Molecular and Cellular Proteomics : MCP This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

FMRP long-range transport and degradation are mediated by Dynlrb1 in sensory neurons
Mol Cell Proteomics. 2023 Sep 20:100653. doi: 10.1016/j.mcpro.2023.100653. Online ahead of print.ABSTRACTThe fragile X messenger ribonucleoprotein 1 (FMRP) is a multifunctional RNA binding protein (RBP) implicated in human neurodevelopmental and neurodegenerative disorders. FMRP mediates the localization and activity-dependent translation of its associated mRNAs through the formation of phase separated condensates that are trafficked by microtubule-based motors in axons. Axonal transport and localized mRNA translation are critical processes for long-term neuronal survival and are closely linked to the pathogenesis of neuro...
Source: Molecular and Cellular Proteomics : MCP - September 22, 2023 Category: Molecular Biology Authors: Sara Emad El-Agamy Laurent Guillaud Keiko Kono Yibo Wu Marco Terenzio Source Type: research

Clinical Proteomics for Solid Organ Tissues
Mol Cell Proteomics. 2023 Sep 18:100648. doi: 10.1016/j.mcpro.2023.100648. Online ahead of print.ABSTRACTThe evaluation of biopsied solid organ tissue has long relied on visual examination using a microscope. Immunohistochemistry is critical in this process, labeling and detecting cell lineage markers and therapeutic targets. However, while the practice of immunohistochemistry has reshaped diagnostic pathology and facilitated improvements in cancer treatment, it has also been subject to pervasive challenges with respect to standardization and reproducibility. Efforts are ongoing to improve immunohistochemistry, but for som...
Source: Molecular and Cellular Proteomics : MCP - September 20, 2023 Category: Molecular Biology Authors: William S Phipps Mark R Kilgore Jacob J Kennedy Jeffrey R Whiteaker Andrew N Hoofnagle Amanda G Paulovich Source Type: research

Protein kinase signaling networks driven by oncogenic Gq/11 in uveal melanoma identified by phosphoproteomic and bioinformatic analyses
Mol Cell Proteomics. 2023 Sep 18:100649. doi: 10.1016/j.mcpro.2023.100649. Online ahead of print.ABSTRACTMetastatic uveal melanoma (mUM) patients typically survive only 2-3 years because effective therapy does not yet exist. Here, to facilitate the discovery of therapeutic targets in UM, we have identified protein kinase signaling mechanisms elicited by the drivers in 90% of UM tumors: mutant constitutively active G protein α-subunits encoded by GNAQ (Gq) or GNA11 (G11). We used the highly specific Gq/11 inhibitor FR900359 (FR) to elucidate signaling networks that drive proliferation, metabolic reprogramming, and dediffer...
Source: Molecular and Cellular Proteomics : MCP - September 20, 2023 Category: Molecular Biology Authors: Michael D Onken Petra Erdmann-Gilmore Qiang Zhang Kisan Thapa Emily King Kevin M Kaltenbronn Sarah E Noda Carol M Makepeace Dennis Goldfarb Özgün Babur R Reid Townsend Kendall J Blumer Source Type: research

Targeted proteomic quantitation of NRF2 signaling and predictive biomarkers in HNSCC
Mol Cell Proteomics. 2023 Sep 14:100647. doi: 10.1016/j.mcpro.2023.100647. Online ahead of print.ABSTRACTThe NFE2L2(NRF2) oncogene and transcription factor drives a gene expression program that promotes cancer progression, metabolic reprogramming, immune evasion and chemoradiation resistance. Patient stratification by NRF2 activity may guide treatment decisions to improve outcome. Here, we developed a mass spectrometry (MS)-based targeted proteomics assay based on internal standard triggered parallel reaction monitoring (IS-PRM) to quantify 69 NRF2 pathway components and targets, as well as 21 proteins of broad clinical si...
Source: Molecular and Cellular Proteomics : MCP - September 16, 2023 Category: Molecular Biology Authors: Nathan T Wamsley Emily M Wilkerson Li Guan Kyle M LaPak Travis P Schrank Brittany J Holmes Robert W Sprung Petra Erdmann Gilmore Sophie P Gerndt Ryan S Jackson Randal C Paniello Patrik Pipkorn Sidharth V Puram Jason T Rich Reid R Townsend Jos é P Zevallo Source Type: research

Integrated Immunopeptidomics and Proteomics Study of SARS-CoV-2 Infected Calu-3 Cells Reveals Dynamic Changes in Allele-specific HLA Abundance and Antigen Presentation
We present an integrated immunopeptidomics and proteomics study of SARS-CoV-2 infection to comprehensively decipher the changes in host cells in response to viral infection. Immunopeptidomics analysis identified viral antigens presented by host cells through both Class I and Class II MHC system for recognition by the adaptive immune system. The host proteome changes were characterized by quantitative proteomics and glycoproteomics and from these data, the activation of TLR3-interferon pathway was identified. Glycosylation analysis of HLA proteins from the elution and flow-through of immunoprecipitation revealed that SARS-C...
Source: Molecular and Cellular Proteomics : MCP - September 14, 2023 Category: Molecular Biology Authors: Rui Chen Kelly M Fulton Anh Tran Diana Duque Kevin Kovalchik Etienne Caron Susan M Twine Jianjun Li Source Type: research