PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection
J Med Virol. 2024 Mar;96(3):e29538. doi: 10.1002/jmv.29538.ABSTRACTTo compare prevalence of positive PCR tests for herpesviruses between patients with and without a history of clinical corneal endothelial allograft rejection (AGR). Retrospective cross-sectional study with two-group comparison. A total of 307 aqueous humor (AH) samples from 235 Patients and 244 eyes who underwent penetrating keratoplasty or Descemet membrane endothelial keratoplasty or had a diagnostic AH aspiration due to clinical AGR between 2019 and 2023 were tested for DNA of herpes simplex virus (HSV), varicella-zoster virus (VZV), cytomegalovirus (CMV...
Source: Herpes - March 20, 2024 Category: Infectious Diseases Authors: Yaser Abu Dail Loay Daas Elias Flockerzi Cristian Munteanu Julian Kahlert Sigrun Smola Berthold Seitz Source Type: research
PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection
J Med Virol. 2024 Mar;96(3):e29538. doi: 10.1002/jmv.29538.ABSTRACTTo compare prevalence of positive PCR tests for herpesviruses between patients with and without a history of clinical corneal endothelial allograft rejection (AGR). Retrospective cross-sectional study with two-group comparison. A total of 307 aqueous humor (AH) samples from 235 Patients and 244 eyes who underwent penetrating keratoplasty or Descemet membrane endothelial keratoplasty or had a diagnostic AH aspiration due to clinical AGR between 2019 and 2023 were tested for DNA of herpes simplex virus (HSV), varicella-zoster virus (VZV), cytomegalovirus (CMV...
Source: Herpes - March 20, 2024 Category: Infectious Diseases Authors: Yaser Abu Dail Loay Daas Elias Flockerzi Cristian Munteanu Julian Kahlert Sigrun Smola Berthold Seitz Source Type: research
Therapeutic targeting at genome mutations of liver cancer by the insertion of HSV1 thymidine kinase through Cas9-mediated editing
CONCLUSIONS: Our studies suggested that mutation targeting may hold promise as a versatile and effective approach to treating liver cancers.PMID:38497929 | PMC:PMC10948134 | DOI:10.1097/HC9.0000000000000412 (Source: Cancer Control)
Source: Cancer Control - March 18, 2024 Category: Cancer & Oncology Authors: Muhamuda Kader Wei Sun Bao-Guo Ren Yan-Ping Yu Junyan Tao Lesley M Foley Silvia Liu Satdarshan P Monga Jian-Hua Luo Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Herpes - March 18, 2024 Category: Infectious Diseases Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
Therapeutic targeting at genome mutations of liver cancer by the insertion of HSV1 thymidine kinase through Cas9-mediated editing
CONCLUSIONS: Our studies suggested that mutation targeting may hold promise as a versatile and effective approach to treating liver cancers.PMID:38497929 | PMC:PMC10948134 | DOI:10.1097/HC9.0000000000000412 (Source: Herpes)
Source: Herpes - March 18, 2024 Category: Infectious Diseases Authors: Muhamuda Kader Wei Sun Bao-Guo Ren Yan-Ping Yu Junyan Tao Lesley M Foley Silvia Liu Satdarshan P Monga Jian-Hua Luo Source Type: research
The Chinese herbal prescription JZ-1 promotes extracellular vesicle production and protects against herpes simplex virus type 2 infection in vitro
CONCLUSION: JZ-1 had an anti-HSV-2 efficacy. After JZ-1 stimulation, VK2/E6E7 cells secreted extracellular vesicles which protect host cells from HSV-2 infection. High-throughput sequencing showed that these extracellular vesicles contained a large number of miRNAs targeting PI3K/AKT/mTOR pathway. JZ-1 group extracellular vesicles could inhibit the activation of AKT/mTOR pathway and induce the host cells autophagy.PMID:38495169 | PMC:PMC10940933 | DOI:10.1016/j.heliyon.2024.e27019 (Source: Herpes)
