Non-Thermal Plasma Reduces HSV-1 Infection of and Replication in HaCaT Keratinocytes In Vitro
Int J Mol Sci. 2024 Mar 29;25(7):3839. doi: 10.3390/ijms25073839.ABSTRACTHerpes simplex virus type 1 (HSV-1) is a lifelong pathogen characterized by asymptomatic latent infection in the trigeminal ganglia (TG), with periodic outbreaks of cold sores caused by virus reactivation in the TG and subsequent replication in the oral mucosa. While antiviral therapies can provide relief from cold sores, they are unable to eliminate HSV-1. We provide experimental results that highlight non-thermal plasma (NTP) as a new alternative therapy for HSV-1 infection that would resolve cold sores faster and reduce the establishment of latent ...
Source: Herpes - April 13, 2024 Category: Infectious Diseases Authors: Julia Sutter Jascha Brettschneider Brian Wigdahl Peter J Bruggeman Fred C Krebs Vandana Miller Source Type: research
Behavioral toxicity and neurotoxic mechanisms of PLA-PBAT biodegradable microplastics in zebrafish
This study searched to investigate the acute and sub-chronic toxicity of polylactic acid (PLA) and polybutyleneadipate-co-terephthalate (PLA-PBAT) bio-microplastics on 3-month-old zebrafish to elucidate their potential toxic mechanisms. Acute toxicity assessments revealed 96 h-LC50 value of 12.69 mg/L for PLA-PBAT. Sub-chronic exposure of over 21 days revealed deviations in critical behavioral patterns and physiological indicators. In treated groups, weight gain and specific growth rates were significantly lower than those obtained for the control group, such that high doses induced significant reductions in total organ co...
Source: Herpes - April 13, 2024 Category: Infectious Diseases Authors: Deyong Zhang Xiaolu Xu Yin Lu Liang Guo Source Type: research
Non-Thermal Plasma Reduces HSV-1 Infection of and Replication in HaCaT Keratinocytes In Vitro
Int J Mol Sci. 2024 Mar 29;25(7):3839. doi: 10.3390/ijms25073839.ABSTRACTHerpes simplex virus type 1 (HSV-1) is a lifelong pathogen characterized by asymptomatic latent infection in the trigeminal ganglia (TG), with periodic outbreaks of cold sores caused by virus reactivation in the TG and subsequent replication in the oral mucosa. While antiviral therapies can provide relief from cold sores, they are unable to eliminate HSV-1. We provide experimental results that highlight non-thermal plasma (NTP) as a new alternative therapy for HSV-1 infection that would resolve cold sores faster and reduce the establishment of latent ...
Source: Herpes - April 13, 2024 Category: Infectious Diseases Authors: Julia Sutter Jascha Brettschneider Brian Wigdahl Peter J Bruggeman Fred C Krebs Vandana Miller Source Type: research
Behavioral toxicity and neurotoxic mechanisms of PLA-PBAT biodegradable microplastics in zebrafish
This study searched to investigate the acute and sub-chronic toxicity of polylactic acid (PLA) and polybutyleneadipate-co-terephthalate (PLA-PBAT) bio-microplastics on 3-month-old zebrafish to elucidate their potential toxic mechanisms. Acute toxicity assessments revealed 96 h-LC50 value of 12.69 mg/L for PLA-PBAT. Sub-chronic exposure of over 21 days revealed deviations in critical behavioral patterns and physiological indicators. In treated groups, weight gain and specific growth rates were significantly lower than those obtained for the control group, such that high doses induced significant reductions in total organ co...
Source: Herpes - April 13, 2024 Category: Infectious Diseases Authors: Deyong Zhang Xiaolu Xu Yin Lu Liang Guo Source Type: research
A putative design for the electromagnetic activation of split proteins for molecular and cellular manipulation
Front Bioeng Biotechnol. 2024 Mar 28;12:1355915. doi: 10.3389/fbioe.2024.1355915. eCollection 2024.ABSTRACTThe ability to manipulate cellular function using an external stimulus is a powerful strategy for studying complex biological phenomena. One approach to modulate the function of the cellular environment is split proteins. In this method, a biologically active protein or an enzyme is fragmented so that it reassembles only upon a specific stimulus. Although many tools are available to induce these systems, nature has provided other mechanisms to expand the split protein toolbox. Here, we show a novel method for reconsti...
