Recent advances in the neural regulation of feeding behavior in adult Drosophila.
Abstract The ability to maintain metabolic homeostasis is a key capability critical for the survival and well-being of animals living in constantly changing environments. Metabolic homeostasis depends on neuromodulators, such as biogenic amines, neuropeptides, and hormones, to signal changes in animals' internal metabolic status and to orchestrate their behaviors accordingly. An important example is the regulation of feeding behavior by conserved molecular and cellular mechanisms across the animal kingdom. Its relatively simple brain coupled with well-characterized genetics and behavioral paradigms makes the fruit...
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Wang GH, Wang LM Tags: J Zhejiang Univ Sci B Source Type: research

Epigenetics recording varied environment and complex cell events represents the origin of cellular aging.
Abstract Although a relationship between epigenetics and aging phenotypic changes has been established, a theoretical explanation of the intrinsic connection between the epigenetics and aging is lacking. In this essay, we propose that epigenetic recording of varied cell environment and complex history could be an origin of cellular aging. Through epigenetic modifications, the environment and historical events can induce the chromatin template into an activated or repressive accessible structure, thereby shaping the DNA template into a spectrum of chromatin states. The inner nature of diversity and conflicts born b...
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Guo XJ, Yang D, Zhang XY Tags: J Zhejiang Univ Sci B Source Type: research

Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC.
Abstract To explore the volatile profiles and the contents of ten bioactive components (polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain, 17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and high-performance liquid chromatography (HPLC). A total of 75 volatile components were tentatively identified. Laomaner (LME), Laobanzhang (LBZ), and other teas on Bulang Mountain (BL) contained 70, 53, and 71 volatile compounds, respectively. Among the volatile compounds, alcohols (30....
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Zhang WJ, Liu C, Yang RJ, Zheng TT, Zhao MM, Ma L, Yan L Tags: J Zhejiang Univ Sci B Source Type: research

Improvement in post-partum uterine involution in rats treated with Apios americana.
CONCLUSIONS: These results, based on experimental observations at the molecular and protein levels, verified our hypothesis that Apios can improve uterine involution, and demonstrated the potential application of Apios in post-partum care. PMID: 31168971 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Zheng ZH, Han Y, You SY, Chen Z, Zheng XD Tags: J Zhejiang Univ Sci B Source Type: research

Systematic review and meta-analysis of the efficacy and safety of immunosuppressive pulse therapy in the treatment of paraquat poisoning.
Abstract Paraquat (PQ), a highly effective herbicide, is widely used worldwide. PQ poisoning can cause multiple organ failure, in which the lung is the primary target organ. After PQ poisoning, the patient mortality rate is as high as 90%, and there is currently no specific antidote. The main clinical treatment is the use of glucocorticoids and cyclophosphamide for pulse therapy, but its effectiveness and safety are still uncertain. We investigated the effectiveness and safety of immunosuppressive pulse therapy with glucocorticoids and cyclophosphamide to evaluate the treatment value in patients with acute PQ pois...
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Xu YG, Lu YQ Tags: J Zhejiang Univ Sci B Source Type: research

Relationship between genetic variation in the α2A-adrenergic receptor and the cardiovascular effects of dexmedetomidine in the Chinese Han population.
Relationship between genetic variation in the α2A-adrenergic receptor and the cardiovascular effects of dexmedetomidine in the Chinese Han population. J Zhejiang Univ Sci B. 2019 Jul;20(7):598-604 Authors: Zhu SJ, Wang KR, Zhang XX, Zhu SM Abstract There are differences in individual cardiovascular responses to the administration of dexmedetomidine, a highly selective α2A-adrenergic receptor (ADRA2A) agonist. The aim of this study was to investigate ADRA2A gene polymorphisms in the Chinese Han population and their association with the cardiovascular response to intravenous dexmedetomidine ...
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Zhu SJ, Wang KR, Zhang XX, Zhu SM Tags: J Zhejiang Univ Sci B Source Type: research

Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation.
Abstract Acute cellular rejection (ACR) remains a major concern after liver transplantation. Predicting and monitoring acute rejection by non-invasive methods are very important for guiding the use of immunosuppressive drugs. Many studies have shown that exosomes and their contents are potential biomarkers for various liver diseases. Here, we identify and validate the role of exosomes and galectin-9 in ACR after liver transplantation. Exosomes were isolated from three sets of paired patients, with and without ACR, and the proteins within the exosomes were isolated and identified. Candidate proteins were then valid...
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Zhang AB, Peng YF, Jia JJ, Nie Y, Zhang SY, Xie HY, Zhou L, Zheng SS Tags: J Zhejiang Univ Sci B Source Type: research

