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Cancer: Colon Cancer

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Total 665 results found since Jan 2013.

Nucleophosmin 1, upregulated in adenomas and cancers of the colon, inhibits p53‐mediated cellular senescence
Abstract Dysregulation of nucleophosmin 1 (NPM1) has been found in numerous solid and hematological malignancies. Our previous meta‐analysis of colorectal cancer (CRC) high throughput gene expression profiling studies identified it as a consistently reported up‐regulated gene in the malignant state. Our aims were to compare NPM1 expression in normal colon, adenoma and CRC, to correlate their expressions with clinico‐pathological parameters, and to assess the biological role of aberrant NPM1 expression in CRC cells. NPM1 transcript levels were studied in human CRC cell lines, whereas a tissue microarray of 57 normal h...
Source: International Journal of Cancer - March 28, 2013 Category: Cancer & Oncology Authors: John CT Wong, Mohammad R Hasan, Mahbuba Rahman, Angel C Yu, Simon K Chan, David F Schaeffer, Hagen F Kennecke, Howard J Lim, David Owen, Isabella T Tai Tags: Cancer Cell Biology Source Type: research

Prospective of colon cancer treatments and scope for combinatorial approach to enhanced cancer cell apoptosis
Abstract: Colorectal cancer is the leading cause of cancer-related mortality in the western world. It is also the third most common cancer diagnosed in both men and women in the United States with a recent estimate for new cases of colorectal cancer in the year 2012 being around 103,170. Various risk factors for colorectal cancer include life-style, diet, age, personal and family history, and racial and ethnic background. While a few cancers are certainly preventable but this does not hold true for colon cancer as it is often detected in its advanced stage and generally not diagnosed until symptoms become apparent. Despite...
Source: Critical Reviews in Oncology Hematology - October 24, 2012 Category: Cancer & Oncology Authors: Jayshree Mishra, Joseph Drummond, Sohel H. Quazi, Satya Sridhar Karanki, J.J. Shaw, Ben Chen, Narendra Kumar Source Type: research

In-depth characterization of the secretome of colorectal cancer metastatic cells identifies key proteins in cell adhesion, migration and invasion.
Abstract Liver metastasis in colorectal cancer is the major cause of cancer related death. To identify and characterize proteins associated with colon cancer metastasis, we have compared the conditioned serum-free medium of highly metastatic KM12SM colorectal cancer cells with the parental, poorly metastatic, KM12C cells using quantitative SILAC analyses on a linear ion trap-Orbitrap Velos mass spectrometer. In total, 1337 proteins were simultaneously identified in SILAC forward and reverse experiments. For quantification, 1098 proteins were selected in both experiments, with 155 proteins showing >1.5 fold-chan...
Source: Molecular and Cellular Proteomics : MCP - February 26, 2013 Category: Molecular Biology Authors: Barderas R, Mendes M, Torres S, Bartolome RA, Lopez-Lucendo M, Villar-Vazquez R, Pelaez A, Fuente E, Bonilla F, Casal JI Tags: Mol Cell Proteomics Source Type: research

Hydroxycamptothecin induces apoptosis and inhibits tumor growth in colon cancer by the downregulation of survivin and XIAP expression
Conclusions: Our results elucidate new mechanisms of HCPT antitumor by the downregulation of survivin and XIAP expression. The combination of HCPT with 5-FU or IAP inhibitors may be a potential strategy for colon cancer treatment.
Source: World Journal of Surgical Oncology - May 30, 2013 Category: Cancer & Oncology Authors: Bojian FeiAlfred ChiYuan Weng Source Type: research

MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells.
Abstract Histone deacetylase (HDAC) inhibitors have recently emerged as a new class of anti-cancer agents. Trichostatin A (TSA), a classical HDAC inhibitor, has been demonstrated to induce cell cycle arrest, promote cell apoptosis, and inhibit metastasis. However, the molecular mechanism underlying TSA function has not been fully elucidated. In the current study, we found that TSA treatment induced altered expression of cell cycle-associated genes in HCT116 cells by RT-PCR array. Among the 84 genes related to cell cycle control, 34 genes were significantly altered by TSA treatment, with 7 genes upregulated and 27 ...
Source: Toxicology Letters - June 13, 2013 Category: Toxicology Authors: Liu Y, He G, Wang Y, Guan X, Pang X, Zhang B Tags: Toxicol Lett Source Type: research

