RNA-based pharmacotherapy for tumors: From bench to clinic and back
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Xiangping Liang, Dongpei Li, Shuilong Leng, Xiao ZhuAbstractRNA therapy is a treatment that regulates cell proteins and cures diseases by affecting the metabolism of mRNAs in cells, which has cut a figure in the studies on various incurable illnesses like hereditary diseases, tumors, etc. In this review, we introduced the discovery and development of RNA therapy and discussed its classification, mechanisms, advantages, and challenges. Moreover, we highlighted how RNA therapy works in killing tumor cells as well as what progresses it h...
Source: Biomedicine and Pharmacotherapy - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Long noncoding RNA TMPO-AS1 promotes lung adenocarcinoma progression and is negatively regulated by miR-383-5p
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Xiaoqian Mu, Hongbo Wu, Jie Liu, Xiufeng Hu, Huijuan Wu, Lijuan Chen, Wenjing Liu, Suxia Luo, Yanqiu ZhaoAbstractLong noncoding RNAs (lncRNAs) play critical roles in the pathogenesis of various diseases, including a variety of tumors. Nevertheless, its functional roles and underlying molecular basis for their dysregulation in lung adenocarcinoma (LUAD) are largely unknown. Herein, in our study, we identified that lncRNA TMPO-AS1 is significantly upregulated in LUAD tissues and cell lines. Knockdown of TMPO-AS1 remarkably suppressed LU...
Source: Biomedicine and Pharmacotherapy - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

LncRNA HOTAIR knockdown inhibits glycolysis by regulating miR-130a-3p/HIF1A in hepatocellular carcinoma under hypoxia
In conclusion, knockdown of HOTAIR suppressed glycolysis by regulating miR-130a-3p and HIF1A in HCC cells treated by hypoxia, elucidating a novel mechanism in HCC glycolysis. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 15, 2020 Category: Drugs & Pharmacology Source Type: research

Pursuing for the better lung cancer therapy effect: Comparison of two different kinds of hyaluronic acid and nitroimidazole co-decorated nanomedicines
In this study, we attempted to fabricate Nitroimidazoles (NI) and Hyaluronic acid (HA) co-decorated, cisplatin (DDP) loaded polymeric nanoparticles (PNPs) (NI/HA-DDP-PNPs) and lipid-polymer hybrid nanoparticles (LPNs) (NI/HA-DDP-LPNs) for the facilitated drug delivery at lung tumor regions (hypoxic regions). In vitro cytotoxicity and cellular uptake; In vivo anti-tumor activity and in vivo tissue biodistribution of PNPs and LPNs were evaluated and compared in lung carcinoma cells and xenograft. Hydrodynamic size of NI/HA-DDP-LPNs was 185.6 ± 4.7 nm, which is larger than that of NI/HA-DDP-PNPs (136.7 ± 3.5 nm)...
Source: Biomedicine and Pharmacotherapy - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis
ConclusionOur data provided strong evidence for the participation of MIF in the pathogenesis of AP and subsequent inflammatory response. The genetic ablation of MIF or its inhibition with pharmacological agents significantly ameliorated the severity of AP. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

LncRNA HEIH promotes cell proliferation, migration and invasion in cholangiocarcinoma by modulating miR-98-5p/HECTD4
In conclusion, HEIH promoted CHOL tumorigenesis and progression by miR-98-5p/HECTD4 axis, which opens up a new insight for CHOL therapeutics.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

Multiple protein and mRNA expression correlations in the rat cerebral cortex after ischemic injury and repair due to buchang naoxintong jiaonang (BNJ) intervention
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Xin Liu, Qing Wang, Yiran Cui, Muhammad Hussain, Hongjun Yang, Xianyu LiAbstractStroke is the one of the most common causes of death worldwide. Systematic description and characterization of the types of stroke and the effects induced in the cerebral cortex have not been performed so far. Here, we analyzed the protein and mRNA expression in the cerebral cortex12 h after ischemic injury and repair. Drug intervention using Buchang Naoxintong Jiaonang (BNJ), which has been reported to have good clinical therapeutic effects, was selected ...
Source: Biomedicine and Pharmacotherapy - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

