Mass spectrometry-based protein ‒protein interaction techniques and their applications in studies of DNA damage repair.
Mass spectrometry-based protein‒protein interaction techniques and their applications in studies of DNA damage repair. J Zhejiang Univ Sci B. 2021 Jan 15;22(1):1-20 Authors: Chen Z, Chen J Abstract Proteins are major functional units that are tightly connected to form complex and dynamic networks. These networks enable cells and organisms to operate properly and respond efficiently to environmental cues. Over the past decades, many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected. At the same time...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Chen Z, Chen J Tags: J Zhejiang Univ Sci B Source Type: research

ADP-ribosylhydrolases: from DNA damage repair to COVID-19.
Abstract Adenosine diphosphate (ADP)-ribosylation is a unique post-translational modification that regulates many biological processes, such as DNA damage repair. During DNA repair, ADP-ribosylation needs to be reversed by ADP-ribosylhydrolases. A group of ADP-ribosylhydrolases have a catalytic domain, namely the macrodomain, which is conserved in evolution from prokaryotes to humans. Not all macrodomains remove ADP-ribosylation. One set of macrodomains loses enzymatic activity and only binds to ADP-ribose (ADPR). Here, we summarize the biological functions of these macrodomains in DNA damage repair and compare th...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Yu L, Liu X, Yu X Tags: J Zhejiang Univ Sci B Source Type: research

MRN complex is an essential effector of DNA damage repair.
Abstract Genome stability can be threatened by both endogenous and exogenous agents. Organisms have evolved numerous mechanisms to repair DNA damage, including homologous recombination (HR) and non-homologous end joining (NHEJ). Among the factors associated with DNA repair, the MRE11-RAD50-NBS1 (MRN) complex (MRE11-RAD50-XRS2 in Saccharomyces cerevisiae) plays important roles not only in DNA damage recognition and signaling but also in subsequent HR or NHEJ repair. Upon detecting DNA damage, the MRN complex activates signaling molecules, such as the protein kinase ataxia-telangiectasia mutated (ATM), to trigger a ...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Qiu S, Huang J Tags: J Zhejiang Univ Sci B Source Type: research

Regulation of DNA double-strand break repair pathway choice: a new focus on 53BP1.
Abstract Maintenance of cellular homeostasis and genome integrity is a critical responsibility of DNA double-strand break (DSB) signaling. P53-binding protein 1 (53BP1) plays a critical role in coordinating the DSB repair pathway choice and promotes the non-homologous end-joining (NHEJ)-mediated DSB repair pathway that rejoins DSB ends. New insights have been gained into a basic molecular mechanism that is involved in 53BP1 recruitment to the DNA lesion and how 53BP1 then recruits the DNA break-responsive effectors that promote NHEJ-mediated DSB repair while inhibiting homologous recombination (HR) signaling. This...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Zhang F, Gong Z Tags: J Zhejiang Univ Sci B Source Type: research

DNA alkylation lesion repair: outcomes and implications in cancer chemotherapy.
Abstract Alkylated DNA lesions, induced by both exogenous chemical agents and endogenous metabolites, represent a major form of DNA damage in cells. The repair of alkylation damage is critical in all cells because such damage is cytotoxic and potentially mutagenic. Alkylation chemotherapy is a major therapeutic modality for many tumors, underscoring the importance of the repair pathways in cancer cells. Several different pathways exist for alkylation repair, including base excision and nucleotide excision repair, direct reversal by methyl-guanine methyltransferase (MGMT), and dealkylation by the AlkB homolog (ALKB...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Peng Y, Pei H Tags: J Zhejiang Univ Sci B Source Type: research

Role of deubiquitinating enzymes in DNA double-strand break repair.
Abstract DNA is the hereditary material in humans and almost all other organisms. It is essential for maintaining accurate transmission of genetic information. In the life cycle, DNA replication, cell division, or genome damage, including that caused by endogenous and exogenous agents, may cause DNA aberrations. Of all forms of DNA damage, DNA double-strand breaks (DSBs) are the most serious. If the repair function is defective, DNA damage may cause gene mutation, genome instability, and cell chromosome loss, which in turn can even lead to tumorigenesis. DNA damage can be repaired through multiple mechanisms. Homo...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Li Y, Yuan J Tags: J Zhejiang Univ Sci B Source Type: research

Target binding and residence: a new determinant of DNA double-strand break repair pathway choice in CRISPR/Cas9 genome editing.
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is widely used for targeted genomic and epigenomic modifications and imaging in cells and organisms, and holds tremendous promise in clinical applications. The efficiency and accuracy of the technology are partly determined by the target binding affinity and residence time of Cas9-single-guide RNA (sgRNA) at a given site. However, little attention has been paid to the effect of target binding affinity and residence duration on the repair of Cas9-induced DNA double-strand breaks (DSBs). We propose that...
Source: J Zhejiang Univ Sci ... - January 15, 2021 Category: Science Authors: Feng Y, Liu S, Chen R, Xie A Tags: J Zhejiang Univ Sci B Source Type: research

