Effect of N-acetylcysteine on hair follicle changes in mouse model of cyclophosphamide-induced alopecia: histological and biochemical study
AbstractChemotherapy-induced alopecia (CIA) represents one of the most severe side effects of chemotherapy, which forces some patients to reject cancer treatment. The exact pathophysiological mechanisms of CIA are not clearly understood, which makes it difficult to discover efficient preventive or therapeutic procedures for this adverse effect. N-acetylcysteine (NAC) has a strong antioxidant activity as it stimulates glutathione synthesis and acts as an oxygen radical scavenger. The current study tried to investigate the efficacy of NAC in preserving biochemical parameters and hair follicle structure against cyclophosphami...
Source: Histochemistry and Cell Biology - April 20, 2024 Category: Biomedical Science Source Type: research
Analysis of Gizzerosine in Foodstuffs by HPLC Involving Pre-column Derivatization with o-Phthaldialdehyde
Adv Exp Med Biol. 2024;1446:237-250. doi: 10.1007/978-3-031-54192-6_11.ABSTRACTGizzerosine [2-amino-9-(4-imidazolyl)-7-azanonanoic acid] is a toxic amino acid formed from histamine and lysine at high temperatures, and may be present in foodstuffs (e.g., fishmeal and meat-bone meal) for animals including cats and dogs. Here we developed a simple, rapid, sensitive, specific, and automated method for the analysis of gizzerosine in foodstuffs by high-performance liquid chromatography (HPLC) involving pre-column derivatization with o-phthaldialdehyde (OPA) in the presence of N-acetylcysteine (instead of the usual 2-mercaptoetha...
Source: Advances in Experimental Medicine and Biology - April 16, 2024 Category: Research Authors: Peng Li Guoyao Wu Source Type: research
In Vivo Intracerebral Administration of α-Ketoisocaproic Acid to Neonate Rats Disrupts Brain Redox Homeostasis and Promotes Neuronal Death, Glial Reactivity, and Myelination Injury
AbstractMaple syrup urine disease (MSUD) is caused by severe deficiency of branched-chain α-keto acid dehydrogenase complex activity, resulting in tissue accumulation of branched-chain α-keto acids and amino acids, particularly α-ketoisocaproic acid (KIC) and leucine. Affected patients regularly manifest with acute episodes of encephalopathy including seizures, coma, and potentially f atal brain edema during the newborn period. The present work investigated the ex vivo effects of a single intracerebroventricular injection of KIC to neonate rats on redox homeostasis and neurochemical markers of neuronal viability (neuron...
Source: Molecular Neurobiology - November 1, 2023 Category: Neurology Source Type: research
Molecules, Vol. 28, Pages 6762: N-Acetylcysteine Displaces Glutathionyl-Moieties from Hg2+ and MeHg+ to Form More Hydrophobic Complexes at Near-Physiological Conditions
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The anthropogenic release of Hg is associated with an increased human exposure risk. Since Hg2+ and MeHg+ have a high affinity for thiols, their interaction with L-glutathione (GSH) within mammalian cells is fundamentally involved in their toxicological chemistry and excretion. To gain insight into the interaction of these mercurials with multiple small molecular weight thiols, we have investigated their competitive interactions with GSH and N-acetylcysteine (NAC) at near-physiological conditions, using a liquid chromatographic approach. This approach involved the injection of each mercurial onto a reversed-phase ...
Source: Molecules - September 22, 2023 Category: Chemistry Authors: Maryam Doroudian Michelle E. Thibault J ürgen Gailer Tags: Article Source Type: research
Determination of o-phthalaldehyde for dose verification of the clinical disinfectant by fluorescent sequential injection analysis
Anal Sci. 2023 Aug 26. doi: 10.1007/s44211-023-00415-4. Online ahead of print.ABSTRACTA new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075-0.02%). As a result, excellent linearity (R2 > 0.99) and pre...
Source: Anal Sci - August 26, 2023 Category: Chemistry Authors: Hung-Yu Chen Richie L C Chen Bo-Chuan Hsieh Tzong-Jih Cheng Source Type: research
Determination of o-phthalaldehyde for dose verification of the clinical disinfectant by fluorescent sequential injection analysis
Anal Sci. 2023 Aug 26. doi: 10.1007/s44211-023-00415-4. Online ahead of print.ABSTRACTA new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075-0.02%). As a result, excellent linearity (R2 > 0.99) and pre...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - August 26, 2023 Category: Chemistry Authors: Hung-Yu Chen Richie L C Chen Bo-Chuan Hsieh Tzong-Jih Cheng Source Type: research
Determination of o-phthalaldehyde for dose verification of the clinical disinfectant by fluorescent sequential injection analysis
Anal Sci. 2023 Aug 26. doi: 10.1007/s44211-023-00415-4. Online ahead of print.ABSTRACTA new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075-0.02%). As a result, excellent linearity (R2 > 0.99) and pre...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - August 26, 2023 Category: Chemistry Authors: Hung-Yu Chen Richie L C Chen Bo-Chuan Hsieh Tzong-Jih Cheng Source Type: research
Determination of o-phthalaldehyde for dose verification of the clinical disinfectant by fluorescent sequential injection analysis
Anal Sci. 2023 Aug 26. doi: 10.1007/s44211-023-00415-4. Online ahead of print.ABSTRACTA new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075-0.02%). As a result, excellent linearity (R2 > 0.99) and pre...
