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Cancer: Colon Cancer

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Total 665 results found since Jan 2013.

Effect of combination therapy of siRNA targeting growth hormone receptor and 5-fluorouracil in hepatic metastasis of colon cancer.
Authors: Zhou D, Zhang YI, Liang D, Yuan Y, Zeng D, Chen J, Yang J Abstract The aim of this study was to investigate the effects of small interfering RNA (siRNA) targeting human growth hormone receptor (hGHR) combined with 5-fluorouracil (5-FU) on the hepatic metastasis of colon cancer. The animal model of liver metastases using human SW480 colon cancer cells was established on BALB/c mice and the siRNA interfering plasmid targeting hGHR gene was constructed. The tumor-bearing mice were randomly divided into the saline control, plasmid, growth hormone (GH), 5-FU, 5-FU+plasmid and 5-FU+plasmid+GH groups. The liver m...
Source: Oncology Letters - January 21, 2016 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Specific Delivery of Delta-5-Desaturase siRNA via RNA Nanoparticles Supplemented with Dihomo-γ-Linolenic Acid for Colon Cancer Suppression
In this study, we employed RNA nanotechnology for specific delivery of D5D-siRNA to xenograft colon tumors using 3WJ RNA nanoparticles. When a targeting module, i.e., the EpCAM aptamer, was incorporated, the 3WJ pRNA nanoparticles were able specifically deliver D5D siRNA to human colon cancer HCA-7 cells both in vitro and in vivo, resulting in significant downregulation of D5D expression. Co-treatment with DGLA in combination with 3WJ-EpCAM-siRNA induced a higher DGLA/AA ratio and enhanced formation of 8-HOA at a threshold level, and in HCA-7 tumor-bearing mice, induced significant tumor suppression. We further confirmed t...
Source: Redox Biology - December 19, 2018 Category: Biology Source Type: research

Peptide generated anisotropic gold nanoparticles as efficient siRNA vectors
In conclusion, our results demonstrate that peptide -GNPs based delivery system for siRNA emerges to be effective to deliver RNAi therapeutics, uncovering new avenue in oncotherapy.Graphical abstractPeptide generated anisotropic gold nanoparticles are demonstrated as efficient siRNA carriers. Delivered GAPDH siRNA exhibits significant GAPDH gene suppression in colon cancer cells.
Source: International Journal of Pharmaceutics - April 4, 2019 Category: Drugs & Pharmacology Source Type: research

Effect of siRNA-mediated knockdown of eIF3c gene on survival of colon cancer cells.
This study was focused on investigating the effect of small interfering RNA (siRNA)-mediated eIF3c gene knockdown on colon cancer cell survival. The eIF3c gene was observed to be highly expressed in colon cancer cell models. The expression levels of the gene in eIF3c siRNA infected and control siRNA infected cells were compared via real-time polymerase chain reaction (PCR) and western blotting analysis. Cell proliferation levels were analyzed employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays. Furthermore, the effects of eIF3c gene knockdown on the cell cycle and apopto...
Source: J Zhejiang Univ Sci ... - June 1, 2013 Category: Science Authors: Song N, Wang Y, Gu XD, Chen ZY, Shi LB Tags: J Zhejiang Univ Sci B Source Type: research

BACH1 silencing by siRNA inhibits migration of HT-29 colon cancer cells through reduction of metastasis-related genes.
CONCLUSION: Our results indicated that BACH1 down-regulation in HT29 CRC cells had no effect on cell growth but did inhibit cell migration by decreasing metastasis-related genes expression. Collectively, these results suggest that BACH1 may function as an oncogenic driver in colon cancer and may represent as a potential target of gene therapy for CRC treatment. PMID: 27657827 [PubMed - as supplied by publisher]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - September 18, 2016 Category: Drugs & Pharmacology Authors: Davudian S, Shajari N, Kazemi T, Mansoori B, Salehi S, Mohammadi A, Shanehbandi D, Shahgoli VK, Asadi M, Baradaran B Tags: Biomed Pharmacother Source Type: research

Extracellular vesicles from human umbilical cord mesenchymal stem cells treated with siRNA against ELFN1-AS1 suppress colon adenocarcinoma proliferation and migration.
This study aimed to explore the regulatory role of ELFN1-AS1 in COAD and construct a gene delivery system based on extracellular vesicles (EVs). We found that ELFN1-AS1 levels were obviously increased in COAD patients and COAD tumor cells. Knockdown of ELFN1-AS1 expression by siRNA inhibited COAD cell proliferation and migration. Moreover, silencing ELFN1-AS1 significantly reduced the activation of extracellular signal-regulated protein kinase (Erk), up-regulated the protein expression of E-cadherin and down-regulated vimentin. In addition, we treated human umbilical cord mesenchymal stem cells (hUCMSCs) with siRNA-ELFN1-A...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

