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Hyper ‐acetylation contributes to the sensitivity of chemo‐resistant prostate cancer cells to histone deacetylase inhibitor Trichostatin A
Abstract Therapeutic agents are urgently needed for treating metastatic castration‐refractory prostate cancer (mCRPC) that is unresponsive to androgen deprivation and chemotherapy. Our screening assays demonstrated that chemotherapy‐resistant prostate cancer (PCa) cells are more sensitive to HDAC inhibitors than paired sensitive PCa cells, as demonstrated by cell proliferation and apoptosis in vitro and in vivo. Kinetic study revealed that TSA‐induced apoptosis was significantly dependent on enhanced transcription and protein synthesis in an early stage, which subsequently caused ER stress and apoptosis. Ch...
Source: Journal of Cellular and Molecular Medicine - January 12, 2018 Category: Molecular Biology Authors: Qingqing Xu, Xiaofei Liu, Shiqin Zhu, Xuelei Hu, Huanmin Niu, Xiulei Zhang, Deyu Zhu, Effat Un Nesa, Keli Tian, Huiqing Yuan Tags: Original Article Source Type: research
An Aza resveratrol –chalcone derivative 6b protects mice against diabetic cardiomyopathy by alleviating inflammation and oxidative stress
This study aimed to investigate the potential protective effect of 6b on DCM and underlying mechanism. In H9c2 myocardial cells, 6b potently decreased high glucose (HG)‐induced cell fibrosis, hypertrophy and apoptosis, alleviating inflammatory response and oxidant stress. In STZ‐induced type 1 diabetic mice (STZ‐DM1), orally administration with 6b for 16 weeks significantly attenuated cardiac hypertrophy, apoptosis and fibrosis. The expression of inflammatory cytokines and oxidative stress biomarkers was also suppressed by 6b distinctly, without affecting blood glucose and body weight. The anti‐inflammatory and ant...
Source: Journal of Cellular and Molecular Medicine - January 12, 2018 Category: Molecular Biology Authors: Shengban You, Jianchang Qian, Chuchu Sun, Hailing Zhang, Shiju Ye, Taiwei Chen, Zheng Xu, Jingying Wang, Weijian Huang, Guang Liang Tags: Original Article Source Type: research
Association between single nucleotide polymorphisms of TPH1 and TPH2 genes, and depressive disorders
The objective of our study was to confirm the association between the presence of polymorphic variants of TPH1 and TPH2 genes, and the development of depressive disorders. Six polymorphisms were selected: c.804‐7C>A (rs10488682), c.‐1668T>A (rs623580), c.803+221C>A (rs1800532), c.‐173A>T (rs1799913)—TPH1, c.‐1449C>A (rs7963803), and c.‐844G>T (rs4570625)—TPH2. A total of 510 DNA samples (230 controls and 280 patients) were genotyped using TaqMan probes. Among the studied polymoorphisms, the G/G genotype and G allele of c.804‐7C>A—TPH1, the T/T homozygote of c.803+221C>A...
Source: Journal of Cellular and Molecular Medicine - January 5, 2018 Category: Molecular Biology Authors: Paulina Wigner, Piotr Czarny, Ewelina Synowiec, Micha ł Bijak, Katarzyna Białek, Monika Talarowska, Piotr Galecki, Janusz Szemraj, Tomasz Sliwinski Tags: Original Article Source Type: research
H3 relaxin inhibits the collagen synthesis via ROS ‐ and P2X7R‐mediated NLRP3 inflammasome activation in cardiac fibroblasts under high glucose
Abstract Excessive production of reactive oxygen species (ROS) and P2X7R activation induced by high glucose increases NLRP3 inflammasome activation, which contributes to the pathogenesis of diabetic cardiomyopathy. Although H3 relaxin has been shown to inhibit cardiac fibrosis induced by isoproterenol, the mechanism has not been well studied. Here, we demonstrated that high glucose (HG) induced the collagen synthesis by activation of the NLRP3 inflammasome, leading to caspase‐1 activation, interleukin‐1β (IL‐1β) and IL‐18 secretion in neonatal rat cardiac fibroblasts. Moreover, we used a high‐glucose mo...
Source: Journal of Cellular and Molecular Medicine - January 5, 2018 Category: Molecular Biology Authors: Xiaohui Zhang, Yu Fu, Hui Li, Li Shen, Qing Chang, Liya Pan, Siting Hong, Xinhua Yin Tags: Original Article Source Type: research
Emerging role of Twist1 in fibrotic diseases
Abstract Epithelial–mesenchymal transition (EMT) is a pathological process that occurs in a variety of diseases, including organ fibrosis. Twist1, a basic helix–loop–helix transcription factor, is involved in EMT and plays significant roles in various fibrotic diseases. Suppression of the EMT process represents a promising approach for the treatment of fibrotic diseases. In this review, we discuss the roles and the underlying molecular mechanisms of Twist1 in fibrotic diseases, including those affecting kidney, lung, skin, oral submucosa and other tissues. We aim at providing new insight into the pathogen...
Source: Journal of Cellular and Molecular Medicine - January 5, 2018 Category: Molecular Biology Authors: Xiaoxuan Ning, Kun Zhang, Qingfeng Wu, Minna Liu, Shiren Sun Tags: Review Source Type: research
Outcomes of endoscopic and microscopic transsphenoidal surgery on non ‐functioning pituitary adenomas: a systematic review and meta‐analysis
Abstract Both microscopic and endoscopic transsphenoidal surgery are effective approaches for nonfunctioning pituitary adenomas. The issue on the comparison of their efficacy and safety remains inconsistent. A thorough search of the literatures (PubMed, EMBASE, MEDLINE) were performed up to March 2017. Studies reporting outcomes of microscopic or endoscopic transsphenoidal surgery on nonfunctioning pituitary adenomas were included. A meta‐analysis was performed focusing on the early stage and long term outcomes. The final search yielded 19 eligible studies enrolling 3847 patients, 389 of them underwent microscopic approa...