Comprehensive circRNA expression profile and construction of circRNA-related ceRNA network in cardiac fibrosis
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Xia Gu, Ya-Nan Jiang, Wei-Jie Wang, Jian Zhang, De-Si Shang, Chang-Bin Sun, Jiang-Tian Tian, Jin-Wei Tian, Bo Yu, Yao ZhangAbstractCardiac fibrosis is a common pathological condition that contributes to the progression of many cardiac diseases. Circular RNAs (circRNAs) are emerging as new regulators of cardiac fibrosis. However, the expression and function of circRNAs in cardiac fibrosis remain largely unknown. The present study aims to investigate the circRNA expression profile and identify the roles of circRNAs in cardiac fibrosis. ...
Source: Biomedicine and Pharmacotherapy - February 13, 2020 Category: Drugs & Pharmacology Source Type: research

A review on the potential of Resveratrol in prevention and therapy of diabetes and diabetic complications
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Dan-Dan Huang, Guangjiang Shi, Yaping Jiang, Chao Yao, Chuanlin ZhuAbstractDiabetes mellitus (DM) is a major world health problem and one of the most studied diseases, which are highly prevalent in the whole world, it is frequently associated with severe clinical complications, such as diabetic cardiomyopathy, nephropathy, retinopathy, neuropathy etc. Scientific research is continuously casting about for new monomer molecules from Chinese herbal medicine that could be invoked as candidate drugs for fighting against diabetes and its co...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

A network-regulative pattern in the pathogenesis of kidney injury following severe acute pancreatitis
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Qingqing Ruan, Hong Lu, Hengyue Zhu, Yangyang Guo, Yongheng BaiAbstractSevere acute pancreatitis (SAP), a critical inflammatory pathological disease of the pancreas, is crucial for the manifestation of lethal multiple organ dysfunction syndrome and systemic inflammatory response syndrome. Acute kidney injury (AKI) is one of the most severe complications of severe acute pancreatitis. Yet, the specific pathogenesis of AKI following SAP is defectively understood, and involves in multiple pathological processes in a “network-regulat...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Sanhuang Shu'ai decoction alleviates DSS-induced ulcerative colitis via regulation of gut microbiota, inflammatory mediators and cytokines
ConclusionSH significantly ameliorates the symptoms of DSS-induced colitis mice and the potential mechanism of SH may involve in multiple kinds of metabolic pathway including the regulation of gut microbiota, inflammatory mediators and cytokines.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

miR-6089/MYH9/β-catenin/c-Jun negative feedback loop inhibits ovarian cancer carcinogenesis and progression
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Longyang Liu, Yingxia Ning, Juanjuan Yi, Jianhuan Yuan, Weiyi Fang, Zhongqiu Lin, Zhaoyang ZengAbstractThe pathogenesis of ovarian cancer remains to be elucidated. Our previous study demonstrated that myosin heavy chain 9 (MYH9) overexpression was associated with poor prognosis of epithelial ovarian cancer. However, the mechanism of MYH9 and its regulation by microRNA (miR) is not clear. The results of the present study demonstrated that miR-6089 was one of the microRNAs targeting MYH9, and miR-6089 overexpression suppressed ovarian c...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Early prediction of tumor response after radiotherapy in combination with cetuximab in nasopharyngeal carcinoma using 99m Tc-duramycin imaging
This study aimed to investigate the potential of 99mTc-duramycin imaging in the early prediction of the curative effect of radiotherapy in combination with or without cetuximab in a nasopharyngeal carcinoma (NPC) model.MethodsMale BALB/c mice bearing NPC xenografts were randomized into four groups (six mice each group). Group 1 received radiotherapy (RT, 15 Gy/mouse) in combination with cetuximab (CTX, 2 mg/mouse), group 2 received RT (15 Gy/mouse), group 3 was treated using CTX (2 mg/mouse), and group 4, the control group, was treated using a vehicle. 99mTc-duramycin imaging was performed before treatment and 24 h after t...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