Hydrogen agronomy: research progress and prospects.
Abstract Agriculture is the foundation of social development. Under the pressure of population growth, natural disasters, environmental pollution, climate change, and food safety, the interdisciplinary "new agriculture" is becoming an important trend of modern agriculture. In fact, new agriculture is not only the foundation of great health and new energy sources, but is also the cornerstone of national food security, energy security, and biosafety. Hydrogen agronomy focuses mainly on the mechanism of hydrogen gas (H2) biology effects in agriculture, and provides a theoretical foundation for the practice ...
Source: J Zhejiang Univ Sci ... - November 1, 2020 Category: Science Authors: Wang YQ, Liu YH, Wang S, Du HM, Shen WB Tags: J Zhejiang Univ Sci B Source Type: research

Application of antigenic biomarkers for Mycobacterium tuberculosis.
e;n JG, Milián-Suazo F Abstract The study and characterization of biomolecules involved in the interaction between mycobacteria and their hosts are crucial to determine their roles in the invasion process and provide basic knowledge about the biology and pathogenesis of disease. Promising new biomarkers for diagnosis and immunotherapy have emerged recently. Mycobacterium is an ancient pathogen that has developed complex strategies for its persistence in the host and environment, likely based on the complexity of the network of interactions between the molecules involved in infection. Several biomarkers have...
Source: J Zhejiang Univ Sci ... - November 1, 2020 Category: Science Authors: Rodríguez-Hernández E, Quintas-Granados LI, Flores-Villalva S, Cantó-Alarcón JG, Milián-Suazo F Tags: J Zhejiang Univ Sci B Source Type: research

Effects of nanofibers on mesenchymal stem cells: environmental factors affecting cell adhesion and osteogenic differentiation and their mechanisms.
Abstract Nanofibers can mimic natural tissue structure by creating a more suitable environment for cells to grow, prompting a wide application of nanofiber materials. In this review, we include relevant studies and characterize the effect of nanofibers on mesenchymal stem cells, as well as factors that affect cell adhesion and osteogenic differentiation. We hypothesize that the process of bone regeneration in vitro is similar to bone formation and healing in vivo, and the closer nanofibers or nanofibrous scaffolds are to natural bone tissue, the better the bone regeneration process will be. In general, cells cultu...
Source: J Zhejiang Univ Sci ... - November 1, 2020 Category: Science Authors: Yu D, Wang J, Qian KJ, Yu J, Zhu HY Tags: J Zhejiang Univ Sci B Source Type: research

Novel ACADVL variants resulting in mitochondrial defects in long-chain acyl-CoA dehydrogenase deficiency.
Abstract The pathogenesis of very-long-chain acyl-CoA dehydrogenase (VLCAD) deficiency is highly heterogeneous and still unclear. Additional novel variants have been recently detected in the population. The molecular and cellular effects of these previously unreported variants are still poorly understood and require further characterization. To address this problem, we have evaluated the various functions and biochemical consequences of six novel missense variants that lead to mild VLCAD deficiency. Marked deficiencies in fatty acid oxidation (FAO) and other mitochondrial defects were observed in cells carrying on...
Source: J Zhejiang Univ Sci ... - November 1, 2020 Category: Science Authors: Chen T, Tong F, Wu XY, Zhu L, Yi QZ, Zheng J, Yang RL, Zhao ZY, Cang XH, Shu Q, Jiang PP Tags: J Zhejiang Univ Sci B Source Type: research

A near-infrared spectroscopy-based end-point determination method for the blending process of Dahuang soda tablets.
CONCLUSIONS: Our results demonstrated that the effect of density plays an important role in improving the performance of the model, and a robust modeling method has been developed. PMID: 33150773 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - November 1, 2020 Category: Science Authors: Wu SJ, Qiu P, Li P, Li Z, Li WL Tags: J Zhejiang Univ Sci B Source Type: research

Diagnostic value of optic disc retinal nerve fiber layer thickness for diabetic peripheral neuropathy.
CONCLUSIONS: The thickness of the RNFL in the optic disc can be used as a diagnostic method for DPN. PMID: 33150774 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - November 1, 2020 Category: Science Authors: Wu XH, Fang JW, Huang YQ, Bai XF, Zhuang Y, Chen XY, Lin XH Tags: J Zhejiang Univ Sci B Source Type: research

