An overview of COVID-19.
Abstract Pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection emerged in Wuhan City, Hubei Province, China in December 2019. By Feb. 11, 2020, the World Health Organization (WHO) officially named the disease resulting from infection with SARS-CoV-2 as coronavirus disease 2019 (COVID-19). COVID-19 represents a spectrum of clinical manifestations that typically include fever, dry cough, and fatigue, often with pulmonary involvement. SARS-CoV-2 is highly contagious and most individuals within the population at large are susceptible to infection. Wild animal hosts and infected pat...
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Shi Y, Wang G, Cai XP, Deng JW, Zheng L, Zhu HH, Zheng M, Yang B, Chen Z Tags: J Zhejiang Univ Sci B Source Type: research

Possible aerosol transmission of COVID-19 and special precautions in dentistry.
Abstract Since its emergence in December 2019, corona virus disease 2019 (COVID-19) has impacted several countries, affecting more than 90 thousand patients and making it a global public threat. The routes of transmission are direct contact, and droplet and possible aerosol transmissions. Due to the unique nature of dentistry, most dental procedures generate significant amounts of droplets and aerosols, posing potential risks of infection transmission. Understanding the significance of aerosol transmission and its implications in dentistry can facilitate the identification and correction of negligence in daily den...
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Ge ZY, Yang LM, Xia JJ, Fu XH, Zhang YZ Tags: J Zhejiang Univ Sci B Source Type: research

Comparison of epidemiological and clinical characteristics of COVID-19 patients with and without Wuhan exposure history in Zhejiang Province, China.
This study investigates the epidemiological and clinical characteristics of coronavirus disease 2019 (COVID-19) patients in Zhejiang Province who did or did not have a history of Wuhan exposure. METHODS: We collected data from medical records of confirmed COVID-19 patients in Zhejiang Province from Jan. 17 to Feb. 7, 2020 and analyzed epidemiological, clinical, and treatment data of those with and without recorded recent exposure in Wuhan. RESULTS: Patients in the control group were older than those in the exposure group ((48.19±16.13) years vs. (43.47±13.12) years, P
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Lian JS, Cai H, Hao SR, Jin X, Zhang XL, Zheng L, Jia HY, Hu JH, Zhang SY, Yu GD, Gu JQ, Ye CY, Jin CL, Lu YF, Sheng JF, Yang YD Tags: J Zhejiang Univ Sci B Source Type: research

Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China.
CONCLUSIONS: Data showed that the rates of complications, dynamics of lymphocytopenia, and changes in levels of platelet, hemoglobin, BUN, D-dimer, LDH and IL-6, and prothrombin time in these ICU patients were significantly different between IMV and NIV cases. PMID: 32425003 [PubMed - indexed for MEDLINE] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Zheng Y, Sun LJ, Xu M, Pan J, Zhang YT, Fang XL, Fang Q, Cai HL Tags: J Zhejiang Univ Sci B Source Type: research

Clinical value of the emergency department in screening and diagnosis of COVID-19 in China.
Abstract Since the global outbreak of severe acute respiratory syndrome (SARS) in 2003, China has gradually built a robust prevention and control system for sudden infectious diseases. All large hospitals have a fever clinic that isolates patients with all kinds of acute communicable diseases as the first line of medical defense. The emergency department, as the second line of medical defense in hospitals, is constantly shouldering the heavy responsibility of screening communicable diseases while also treating all kinds of other non-communicable acute and critical diseases (Zhang et al., 2012; Zhu et al., 2015; Wa...
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Zhang Q, Pan J, Zhao MX, Lu YQ Tags: J Zhejiang Univ Sci B Source Type: research

Dialectical behavior therapy-based psychological intervention for woman in late pregnancy and early postpartum suffering from COVID-19: a case report.
Abstract At the end of 2019, a new form of pneumonia disease known as the corona virus disease 2019 (COVID-19) rapidly spread throughout most provinces of China, and the total global number of COVID-19 cases has surpassed 500 000 by Mar. 27, 2020 (WHO, 2020). On Jan. 30, 2020, the World Health Organization (WHO) declared COVID-19 a global health emergency (WHO, 2020). COVID-19 causes most damage to the respiratory system, leading to pneumonia or breathing difficulties. The confirmed case fatality risk (cCFR) was estimated to be 5% to 8% (Jung et al., 2020). Besides physical pain, COVID-19 also induces psychologica...
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Huang JW, Zhou XY, Lu SJ, Xu Y, Hu JB, Huang ML, Wang HF, Hu CC, Li SG, Chen JK, Wang Z, Hu SH, Wei N Tags: J Zhejiang Univ Sci B Source Type: research