Hypolipidemic activity of low-cholesterol ovum oil of Rana chensinensis and phytosterol (stigmasterol) in rats.
Abstract The ovum oil of forest frog has various health beneficial functions. In the current research, we evaluated the hypolipidemic effects of the low-cholesterol ovum oil from the forest frog and its combination with stigmasterol in rats. PMID: 31168975 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - June 8, 2019 Category: Science Authors: Zhao CH, Zhao C, Ye HQ, Xi CY, Zou ZP, Zhou XH, Hou PP, Zhang TH Tags: J Zhejiang Univ Sci B Source Type: research

p53 and its isoforms in DNA double-stranded break repair.
Abstract DNA double-stranded break (DSB) is one of the most catastrophic damages of genotoxic insult. Inappropriate repair of DNA DSBs results in the loss of genetic information, mutation, and the generation of harmful genomic rearrangements, which predisposes an organism to immunodeficiency, neurological damage, and cancer. The tumor repressor p53 plays a key role in DNA damage response, and has been found to be mutated in 50% of human cancer. p53, p63, and p73 are three members of the p53 gene family. Recent discoveries have shown that human p53 gene encodes at least 12 isoforms. Different p53 members and isofor...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Zhang YX, Pan WY, Chen J Tags: J Zhejiang Univ Sci B Source Type: research

Novel insights into cell cycle regulation of cell fate determination.
Abstract The stem/progenitor cell has long been regarded as a central cell type in development, homeostasis, and regeneration, largely owing to its robust self-renewal and multilineage differentiation abilities. The balance between self-renewal and stem/progenitor cell differentiation requires the coordinated regulation of cell cycle progression and cell fate determination. Extensive studies have demonstrated that cell cycle states determine cell fates, because cells in different cell cycle states are characterized by distinct molecular features and functional outputs. Recent advances in high-resolution epigenome ...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Gao SW, Liu F Tags: J Zhejiang Univ Sci B Source Type: research

Genomic data mining for functional annotation of human long noncoding RNAs.
Abstract Life may have begun in an RNA world, which is supported by increasing evidence of the vital role that RNAs perform in biological systems. In the human genome, most genes actually do not encode proteins; they are noncoding RNA genes. The largest class of noncoding genes is known as long noncoding RNAs (lncRNAs), which are transcripts greater in length than 200 nucleotides, but with no protein-coding capacity. While some lncRNAs have been demonstrated to be key regulators of gene expression and 3D genome organization, most lncRNAs are still uncharacterized. We thus propose several data mining and machine le...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Gudenas BL, Wang J, Kuang SZ, Wei AQ, Cogill SB, Wang LJ Tags: J Zhejiang Univ Sci B Source Type: research

Exosomal long non-coding RNAs: biological properties and therapeutic potential in cancer treatment.
Abstract Exosomes and long non-coding RNAs (lncRNAs) are emerging as important elements contributing to a more comprehensive understanding of cancer development and progression. The discovery of lncRNAs in exosomes further indicates their bona fide biological functional roles in cancer development and drug resistance. In this review, we describe the biogenesis of exosomes and summarize the function of exosomal lncRNAs in the field of cancer research. These findings strikingly advance current knowledge of exosomal lncRNAs and suggest that they may be promising diagnostic biomarkers and therapeutic targets for cance...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Luo J, Xiong Y, Fu PF, Li EC, Qu L, Fan X, Cai ZJ, Lin AF Tags: J Zhejiang Univ Sci B Source Type: research

On the necessity of an integrative approach to understand protein structural dynamics.
Abstract Proteins are dynamic, fluctuating between multiple conformational states. Protein dynamics, spanning orders of magnitude in time and space, allow proteins to perform specific functions. Moreover, under certain conditions, proteins can morph into a different set of conformations. Thus, a complete understanding of protein structural dynamics can provide mechanistic insights into protein function. Here, we review the latest developments in methods used to determine protein ensemble structures and to characterize protein dynamics. Techniques including X-ray crystallography, cryogenic electron microscopy, and ...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Yang QF, Tang C Tags: J Zhejiang Univ Sci B Source Type: research