Source: Journal of Cellular and Molecular Medicine - January 4, 2018 Category: Molecular Biology Authors: Shi ‐Yuan Yu, Qiu Du, Si‐Yuan Yao, Ke‐Nan Zhang, Jian Wang, Zhe Zhu, Xiao‐Bing Jiang Tags: Short Communication Source Type: research
Mammalian target of rapamycin inhibition attenuates myocardial ischaemia –reperfusion injury in hypertrophic heart
Abstract Pathological cardiac hypertrophy aggravated myocardial infarction and is causally related to autophagy dysfunction and increased oxidative stress. Rapamycin is an inhibitor of serine/threonine kinase mammalian target of rapamycin (mTOR) involved in the regulation of autophagy as well as oxidative/nitrative stress. Here, we demonstrated that rapamycin ameliorates myocardial ischaemia reperfusion injury by rescuing the defective cytoprotective mechanisms in hypertrophic heart. Our results showed that chronic rapamycin treatment markedly reduced the phosphorylated mTOR and ribosomal protein S6 expression, but not Akt...
Source: Journal of Cellular and Molecular Medicine - January 4, 2018 Category: Molecular Biology Authors: Lei ‐Lei Ma, Xin Ma, Fei‐Juan Kong, Jun‐Jie Guo, Hong‐Tao Shi, Jian‐Bing Zhu, Yun‐Zeng Zou, Jun‐Bo Ge Tags: Original Article Source Type: research
GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial –mesenchymal transition through the TGF‐β1/Smad2 signalling pathway
This study investigated the effects of Golgi membrane protein 73 (GP73) on the epithelial–mesenchymal transition (EMT) and on bladder cancer cell invasion and metastasis through the TGF‐β1/Smad2 signalling pathway. Paired bladder cancer and adjacent tissue samples (102) and normal bladder tissue samples (106) were obtained. Bladder cancer cell lines (T24, 5637, RT4, 253J and J82) were selected and assigned to blank, negative control (NC), TGF‐β, thrombospondin‐1 (TSP‐1), TGF‐β1+ TSP‐1, GP73‐siRNA‐1, GP73‐siRNA‐2, GP73‐siRNA‐1+ TSP‐1, GP73‐siRNA‐1+ pcDNA‐GP73, WT1‐siRNA...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Han ‐Jie Yang, Ge‐Liang Liu, Bo Liu, Tian Liu Tags: Original Article Source Type: research
Therapeutic benefits of CD90 ‐negative cardiac stromal cells in rats with a 30‐day chronic infarct
Abstract Cardiac stromal cells (CSCs) can be derived from explant cultures, and a subgroup of these cells is viewed as cardiac mesenchymal stem cells due to their expression of CD90. Here, we sought to determine the therapeutic potential of CD90‐positive and CD90‐negative CSCs in a rat model of chronic myocardial infarction. We obtain CD90‐positive and CD90‐negative fractions of CSCs from rat myocardial tissue explant cultures by magnetically activated cell sorting. In vitro, CD90‐negative CSCs outperform CD90‐positive CSCs in tube formation and cardiomyocyte functional assays. In rats with a 30‐day infarct, ...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Deliang Shen, Miaoda Shen, Hongxia Liang, Junnan Tang, Bo Wang, Chuang Liu, Peiwen Wang, Jianzeng Dong, Ling Li, Jinying Zhang, Thomas G. Caranasos Tags: Original Article Source Type: research
Increased free Zn2+ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn2+ ‐transporters in heart failure
Abstract Zn2+‐homoeostasis including free Zn2+ ([Zn2+]i) is regulated through Zn2+‐transporters and their comprehensive understanding may be important due to their contributions to cardiac dysfunction. Herein, we aimed to examine a possible role of Zn2+‐transporters in the development of heart failure (HF) via induction of ER stress. We first showed localizations of ZIP8, ZIP14 and ZnT8 to both sarcolemma and S(E)R in ventricular cardiomyocytes (H9c2 cells) using confocal together with calculated Pearson's coefficients. The expressions of ZIP14 and ZnT8 were significantly increased with decreased ZIP8 level in HF. Mo...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Yusuf Olgar, Aysegul Durak, Erkan Tuncay, Ceylan Verda Bitirim, Evren Ozcinar, Mustafa Bahadir Inan, Zeynep Tokcaer ‐Keskin, Kamil Can Akcali, Ahmet Ruchan Akar, Belma Turan Tags: Original Article Source Type: research
The rocky road to personalized medicine in acute myeloid leukaemia
Abstract Acute myeloid leukaemia (AML) is a malignant disorder of the myeloid blood lineage characterized by impaired differentiation and increased proliferation of hematopoietic precursor cells. Recent technological advances have led to an improved understanding of AML biology but also uncovered the enormous cytogenetic and molecular heterogeneity of the disease. Despite this heterogeneity, AML is mostly managed by a ‘one‐size‐fits‐all’ approach consisting of intensive, highly toxic induction and consolidation chemotherapy. These treatment protocols have remained largely unchanged for the past several de...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Bryan Brinda, Irum Khan, Brian Parkin, Heiko Konig Tags: Review Source Type: research
Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries ‐inducing Streptococcus mutans
Abstract Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lacobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lacobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expressio...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Reham Wasfi, Ola A. Abd El ‐Rahman, Mai M. Zafer, Hossam M. Ashour Tags: Original Article Source Type: research
Leucocyte and Platelet ‐rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine
Abstract The wound healing is a complex process wherein inflammation, proliferation and regeneration evolve according to a spatio‐temporal pattern from the activation of coagulation cascade to the formation of a plug clot including fibrin matrix, blood‐borne cells and cytokines/growth factors. Creating environments conducive to tissue repair, the haemoderivatives are commonly proposed for the treatment of hard‐to‐heal wounds. Here, we explored in vitro the intrinsic regenerative potentialities of a leucocyte‐ and platelet‐rich fibrin product, known as CPL‐MB, defining the stemness grade of cells sproutin...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Rosa Di Liddo, Thomas Bertalot, Alessio Borean, Ivan Pirola, Alberto Argentoni, Sandra Schrenk, Carola Cenzi, Stefano Capelli, Maria Teresa Conconi, Pier Paolo Parnigotto Tags: Original Article Source Type: research
European versus Asian differences for the associations between paraoxonase ‐1 genetic polymorphisms and susceptibility to type 2 diabetes mellitus
In this study, we performed a meta‐analysis to clarify the association of the two PON1 variants with T2DM risk. We carried out a systematic search of PubMed, Embase, CNKI and Wanfang databases for studies published before June 2017. The pooled odds ratios (ORs) for the association and their corresponding 95% confidence intervals (CIs) were calculated by a random‐ or fixed‐effect model. A total of 50 eligible studies, including 34 and 16 studies were identified for the PON1 Q192R (rs662) and L55M (rs854560) polymorphism, respectively. As for the PON1 Q192R polymorphism, the 192R allele was a susceptible factor of T2DM...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Jian ‐Quan Luo, Huan Ren, Mou‐Ze Liu, Ping‐Fei Fang, Da‐Xiong Xiang Tags: Original Article Source Type: research
The effect of a single injection of irinotecan on the development of enamel in the Wistar rats
Abstract Cancer is the second most frequent cause of death in children. Because the prognosis for childhood malignancies has improved, attention has now focused on long‐term consequences of cancer treatment. The immediate effects of chemotherapy on soft tissues have been well described; however, there is less information about long‐term effects of chemotherapy on the development of dental tissues. To test the association between the effect of chemotherapy on enamel development, we examined two groups of rats: one that had received an intraperitoneal dose of 200 mg/kg of irinotecan, whereas the other (control) group had...
Source: Journal of Cellular and Molecular Medicine - December 29, 2017 Category: Molecular Biology Authors: Sali Al ‐Ansari, Rozita Jalali, Ton Bronckers, Judith Raber‐Durlacher, Richard Logan, Jan Lange, Frederik Rozema Tags: Original Article Source Type: research
Hypothalamic POMC expression is required for peripheral insulin action on hepatic gluconeogenesis through regulating STAT3 in sepsis rats
Abstract Liver injury and dysregulated glucose homoeostasis are common manifestations during sepsis. Although plenty of studies reported insulin could protect against multiple organ injuries caused by critical infections among patients, little was known about the precise mechanism. We investigated whether liver inflammatory pathway and central neuropeptides were involved in the process. In sepsis rats, hepatic IKK/NF‐κB pathway and STAT3 were strongly activated, along with reduced body weight, blood glucose and suppressed hepatic gluconeogenesis (GNG). Peripheral insulin administration efficiently attenuated liver ...
Source: Journal of Cellular and Molecular Medicine - December 28, 2017 Category: Molecular Biology Authors: Bin Feng, Nannan Zhang, Kaipeng Duan, Bimin Shi Tags: Original Article Source Type: research
ALDH2 gene G487A polymorphism and coronary artery disease: a meta ‐analysis including 5644 participants
Abstract Several studies indicate the mitochondrial Aldehyde Dehydrogenase‐2 (ALDH2) gene G487A polymorphism may be correlated with coronary artery disease (CAD) susceptibility, but a clear consensus has yet to be reached. To elucidate the relationship between the ALDH2 gene G487A polymorphism and CAD within the Chinese population, a meta‐analysis of 5644 subjects from nine individual studies was performed. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals were assessed using random or fixed‐effect models depending the heterogeneity existence or not. Our meta‐analysis found a significant ass...
Source: Journal of Cellular and Molecular Medicine - December 26, 2017 Category: Molecular Biology Authors: Yan ‐yan Li, Hui Wang, Jing‐jing Wu, Hyun Jun Kim, Xin‐xing Yang, Hong‐yu Geng, Ge Gong Tags: Original Article Source Type: research
(Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - December 26, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Effect of lysyl oxidase (LOX) on corpus cavernous fibrosis caused by ischaemic priapism
Abstract Penile fibrosis caused by ischemic priapism (IP) adversely affects patients’ erectile function. We explored the role of lysyl oxidase (LOX) in rat and human penes after ischemic priapism (IP) to verify the effects of anti‐LOX in relieving penile fibrosis and preventing erectile dysfunction caused by IP in rats. Seventy‐two rats were randomly divided into six groups: control group, control + β‐aminopropionitrile (BAPN) group, 9 hrs group, 9 hrs + BAPN group, 24 hrs group, and 24 hrs + BAPN group. β‐aminopropionitrile (BAPN), a specific inhibitor of LOX, was administered in the drinking water...
