Validation of hepatobiliary transport PET imaging in liver function assessment: Evaluation of 3 β-[18F]FCA in mouse models of liver disease
Recently, our research group reported on the development of 3 β-[18F]Fluorocholic acid (3β-[18F]FCA), a 18F labeled bile acid to detect drug interference with the bile acid transporters (drug-induced cholestasis). It was hypothesized that 3β-[18F]FCA could also be used as a non-invasive tool to monitor (regional) liver function in vivo in different liver di seases through altered expression of bile acid transporters. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - December 7, 2018 Category: Nuclear Medicine Authors: Stef De Lombaerde, Lindsey Devisscher, Jeroen Verhoeven, Sara Neyt, Hans Van Vlierberghe, Christian Vanhove, Filip De Vos Source Type: research
In Vivo imaging characterization and anticancer efficacy of a novel HER2 Affibody and Pemetrexed conjugate in lung Cancer model
In this study, a new agent consisting of HER2-specific affibody ZHER2:V2 and chemotherapy drug pemetrexed was synthesized to develop a new targeted drug. Its biological characteristics and anticancer efficacy were assessed in cells level and xenografts models by radiolabeling with technetium-99 m. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - November 28, 2018 Category: Nuclear Medicine Authors: Honglei Jiao, Xinming Zhao, Jiahui Liu, Tuo Ma, Zhaoqi Zhang, Jingmian Zhang, Jianfang Wang Source Type: research
68Ga-labelled NOTA-RGD-GE11 peptide for dual integrin and EGFR-targeted tumour imaging
Multiple peptide receptors are co-rexpressed in many types of cancers. Arg-Gly-Asp (RGD) and GE11 peptides specifically target integrin αVβ3 and EGFR, respectively. Recently, we designed and synthesized a heterodimer peptide NOTA-c(RGDyK)-GE11 (NOTA-RGD-GE11). The aim of this study was to investigate the characteristics of NOTA-RGD-GE11 for dual receptor imaging. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - November 27, 2018 Category: Nuclear Medicine Authors: Chien-Jen Chen, Chen-Hsin Chan, Kun-Liang Lin, Jyun-Hong Chen, Chun-Hao Tseng, Ping-Yen Wang, Chuan-Yi Chien, Hung-Man Yu, Wuu-Jyh Lin Source Type: research
Synthesis, characterization and biological studies of rhenium, Technetium-99 m and Rhenium-188 Pentapeptides
This study shows the synthesis and characterization of KYCAR complexes containing nonradioactive rhenium, 99mTc, or 188Re. The metal complexes were also biologically evaluated to determine in vivo distribution in healthy mice. The overall goals of this project were (1) to synthesize the Tc/Re pentapeptide complexes, (2) to identify spectroscopic methods for characterization of syn versus anti rhenium peptide complexes, (3) to analyze the ex vivo stability, and (4) to assess the biological properties of the [99mTc]TcO-KYCAR and [188Re]ReO-KYCAR complexes in vivo. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - November 12, 2018 Category: Nuclear Medicine Authors: Vanessa A. Sanders, David Iskhakov, Dalya Abdel-Atti, Matthew Devany, Michelle C. Neary, Ken R. Czerwinski, Lynn C. Francesconi Source Type: research
Development of [18F]FAMTO: A novel fluorine-18 labelled positron emission tomography (PET) radiotracer for imaging CYP11B1 and CYP11B2 enzymes in adrenal glands
Primary aldosteronism accounts for 6 –15% of hypertension cases, the single biggest contributor to global morbidity and mortality. Whilst ~50% of these patients have unilateral aldosterone-producing adenomas, only a minority of these have curative surgery as the current diagnosis of unilateral disease is poor. Carbon-11 radiolabelled metomidate ([11C]MTO) is a positron emission tomography (PET) radiotracer able to selectively identify CYP11B1/2 expressing adrenocortical lesions of the adrenal gland. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - November 9, 2018 Category: Nuclear Medicine Authors: Salvatore Bongarzone, Filippo Basagni, Teresa Sementa, Nisha Singh, Caleb Gakpetor, Vincent Faugeras, Jayanta Bordoloi, Antony D. Gee Source Type: research
Editorial Board
(Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - November 1, 2018 Category: Nuclear Medicine Source Type: research
Table of Contents & Barcode
(Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - November 1, 2018 Category: Nuclear Medicine Source Type: research
Tumor uptake and tumor/blood ratios for 89Zr-DFO-trastuzumab-DM1 on microPET/CT images in NOD/SCID mice with human breast cancer xenografts are directly correlated with HER2 expression and response to trastuzumab-DM1
Our objective was to determine correlations between the tumor uptake and T/B ratios for 89Zr-labeled T-DM1 (89Zr-DFO-T-DM1) in mice with human BC xenografts by microPET/CT and biodistribution studies with HER2 expression and response to treatment with trastuzumab-DM1 (T-DM1). (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - October 16, 2018 Category: Nuclear Medicine Authors: Noor Al-saden, Zhongli Cai, Raymond M. Reilly Source Type: research
Al18F-complexation of DFH17, a NOTA-conjugated adrenomedullin analog, for PET imaging of pulmonary circulation
Adrenomedullin receptors are highly expressed in human alveolar capillaries and provide a molecular target for imaging the integrity of pulmonary microcirculation. In this work, we aimed to develop a NOTA-derivatized adrenomedullin analog (DFH17), radiolabeled with [18F]AlF, for PET imaging of pulmonary microcirculation. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - October 16, 2018 Category: Nuclear Medicine Authors: Luis Michel Alonso Martinez, Fran çois Harel, Quang T. Nguyen, Myriam Létourneau, Caroline D'Oliviera-Sousa, Bernard Meloche, Vincent Finnerty, Alain Fournier, Jocelyn Dupuis, Jean N. DaSilva Source Type: research