Pericarp and seed of litchi and longan fruits: constituent, extraction, bioactive activity, and potential utilization.
Abstract Litchi (Litchi chinensis Sonn.) and longan (Dimocarpus longan Lour.) fruits have a succulent and white aril with a brown seed and are becoming popular worldwide. The two fruits have been used in traditional Chinese medicine as popular herbs in the treatment of neural pain, swelling, and cardiovascular disease. The pericarp and seed portions as the by-products of litchi and longan fruits are estimated to be approximately 30% of the dry weight of the whole fruit and are rich in bioactive constituents. In the recent years, many biological activities, such as tyrosinase inhibitory, antioxidant, anti-inflammat...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Zhu XR, Wang H, Sun J, Yang B, Duan XW, Jiang YM Tags: J Zhejiang Univ Sci B Source Type: research

Mechanisms and regulation of aluminum-induced secretion of organic acid anions from plant roots.
Abstract Aluminum (Al) is the most abundant metal element in the earth's crust. On acid soils, at pH 5.5 or lower, part of insoluble Al-containing minerals become solubilized into soil solution, with resultant highly toxic effects on plant growth and development. Nevertheless, some plants have developed Al-tolerance mechanisms that enable them to counteract this Al toxicity. One such well-documented mechanism is the Al-induced secretion of organic acid anions, including citrate, malate, and oxalate, from plant roots. Once secreted, these anions chelate external Al ions, thus protecting the secreting plant from Al ...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Yang JL, Fan W, Zheng SJ Tags: J Zhejiang Univ Sci B Source Type: research

Bacterial degradation of anthraquinone dyes.
Abstract Anthraquinone dyes, which contain anthraquinone chromophore groups, are the second largest class of dyes after azo dyes and are used extensively in textile industries. The majority of these dyes are resistant to degradation because of their complex and stable structures; consequently, a large number of anthraquinone dyes find their way into the environment causing serious pollution. At present, the microbiological approach to treating printing and dyeing wastewater is considered to be an economical and feasible method, and reports regarding the bacterial degradation of anthraquinone dyes are increasing. T...
Source: J Zhejiang Univ Sci ... - May 17, 2019 Category: Science Authors: Li HH, Wang YT, Wang Y, Wang HX, Sun KK, Lu ZM Tags: J Zhejiang Univ Sci B Source Type: research

A special issue to mark the 90th Anniversary of College of Life Sciences, Zhejiang University.
Abstract The College of Life Sciences (CLS) remains one of the most prestigious-and the oldest-colleges in Zhejiang University. This special issue, which includes 16 reviews contributed by our alumni and faculties, is dedicated to mark the 90th Anniversary of CLS. The reviews provide a glimpse of current progresses in the areas of life sciences such as biochemical processes and their association with diseases (Ding et al., 2019; Hu et al., 2019; Jin et al., 2019; Nie and Yi, 2019), cancer biology (Feng, 2019; Huang et al., 2019; Leonard and Zhang, 2019; Zhu F et al., 2019), plant and environmental microbiology (Li...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Peng JR, Guan KL, Hong DY, Lin XH, Yang HM, Zhu YX Tags: J Zhejiang Univ Sci B Source Type: research

Tumor immunology and immunotherapy: a journey I started from Hangzhou.
Abstract This short article is dedicated to the 90th Anniversary of the School of Life Sciences at Zhejiang University, China. Immunotherapy of cancer is currently a hot topic in the biomedical field, and a re-search focus of my laboratory is on developing new and effective combinatorial immunotherapeutic strategies for liver cancer. Of note, my interest in immunotherapy of cancer stems from the training as an undergraduate student at Hangzhou University, China, almost 40 years ago. PMID: 31090263 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Feng GS Tags: J Zhejiang Univ Sci B Source Type: research

Estrogen receptor coactivator Mediator Subunit 1 (MED1) as a tissue-specific therapeutic target in breast cancer.
Abstract Breast cancer, one of the most frequent cancer types, is a leading cause of death in women worldwide. Estrogen receptor (ER) α is a nuclear hormone receptor that plays key roles in mammary gland development and breast cancer. About 75% of breast cancer cases are diagnosed as ER-positive; however, nearly half of these cancers are either intrinsically or inherently resistant to the current anti-estrogen therapies. Recent studies have identified an ER coactivator, Mediator Subunit 1 (MED1), as a unique, tissue-specific cofactor that mediates breast cancer metastasis and treatment resistance. MED1 is ov...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Leonard M, Zhang X Tags: J Zhejiang Univ Sci B Source Type: research

