High-throughput screening and proteomic characterization of compounds targeting myeloid-derived suppressor cells
Mol Cell Proteomics. 2023 Aug 14:100632. doi: 10.1016/j.mcpro.2023.100632. Online ahead of print.ABSTRACTMyeloid-derived suppressor cells (MDSC) are a heterogeneous cell population of incompletely differentiated immune cells. They are known to suppress T cell activity and are implicated in multiple chronic diseases, which makes them an attractive cell population for drug discovery. Here, we characterized the baseline proteomes and phospho-proteomes of mouse MDSC differentiated from a progenitor cell line to a depth of 7,000 proteins and phosphorylation sites. We also validated the cellular system for drug discovery by reca...
Source: Molecular and Cellular Proteomics : MCP - August 16, 2023 Category: Molecular Biology Authors: Johannes Krumm Elissaveta Petrova Severin Lechner Julia Mergner Hans-Henning Boehm Alessandro Prestipino Dominik Steinbrunn Marshall L Deline Lisa Koetzner Christina Schindler Laura Helming Tobias Fromme Martin Klingenspor Hannes Hahne Jan-Carsten Pieck B Source Type: research

High-throughput screening and proteomic characterization of compounds targeting myeloid-derived suppressor cells
Mol Cell Proteomics. 2023 Aug 14:100632. doi: 10.1016/j.mcpro.2023.100632. Online ahead of print.ABSTRACTMyeloid-derived suppressor cells (MDSC) are a heterogeneous cell population of incompletely differentiated immune cells. They are known to suppress T cell activity and are implicated in multiple chronic diseases, which makes them an attractive cell population for drug discovery. Here, we characterized the baseline proteomes and phospho-proteomes of mouse MDSC differentiated from a progenitor cell line to a depth of 7,000 proteins and phosphorylation sites. We also validated the cellular system for drug discovery by reca...
Source: Molecular and Cellular Proteomics : MCP - August 16, 2023 Category: Molecular Biology Authors: Johannes Krumm Elissaveta Petrova Severin Lechner Julia Mergner Hans-Henning Boehm Alessandro Prestipino Dominik Steinbrunn Marshall L Deline Lisa Koetzner Christina Schindler Laura Helming Tobias Fromme Martin Klingenspor Hannes Hahne Jan-Carsten Pieck B Source Type: research

What can Ribo-seq, immunopeptidomics, and proteomics tell us about the non-canonical proteome?
Mol Cell Proteomics. 2023 Aug 10:100631. doi: 10.1016/j.mcpro.2023.100631. Online ahead of print.ABSTRACTRibosome profiling (Ribo-seq) has proven transformative for our understanding of the human genome and proteome by illuminating thousands of non-canonical sites of ribosome translation outside of the currently annotated coding sequences (CDSs). A conservative estimate suggests that at least 7,000 non-canonical open reading frames (ORFs) are translated, which, at first glance, has the potential to expand the number of human protein-coding sequences by 30%, from ∼19,500 annotated CDSs to over 26,000. Yet, additional scru...
Source: Molecular and Cellular Proteomics : MCP - August 12, 2023 Category: Molecular Biology Authors: John R Prensner Jennifer G Abelin Leron W Kok Karl R Clauser Jonathan M Mudge Jorge Ruiz-Orera Michal Bassani-Sternberg Robert L Moritz Eric W Deutsch Sebastiaan van Heesch Source Type: research

What can Ribo-seq, immunopeptidomics, and proteomics tell us about the non-canonical proteome?
Mol Cell Proteomics. 2023 Aug 10:100631. doi: 10.1016/j.mcpro.2023.100631. Online ahead of print.ABSTRACTRibosome profiling (Ribo-seq) has proven transformative for our understanding of the human genome and proteome by illuminating thousands of non-canonical sites of ribosome translation outside of the currently annotated coding sequences (CDSs). A conservative estimate suggests that at least 7,000 non-canonical open reading frames (ORFs) are translated, which, at first glance, has the potential to expand the number of human protein-coding sequences by 30%, from ∼19,500 annotated CDSs to over 26,000. Yet, additional scru...
Source: Molecular and Cellular Proteomics : MCP - August 12, 2023 Category: Molecular Biology Authors: John R Prensner Jennifer G Abelin Leron W Kok Karl R Clauser Jonathan M Mudge Jorge Ruiz-Orera Michal Bassani-Sternberg Robert L Moritz Eric W Deutsch Sebastiaan van Heesch Source Type: research

What can Ribo-seq, immunopeptidomics, and proteomics tell us about the non-canonical proteome?
Mol Cell Proteomics. 2023 Aug 10:100631. doi: 10.1016/j.mcpro.2023.100631. Online ahead of print.ABSTRACTRibosome profiling (Ribo-seq) has proven transformative for our understanding of the human genome and proteome by illuminating thousands of non-canonical sites of ribosome translation outside of the currently annotated coding sequences (CDSs). A conservative estimate suggests that at least 7,000 non-canonical open reading frames (ORFs) are translated, which, at first glance, has the potential to expand the number of human protein-coding sequences by 30%, from ∼19,500 annotated CDSs to over 26,000. Yet, additional scru...
Source: Molecular and Cellular Proteomics : MCP - August 12, 2023 Category: Molecular Biology Authors: John R Prensner Jennifer G Abelin Leron W Kok Karl R Clauser Jonathan M Mudge Jorge Ruiz-Orera Michal Bassani-Sternberg Robert L Moritz Eric W Deutsch Sebastiaan van Heesch Source Type: research