Unexpected decrease in in vivo binding of [3H]QNB in the mouse cerebral cortex in the developing brain - a comparison with [11C]NMPB
Significant discrepancies between in vitro and in vivo binding of the muscarinic receptor ligand - 3H-labeled Quinuclidinyl Benzylate (QNB) - have been well documented. Discernable in vivo cerebellar [3H]QNB binding has been observed in mouse brain, despite the maximum number of binding sites (Bmax) being low. In order to understand this unique in vivo binding phenomenon, the binding of two muscarinic receptor ligands -[3H]QNB and N-[11C]methylpiperidyl Benzylate ([11C]NMPB) - were compared in vivo and in vitro in 3- and 8-week-old mice. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - October 16, 2018 Category: Nuclear Medicine Authors: Osamu Inoue, Toshiyuki Sato, Kaoru Kobayashi, Antony Gee, Miho Shukuri, Ming-Rong Zhang Source Type: research
Efficient cartridge purification for producing high molar activity 18F-glycoconjugates via oxime formation
18F-fluoroglycosylation via oxime formation is a chemoselective and mild radiolabeling method for sensitive molecules. Glycosylation can also improve the bioavailability, in vivo kinetics, and stability of the compound in blood, as well as accelerate clearance of biomolecules. A typical synthesis procedure for 18F-fluoroglycosylation with [18F]FDG (2-deoxy-2-[18F]fluoro-d-glucose) and [18F]FDR (5-deoxy-5-[18F]fluoro-d-ribose) involves two HPLC (high performance liquid chromatography) purifications: one after 18F-fluorination of the carbohydrate to remove its labeling precursor, and a second one after the oxime formation st...
Source: Nuclear Medicine and Biology - October 15, 2018 Category: Nuclear Medicine Authors: Outi Kein änen, Denisa Partelová, Osku Alanen, Maxim Antopolsky, Mirkka Sarparanta, Anu J. Airaksinen Source Type: research
Automated radiosynthesis of 5-[11C]l-glutamine, an important tracer for glutamine utilization
The natural amino acid l-Glutamine (Gln) is essential for both cell growth and proliferation. In addition to glucose, cancer cells utilize Gln as a carbon source for ATP production, biosynthesis, and as a defense against reactive oxygen species. The utilization of [11C]Gln has been previously reported as a biomarker for tissues with an elevated demand for Gln, however, the previous reports for the preparation of [11C]Gln were found to be lacking several crucial aspects necessary for transition to human production. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - October 15, 2018 Category: Nuclear Medicine Authors: Adam J. Rosenberg, Michael L. Nickels, Michael L. Schulte, H. Charles Manning Source Type: research
Molar activity – the keystone in 11C-radiochemistry: an explorative study using the gas phase method
Radiochemists/radiopharmacists, involved in the preparation of radiopharmaceuticals are regularly confronted with the requirement of continuous high quality productions in their day-to-day business. One of these requirements is high specific or molar activity of the radiotracer in order to avoid e.g. receptor saturation and pharmacological or even toxic effects of the applied tracer for positron emission tomography. In the case of 11C-labelled radiotracers, the reasons for low molar activity are manifold and often the search for potential 12C-contaminations is time-consuming. (Source: Nuclear Medicine and Biology)
Source: Nuclear Medicine and Biology - October 12, 2018 Category: Nuclear Medicine Authors: Verena Pichler, Thomas Zenz, C écile Philippe, Chrysoula Vraka, Neydher Berrotéran-Infante, Sarah Pfaff, Lukas Nics, Marius Ozenil, Oliver Langer, Matthäus Willeit, Tatjana Traub-Weidinger, Rupert Lanzenberger, Markus Mitterhauser, Marcus Hacker, Wolfg Source Type: research
[18F]F-DPA for the detection of activated microglia in a mouse model of Alzheimer's disease
Neuroinflammation is associated with several neurological disorders, including Alzheimer's disease (AD). The translocator protein 18 kDa (TSPO), due to its overexpression during microglial activation and relatively low levels in the brain under normal neurophysiological conditions, is commonly used as an in vivo biomarker for neuroinflammation. Reported here is the preclinical evaluation of [18F]F-DPA, a promising new TSPO-spe cific radioligand, as a tool for the detection of activated microglia at different ages in the APP/PS1–21 mouse model of AD and a blocking study to determine the specificity of the tracer. (Sour...
Source: Nuclear Medicine and Biology - September 26, 2018 Category: Nuclear Medicine Authors: Thomas Keller, Francisco R. L ópez-Picón, Anna Krzyczmonik, Sarita Forsback, Anna K. Kirjavainen, Jatta S. Takkinen, Obada Al-Zghool, Johan Rajander, Simo Teperi, Fanny Cacheux, Annelaure Damont, Frédéric Dollé, Juha O. Rinne, Olof Solin, Merja Haapa Source Type: research
Issues in preclinical radiopharmaceutical research: Significance, relevance and reproducibility
The use of radiolabeled compounds for in vivo preclinical animal studies – broadly considered here as the quantification of the distribution of radioactivity after administration of a radiolabeled chemical to a living animal such as a rodent or nonhuman primate – has steadily increased in recent years, becoming a much more widely used method for studying biochemistry , physiology and pharmacology. Although once a narrow field with results published in a handful of specialty journals in radiology and nuclear medicine, the methods utilized for preclinical in vivo radiotracer studies have now become more accepted and pape...
Source: Nuclear Medicine and Biology - September 3, 2018 Category: Nuclear Medicine Authors: Michael R. Kilbourn, Peter J.H. Scott Source Type: research