Role of SIRT1 in hematologic malignancies.
Abstract Sirtuin 1 (SIRT1) is a protein deacetylase, which regulates various physiological activities by deacetylating different protein substrates. An increasing number of studies have revealed critical roles of SIRT1 in different aspects of cancers including metabolism, proliferation, genomic instability, and chemotherapy resistance. Depending on the protein targets in a certain oncogenic context, SIRT1 may play a unique role in each individual blood cancer subtype. Our previous work showed that activation of SIRT1 in primitive leukemia cells of acute myeloid leukemia (AML) and chronic myelogenous leukemia (CML)...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Huang FT, Sun J, Zhang L, He X, Zhu YH, Dong HJ, Wang HY, Zhu L, Zou JY, Huang JW, Li L Tags: J Zhejiang Univ Sci B Source Type: research

Complex roles of necroptosis in cancer.
Abstract Necroptosis is a tightly regulated form of necrosis that requires the activation of receptor-interacting protein (RIP) kinases RIPK1 and RIPK3, as well as the RIPK3 substrate mixed lineage kinase domain-like protein (MLKL). Because of membrane rupture, necroptotic cells release damage-associated molecular patterns (DAMPs) that evoke immune responses. Necroptosis is emerging as an important cellular response in the modulation of cancer initiation, progression, and metastasis. Necroptosis of cancer cells is considered to be an immunogenic cell death capable of activating anti-tumor immunity. Necroptosis als...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Zhu F, Zhang W, Yang T, He SD Tags: J Zhejiang Univ Sci B Source Type: research

Bile-ology: from bench to bedside.
Abstract Bile acids (BAs) are originally known as detergents essential for the digestion and absorption of lipids. In recent years, extensive research has unveiled new functions of BAs as gut hormones that modulate physiological and pathological processes, including glucose and lipid metabolism, energy expenditure, inflammation, tumorigenesis, cardiovascular disease, and even the central nervous system in addition to cholesterol homeostasis, enterohepatic protection and liver regeneration. BAs are closely linked with gut microbiota which might explain some of their crucial roles in organs. The signaling actions of...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Jin LH, Fang ZP, Fan MJ, Huang WD Tags: J Zhejiang Univ Sci B Source Type: research

Roles of hyaluronan in cardiovascular and nervous system disorders.
Abstract Hyaluronan is a widely occurring extracellular matrix molecule, which is not only a supporting structural component, but also an active regulator of cellular functions. The chemophysical and biological properties of hyaluronan are greatly affected by its molecular size and several hyaluronan-binding proteins, making hyaluronan a fascinating molecule with great functional diversity. This review summarizes our current understanding of the roles of hyaluronan in cardiovascular and nervous system disorders, such as atherosclerosis, myocardial infarction, and stroke, with the aim to provide a foundation for fu...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Ding HY, Xie YN, Dong Q, Kimata K, Nishida Y, Ishiguro N, Zhuo LS Tags: J Zhejiang Univ Sci B Source Type: research

O-GlcNAcylation, a sweet link to the pathology of diseases.
Abstract O-linked N-acetylglucosamine (O-GlcNAc) is a dynamic post-translational modification occurring on myriad proteins in the cell nucleus, cytoplasm, and mitochondria. The donor sugar for O-GlcNAcylation, uridine-diphosphate N-acetylglucosamine (UDP-GlcNAc), is synthesized from glucose through the hexosamine biosynthetic pathway (HBP). The recycling of O-GlcNAc on proteins is mediated by two enzymes in cells-O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which catalyze the addition and removal of O-GlcNAc, respectively. O-GlcNAcylation is involved in a number of important cell processes including transcrip...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Nie H, Yi W Tags: J Zhejiang Univ Sci B Source Type: research

Cell models and drug discovery for mitochondrial diseases.
Abstract Mitochondrion is a semi-autonomous organelle, important for cell energy metabolism, apoptosis, the production of reactive oxygen species (ROS), and Ca2+ homeostasis. Mitochondrial DNA (mtDNA) mutation is one of the primary factors in mitochondrial disorders. Though much progress has been made, there remain many difficulties in constructing cell models for mitochondrial diseases. This seriously restricts studies related to targeted drug discovery and the mechanism and therapy for such diseases. Here we summarize the characteristics of patient-specific immortalized lymphoblastoid cells, fibroblastoid cells,...
Source: J Zhejiang Univ Sci ... - May 1, 2019 Category: Science Authors: Hu SY, Zhuang QQ, Qiu Y, Zhu XF, Yan QF Tags: J Zhejiang Univ Sci B Source Type: research

