Exosomes from mesenchymal stem cells expressing miR ‐125b inhibit neointimal hyperplasia via myosin IE
AbstractIntercellular communication between mesenchymal stem cells (MSCs) and their target cells in the perivascular environment is modulated by exosomes derived from MSCs. However, the potential role of exosome ‐mediated microRNA transfer in neointimal hyperplasia remains to be investigated. To evaluate the effects of MSC‐derived exosomes (MSC‐Exo) on neointimal hyperplasia, their effects upon vascular smooth muscle cell (VSMC) growth in vitro and neointimal hyperplasia in vivo were assessed in a m odel of balloon‐induced vascular injury. Our results showed that MSC‐Exo were internalised by VSMCs and i...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Dongqing Wang, Bin Gao, Jianing Yue, Fei Liu, Yifan Liu, Weiguo Fu, Yi Si Tags: ORIGINAL ARTICLE Source Type: research

O ‐GlcNAcylation promotes colorectal cancer progression by regulating protein stability and potential catcinogenic function of DDX5
This study not on ly reveals the novel functional of O‐GlcNAcylation in regulating DDX5, but also reveals the carcinogenic effect of the OGT‐DDX5 axis in colorectal cancer. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Nan Wu, Mingzuo Jiang, Yuying Han, Haiming Liu, Yi Chu, Hao Liu, Jiayi Cao, Qiuqiu Hou, Yu Zhao, Bing Xu, Xin Xie Tags: ORIGINAL ARTICLE Source Type: research

Identification of a 4 ‐mRNA metastasis‐related prognostic signature for patients with breast cancer
AbstractMetastasis ‐related mRNAs have showed great promise as prognostic biomarkers in various types of cancers. Therefore, we attempted to develop a metastasis‐associated gene signature to enhance prognostic prediction of breast cancer (BC) based on gene expression profiling. We firstly screened and identified 5 6 differentially expressed mRNAs by analysing BC tumour tissues with and without metastasis in the discovery cohort (GSE102484, n = 683). We then found 26 of these differentially expressed genes were associated with metastasis‐free survival (MFS) in the training set (GSE20685, n = 319). ...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Xinhua Xie, Jianwei Wang, Dingbo Shi, Yutian Zou, Zhenchong Xiong, Xing Li, Jianhua Zhou, Hailin Tang, Xiaoming Xie Tags: ORIGINAL ARTICLE Source Type: research

Disabled ‐1 is down‐regulated in clinical breast cancer and regulates cell apoptosis through NF‐κB/Bcl‐2/caspase‐9
In this study, we examined the expression of Dab1 in 38 breast cancer paraffin sections, as well as 60 paired frozen breast cancer and their adjacent tissues. Our results showed that Dab1 was reduced in breast cancer, and its compromised expression correlated with triple negative breast cancer phenotype, poor differentiation, as well as lymph node metastasis. Functional analysis in breast cancer cell lines demonstrated t hat Dab1 promoted cell apoptosis, which, at least partially, depended on its regulation of NF‐κB/Bcl‐2/caspase‐9 pathway. Our study strongly suggests thatDab1 may be a potential tumour suppress...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Rang ‐Juan Cao, Kai Li, Wan‐Ying Xing, Shuang Du, Qiang Li, Xiao‐Juan Zhu, Shu‐Sen Cui Tags: SHORT COMMUNICATION Source Type: research

One method to establish Epstein ‐Barr virus‐associated NK/T cell lymphoma mouse models
AbstractNovel nude mice model of human NK/T cell lymphoma were established by subcutaneously injecting two NK/T cell lymphoma cell lines into the right axillary region of mice and successful passages were completed by injecting cell suspension which was obtained through a 70 ‐μm cell strainer. These mice models and corresponding cell clones have been successfully developed for more than 8 generations. The survival rates of both resuscitation and transplantation in NKYS and YT models were 90% and 70% correspondingly. Pathologically, the tumour cells in all passages of the lymphoma‐bearing mice and cell lines obtained...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Weili Xue, Weiming Li, Yufeng Shang, Yanjie Zhang, Xuan Lan, Guannan Wang, Zhaoming Li, Xudong Zhang, Yue Song, Baopeng Wu, Meng Dong, Xinhua Wang, Mingzhi Zhang Tags: ORIGINAL ARTICLE Source Type: research

Preconditioning strategies for improving the survival rate and paracrine ability of mesenchymal stem cells in acute kidney injury
AbstractAcute kidney injury (AKI) is a common, severe emergency case in clinics, with high incidence, significant mortality and increased costs. Despite development in the understanding of its pathophysiology, the therapeutic choices are still confined to dialysis and renal transplantation. Considering their antiapoptotic, immunomodulatory, antioxidative and pro ‐angiogenic effects, mesenchymal stem cells (MSCs) may be a promising candidate for AKI management. Based on these findings, some clinical trials have been performed, but the results are contradictory (NCT00733876, NCT01602328). The low engraftment, poor survival...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Lingfei Zhao, Chenxia Hu, Ping Zhang, Hua Jiang, Jianghua Chen Tags: REVIEW Source Type: research

