Molecular and Cellular Proteomics : MCP This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Integrative proteomic analysis reveals the cytoskeleton regulation and mitophagy difference between ischemic cardiomyopathy and dilated cardiomyopathy
This study facilitates a more profound comprehension of pathophysiologic heterogeneity between ICM and DCM, and provides a novel perspective to assist in the discovery of potential therapeutic targets for different types of heart failure.PMID:37852321 | DOI:10.1016/j.mcpro.2023.100667 (Source: Molecular and Cellular Proteomics : MCP)
Source: Molecular and Cellular Proteomics : MCP - October 18, 2023 Category: Molecular Biology Authors: Muyin Liu Linhui Zhai Zhaohua Yang Su Li Tianxian Liu Ao Chen Lulu Wang Youran Li Ruidong Li Chenguang Li Minjia Tan Zhangwei Chen Juying Qian Source Type: research

An automated nanowell-array workflow for quantitative multiplexed single-cell proteomics sample preparation at high sensitivity
Mol Cell Proteomics. 2023 Oct 13:100665. doi: 10.1016/j.mcpro.2023.100665. Online ahead of print.ABSTRACTMultiplexed and label-free mass spectrometry-based approaches with single-cell resolution have attributed surprising heterogeneity to presumed homogenous cell populations. Even though specialized experimental designs and instrumentation have demonstrated remarkable advances, the efficient sample preparation of single cells still lags. Here, we introduce the proteoCHIP, a universal option for single-cell proteomics sample preparation including multiplexed labeling up to 16-plex with high sensitivity and throughput. The a...
Source: Molecular and Cellular Proteomics : MCP - October 15, 2023 Category: Molecular Biology Authors: Claudia Ctortecka David Hartlmayr Anjali Seth Sasha Mendjan Guilhem Tourniaire Namrata D Udeshi Steven A Carr Karl Mechtler Source Type: research

High-resolution structural proteomics of mitochondria using the 'Build and Retrieve' methodology
Mol Cell Proteomics. 2023 Oct 13:100666. doi: 10.1016/j.mcpro.2023.100666. Online ahead of print.ABSTRACTThe application of integrated systems biology to the field of structural biology is a promising new direction, although it is still in the infant stages of development. Here we report the use of single particle cryo-electron microscopy (cryo-EM) to identify multiple proteins from three enriched heterogeneous fractions prepared from human liver mitochondrial lysate. We simultaneously identify and solve high-resolution structures of nine essential mitochondrial enzymes with key metabolic functions, including fatty acid ca...
Source: Molecular and Cellular Proteomics : MCP - October 15, 2023 Category: Molecular Biology Authors: Zhemin Zhang Marios L Tringides Christopher E Morgan Masaru Miyagi Jason A Mears Charles L Hoppel Edward W Yu Source Type: research

Global analysis of post-translational sidechain arginylation using pan-arginylation antibodies
Mol Cell Proteomics. 2023 Oct 11:100664. doi: 10.1016/j.mcpro.2023.100664. Online ahead of print.ABSTRACTArginylation is a post-translational modification mediated by the arginyltransferase ATE1, which transfers the amino acid arginine to a protein or peptide substrate from a tRNA molecule. Initially, arginylation was thought to occur only on N-terminally exposed acidic residues, and its function was thought to be limited to targeting proteins for degradation. However, more recent data has shown that ATE1 can arginylate sidechains of internal acidic residues in a protein without necessarily affecting metabolic stability. T...
Source: Molecular and Cellular Proteomics : MCP - October 13, 2023 Category: Molecular Biology Authors: Brittany MacTaggart Marie Shimogawa Marshall Lougee Hsin-Yao Tang E J Petersson Anna Kashina Source Type: research

Insight into the dynamics of the Ixodes ricinus nymphal midgut proteome
In this study, we used a label-free quantitative approach to perform a novel dynamic proteomic analysis of the midgut of Ixodes ricinus nymphs, covering their development from unfed to pre-moult stages. We identified 1,534 I. ricinus-specific proteins with a relatively low proportion of host proteins. This proteome dataset, which was carefully examined by manual scrutiny, allowed precise annotation of proteins important for blood meal processing and their dynamic changes during nymphal ontogeny. We focused on midgut molecules related to lipid hydrolysis, storage, and transport, opening an yet unexplored avenue for studying...
Source: Molecular and Cellular Proteomics : MCP - October 13, 2023 Category: Molecular Biology Authors: Tereza Kozelkov á Filip Dy čka Stephen Lu Veronika Urbanov á Helena Frantov á Daniel Sojka Radek Šíma Martin Horn Jan Perner Petr Kop áček Source Type: research

Proteomic and phosphoproteomic reprogramming in epithelial ovarian cancer metastasis
Mol Cell Proteomics. 2023 Oct 9:100660. doi: 10.1016/j.mcpro.2023.100660. Online ahead of print.ABSTRACTEpithelial ovarian cancer (EOC) is a high-risk cancer presenting with heterogeneous tumors. The high incidence of EOC metastasis from primary tumors to nearby tissues and organs is a major driver of EOC lethality. We used cellular models of spheroid formation and re-adherence to investigate cellular signaling dynamics in each step toward EOC metastasis. In our system, adherent cells model primary tumors, spheroids formation represents the initiation of metastatic spread, while re-adherent spheroid cells represent seconda...
Source: Molecular and Cellular Proteomics : MCP - October 11, 2023 Category: Molecular Biology Authors: Mallory I Frederick Owen F J Hovey Jenica H Kakadia Trevor G Shepherd Shawn S C Li Ilka U Heinemann Source Type: research

Chemical proteomic approach for in-depth glycosylation profiling of plasma carcinoembryonic antigen in cancer patients
Mol Cell Proteomics. 2023 Oct 9:100662. doi: 10.1016/j.mcpro.2023.100662. Online ahead of print.ABSTRACTCarcinoembryonic antigen (CEA) of human plasma is a biomarker of many cancer diseases, and its N-glycosylation accounts for 60% of molecular mass. It is highly desirable to characterize its glycoforms for providing additional dimension of features to increase its performance in prognosis and diagnosis of cancers. However, to systematically characterize its site-specific glycosylation is challenging due to its low abundance. Here, we developed a highly sensitive strategy for in-depth glycosylation profiling of plasma CEA ...
Source: Molecular and Cellular Proteomics : MCP - October 11, 2023 Category: Molecular Biology Authors: Jin Chen Lijun Yang Chang Li Luobin Zhang Weina Gao Ruilian Xu Ruijun Tian Source Type: research