Efficacy of internet-based integrated intervention on depression and anxiety symptoms in patients with COVID-19.
Abstract Public health crises, such as the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) since Dec. 2019, are widely acknowledged as severe traumatic events that impose threats not only because of physical concerns but also because of the psychological distress of infected patients. We designed an internet-based integrated intervention and evaluated its efficacy on depression and anxiety symptoms in patients infected by SARS-CoV-2. PMID: 32425006 [PubMed - indexed for MEDLINE] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Wei N, Huang BC, Lu SJ, Hu JB, Zhou XY, Hu CC, Chen JK, Huang JW, Li SG, Wang Z, Wang DD, Xu Y, Hu SH Tags: J Zhejiang Univ Sci B Source Type: research

Can SARS-CoV-2-infected women breastfeed after viral clearance?
Abstract The recently emerged novel coronavirus pneumonia, named the coronavirus disease 2019 (COVID-19), shares several clinical characteristics with severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), and spread rapidly throughout China in December of 2019 (Huang et al., 2020). The pathogen 2019 novel coronavirus (2019-nCoV) is now named SARS coronavirus 2 (SARS-CoV-2) and is highly infectious. As of Apr. 9, 2020, over 80 000 confirmed cases had been reported, with an estimated mortality rate of 4.0% (Chinese Center for Disease Control and Prevention, 2020). Person-to-person tra...
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Lang GJ, Zhao H Tags: J Zhejiang Univ Sci B Source Type: research

Transmission risk of patients with COVID-19 meeting discharge criteria should be interpreted with caution.
Abstract As of Apr. 22, 2020, the World Health Organization (2020) has reported over 2.4 million confirmed coronavirus disease 2019 (COVID-19) patients and 169 151 deaths. Recent articles have uncovered genomic characteristics and clinical features of COVID-19 (Chan et al., 2020; Chang et al., 2020; Guan et al., 2020; Zhu et al., 2020), while our understanding of COVID-19 is still limited. As suggested by guidelines promoted by the General Office of National Health Commission of the People's Republic of China (2020) (from Versions 1 to 6), discharged standards for COVID-19 were still dependent on viral real-time p...
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Su JW, Wu WR, Lang GJ, Zhao H, Sheng JF Tags: J Zhejiang Univ Sci B Source Type: research

Primary percutaneous coronary intervention in a COVID-19 patient with ST-segment elevation myocardial infarction after lung transplantation: a case report.
We present an unusual case of a patient with bilateral-lung transplantation due to severe coronavirus disease 2019 (COVID-19), who subsequently suffered complications with acute myocardial infarction and underwent primary percutaneous coronary intervention (PCI). PMID: 32425009 [PubMed - indexed for MEDLINE] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Xuan TM, Wang XX, Pu XY, Han WL, Guo XG Tags: J Zhejiang Univ Sci B Source Type: research

Analysis of an improved workflow of endoscope reprocessing for bedside endoscopic diagnosis and treatment on COVID-19 patients.
Abstract Severe cases infected with the coronavirus disease 2019 (COVID-19), named by the World Health Organization (WHO) on Feb. 11, 2020, tend to present a hypercatabolic state because of severe systemic consumption, and are susceptible to stress ulcers and even life-threatening gastrointestinal bleeding. Endoscopic diagnosis and treatment constitute an irreplaceable part in the handling of severe COVID-19 cases. Endoscopes, as reusable precision instruments with complicated structures, require more techniques than other medical devices in cleaning, disinfection, sterilization, and other reprocessing procedures....
Source: J Zhejiang Univ Sci ... - May 1, 2020 Category: Science Authors: Gu Q, Wang HF, Fang Y, Lu Y, Shen Z, Wang Y, Wu X, Cen L, Chen YS Tags: J Zhejiang Univ Sci B Source Type: research

Effects of sulforaphane on brain mitochondria: mechanistic view and future directions.
Abstract The organosulfur compound sulforaphane (SFN; C6H11NOS2) is a potent cytoprotective agent promoting antioxidant, anti-inflammatory, antiglycative, and antimicrobial effects in in vitro and in vivo experimental models. Mitochondria are the major site of adenosine triphosphate (ATP) production due to the work of the oxidative phosphorylation (OXPHOS) system. They are also the main site of reactive oxygen species (ROS) production in nucleated human cells. Mitochondrial impairment is central in several human diseases, including neurodegeneration and metabolic disorders. In this paper, we describe and discuss t...
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Jardim FR, Almeida FJS, Luckachaki MD, Oliveira MR Tags: J Zhejiang Univ Sci B Source Type: research

