Cancer signaling pathways with a therapeutic approach: An overview in epigenetic regulations of cancer stem cells
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Ali Akbar Samadani, Syedeh Elham Norollahi, Ali Rashidy-Pour, Fariborz Mansour-Ghanaei, Shadman Nemati, Farahnaz Joukar, Amir Mahmoud Afshar, Sahar Ghazanfari, Mahsa Safizadeh, Pooya Rostami, Magtouf GateiAbstractOne of the most important issues in cancer progression is caner stem cells (CSCs) which have illustrated that the bulk tumors can arise from a special combination of cells. Remarkably, it has been proposed to be a notable and strong factor in carcinogenesis and tumorogenesis and also is a key parameter of therapeutic res...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Dual gene deficient models of ApcMin/+ mouse in assessing molecular mechanisms of intestinal carcinogenesis
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Shuwen Yu, Yanhui Yin, Qian Wang, Lu WangAbstractThe ApcMin/+ mouse, carrying an inactivated allele of the adenomatous polyposis coli (Apc) gene, is a widely used animal model of human colorectal tumorigenesis. While crossed with other gene knockout or knock-in mice, these mice possess advantages in investigation of human intestinal tumorigenesis. Intestinal tumor pathogenesis involves multiple gene alterations; thus, various double gene deficiency models could provide novel insights into molecular mechanisms of tumor biology, as...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Molecular pathways associated with oxidative stress in diabetes mellitus
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Osasenaga Macdonald IghodaroAbstractThe role of oxidative stress in the occurrence and development of diabetes mellitus is both critical and pivotal. Several molecular event cascade in different metabolic pathways such as glycolytic, hexosamine, protein kinase C, polyol and advanced glycation end-product (AGE) pathways have been identified as pro-oxidative processes and are usually up-regulated in the diabetics. Inhibition of glyceraldehyde-3-P dehydrogenase by poly-ADP-ribose polymerase 1 and subsequent accumulation of the enzym...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

miR-326 functions as a tumor suppressor in human prostatic carcinoma by targeting Mucin1
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Xuan Liang, Zhaolun Li, Qunli Men, Yongwei Li, Hechen Li, Tie ChongAbstractAccumulating evidence suggests that microRNA-326 (miR-326) serves as a tumor suppressor in the initiation and progression of several human malignancies. However, the biological function and underlying molecular mechanism of miR-326 in prostatic carcinoma (PCa) remains largely unknown. In the present study, we found that miR-326 expression level was significantly downregulated in both primary PCa and castration-resistant PCa (CRPC) tissue samples as detecte...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Silencing Bmi1 expression suppresses cancer stemness and enhances chemosensitivity in endometrial cancer cells
ConclusionBmi1 silencing suppresses cancer stemness in HEC1A and Ishikawa cells. Concurrent treatment with Bmi1 siRNA and cisplatin resulted in additive anticancer effect with a cell line-specific pattern, which was higher than that shown by cisplatin treatment alone.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Generation of a novel long-acting thymosin alpha1-Fc fusion protein and its efficacy for the inhibition of breast cancer in vivo
In this study, we fused Tα1 to Fc domain of human IgG1 for generation of a novel long-acting fusion protein, termed Tα1-Fc. Tα1-Fc was expressed, purified, and identified. The results showed that Tα1-Fc was more potent than Tα1 in inhibiting the growth of 4T1 and MCF-7 breast cancer cells in vivo. Furthermore, in the 4T1 tumor model the mice treated with Tα1-Fc exhibited higher level of CD4 and CD8 cells compared with that of the mice Tα1 treated. The interferon-γ and interleukin-2 level in the Tα1-Fc-treated mice was higher than that in the Tα1-treated mice. T&al...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