The anti-inflammatory effects of Pseudorlaric acid D on atherosclerosis
In this study, atherosclerosis model was established by feeding with a high-fat diet in ApoE−/− mice. PLAD was administered intragastrically at a dose of 5 mg/kg for four weeks. We found that PLAD could significantly improve the lipid metabolism and decrease atherosclerotic lesion areas as well as mitigate atherosclerotic changes on vessel walls. Besides, PLAD could markedly inhibit the inflammatory response by down-regulating the levels of Ly6Chi monocytes and NETs, and restraining NETs formation. The expression of pro-inflammatory cytokines IL-1β and IL-18 was also evidently reduced by PLAD. These result...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Overexpressed FOXO3 improves inflammatory status in mice by affecting NLRP3-mediated cell coronation in necrotizing colitis mice
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Yiyu Yin, Jian Wang, Xiaodong Zhao, Xiaole Wu, Huaxin Zou, Zhenfang Qin, Junhua CaoAbstractObjectiveTo investigate the relationship between FOXO3 overexpression and NLRP3 and explore the effect of FOXO3 on necrotizing colitis.Methods100 clean grade newborn SD (Sprague Dawley) rats were randomly divided into 4 groups: NEC group, NEC + FOXO3a group, NEC + NC group and control group. NEC rat model was established by hypoxia + hypothermia stimulation; HE staining was used for detection of the inflammation of intestinal tissue. The histolo...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

The protective effect of Ellagic acid (EA) in osteoarthritis: An in vitro and in vivo study
In this study, we reported the anti-inflammatory effects of Ellagic acid (EA) in the progression of OA in both in vitro and in vivo experiments. in vitro study, IL-1β-induced expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), Nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), and interleukin-6 (IL-6) were inhibited by Ellagic acid (EA). Moreover, Ellagic acid (EA) down-regulated the IL-1β-stimulated matrix metalloproteinase-13 (MMP-13) and thrombospondin motifs 5 (ADAMTS-5) while up-regulated the collagen of type II and aggrecan. Mechanistically, we...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Therapeutic effects of combination of platelet lysate and sulfasalazine administration in TNBS-induced colitis in rat
In conclusion, the combined administration of PL with conventional IBD therapy is able to effectively ameliorate IBD through modulation of inflammatory status.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Amelioration of experimental autoimmune encephalomyelitis by Rhodiola rosea, a natural adaptogen
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Xiuli Lin, Yingying Liu, Lili Ma, Xiaomeng Ma, Zhaoyu Chen, Hao Chen, Lei Si, Xueying Ma, Zhiling Yu, Xiaohong ChenAbstractAdaptogens are stress-response modifiers which have been considered to exert important influences on cytokines activities and immune response. Here, we investigated the effects of Rhodiola rosea (R. rosea), a natural adaptogen on the experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). We found that R. rosea alleviated the severity of EAE as evaluated by clinical and histopa...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Long non-coding RNA ASB16-AS1 enhances cell proliferation, migration and invasion via functioning as a ceRNA through miR-1305/Wnt/β-catenin axis in cervical cancer
ConclusionsThis study reveals that ASB16-AS1 promotes cell proliferation, migration, invasion via binding miR-1305 with Wnt2, and enhancing the Wnt/β-catenin pathway. ASB16-AS1 may play a new therapeutic target for CC. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

LncRNA LINC00173 enhances triple-negative breast cancer progression by suppressing miR-490-3p expression
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Huijie Fan, Jing Yuan, Xiangyu Li, Yihui Ma, Xiaofeng Wang, Benling Xu, Xingya LiAbstractLong non-coding RNA (lncRNA) LINC00173 has been previously shown to promote chemoresistance and progression of small-cell lung cancer. Herein, we examine the clinical significance and biological function of LINC00173 in triple-negative breast cancer (TNBC). Quantitative PCR analysis was performed to determine the expression of LINC00173 in TNBC and adjacent breast tissues (n = 84). The associations of LINC00173 expression with cancer features ...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