Use of Tripterygium wilfordii Hook F for immune-mediated inflammatory diseases: progress and future prospects.
Abstract Tripterygium wilfordii Hook F has significant anti-inflammatory and immunosuppressive properties and is widely used for treating autoimmune and inflammatory diseases such as rheumatoid arthritis, systemic lupus erythematosus, and kidney disease, especially in traditional Chinese medicine. The mechanisms underlying its effects may be diverse but they remain unclear, and its toxicity and side effects limit its wider clinical application. This review summarizes the clinical application of Tripterygium wilfordii Hook F in recent years, as well as the results of studies into its mechanisms and toxicity, to pro...
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Song CY, Xu YG, Lu YQ Tags: J Zhejiang Univ Sci B Source Type: research

Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology.
CONCLUSIONS: The data from this study have built a foundation to develop many hypotheses to further explore the hypoxia/re-oxygenation mechanisms, an area with great clinical significance for multiple diseases. PMID: 32253839 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Xu J, Jiang JK, Li XL, Yu XP, Xu YG, Lu YQ Tags: J Zhejiang Univ Sci B Source Type: research

Central visual function and inner retinal structure in primary open-angle glaucoma.
This study enrolled 78 POAG patients and 58 healthy controls. POAG was classified into early glaucoma and moderate to advanced glaucoma. The following tests were performed on all participants: isolated-check visual evoked potential (icVEP) testing, 24-2 standard automated perimetry (SAP), and Cirrus optical coherence tomography (OCT) examinations. Signal-to-noise ratio (SNR) measures obtained from icVEP responses to isolated checks presented at four depths of modulation (DOMs; 8%, 14%, 22%, and 32%) were explored. Mean macular sensitivity (mMS) was assessed by calculating the mean sensitivities of central 12 SAP points. Ga...
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Xu LJ, Li SL, Zemon V, Xie YQ, Liang YB Tags: J Zhejiang Univ Sci B Source Type: research

Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition.
CONCLUSIONS: COX-2 promotes cancer cell migration and CDDP resistance and may serve as a potential target for curing OC. PMID: 32253841 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Deng L, Feng DQ, Ling B Tags: J Zhejiang Univ Sci B Source Type: research

Effects of leptin-modified human placenta-derived mesenchymal stem cells on angiogenic potential and peripheral inflammation of human umbilical vein endothelial cells (HUVECs) after X-ray radiation.
Abstract Combined radiation-wound injury (CRWI) is characterized by blood vessel damage and pro-inflammatory cytokine deficiency. Studies have identified that the direct application of leptin plays a significant role in angiogenesis and inflammation. We established a sustained and stable leptin expression system to study the mechanism. A lentivirus method was employed to explore the angiogenic potential and peripheral inflammation of irradiated human umbilical vein endothelial cells (HUVECs). Leptin was transfected into human placenta-derived mesenchymal stem cells (HPMSCs) with lentiviral vectors. HUVECs were irr...
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Chen S, Wang Q, Han B, Wu J, Liu DK, Zou JD, Wang M, Liu ZH Tags: J Zhejiang Univ Sci B Source Type: research

Erratum to: Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF- κB signaling.
Erratum to: Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling. J Zhejiang Univ Sci B. 2020 Apr.;21(4):341 Authors: Zhang H, Wu ZM, Yang YP, Shaukat A, Yang J, Guo YF, Zhang T, Zhu XY, Qiu JX, Deng GZ, Shi DM Abstract Erratum to: J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2019 2019 20(10):816-827. https://doi.org/10.1631/jzus.B1900071. The original version of this article unfortunately contained a mistake. In p.823, Figs. 8c and 8d were in-correct, and the obvious pathological changes were mistakenly placed in the picture. The correct versions ...
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Zhang H, Wu ZM, Yang YP, Shaukat A, Yang J, Guo YF, Zhang T, Zhu XY, Qiu JX, Deng GZ, Shi DM Tags: J Zhejiang Univ Sci B Source Type: research

Retraction Note to: Effects of dietary glycerol monolaurate on productive performance, egg quality, serum biochemical indices, and intestinal morphology of laying hens.
Abstract Retraction Note to: J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2019 20(11):877-890. https://doi.org/10.1631/jzus.B1800530. The authors have retracted this article (Zhao et al., 2019) due to significant overlap with a previously published Chinese language article (Liu et al., 2017), including overlap in Table 1, Table 2, Table 3, Table 6, Fig. 4, and part of the results (Sections 3.1, 3.2, 3.5, and 3.7). All authors agree with this retraction. PMID: 32253844 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - April 1, 2020 Category: Science Authors: Zhao MJ, Cai HY, Liu MY, Deng LL, Li Y, Zhang H, Feng FQ Tags: J Zhejiang Univ Sci B Source Type: research

