Molecular and Cellular Proteomics : MCP This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Secretome analysis: Reading cellular sign language to understand intercellular communication
Mol Cell Proteomics. 2023 Dec 9:100692. doi: 10.1016/j.mcpro.2023.100692. Online ahead of print.ABSTRACTA significant portion of mammalian proteomes is secreted to the extracellular space to fulfill crucial roles in cell-to-cell communication. To best recapitulate the intricate and multi-faceted crosstalk between cells in a live organism, there is an ever-increasing need for methods to study protein secretion in model systems that include multiple cell types. In addition, post-translational modifications further expand the complexity and versatility of cellular communication. This review aims to summarise recent strategies...
Source: Molecular and Cellular Proteomics : MCP - December 11, 2023 Category: Molecular Biology Authors: Wei Wu Jeroen Krijgsveld Source Type: research

Quantitative proteomics of tissue infiltrating T cells from CRC patients identified Lipocalin-2 induces T cell apoptosis and promotes tumor cell proliferation by iron efflux
In this study, we analyzed the protein expression pattern of the infiltrating T cells isolated from CRC patients using quantitative proteomics. CD4+ and CD8+ T cells were isolated from clinical samples and labeled by TMT reagents, and the DEPs were quantified by mass spectrometry. The T cell proteome profiling revealed dysfunctions in these tumor-infiltrating T cells. Specifically, anti-tumor immunity was suppressed due to differentially expressed metal ion transporters and immunity regulators. For the first time, Lipocalin-2 (LCN2) was shown to be significantly up-regulated in CD4+ T cells. Quantitative proteomic analysis...
Source: Molecular and Cellular Proteomics : MCP - December 10, 2023 Category: Molecular Biology Authors: Rui Che Qingsong Wang Minzhe Li Jian Shen Jianguo Ji Source Type: research

Into the dark serum proteome: personalized features of IgG1 and IgA1 repertoires in severe COVID-19 patients
Mol Cell Proteomics. 2023 Dec 6:100690. doi: 10.1016/j.mcpro.2023.100690. Online ahead of print.ABSTRACTSerum proteomics has matured and is now able to monitor hundreds of proteins quantitatively in large cohorts of patients. However, the fine characteristics of some of the most dominant proteins in serum, the immunoglobulins, are in these studies often ignored, due to their vast, and highly personalized, diversity in sequences. Here, we focus exclusively on these personalized features in the serum proteome, and distinctively chose to study individual samples from a low diversity population: elderly donors infected by SARS...
Source: Molecular and Cellular Proteomics : MCP - December 8, 2023 Category: Molecular Biology Authors: Albert Bondt Max Hoek Kelly Dingess Sem Tamara Bastiaan de Graaf Weiwei Peng Maurits A den Boer Mirjam Damen Ceri Zwart Arjan Barendregt Danique M H van Rijswijck Douwe Schulte Marloes Grobben Khadija Tejjani Jacqueline van Rijswijk Franziska V öllmy Joo Source Type: research

IMBAS-MS discovers organ-specific HLA peptide patterns in plasma
Mol Cell Proteomics. 2023 Dec 1:100689. doi: 10.1016/j.mcpro.2023.100689. Online ahead of print.ABSTRACTDistinction of non-self from self is the major task of the immune system. Immunopeptidomics studies the peptide repertoire presented by the human leukocyte antigen (HLA) protein, usually on tissues. However, HLA peptides are also bound to plasma soluble HLA (sHLA), but little is known about their origin and potential for biomarker discovery in this readily available biofluid. Currently, immunopeptidomics is hampered by complex workflows and limited sensitivity, typically requiring several mL of plasma. Here, we take adva...
Source: Molecular and Cellular Proteomics : MCP - December 3, 2023 Category: Molecular Biology Authors: Maria Wahle Marvin Thielert Maximilian Zwiebel Patricia Skowronek Wen-Feng Zeng Matthias Mann Source Type: research

Detection of pancreatic ductal adenocarcinoma-associated proteins in serum
Mol Cell Proteomics. 2023 Nov 27:100687. doi: 10.1016/j.mcpro.2023.100687. Online ahead of print.ABSTRACTPancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancer types, partly because it is frequently identified at an advanced stage, when surgery is no longer feasible. Therefore, early detection using minimally invasive methods such as blood tests may improve outcomes. However, studies to discover molecular signatures for the early detection of PDAC using blood tests have only been marginally successful. In the current study, a quantitative glycoproteomic approach via data-independent acquisition mass spect...
Source: Molecular and Cellular Proteomics : MCP - November 29, 2023 Category: Molecular Biology Authors: T Mamie Lih Liwei Cao Parham Minoo Gilbert S Omenn Ralph H Hruban Daniel W Chan Oliver F Bathe Hui Zhang Source Type: research

Identification of proteome-based immune subtypes of early hepatocellular carcinoma and analysis of potential metabolic drivers
In this study, we used a proteomic dataset to identify three immune subtypes (IM1-IM3) in HCC, each of which has distinctive clinical, immune, and metabolic characteristics. Among these subtypes, IM3 was found to have the poorest prognosis, with the highest levels of immune infiltration and T-cell exhaustion. Furthermore, IM3 showed elevated glycolysis and reduced bile acid metabolism, which was strongly correlated with CD8 T cell exhaustion and regulatory T-cell accumulation. Our study presents the proteomic immune stratification of HCC, revealing the possible link between immune cells and reprogramming of HCC glycolysis ...
Source: Molecular and Cellular Proteomics : MCP - November 26, 2023 Category: Molecular Biology Authors: Lihong Diao Mengqi He Binsheng Xu Lanhui Chen Ze Wang Yuting Yang Simin Xia Shengwei Hu Shuzhen Guo Dong Li Source Type: research