Effects of N-Acetylcysteine and N-Acetylcysteine Amide on Erythrocyte Deformability and Oxidative Stress in a Rat Model of Lower Extremity Ischemia-Reperfusion Injury.
Conclusion. The results of our study demonstrated the protective effects of NAC and NACA on erythrocyte deformability and oxidative damage in skeletal muscle in lower extremity ischemia-reperfusion. NAC and NACA exhibited similar protective effects on oxidative damage and erythrocyte deformability.
PMID: 33062321 [PubMed] (Source: Cardiology Research and Practice)
Source: Cardiology Research and Practice - October 21, 2020 Category: Cardiology Authors: Erol G, Kartal H, Comu FM, Cetin E, Demirdas E, Sicim H, Unal CS, Gunay C, Oz BS, Bolcal C Tags: Cardiol Res Pract Source Type: research
N-acetylcysteine and glycyrrhizin combination: Benefit outcome in a murine model of acetaminophen-induced liver failure.
CONCLUSION: We demonstrate that, compared to N-acetylcysteine alone, co-administration of glycyrrhizin decreases the liver necrosis score and improves survival in a murine model of acetaminophen-induced liver injury. Our study opens a potential new therapeutic pathway in the prevention of acetaminophen hepatotoxicity.
PMID: 33033567 [PubMed] (Source: World Journal of Hepatology)
Source: World Journal of Hepatology - October 10, 2020 Category: Gastroenterology Tags: World J Hepatol Source Type: research
N-acetylcysteine alleviates liver injury by suppressing macrophage-mediated inflammatory response post microwave ablation.
CONCLUSION: Our results demonstrated that N-acetyl-cysteine application alleviate macrophages aggregation and inflammatory response in liver suffering microwave ablation, and mitigating liver injury and cell apoptosis.
PMID: 32438077 [PubMed - as supplied by publisher] (Source: International Immunopharmacology)
Source: International Immunopharmacology - May 17, 2020 Category: Allergy & Immunology Authors: Zhou H, Sun Y, Wang Q, Li Z, Zhong W, Wang X, Dai X, Kong L Tags: Int Immunopharmacol Source Type: research
Diabetes aggravates myocardial ischaemia reperfusion injury via activating Nox2-related programmed cell death in an AMPK-dependent manner.
This study was designed to expose the crosstalk between oxidative stress and AMPK, a vital molecule that controls biological energy metabolism, in myocardial ischaemia reperfusion injury (I/RI) in diabetic rats. Diabetes was stimulated in rats using streptozotocin injection. Rats were separated on random into control, control + I/R, Diabetes, Diabetes + I/R, Diabetes + I/R + N-acetylcysteine and Diabetes + I/R + Vas2870 groups. Myocardial infarct size was determined, and the predominant Nox family isoforms were analysed. In vitro, the H9C2 cells were administered excess glucose and exposed to hypoxia/reoxygenat...
Source: J Cell Mol Med - April 28, 2020 Category: Molecular Biology Authors: Wang C, Zhu L, Yuan W, Sun L, Xia Z, Zhang Z, Yao W Tags: J Cell Mol Med Source Type: research
Intratympanic application of poloxamer 407 hydrogels results in sustained N-acetylcysteine delivery to the inner ear.
This study focused on (i) the evaluation of concentration-time profiles of N-acetylcysteine in perilymph, cerebrospinal fluid and plasma after intratympanic administration, (ii) the influence of the dosage form, i.e. a thermoreversible poloxamer 407 hydrogel versus a solution, on N-acetylcysteine pharmacokinetics, and (iii) the development of a pH- and osmolality-adjusted formulation for intratympanic N-acetylcysteine delivery. 49 female albino guinea pigs were randomized into two treatment groups, receiving either a single intratympanic injection of a 4% N-acetylcysteine poloxamer 407 hydrogel or a 4% N-acetylcysteine sol...