The long non-coding RNA MACC1-AS1 promotes nasopharyngeal carcinoma cell stemness via suppressing miR-145-mediated inhibition on SMAD2/MACC1-AS1 axis
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Shaoqing Chen, Xiao Luo, Wei Wu, Yong Li, Hongyuan Yu, Yurou Wang, Junfeng YanAbstractThe promoting roles of the long non-coding RNA (lncRNA) MACC1-AS1 have been indicated in gastric and pancreatic cancer, however, its roles in nasopharyngeal carcinoma (NPC) progression are never been revealed. In this work, it was shown that lncRNA MACC1-AS1 was highly expressed in NPC tissues and cells relative to the adjacent tissues and nasal mucosa cells, respectively. Additionally, MACC1-AS1 expression was positively correlated with the high rat...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Iridoids of Valeriana fauriei contribute to alleviating hepatic steatosis in obese mice by lipophagy
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Da-Hye Lee, So-Hyun Park, Yang Hoon Huh, Min Jung Kim, Hyo-Deok Seo, Tae-Youl Ha, Jiyun Ahn, Young-Jin Jang, Chang Hwa JungAbstractNonalcoholic fatty liver disease (NAFLD) is a common risk factor for metabolic syndrome that increases the risk of future cardiovascular disease, stroke, and diabetes. Recently, autophagy has been proposed as a means to prevent NAFLD. We investigated whether substances with autophagy-inducing activity alleviate NAFLD. The Valeriana fauriei (V. fauriei) was selected as a potential autophagy inducer among va...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Non‐coding RNA LOXL1-AS1 exhibits oncogenic activity in ovarian cancer via regulation of miR‐18b‐5p/VMA21 axis
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Fang Xue, Yan Hua Xu, Cheng Cheng Shen, Zhen Li Qin, Hai Bin ZhouAbstractLong non-coding RNAs (lncRNAs) exert critical effects in the process of malignant cancers and lncRNA LOXL1 Antisense RNA 1 (LOXL1-AS1) has been demonstrated to be a pro-oncogene in multiple tumor types. In the current study, we illuminated the precise roles of LOXL1-AS1 in the development of ovarian cancer. LOXL1-AS1 is significantly overexpressed in ovarian carcinoma tissue compared with adjacent non‐cancerous sample. The luciferase reporter gene assay reveals...
Source: Biomedicine and Pharmacotherapy - February 12, 2020 Category: Drugs & Pharmacology Source Type: research

Diabetic nephropathy execrates epithelial-to-mesenchymal transition (EMT) via miR-2467-3p/Twist1 pathway
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Yan Xu, Changhan Ouyang, Dayin Lyu, Zhangmei Lin, Wencai Zheng, Fan Xiao, Zhimin Xu, Lexi DingAbstractAlthough diabetic nephropathy (DN) is induced by a complicate interplay of multiple factors, the underlying mechanisms remain poorly characterized, even the treatment. Herein, we show that both of DN patients and STZ-induced type 1 diabetic rat exhibit the reduction both of urinary and circulating miR-2467-3p. We identify a negative correlation between miR-2467-3p levels and renal dysfunction. Administration of miR-2467-3p prevents di...
Source: Biomedicine and Pharmacotherapy - February 10, 2020 Category: Drugs & Pharmacology Source Type: research

ALDH1A1 maintains the cancer stem-like cells properties of esophageal squamous cell carcinoma by activating the AKT signal pathway and interacting with β-catenin
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Wei Wang, Shujin He, Renya Zhang, Jie Peng, Daolu Guo, Jinguo Zhang, Bo Xiang, Lei LiAbstractAldehyde dehydrogenase 1A1 (ALDH1A1) is a marker of cancer stem-like cells (CSCs), but knowledge about the molecular mechanism of ALDH1A1 in maintaining the properties of CSCs remains limited. ALDH1A1 immunohistochemistry was performed in esophageal squamous cell carcinoma (ESCC) tissues, Western blotting was used to detect relationship between ALDH1A1 and AKT or β-catenin. Subcutaneous transplantation of tumors and drug resistance, spher...
Source: Biomedicine and Pharmacotherapy - February 9, 2020 Category: Drugs & Pharmacology Source Type: research

Potential use of extract of the plant Schwartiza brasiliensis (choisy) bedell ex gir.-Cañas against the toxic effects of the venom of Bothrops jararaca or B. jararacussu
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Jenifer Frouche de Souza, Eduardo Coriolano de Oliveira, Ana Cláudia Rodrigues da Silva, Vagner Pereira da Silva, Maria Auxiliadora Coelho Kaplan, Maria Raquel Figueiredo, Eladio Flores Sanchez, André Lopes FulyAbstractEnvenomation by snakes is a worldwide health public issue, and antivenoms are less efficient in neutralizing local toxic effects. Thus, more efficient therapies to treat patients deserve attention, and plants have been extensively tested. So, the aim of this work was to evaluate the effect of the aqueous f...
Source: Biomedicine and Pharmacotherapy - February 9, 2020 Category: Drugs & Pharmacology Source Type: research