Apical ectodermal ridge regulates three principal axes of the developing limb.
Abstract Understanding limb development not only gives insights into the outgrowth and differentiation of the limb, but also has clinical relevance. Limb development begins with two paired limb buds (forelimb and hindlimb buds), which are initially undifferentiated mesenchymal cells tipped with a thickening of the ectoderm, termed the apical ectodermal ridge (AER). As a transitional embryonic structure, the AER undergoes four stages and contributes to multiple axes of limb development through the coordination of signalling centres, feedback loops, and other cell activities by secretory signalling and the activatio...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: Lin GH, Zhang L Tags: J Zhejiang Univ Sci B Source Type: research

Emerging relationship between RNA helicases and autophagy.
Abstract RNA helicases, the largest family of proteins that participate in RNA metabolism, stabilize the intracellular environment through various processes, such as translation and pre-RNA splicing. These proteins are also involved in some diseases, such as cancers and viral diseases. Autophagy, a self-digestive and cytoprotective trafficking process in which superfluous organelles and cellular garbage are degraded to stabilize the internal environment or maintain basic cellular survival, is associated with human diseases. Interestingly, similar to autophagy, RNA helicases play important roles in maintaining cell...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: Zhao MM, Wang RS, Zhou YL, Yang ZG Tags: J Zhejiang Univ Sci B Source Type: research

Verticillin A inhibits colon cancer cell migration and invasion by targeting c-Met.
Abstract Verticillin A is a diketopiperazine compound which was previously isolated from Amanita flavorubescens Alk (containing parasitic fungi Hypomyces hyalines (Schw.) Tul.). Here, we initially found, by wound healing assay and Transwell assay in vitro, that verticillin A possesses an inhibitory effect against the migration and invasion of the human colon cancer cell. Subsequently, c-mesenchymal-epithelial transition factor (c-Met) was identified as a molecular target of verticillin A by screening key genes related to cell migration. Verticillin A-mediated c-Met suppression is at the transcriptional level. Furt...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: Liu QQ, Zeng XL, Guan YL, Lu JX, Tu K, Liu FY Tags: J Zhejiang Univ Sci B Source Type: research

Drying temperature affects rice seed vigor via gibberellin, abscisic acid, and antioxidant enzyme metabolism.
This study aimed to determine the optimum drying temperature for rice seeds according to the IMC, and elucidate the mechanisms mediating the effects of drying temperature and IMC on seed vigor. Rice seeds with three different IMCs (20%, 25%, and 30%) were dried to the target moisture content (14%) at four different drying temperatures. The results showed that the drying temperature and IMC had significant effects on the drying performance and vigor of the rice seeds. The upper limits of drying temperature for rice seeds with 20%, 25%, and 30% IMCs were 45, 42, and 38 °C, respectively. The drying rate and seed temperatu...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: Huang YT, Wu W, Zou WX, Wu HP, Cao DD Tags: J Zhejiang Univ Sci B Source Type: research

Highly sensitive serological approaches for Pepino mosaic virus detection.
In this study, we purified PepMV particles from the infected Nicotiana benthamiana plants and used virions to immunize BALB/c mice to prepare hybridomas secreting anti-PepMV monoclonal antibodies (mAbs). A panel of highly specific and sensitive murine mAbs (15B2, 8H6, 23D11, 20D9, 3A6, and 8E3) could be produced through cell fusion, antibody selection, and cell cloning. Using the mAbs as the detection antibodies, we established double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), Dot-ELISA, and Tissue print-ELISA for detecting PepMV infection in tomato plants. Resulting data on sensitivity analysis assay...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: He WQ, Wu JY, Ren YY, Zhou XP, Zhang SB, Qian YJ, Li FF, Wu JX Tags: J Zhejiang Univ Sci B Source Type: research

Amino acid compounds released by the giant freshwater prawn Macrobrachium rosenbergii during ecdysis: a factor attracting cannibalistic behaviour?
The objective of the present study was to determine the amino acid profile released by M. rosenbergii during the ecdysis process that promotes cannibalism. To accomplish this, changes in amino acid levels (total amino acid (TAA) and free amino acid (FAA)) of tissue muscle, exoskeleton, and sample water of culture medium from the moulting (E-stage) and non-moulting (C-stage) prawns were analysed using high-performance liquid chromatography (HPLC). Comparison study revealed that among the TAA compounds, proline and sarcosine of tissues from moulting prawn were found at the highest levels. The level of FAA from water that con...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: Juneta-Nor AS, Noordin NM, Azra MN, Ma HY, Husin NM, Ikhwanuddin M Tags: J Zhejiang Univ Sci B Source Type: research

Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid.
Abstract The L(+)-form of tartaric acid (L(+)-TA) exists extensively in nature, and is widely used in the food, chemical, textile, building, and pharmaceutical industries (Su et al., 2001). The main method for L(+)-TA production is microbial transformation by cis-epoxysuccinate hydrolase (CESH), which can catalyze the asymmetric hydrolysis of cis-epoxysuccinic acid or its salts to TA or tartrate (Bao et al., 2019). Seventeen species containing CESH have been isolated so far. However, most species for L(+)-TA production have been reported from bacteria (Xuan and Feng, 2019). The only fungus isolated from soil by ou...
Source: J Zhejiang Univ Sci ... - October 1, 2020 Category: Science Authors: Bao WN, Chen Y, Liao HX, Chen H, Liu SW, Liu Y Tags: J Zhejiang Univ Sci B Source Type: research

Roles of miRNA and lncRNA in triple-negative breast cancer.
Abstract Triple-negative breast cancer (TNBC) is currently the most malignant subtype of breast cancer without effective targeted therapies, which makes its pathogenesis an important target for research. A growing number of studies have shown that non-coding RNA (ncRNA), including microRNA (miRNA) and long non-coding RNA (lncRNA), plays a significant role in tumorigenesis. This review summarizes the roles of miRNA and lncRNA in the progression, diagnosis, and neoadjuvant chemotherapy of TNBC. Aberrantly expressed miRNA and lncRNA are listed according to their roles. Further, it describes the multiple mechanisms th...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Xu J, Wu KJ, Jia QJ, Ding XF Tags: J Zhejiang Univ Sci B Source Type: research

MicroRNAs involved in drug resistance of breast cancer by regulating autophagy.
Abstract Autophagy is a conserved catabolic process characterized by degradation and recycling of cytosolic components or organelles through a lysosome-dependent pathway. It has a complex and close relationship to drug resistance in breast cancer. MicroRNAs (miRNAs) are small noncoding molecules that can influence numerous cellular processes including autophagy, through the posttranscriptional regulation of gene expression. Autophagy is regulated by many proteins and pathways, some of which in turn have been found to be regulated by miRNAs. These miRNAs may affect the drug resistance of breast cancer. Drug resista...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Wen N, Lv Q, Du ZG Tags: J Zhejiang Univ Sci B Source Type: research

RGFP966 inactivation of the YAP pathway attenuates cardiac dysfunction induced by prolonged hypothermic preservation.
Abstract Oxidative stress and apoptosis are the key factors that limit the hypothermic preservation time of donor hearts to within 4-6 h. The aim of this study was to investigate whether the histone deacetylase 3 (HDAC3) inhibitor RGFP966 could protect against cardiac injury induced by prolonged hypothermic preservation. Rat hearts were hypothermically preserved in Celsior solution with or without RGFP966 for 12 h followed by 60 min of reperfusion. Hemodynamic parameters during reperfusion were evaluated. The expression and phosphorylation levels of mammalian STE20-like kinase-1 (Mst1) and Yes-associated protein (...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Zheng XH, Wang LL, Zheng MZ, Zhong JJ, Chen YY, Shen YL Tags: J Zhejiang Univ Sci B Source Type: research

Genome-wide identification of the Sec-dependent secretory protease genes in Erwinia amylovora and analysis of their expression during infection of immature pear fruit.
In this study, data mining revealed that E. amylovora harbors all of the essential components of the Sec system. Based on this information, we identified putative Sec-dependent secretory proteases in E. amylovora on a genome-wide scale. Using the programs SignalP, LipoP, and Phobius, a total of 15 putative proteases were predicted to contain the N-terminal signal peptides (SPs) that might link them to the Sec-dependent pathway. The activities of the predicted SPs were further validated using an Escherichia coli-based alkaline phosphatase (PhoA) gene fusion system that confirmed their extracytoplasmic property. Transcriptio...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Zhang WB, Yan HL, Zhu ZC, Zhang C, Du PX, Zhao WJ, Li WM Tags: J Zhejiang Univ Sci B Source Type: research

Early use of dexamethasone increases Nr4a1 in Kupffer cells ameliorating acute liver failure in mice in a glucocorticoid receptor-dependent manner.
CONCLUSIONS: In LPS/D-GaIN-induced ALF mice, early administration of Dex improved ALF by increasing the numbers of innate immune cells, especially Kupffer cells and neutrophils. Gr-dependent Nr4a1 upregulation in Kupffer cells may be an important ALF effect regulated by Dex in this process. PMID: 32893529 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Deng JW, Yang Q, Cai XP, Zhou JM, E WG, An YD, Zheng QX, Hong M, Ren YL, Guan J, Wang G, Lai SJ, Chen Z Tags: J Zhejiang Univ Sci B Source Type: research