Source: Journal of Cellular and Molecular Medicine - December 26, 2017 Category: Molecular Biology Authors: Liang Gao, Changjing Wu, Fudong Fu, Xuanhe You, Xue Ma, Feng Qin, Tao Li, Run Wang, Jiuhong Yuan Tags: Short Communication Source Type: research
Biological characteristics of human menstrual blood ‐derived endometrial stem cells
In this study, we confirmed that MenSCs reside in shedding endometrium, and documented that up to 3 days of storage at 4°C has little impact on MenSCs, while the age of the donor and the number of passages are negatively associated with proliferation capacity of MenSCs. Moreover, we found that MenSCs were actually immune‐privileged and projected no risk of tumour formation. Also, we documented a lung‐ and liver‐dominated, spleen‐ and kidney‐involved organic distribution profile of MenSC 3 days after intravenous transfer into mice. At last, we suggested that MenSCs may have potentially therapeutic effects on d...
Source: Journal of Cellular and Molecular Medicine - December 26, 2017 Category: Molecular Biology Authors: Yanli Liu, Rongcheng Niu, Fen Yang, Yan Yan, Shengying Liang, Yuliang Sun, Ping Shen, Juntang Lin Tags: Original Article Source Type: research
Monotropein promotes angiogenesis and inhibits oxidative stress ‐induced autophagy in endothelial progenitor cells to accelerate wound healing
This study revealed that Mtp is a potential therapeutic for endothelial injury‐related wounds. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - December 26, 2017 Category: Molecular Biology Authors: Chenggui Wang, Cong Mao, Yiting Lou, Jianxiang Xu, Qingqing Wang, Zengjie Zhang, Qian Tang, Xiaolei Zhang, Huazi Xu, Yongzeng Feng Tags: Original Article Source Type: research
Haematoma scavenging in intracerebral haemorrhage: from mechanisms to the clinic
Abstract The products of erythrocyte lyses, haemoglobin (Hb) and haem, are recognized as neurotoxins and the main contributors to delayed cerebral oedema and tissue damage after intracerebral haemorrhage (ICH). Finding a means to efficiently promote absorption of the haemolytic products (Hb and haem) around the bleeding area in the brain through stimulating the function of the body's own garbage cleaning system is a novel clinical challenge and critical for functional recovery after ICH. In this review, available information of the brain injury mechanisms underlying ICH and endogenous haematoma scavenging system is provide...
Source: Journal of Cellular and Molecular Medicine - December 26, 2017 Category: Molecular Biology Authors: Gaiqing Wang, Li Wang, Xin ‐gang Sun, Jiping Tang Tags: Review Source Type: research
Gallic acid attenuates calcium calmodulin ‐dependent kinase II‐induced apoptosis in spontaneously hypertensive rats
Abstract Hypertension causes cardiac hypertrophy and leads to heart failure. Apoptotic cells are common in hypertensive hearts. Ca2+/calmodulin‐dependent protein kinase II (CaMKII) is associated with apoptosis. We recently demonstrated that gallic acid reduces nitric oxide synthase inhibition‐induced hypertension. Gallic acid is a trihydroxybenzoic acid and has been shown to have beneficial effects, such as anti‐cancer, anti‐calcification and anti‐oxidant activity. The purpose of this study was to determine whether gallic acid regulates cardiac hypertrophy and apoptosis in essential hypertension. Gallic acid sign...
Source: Journal of Cellular and Molecular Medicine - December 20, 2017 Category: Molecular Biology Authors: Li Jin, Zhe Hao Piao, Chun Ping Liu, Simei Sun, Bin Liu, Gwi Ran Kim, Sin Young Choi, Yuhee Ryu, Hae Jin Kee, Myung Ho Jeong Tags: Original Article Source Type: research
Testosterone regulates the expression and functional activity of sphingosine ‐1‐phosphate receptors in the rat corpus cavernosum
This study is to determine testosterone (T) modulating the expression and functional activity of S1P receptors in corpus cavernosum (CC). Adult male Sprague‐Dawley rats were randomly divided into three groups: sham‐operated controls, surgical castration and T supplemented group. Serum S1P levels were detected by high‐performance liquid chromatography. The expression of S1P1‐3 receptors and sphingosine kinases was detected by real‐time RT‐PCR. In vitro organ bath contractility and in vivo intracavernous pressure (ICP) measurement were also performed. T deprivation significantly decreased ICP rise. Meanwhile...
Source: Journal of Cellular and Molecular Medicine - December 20, 2017 Category: Molecular Biology Authors: Jing Yin, Yu ‐ming Guo, Ping Chen, He Xiao, Xing‐huan Wang, Michael E DiSanto, Xin‐hua Zhang Tags: Original Article Source Type: research
Profile of common prostate cancer risk variants in an unscreened Romanian population
Abstract To find sequence variants affecting prostate cancer (PCA) susceptibility in an unscreened Romanian population we use a genome‐wide association study (GWAS). The study population included 990 unrelated pathologically confirmed PCA cases and 1034 male controls. DNA was genotyped using Illumina SNP arrays, and 24.295.558 variants were imputed using the 1000 Genomes data set. An association test was performed between the imputed markers and PCA. A systematic literature review for variants associated with PCA risk identified 115 unique variants that were tested in the Romanian sample set. Thirty of the previously rep...