Role of LINC00152 in non-small cell lung cancer.
Abstract Non-small cell lung cancer (NSCLC) accounts for about 85% of all lung cancer cases. The pathogenesis of NSCLC involves complex gene networks that include different types of non-coding RNAs, such as long non-coding RNAs (lncRNAs). The role of lncRNAs in NSCLC is gaining an increasing interest as their function is being explored in various human cancers. Recently, a new oncogenic lncRNA, LINC00152 (cytoskeleton regulator RNA (CYTOR)), has been identified in different tumor types. In NSCLC, the high expression of LINC00152 in tumor tissue and peripheral blood samples has been shown to be associated with wors...
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Yu H, Li SB Tags: J Zhejiang Univ Sci B Source Type: research

Roles of pattern recognition receptors in diabetic nephropathy.
Abstract Diabetic nephropathy (DN) is currently the most common complication of diabetes. It is considered to be one of the leading causes of end-stage renal disease (ESRD) and affects many diabetic patients. The pathogenesis of DN is extremely complex and has not yet been clarified; however, in recent years, increasing evidence has shown the important role of innate immunity in DN pathogenesis. Pattern recognition receptors (PRRs) are important components of the innate immune system and have a significant impact on the occurrence and development of DN. In this review, we classify PRRs into secretory, endocytic, a...
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Zhou ZF, Jiang L, Zhao Q, Wang Y, Zhou J, Chen QK, Lv JL Tags: J Zhejiang Univ Sci B Source Type: research

Regulatory mechanisms and therapeutic potential of microglial inhibitors in neuropathic pain and morphine tolerance.
Abstract Microglia are important cells involved in the regulation of neuropathic pain (NPP) and morphine tolerance. Information on their plasticity and polarity has been elucidated after determining their physiological structure, but there is still much to learn about the role of this type of cell in NPP and morphine tolerance. Microglia mediate multiple functions in health and disease by controlling damage in the central nervous system (CNS) and endogenous immune responses to disease. Microglial activation can result in altered opioid system activity, and NPP is characterized by resistance to morphine. Here we in...
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Du ER, Fan RP, Rong LL, Xie Z, Xu CS Tags: J Zhejiang Univ Sci B Source Type: research

Inhibition of chemotherapy-related breast tumor EMT by application of redox-sensitive siRNA delivery system CSO-ss-SA/siRNA along with doxorubicin treatment.
Abstract Metastasis is one of the main reasons causing death in cancer patients. It was reported that chemotherapy might induce metastasis. In order to uncover the mechanism of chemotherapy-induced metastasis and find solutions to inhibit treatment-induced metastasis, the relationship between epithelial-mesenchymal transition (EMT) and doxorubicin (DOX) treatment was investigated and a redox-sensitive small interfering RNA (siRNA) delivery system was designed. DOX-related reactive oxygen species (ROS) were found to be responsible for the invasiveness of tumor cells in vitro, causing enhanced EMT and cytoskeleton r...
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Liu X, Zhou XQ, Shang XW, Wang L, Li Y, Yuan H, Hu FQ Tags: J Zhejiang Univ Sci B Source Type: research

Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway.
Abstract Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality globally. It accounts for the majority of primary liver cancer cases. Amyloid precursor protein (APP), a cell membrane protein, plays a vital role in the pathogenesis of Alzheimer's disease, and has been found to be implicated in tumor growth and metastasis. Therefore, to understand the relationship between APP and 5-fluorouracil (5-FU) resistance in liver cancer, Cell Counting Kit-8, apoptosis and cell cycle assays, western blotting, and reverse transcription-quantitative polymerase chain reaction (qPCR) analysis were p...
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Wu XL, Chen Y, Kong WC, Zhao ZQ Tags: J Zhejiang Univ Sci B Source Type: research

Identification of PTPRR and JAG1 as key genes in castration-resistant prostate cancer by integrated bioinformatics methods.
In conclusion, PTPRR and JAG1 are key genes in the CRPC, which may serve as promising biomarkers of diagnosis and prognosis of CRPC. PMID: 32133801 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Wang JL, Wang Y, Ren GP Tags: J Zhejiang Univ Sci B Source Type: research