PMA treated THP-1-derived-IL-6 promotes EMT of SW48 through STAT3/ERK-dependent activation of Wnt/β-catenin signaling pathway
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Sikang Gao, Junwu Hu, Xiongwen Wu, Zhihui LiangAbstractColon cancer is one of the most common digestive malignant tumors that leads to high mortality worldwide, and metastasis is the primary cause of cancer-related death. It is well accepted that the epithelial-mesenchymal transition (EMT) plays a key role in the process of metastasis. As a cytokine that macrophage secretes, IL-6 is involved in the progression of tumors, including the invasion and metastasis via kinds of signaling pathways. However, the mechanism of interactions ...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Protective effect of syringaldehyde on biomolecular oxidation, inflammation and histopathological alterations in isoproterenol induced cardiotoxicity in rats
ConclusionSYD had cardioprotective efficacy owing to its antioxidative and anti-inflammatory properties. Our results support incorporation of SYD in regular diet for prevention of MI.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Protective effect of wax apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) against streptozotocin-induced pancreatic ß-cell damage in diabetic rats
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Aree Khamchan, Tatdao Paseephol, Wanthanee HanchangAbstractThe purpose of this study was to investigate the protective properties and mechanisms of wax apple (Syzygium samarangense (Blume)) against streptozotocin (STZ)-induced pancreatic ß-cell apoptosis in diabetic rats. Diabetes was induced by STZ (65 mg/kg; i.p.) injection and wax apple (100 mg/kg) was orally administered to diabetic rats for a period of 30 days. During this time, fasting blood glucose (FBG) and body weight were measured weekly. At the end ...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Transcription factor 7-like 2 controls matrix degradation through nuclear factor κB signaling and is repressed by microRNA-155 in nucleus pulposus cells
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Jianchao Sun, Junmin Hong, Siguo Sun, Xiaofei Wang, Yan Peng, Jie Zhou, Yingjie Huang, Shuangxing Li, Weijian Chen, Chunhai Li, Kang Xu, Wei YeAbstractAimTCF7L2, a key transcription factor in the canonical Wnt pathway, plays a vital role in the matrix degradation of chondrocytes. However, it is unknown whether TCF7L2 is important in the matrix metabolism of inner gel-like nucleus pulposus (NP) cells; thus, the aim of this study was to explore the effect and mechanism of TCF7L2 in this process.MethodsWestern blotting and immunoflu...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Icariin and mesenchymal stem cells synergistically promote angiogenesis and neurogenesis after cerebral ischemia via PI3K and ERK1/2 pathways
In this study, male SD rats were subjected to transient middle cerebral artery occlusion (MCAO) followed by reperfusion and ICA/MSC treatment. Results showed that ICA and MSCs combined treatment greatly reduced brain infarction volume, improved neurologic deficits of motor and somatosensory function and neurobehavioral outcomes. The combined therapy increased expression of VEGF and BDNF to a maximum through activating PI3K and ERK1/2 pathways in the hippocampus and frontal cortex in response to transient MCAO. They notably promoted angiogenesis and neurogenesis in vivo. Thus, ICA and MSCs combined treatment may represent a...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Design, synthesis, in vitro, in vivo and in silico pharmacological characterization of antidiabetic N-Boc-l-tyrosine-based compounds
In conclusion, we demonstrated that both molecules showed strong in vitro and in vivo effects and can be considered polypharmacological antidiabetic candidates.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Astragalosides IV protected the renal tubular epithelial cells from free fatty acids-induced injury by reducing oxidative stress and apoptosis
This study may provide a new theoretical option for the patients with type 2 diabetes. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Melatonin improves vascular cognitive impairment induced by ischemic stroke by remyelination via activation of ERK1/2 signaling and restoration of glutamatergic synapses in the gerbil hippocampus
In this study, effects of melatonin on cognitive impairment and related mechanisms were investigated in an animal model of ischemic vascular dementia (IVD). Melatonin was intraperitoneally administered to adult gerbils after transient global cerebral ischemia (tGCI) for 25 days beginning 5 days after tGCI. Cognitive impairment was examined using a passive avoidance test and the Barnes maze test. To investigate mechanisms of restorative effects by melatonin, neuronal damage/death, myelin basic protein (MBP, a marker for myelin), Rip (a marker for oligodendrocyte), extracellular signal-regulated protein kinase1/2 (ERK1/2) an...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Syringic acid (SA) ‒ A Review of Its Occurrence, Biosynthesis, Pharmacological and Industrial Importance
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Cheemanapalli Srinivasulu, Mopuri Ramgopal, Golla Ramanjaneyulu, C.M. Anuradha, Chitta Suresh KumarAbstractThe use of phytochemicals in control of human diseases have been considerable public and scientific interest in current days. Syringic acid (SA), a phenolic compound often found in fruits and vegetables and which is synthesized via shikimic acid pathway in plants. It shows a wide range of therapeutic applications in prevention of diabetes, CVDs, cancer, cerebral ischemia; as well as it possess anti-oxidant, antimicrobial, an...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Equine suspensory ligament and tendon explants cultured with platelet-rich gel supernatants release different anti-inflammatory and anabolic mediators
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Andrés F. Bonilla-Gutiérrez, Cristian Castillo-Franz, Catalina López, María E. Álvarez, Carlos E. Giraldo, Jorge U. CarmonaAbstractThe aim of this study was to evaluate the release of pro- and anti-inflammatory as well as anabolic mediators stimulated by a leukocyte-reduced platelet-rich gel supernatant (Lr-PRGS) and a leukocyte-reduced plasma supernatant (Lr-PL) at two concentrations (25 and 50%) on normal equine suspensory ligament explants (SLEs) and tendon explants (TEs). SLEs and TEs from s...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Lobaplatin suppresses proliferation and peritoneal metastasis of colorectal cancer in a preclinical model
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Lina Shan, Bingjun Bai, Yiming Lv, Binbin Xie, Xuefeng Huang, Hongbo ZhuAbstractPeritoneal metastasis from colorectal cancer (CRC) is related to poor prognosis. Intraperitoneal chemotherapy is an efficient method to treat peritoneal metastasis (PM); however, the outcomes remain unsatisfactory. The present study aimed to investigate the antitumor activity of lobaplatin and its role in intraperitoneal chemotherapy. The findings showed that the proliferation of CRC was inhibited in a dose-dependent manner when DLD1 and HCT116 cells ...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