Activation of p53 pathway by Nutlin-3a inhibits the expression of the therapeutic target α5 integrin in colon cancer cells
Highlights: Abstract: Integrins emerge nowadays as crucial actors of tumor aggressiveness and resistance to therapies. Integrin α5β1, the fibronectin receptor, determines malignant properties of colon carcinoma which is one of the most important causes of cancer-related deaths in the world. Here we show that inhibition of α5 integrin subunit expression by siRNA or α5β1 integrin function by specific antagonist affects the survival of HCT116 colon cancer cells. We also evidence that pharmacological reactivation of the tumor suppressor p53 by Nutlin-3a inhibits specifically the expression of the α5 integrin subunit both...
Source: Cancer Letters - April 11, 2013 Category: Cancer & Oncology Authors: Hana Janouskova, Anne-Marie Ray, Fanny Noulet, Isabelle Lelong-Rebel, Laurence Choulier, Florence Schaffner, Maxime Lehmann, Sophie Martin, Jan Teisinger, Monique Dontenwill Tags: Research Articles Source Type: research

Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells.
Abstract Major vault protein (MVP) is identical to lung resistance-related protein (LRP), which is the major component of vaults. Vaults are considered to play a protective role against xenobiotics and other types of stress. In a previous study, we reported that the expression levels of MVP in SW620 human colon cancer cells were increased in hypertonic culture medium with sucrose. However, the molecular mechanism behind the induction of MVP expression by osmotic stress has not yet been elucidated. Therefore, in the present study, we investigated the mechanism behind the induction of MVP expression by osmotic stres...
Source: International Journal of Molecular Medicine - July 2, 2013 Category: Molecular Biology Authors: Tajitsu Y, Ikeda R, Nishizawa Y, Mataki H, Che XF, Sumizawa T, Nitta M, Yamaguchi T, Yamamoto M, Tabata S, Akiyama SI, Yamada K, Furukawa T, Takeda Y Tags: Int J Mol Med Source Type: research

The cysteinyl leukotriene 2 receptor contributes to all-trans retinoic acid-induced differentiation of colon cancer cells
Conclusions: This study identifies a novel mechanism of action for ATRA in colorectal cancer cell differentiation and demonstrates that retinoids can have anti-tumorigenic effects through their action on the cysteinyl leukotriene pathway.
Source: BMC Cancer - July 8, 2013 Category: Cancer & Oncology Authors: Astrid BengtssonGunilla JönssonCecilia MagnussonTavga SalimCecilia AxelssonAnita Sjölander Source Type: research

Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells.
CONCLUSION: Genes of the proteins that interacted with 14-3-3σ were successfully screened from a HeLa cDNA library. KCMF1 and 14-3-3σ protein may affect the proliferation and colony formation of human colon cancer stem cells. PMID: 23840115 [PubMed - in process]
Source: World Journal of Gastroenterology : WJG - June 28, 2013 Category: Gastroenterology Authors: Zou J, Mi L, Yu XF, Dong J Tags: World J Gastroenterol Source Type: research

Adaptive Response Diminishes Therapeutic Effectiveness
Adaptive responses can be induced in cells by very low doses of ionizing radiation resulting in an enhanced resistance to much larger exposures. The inhibitor of apoptosis protein, survivin, has been implicated in many adaptive responses to cellular stress. Computerized axial tomography used in image-guided radiotherapy to position and monitor tumor response uses very low radiation doses ranging from 0.5 to 100 mGy. We investigated the ability of these very low radiation doses administered along with two 2 Gy doses separated by 24 hours, a standard conventional radiotherapy dosing schedule, to initiate adaptive responses r...
Source: Cancer Research - July 15, 2013 Category: Cancer & Oncology Authors: Grdina, D. J., Murley, J. S., Miller, R. C., Mauceri, H. J., Sutton, H. G., Li, J. J., Woloschak, G. E., Weichselbaum, R. R. Tags: Therapeutics, Targets, and Chemical Biology Source Type: research