Primary age-related tauopathy in a Chinese cohort.
Abstract Primary age-related tauopathy (PART) is characterized by the presence of tau neurofibrillary tangles (NFTs) which are typically observed in Alzheimer's disease (AD) brains, with few or without β-amyloid (Aβ) plaques. The diagnosis of PART can be categorized into "definite" or "possible" depending on the amount of Aβ plaques. Definite PART is diagnosed when NFTs are observed and the Braak stage is ≤IV, with Thal Aβ Phase 0 (Crary et al., 2014). According to the neuropathological diagnostic criteria, we reported that PART was frequently observed in the Chinese popu...
Source: J Zhejiang Univ Sci ... - March 1, 2020 Category: Science Authors: Wang X, Zhang L, Lu H, Wu JL, Liang HZ, Liu C, Tao QQ, Wu ZY, Zhu KQ Tags: J Zhejiang Univ Sci B Source Type: research

Salinity tolerance in barley during germination- homologs and potential genes.
Abstract Salinity affects more than 6% of the world's total land area, causing massive losses in crop yield. Salinity inhibits plant growth and development through osmotic and ionic stresses; however, some plants exhibit adaptations through osmotic regulation, exclusion, and translocation of accumulated Na+ or Cl-. Currently, there are no practical, economically viable methods for managing salinity, so the best practice is to grow crops with improved tolerance. Germination is the stage in a plant's life cycle most adversely affected by salinity. Barley, the fourth most important cereal crop in the world, has outst...
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Mwando E, Angessa TT, Han Y, Li C Tags: J Zhejiang Univ Sci B Source Type: research

Roles of PTBP1 in alternative splicing, glycolysis, and oncogensis.
Abstract Polypyrimidine tract-binding protein 1 (PTBP1) plays an essential role in splicing and is expressed in almost all cell types in humans, unlike the other proteins of the PTBP family. PTBP1 mediates several cellular processes in certain types of cells, including the growth and differentiation of neuronal cells and activation of immune cells. Its function is regulated by various molecules, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and RNA-binding proteins. PTBP1 plays roles in various diseases, particularly in some cancers, including colorectal cancer, renal cell cancer, breast cancer, an...
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Zhu W, Zhou BL, Rong LJ, Ye L, Xu HJ, Zhou Y, Yan XJ, Liu WD, Zhu B, Wang L, Jiang XJ, Ren CP Tags: J Zhejiang Univ Sci B Source Type: research

Preliminary study on the role of novel LysR family gene kp05372 in Klebsiella pneumoniae of forest musk deer.
Abstract LysR-type transcriptional regulators are involved in the regulation of numerous cellular metabolic processes in Klebsiella pneumoniae, leading to severe infection. Earlier, we found a novel LysR family gene, named kp05372, in a strain of K. pneumoniae (designated GPKP) isolated from forest musk deer. To study the function of this gene in relation to the biological characteristics of GPKP, we used the suicide plasmid and conjugative transfer methods to construct deletion mutant strain GPKP-Δkp05372; moreover, we also constructed the GPKP-Δkp05372+ complemented strain. The role of this gene was ...
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Yang W, Wang WY, Zhao W, Cheng JG, Wang Y, Yao XP, Yang ZX, Yu D, Luo Y Tags: J Zhejiang Univ Sci B Source Type: research

Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice.
Abstract Painful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated P2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was a...
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Ni CM, Sun HP, Xu X, Ling BY, Jin H, Zhang YQ, Zhao ZQ, Cao H, Xu L Tags: J Zhejiang Univ Sci B Source Type: research

CX3CR1 contributes to streptozotocin-induced mechanical allodynia in the mouse spinal cord.
Abstract Patients with diabetic peripheral neuropathy experience debilitating pain that significantly affects their quality of life (Abbott et al., 2011), by causing sleeping disorders, anxiety, and depression (Dermanovic Dobrota et al., 2014). The primary clinical manifestation of painful diabetic neuropathy (PDN) is mechanical hypersensitivity, also known as mechanical allodynia (MA) (Callaghan et al., 2012). MA's underlying mechanism remains poorly understood, and so far, based on symptomatic treatment, it has no effective therapy (Moore et al., 2014). PMID: 32115913 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Ni CM, Ling BY, Xu X, Sun HP, Jin H, Zhang YQ, Cao H, Xu L Tags: J Zhejiang Univ Sci B Source Type: research

Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS.
Abstract Blakeslea trispora is a natural source of carotenoids, including β-carotene and lycopene, which have industrial applications. Therefore, classical selective breeding techniques have been applied to generate strains with increased productivity, and microencapsulated β-carotene preparation has been used in food industry (Li et al., 2019). In B. trispora, lycopene is synthesized via the mevalonate pathway (Venkateshwaran et al., 2015). Lycopene cyclase, which is one of the key enzymes in this pathway, is a bifunctional enzyme that can catalyze the cyclization of lycopene to produce β-carotene ...
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Liu Y, Shao YR, Li XY, Wang ZM, Yang LR, Zhang YZ, Wu MB, Yao JM Tags: J Zhejiang Univ Sci B Source Type: research