Advances in low-frequency ultrasound combined with microbubbles in targeted tumor therapy.
Abstract The development of low-frequency ultrasound imaging technology and the improvement of ultrasound contrast agent production technology mean that they play an increasingly important role in tumor therapy. The interaction between ultrasound and microbubbles and their biological effects can transfer and release microbubbles carrying genes and drugs to target tissues, mediate the apoptosis of tumor cells, and block the embolization of tumor microvasculature. With the optimization of ultrasound parameters, the development of targeted microbubbles, and the emergence of various composite probes with both diagnost...
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Wang LY, Zheng SS Tags: J Zhejiang Univ Sci B Source Type: research

Simulation of inter atrial block based on a human atrial model.
Abstract Inter atrial block (IAB) is a prevailing cardiac conduction abnormality that is under-recognized in clinical practice. IAB has strong association with atrial arrhythmia, left atrial enlargement, and electromechanical discordance, increasing the risk of atrial fibrillation (AF) and myocardial ischemia. IAB was generally believed to be caused by impaired conduction along the Bachmann bundle (BB). However, there are three other conduction pathways, including the fibers posteriorly in the vicinity of the right pulmonary veins (VRPV), transseptal fibers in the fossa ovalis (FO), and muscular bundles on the inf...
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Gao Y, Gong YL, Xia L, Zheng DC Tags: J Zhejiang Univ Sci B Source Type: research

Mitochondrial superoxide anions induced by exogenous oxidative stress determine tumor cell fate: an individual cell-based study.
CONCLUSIONS: Results from a modified microchip demonstrated that 1 mmol/L H2O2 induced a rapid increase in cellular O2 •- levels (>27 vs.>406 amol in 20 min), leading to increased cellular oxidizing power (evaluated by ROS levels) and decreased reducing power (evaluated by glutathione (GSH) levels). In addition, we examined the dynamics of cytosolic Ca2+ and mitochondrial Ca2+ by confocal laser scanning microscopy and confirmed that Ca2+ stores in the endoplasmic reticulum were the primary source of H2O2-induced cytosolic Ca2+ bursts. It is clear that mitochondria have pivotal roles in determining how exogenous ...
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Pan H, Wang BH, Li ZB, Gong XG, Qin Y, Jiang Y, Han WL Tags: J Zhejiang Univ Sci B Source Type: research

Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia (Sinningia speciosa).
Abstract We investigated the microRNA172 (miR172)-mediated regulatory network for the perception of changes in external and endogenous signals to identify a universally applicable floral regulation system in ornamental plants, manipulation of which could be economically beneficial. Transgenic gloxinia plants, in which miR172 was either overexpressed or suppressed, were generated using Agrobacterium-mediated transformation. They were used to study the effect of altering the expression of this miRNA on time of flowering and to identify its mRNA target. Early or late flowering was observed in transgenic plants in whi...
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Li XY, Guo F, Ma SY, Zhu MY, Pan WH, Bian HW Tags: J Zhejiang Univ Sci B Source Type: research

Screening, purification, and characterization of an extracellular lipase from Aureobasidium pullulans isolated from stuffed buns steamers.
Abstract An extracellular lipase from Aureobasidium pullulans was obtained and purified with a specific activity of 17.7 U/mg of protein using ultrafiltration and a DEAE-Sepharose Fast Flow column. Characterization of the lipase indicated that it is a novel finding from the species A. pullulans. The molecular weight of the lipase was 39.5 kDa, determined by sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited its optimum activity at 40 °C and pH of 7. It also showed a remarkable stability in some organic solutions (30%, v/v) including n-propanol, isopropanol, dimethyl su...
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Li Y, Liu TJ, Zhao MJ, Zhang H, Feng FQ Tags: J Zhejiang Univ Sci B Source Type: research

Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus.
In this study, two monoclonal antibodies (MAbs) 16E6 and 11C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper (SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and ...
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Huang DQ, Chen R, Wang YQ, Hong J, Zhou XP, Wu JX Tags: J Zhejiang Univ Sci B Source Type: research

Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis.
CONCLUSIONS: Dexamethasone protects the endothelial glycocalyx from inflammatory degradation possibly initiated by TNF-α during SAP. This is might be a significant discovery that helps to prevent tissue edema and hypoperfusion in the future. PMID: 30932380 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Yu WQ, Zhang SY, Fu SQ, Fu QH, Lu WN, Zhang J, Liang ZY, Zhang Y, Liang TB Tags: J Zhejiang Univ Sci B Source Type: research

Incidence and clinical correlates of anger attacks in Chinese patients with obsessive-compulsive disorder.
CONCLUSIONS: These data suggest that anger attacks are relatively common in Chinese patients with OCD. The severity of anger attacks is related to educational level, obsessing symptoms, and anxiety, which may be a latent variable reflecting executive functioning and emotion regulation skills. PMID: 30932381 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - April 1, 2019 Category: Science Authors: Zhang YY, Gong HF, Zhang XL, Liu WJ, Jin HY, Fang F, Schneider S, Mcingvale E, Zhang CC, Goodman WK, Sun XR, Storch EA Tags: J Zhejiang Univ Sci B Source Type: research

