Proteomic Profiling of Muscular Adaptations to Short-term Concentric vs. Eccentric Exercise Training in Humans
In conclusion, the four-week concentric- and eccentric training resulted in distinctly different proteomic profiles, especially in proteins related to muscular structure and metabolism.PMID:38493954 | DOI:10.1016/j.mcpro.2024.100748 (Source: Molecular and Cellular Proteomics : MCP)
Source: Molecular and Cellular Proteomics : MCP - March 17, 2024 Category: Molecular Biology Authors: Jiawei Du Hezhang Yun Hongsheng Wang Xin Bai Yuhui Su Xiaochuan Ge Yang Wang Boya Gu Li Zhao Ji-Guo Yu Yafeng Song Source Type: research

Galectin-4 antimicrobial activity primarily occurs through its C-terminal domain
In this study, we demonstrate that the innate immune lectin, galectin-4 (Gal-4), exhibits strain-specific binding and killing behavior towards microbes that display blood group-like antigens. Examination of binding preferences using a combination of microarrays populated with ABO(H) glycans and a variety of microbial strains, including those that express blood group-like antigens, demonstrated that Gal-4 binds mammalian and microbial antigens that have features of blood group and mammalian-like structures. Although Gal-4 was thought to exist as a monomer that achieves functional bivalency through its two linked carbohydrat...
Source: Molecular and Cellular Proteomics : MCP - March 15, 2024 Category: Molecular Biology Authors: Hau-Ming Jan Shang-Chuen Wu Carter J Stowell Mary L Vallecillo-Z Ășniga Anu Paul Kashyap R Patel Sasikala Muthusamy Hsien-Ya Lin Diyoly Ayona Ryan Philip Jajosky Samata P Varadkar Hirotomo Nakahara Rita Chan Devika Bhave William J Lane Melissa Yeung Marie Source Type: research

Galectin-4 antimicrobial activity primarily occurs through its C-terminal domain
In this study, we demonstrate that the innate immune lectin, galectin-4 (Gal-4), exhibits strain-specific binding and killing behavior towards microbes that display blood group-like antigens. Examination of binding preferences using a combination of microarrays populated with ABO(H) glycans and a variety of microbial strains, including those that express blood group-like antigens, demonstrated that Gal-4 binds mammalian and microbial antigens that have features of blood group and mammalian-like structures. Although Gal-4 was thought to exist as a monomer that achieves functional bivalency through its two linked carbohydrat...
Source: Molecular and Cellular Proteomics : MCP - March 15, 2024 Category: Molecular Biology Authors: Hau-Ming Jan Shang-Chuen Wu Carter J Stowell Mary L Vallecillo-Z Ășniga Anu Paul Kashyap R Patel Sasikala Muthusamy Hsien-Ya Lin Diyoly Ayona Ryan Philip Jajosky Samata P Varadkar Hirotomo Nakahara Rita Chan Devika Bhave William J Lane Melissa Yeung Marie Source Type: research

Universal pretreatment development for low-input proteomics using lauryl maltose neopentyl glycol
In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMNG during sample preparation for general proteomic analysis is the prevention of mass spectrometry (MS) contamination. When we applied the LASP method to the low-input SP3 method and on-bead digestion in co-immunoprecipitation-MS, we observed a significant improvemen...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Ryo Konno Masaki Ishikawa Daisuke Nakajima Yusuke Endo Osamu Ohara Yusuke Kawashima Source Type: research

Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
Mol Cell Proteomics. 2024 Mar 4:100746. doi: 10.1016/j.mcpro.2024.100746. Online ahead of print.ABSTRACTHuntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive, and choreiform movements. Multiple lines of evidence point to an association between HD and aberrant striatal mitochondrial functions; however, the present knowledge about whether (or how) mitochondrial mRNA translation is differentially regulated in HD remains unclear. We found that protein synthesis is diminished in HD mitochondria compared t...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Sunayana Dagar Manish Sharma George Tsaprailis Catherina Scharager Tapia Gogce Crynen Preksha Sandipkumar Joshi Neelam Shahani Srinivasa Subramaniam Source Type: research

Universal pretreatment development for low-input proteomics using lauryl maltose neopentyl glycol
In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMNG during sample preparation for general proteomic analysis is the prevention of mass spectrometry (MS) contamination. When we applied the LASP method to the low-input SP3 method and on-bead digestion in co-immunoprecipitation-MS, we observed a significant improvemen...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Ryo Konno Masaki Ishikawa Daisuke Nakajima Yusuke Endo Osamu Ohara Yusuke Kawashima Source Type: research

Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
Mol Cell Proteomics. 2024 Mar 4:100746. doi: 10.1016/j.mcpro.2024.100746. Online ahead of print.ABSTRACTHuntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive, and choreiform movements. Multiple lines of evidence point to an association between HD and aberrant striatal mitochondrial functions; however, the present knowledge about whether (or how) mitochondrial mRNA translation is differentially regulated in HD remains unclear. We found that protein synthesis is diminished in HD mitochondria compared t...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Sunayana Dagar Manish Sharma George Tsaprailis Catherina Scharager Tapia Gogce Crynen Preksha Sandipkumar Joshi Neelam Shahani Srinivasa Subramaniam Source Type: research