Erratum to: Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation.
Abstract Erratum to: J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2019 20(7):605-612. https://doi.org/10.1631/jzus.B1900051. The original version of this article unfortunately contained a mistake. In p.605, the number of the Zhejiang Provincial Natural Science Foundation of China (No. Y17H160118) in Funding is incorrect. The correct number should be LY17H160026, which is the approval number of the project, whereas Y17H160118 is the application number of the project. PMID: 32115915 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - February 1, 2020 Category: Science Authors: Zhang AB, Peng YF, Jia JJ, Nie Y, Zhang SY, Xie HY, Zhou L, Zheng SS Tags: J Zhejiang Univ Sci B Source Type: research

Lymphatic vessels, miRNAs, and CAR T cells in tumor immunology.
Abstract This special feature contains three review articles that summarize recent advances pertaining to tumor immunobiology. Normalization of antitumor immunity through checkpoint inhibitors has achieved significant clinical success and benefited many cancer patients. However, not all cancer patients respond to these treatments, and among the responders, some may develop resistance and others may suffer autoimmunity that requires intervention. Tumor immunotherapy holds promise for further improving the survival of cancer patients, but deeper understanding of immunological networks that regulate anti- and pro-tum...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Jiang X Tags: J Zhejiang Univ Sci B Source Type: research

MicroRNAs in tumor immunity: functional regulation in tumor-associated macrophages.
Abstract Tumor-associated macrophages (TAMs) are the most abundant immune cells in the tumor microenvironment (TME) and are critical for cancer initiation and progression. MicroRNAs (miRNAs) could notably influence the phenotype of TAMs through various targets and signal pathways during cancer progression due to their post-transcriptional regulation. In this review, we discuss mainly the regulatory function of miRNAs on macrophage differentiation, functional polarization, and cellular crosstalk. Firstly, during the generation process, miRNAs take part in the differentiation from myeloid cells to mature macrophages...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Chen C, Liu JM, Luo YP Tags: J Zhejiang Univ Sci B Source Type: research

Current advances in chimeric antigen receptor T-cell therapy for refractory/relapsed multiple myeloma.
Abstract Multiple myeloma (MM), considered an incurable hematological malignancy, is characterized by its clonal evolution of malignant plasma cells. Although the application of autologous stem cell transplantation (ASCT) and the introduction of novel agents such as immunomodulatory drugs (IMiDs) and proteasome inhibitors (PIs) have doubled the median overall survival to eight years, relapsed and refractory diseases are still frequent events in the course of MM. To achieve a durable and deep remission, immunotherapy modalities have been developed for relapsed/refractory multiple myeloma (RRMM). Among these approac...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Huang H, Wu HW, Hu YX Tags: J Zhejiang Univ Sci B Source Type: research

Relationships between blood leukocyte mitochondrial DNA copy number and inflammatory cytokines in knee osteoarthritis.
Abstract Osteoarthritis (OA) is a degenerative articular disorder manifested by cartilage destruction, subchondral sclerosis, osteophytes, and synovitis, resulting in chronic joint pain and physical disability in the elderly. The purpose of this study was to investigate mitochondrial DNA copy number (mtDNACN) and inflammatory cytokines in primary knee OA patients and healthy volunteers. A total of 204 knee OA patients and 169 age-matched healthy volunteers were recruited. Their relative blood leukocyte mtDNACN was assessed by quantitative real-time polymerase chain reaction (qRT-PCR), and ten inflammatory cytokine...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Zhan D, Tanavalee A, Tantavisut S, Ngarmukos S, Edwards SW, Honsawek S Tags: J Zhejiang Univ Sci B Source Type: research

Psychophysiological and cognitive effects of strawberry plants on people in isolated environments.
This study further explored the impact of viewing strawberry plants on cognition. A notable reduction of the subjects' reaction time after 15-min plant viewing was observed. Based on these findings, a long-duration isolated experiment in a bioregenerative life support system-"Lunar Palace I"-was conducted. A similar trend was obtained that crew members' mood states were improved by viewing the strawberry plants, but no significant change was observed. This study provided some experimental evidence for the benefits of interacting with strawberry plants in isolated environments. PMID: 31898442 [PubMed - in pro...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Li ZM, Liu H, Zhang WZ, Liu H Tags: J Zhejiang Univ Sci B Source Type: research