Characteristics of chemotherapy-induced diabetes mellitus in acute lymphoblastic leukemia patients.
Abstract Acute lymphocytic leukemia (ALL) is one of the most common malignancies, especially in young people. Combination chemotherapy for ALL typically includes corticosteroids (Kantarjian et al., 2000). Hyperglycemia is a well-recognized complication of corticosteroids, and chemotherapy-induced diabetes (CID) is not uncommon (27.5%-37.0%) during the treatment of ALL (Hsu et al., 2002; Weiser et al., 2004; Alves et al., 2007). Besides the effect of corticosteroids, potential factors triggering hyperglycemia in ALL also include direct infiltration of the pancreas by leukemia cells and β cell dysfunction induc...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Suo SS, Li CY, Zhang Y, Wang JH, Lou YJ, Yu WJ, Jin J Tags: J Zhejiang Univ Sci B Source Type: research

Anti-migratory effects of Piper betle leaf aqueous extract on cancer cells and its microtubule targeting properties.
Abstract Piper betle (PB), also known as "betel" in Malay language, is a tropical Asian vine. PB leaves are commonly chewed by Asians along with betel quid. It contains phenols such as eugenol and hydroxychavicol along with chlorophyll, β-carotene, and vitamin C (Salehi et al., 2019). Extracts from PB leaves have various medicinal properties including anticancer, antioxidant, anti-inflammatory, and antibacterial effects (Salehi et al., 2019). Previous research has shown that PB induces cell cycle arrest at late S or G2/M phase and causes apoptosis at higher doses (Wu et al., 2014; Guha Majumdar and ...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Looi ML, Wong AKH, Gnapragasan SA, Japri AZ, Rajedadram A, Pin KY Tags: J Zhejiang Univ Sci B Source Type: research

Gastrointestinal involvement of COVID-19 and potential faecal transmission of SARS-CoV-2.
Abstract Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was found initially in Wuhan, China in early December 2019. The pandemic has spread to 216 countries and regions, infecting more than 23310 000 people and causing over 800 000 deaths globally by Aug. 24, 2020, according to World Health Organization (https://www.who.int/emergencies/diseases/ novel-coronavirus-2019). Fever, cough, and dyspnea are the three common symptoms of the condition, whereas the conventional transmission route for SARS-CoV-2 is through droplets entering the respiratory trac...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Song M, Li ZL, Zhou YJ, Tian G, Ye T, Zeng ZR, Deng J, Wan H, Li Q, Liu JB Tags: J Zhejiang Univ Sci B Source Type: research

Understanding and reducing the fear of COVID-19.
Abstract The world is now plagued by a pandemic of unprecedented nature caused by a novel, emerging, and still poorly understood infectious disease, coronavirus disease 2019 (COVID-19) (Wu and McGoogan, 2020). In addition to the rapidly growing body of scientific and medical literature that is being published, extensive public reports and stories in both the traditional media and social media have served to generate fear, panic, stigmatization, and instances of xenophobia (Zarocostas, 2020). PMID: 32893533 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Ng KH, Kemp R Tags: J Zhejiang Univ Sci B Source Type: research

Erratum to: Transmission risk of patients with COVID-19 meeting discharge criteria should be interpreted with caution.
Abstract Erratum to: J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2020 21(5):408-410. https://doi.org/10.1631/jzus.B2000117. The original version of this article unfortunately contained a mistake. For Fig. 1a in p.409, the citation of a reference, as well as the permission to reprint this picture, was missing. The correct version and the corresponding reference are given below: (a) Chest computed tomography (CT) image of Patient 1 on admission presents multiple ground-glass opacities distributed in the periphery of inferior lobe of both lungs. Reprinted from Zhang et al. (2020), with kind permission from Spring...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Su JW, Wu WR, Lang GJ, Zhao H, Sheng JF Tags: J Zhejiang Univ Sci B Source Type: research

Retraction Note to: Ambient air pollution and adverse birth outcomes: a systematic review and meta-analysis.
Abstract Retraction Note to: J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2019 20(3):238-252. https://doi.org/10.1631/jzus.B1800122. The authors have retracted this article (Guo et al., 2019) because some data from the original literature had not been converted to appropriate units in the paper, which resulted in deviation of the meta-analysis results. For example, for the forest plot used to examine associations between PM10 exposure and the risk of adverse birth outcomes, the estimates from Brauer et al. (2008), Pedersen et al. (2013), Zhao et al. (2015), and Hansen et al. (2006) were on the originally report...
Source: J Zhejiang Univ Sci ... - August 31, 2020 Category: Science Authors: Guo LQ, Chen Y, Mi BB, Dang SN, Zhao DD, Liu R, Wang HL, Yan H Tags: J Zhejiang Univ Sci B Source Type: research