PNMA1, regulated by miR-33a-5p, promotes proliferation and EMT in hepatocellular carcinoma by activating the Wnt/β-catenin pathway
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Pengpeng Liu, Baiyan Chen, Yang Gu, Quanyan LiuAbstractHepatocellular carcinoma (HCC) is one of the most common malignant tumors throughout the world. However, its mortality rate remains very high due to the absence of early diagnostic modalities and effective treatments, and its specific pathogenesis remains unclear. Here, we investigated the role of PNMA1 in the tumorigenesis of HCC. We found that PNMA1 was significantly upregulated in HCC. Clinically, higher expression of PNMA1 was associated with aggressive phenotypes and poo...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Identification of oncogenic long noncoding RNA SNHG12 and DUXAP8 in human bladder cancer through a comprehensive profiling analysis
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Bin Jiang, Su Hailong, Jun Yuan, Hu Zhao, Wenkai Xia, Zhenlei Zha, Wu Bin, Zhili LiuAbstractBladder cancer is a common urological malignancies world-wide. Recently, a growing number of evidence have highlighted the importance of long noncoding RNAs (lncRNAs) in multiple cancers development and progression. However, the expression pattern of lncRNAs in bladder cancer and their underlying function remain poorly understood. To identify lncRNAs profile alterations and uncover valuable lncRNA candidates for bladder cancer diagnostic, ...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Co-expression of Akt1 and Wnt11 promotes the proliferation and cardiac differentiation of mesenchymal stem cells and attenuates hypoxia/reoxygenation-induced cardiomyocyte apoptosis
In this study, we genetically engineered MSCs using ex vivo adeno-associated virus (AAV) transduction to overexpress Akt1 and Wnt11, which are well-characterized genes involved in MSC proliferation and cardiac differentiation. Our results showed that infection with AAV-Akt1-Wnt11 significantly upregulated the growth and proliferation of MSCs, as compared with those infected with AAV-Akt1 or AAV-Wnt11. In addition, co-expression of Akt1 and Wnt11 markedly promoted the expression of cardiac markers including NK2 transcription factor related 5, GATA-binding protein 4, α-myosin MHC and brain natriuretic protein. Notably,...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Evaluating the protective effects of melatonin on di(2-ethylhexyl) phthalate-induced testicular injury in adult mice
ConclusionMelatonin considerably inhibits DEHP-induced gonadotoxicity through reducing oxidative stress and inflammatory responses. These results suggest that melatonin may be considered as a promising agent to reduce toxic effects of endocrine disrupting chemicals such as DEHP on the male reproductive system. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Usnic acid inhibits hypertrophic scarring in a rabbit ear model by suppressing scar tissue angiogenesis
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Yajuan Song, Zhou Yu, Baoqiang Song, Shuzhong Guo, Lei Lei, Xianjie Ma, Yingjun SuAbstractHypertrophic scarring is a common condition in the Chinese population; however, there are currently no satisfactory drugs to treat the disorder. Previous studies showed that angiogenesis plays an important role in the early phase of hypertrophic scarring and inhibition of angiogenesis has been reported as an effective strategy for anti-hypertrophic scar therapy. A recent study showed that usnic acid (UA), an active compound found mainly in l...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