A Kinome-Wide siRNA Screen Identifies Multiple Roles for Protein Kinases in Hypoxic Stress Adaptation, Including Roles for IRAK4 and GAK in Protection against Apoptosis in VHL-/- Renal Carcinoma Cells, Despite Activation of the NF-{kappa}B Pathway
Hypoxia induces changes to cancer cells that make them more resistant to treatment. We have looked at signaling pathways that facilitate these changes by screening the human kinome for effects on hypoxic responses in SW480 colon cancer cells. Hits identified in the screen were examined for effects on multiple molecular responses to hypoxia, including the endoplasmic reticulum stress and DNA damage responses in colon, melanoma, and renal cancer lines. To validate the hits from the small interfering RNA studies, we developed cell lines expressing stable short hairpin RNAs (shRNAs) in the A498 renal carcinoma cell line. Sever...
Source: Journal of Biomolecular Screening - July 23, 2013 Category: Molecular Biology Authors: Pan, J., Zhang, J., Hill, A., Lapan, P., Berasi, S., Bates, B., Miller, C., Haney, S. Tags: Original Research Source Type: research

Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway
This study aimed to demonstrate the effect of brucine on tumour angiogenesis and its mechanism of action. The anti‐angiogenic effect was evaluated on the chicken chorioallantoic membrane (CAM) model and tube formation. The mechanism was demonstrated through detecting mRNA and protein expressions of VEGFR2 (KDR), PKCα, PLCγ and Raf1 by reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blot (WB), as well as expressions of VEGF and PKCβ and mTOR by ELISA and WB. The results showed that brucine significantly reduced angiogenesis of CAM and tube formation, inhibited the VEGF secretion and mTOR expres...
Source: Journal of Cellular and Molecular Medicine - August 2, 2013 Category: Molecular Biology Authors: Wenjuan Luo, Xiaoli Wang, Lei Zheng, Yingzhuan Zhan, Dongdong Zhang, Jie Zhang, Yanmin Zhang Tags: Original Article Source Type: research

Role of Hypoxia Inducible Factor-1α in the Regulation of the Cancer-Specific Variant of Organic Anion Transporting Polypeptide 1B3 (OATP1B3), in Colon and Pancreatic Cancer.
Abstract Organic anion transporting polypeptide 1B3 (OATP1B3) was initially considered to be a liver-specific transporter, mediating the uptake of a variety of endogenous and xenobiotic substances. Over the past decade, several investigations reported that OATP1B3 is also expressed across multiple types of cancers. Only recently, our laboratory and others demonstrated the identity of cancer-specific OATP1B3 variants (csOATP1B3) arising from the use of an alternative transcription initiation site, different from the wildtype (WT) OATP1B3 expressed in the normal liver. However, the mechanisms regulating the expressi...
Source: Biochemical Pharmacology - August 4, 2013 Category: Drugs & Pharmacology Authors: Han S, Kim K, Thakkar N, Kim D, Lee W Tags: Biochem Pharmacol Source Type: research

HnRNPK Regulates 3' End RNA Processing at EGR1 DNA and Chromosomes
The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a nucleic acid-binding protein that acts as a docking platform integrating signal transduction pathways to nucleic acid-related processes. Given that hnRNPK could be involved in other steps that compose gene expression the definition of its genome-wide occupancy is important to better understand its role in transcription and co-transcriptional processes. Here, we used chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) to analyze the genome-wide hnRNPK-DNA interaction in colon cancer cell line HCT116. 9.1/3.6 and 7.0/3.4 million tags were sequenced/...
Source: Journal of Biological Chemistry - August 23, 2013 Category: Chemistry Authors: Mikula, M., Bomsztyk, K., Goryca, K., Chojnowski, K., Ostrowski, J. Tags: Gene Regulation Source Type: research

Crosstalk between colon cancer cells and macrophages via inflammatory mediators and CD47 promotes tumour cell migration
Abstract: Tumour-associated macrophages (TAMs) of the M2 phenotype are present in the stroma of many tumours and are frequently associated with the progression of several types of cancer. We investigated the role of M2 macrophages in colon cancer progression and found that human colon cancer tissue had elevated numbers of CD68+ (macrophage marker) cells and CD206+ (M2 macrophage marker) cells and increased CD47 expression. To explore potential interplay between colon cancer cells and M2 macrophages, we differentiated the monocyte cell line THP-1 into M1 and M2 macrophages (CD206high and Th2 cytokine-secreting cells), respe...
Source: European Journal of Cancer - June 28, 2013 Category: Cancer & Oncology Authors: Yuan Zhang, Wondossen Sime, Maria Juhas, Anita Sjölander Tags: Basic and preclinical research Source Type: research