Comprehensive therapeutics targeting the corticospinal tract following spinal cord injury.
Abstract Spinal cord injury (SCI), which is much in the public eye, is still a refractory disease compromising the well-being of both patients and society. In spite of there being many methods dealing with the lesion, there is still a deficiency in comprehensive strategies covering all facets of this damage. Further, we should also mention the structure called the corticospinal tract (CST) which plays a crucial role in the motor responses of organisms, and it will be the focal point of our attention. In this review, we discuss a variety of strategies targeting different dimensions following SCI and some treatments...
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Xu AK, Gong Z, He YZ, Xia KS, Tao HM Tags: J Zhejiang Univ Sci B Source Type: research

MiR-1180 from bone marrow-derived mesenchymal stem cells induces glycolysis and chemoresistance in ovarian cancer cells by upregulating the Wnt signaling pathway.
CONCLUSIONS: BM-MSCs enhance the chemoresistance of ovarian cancer by releasing miR-1180. The released miR-1180 activates the Wnt signaling pathway in cancer cells by targeting SFRP1. The enhanced Wnt signaling upregulates the glycolytic level (i.e. Warburg effect), which reinforces the chemoresistance property of ovarian cancer cells. PMID: 30829010 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Gu ZW, He YF, Wang WJ, Tian Q, Di W Tags: J Zhejiang Univ Sci B Source Type: research

Ambient air pollution and adverse birth outcomes: a systematic review and meta-analysis.
The objective of this study was to assess this correlation quantitatively and to explore sources of heterogeneity. We included all published case-control or cohort studies that evaluated the correlation between ambient air pollution and low birth weight (LBW), preterm birth (PTB), and small for gestational age (SGA). Analytical methods and inclusion criteria were provided on the PROSPERO website (CRD42018085816). We evaluated pooled effects and heterogeneity. Subgroup analyses (grouped by exposure period, study settings, study design, exposure types, data source, Newcastle-Ottawa quality score (NOS), and adjustment for smo...
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Guo LQ, Chen Y, Mi BB, Dang SN, Zhao DD, Liu R, Wang HL, Yan H Tags: J Zhejiang Univ Sci B Source Type: research

Valproic acid withdrawal ameliorates impairments of hippocampal-spatial working memory and neurogenesis.
Abstract Valproic acid (VPA), an agent that is used to treat epileptic seizures, can cause spatial memory impairment in adults and children. This effect is thought to be due to the ability of VPA to inhibit neurogenesis in the hippocampus, which is required for learning. We have previously used an animal model to show that VPA significantly impairs hippocampal-spatial working memory and inhibits neuronal generation in the sub-granular zone of the dentate gyrus. As there are patient reports of improvements in memory after discontinuing VPA treatment, the present study investigated the recovery of both spatial memor...
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Pannangrong W, Sirichoat A, Wongsiri T, Wigmore P, Welbat JU Tags: J Zhejiang Univ Sci B Source Type: research

A simplified and miniaturized glucometer-based assay for the detection of β-glucosidase activity.
A simplified and miniaturized glucometer-based assay for the detection of β-glucosidase activity. J Zhejiang Univ Sci B. 2019 Mar.;20(3):264-272 Authors: Jin MY, Zhang T, Yang YS, Ding Y, Li JS, Zhong GR Abstract β-Glucosidase activity assays constitute an important indicator for the early diagnosis of neonatal necrotizing enterocolitis and qualitative changes in medicinal plants. The drawbacks of the existing methods are high consumption of both time and reagents, complexity in operation, and requirement of expensive instruments and highly trained personnel. The present study provides a sim...
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Jin MY, Zhang T, Yang YS, Ding Y, Li JS, Zhong GR Tags: J Zhejiang Univ Sci B Source Type: research