MicroRNA-143a-3p modulates preadipocyte proliferation and differentiation by targeting MAPK7
In this study, miR-143a-3p was highly expressed in adipose tissues of obese mice, and was up-regulated at the middle and last stage of 3T3-L1 adipocyte differentiation. Using mouse 3T3-L1 cells line, which is an ideal model in vitro for the study of adipogenesis, we observed that overexpression of miR-143a-3p inhibited the preadipocyte proliferation, and enhanced the preadipocyte differentiation. In contrast, the inhibition of miR-143a-3p expression promoted the preadipocyte proliferation, and inhibited the preadipocyte differentiation. Further analysis suggested that miR-143a-3p mediating preadipocyte differentiation migh...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

The effect of serine phosphorylated claudin-7 on the epithelial barrier and the modulation by transient receptor potential vanilloid 4 in human colonic cells
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Yuan-Yuan Huang, Zhen-Kai Wang, Jing Li, Su-Wen Bai, Bing Shen, Juan Du, Xian-Ming Xia, Fang-Yu WangAbstractAbnormal phosphorylation of claudins changes the interaction and aggregation of tight junction proteins, affecting the intestinal epithelial barrier. Selective blockade of transient receptor potential vanilloid 4 (TRPV4) alleviated experimental colitis. Whether TRPV4 affects the intestinal epithelial barrier and the relationship to claudin-7 phosphorylation remain unknown. In the present study, we investigated the TRPV4 exp...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

In vivo antifungal activity of dipyrithione against Trichophyton rubrum on guinea pig dermatophytosis models
In this study, we determined that dipyrithione could potently inhibit the growth of Trichophyton rubrum, which is the most common cause of dermatophytosis. The MIC50 value of dipyrithione against T. rubrum was measured as 6.03 μM, as compared with miconazole (MIC50: 1.38 μM). Additionally, the compound caused morphological changes in the fungi, which was examined using the morphological interference assay. The in vivo experiment further revealed that dipyrithione had a healing effect on the skin of guinea pigs infected with T. rubrum. Our studies have demonstrated that dipyrithione had a potent antifung...
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Encapsulation of verapamil and doxorubicin by MPEG-PLA to reverse drug resistance in ovarian cancer
ConclusionCo-delivery of verapamil and chemotherapeutic drugs by MPEG-PLA efficiently reversed the drug resistance, resulting in enhanced anticancer effects along with reduced systemic toxicity, which provides potential clinical applications for drug resistant ovarian cancer treatment.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

The silencing of ApoC3 suppresses oxidative stress and inflammatory responses in placenta cells from mice with preeclampsia via inhibition of the NF-κB signaling pathway
ConclusionSilencing ApoC3 could suppress the NF-κB signaling pathway, thereby exercising a protective effect on cell injury induced by oxidative stress and reducing inflammatory responses.Graphical abstractThe molecular mechanism involving silenced APOC3 regulating the oxidative stress and inflammatory responses in placenta cells of preeclampsia mice via inhibition of the NF-κB signaling pathway. In preeclampsia mice, up-regulated APOC3 increased the expression of p65 and inhibited the expression of phosphorylation of IkBα, thus resulting in the oxidative stress injury and inflammatory responses of placen...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