Up ‐regulation of SPINT2/HAI‐2 by Azacytidine in bone marrow mesenchymal stromal cells affects leukemic stem cell survival and adhesion
AbstractThe role of tumour microenvironment in neoplasm initiation and malignant evolution has been increasingly recognized. However, the bone marrow mesenchymal stromal cell (BMMSC) contribution to disease progression remains poorly explored. We previously reported that the expression of serine protease inhibitor kunitz ‐type2 (SPINT2/HAI‐2), an inhibitor of hepatocyte growth factor (HGF) activation, is significantly lower in BMMSC from myelodysplastic syndromes (MDS) patients compared to healthy donors (HD). Thus, to investigate whether this loss of expression was due to SPINT2/HAI‐2 methylation, BMMSC from MDS and...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Fernanda Marconi Roversi, Nathalia Moreno Cury, Matheus Rodrigues Lopes, Karla Priscila Ferro, Jo ão Agostinho Machado‐Neto, Marisa Claudia Alvarez, Gabriela Pereira Santos, Renata Giardini Rosa, Ana Leda Longhini, Adriana da Silva Santos Duar Tags: ORIGINAL ARTICLE Source Type: research

Protease ‐activated receptor‐1 contributes to renal injury and interstitial fibrosis during chronic obstructive nephropathy
AbstractEnd ‐stage renal disease, the final stage of all chronic kidney disorders, is associated with renal fibrosis and inevitably leads to renal failure and death. Transition of tubular epithelial cells (TECs) into mesenchymal fibroblasts constitutes a proposed mechanism underlying the progression of renal fibrosis and here we assessed whether protease‐activated receptor (PAR)‐1, which recently emerged as an inducer of epithelial‐to‐mesenchymal transition (EMT), aggravates renal fibrosis. We show that PAR‐1 activation on TECs reduces the expression of epithelial markers and simultaneously in duces mesenchymal...
Source: Journal of Cellular and Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Maaike Waasdorp, Dennis M. de Rooij, Sandrine Florquin, JanWillem Duitman, C. Arnold Spek Tags: ORIGINAL ARTICLE Source Type: research

Plasma levels of soluble ST2, but not IL ‐33, correlate with the severity of alcoholic liver disease
AbstractAlcoholic liver disease (ALD) is a complication that is a burden on global health and economy. Interleukin ‐33 (IL‐33) is a newly identified member of the IL‐1 cytokine family and is released as an “alarmin” during inflammation. Soluble suppression of tumourigenicity 2 (sST2), an IL‐33 decoy receptor, has been reported as a new biomarker for the severity of systemic and highly inflammatory di seases. Here, we found the levels of plasma sST2, increased with the disease severity from mild to severe ALD. Importantly, the plasma sST2 levels in ALD patients not only correlated with scores for prognos...
Source: Journal of Cellular and Molecular Medicine - November 27, 2018 Category: Molecular Biology Authors: Zijian Sun, Binxia Chang, Ang Huang, Shuli Hao, Miaomiao Gao, Ying Sun, Ming Shi, Lei Jin, Wei Zhang, Jun Zhao, Guangju Teng, Lin Han, Hui Tian, Qingsheng Liang, Ji ‐Yuan Zhang, Zhengsheng Zou Tags: ORIGINAL ARTICLE Source Type: research

CXXC5: A novel regulator and coordinator of TGF ‐β, BMP and Wnt signaling
AbstractCXXC5 is a member of the CXXC ‐type zinc‐finger protein family. Proteins in this family play a pivotal role in epigenetic regulation by binding to unmethylated CpG islands in gene promoters through their characteristic CXXC domain. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein‐protein interactions. Intriguingly, increasing evidence indicates that expression of the CXXC5 gene is controlled by multiple signaling pathways and a variety of transcription factors, positi...
Source: Journal of Cellular and Molecular Medicine - November 27, 2018 Category: Molecular Biology Authors: Xiangyang Xiong, Shuo Tu, Jianbin Wang, Shiwen Luo, Xiaohua Yan Tags: REVIEW Source Type: research

Therapeutic effects of lentinan on inflammatory bowel disease and colitis ‐associated cancer
In this study, we investigated the therapeutic potential of lentinan in mouse models of inflammatory bowel disease (IBD) and colitis ‐associated cancer (CAC). Lentinan decreased the disease activity index and macroscopic and microscopic colon tissue damage in dextran sulphate sodium (DSS)‐induced or TNBS‐induced models of colitis. High‐dose lentinan was more effective than salicylazosulfapyridine in the mouse models of co litis. Lentinan decreased the number of tumours, inflammatory cell infiltration, atypical hyperplasia and nuclear atypia in azoxymethane/DSS‐induced CAC model. It also decreased the expression o...
Source: Journal of Cellular and Molecular Medicine - November 24, 2018 Category: Molecular Biology Authors: Yanrong Liu, Jianmin Zhao, Yali Zhao, Shumin Zong, Yixuan Tian, Shuang Chen, Meng Li, Huijuan Liu, Qiang Zhang, Xueshuang Jing, Bo Sun, Hongzhi Wang, Tao Sun, Cheng Yang Tags: ORIGINAL ARTICLE Source Type: research