Source: Herpes - April 12, 2024 Category: Infectious Diseases Authors: Connor J Grady E Alejandro Castellanos Franco Jory Schossau Ryan C Ashbaugh Galit Pelled Assaf A Gilad Source Type: research
A putative design for the electromagnetic activation of split proteins for molecular and cellular manipulation
Front Bioeng Biotechnol. 2024 Mar 28;12:1355915. doi: 10.3389/fbioe.2024.1355915. eCollection 2024.ABSTRACTThe ability to manipulate cellular function using an external stimulus is a powerful strategy for studying complex biological phenomena. One approach to modulate the function of the cellular environment is split proteins. In this method, a biologically active protein or an enzyme is fragmented so that it reassembles only upon a specific stimulus. Although many tools are available to induce these systems, nature has provided other mechanisms to expand the split protein toolbox. Here, we show a novel method for reconsti...
Source: Herpes - April 12, 2024 Category: Infectious Diseases Authors: Connor J Grady E Alejandro Castellanos Franco Jory Schossau Ryan C Ashbaugh Galit Pelled Assaf A Gilad Source Type: research
Amenamevir for Treating Acyclovir-Resistant or Refractory Herpes Simplex Virus Infection in Allogeneic Hematopoietic Stem Cell Transplantation Recipients. Two Case Reports
Int J Antimicrob Agents. 2024 Apr 10:107169. doi: 10.1016/j.ijantimicag.2024.107169. Online ahead of print.NO ABSTRACTPMID:38608846 | DOI:10.1016/j.ijantimicag.2024.107169 (Source: Herpes)
Source: Herpes - April 12, 2024 Category: Infectious Diseases Authors: L éo Sallée Laetitia Souchet David Boutolleau St éphanie Nguyen Source Type: research
Improving polymerase chain reaction diagnostic rates for herpes simplex keratitis: results of a pilot study
CONCLUSION: Our results suggest that, with proper swabbing technique, PCR may be more sensitive than previously reported.PMID:38601900 | PMC:PMC11001569 | DOI:10.5693/djo.01.2024.01.002 (Source: Herpes)
Source: Herpes - April 11, 2024 Category: Infectious Diseases Authors: Alice L Milligan Jeremy J Hoffman Yan Ning Neo Su-Yin Koay Source Type: research
Emerging drugs for the treatment of herpetic keratitis
Expert Opin Emerg Drugs. 2024 Apr 11:1-14. doi: 10.1080/14728214.2024.2339899. Online ahead of print.ABSTRACTINTRODUCTION: Herpes simplex keratitis stands as a prominent factor contributing to infectious blindness among developed nations. On a global scale, over 60% of the population tests positive for herpes simplex virus type-1 (HSV-1). Despite these statistics, there is currently no vaccine available for the virus. Moreover, the conventional nucleoside drugs prescribed to patients are proving ineffective in addressing issues related to drug resistance, recurrence, latency, and the escalating risk of vision loss. Hence, ...
Source: Expert Opinion on Emerging Drugs - April 11, 2024 Category: Drugs & Pharmacology Authors: Divya Kapoor Pankaj Sharma Deepak Shukla Source Type: research
Emerging drugs for the treatment of herpetic keratitis
Expert Opin Emerg Drugs. 2024 Apr 11:1-14. doi: 10.1080/14728214.2024.2339899. Online ahead of print.ABSTRACTINTRODUCTION: Herpes simplex keratitis stands as a prominent factor contributing to infectious blindness among developed nations. On a global scale, over 60% of the population tests positive for herpes simplex virus type-1 (HSV-1). Despite these statistics, there is currently no vaccine available for the virus. Moreover, the conventional nucleoside drugs prescribed to patients are proving ineffective in addressing issues related to drug resistance, recurrence, latency, and the escalating risk of vision loss. Hence, ...