JS-K as a nitric oxide donor induces apoptosis via the ROS/Ca2+/caspase-mediated mitochondrial pathway in HepG2 cells
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Zile Huang, Ling Liu, Jingjing Chen, Mengyao Cao, Jiangang WangAbstractJS-K, (O2-(2, 4-dinitrophenyl) 1-[(4-ethoxycarbonyl) piperazin-1-yl] diazen 1-ium-1, 2-diolate), is a novel diazeniumdiolate-based nitric oxide donor prodrug. The present study investigated the relationship between reactive oxygen species (ROS) elevation, Ca2+ overload and mitochondrial disruption in JS-K-induced apoptosis. JS-K could significantly inhibit cell growth and induce apoptosis in a dose-dependent manner. Meanwhile, JS-K caused the accumulation of R...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Knockdown of TLR4 attenuates high glucose-induced podocyte injury via the NALP3/ASC/Caspase-1 signaling pathway
In conclusion, knockdown of TLR4 attenuates HG-induced podocyte injury via the NALP3/ASC/Caspase-1 signaling pathway. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Monitoring cellular immune function of renal transplant recipients based on adenosine triphosphate (ATP) production by mitogen-induced CD4+ T helper cells
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Hadi Naderi, Gholamreza Pourmand, Sanaz Dehghani, Hassan Nikoueinejad, Mohammad Jafari, Nader TajikAbstractMaintaining the balance between over- and under-immunosuppression has a critical role for successful immunosuppressive therapy after renal transplantation. We studied the predictive value of our functional immune assay, which works based on adenosine triphosphate (ATP) levels, in determining risk of infection and rejection among renal transplant recipients (RTRs). A total of 65 RTRs with less than 1 month (RTRL1) and 48 RTRs...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

miR-378a-3p sensitizes ovarian cancer cells to cisplatin through targeting MAPK1/GRB2
In conclusion, miR-378a-3p enhanced the sensitivity of ovarian cancer cells to cisplatin through targeting MAPK1 and GRB2.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Ozone therapy ameliorates inflammation and endometrial injury in rats with pelvic inflammatory disease
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): An Wei, Hao Feng, Xiao-Min Jia, Hua Tang, Yang-Ying Liao, Bi-Rong LiAbstractAs a common cause of infertility, pelvic inflammatory disease (PID) is characterized by chronic pain, ectopic pregnancy as well as inflammation and infection of the female upper genital tract. Ozone water, also known as O3, has been previously reported to be a distinctly effective agent in treating inflammation. During the present study, we asserted the hypothesis that O3 could be applied by pelvic inflammation and works to regulate the expression of infl...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Daphnegiravone D from Daphne giraldii Nitsche induces p38-dependent apoptosis via oxidative and nitrosative stress in hepatocellular carcinoma cells
In conclusion, the induction of oxidative and nitrosative stress could enhance p38-mediated apoptosis in DGD-treated Hep3B and HepG2 cells. Moreover, we speculated that OS and NS could not ultimately affect each other in DGD-treated HCC cells. This study gives a new insight on the mechanism of DGD-induced apoptotic cell death via oxidative and nitrosative stress in HCC cells.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

SNHG6 functions as a competing endogenous RNA to regulate E2F7 expression by sponging miR-26a-5p in lung adenocarcinoma
In this study, SNHG6 was upregulated in LUAD tissues, and its upregulation was positively associated with advanced TNM stage, large tumour size and poor overall survival (OS) in LUAD patients. Gain- and loss-of-function experiments confirmed that SNHG6 promoted cell cycle progression, cell proliferation, migration and invasion, and epithelial-mesenchymal transition (EMT) in vitro. Animal experiments demonstrated that SNHG6 knockdown remarkably inhibited xenograft formation in vivo. Moreover, mechanistic experiments identified that SNHG6 functions as a competing endogenous RNA (ceRNA) through competitively sponging miR-26a-...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Effects of berberine on tumor growth and intestinal permeability in HCT116 tumor-bearing mice using polyamines as targets
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Yan-Yan Wu, Tong-Ming Li, Lin-Quan Zang, Bing Liu, Gui-Xiang WangAbstractThe prognosis of colorectal cancer (CRC) is seriously affected by high intestinal mucosal permeability accompanied by increasing tumor load. Berberine, a natural plant-derived product, can protect the intestinal mucosal barrier and suppress tumor growth, but its effects on the intestinal mucosal barrier dysfunction of CRC have not yet been evaluated. Herein, we assessed the effects of berberine on the intestinal mucosal permeability of HCT116 tumor-bearing m...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