Plasma levels of miR ‐29b and miR‐200b in type 2 diabetic retinopathy
AbstractMicroRNAs (miRNAs/miRs) are involved in the pathogenesis of diabetes mellitus and its chronic complications, and their circulating levels have emerged as potential biomarkers for the development and progression of diabetes. However, few studies have examined the expression of miRNAs in diabetic retinopathy (DR) in humans. This case ‐control study aimed to investigate whether the plasma levels of miR‐29b and miR‐200b are associated with DR in 186 South Brazilians with type 2 diabetes (91 without DR, 46 with non‐proliferative DR and 49 with proliferative DR). We also included 20 healthy blood donors to determ...
Source: Journal of Cellular and Molecular Medicine - November 23, 2018 Category: Molecular Biology Authors: Maria Enoia Dantas da Costa e Silva, Evelise Regina Polina, Daisy Crispim, Renan Cesar Sbruzzi, Daniel Lavinsky, Felipe Mallmann, Nidiane Carla Martinelli, Luis Henrique Canani, Katia Gon çalves Santos Tags: ORIGINAL ARTICLE Source Type: research

Evaluation of serum exosomal LncRNA ‐based biomarker panel for diagnosis and recurrence prediction of bladder cancer
In this study, our aim was to identify a panel of lncRNAs in serum exosomes for the diagnosis and recurrence prediction of bladder cancer (BC). The expressions of 11 candidate lncRNAs in exosome were investigated in training set (n = 200) and an independent validation set (n = 320) via quantitative real‐time PCR. A three‐lncRNA panel (PCAT‐1, UBC1 and SNHG16) was finally identified by multivariate logistic regression model to provide high diagnostic accuracy for BC with an area under the receiver‐operating characteristic curve (AUC) of 0.857 and 0.826 in t raining set and validation set, respect...
Source: Journal of Cellular and Molecular Medicine - November 23, 2018 Category: Molecular Biology Authors: Shujun Zhang, Lutao Du, Lishui Wang, Xiumei Jiang, Yao Zhan, Juan Li, Keqiang Yan, Weili Duan, Yinghui Zhao, Lili Wang, Yunshan Wang, Yuliang Shi, Chuanxin Wang Tags: ORIGINAL ARTICLE Source Type: research

MiR ‐15a/16‐1 deficiency induces IL‐10‐producing CD19+ TIM‐1+ cells in tumor microenvironment
AbstractIL ‐10‐producing B cells (B10) are associated with autoimmune diseases, infection and tumours. MiR‐15a/16 as a tumour‐suppressive gene is down‐regulated in several tumours, such as chronic lymphocytic leukaemia, pituitary adenomas and prostate carcinoma. Here, increased frequency of IL‐10‐ producing CD19+ Tim ‐1+ cells was seen in both aged miR ‐15a/16−/− mice (15 ‐18 months) with the onset of B cell leukaemia and young knockout mice (8‐12 weeks) transplanted with hepatic cancer cells. CD19+ Tim ‐1+ cells down ‐regulated the function of effector CD4+CD25low T cells ex...
Source: Journal of Cellular and Molecular Medicine - November 23, 2018 Category: Molecular Biology Authors: Xiaoqin Jia, Hao Liu, Chong Xu, Sen Han, Yating Shen, Xin Miao, Xiangyu Hu, Zhijie Lin, Li Qian, Zhengbing Wang, Weijuan Gong Tags: ORIGINAL ARTICLE Source Type: research

ADIPOQ, KCNJ11 and TCF7L2 polymorphisms in type 2 diabetes in Kyrgyz population: A case ‐control study
This study demonstrated some association of 276T allele andADIPOQ gene G276T heterozygous genotype as well asKCNJ11 gene 23Lys allele with T2D in ethnic Kyrgyz, but study results should be interpreted with caution because of the limited statistical power. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 23, 2018 Category: Molecular Biology Authors: Jainagul Isakova, Elnura Talaibekova, Denis Vinnikov, Iskender Saadanov, Nazira Aldasheva Tags: SHORT COMMUNICATION Source Type: research

Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation
AbstractKey regulators of the Wnt signalling, DVL1, DVL2 and DVL3, in astrocytomas of different malignancy grades were investigated. Markers forDVL1, DVL2 andDVL3 were used to detect microsatellite instability (MSI) and gross deletions (LOH), while immunohistochemistry and immunoreactivity score were used to determine the signal strengths of the three DVL proteins and transcription factors of the pathway, TCF1 and LEF1. Our findings demonstrated that MSI at all threeDVL loci was constantly found across tumour grades with the highest number in grade II (P = 0.008). Collectively, LOHs were more frequent in high‐g...
Source: Journal of Cellular and Molecular Medicine - November 23, 2018 Category: Molecular Biology Authors: Anja Kafka, Mateja Ba čić, Davor Tomas, Kamelija Žarković, Anja Bukovac, Niko Njirić, Goran Mrak, Željka Krsnik, Nives Pećina‐Šlaus Tags: ORIGINAL ARTICLE Source Type: research

LncRNA MEG3 inhibits the progression of prostate cancer by modulating miR ‐9‐5p/QKI‐5 axis
This study was designed to detecting the influences of lncRNA MEG3 in prostate cancer. Aberrant lncRNAs expression profiles of prostate cancer were screened by microarray analysis. The qRT ‐PCR and Western blot were employed to investigating the expression levels of lncRNA MEG3, miR‐9‐5p andQKI ‐5. The luciferase reporter assay was utilized to testifying the interactions relationship among these molecules. Applying CCK ‐8 assay, wound healing assay, transwell assay and flow cytometry in turn, the cell proliferation, migration and invasion abilities as well as apoptosis were measured respectively. LncRNA MEG3 was ...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Meng Wu, Yawei Huang, Tongchang Chen, Weichao Wang, Shiguang Yang, Zhenfeng Ye, Xiaoqing Xi Tags: ORIGINAL ARTICLE Source Type: research