Universal pretreatment development for low-input proteomics using lauryl maltose neopentyl glycol
In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMNG during sample preparation for general proteomic analysis is the prevention of mass spectrometry (MS) contamination. When we applied the LASP method to the low-input SP3 method and on-bead digestion in co-immunoprecipitation-MS, we observed a significant improvemen...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Ryo Konno Masaki Ishikawa Daisuke Nakajima Yusuke Endo Osamu Ohara Yusuke Kawashima Source Type: research

Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
Mol Cell Proteomics. 2024 Mar 4:100746. doi: 10.1016/j.mcpro.2024.100746. Online ahead of print.ABSTRACTHuntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive, and choreiform movements. Multiple lines of evidence point to an association between HD and aberrant striatal mitochondrial functions; however, the present knowledge about whether (or how) mitochondrial mRNA translation is differentially regulated in HD remains unclear. We found that protein synthesis is diminished in HD mitochondria compared t...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Sunayana Dagar Manish Sharma George Tsaprailis Catherina Scharager Tapia Gogce Crynen Preksha Sandipkumar Joshi Neelam Shahani Srinivasa Subramaniam Source Type: research

Universal pretreatment development for low-input proteomics using lauryl maltose neopentyl glycol
In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMNG during sample preparation for general proteomic analysis is the prevention of mass spectrometry (MS) contamination. When we applied the LASP method to the low-input SP3 method and on-bead digestion in co-immunoprecipitation-MS, we observed a significant improvemen...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Ryo Konno Masaki Ishikawa Daisuke Nakajima Yusuke Endo Osamu Ohara Yusuke Kawashima Source Type: research

Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
Mol Cell Proteomics. 2024 Mar 4:100746. doi: 10.1016/j.mcpro.2024.100746. Online ahead of print.ABSTRACTHuntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive, and choreiform movements. Multiple lines of evidence point to an association between HD and aberrant striatal mitochondrial functions; however, the present knowledge about whether (or how) mitochondrial mRNA translation is differentially regulated in HD remains unclear. We found that protein synthesis is diminished in HD mitochondria compared t...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Sunayana Dagar Manish Sharma George Tsaprailis Catherina Scharager Tapia Gogce Crynen Preksha Sandipkumar Joshi Neelam Shahani Srinivasa Subramaniam Source Type: research

Universal pretreatment development for low-input proteomics using lauryl maltose neopentyl glycol
In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMNG during sample preparation for general proteomic analysis is the prevention of mass spectrometry (MS) contamination. When we applied the LASP method to the low-input SP3 method and on-bead digestion in co-immunoprecipitation-MS, we observed a significant improvemen...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Ryo Konno Masaki Ishikawa Daisuke Nakajima Yusuke Endo Osamu Ohara Yusuke Kawashima Source Type: research

Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
Mol Cell Proteomics. 2024 Mar 4:100746. doi: 10.1016/j.mcpro.2024.100746. Online ahead of print.ABSTRACTHuntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive, and choreiform movements. Multiple lines of evidence point to an association between HD and aberrant striatal mitochondrial functions; however, the present knowledge about whether (or how) mitochondrial mRNA translation is differentially regulated in HD remains unclear. We found that protein synthesis is diminished in HD mitochondria compared t...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Sunayana Dagar Manish Sharma George Tsaprailis Catherina Scharager Tapia Gogce Crynen Preksha Sandipkumar Joshi Neelam Shahani Srinivasa Subramaniam Source Type: research

Universal pretreatment development for low-input proteomics using lauryl maltose neopentyl glycol
In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMNG during sample preparation for general proteomic analysis is the prevention of mass spectrometry (MS) contamination. When we applied the LASP method to the low-input SP3 method and on-bead digestion in co-immunoprecipitation-MS, we observed a significant improvemen...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Ryo Konno Masaki Ishikawa Daisuke Nakajima Yusuke Endo Osamu Ohara Yusuke Kawashima Source Type: research

Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease
Mol Cell Proteomics. 2024 Mar 4:100746. doi: 10.1016/j.mcpro.2024.100746. Online ahead of print.ABSTRACTHuntington disease (HD) is caused by an expanded polyglutamine mutation in huntingtin (mHTT) that promotes prominent atrophy in the striatum and subsequent psychiatric, cognitive, and choreiform movements. Multiple lines of evidence point to an association between HD and aberrant striatal mitochondrial functions; however, the present knowledge about whether (or how) mitochondrial mRNA translation is differentially regulated in HD remains unclear. We found that protein synthesis is diminished in HD mitochondria compared t...
Source: Molecular and Cellular Proteomics : MCP - March 6, 2024 Category: Molecular Biology Authors: Sunayana Dagar Manish Sharma George Tsaprailis Catherina Scharager Tapia Gogce Crynen Preksha Sandipkumar Joshi Neelam Shahani Srinivasa Subramaniam Source Type: research