CircRBM33 regulates IL-6 to promote gastric cancer progression through targeting miR-149
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Ning Wang, Keyu Lu, Huiheng Qu, Hao Wang, Yigang Chen, Ting Shan, Xuhui Ge, Yunyu Wei, Peng Zhou, Jiazeng XiaAbstractThere is increasing evidence of the vital role played by circular RNAs (circRNAs) in the progression of gastric cancer (GC). A circRNA, hsa_circ_0001772, was generated from the RBM33 gene and named circRBM33. The aim of this study was to investigate the role of circRBM33 in GC. Quantitative real-time PCR (qRT-PCR) was used to quantify the expression of circRBM33 in 79 pairs of GC tissues and paracancerous tissues and 4 ...
Source: Biomedicine and Pharmacotherapy - February 9, 2020 Category: Drugs & Pharmacology Source Type: research

Silencing METTL3 inhibits the proliferation and invasion of osteosarcoma by regulating ATAD2
ConclusionOverall, our study revealed that METTL3 functions as an oncogene in the growth and invasion of osteosarcoma by regulating ATAD2, suggesting a potential therapeutic target for osteosarcoma treatment. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 9, 2020 Category: Drugs & Pharmacology Source Type: research

The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens
This study demonstrated that both the extracellular region and the intracellular tail of Crb2a are required to guide the formation of the apical zonula adherens.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 9, 2020 Category: Drugs & Pharmacology Source Type: research

Glucocorticoids induce osteoporosis mediated by glucocorticoid receptor-dependent and -independent pathways
This study reveals that GCs/Nr3c1 affect the expression of genes involved in bone metabolism and provides a basis to determine the role of GIOP and Nr3c1 in bone metabolism and development. We also identified a new effector target for the clinical treatment of GIOP. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 9, 2020 Category: Drugs & Pharmacology Source Type: research

The role of single strand break repair pathways in cellular responses to camptothecin induced DNA damage
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Chao Mei, Lin Lei, Li-Ming Tan, Xiao-Jing Xu, Bai-Mei He, Chao Luo, Ji-Ye Yin, Xi Li, Wei Zhang, Hong-Hao Zhou, Zhao-Qian LiuAbstractEfficient DNA repair is critical for cell survival following exposure to DNA topoisomerase I (Top1) inhibitors camptothecin, a nature product from which the common chemotherapeutic drugs irinotecan and topotecan are derived. The camptothecin-derived agents exert their antitumor activities by specifically stabilizing the Top1–DNA covalent complexes (Top1cc) and blocking the DNA religation step. When...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