Source: Journal of Cellular and Molecular Medicine - December 20, 2017 Category: Molecular Biology Authors: Paul D. Iordache, Dana Mates, Bjarni Gunnarsson, Hannes P. Eggertsson, Patrick Sulem, J úlíus Guðmundsson, Stefania Benónísdóttir, Irma Eva Csiki, Stefan Rascu, Daniel Radavoi, Radu Ursu, Catalin Staicu, Violeta Calota, Angelica Voinoiu, Mariana Jin Tags: Original Article Source Type: research
Combination of arsenic trioxide and Dasatinib: a new strategy to treat Philadelphia chromosome ‐positive acute lymphoblastic leukaemia
In this study, we investigate the impact of adding arsenic trioxide (ATO) on the action of Dasatinib, a second‐generation TKI, in Ph+ ALL. We show that ATO cooperates with Dasatinib in both TKI‐sensitive and resistant Ph+ ALL cell lines to increase apoptosis and we unravel the underlying mechanisms. Indeed, combining ATO and Dasatinib leads to severe cell apoptosis by activating the UPR apoptotic IRE1/JNK/PUMA axis, while neutralizing the UPR ATF4‐dependent anti‐apoptotic axis, activated by ATO alone. Additionally, ATO and Dasatinib in combination repress the expression of several genes, which we previously showed ...
Source: Journal of Cellular and Molecular Medicine - December 20, 2017 Category: Molecular Biology Authors: Tao Wang, Chunyan Cheng, Lijun Peng, Mengqing Gao, Mengping Xi, Sophie Rousseaux, Saadi Khochbin, Jin Wang, Jianqing Mi Tags: Original Article Source Type: research
The impact on high ‐grade serous ovarian cancer of obesity and lipid metabolism‐related gene expression patterns: the underestimated driving force affecting prognosis
Abstract To investigate whether specific obesity/metabolism‐related gene expression patterns affect the survival of patients with ovarian cancer. Clinical and genomic data of 590 samples from the high‐grade ovarian serous carcinoma (HGOSC) study of The Cancer Genome Atlas (TCGA) and 91 samples from the Australian Ovarian Cancer Study were downloaded from the International Cancer Genome Consortium (ICGC) portal. Clustering of mRNA microarray and reverse‐phase protein array (RPPA) data was performed with 83 consensus driver genes and 144 obesity and lipid metabolism‐related genes. Association between different cluste...
Source: Journal of Cellular and Molecular Medicine - December 20, 2017 Category: Molecular Biology Authors: Mauricio A. Cuello, Sumie Kato, Francisca Liberona Tags: Original Article Source Type: research
LncRNA ‐DANCR contributes to lung adenocarcinoma progression by sponging miR‐496 to modulate mTOR expression
In conclusion, this study showed that DANCR might be an oncogenic lncRNA that regulates mTOR expression through directly binding to miR‐496. DANCR may be regarded as a biomarker or therapeutic target for ADC. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - December 20, 2017 Category: Molecular Biology Authors: Qing ‐chun Lu, Zhuang‐hua Rui, Zhong‐liang Guo, Wang Xie, Shan Shan, Tao Ren Tags: Original Article Source Type: research
TAT ‐MTS‐MCM fusion proteins reduce MMA levels and improve mitochondrial activity and liver function in MCM‐deficient cells
In this study, we used heterologous MTSs of human, nuclear‐encoded mitochondrial proteins, to target the human MCM protein into the mitochondria. All fusion proteins reached the mitochondria and successfully underwent processing. Treatment of MMA patient fibroblasts with these fusion proteins restored mitochondrial activity such as ATP production, mitochondrial membrane potential and oxygen consumption, indicating the importance of mitochondrial function in this disease. Treatment with the fusion proteins enhanced cell viability and most importantly reduced MMA levels. Treatment also enhanced albumin and urea secretion i...
Source: Journal of Cellular and Molecular Medicine - December 19, 2017 Category: Molecular Biology Authors: Tal Erlich ‐Hadad, Rita Hadad, Anat Feldman, Hagar Greif, Michal Lictenstein, Haya Lorberboum‐Galski Tags: Original Article Source Type: research
Aerobic exercise training rescues protein quality control disruption on white skeletal muscle induced by chronic kidney disease in rats
Abstract We tested whether aerobic exercise training (AET) would modulate the skeletal muscle protein quality control (PQC) in a model of chronic kidney disease (CKD) in rats. Adult Wistar rats were evaluated in four groups: control (CS) or trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise (60 min., five times/wk for 2 months). We evaluated motor performance (tolerance to exercise on the treadmill and rotarod), cross‐sectional area (CSA), gene and protein levels related to the unfolded protein response (UPR), protein synthesis/survive a...
Source: Journal of Cellular and Molecular Medicine - December 19, 2017 Category: Molecular Biology Authors: Wilson Max Almeida Monteiro De Moraes, Pamella Ramona Moraes Souza, Nathalie Alves Paix ão, Luís Gustavo Oliveira Sousa, Daniel Araki Ribeiro, Andrea G. Marshall, Jonato Prestes, Maria Claudia Irigoyen, Patricia Chakur Brum, Alessandra Medeiros Tags: Original Article Source Type: research
Importance and regulation of adult stem cell migration
Abstract Cell migration is an essential process throughout the life of vertebrates, beginning during embryonic development and continuing throughout adulthood. Stem cells have an inherent ability to migrate, that is as important as their capacity for self‐renewal and differentiation, enabling them to maintain tissue homoeostasis and mediate repair and regeneration. Adult stem cells reside in specific tissue niches, where they remain in a quiescent state until called upon and activated by tissue environmental signals. Cell migration is a highly regulated process that involves the integration of intrinsic signals from the ...