Carfilzomib inhibits the growth of lung adenocarcinoma via upregulation of Gadd45a expression.
In this study, we have systematically investigated the CFZ functions in cell proliferation and growth, cell cycle arrest, and apoptosis in lung adenocarcinoma cells. Flow cytometry experiments showed that CFZ significantly induced G2/M cell cycle arrest and apoptosis in lung adenocarcinoma. MTS and colony formation assays revealed that CFZ substantially inhibited survival of lung adenocarcinoma cells. All results were consistently correlated to the upregulation expression of Gadd45a, which is an important gene in modulating cell cycle arrest and apoptosis in response to physiologic and environmental stresses. Here, upregul...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Yang F, Liu WW, Chen H, Zhu J, Huang AH, Zhou F, Gan Y, Zhang YH, Ma L Tags: J Zhejiang Univ Sci B Source Type: research

Toxicity and metabolism of 3-bromopyruvate in Caenorhabditis elegans.
In this study, we aimed to evaluate the toxic effects, changes in life span, and expression of various metabolism-related genes in Caenorhabditis elegans, using RNA interference (RNAi) and mutant strains, after 3-bromopyruvate (3-BrPA) treatment. C. elegans was treated with various concentrations of 3-BrPA on nematode growth medium (NGM) plates, and their survival was monitored every 24 h. The expression of genes related to metabolism was measured by the real-time fluorescent quantitative polymerase chain reaction (qPCR). Nematode survival in the presence of 3-BrPA was also studied after silencing three hexokinase (HK) gen...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Gu QL, Zhang Y, Fu XM, Lu ZL, Yu Y, Chen G, Ma R, Kou W, Lan YM Tags: J Zhejiang Univ Sci B Source Type: research

Modified stepwise mini-incision microdissection testicular sperm extraction: a useful technique for patients with a history of orchidopexy affected by non-obstructive azoospermia.
Abstract Non-obstructive azoospermia (NOA), which is defined as the absence of spermatozoa in the ejaculate secondary to impaired spermatogenesis within the testis, may be caused by a variety of etiologies, including varicocele-induced testicular damage, cryptorchidism, prior testicular torsion, post-pubertal mumps orchitis, gonadotoxic effects from medications, genetic abnormalities, chemotherapy/radiation, and other unknown causes currently classified as idiopathic (Cocuzza et al., 2013). The microdissection testicular sperm extraction (micro-TESE) technique involves a meticulous microsurgical exploration of the...
Source: J Zhejiang Univ Sci ... - January 1, 2020 Category: Science Authors: Li P, Yao CC, Zhi EL, Xu Y, Wan Z, Jiang YC, Huang YH, Gong YH, Chen HX, Tian RH, Yang C, Zhao LY, Li Z Tags: J Zhejiang Univ Sci B Source Type: research

Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes.
CONCLUSIONS: This study demonstrated the feasibility of customized design, analysis, and fabrication of a corneal substitute. The programmability of this method opens up the possibility of producing substitutes for other cornea-like shell structures with different scale and geometry features, such as the glomerulus, atrium, and oophoron. PMID: 31749342 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Zhang B, Xue Q, Hu HY, Yu MF, Gao L, Luo YC, Li Y, Li JT, Ma L, Yao YF, Yang HY Tags: J Zhejiang Univ Sci B Source Type: research

All-trans-retinoic acid generation is an antidotal clearance pathway for all-trans-retinal in the retina.
Abstract The present study was designed to analyze the metabolites of all-trans-retinal (atRal) and compare the cytotoxicity of atRal versus its derivative all-trans-retinoic acid (atRA) in human retinal pigment epithelial (RPE) cells. We confirmed that atRA was produced in normal pig neural retina and RPE. The amount of all-trans-retinol (atROL) converted from atRal was about 2.7 times that of atRal-derived atRA after incubating RPE cells with 10 μmol/L atRal for 24 h, whereas atRA in medium supernatant is more plentiful (91 vs. 29 pmol/mL), suggesting that atRA conversion facilitates elimination of excess atR...
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Xia QQ, Zhang LM, Zhou YY, Wu YL, Li J Tags: J Zhejiang Univ Sci B Source Type: research

Effects of calcium-binding sites in the S2-S3 loop on human and Nematostella vectensis TRPM2 channel gating processes.
Abstract As a crucial signaling molecule, calcium plays a critical role in many physiological and pathological processes by regulating ion channel activity. Recently, one study resolved the structure of the transient receptor potential melastatin 2 (TRPM2) channel from Nematostella vectensis (nvTRPM2). This identified a calcium-binding site in the S2-S3 loop, while its effect on channel gating remains unclear. Here, we investigated the role of this calcium-binding site in both nvTRPM2 and human TRPM2 (hTRPM2) by mutagenesis and patch-clamp recording. Unlike hTRPM2, nvTRPM2 cannot be activated by calcium alone. Mor...
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Luo YH, Yu XF, Ma C, Yang F, Yang W Tags: J Zhejiang Univ Sci B Source Type: research

Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB.
Abstract Genome sequencing projects revealed massive cryptic gene clusters encoding the undiscovered secondary metabolites in Streptomyces. To investigate the metabolic products of silent gene clusters in Streptomyces chattanoogensis L10 (CGMCC 2644), we used site-directed mutagenesis to generate ten mutants with point mutations in the highly conserved region of rpsL (encoding the ribosomal protein S12) or rpoB (encoding the RNA polymerase β-subunit). Among them, L10/RpoB (H437Y) accumulated a dark pigment on a yeast extract-malt extract-glucose (YMG) plate. This was absent in the wild type. After further inv...
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Li ZY, Bu QT, Wang J, Liu Y, Chen XA, Mao XM, Li YQ Tags: J Zhejiang Univ Sci B Source Type: research

Cloning and characterization of an oxiranedicarboxylate hydrolase from Labrys sp. WH-1.
CONCLUSIONS: The catalytic efficiency and thermal stability of the ORCH from Labrys sp. WH-1 were the best among the reported ORCHs, and it provides an alternative catalyst for preparation of L(+)-2,3-dihydrobutanedioic acid. PMID: 31749346 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Bao WN, Luo ZS, Liu SW, Wu YF, Wei PL, Xiao GN, Liu Y Tags: J Zhejiang Univ Sci B Source Type: research

A novel oncolytic adenovirus inhibits hepatocellular carcinoma growth.
CONCLUSIONS: GP73-SphK1sR-Ad5 serves as a novel OA and can inhibit HCC progression with high specificity and efficacy. PMID: 31749347 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Bai YH, Yun XJ, Xue Y, Zhou T, Sun X, Gao YJ Tags: J Zhejiang Univ Sci B Source Type: research

Detection and segmentation of multi-class artifacts in endoscopy.
Abstract Endoscopy may be used for early screening of various cancers, such as nasopharyngeal cancer, esophageal adenocarcinoma, gastric cancer, colorectal cancer, and bladder cancer, and performing minimal invasive surgical procedures, such as laparoscopy surgery. During this procedure, an endoscope is used; it is a long, thin, rigid, or flexible tube having a light source and a camera at the tip, which facilitates visualization inside the affected organs on a screen and helps doctors in diagnosis. PMID: 31749348 [PubMed - in process] (Source: J Zhejiang Univ Sci ...)
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Zhang YY, Xie D Tags: J Zhejiang Univ Sci B Source Type: research

Mycoplasma contamination-mediated attenuation of plasmid DNA transfection efficiency is augmented via L-arginine deprivation in HEK-293 cells.
Abstract Mycoplasma infection is the most prevalent contamination in cell culture. Analysis of cell culture in laboratories from different countries shows that mycoplasma contamination ranges from 15% to 80% and, in some cases, even reaches 100% (Chernov et al., 2014). Whilst mycoplasma infection is not visible to the naked eye in cell culture, the consequences of mycoplasma contamination have been shown to induce a number of cellular changes, for example, increased resistance to chemotherapeutic drugs. Therefore, any results obtained from tissue culture studies, in the presence of mycoplasma contamination, potent...
Source: J Zhejiang Univ Sci ... - November 23, 2019 Category: Science Authors: Yin ZF, Zhang YN, Liang SF, Zhao SS, Du J, Cheng BB Tags: J Zhejiang Univ Sci B Source Type: research

Automated microfluidic chip system for radiosynthesis of PET imaging probes.
Abstract Positron emission tomography (PET) is a powerful non-invasive molecular imaging technique for the early detection, characterization, and "real-time" monitoring of disease, and for investigating the efficacy of drugs (Phelps, 2000; Ametamey et al., 2008). The development of molecular probes bearing short-lived positron-emitting radionuclides, such as 18F (half-life 110 min) or 11C (half-life 20 min), is crucial for PET imaging to collect in vivo metabolic information in a time-efficient manner (Deng et al., 2019). In this regard, one of the main challenges is rapid synthesis of radiolabeled probe...
Source: J Zhejiang Univ Sci ... - October 10, 2019 Category: Science Authors: Lei M, Pan JZ, Xu GM, Du PZ, Tian M, Zhang H Tags: J Zhejiang Univ Sci B Source Type: research