MiR-30c-5p mediates the effects of panax notoginseng saponins in myocardial ischemia reperfusion injury by inhibiting oxidative stress-induced cell damage
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Linli Wang, Xiaoqian Chen, Yingchao Wang, Lu Zhao, Xiaoping Zhao, Yi WangAbstractMyocardial ischemia reperfusion (MI/R) injury is a severe pathological process that threatens human health all over the world. The role of microRNAs (miRNAs) in the pathogenesis of MI/R injury has been increasingly recognized in recent years. Here, we conducted a miRNA profiling of the hearts of MI/R injured rat model, and identified 46 miRNAs which were differentially expressed between the MI/R injury and the control groups. With a special focus on one o...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Ruscogenin alleviates LPS-induced pulmonary endothelial cell apoptosis by suppressing TLR4 signaling
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Yunhao Wu, Yuwei Wang, Shuaishuai Gong, Jiahui Tang, Jiazhi Zhang, Fang Li, Boyang Yu, Yuanyuan Zhang, Junping KouAbstractAcute lung injury (ALI) or its most advanced form, acute respiratory distress syndrome (ARDS) is a severe inflammatory pulmonary process triggered by varieties of pathophysiological factors, among which apoptosis of pulmonary endothelial cells plays a critical role in the progression of ALI/ARDS. Ruscogenin (RUS) has been found to exert significant protective effect on ALI induced by lipopolysaccharides (LPS), but ...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Total phenolic extract of Euscaphis konishii hayata Pericarp attenuates carbon tetrachloride (CCl4)-induced liver fibrosis in mice
In conclusion, TPEP inhibited CCl4-induced hepatic fibrosis through its antioxidant, anti-inflammatory and anti-fibrotic activities. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Targeting SGK1 enhances the efficacy of radiotherapy in locally advanced rectal cancer
In conclusion, our findings indicate that SGK1 is a key player in LARC radioresistance, and drives radioresistance in an ATF3 dependent manner, which provides insights for future radio-sensitizer design.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Soufeng Yuchuan decoction mitigates the ovalbumin-induced lung damage in a rat model of asthma
This study aimed to investigate the effect of Soufeng Yuchuan (SFYC) decoction, a Traditional Chinese Medicine, on airway remodeling and expression of VEGF and TGF-β1 in asthma model rats. A rat model of asthma was induced by ovalbumin (OVA) treatment. The results showed that SFYC decoction improved general conditions and reduced the damage in lung tissues in asthma model rats. Furthermore, SFYC decoction significantly reduced the OVA-induced levels of VEGF and TGF-β1 in sera and in bronchoalveolar lavage fluid. Moreover, SFYC decoction decreased the OVA-induced VEGF mRNA and protein levels in lung tissues in ast...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Altered serum microRNA expression profile in subjects with heroin and methamphetamine use disorder
ConclusionsOur study raises the possibility that the altered serum miRNAs could potentially be used as an auxiliary tool to identify individuals in drug abuse and addiction.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5
In this study, we demonstrated that miR-645 knockdown significantly diminished CRC cell migration and invasion and repressed epithelial–mesenchymal transition (EMT). Conversely, miR-645 overexpression enhanced CRC cell migration, invasion, and EMT. In vivo assays confirmed that miR-645 knockdown substantially reduced CRC growth and metastasis. Regarding the mechanism, ephrin-A5 (EFNA5) was identified as a direct target gene of miR-645. MiR-645 specifically targeted the 3′-untranslated region of EFNA5 mRNA and hindered its expression. EFNA5 knockdown attenuated the effects of miR-645 knockdown on CRC cell migrat...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Trelagliptin succinate: DPP-4 inhibitor to improve insulin resistance in adipocytes
In conclusion, our study suggested that trelagliptin succinate improved insulin resistance in adipocytes via regulation of PI-3K/AKT/GLUT4 insulin signaling pathway.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Elevated SRC3 expression predicts pemetrexed resistance in lung adenocarcinoma
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Yu Chen, Yan Sun, Wei Zhao, Yongfu Ma, Zhifeng Yan, Xiuhong NieAbstractLung cancer has been the leading cause of cancer-related death for many years worldwide. Pemetrexed, either as monotherapy or combined with other agents, is the preferred chemotherapy regimen for lung adenocarcinoma. However, both de novo and acquired resistance against pemetrexed frequently occur and lead to poor prognosis of patients. The underlying mechanisms remain poorly characterized. Here, RNA-seq analysis is utilized to compare gene expression levels in an ...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

PolyG mitigates silica-induced pulmonary fibrosis by inhibiting nucleolin and regulating DNA damage repair pathway
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Qiang Zhou, Yi Guan, Ruiyan Hou, Junnan Wang, Hongsheng Gao, Haibin Li, Yingzheng Zhao, Nan Liu, Yongheng Wang, Ning Li, Sanqiao YaoAbstractPolyguanylic acid potassium salt (PolyG) has an anti-fibrotic G-quadruplex (G4) structure. It could inhibit the expression of nucleolin, a protein involved in cell proliferation and apoptosis. However, its role in regulating nucleolin in silicosis is still unknown. After instillation of 50 μl of crystalline silica suspension (50 mg/ml) into the trachea of C57BL/6 mice, we show that nucleolin ex...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Profile analysis reveals endogenous RNAs regulate necrotizing enterocolitis progression
In conclusion, our study revealed that these differentially expressed lncRNAs may participate in the development of NEC via interactions with miRNAs and may serve as possible biomarkers and target genes in NEC. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Knockdown of enhancer of rudimentary homolog inhibits proliferation and metastasis in ovarian cancer by regulating epithelial-mesenchymal transition
In this study, immunohistochemical staining revealed that the protein expression of ERH was associated with histological type, lymph node metastasis and pathological grade in EOC patients. To verify the association of ERH with the prognosis of OC, a GSE microarray dataset was downloaded from the Gene Expression Omnibus (GEO) database. Survival analysis suggested that ERH may be associated with poor prognosis of OC. In addition, shRNA was used to knockdown the protein and mRNA expression levels of ERH in the OC cell line SKOV3. Inhibition of ERH expression slowed proliferation, promoted apoptosis and inhibited metastasis an...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Carvacrol protects against diabetes-induced hypercontractility in the aorta through activation of the PI3K/Akt pathway
In conclusion, our results strongly suggest that CAR plays a protective role in diabetes-induced aortic hypercontractility, possibly by activating the PI3K/Akt signaling pathway. CAR is a potential drug for the treatment of diabetic vasculopathy. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Multi-omics analysis at epigenomics and transcriptomics levels reveals prognostic subtypes of lung squamous cell carcinoma
In this study, we identified prognostic biomarkers for lung squamous cell carcinoma (LUSC) by integrating multiple sets of DNA copy number variants (CNV) and methylation variant (MET) data, and performing qPCR and immunohistochemical identification. We examined the expression of CNV and MET in 368 LUSC patients. Gene expression associated with DNA copy number or DNA methylation was identified and four LUSC gene subtypes were defined based on these correlations. The prognosis overall survival (OS) of the iC1 subtype was significantly lower than that in the iC2 and iC4 subtypes. We assessed the immune scores of each subtype ...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