Therapeutic effect of methane and its mechanism in disease treatment.
Abstract Methane is the simplest hydrocarbon, consisting of one carbon atom and four hydrogen atoms. It is abundant in marsh gas, livestock rumination, and combustible ice. Little is known about the use of methane in human disease treatment. Current research indicates that methane is useful for treating several diseases including ischemia and reperfusion injury, and inflammatory diseases. The mechanisms underlying the protective effects of methane appear primarily to involve anti-oxidation, anti-inflammation, and anti-apoptosis. In this review, we describe the beneficial effects of methane on different diseases, s...
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Ye ZH, Ning K, Ander BP, Sun XJ Tags: J Zhejiang Univ Sci B Source Type: research

Induction of thoracic aortic dissection: a mini-review of β-aminopropionitrile-related mouse models.
Induction of thoracic aortic dissection: a mini-review of β-aminopropionitrile-related mouse models. J Zhejiang Univ Sci B. 2020 Aug.;21(8):603-610 Authors: Zheng HQ, Rong JB, Ye FM, Xu YC, Lu HS, Wang JA Abstract Thoracic aortic dissection (TAD) is one of the most lethal aortic diseases due to its acute onset, rapid progress, and high rate of aortic rupture. The pathogenesis of TAD is not completely understood. In this mini-review, we introduce three emerging experimental mouse TAD models using β-aminopropionitrile (BAPN) alone, BAPN for a prolonged duration (four weeks) and then with added...
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Zheng HQ, Rong JB, Ye FM, Xu YC, Lu HS, Wang JA Tags: J Zhejiang Univ Sci B Source Type: research

Microencapsulation of immunoglobulin Y: optimization with response surface morphology and controlled release during simulated gastrointestinal digestion.
In this study, IgY was microencapsulated by alginate (ALG) and coated with chitooligosaccharide (COS). A response surface methodology was used to optimize the formulation, and a simulated gastrointestinal (GI) digestion (SGID) system to evaluate the controlled release of microencapsulated IgY. The microcapsule formulation was optimized as an ALG concentration of 1.56% (15.6 g/L), COS level of 0.61% (6.1 g/L), and IgY/ALG ratio of 62.44% (mass ratio). The microcapsules prepared following this formulation had an encapsulation efficiency of 65.19%, a loading capacity of 33.75%, and an average particle size of 588.75 μm. U...
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Zhang J, Li HH, Chen YF, Chen LH, Tang HG, Kong FB, Yao YX, Liu XM, Lan Q, Yu XF Tags: J Zhejiang Univ Sci B Source Type: research

Interferon- α2b spray inhalation did not shorten virus shedding time of SARS-CoV-2 in hospitalized patients: a preliminary matched case-control study.
CONCLUSIONS: IFN-α2b spray inhalation did not shorten virus shedding time of SARS-CoV-2 in hospitalized patients. PMID: 32748578 [PubMed - indexed for MEDLINE] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Hao SR, Yan R, Zhang SY, Lian JS, Cai H, Zhang XL, Zheng L, Jia HY, Hu JH, Yu GD, Gu JQ, Ye CY, Jin CL, Lu YF, Xin JJ, Sheng JF, Yang YD Tags: J Zhejiang Univ Sci B Source Type: research

Comparison of the effects of deep and moderate neuromuscular block on respiratory system compliance and surgical space conditions during robot-assisted laparoscopic radical prostatectomy: a randomized clinical study.
CONCLUSIONS: Under the same Pnp pressure in RARP, deep and moderate NMBs resulted in similar changes in Crs, and in other respiratory mechanics and surgical space conditions. However, deep NMB significantly reduced body movements during surgery. PMID: 32748579 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Zhu SJ, Zhang XL, Xie Q, Zhou YF, Wang KR Tags: J Zhejiang Univ Sci B Source Type: research

Lung macrophages are involved in lung injury secondary to repetitive diving.
This study aimed to establish an animal model of decompression-induced lung injury (DILI) secondary to repetitive diving in mice and explore the role of macrophages in DILI and the protective effects of high-concentration hydrogen (HCH) on DILI. Mice were divided into three groups: control group, DILI group, and HCH group. Mice were exposed to hyperbaric air at 600 kPa for 60 min once daily for consecutive 3 d and then experienced decompression. In HCH group, mice were administered with HCH (66.7% hydrogen and 33.3% oxygen) for 60 min after each hyperbaric exposure. Pulmonary function tests were done 6 h after decompressio...
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Ning K, Guan ZB, Lu HT, Zhang N, Sun XJ, Liu WW Tags: J Zhejiang Univ Sci B Source Type: research