Gliomasphere marker combinatorics: multidimensional flow cytometry detects CD44+/CD133+/ITGA6+/CD36+ signature
AbstractGlioblastoma is the most dangerous brain cancer. One reason for glioblastoma's aggressiveness are glioblastoma stem ‐like cells. To target them, a number of markers have been proposed (CD133, CD44, CD15, A2B5, CD36, CXCR4, IL6R, L1CAM, and ITGA6). A comprehensive study of co‐expression patterns of them has, however, not been performed so far. Here, we mapped the multidimensional co‐expression profile of the se stemness‐associated molecules. Gliomaspheres – an established model of glioblastoma stem‐like cells – were used. Seven different gliomasphere systems were subjected to multicolor flow cy...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Friedrich Erhart, Bernadette Blauensteiner, Gabriel Zirkovits, Dieter Printz, Klara Soukup, Simone Klingenbrunner, Katrin Fischhuber, Ren é Reitermaier, Angela Halfmann, Daniela Lötsch, Sabine Spiegl‐Kreinecker, Walter Berger, Carmen Visus Tags: ORIGINAL ARTICLE Source Type: research

Testin protects against cardiac hypertrophy by targeting a calcineurin ‐dependent signalling pathway
This study investigated the influence of TES on cardiac hypertrophy and its etiology. Murine models with excessive TES expression specific to the heart were constructed with an adeno ‐associated virus expression system. Cardiac hypertrophy was stimulated through aortic banding (AB). The severity of cardiac hypertrophy was evaluated through molecular, echocardiographic, pathological, and hemodynamic examination. The findings of our study revealed that TES expression was remarka bly suppressed not only in failing human hearts but also in mouse hearts with cardiac hypertrophy. It was discovered that excessive TES expression...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Lu Gao, Yuan Liu, Sen Guo, Lili Xiao, Cui Liang, Xiaofang Wang Tags: ORIGINAL ARTICLE Source Type: research

MicroRNA let ‐7a regulates angiogenesis by targeting TGFBR3 mRNA
AbstractAngiogenesis has a great impact on human health, owing to its participation in development, wound healing and the pathogenesis of several diseases. It has been reported that let ‐7a is a tumour suppressor, but whether it plays a role in angiogenesis is unclear. Here we showed that let‐7a, a microRNA conserved in vertebrates, regulated angiogenesis by concomitantly down‐regulating TGFBR3. Overexpression of let‐7a or knockdown of TGFBR3 in cell culture inhibited the t ube formation and reduced migration rate. Moreover, xenograft experiments showed that overexpression of let‐7a or knockdown of TGFBR3 had sma...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Shao Wang, Huandong Zhou, Dazhou Wu, Huajing Ni, Zhongliang Chen, Chengshui Chen, Youqun Xiang, Kezhi Dai, Xiaoming Chen, Xi Li Tags: ORIGINAL ARTICLE Source Type: research

Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors
In this study, we extended our analysis of the molecular effects of romidepsin to deepen our understanding of the underlying mechanisms. P atients will benefit from these analyses, since detailed knowledge of the romidepsin effects allows for a better risk and side‐effect assessment. We screened for changes in histone acetylation of specific lysine residues and analysed changes in the DNA methylation landscape after romidepsin treatm ent of the GCT cell lines TCam‐2, 2102EP, NCCIT and JAR, while human fibroblasts were used as controls. In addition, we focused on the role of the dehydrogenase/reductaseDHRS2, which was s...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Daniel Nettersheim, Daniel Berger, Sina Jostes, Margaretha Skowron, Hubert Schorle Tags: ORIGINAL ARTICLE Source Type: research

The variant at TGFBRAP1 is significantly associated with type 2 diabetes mellitus and affects diabetes ‐related miRNA expression
AbstractWhile the transforming growth factor ‐β1 (TGF‐β1) regulates the growth and proliferation of pancreatic β‐cells, its receptors trigger the activation of Smad network and subsequently induce the insulin resistance. A case‐control was conducted to evaluate the associations of the polymorphisms of TGF‐β1 receptor‐associated protein 1 (TGFBRAP1) and TGF ‐β1 receptor 2 (TGFBR2) with type 2 diabetes mellitus (T2DM), and its genetic effects on diabetes ‐related miRNA expression. miRNA microarray chip was used to screen T2DM‐related miRNA and 15 differential expressed miRNAs were f...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Song Yang, Xiaotian Chen, Mengyao Yang, Xianghai Zhao, Yanchun Chen, Hailong Zhao, Chunlan Liu, Chong Shen Tags: ORIGINAL ARTICLE Source Type: research

Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling ‐dependent manner
In this study, we found that FGF21 was down ‐regulated in pancreatic islets of db/db mice, a mouse model of T2DM, along with decreased insulin expression, suggesting the possible involvement of FGF21 in maintaining insulin homeostasis and islet β‐cell function. Importantly, FGF21 knockout exacerbated palmitate‐induced islet β‐cell f ailure and suppression of glucose‐stimulated insulin secretion (GSIS). Pancreatic FGF21 overexpression significantly increased insulin expression, enhanced GSIS, improved islet morphology and reduced β‐cell apoptosis in db/db mice. Mechanistically, FGF21 promoted expr...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Yingying Pan, Baile Wang, Jujia Zheng, Rongrong Xiong, Zhichao Fan, Yanna Ye, Saisai Zhang, Qinyao Li, Fanghua Gong, Chaoming Wu, Zhuofeng Lin, Xiaokun Li, Xuebo Pan Tags: ORIGINAL ARTICLE Source Type: research