Source: European Journal of Pharmaceutics and Biopharmaceutics - March 11, 2020 Category: Drugs & Pharmacology Authors: Clara Gausterer J, Saidov N, Ahmadi N, Zhu C, Wirth M, Reznicek G, Arnoldner C, Gabor F, Honeder C Tags: Eur J Pharm Biopharm Source Type: research
Alternatively activated macrophages promote resolution of necrosis following acute liver injury
After an overdose of acetaminophen (paracetamol), some patients present to hospital too late for the current antidote (n-acetylcysteine) to be effective. We tested whether macrophages, an injury-responsive leukocyte that can scavenge dead/dying cells, could serve as a cell-based therapy in an experimental model of acetaminophen overdose. Injection of alternatively-activated macrophages rapidly reduced liver injury and reduced several mediators of inflammation. Macrophages show promise to serve as a potential cell-based therapy for acute liver injury. (Source: Journal of Hepatology)
Source: Journal of Hepatology - March 10, 2020 Category: Gastroenterology Authors: Philip Starkey Lewis, Lara Campana, Niya Aleksieva, Jennifer Ann Cartwright, Alison Mackinnon, Eoghan O ’Duibhir, Timothy Kendall, Matthieu Vermeren, Adrian Thomson, Victoria Gadd, Benjamin Dwyer, Rhona Aird, Tak-Yung Man, Adriano Giorgio Rossi, Lesley Source Type: research
Protective effect of N-acetyl cysteine against radiotherapy-induced cardiac damage.
Conclusion: Our findings suggested that NAC may be a potential radioprotector which is capable of preventing cardiac damage.
PMID: 31990607 [PubMed - as supplied by publisher] (Source: International Journal of Radiation Biology)
Source: International Journal of Radiation Biology - January 29, 2020 Category: Radiology Tags: Int J Radiat Biol Source Type: research
p38 MAP-kinase inhibitor protects against platelet-activating factor-induced death in mice.
Abstract
Platelet-activating factor (PAF) is a potent inflammatory agonist. In Swiss albino mice, intraperitoneal injection of PAF causes sudden death with oxidative stress and disseminated intravascular coagulation (DIC), characterized by prolonged prothrombin time, thrombocytopenia, reduced fibrinogen content, and increased levels of fibrinogen degradation products. However, the underlying mechanism (s) is unknown. The PAF-R antagonist WEB-2086 protected mice against PAF-induced death by reducing DIC and oxidative stress. Accordingly, general antioxidants such as ascorbic acid, α-tocopherol, gallic acid...
Source: Free Radical Biology and Medicine - August 19, 2019 Category: Biology Authors: Abhilasha KV, Sumanth MS, Chaithra VH, Jacob SP, Thyagarajan A, Sahu RP, Rajaiah R, Prabhu KS, Kemparaju K, Travers JB, Chen CH, Marathe GK Tags: Free Radic Biol Med Source Type: research
Tolerizing CTL by Sustained Hepatic PD-L1 Expression Provides a New Therapy Approach in Mouse Sepsis
Conclusion: These results suggest that, contrary to common assumption, maintaining PD-L1 expression on hepatocytes improves liver damage and survival of mice during sepsis. We conclude that administering recombinant PD-L1 or inhibiting NOX2 activity might offer a new therapeutic option in sepsis. (Source: Theranostics)
Source: Theranostics - June 13, 2019 Category: Molecular Biology Authors: Andreas von Knethen, Anne Sch ä fer, Laura Kuchler, Tilo Knape, Urs Christen, Edith Hintermann, Beate Fi ß lthaler, Katrin Schr ö der, Ralf P. Brandes, Berit Genz, Kerstin Abshagen, Brigitte M. P ü tzer, Lisa K. Sha, Andreas Weigert, Shahzad N. Syed, Tags: Research Paper Source Type: research
1H NMR profiling of the 6-OHDA parkinsonian rat brain reveals metabolic alterations and signs of recovery after N-acetylcysteine treatment
Publication date: Available online 12 June 2019Source: Molecular and Cellular NeuroscienceAuthor(s): Ana Virel, Ilona Dudka, Rutger Laterveer, Sara af BjerkénAbstractParkinson's disease is the second most common neurodegenerative disease caused by degeneration of dopamine neurons in the substantia nigra. The origin and causes of dopamine neurodegeneration in Parkinson's disease are not well understood but oxidative stress may play an important role in its onset. Much effort has been dedicated to find biomarkers indicative of oxidative stress and neurodegenerative processes in parkinsonian brains. By using 1H NMR (nuclear ...