TREM1: A positive regulator for inflammatory response via NF-κB pathway in A549 cells infected with Mycoplasma pneumoniae
In this study, A549 cells, known as human lung epithelial cells, were co-cultured with 107 CCU/ml of MP to established in vitro model of MP infection. We studied the roles of TREM1 in inflammatory response of A549 cell by regulating the secretions of cytokine interleukin (IL)-8 and tumor necrosis factor (TNF)-α in cell culture supernatants. Moreover, transcriptional activity of nuclear factor kappa B (NF-кB) was assessed by measuring protein levels of NF-кB in the cytoplasm and nuclear. Our data suggested that sanguinarine chloride significantly decreased TREM1 expression, and alleviated inflammatory response of MP...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Modulatory effect of quercetin and its glycosylated form on key enzymes and antioxidant status in rats penile tissue of paroxetine-induced erectile dysfunction
This study sought to compare the effects of quercetin and rutin on some enzymes linked to erectile function as well as antioxidant status in penile tissue of paroxetine – induced erectile dysfunction in rats. Animals were randomly divided into twelve groups: normal control (NC), sildenafil (SD), quercetin (QA) (25 and 50 mg/kg), rutin (RU) (25 and 50 mg/kg), PAR (10 mg/kg); PAR + SD; PAR + QA, PAR + RU (25 and 50 mg/kg). After 14 days’ treatment, phosphodiesterase-5′ (PDE-5′), arginase, adenosine deaminase (ADA), acetylcholinester...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Mulberrin (Mul) reduces spinal cord injury (SCI)-induced apoptosis, inflammation and oxidative stress in rats via miroRNA-337 by targeting Nrf-2
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Peng Xia, Xu Gao, Lian Duan, Wei Zhang, Yi-Fu SunAbstractSpinal cord injury (SCI) is a major reason of paralysis, disability and even death in severe cases. Mulberrin (Mul) is a major compound of ramulus mori and turned out that it has potent ability on tyrosinase inhibition. Ramulus mori has been reported to possess anti-inflammation, anti-oxidative stress and anti-apoptosis effects. However, the effects of Mul on SCI progression and the underlying molecular mechanism have never been elucidated before. Here, we established a SCI...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Parthenolide inhibits tumor-promoting effects of nicotine in lung cancer by inducing P53 - dependent apoptosis and inhibiting VEGF expression
In this study, we tested the ability of parthenolide to inhibit the proliferating effect of nicotine in lung cancer cell lines. MTT assay was used to measure cell survival of A549 and H526 cells treated with nicotine, parthenolide, and their combination. Angiogenesis inhibition was measured using VEGF detection kit and apoptosis induction was evaluated by measuring caspase-3 activity. Real time PCR assay was used to detect the change in expression of several genes associated with cell proliferation and apoptosis (CASP3, CASP7, CASP8, CASP9, P53, GADD45, BAX, BIM, Bcl-2, TOPO I, and TOPO II). Parthenolide inhibited lung can...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

CXCL5, the upregulated chemokine in patients with uterine cervix cancer, in vivo and in vitro contributes to oncogenic potential of Hela uterine cervix cancer cells
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Xiaona Feng, Danfeng Zhang, Xinyi Li, Shuxia Ma, Chunbin Zhang, Jingtao Wang, Yue Li, Lichun Liang, Pengxia Zhang, Yikun Qu, Zeyu Zhang, Zhe Yang, Yu Xiang, Weili Zhang, Shuqiu Wang, Wenwu Shao, Weiqun WangAbstractCXCL5 is showed a surprisingly elevated profile and implicated in tumorigenesis in several tumors. However, the expression and function of CXCL5 in uterine cervix cancer (UCC) remain largely unknown. The current study aimed to elucidate the expression pattern of CXCL5 in human UCC tissues and Hela cervix cancer cell, as...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Scutellarin inhibits human renal cancer cell proliferation and migration via upregulation of PTEN
ConclusionScutellarin inhibited RCC cell proliferation and invasion partially by enhancing the expression of PTEN through inhibition of P13K/AKT/mTOR pathway, suggesting that Scutellarin might serve as a potential therapeutic agent in RCC treatment.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