Novel peptide dermaseptin ‐PS1 exhibits anticancer activity via induction of intrinsic apoptosis signalling
In this study, a novel AMP named Dermaseptin ‐PS1 was isolated and identified fromPhyllomedusa sauvagei, it showed strong antimicrobial activities against three types of microorganisms. In vitro antiproliferative studies on human glioblastoma U ‐251 MG cells indicated that Dermaseptin‐PS1 disrupted cell membranes at the concentrations of 10−5 M and above, while the cell membrane integrity was not affected when concentrations were decreased to 10−6 M or lower. Further examinations revealed that, at the relatively low concentration (10−6 M), Dermaseptin‐PS1 induced apoptosis through m...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Qilin Long, Lei Li, Hao Wang, Miaoran Li, Lei Wang, Mei Zhou, Qiaozhu Su, Tianbao Chen, Yuxin Wu Tags: ORIGINAL ARTICLE Source Type: research

Targeting PRMT5/Akt signalling axis prevents human lung cancer cell growth
AbstractThe emerging evidence reveals that protein arginine methyltransferase 5 (PRMT5) is involved in regulation of tumour cell proliferation and cancer development. Nevertheless, the exact role of PRMT5 in human lung cancer cell proliferation and the underlying molecular mechanism remains largely obscure. Here, we showed that PRMT5 was highly expressed in human lung cancer cells and lung cancer tissues. Furthermore, we generated PRMT5 stable knockdown cell lines (A549 and H1299 cells) and explored the functions of PRMT5 in lung cancer cell proliferation. We found that the down ‐regulation of PRMT5 by shRNA or the inhib...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Shikui Zhang, Yaqiong Ma, Xiaoyan Hu, Yonghua Zheng, Xiaoke Chen Tags: ORIGINAL ARTICLE Source Type: research

Small molecule nAS ‐E targeting cAMP response element binding protein (CREB) and CREB‐binding protein interaction inhibits breast cancer bone metastasis
In this study, we tested the effect of nAS‐E on breast cancer cell proliferation, survival, migration as well as osteoclast formation and bone resorption in vitro for the first time. Our results demonstrated that nAS‐E inh ibited breast cancer cell proliferation, migration, survival and suppressed osteoclast differentiation as well as bone resorption through inhibiting CREB‐CBP interaction. In addition, the in vivo effect of nAS‐E in protecting against breast cancer‐induced osteolysis was evaluated. Our results indicated that nAS‐E could reverse bone loss induced by MDA‐MB‐231 tumour. These results suggest ...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Min Jiang, Yufei Yan, Kai Yang, Zhuochao Liu, Jin Qi, Hanbing Zhou, Niandong Qian, Qi Zhou, Tianqi Wang, Xing Xu, Xiangshu Xiao, Lianfu Deng Tags: ORIGINAL ARTICLE Source Type: research

Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
AbstractWe evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS ‐Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS‐Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS‐Ex than Sham. Myocyte diameter and inter...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: David R. A. Reyes, Mariana J. Gomes, Camila M. Rosa, Luana U. Pagan, Silmeia G. Zanati, Ricardo L. Damatto, Eder A. Rodrigues, Robson F. Carvalho, Ana A. H. Fernandes, Paula F. Martinez, Aline R. R. Lima, Marcelo D. M. Cezar, Luiz E. F. M. Car Tags: ORIGINAL ARTICLE Source Type: research

Autocrine VEGF signalling on M2 macrophages regulates PD ‐L1 expression for immunomodulation of T cells
In conclusion, our findings provide a novel biological basis to support the current successful strategy using combined VEGF/PD ‐1 signalling blockade in cancer therapy. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Yin ‐Siew Lai, Rika Wahyuningtyas, Shin‐Peir Aui, Ko‐Tung Chang Tags: ORIGINAL ARTICLE Source Type: research

Tongguan capsule ‐derived herb reduces susceptibility to atrial fibrillation by inhibiting left atrial fibrosis via modulating cardiac fibroblasts
In conclusion, the current study demonstrated that TGD reduces susceptibility to AF and improves LA conduction function in rats with p ost‐MI by inhibiting left atrial fibrosis and modulating CFs. Targeting the CF population may be a novel antiarrhythmic therapeutic approach. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Shiyu Ma, Jin Ma, Liheng Guo, Junqi Bai, Shuai Mao, Minzhou Zhang Tags: ORIGINAL ARTICLE Source Type: research

Age ‐related ultrastructural changes of the basement membrane in the mouse blood‐brain barrier
AbstractThe blood ‐brain barrier (BBB) is essential for a functional neurovascular unit. Most studies focused on the cells forming the BBB, but very few studied the basement membrane (BM) of brain capillaries in ageing. We used transmission electron microscopy and electron tomography to investigate the BM of the BB B in ageing C57BL/6J mice. The thickness of the BM of the BBB from 24‐month‐old mice was double as compared with that of 6‐month‐old mice (107 nm vs 56 nm). The aged BBB showed lipid droplets gathering within the BM which further increased its thickness (up to 572 nm) and altered its str...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Laura Cristina Ceafalan, Tudor Emanuel Fertig, Teodora Cristina Gheorghe, Mihail Eugen Hinescu, Bogdan Ovidiu Popescu, Jens Pahnke, Mihaela Gherghiceanu Tags: ORIGINAL ARTICLE Source Type: research