Source: Journal of Cellular and Molecular Medicine - December 7, 2017 Category: Molecular Biology Authors: Beatriz Lucas, Laura M. P érez, Beatriz G. Gálvez Tags: Review Source Type: research
GIMDA: Graphlet interaction ‐based MiRNA‐disease association prediction
Abstract MicroRNAs (miRNAs) have been confirmed to be closely related to various human complex diseases by many experimental studies. It is necessary and valuable to develop powerful and effective computational models to predict potential associations between miRNAs and diseases. In this work, we presented a prediction model of Graphlet Interaction for MiRNA‐Disease Association prediction (GIMDA) by integrating the disease semantic similarity, miRNA functional similarity, Gaussian interaction profile kernel similarity and the experimentally confirmed miRNA‐disease associations. The related score of a miRNA to a disease...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Xing Chen, Na ‐Na Guan, Jian‐Qiang Li, Gui‐Ying Yan Tags: Original Article Source Type: research
Unique regulation of Na ‐glutamine cotransporter SN2/SNAT5 in rabbit intestinal crypt cells during chronic enteritis
Abstract The only Na‐nutrient cotransporter described in mammalian small intestinal crypt cells is SN2/SNAT5, which facilitates glutamine uptake. In a rabbit model of chronic intestinal inflammation, SN2 stimulation is secondary to an increase in affinity of the cotransporter for glutamine. However, the immune regulation of SN2 in the crypt cells during chronic intestinal inflammation is unknown. We sought to determine the mechanism of regulation of Na‐nutrient cotransporter SN2 by arachidonic acid metabolites in crypt cells. The small intestines of New Zealand white male rabbits were inflamed via inoculation with Eime...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Soudamani Singh, Subha Arthur, Uma Sundaram Tags: Original Article Source Type: research
CCAAT/enhancer ‐binding protein β overexpression alleviates myocardial remodelling by regulating angiotensin‐converting enzyme‐2 expression in diabetes
Abstract Diabetic cardiomyopathy, a major cardiac complication, contributes to heart remodelling and heart failure. Our previous study discovered that CCAAT/enhancer‐binding protein β (C/EBPβ), a transcription factor that belongs to a family of basic leucine zipper transcription factors, interacts with the angiotensin‐converting enzyme 2 (ACE2) promoter sequence in other disease models. Here, we aimed to determine the role of C/EBPβ in diabetes and whether ACE2 expression is regulated by C/EBPβ. A type 1 diabetic mouse model was generated by an intraperitoneal injection of streptozotocin. Diabetic m...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Yuanyuan Tie, Chungang Zhai, Ya Zhang, Xiaoteng Qin, Fangpu Yu, Hongxuan Li, MeiRong Shan, Cheng Zhang Tags: Original Article Source Type: research
Association between dopamine and somatostatin receptor expression and pharmacological response to somatostatin analogues in acromegaly
Abstract Acromegaly is a hormonal disorder resulting from excessive growth hormone (GH) secretion frequently produced by pituitary adenomas and consequent increase in insulin‐like growth factor 1 (IGF‐I). Elevated GH and IGF‐I levels result in a wide range of somatic, cardiovascular, endocrine, metabolic and gastrointestinal morbidities. Somatostatin analogues (SSAs) form the basis of medical therapy for acromegaly and are currently used as first‐line treatment or as second‐line therapy in patients undergoing unsuccessful surgery. However, a considerable percentage of patients do not respond to SSAs treatment. So...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Eva Venegas ‐Moreno, Mari C. Vazquez‐Borrego, Elena Dios, Noelia Gros‐Herguido, Alvaro Flores‐Martinez, Esther Rivero‐Cortés, Ainara Madrazo‐Atutxa, Miguel A. Japón, Raúl M. Luque, Justo P. Castaño, David A. Cano, Alfonso Soto‐Moreno Tags: Original Article Source Type: research
Functional assessment of the BMPR2 gene in lymphoblastoid cell lines from Graves ’ disease patients
In this study, we analysed the possible influence of the c.419‐43delT BMPR2 variant in patients with Graves’ disease (GD), in a molecular basis, focusing our efforts on possible alterations in the mRNA processing and synthesis. The molecular assessment of this variant in patients with GD would shed light on the association between the BMPR2 gene and the disease. The variant was detected in 18%, 55% and 10% of patients with pulmonary arterial hypertension, GD and in general population, respectively. Patients with GD fold change showed increased BMPR2 expression when matched against the controls, with a mean of 4.21&...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Guillermo Pousada, Mauro Lago ‐Docampo, Sonia Prado, Rubén Varela‐Calviño, Beatriz Mantiñán, Diana Valverde Tags: Original Article Source Type: research
Neuregulin ‐1 is essential for nerve plexus formation during cardiac maturation
In this study, we generated a novel nrg1 null allele targeting all known isoforms of nrg1 in zebrafish and examined cardiac structural and functional parameters throughout development. We found that zebrafish nrg1 mutants instead survived until young adult stages when they exhibited reduced survivorship. This coincided with structural and functional defects in the developing juvenile and young adult hearts, as demonstrated by reduced intracardiac myocardial density, cardiomyocyte cell number, swimming performance and dysregulated heartbeat. Interestingly, nrg1 mutant hearts were missing long axons on the ventricle surface ...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Daniel Brown, Leigh Ann Samsa, Cade Ito, Hong Ma, Karla Batres, Rima Arnaout, Li Qian, Jiandong Liu Tags: Short Communication Source Type: research
TPPU enhanced exercise ‐induced epoxyeicosatrienoic acid concentrations to exert cardioprotection in mice after myocardial infarction
Abstract Exercise training (ET) is a safe and efficacious therapeutic approach for myocardial infarction (MI). Given the numerous benefits of exercise, exercise‐induced mediators may be promising treatment targets for MI. C57BL/6 mice were fed 1‐trifluoromethoxyphenyl‐3‐(1‐propionylpiperidine‐4‐yl) urea (TPPU), a novel soluble epoxide hydrolase inhibitor (sEHI), to increase epoxyeicosatrienoic acid (EET) levels, for 1 week before undergoing MI surgery. After 1‐week recovery, the mice followed a prescribed exercise programme. Bone marrow‐derived endothelial progenitor cells (EPCs) were isolated from t...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Yuan Guo, Fei Luo, Xv Zhang, Jingyuan Chen, Li Shen, Yi Zhu, Danyan Xu Tags: Original Article Source Type: research