New observations on the effect of camellia oil on fatty liver disease in rats.
In this study, the effects of camellia oil, soybean oil, and olive oil on NAFLD were evaluated by analyzing the fatty acid profiles of the plant oils, the serum lipids and lipoproteins of rats fed different oils, and by cytological and ultrastructural observation of the rats' hepatocytes. Analysis of fatty acid profiles showed that the polyunsaturated fatty acid (PUFA) n-6/n-3 ratio was 33.33 in camellia oil, 12.50 in olive oil, and 7.69 in soybean oil. Analyses of serum lipids and lipoproteins of rats showed that the levels of total cholesterol and low-density lipoprotein cholesterol in a camellia oil-fed group (COFG) wer...
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Li CX, Shen LR Tags: J Zhejiang Univ Sci B Source Type: research

Initial chest CT findings in COVID-19: correlation with clinical features.
Abstract In December 2019, coronavirus disease 2019 (COVID-19), a new de novo infectious disease, was first identified in Wuhan, China and quickly spread across China and around the world. The etiology was a novel betacoronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Lu et al., 2020). On Mar. 11, 2020, World Health Organization (WHO) characterized COVID-19 as a global pandemic. As of Mar. 22, 2020, over 292 000 confirmed COVID-19 cases have been reported globally. To date, COVID-19, with its high infectivity, has killed more people than severe acute respiratory syndrome (SARS) and Midd...
Source: J Zhejiang Univ Sci ... - July 31, 2020 Category: Science Authors: Shen ZJ, Lu N, Gao LL, Lv J, Luo HF, Jiang JF, Xu C, Li SY, Mao JJ, Li K, Xu XP, Lin B Tags: J Zhejiang Univ Sci B Source Type: research

Epigenetic role of N6-methyladenosine (m6A) RNA methylation in the cardiovascular system.
Abstract As the most prevalent and abundant transcriptional modification in the eukaryotic genome, the continuous and dynamic regulation of N6-methyladenosine (m6A) has been shown to play a vital role in physiological and pathological processes of cardiovascular diseases (CVDs), such as ischemic heart failure (HF), myocardial hypertrophy, myocardial infarction (MI), and cardiomyogenesis. Regulation is achieved by modulating the expression of m6A enzymes and their downstream cardiac genes. In addition, this process has a major impact on different aspects of internal biological metabolism and several other external ...
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Zhao K, Yang CX, Li P, Sun W, Kong XQ Tags: J Zhejiang Univ Sci B Source Type: research

Biological pacemaker: from biological experiments to computational simulation.
Abstract Pacemaking dysfunction has become a significant disease that may contribute to heart rhythm disorders, syncope, and even death. Up to now, the best way to treat it is to implant electronic pacemakers. However, these have many disadvantages such as limited battery life, infection, and fixed pacing rate. There is an urgent need for a biological pacemaker (bio-pacemaker). This is expected to replace electronic devices because of its low risk of complications and the ability to respond to emotion. Here we survey the contemporary development of the bio-pacemaker by both experimental and computational approache...
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Li Y, Wang K, Li Q, Zhang H Tags: J Zhejiang Univ Sci B Source Type: research

Multi-biomarker strategy for prediction of myocardial dysfunction and mortality in sepsis.
CONCLUSIONS: The findings of this study indicate that a sensitive and specific strategy for early diagnosis of SIMD and mortality prediction in sepsis should incorporate three biomarkers. PMID: 32633108 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Chen FC, Xu YC, Zhang ZC Tags: J Zhejiang Univ Sci B Source Type: research

Role of adjuvant (chemo)radiotherapy for resected extrahepatic cholangiocarcinoma: a meta-analysis.
Abstract BACKGROUND: Adjuvant (chemo)radiotherapy (A(C)RT) may be an important supplement to surgery for extrahepatic cholangiocarcinoma (EHCC). However, whether all patients would achieve benefits from A(C)RT and which adjuvant regimen, adjuvant radiotherapy (ART) or adjuvant chemoradiotherapy (ACRT), would be preferred, are still undetermined. The low incidence of EHCC makes it difficult to carry out randomized controlled trials (RCTs); therefore, almost all clinical studies on radiotherapy are retrospective. We have conducted a meta-analysis of these retrospective studies. METHODS: We conducted a meta-anal...
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Shi XQ, Zhang JY, Tian H, Tang LN, Li AL Tags: J Zhejiang Univ Sci B Source Type: research

Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin.
Abstract Porcine circovirus 3 (PCV3) has been detected in major pig-producing countries around the world since its first report in the US in 2016. Most current studies have focused on epidemiological investigations and detection methods of PCV3 because of lack of live virus strains for research on its pathogenesis in porcine cells or even in pigs. We constructed a recombinant plasmid pCMV-Cap carrying the PCV3 orf2 gene to investigate the effects of capsid (Cap) protein expression on autophagic response in human embryonic kidney cell line 293T (HEK293T). We demonstrate that PCV3 Cap protein induced complete autoph...
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Geng SC, Li XL, Fang WH Tags: J Zhejiang Univ Sci B Source Type: research