Source: Herpes - March 18, 2024 Category: Infectious Diseases Authors: Wenjia Wang Ximing Jin Qingqing Shao Tong Liu Tianli Liu Xinwei Zhao Lijun Xu Wen Gao Liu Hu Zhuo Chen Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
The Chinese herbal prescription JZ-1 promotes extracellular vesicle production and protects against herpes simplex virus type 2 infection in vitro
CONCLUSION: JZ-1 had an anti-HSV-2 efficacy. After JZ-1 stimulation, VK2/E6E7 cells secreted extracellular vesicles which protect host cells from HSV-2 infection. High-throughput sequencing showed that these extracellular vesicles contained a large number of miRNAs targeting PI3K/AKT/mTOR pathway. JZ-1 group extracellular vesicles could inhibit the activation of AKT/mTOR pathway and induce the host cells autophagy.PMID:38495169 | PMC:PMC10940933 | DOI:10.1016/j.heliyon.2024.e27019 (Source: Herpes)
Source: Herpes - March 18, 2024 Category: Infectious Diseases Authors: Wenjia Wang Ximing Jin Qingqing Shao Tong Liu Tianli Liu Xinwei Zhao Lijun Xu Wen Gao Liu Hu Zhuo Chen Source Type: research
Therapeutic targeting at genome mutations of liver cancer by the insertion of HSV1 thymidine kinase through Cas9-mediated editing
CONCLUSIONS: Our studies suggested that mutation targeting may hold promise as a versatile and effective approach to treating liver cancers.PMID:38497929 | PMC:PMC10948134 | DOI:10.1097/HC9.0000000000000412 (Source: Herpes)
Source: Herpes - March 18, 2024 Category: Infectious Diseases Authors: Muhamuda Kader Wei Sun Bao-Guo Ren Yan-Ping Yu Junyan Tao Lesley M Foley Silvia Liu Satdarshan P Monga Jian-Hua Luo Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Herpes - March 18, 2024 Category: Infectious Diseases Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ra...
Source: Analytica Chimica Acta - March 18, 2024 Category: Chemistry Authors: Liming Zhang Jieyu Liu Shisi Huang Wentao Zeng Li Li Xihao Fan Zhuoxuan Lu Source Type: research
GSE254432 CCR2 and CCR7 promote inflammatory monocyte recruitment through synergistic roles during encephalitic virus infection, but the monocyte response in the brain is dependent on the infecting virus
Contributors : Clayton W Winkler ; Alyssa B Evans ; Aaron B Carmody ; Justin B Lack ; Tyson A Woods ; Karin E PetersonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusInflammatory monocytes (iMO) migrate from the bone marrow to the brain during viral encephalitis. For many viruses, including Herpes simplex virus type-1 (HSV), iMO recruitment is dependent on the chemokine receptor CCR2. However, La Crosse virus (LACV) induces iMO recruitment independent of CCR2. Comparison of iMOs from HSV and LACV-infected mice showed higher expression of the g protein-coupled receptor CCR7 in LACV-ind...
Source: GEO: Gene Expression Omnibus - March 18, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Movement disorders associated with pediatric encephalitis
Handb Clin Neurol. 2024;200:229-238. doi: 10.1016/B978-0-12-823912-4.00018-9.ABSTRACTNew onset movement disorders are a common clinical problem in pediatric neurology and can be infectious, inflammatory, metabolic, or functional in origin. Encephalitis is one of the more important causes of new onset movement disorders, and movement disorders are a common feature (~25%) of all encephalitis. However, all encephalitides are not the same, and movement disorders are a key diagnostic feature that can help the clinician identify the etiology of the encephalitis, and therefore appropriate treatment is required. Movement disorders...
Source: Herpes - March 17, 2024 Category: Infectious Diseases Authors: Russell C Dale Shekeeb S Mohammad Source Type: research