EFNB2 facilitates cell proliferation, migration, and invasion in pancreatic ductal adenocarcinoma via the p53/p21 pathway and EMT
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Feng Zhu, Shang-Nan Dai, Da-Lai Xu, Chao-Qun Hou, Tong-Tai Liu, Qiu-Yang Chen, Jun-Li Wu, Yi MiaoAbstractEphrin-2 (EFNB2) is expressed at abnormally high levels in some neoplasms, such as squamous cell carcinoma of the head and neck and colorectal cancer. Its overexpression is associated with the malignant progression of tumors. However, the expression of EFNB2 in pancreatic ductal adenocarcinoma (PDAC) has not been thoroughly studied. EFNB2 expression was evaluated by quantitative real-time PCR, immunohistochemistry, and western blot...
Source: Biomedicine and Pharmacotherapy - February 7, 2020 Category: Drugs & Pharmacology Source Type: research

Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice
ConclusionsThe anti-diabetic effect of 14 probiotics in db/db mice seem to be related to an increase of SCFA-producing bacteria, the improvement of intestinal barrier function and the upregulation of GLP-1 production, and indicate these probiotics might be a good candidate to prevent and treat diabetes.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 6, 2020 Category: Drugs & Pharmacology Source Type: research

CDK4/RB/E2Fs axis as potential therapeutic target of endometrial cancer
In this study, we found that the pathways associated with mitotic cell cycle were enriched in primary EC samples versus normal endometrial samples through analyzing RNA-seq data of The Cancer Genome Atlas (TCGA). The messenger RNA (mRNA) expression of three activator E2Fs (E2F1, E2F2, and E2F3) and their target genes increased significantly in EC samples. Additionally, the high transcriptional activity of activator E2Fs was associated with poor survival, advanced clinical stage, high histologic grade, and aggressive histological type. We further demonstrated that E2Fs hyperactivation correlated with DNA hypomethylation and...
Source: Biomedicine and Pharmacotherapy - February 6, 2020 Category: Drugs & Pharmacology Source Type: research

Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats
ConclusionThe combinatorial treatment of SNX and captopril lowered blood pressure through adjusting NO/NOS, ET-1 and dyslipidemia profile. Furthermore, this treatment alleviated the kidney damage via reducing the release of inflammatory factors and the expression of apoptotic markers. Therefore, these results provided a rationale for future clinical use of SNX combined with captopril in antihypertensive and protecting renal functions in hypertension. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - February 6, 2020 Category: Drugs & Pharmacology Source Type: research

Protective effects and mechanisms of Rehmannia glutinosa leaves total glycoside on early kidney injury in db/db mice
Publication date: May 2020Source: Biomedicine & Pharmacotherapy, Volume 125Author(s): Zhuo Xu, Xin-xin Dai, Qing-yang Zhang, Shu-lan Su, Hui Yan, Yue Zhu, Er-xin Shang, Da-wei Qian, Jin-ao DuanAbstractThe spontaneous db/db mice were used to elucidate the biological effects and mechanisms of Rehmannia glutinosa leaves total glycoside (DHY) on kidney injury through biochemical indicators, kidney pathological section analysis, metabolic profiling, intestinal flora analysis and in vitro Human renal tubular epithelial (HK-2) cell model induced by high glucose. It was found that DHY can decrease the blood sugar level (insuli...
Source: Biomedicine and Pharmacotherapy - February 4, 2020 Category: Drugs & Pharmacology Source Type: research