Hyaluronic acid derivative-modified nano-structured lipid carrier for cancer targeting and therapy.
Abstract To reduce the problems of poor solubility, high in vivo dosage requirement, and weak targeting ability of paclitaxel (PTX), a hyaluronic acid-octadecylamine (HA-ODA)-modified nano-structured lipid carrier (HA-NLC) was constructed. HA-ODA conjugates were synthesized by an amide reaction between HA and ODA. The hydrophobic chain of HA-ODA can be embedded in the lipid core of the NLC to obtain HA-NLC. The HA-NLC displayed strong internalization in cluster determinant 44 (CD44) highly expressed MCF-7 cells, and endocytosis mediated by the CD44 receptor was involved. The HA-NLC had an encapsulation efficiency ...
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Liu X, Liu H, Wang SL, Liu JW Tags: J Zhejiang Univ Sci B Source Type: research

Postprandial proximal gastric acid pocket and its association with gastroesophageal acid reflux in patients with short-segment Barrett's esophagus.
CONCLUSIONS: In patients with short-segment BE, a PPGAP is commonly seen. Its length and acidity of PPGAP are associated with gastroesophageal acid reflux, the DeMeester score, and the GerdQ score in patients with short-segment BE. PMID: 32633112 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Nian YY, Meng XM, Wu J, Jing FC, Wang XQ, Dang T, Zhang J Tags: J Zhejiang Univ Sci B Source Type: research

Secondary manifestations of mitochondrial disorders.
Abstract Mitochondrial disorders (MIDs) are a heterogeneous group of genetic metabolic diseases due to mutations in the mitochondrial DNA (mtDNA) or in the nuclear DNA (nDNA) (Rahman and Rahman, 2018). Some affected genes encode proteins with various functions, or structural RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs). MIDs may also be caused by mutations in non-coding regions (e.g., D-loop of mtDNA) (Rahman and Rahman, 2018). Proteins involved in MIDs include enzymes, assembling factors, transport proteins, signaling proteins, pore proteins, and fusion/fission proteins (Gorman et al., 2016). The...
Source: J Zhejiang Univ Sci ... - June 30, 2020 Category: Science Authors: Finsterer J Tags: J Zhejiang Univ Sci B Source Type: research

Breeding crops by design for future agriculture.
Abstract Plant breeding is both the science and art of developing elite crop cultivars by creating and reassembling desirable inherited traits for human benefit. From the bulk selection of wild plants for cultivation during early civilization to Mendelian genetics and genomics-assisted breeding in modern society, breeding methodologies have evolved over the last thousand years. In the past few decades, the "Green Revolution" through breeding of semi-dwarf wheat and rice varieties, and the use of heterosis and transgenic crops have dramatically enhanced crop productivity and helped prevent widespread fami...
Source: J Zhejiang Univ Sci ... - May 31, 2020 Category: Science Authors: Li C Tags: J Zhejiang Univ Sci B Source Type: research

Advances in studies on ion transporters involved in salt tolerance and breeding crop cultivars with high salt tolerance.
Abstract Soil salinity is a global major abiotic stress threatening crop productivity. In salty conditions, plants may suffer from osmotic, ionic, and oxidative stresses, resulting in inhibition of growth and development. To deal with these stresses, plants have developed a series of tolerance mechanisms, including osmotic adjustment through accumulating compatible solutes in the cytoplasm, reactive oxygen species (ROS) scavenging through enhancing the activity of anti-oxidative enzymes, and Na+/K+ homeostasis regulation through controlling Na+ uptake and transportation. In this review, recent advances in studies ...
Source: J Zhejiang Univ Sci ... - May 31, 2020 Category: Science Authors: Huang L, Wu DZ, Zhang GP Tags: J Zhejiang Univ Sci B Source Type: research

Breeding for low cadmium accumulation cereals.
Abstract Cadmium (Cd) is an element that is nonessential and extremely toxic to both plants and human beings. Soil contaminated with Cd has adverse impacts on crop yields and threatens human health via the food chain. Cultivation of low-Cd cultivars has been of particular interest and is one of the most cost-effective and promising approaches to minimize human dietary intake of Cd. Low-Cd crop cultivars should meet particular criteria, including acceptable yield and quality, and their edible parts should have Cd concentrations below maximum permissible concentrations for safe consumption, even when grown in Cd-con...
Source: J Zhejiang Univ Sci ... - May 31, 2020 Category: Science Authors: Chen Q, Wu FB Tags: J Zhejiang Univ Sci B Source Type: research