Enhanced water solubility, antioxidant activity, and oral absorption of hesperetin by D- α-tocopheryl polyethylene glycol 1000 succinate and phosphatidylcholine.
Enhanced water solubility, antioxidant activity, and oral absorption of hesperetin by D-α-tocopheryl polyethylene glycol 1000 succinate and phosphatidylcholine. J Zhejiang Univ Sci B. 2019 Mar.;20(3):273-281 Authors: Gu SF, Wang LY, Tian YJ, Zhou ZX, Tang JB, Liu XR, Jiang HP, Shen YQ Abstract Hesperetin, an abundant bioactive component of citrus fruits, is poorly water-soluble, resulting in low oral bioavailability. We developed new formulations to improve the water solubility, antioxidant activity, and oral absorption of hesperetin. Two nano-based formulations were developed, namely hesperetin...
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Gu SF, Wang LY, Tian YJ, Zhou ZX, Tang JB, Liu XR, Jiang HP, Shen YQ Tags: J Zhejiang Univ Sci B Source Type: research

Clinical application of cross microsurgical vasovasostomy in scrotum for atypical obstructive azoospermia.
Abstract Seminal duct obstruction may result in obstructive azoospermia (OA) and severe oligoasthenoteratozoospermia (OAT) (
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Liang ZY, Zhang FB, Li LJ, Li JP, Wu JG, Chen C, Zhu YM Tags: J Zhejiang Univ Sci B Source Type: research

Genetic distribution and forensic evaluation of multiplex autosomal short tandem repeats in the Chinese Xinjiang Mongolian group.
Abstract To further enrich the genetic data of the Chinese Xinjiang Mongolian group, the genetic distribution and forensic parameters of 19 autosomal short tandem repeats (STRs) were investigated. Altogether, 249 alleles were observed in these 19 STRs. The mean values of the polymorphism information content (PIC), match probability (MP), discrimination power (DP), and probability of exclusion (PE) for these 19 STRs were 0.7775, 0.0699, 0.9301, and 0.6085, respectively. Additionally, the cumulative DP and PE values obtained in the Mongolian group were 0.999 999 999 999 999 999 999 995 67 and 0.999 999 992 163, resp...
Source: J Zhejiang Univ Sci ... - March 1, 2019 Category: Science Authors: Wei YY, Jin XY, Lan Q, Cui W, Chen C, Kong TT, Guo YX, Chen JG, Zhu BF Tags: J Zhejiang Univ Sci B Source Type: research

Frontiers in auditory bioscience and technology: a special feature on recent advances in hearing research.
Abstract Hearing impairment has become one of the most common sensory disabilities. The World Health Organization (WHO) estimates that 466 million people were living with disabling hearing loss in 2018, and that number could rise to 900 million by 2050. Conductive hearing loss, which predominantly involves the sound-transmitting route of the outer and middle ear, has been well handled by antibiotics and surgery. However, sensorineural hearing loss, which involves the inner ear and structures further within the auditory pathway, has very limited biological treatment options (current treatment options include only h...
Source: J Zhejiang Univ Sci ... - January 24, 2019 Category: Science Authors: Cheng YF Tags: J Zhejiang Univ Sci B Source Type: research

Cerebrospinal fluid otorrhea secondary to congenital inner ear dysplasia: diagnosis and management of 18 cases.
CONCLUSIONS: CSF otorrhea due to congenital inner ear dysplasia is more severe in children than in adults. The most common symptoms were meningitis, hearing impairment, and CSF otorrhea or rhinorrhea. HRCT has high diagnostic accuracy for this disease. The most common fistula site was around the oval window, including the stapes footplates and the annular ligament. PMID: 30666849 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - January 24, 2019 Category: Science Authors: Wang B, Dai WJ, Cheng XT, Liuyang WY, Yuan YS, Dai CF, Shu YL, Chen B Tags: J Zhejiang Univ Sci B Source Type: research

Oral administration of Lactobacillus rhamnosus GG to newborn piglets augments gut barrier function in pre-weaning piglets.
Abstract To understand the effects of Lactobacillus rhamnosus GG (ATCC 53103) on intestinal barrier function in pre-weaning piglets under normal conditions, twenty-four newborn littermate piglets were randomly divided into two groups. Piglets in the control group were orally administered with 2 mL 0.1 g/mL sterilized skim milk while the treatment group was administered the same volume of sterilized skim milk with the addition of viable L. rhamnosus at the 1st, 3rd, and 5th days after birth. The feeding trial was conducted for 25 d. Results showed that piglets in the L. rhamnosus group exhibited increased weaning w...
Source: J Zhejiang Univ Sci ... - January 24, 2019 Category: Science Authors: Wang Y, Gong L, Wu YP, Cui ZW, Wang YQ, Huang Y, Zhang XP, Li WF Tags: J Zhejiang Univ Sci B Source Type: research