Evodiamine inhibits RANKL ‐induced osteoclastogenesis and prevents ovariectomy‐induced bone loss in mice
AbstractPostmenopausal osteoporosis (PMO) is a progressive bone disease characterized by the over ‐production and activation of osteoclasts in elderly women. In our study, we investigated the anti‐osteoclastogenic effect of evodiamine (EVO) in vivo and in vitro, as well as the underlying mechanism. By using an in vitro bone marrow macrophage (BMM)‐derived osteoclast culture system, we f ound that EVO inhibited osteoclast formation, hydroxyapatite resorption and receptor activator of NF‐κB ligand (RANKL)‐induced osteoclast marker gene and protein expression. Mechanistically, we found that EVO ...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Haiming Jin, Lingya Yao, Kai Chen, Yuhao Liu, Qingqing Wang, Ziyi Wang, Qian Liu, Zhen Cao, Jacob Kenny, Jennifer Tickner, Xiangyang Wang, Jiake Xu Tags: ORIGINAL ARTICLE Source Type: research

Otx2 enhances transdifferentiation of M üller cells‐derived retinal stem cells into photoreceptor‐like cells
In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target f or gene therapy of retinal degenerative diseases. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Yu Xiong, Hongpei Ji, Zhipeng You, Fei Yao, Rongrong Zhou, Weitao Song, Xiaobo Xia Tags: ORIGINAL ARTICLE Source Type: research

The signal pathway for the repressive effect of dipyridamole on myofibroblast transdifferentiation
Journal of Cellular and Molecular Medicine, EarlyView. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Qiong Yan, Keisuke Ina, Seiichi Chiba, Huixing Wei, Shuji Tatsukawa, Yoshihisa Fujikura Tags: SHORT COMMUNICATION Source Type: research

Unveiling Ga(III) phthalocyanine —a different photosensitizer in neuroblastoma cellular model
AbstractPhthalocyanines (Pc) and their metallated derivatives are strongly considered for photodynamic therapy (PDT) possessing unique properties as possible new photosensitizers (PS). We have used toxicological assessments, real ‐time monitoring of cellular impedance, and imagistic measurements for assessing the in vitro dark toxicity and PDT efficacy of Ga(III)‐Pc in SHSy5Y neuroblastoma cells. We have established the non‐toxic concentration range of Ga(III)‐Pc, a compound which shows a high intracellular accumula tion, with perinuclear distribution in confocal microscopy. By choosing Ga(III)Pc non‐toxic d...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Carolina Constantin, Andreea ‐Roxana Lupu, Tudor Emanuel Fertig, Mihaela Gherghiceanu, Sevinci Pop, Rodica‐Mariana Ion, Monica Neagu Tags: ORIGINAL ARTICLE Source Type: research

Metformin relieves neuropathic pain after spinal nerve ligation via autophagy flux stimulation
In this study, we investigated the therapeutic effects of metformin on pain relief after spinal nerve ligation (SNL) and its underlying mechanism of autophagy regulation. Behavioural analysis, histological assessment, expression of c ‐Fos and molecular biological changes, as well as ultrastructural features, were investigated. Our findings showed that the number of autophagosomes and expression of autophagy markers, such as LC3 and beclin1, were increased, while the autophagy substrate protein p62, as well as the ubiquitinated proteins, were accumulated in the ipsilateral spinal cord. However, metformin enhanced the expr...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Weidong Weng, Chenglun Yao, Keshav Poonit, Xijie Zhou, Chao Sun, Feng Zhang, Hede Yan Tags: ORIGINAL ARTICLE Source Type: research

TRPV4 regulates matrix stiffness and TGF β1‐induced epithelial‐mesenchymal transition
AbstractSubstrate stiffness (or rigidity) of the extracellular matrix has important functions in numerous pathophysiological processes including fibrosis. Emerging data support a role for both a mechanical signal, for example, matrix stiffness, and a biochemical signal, for example, transforming growth factor β1 (TGFβ1), in epithelial‐mesenchymal transition (EMT), a process critically involved in fibrosis. Here, we report evidence showing that transient receptor potential vanilloid 4 (TRPV4), a mechanosensitive channel, is the likely mediator of EMT in response to both TGFβ1 and matrix stiffness. Sp ecific...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Shweta Sharma, Rishov Goswami, David X. Zhang, Shaik O. Rahaman Tags: ORIGINAL ARTICLE Source Type: research

Two human MARs effectively increase transgene expression in transfected CHO cells
In conclusion, MAR‐3 and MAR‐7 gene c an promote the expression of transgene in transfected CHO cells, and its effect may be related to the increase of the number of copies. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Qin Li, Chun ‐peng Zhao, Yan Lin, Chao Song, Fang Wang, Tian‐yun Wang Tags: SHORT COMMUNICATION Source Type: research