Source: Herpes - April 11, 2024 Category: Infectious Diseases Authors: Divya Kapoor Pankaj Sharma Deepak Shukla Source Type: research
Improving polymerase chain reaction diagnostic rates for herpes simplex keratitis: results of a pilot study
CONCLUSION: Our results suggest that, with proper swabbing technique, PCR may be more sensitive than previously reported.PMID:38601900 | PMC:PMC11001569 | DOI:10.5693/djo.01.2024.01.002 (Source: Herpes)
Source: Herpes - April 11, 2024 Category: Infectious Diseases Authors: Alice L Milligan Jeremy J Hoffman Yan Ning Neo Su-Yin Koay Source Type: research
Emerging drugs for the treatment of herpetic keratitis
Expert Opin Emerg Drugs. 2024 Apr 11:1-14. doi: 10.1080/14728214.2024.2339899. Online ahead of print.ABSTRACTINTRODUCTION: Herpes simplex keratitis stands as a prominent factor contributing to infectious blindness among developed nations. On a global scale, over 60% of the population tests positive for herpes simplex virus type-1 (HSV-1). Despite these statistics, there is currently no vaccine available for the virus. Moreover, the conventional nucleoside drugs prescribed to patients are proving ineffective in addressing issues related to drug resistance, recurrence, latency, and the escalating risk of vision loss. Hence, ...
Source: Expert Opinion on Emerging Drugs - April 11, 2024 Category: Drugs & Pharmacology Authors: Divya Kapoor Pankaj Sharma Deepak Shukla Source Type: research
Left dural carotid cavernous fistula mimicking an inflammatory process: A case report
Radiol Case Rep. 2024 Apr 5;19(6):2558-2560. doi: 10.1016/j.radcr.2024.03.021. eCollection 2024 Jun.ABSTRACTA female in her 70s presented with altered mental status, left eye pain, ophthalmalgia, and diplopia following a fall. Brain MRI demonstrated contrast-enhancing left peri-insular T2 hyperintense changes that was read as possible herpes simplex encephalitis by neuroradiology. Cerebral angiogram revealed a Barrow Type D left sided carotid cavernous fistula. The patient was subsequently treated with endovascular transvenous coil embolization of the left cavernous sinus resulting in complete occlusion of the fistula. The...
Source: Herpes - April 10, 2024 Category: Infectious Diseases Authors: Annika K Oie Amanda A Herrmann Michael H Rosenbloom Source Type: research
TheraVision: Engineering platform technology for the development of oncolytic viruses based on herpes simplex virus type 1
Mol Ther Oncol. 2024 Feb 28;32(1):200784. doi: 10.1016/j.omton.2024.200784. eCollection 2024 Mar 21.ABSTRACTViruses are able to efficiently penetrate cells, multiply, and eventually kill infected cells, release tumor antigens, and activate the immune system. Therefore, viruses are highly attractive novel agents for cancer therapy. Clinical trials with first generations of oncolytic viruses (OVs) are very promising but show significant need for optimization. The aim of TheraVision was to establish a broadly applicable engineering platform technology for combinatorial oncolytic virus and immunotherapy. Through genetic engine...
Source: Herpes - April 10, 2024 Category: Infectious Diseases Authors: Christina Funk Nadja Uhlig Zsolt Ruzsics Florentin Baur Matthias Peindl Sarah Nietzer Karina Epting Gabriele Vacun Gudrun Dandekar Catherine Botteron Christian Werno Thomas Grunwald Susanne M Bailer Source Type: research
Oncolytic herpes simplex viruses designed for targeted treatment of EGFR-bearing tumors
Mol Ther Oncol. 2024 Jan 10;32(1):200761. doi: 10.1016/j.omton.2024.200761. eCollection 2024 Mar 21.ABSTRACTOncolytic herpes simplex viruses (oHSVs) have emerged as leading cancer therapeutic agents. Effective oHSV virotherapy may ultimately require both intratumoral and systemic vector administration to target the primary tumor and distant metastases. An attractive approach to enhancing oHSV tumor specificity is engineering the virus envelope glycoproteins for selective recognition of and infection via tumor-specific cell surface proteins. We previously demonstrated that oHSVs could be retargeted to EGFR-expressing cells ...
Source: Herpes - April 10, 2024 Category: Infectious Diseases Authors: Selene Ingusci Bonnie L Hall Justus B Cohen Joseph C Glorioso Source Type: research