Novel ractopamine-protein carrier conjugation and its application to the lateral flow strip test for ractopamine detection in animal feed.
Abstract In this work, a novel conjugate of ractopamine and bovine serum albumin (RAC-BSA) has been developed via the Mannich reaction, with a mole coupling ratio for RAC-BSA of 9:1. The proposed conjugation method provides a simple and one-step method with the use of fewer reagents compared with other conjugation methods for competitive immunoassays. RAC-BSA conjugation was used to fabricate a competitive lateral flow strip test for RAC detection in animal feed. For sample preparation, RAC was spiked in swine feed purchased from the local markets in Thailand, and methanol and running buffer at a volume ratio of 1...
Source: J Zhejiang Univ Sci ... - January 24, 2019 Category: Science Authors: Preechakasedkit P, Ngamrojanavanich N, Khongchareonporn N, Chailapakul O Tags: J Zhejiang Univ Sci B Source Type: research

Role of cytoplasmic lncRNAs in regulating cancer signaling pathways.
Abstract Cancer remains a serious healthcare problem despite significant improvements in early detection and treatment approaches in the past few decades. Novel biomarkers for diagnosis and therapeutic strategies are urgently needed. In recent years, long noncoding RNAs (lncRNAs) have been reported to be aberrantly expressed in tumors and show crosstalk with key cancer-related signaling pathways. In this review, we summarized the current progress of research on cytoplasmic lncRNAs and their roles in regulating cancer signaling and tumor progression, further characterization of which may lead to effective approache...
Source: J Zhejiang Univ Sci ... - January 1, 2019 Category: Science Authors: Fu PF, Zheng X, Fan X, Lin AF Tags: J Zhejiang Univ Sci B Source Type: research

A systematic review of herbal medicines for the treatment of cancer cachexia in animal models.
CONCLUSIONS: This study showed that herbal medicines might be a useful approach for treating cancer cachexia. However, more detailed experimental studies on the molecular mechanisms and active compounds are needed. PMID: 30614226 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - January 1, 2019 Category: Science Authors: Park B, You S, Cho WCS, Choi JY, Lee MS Tags: J Zhejiang Univ Sci B Source Type: research

Ethical considerations of cellular immunotherapy for cancer.
Abstract With the rapid development of immunology, molecular biology, and associated technologies such as next-generation sequencing, cellular immunotherapy has recently become the fourth major cancer treatment. Immunotherapies based on T cells, natural killer cells, and dendritic cells play key roles in cancer immunotherapy. However, their application in clinical practice raises several ethical issues. Thus, studies should focus on proper adherence to basic ethical principles that can effectively guide and solve related clinical problems in the course of treatment, improve treatment effects, and protect the right...
Source: J Zhejiang Univ Sci ... - January 1, 2019 Category: Science Authors: Ren SS, Deng JW, Hong M, Ren YL, Fu HJ, Liu YN, Chen Z Tags: J Zhejiang Univ Sci B Source Type: research

Experiments that led to the first gene-edited babies: the ethical failings and the urgent need for better governance.
Abstract The rapid developments of science and technology in China over recent decades, particularly in biomedical research, have brought forward serious challenges regarding ethical governance. Recently, Jian-kui HE, a Chinese scientist, claimed to have "created" the first gene-edited babies, designed to be naturally immune to the human immunodeficiency virus (HIV). The news immediately triggered widespread criticism, denouncement, and debate over the scientific and ethical legitimacy of HE's genetic experiments. China's guidelines and regulations have banned germline genome editing on human embryos for...
Source: J Zhejiang Univ Sci ... - January 1, 2019 Category: Science Authors: Li JR, Walker S, Nie JB, Zhang XQ Tags: J Zhejiang Univ Sci B Source Type: research

Interferon- γ regulates cell malignant growth via the c-Abl/HDAC2 signaling pathway in mammary epithelial cells.
In this study, we investigated the mechanisms underlying the malignant proliferation of BMECs under IFN-γ treatment. The primary BMECs used in this study were stimulated by IFN-γ (10 ng/mL) for a long term to promote malignancy. We observed that IFN-γ could promote malignant cell proliferation, increase the expression of cyclin D1/cyclin-dependent kinase 4 (CDK4), decrease the expression of p21, and upregulate the expression of cellular-abelsongene (c-Abl) and histone deacetylase 2 (HDAC2). The HDAC2 inhibitor, valproate (VPA) and the c-Abl inhibitor, imatinib, lowered the expression level of cyclin D1/CD...
Source: J Zhejiang Univ Sci ... - January 1, 2019 Category: Science Authors: Ren WB, Xia XJ, Huang J, Guo WF, Che YY, Huang TH, Lei LC Tags: J Zhejiang Univ Sci B Source Type: research