6-Gingerol attenuates LPS-induced neuroinflammation and cognitive impairment partially via suppressing astrocyte overactivation
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Feng Zhang, Ji-Guo Zhang, Wei Yang, Pu Xu, Yu-Liang Xiao, Han-Ting ZhangAbstract6-Gingerol, the major component of gingerols extracted from Zingiber officinale, has been shown to exhibit anti-inflammatory and antioxidant bioactivities. Since neuroinflammation plays an important role in neurodegenerative diseases, such as Alzheimer’s disease (AD), and astrocytes have been considered important in the process of neurodegeneration, it was of interest to know whether 6-gingerol reduced astrocytes activation or even attenuated co...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Monocyte subpopulations as important biomarkers of resistence and susceptibility during experimental infection with Leishmania (Leishmania) major
In this study, we have analyzed the frequency of monocyte subpopulations in mice infected with Leishmania major (L. major). Our results show a significant correlation between increased blood monocyte frequency and lesion development in both BALB/c and in the C57BL/6 mice infected with L. major. In BALB/c mice we observed a significant correlation between the frequency of GR1+ monocytes and lesion size. Furthermore, treatment of infected BALB/c mice with Anfotericin B, to resolve lesions, resulted in a lower frequency of GR1+ monocytes compared to untreated infected BALB/c mice. C57BL/6 infected mice, which normally resolve...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

LncRNA PRNCR1 interacts with HEY2 to abolish miR-448-mediated growth inhibition in non-small cell lung cancer
In this study, miR-448 was expressed higher in NSCLC tissues (P 
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

DIXDC1 promotes the growth of acute myeloid leukemia cells by upregulating the Wnt/β-catenin signaling pathway
In this study, we aimed to investigate the expression status and potential biological function of DIXDC1 in AML. Our results showed that DIXDC1 expression was highly upregulated in AML cell lines and primary AML blasts compared with normal blasts. Knockdown of DIXDC1 by siRNA-mediated gene silencing significantly inhibited proliferation, induced cell cycle arrest, and promoted apoptosis of AML cells in vitro. By contrast, DIXDC1 overexpression promoted proliferation, accelerated cell cycle progression, and reduced apoptosis of AML cells. Moreover, we found that DIXDC1 knockdown decreased the expression of β-catenin an...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Berberine treatment reduces atherosclerosis by mediating gut microbiota in apoE-/- mice
ConclusionThe antiatherosclerotic effect of BBR is related to alterations in gut microbiota compositions, indicating the potential therapeutic value of pharmacological approaches that may modulate the gut microbiota in treating atherosclerosis. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Baicalein inhibits proliferation and migration of bladder cancer cell line T24 by down-regulation of microRNA-106
ConclusionBAI suppressed proliferation and migration, and induced apoptosis of T24 cells through down-regulating miR-106, along with inhibition of the JNK and MEK/ERK pathways. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

IL-1β reduces cardiac lymphatic muscle contraction via COX-2 and PGE2 induction: Potential role in myocarditis
Publication date: November 2018Source: Biomedicine & Pharmacotherapy, Volume 107Author(s): Mahmoud Al-Kofahi, Seiichi Omura, Ikuo Tsunoda, Fumitaka Sato, Felix Becker, Felicity N.E. Gavins, Matthew D. Woolard, Christopher Pattillo, David Zawieja, Mariappan Muthuchamy, Anatoliy Gashev, Israa Shihab, Mohamed Ghoweba, Pierre-Yves Von der Weid, Yuping Wang, J. Steven AlexanderAbstractThe role of lymphatic vessels in myocarditis is largely unknown, while it has been shown to play a key role in other inflammatory diseases. We aimed to investigate the role of lymphatic vessels in myocarditis using in vivo model induced with T...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Brachychiton populneus (Schott & Endl.) R.Br. ameliorate carbon tetrachloride induced oxidative stress through regulation of endoplasmic reticulum stress markers and inflammatory mediators in Sprague-Dawley male rats
In this study hepatoprotective aptitude of Brachychiton populneus against carbon tetrachloride (CCl4) instigated liver injuries in rats was investigated. High-performance liquid chromatography (HPLC) with a diode array detector (DAD) analysis of methanol extract of B. populneus (BPM) indicated existence of rutin, catechin and myricetin. Administration of CCl4 to rat decreased (p 
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Scaffold hopping-guided design of some isatin based rigid analogs as fatty acid amide hydrolase inhibitors: Synthesis and evaluation
In conclusion, the present study provided a better understanding of the molecular fragments requisite for maintaining and/or improving FAAH inhibition activity.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research