High proportion of transient neonatal zinc deficiency causing alleles in the general population
AbstractLoss of function (LoF) mutations in the zinc transporter SLC30A2/ZnT2 result in impaired zinc secretion into breast milk consequently causing transient neonatal zinc deficiency (TNZD) in exclusively breastfed infants. However, the frequency of TNZD causing alleles in the general population is yet unknown. Herein, we investigated 115 missense SLC30A2/ZnT2 mutations from the ExAC database, equally distributed in the entire coding region, harboured in 668 alleles in 60  706 healthy individuals of diverse ethnicity. To estimate the frequency of LoF SLC30A2/ZnT2 mutations in the general population, we used bioinfor...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Yarden Golan, Adrian Lehvy, Guy Horev, Yehuda G. Assaraf Tags: ORIGINAL ARTICLE Source Type: research

Engineering of TIMP ‐3 as a LAP‐fusion protein for targeting to sites of inflammation
We describe here the engineering of TIMP‐3 using a novel drug‐delivery system known as the ‘LAP technology’. This involves creatin g therapeutic proteins in fusion with the latency‐associated peptide (LAP) from the cytokine TGF‐? to generate proteins that are biologically inactive until cleavage of the LAP to release the therapy. LAP‐TIMP‐3 was successfully expressed in mammalian cells and the presence of the LAP result ed in a 14‐fold increase in the quantity of recombinant TIMP‐3 produced. LAP‐TIMP‐3 was latent until release from the LAP by treatment with matrix metalloproteinase when it c...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Ben M. Alberts, Sandra M. Sacre, Peter G. Bush, Lisa M. Mullen Tags: SHORT COMMUNICATION Source Type: research

Intracellular reduction in ATP levels contributes to CYT997 ‐induced suppression of metastasis of head and neck squamous carcinoma
AbstractThe incidence rate of head and neck squamous cell carcinoma (HNSCC) has steadily increased over the past decade. However, treatment options for metastatic HNSCC are often limited and the 5 ‐year survival rate has remained static. Therefore, the development and assessment of more efficient but less toxic therapeutic strategies is an unmet need for treatment of more extensive HNSCC. Here, we report that CYT997, a novel microtubule‐disrupting agent, exerts strong activity in inhibiti ng HNSCC cell invasion and metastasis. The loss of invasion capacity by CYT997 was accompanied by an associated increase in cell adh...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Xiangdong Zhao, Liwei Lang, Leilei He, Lixia Gao, David Chyan, Yuanping Xiong, Honglin Li, Hong Peng, Yong Teng Tags: ORIGINAL ARTICLE Source Type: research

Autotaxin/lysophosphatidic acid signaling mediates obesity ‐related cardiomyopathy in mice and human subjects
In conclusion, present study uncovers the correlation between circulating autotaxin and cardiac parameters in mice and human patient, and provided solid evidence of the therapeutic application of autotaxin inhibitor in combating obesity‐related cardiomyopathy. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Jiakan Weng, Sheng Jiang, Lu Ding, Yi Xu, Xiongfei Zhu, Peifeng Jin Tags: ORIGINAL ARTICLE Source Type: research

Endogenous reduction of miR ‐185 accelerates cardiac function recovery in mice following myocardial infarction via targeting of cathepsin K
AbstractAngiogenesis is critical for re ‐establishing the blood supply to the surviving myocardium after myocardial infarction (MI) in patients with acute coronary syndrome (ACS). MicroRNAs are recognised as important epigenetic regulators of endothelial function. The aim of this study was to determine the roles of microRNAs in angiogen esis. Eighteen circulating microRNAs including miR‐185‐5p were differently expressed in plasma from patients with ACS by high‐throughput RNA sequencing. The expressional levels of miR‐185‐5p were dramatically reduced in hearts isolated from mice following MI and cultured human u...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Chuan ‐Chang Li, Xue‐Ting Qiu, Quan Sun, Ji‐Peng Zhou, Hui‐Jun Yang, Wan‐Zhou Wu, Ling‐Fang He, Can‐E Tang, Guo‐Gang Zhang, Yong‐Ping Bai Tags: ORIGINAL ARTICLE Source Type: research

Osterix promotes the migration and angiogenesis of breast cancer by upregulation of S100A4 expression
In this study, we demonstrated that OSX was highly expressed in metastatic breast cancer cells. Moreover, it could upregulate the expression of S100 calcium binding protein A4 (S100A4) and potentiate breast cancer cell migration and tumor angiogenesis in  vitro and in vivo. Importantly, inhibition of S100A4 impaired OSX‐induced cell migration and capillary‐like tube formation. Restored S100A4 expression rescued OSX‐short hairpin RNA‐suppressed cell migration and capillary‐like tube formation. Moreover, the expression levels of OSX and S1 00A4 correlated significantly in human breast tumors. Our study su...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Shuang Qu, Jiahui Wu, Qianyi Bao, Bing Yao, Rui Duan, Xiang Chen, Lingyun Li, Hongyan Yuan, Yucui Jin, Changyan Ma Tags: ORIGINAL ARTICLE Source Type: research

Efficient method for isolation of reticulocyte RNA from healthy individuals and hemolytic anaemia patients
AbstractDespite enormous progress and development of high ‐throughput methods in genome‐wide mRNA analyses, data on the erythroid transcriptome are still limited, even though they could be useful in medical diagnostics and personalized therapy as well as in research on normal and pathological erythroid maturation. Although obtaining normal and patholog ical reticulocyte transcriptome profiles should contribute greatly to our understanding of the molecular bases of terminal erythroid differentiation as well as the mechanisms of the hematological diseases, a basic limitation of these studies is the difficulty of efficien...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Micha ł Skulski, Rafał Bartoszewski, Michał Majkowski, Beata Machnicka, Kazimierz Kuliczkowski, Aleksander F. Sikorski, Dżamila M. Bogusławska Tags: ORIGINAL ARTICLE Source Type: research