Relative abundance of mature myostatin rather than total myostatin is negatively associated with bone mineral density in Chinese
Abstract Myostatin is mainly secreted by skeletal muscle and negatively regulates skeletal muscle growth. However, the roles of myostatin on bone metabolism are still largely unknown. Here, we recruited two large populations containing 6308 elderly Chinese and conducted comprehensive statistical analyses to evaluate the associations among lean body mass (LBM), plasma myostatin, and bone mineral density (BMD). Our data revealed that total myostatin in plasma was mainly determined by LBM. The relative abundance of mature myostatin (mature/total) was significantly lower in high versus low BMD subjects. Moreover, the relative ...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Long ‐Fei Wu, Dong‐Cheng Zhu, Bing‐Hua Wang, Yi‐Hua Lu, Pei He, Yun‐Hong Zhang, Hong‐Qin Gao, Xiao‐Wei Zhu, Wei Xia, Hong Zhu, Xing‐Bo Mo, Xin Lu, Lei Zhang, Yong‐Hong Zhang, Fei‐Yan Deng, Shu‐Feng Lei Tags: Original Article Source Type: research
SERPINH1 overexpression in clear cell renal cell carcinoma: association with poor clinical outcome and its potential as a novel prognostic marker
In this study, we performed integrated proteomic and transcriptomic screening in all four tumour‐node‐metastasis stages of ccRCC and adjacent normal tissues (n = 18) to investigate differentially expressed genes. Most identified differentially expressed genes revealed a strong association with transforming growth factor‐β level and the epithelial‐to‐mesenchymal transition process. Of them, Serpin peptidase inhibitor clade H member 1 (SERPINH1) revealed the strongest association with poor prognosis and regulation on the expression levels of epithelial‐to‐mesenchymal transition markers. Subsequen...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Yijun Qi, Yue Zhang, Zhiqiang Peng, Lei Wang, Kaizhen Wang, Duiping Feng, Junqi He, Junfang Zheng Tags: Original Article Source Type: research
Monocytic myeloid ‐derived suppressor cells as prognostic factor in chronic myeloid leukaemia patients treated with dasatinib
In this study, we found that therapy with tyrosine kinase inhibitors (TKI) decreased the percentage of granulocytic MDSC, but only patients treated with dasatinib showed a significant reduction in the monocytic subset (M‐MDSC). Moreover, a positive correlation was observed between number of persistent M‐MDSC and the value of major molecular response in dasatinib‐treated patients. Serum and exosomes from patients with CML induced conversion of monocytes from healthy volunteers into immunosuppressive M‐MDSC, suggesting a bidirectional crosstalk between CML cells and MDSC. Overall, we identified M‐MDSC as prognostic...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Cesarina Giallongo, Nunziatina L. Parrinello, Piera La Cava, Giuseppina Camiolo, Alessandra Romano, Marina Scalia, Fabio Stagno, Giuseppe A. Palumbo, Roberto Avola, Giovanni Li Volti, Daniele Tibullo, Francesco Di Raimondo Tags: Original Article Source Type: research
CME ‐1, a novel polysaccharide, suppresses iNOS expression in lipopolysaccharide‐stimulated macrophages through ceramide‐initiated protein phosphatase 2A activation
In conclusion, CME‐1 suppressed iNOS expression by up‐regulating ceramide‐induced PP2A activation and reducing ROS production in LPS‐stimulated macrophages. CME‐1 is a potential therapeutic agent for treating inflammatory diseases. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Joen ‐Rong Sheu, Zhih‐Cherng Chen, Ming‐Jen Hsu, Shwu‐Huey Wang, Kuo‐Wei Jung, Wei‐Fan Wu, Szu‐Han Pan, Ruei‐Dun Teng, Chih‐Hao Yang, Cheng‐Ying Hsieh Tags: Original Article Source Type: research
Acute exposure to high ‐induction electromagnetic field affects activity of model peripheral sensory neurons
Abstract Exposure to repetitive low‐frequency electromagnetic field (LF‐EMF) shows promise as a non‐invasive approach to treat various sensory and neurological disorders. Despite considerable progress in the development of modern stimulation devices, there is a limited understanding of the mechanisms underlying their biological effects and potential targets at the cellular level. A significant impact of electromagnetic field on voltage‐gated calcium channels and downstream signalling pathways has been convincingly demonstrated in many distinct cell types. However, evidence for clear effects on primary sensory neuro...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Jaroslav Prucha, Jan Krusek, Ivan Dittert, Viktor Sinica, Anna Kadkova, Viktorie Vlachova Tags: Short Communication Source Type: research
Site ‐specific and endothelial‐mediated dysfunction of the alveolar‐capillary barrier in response to lipopolysaccharides
In this study, we analyse the site‐specific effects of LPS on the ACB and reveal the effects on the individual cell types and the ACB as a functional unit. Monocultures of H441 epithelial cells and co‐cultures of H441 with endothelial cells cultured on Transwells® were treated with LPS from the apical or basolateral compartment. Barrier properties were analysed by the transepithelial electrical resistance (TEER), by transport assays, and immunostaining and assessment of tight junctional molecules at protein level. Furthermore, pro‐inflammatory cytokines and immune‐modulatory molecules were evaluated by ELISA an...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Harshavardhan Janga, Liam Cassidy, Fanlu Wang, Dietmar Spengler, Stefanie Oestern ‐Fitschen, Martin F. Krause, Andreas Seekamp, Andreas Tholey, Sabine Fuchs Tags: Original Article Source Type: research
Neuroprotection by quercetin via mitochondrial function adaptation in traumatic brain injury: PGC ‐1α pathway as a potential mechanism
Abstract The aim of this study was to investigate the neuroprotective effects of quercetin in mouse models of traumatic brain injury (TBI) and the potential role of the PGC‐1α pathway in putative neuroprotection. Wild‐type mice were randomly assigned to four groups: the sham group, the TBI group, the TBI+vehicle group and the TBI+quercetin group. Quercetin, a dietary flavonoid used as a food supplement, significantly reduced TBI‐induced neuronal apoptosis and ameliorated mitochondrial lesions. It significantly accelerated the translocation of PGC‐1α protein from the cytoplasm to the nucleus. In addition...
