetration in Phaeno Therapeutics drug candidate HN0037 to target the latent reservo...
Source: Herpes - April 20, 2024 Category: Infectious Diseases Authors: Christian Gege Gerald Kleymann Source Type: research
Replacement of sulfonamide by sulfoximine within a helicase-primase inhibitor with restricted flexibility
Bioorg Med Chem Lett. 2024 Apr 18:129761. doi: 10.1016/j.bmcl.2024.129761. Online ahead of print.ABSTRACTHelicase-primase is an interesting target for the therapy of herpes simplex virus (HSV) infections. Since amenamevir is already approved for varicella-zoster virus (VZV) and HSV in Japan and pritelivir has received breakthrough therapy status for the treatment of acyclovir-resistant HSV infections in immunocompromised patients, the target has sparked interest in me-too approaches. Here, we describe the attempt to improve nervous tissue penetration in Phaeno Therapeutics drug candidate HN0037 to target the latent reservo...
Source: Bioorganic and Medicinal Chemistry Letters - April 20, 2024 Category: Chemistry Authors: Christian Gege Gerald Kleymann Source Type: research
Oncolytic virotherapy stimulates anti ‑tumor immune response and demonstrates activity in advanced sarcoma: Report of two cases
Oncol Lett. 2024 Apr 3;27(6):244. doi: 10.3892/ol.2024.14377. eCollection 2024 Jun.ABSTRACTSarcoma is derived from mesenchymal neoplasms and has numerous subtypes, accounting for 1% of all adult malignancies and 15% of childhood malignancies. The prognosis of metastatic or recurrent sarcoma remains poor. The current study presents two cases of sarcoma enrolled in a phase I dose escalation trial for solid tumor, who had previously failed all standard therapies. These patients were treated with VG161, an immune-stimulating herpes simplex virus type 1 oncolytic virus with payloads of IL-12, IL-15 and IL-15 receptor α unit, a...
Source: Oncology Letters - April 19, 2024 Category: Cancer & Oncology Authors: Yeting Qiu Aijun Qin Ronghua Zhao Jun Ding William Wei-Guo Jia Manu Singh Yanal Murad Qian Tan Ganessan Kichenadasse Source Type: research
Oncolytic virotherapy stimulates anti ‑tumor immune response and demonstrates activity in advanced sarcoma: Report of two cases
Oncol Lett. 2024 Apr 3;27(6):244. doi: 10.3892/ol.2024.14377. eCollection 2024 Jun.ABSTRACTSarcoma is derived from mesenchymal neoplasms and has numerous subtypes, accounting for 1% of all adult malignancies and 15% of childhood malignancies. The prognosis of metastatic or recurrent sarcoma remains poor. The current study presents two cases of sarcoma enrolled in a phase I dose escalation trial for solid tumor, who had previously failed all standard therapies. These patients were treated with VG161, an immune-stimulating herpes simplex virus type 1 oncolytic virus with payloads of IL-12, IL-15 and IL-15 receptor α unit, a...
Source: Herpes - April 19, 2024 Category: Infectious Diseases Authors: Yeting Qiu Aijun Qin Ronghua Zhao Jun Ding William Wei-Guo Jia Manu Singh Yanal Murad Qian Tan Ganessan Kichenadasse Source Type: research