Source: Anal Sci - August 26, 2023 Category: Chemistry Authors: Hung-Yu Chen Richie L C Chen Bo-Chuan Hsieh Tzong-Jih Cheng Source Type: research
An integrated analytical strategy to decipher the metabolic profile of alkaloids in Compound Kushen injection based on UHPLC-ESI-QTOF/MS < sup > E < /sup >
Xenobiotica. 2023 Jun 19:1-29. doi: 10.1080/00498254.2023.2227976. Online ahead of print.ABSTRACT1. Compound Kushen injection (CKI) is a kind of sterilized water-soluble traditional Chinese medicine preparation that has been used for the clinical treatment of a variety of cancers (hepatocellular carcinoma, lung cancer, etc.) for nineteen years. However, to date, the metabolism-related study on CKI in vivo has not been conducted.2. An integrated analytical strategy was established to investigate the metabolic profile of alkaloids of CKI in rat plasma, urine and feces based on ultra-high performance liquid chromatography-ele...
Source: Xenobiotica - June 19, 2023 Category: Research Authors: Li Zhang Ruijuan Li Ting Zheng Huan Wu Yanyan Yin Source Type: research
An integrated analytical strategy to decipher the metabolic profile of alkaloids in Compound Kushen injection based on UHPLC-ESI-QTOF/MS < sup > E < /sup >
Xenobiotica. 2023 Jun 19:1-29. doi: 10.1080/00498254.2023.2227976. Online ahead of print.ABSTRACT1. Compound Kushen injection (CKI) is a kind of sterilized water-soluble traditional Chinese medicine preparation that has been used for the clinical treatment of a variety of cancers (hepatocellular carcinoma, lung cancer, etc.) for nineteen years. However, to date, the metabolism-related study on CKI in vivo has not been conducted.2. An integrated analytical strategy was established to investigate the metabolic profile of alkaloids of CKI in rat plasma, urine and feces based on ultra-high performance liquid chromatography-ele...
Source: Xenobiotica - June 19, 2023 Category: Research Authors: Li Zhang Ruijuan Li Ting Zheng Huan Wu Yanyan Yin Source Type: research
An integrated analytical strategy to decipher the metabolic profile of alkaloids in Compound Kushen injection based on UHPLC-ESI-QTOF/MS < sup > E < /sup >
Xenobiotica. 2023 Jun 19:1-29. doi: 10.1080/00498254.2023.2227976. Online ahead of print.ABSTRACT1. Compound Kushen injection (CKI) is a kind of sterilized water-soluble traditional Chinese medicine preparation that has been used for the clinical treatment of a variety of cancers (hepatocellular carcinoma, lung cancer, etc.) for nineteen years. However, to date, the metabolism-related study on CKI in vivo has not been conducted.2. An integrated analytical strategy was established to investigate the metabolic profile of alkaloids of CKI in rat plasma, urine and feces based on ultra-high performance liquid chromatography-ele...
Source: Xenobiotica - June 19, 2023 Category: Research Authors: Li Zhang Ruijuan Li Ting Zheng Huan Wu Yanyan Yin Source Type: research
Activation of neuronal NADPH oxidase NOX2 promotes inflammatory neurodegeneration
This study aimed to investigate expression patterns, regulatory mechanisms and pathological roles of neuronal NADPH oxidase in inflammation-associated neurodegeneration. The results showed persistent upregulation of NOX2 (gp91phox; the catalytic subunit of NADPH oxidase) in both microglia and neurons in a chronic mouse model of Parkinson's disease (PD) with intraperitoneal LPS injection and LPS-treated midbrain neuron-glia cultures (a cellular model of PD). Notably, NOX2 was found for the first time to exhibit a progressive and persistent upregulation in neurons during chronic neuroinflammation. While primary neurons and N...
Source: Free Radical Biology and Medicine - March 4, 2023 Category: Biology Authors: Dezhen Tu Ravikanth Velagapudi Yun Gao Jau-Shyong Hong Hui Zhou Hui-Ming Gao Source Type: research
Activation of neuronal NADPH oxidase NOX2 promotes inflammatory neurodegeneration
This study aimed to investigate expression patterns, regulatory mechanisms and pathological roles of neuronal NADPH oxidase in inflammation-associated neurodegeneration. The results showed persistent upregulation of NOX2 (gp91phox; the catalytic subunit of NADPH oxidase) in both microglia and neurons in a chronic mouse model of Parkinson's disease (PD) with intraperitoneal LPS injection and LPS-treated midbrain neuron-glia cultures (a cellular model of PD). Notably, NOX2 was found for the first time to exhibit a progressive and persistent upregulation in neurons during chronic neuroinflammation. While primary neurons and N...
Source: Free Radical Biology and Medicine - March 4, 2023 Category: Biology Authors: Dezhen Tu Ravikanth Velagapudi Yun Gao Jau-Shyong Hong Hui Zhou Hui-Ming Gao Source Type: research
Yinhuang oral liquid protects acetaminophen-induced acute liver injury by regulating the activation of autophagy and Nrf2 signaling
In conclusion, YOL had a protective and therapeutic role in APAP-induced liver injury in mice by activating Nrf2 signaling pathway and autophagy.PMID:36115150 | DOI:10.1016/j.ecoenv.2022.114073 (Source: Ecotoxicology and Environmental Safety)
Source: Ecotoxicology and Environmental Safety - September 17, 2022 Category: Environmental Health Authors: Yong-Ming He Xing-Ling Shen Yan-Na Guo Shao-Shan Liang Kang-Ning Ding Meng-Han Lu Lu-Ping Tang Source Type: research