Source: Molecular and Cellular Neuroscience - June 12, 2019 Category: Neuroscience Source Type: research
Acetylcysteine-Decorated Prussian Blue Nanoparticles for Strong Photothermal Sterilization and Focal Infection Treatment
Publication date: Available online 7 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Suya Cai, Jun Qian, Sen Yang, Liangju Kuang, Daoben HuaAbstractThe major challenge in bacterial infection in clinical settings is the development of antimicrobial materials in the treatment of drug-resistant bacteria. Herein, we report a new strategy for efficient near-infrared radiation (NIR) photothermal sterilization and focal infection treatment by acetylcysteine-modified Prussian blue nanoparticles (AC-PB). Specifically, AC-PB is fabricated as a multifunctional therapeutic agent via a co-precipitation approach, where ...
Source: Colloids and Surfaces B: Biointerfaces - May 17, 2019 Category: Biochemistry Source Type: research
Is Aberrant Reno-Renal Reflex Control of Blood Pressure a Contributor to Chronic Intermittent Hypoxia-Induced Hypertension?
This study demonstrated unaltered vascular conductance in response to lumbar sympathetic stimulation in CIH-exposed rats. Aortic compliance was increased and estimated blood volume was unchanged in CIH-exposed rats. Increased blood pressure was related to an increase in cardiac output, which was confirmed by echocardiography (Lucking et al., 2014). It is suggested therefore that hypertension in the CIH model can be evoked by over-excitation of the cardiac arm of sympathetic nervous system (SNS), even before mechanisms of enhanced peripheral vasoconstriction and endothelial dysfunction are initiated (Naghshin et al., 2009)....
Source: Frontiers in Physiology - April 23, 2019 Category: Physiology Source Type: research
Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer ’s Disease Through Activation of BDNF-TrkB Signaling Pathway
Conclusion
The data in this manuscript demonstrates that apelin-13 upregulates BDNF against STZ-induced congnitive impairment by suppressing glial cell activity and inflammatory factors release. This suggests apelin signaling may be a new target in the treatment of AD.
Ethics Statement
All experimental protocols were carried out according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals approved by the Central South University at XiangYa Animal Care and Use Committee.
Author Contributions
XqQ and LH conceived the study and contributed to its experimental design. HqL carried out the...
Source: Frontiers in Pharmacology - April 15, 2019 Category: Drugs & Pharmacology Source Type: research
Oral Cannabidiol Prevents Allodynia and Neurological Dysfunctions in a Mouse Model of Mild Traumatic Brain Injury
Conclusion
In conclusion, our data demonstrate that mTBI causes late sensorial affective/cognitive deficiencies linked to altered neurotransmitter release at cortical level. Moreover, we showed that chronic CBD treatment reduces behavioral dysfunctions by restoring at least in part cortical biochemical processes. Taken together, our results suggest that CBD could represent a novel approach for the management of neuropsychiatric disorders associated with TBI.
Author Contributions
CB, LL, and RR conceived and designed the experiments. CB, MI, SB, FR, RI, and RM performed the experiments. GP, LS, SP, RR, LL, and IM analyze...
Source: Frontiers in Pharmacology - April 15, 2019 Category: Drugs & Pharmacology Source Type: research
Thymoquinone Attenuates Acetaminophen Overdose-Induced Acute Liver Injury and Inflammation Via Regulation of JNK and AMPK Signaling Pathway.
Abstract
Thymoquinone (TQ) is a main aromatic component of Nigella sativa L. seeds or Agastache rugosa (Fisch. & C.A.Mey.) Kuntze. The protective mechanism of TQ against acute liver injury induced by acetaminophen (APAP), however, remains unclear. We aimed to investigated the hepato-protective mechanism of TQ on the development of APAP-induced acute liver injury. Male kunming mice were pretreated with TQ or N-acetylcysteine (NAC) before a single APAP injection. Human Chang liver cells were incubated with TQ, SP600125 or AICAR in presence of APAP for 24 h. TQ pretreatment reduced levels of serum aminot...
Source: The American Journal of Chinese Medicine - April 10, 2019 Category: Complementary Medicine Authors: Cui BW, Bai T, Yang Y, Zhang Y, Jiang M, Yang HX, Wu M, Liu J, Qiao CY, Zhan ZY, Wu YL, Kang DZ, Lian LH, Nan JX Tags: Am J Chin Med Source Type: research