NEAT1 siRNA Packed with Chitosan Nanoparticles Regulates the Development of Colon Cancer Cells via lncRNA NEAT1/miR-377-3p Axis
This study was for verifying that transfecting colon cancer cells (CCCs) with lncRNA NEAT1 packed with siRNA chitosan nanoparticles (CNPs) can suppress lncRNA NEAT1 and biological behaviors of the cells. siRNA targeting lncRNA NEAT1 expression vector was constructed and then transfected into CCCs after being packed with CNPs. Subsequently, the impact of the transfection on biological behaviors of the cells was evaluated. As a result, with high expression in CCCs, NEAT1 was negatively bound up with miR-377-3p in cases with colon cancer (CC), and dual luciferase reporter assay confirmed the potential binding region. Addition...
Source: Biomed Res - May 31, 2021 Category: Research Authors: Tianyu Li Nenghui Deng Ruimei Xu Zhihao Fan Junli He Zirun Zheng Hailian Deng Riyu Liao Xinqi Lv Cailian Pang Source Type: research

Demonstration of dose dependent cytotoxic activity in SW480 colon cancer cells by 177Lu-labeled siRNA targeting IGF-1R
In this study, radioconjugate of IGF-1R siRNA with lutetium-177 (177Lu) was produced, and the anti-proliferation and apoptosis effects elicited by this 177Lu-siRNA complex in the SW480 colon cancer cells were evaluated.Methods: IGF-1R and Luciferase siRNAs were conjugated with p-SCN-Bn-DTPA, and then radiolabeled with 177Lu. The effects of labeled and non-labeled IGF-1R siRNAs on IGF-1R expression were assessed with RT-PCR analysis and ELISA assay. IGF-1R siRNAs induced cell death and apoptosis were investigated using MTT assay and Annexin-V/propidium iodide (PI) double staining, respectively.Results: Combined purification...
Source: Nuclear Medicine and Biology - April 27, 2013 Category: Radiology Authors: Mojtaba Fathi, Mohammad Taghikhani, Mohammad Ghannadi-Maragheh, Kamal Yavari Source Type: research

A novel therapeutic drug for colon cancer: EpCAM scFv-truncated protamine (tp)-siRNA.
Abstract Colon cancer is a type of malignant tumor that causes considerable mortality worldwide. Epithelial cellular adhesion molecule (EpCAM), a tumor-associated antigen of colon tumors, is a target for colon cancer therapy. EpCAM-specific monoclonal antibodies (mAbs) have been applied in human colon cancer since the 1990s; however, the therapeutic effects are limited. EpCAM activates nuclear signaling pathways by releasing its intracellular domain (EpICD). The released EpICD stimulates the Wnt/β-catenin signaling pathway, which is also strongly associated with tumorigenesis. EpCAM is also a target gene of the W...
Source: Cell Biology International - April 10, 2013 Category: Cytology Authors: Hao H, Zhen Y, Wang Z, Chen F, Xie X Tags: Cell Biol Int Source Type: research

Abstract 3373: Identification of inflammation-related genes that regulate tumor-associated stemness using high-throughput siRNA screening
Cancer associated stem cells (CSCs) are considered to be responsible for tumor recurrence, metastasis as well as chemoresistance. These properties of tumor associated stemness are maintained by specialized microenvironment, including inflammation which is a key component of the tumor microenvironment. To study the relationship between inflammation and stemness of CSCs, here, we established a robust high-throughput RNAi screen platform for a global survey of inflammation-related genes affecting CSCs identity via alteration of Oct4 expression. Consequently, we found 72 novel genes from 1027 inflammation-related genes which c...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Xie, J., He, H., Chen, C., Bai, L., Wang, W., Liu, Y., Guo, J., Wu, P., Xiang, R., Luo, Y. Tags: Tumor Biology Source Type: research

BACH1 silencing by siRNA inhibits migration of HT-29 colon cancer cells through reduction of metastasis-related genes
Conclusion Our results indicated that BACH1 down-regulation in HT29 CRC cells had no effect on cell growth but did inhibit cell migration by decreasing metastasis-related genes expression. Collectively, these results suggest that BACH1 may function as an oncogenic driver in colon cancer and may represent as a potential target of gene therapy for CRC treatment.
Source: Biomedicine and Pharmacotherapy - September 19, 2016 Category: Drugs & Pharmacology Source Type: research

Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
Background: Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC. Methods: The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell prolif...
Source: Cancer Cell International - February 5, 2013 Category: Cancer & Oncology Authors: Bo HuangHongli ZhouXiaodong WangZhiliang Liu Source Type: research

PD-1-siRNA Delivered by Attenuated Salmonella Enhances the Antitumor Effect of Chloroquine in Colon Cancer
This study suggests that the combined application of PD-1-based immunotherapy and anti-cancer drugs has become a new expectation for clinical treatment of colorectal cancer.
Source: Frontiers in Immunology - July 6, 2021 Category: Allergy & Immunology Source Type: research

siRNA-targeted inhibition of growth hormone receptor in human colon cancer SW480 cells.
CONCLUSION: Inhibition of GHR in human colon cancer SW480 cells resulted in anti-tumor effects in nude mice. PMID: 24307807 [PubMed - in process]
Source: World Journal of Gastroenterology : WJG - November 28, 2013 Category: Gastroenterology Authors: Zhou D, Yang J, Huang WD, Wang J, Zhang Q Tags: World J Gastroenterol Source Type: research