Fibroblast growth factor 21 facilitates peripheral nerve regeneration through suppressing oxidative damage and autophagic cell death
AbstractSeeking for effective drugs which are beneficial to facilitating axonal regrowth and elongation after peripheral nerve injury (PNI) has gained extensive attention. Fibroblast growth factor 21 (FGF21) is a metabolic factor that regulates blood glucose and lipid homeostasis. However, there is little concern for the potential protective effect of FGF21 on nerve regeneration after PNI and revealing related molecular mechanisms. Here, we firstly found that exogenous FGF21 administration remarkably promoted functional and morphologic recovery in a rat model of sciatic crush injury, manifesting as persistently improved mo...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Yingfeng Lu, Rui Li, Junyi Zhu, Yanqing Wu, Duohui Li, Lupeng Dong, Yiyang Li, Xin Wen, Fangzheng Yu, Hongyu Zhang, Xiao Ni, Shenghu Du, Xiaokun Li, Jian Xiao, Jian Wang Tags: ORIGINAL ARTICLE Source Type: research

Pharmacological effects of berberine on mood disorders
AbstractBerberine, a natural isoquinoline alkaloid, is used in herbal medicine and has recently been shown to have efficacy in the treatment of mood disorders. Furthermore, berberine modulates neurotransmitters and their receptor systems within the central nervous system. However, the detailed mechanisms of its action remain unclear. This review summarizes the pharmacological effects of berberine on mood disorders. Therefore, it may be helpful for potential application in the treatment of mood disorders. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Jie Fan, Kun Zhang, Yang Jin, Bingjini Li, Shuohui Gao, Jiaming Zhu, Ranji Cui Tags: REVIEW Source Type: research

Metformin attenuates the effect of Staphylococcus aureus on airway tight junctions by increasing PKC ζ‐mediated phosphorylation of occludin
This study demonstrates that metformin improves TJ barrier function by promoting the abundance and assembly of full length occludin at the TJ and that this process involves phosphorylation of the protein via an AMPK‐PKCζ pathway. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Kameljit K. Kalsi, James P. Garnett, Wishwanath Patkee, Alexina Weekes, Mark E. Dockrell, Emma H. Baker, Deborah L. Baines Tags: ORIGINAL ARTICLE Source Type: research

Clinical association of metabolic syndrome, C ‐reactive protein and testosterone levels with clinically significant prostate cancer
AbstractRecently, the influence that metabolic syndrome (MetS), hormonal alterations and inflammation might have on prostate cancer (PCa) risk has been a subject of controversial debate. Herein, we aimed to investigate the association between MetS ‐components, C‐reactive protein (CRP) and testosterone levels, and the risk of clinically significant PCa (Sig‐PCa) at the time of prostate biopsy. For that, men scheduled for transrectal ultrasound guided biopsy of the prostate were studied. Clinical, laboratory parameters and criteria for Me tS characterization just before the biopsy were collected. A total of 524 patient...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Enrique G ómez‐Gómez, Julia Carrasco‐Valiente, Juan Pablo Campos‐Hernández, Ana Maria Blanca‐Pedregosa, Juan Manuel Jiménez‐Vacas, Jesus Ruiz‐García, Jose Valero‐Rosa, Raul Miguel Luque, María José Requena‐Tapia Tags: ORIGINAL ARTICLE Source Type: research

DLG5 suppresses breast cancer stem cell ‐like characteristics to restore tamoxifen sensitivity by inhibiting TAZ expression
AbstractTamoxifen (TAM) is a primary drug for treatment of estrogen receptor positive breast cancer. However, TAM resistance remains a serious threat to breast cancer patients and may be attributed to increased stemness of breast cancer. Here, we show that discs large homolog 5 (DLG5) expression is down ‐regulated in TAM‐resistant breast cancer and cells. DLG5 silencing decreased the sensitivity to TAM and increased the frequency and stemness of CD44+/CD24− breast cancer stem cells (BCSCs) and TAZ, a transducer of the Hippo pathway, expression in MCF7 cells while DLG5 overexpression had opposite effects. TAZ sile...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Jie Liu, Juan Li, Pingping Li, Yina Jiang, He Chen, Ruiqi Wang, Fang Cao, Peijun Liu Tags: ORIGINAL ARTICLE Source Type: research

Immunometabolism features of metabolic deregulation and  cancer
AbstractImmunometabolism is a branch dealing at the interface of immune functionalities and metabolic regulations. Considered as a bidirectional trafficking, metabolic contents and their precursors bring a considerable change in immune cells signal transductions which as a result affect the metabolic organs and states as an implication. Lipid metabolic ingredients form a major chunk of daily diet and have a proven contribution in immune cells induction, which then undergo metabolic pathway shuffling inside their ownself. Lipid metabolic states activate relevant metabolic pathways inside immune cells that in turn prime appr...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Xue Wang, Feng ‐Feng Ping, Sahar Bakht, Jingjing Ling, Waseem Hassan Tags: REVIEW Source Type: research