Source: Journal of Cellular and Molecular Medicine - December 1, 2017 Category: Molecular Biology Authors: Xiang Li, Handong Wang, Guodao Wen, Liwen Li, Yongyue Gao, Zong Zhuang, Mengliang Zhou, Lei Mao, Youwu Fan Tags: Original Article Source Type: research
Physical forces modulate cell differentiation and proliferation processes
Abstract Currently, the predominant hypothesis explains cellular differentiation and behaviour as an essentially genetically driven intracellular process, suggesting a gene‐centrism paradigm. However, although many living species genetic has now been described, there is still a large gap between the genetic information interpretation and cell behaviour prediction. Indeed, the physical mechanisms underlying the cell differentiation and proliferation, which are now known or suspected to guide such as the flow of energy through cells and tissues, have been often overlooked. We thus here propose a complementary conceptual fr...
Source: Journal of Cellular and Molecular Medicine - November 30, 2017 Category: Molecular Biology Authors: Laurent Schwartz, Jorgelindo Veiga Moreira, Mario Jolicoeur Tags: Review Source Type: research
Identification of a novel compound targeting the nuclear export of influenza A virus nucleoprotein
In this study, we identified a novel anti‐influenza compound, ZBMD‐1, from a library of 20,000 compounds using cell‐based influenza A infection assays. We found that ZBMD‐1 inhibited the replication of H1N1 and H3N2 influenza A virus strains in vitro, with an IC50 ranging from 0.41–1.14 μM. Furthermore, ZBMD‐1 inhibited the polymerase activity and specifically impaired the nuclear export of NP. Further investigation indicated that ZBMD‐1 binds to the nuclear export signal 3 (NES3) domain and the dimer interface of the NP pocket. ZBMD‐1 also protected mice that were challenged with lethal do...
Source: Journal of Cellular and Molecular Medicine - November 30, 2017 Category: Molecular Biology Authors: Feng Huang, Jingliang Chen, Junsong Zhang, Likai Tan, Gui Lu, Yongjie Luo, Ting Pan, Juanran Liang, Qianwen Li, Baohong Luo, Hui Zhang, Gen Lu Tags: Original Article Source Type: research
Acute inhibition of PMCA4, but not global ablation, reduces blood pressure and arterial contractility via a nNOS ‐dependent mechanism
Abstract Cardiovascular disease is the world's leading cause of morbidity and mortality, with high blood pressure (BP) contributing to increased severity and number of adverse outcomes. Plasma membrane calcium ATPase 4 (PMCA4) has been previously shown to modulate systemic BP. However, published data are conflicting, with both overexpression and inhibition of PMCA4 in vivo shown to increase arterial contractility. Hence, our objective was to determine the role of PMCA4 in the regulation of BP and to further understand how PMCA4 functionally regulates BP using a novel specific inhibitor to PMCA4, aurintricarboxylic aci...
Source: Journal of Cellular and Molecular Medicine - November 30, 2017 Category: Molecular Biology Authors: Sophronia Lewis, Robert Little, Florence Baudoin, Sukhpal Prehar, Ludwig Neyses, Elizabeth J. Cartwright, Clare Austin Tags: Original Article Source Type: research
Identification of a novel RPGRIP1 mutation in an Iranian family with leber congenital amaurosis by exome sequencing
This study provides compelling evidence that the c. 2889delT (p.P963 fs) mutation in the RPGRIP1 gene works as a pathogenic mutation that contributes to the progression of LCA. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 29, 2017 Category: Molecular Biology Authors: Saber Imani, Jingliang Cheng, Abdolkarim Mobasher ‐Jannat, Chunli Wei, Shangyi Fu, Lisha Yang, Khosrow Jadidi, Mohammad Hossein Khosravi, Saman Mohazzab‐Torabi, Marzieh Dehghan Shasaltaneh, Yumei Li, Rui Chen, Junjiang Fu Tags: Original Article Source Type: research
(Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 29, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research