Ghosts of parasites past influence current non-consumptive effects in Drosophila nigrospiracula
Int J Parasitol. 2024 Apr 25:S0020-7519(24)00078-X. doi: 10.1016/j.ijpara.2024.04.008. Online ahead of print.ABSTRACTParasites can indirectly impact hosts through non-consumptive effects (NCEs) via changes in behaviour, morphology, and/or physiology. These responses can be understood in terms of the ecology of fear (ectoparasites) or the ecology of disgust (endoparasites) framework. We tested the hypothesis that NCEs of parasite exposure (e.g., parasite avoidance and defense) trade off with other important behaviours such as feeding and resting. We predicted that when exposed to parasites (without infection), hosts will in...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: C Liang L T Luong Source Type: research
Novel CRISPR-Cas-powered pen-side test for East Coast fever
We describe a streamlined, field-applicable diagnostic tool comprising a 20 min recombinase polymerase amplification (RPA) reaction followed by a 60 min CRISPR-Cas12a reaction using a FAM/Biotin lateral flow strip readout. We tested two different RPA primer pairs and four different CRISPR-RNAs (crRNAs). The p104-based assay displayed high sensitivity, detecting as low as one infected lymphocyte per three microliters of blood and universally detecting eight different T. parva strains without detecting DNA from other Theileria spp. such as Theileria mutans and Theileria lestoquardi. This work opens the way for a field-applic...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: Robert Muriuki Maingi Ndichu Samuel Githigia Nicholas Svitek Source Type: research
Hookworm genomics: dusk or dawn?
Trends Parasitol. 2024 Apr 26:S1471-4922(24)00085-0. doi: 10.1016/j.pt.2024.04.003. Online ahead of print.ABSTRACTHookworms are parasites, closely related to the model nematode Caenorhabditis elegans, that are a major economic and health burden worldwide. Primarily three hookworm species (Necator americanus, Ancylostoma duodenale, and Ancylostoma ceylanicum) infect humans. Another 100 hookworm species from 19 genera infect primates, ruminants, and carnivores. Genetic data exist for only seven of these species. Genome sequences are available from only four of these species in two genera, leaving 96 others (particularly thos...
Source: Trends in Parasitology - April 27, 2024 Category: Parasitology Authors: Vladislav Il ík Erich M Schwarz Eva Noskov á Barbora Paf čo Source Type: research
Optimization of benzenesulfonyl derivatives as anti-Trypanosomatidae agents: Structural design, synthesis, and pharmacological assessment against Trypanosoma cruzi and Leishmania infantum
This study details the early stages of hit-to-lead optimization for a benzenesulfonyl derivative, denoted as initial hit, against Trypanossoma cruzi (T. cruzi), Leishmania infantum (L. infantum) and Leishmania braziliensis (L. braziliensis). We investigated structure - activity relationships using a series of 26 newly designed derivatives, ultimately yielding potential lead candidates with potent low-micromolar and sub-micromolar activities against T. cruzi and Leishmania spp, respectively, and low in vitro cytotoxicity against mammalian cells. These discoveries emphasize the significant promise of this chemical class in t...
Source: Bioorganic and Medicinal Chemistry - April 27, 2024 Category: Chemistry Authors: Guilherme Freitas de Lima Hercos Mariza Gabriela Faleiro de Moura Lodi Cruz Ana Clara Cassiano Martinho Daniela de Melo Resende Danilo Farago Nascimento Paula Derksen Macruz Eduardo Jorge Pilau Silvane Maria Fonseca Murta Celso de Oliveira Rezende J únio Source Type: research
To Explore Potential Inhibitors Against Various Enzymatic Targets of Human African Trypanosomiasis
Comb Chem High Throughput Screen. 2024 Apr 25. doi: 10.2174/0113862073293708240416113543. Online ahead of print.ABSTRACTSynthetic drugs currently prescribed for the treatment of Human African Trypanosomiasis (HAT) are non-specific, toxic, demand extended therapeutic regimes and are of varying efficacy. Along with the challenging demographic and socio-economic hurdles, the everincreasing risk of drug resistance is another major problem to be addressed. Cysteine protease, Heat shock proteins (HSP-90), Trypanothione reductase (TR), Farnesyl diphosphate synthase, Glucose-6-phosphate dehydrogenase, UP-4-galactose epimerase, and...
Source: Combinatorial Chemistry and High Throughput Screening - April 27, 2024 Category: Chemistry Authors: Sayandeep Brahma Susmita Moitra Soumya Ranjan Bagchi Asmita Samadder Sisir Nandi Source Type: research
Genetic Diversity of Blastocystis in Diarrheal Cases: Identification of Subtypes and Alleles
In conclusion, our study provides important data on the molecular epidemiological characteristics of the Blastocystis by determining positivity, subtypes and alleles in diarrheal cases. Therefore, within the scope of the one health approach, comprehensive molecular epidemiological studies are required to determine the presence and genotypes of Blastocystis in human, animal and environmental samples.PMID:38676586 | DOI:10.5578/mb.202498207 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Evren Tileklio ğlu Hatice Ertabaklar Source Type: research
Hookworm genomics: dusk or dawn?
Trends Parasitol. 2024 Apr 26:S1471-4922(24)00085-0. doi: 10.1016/j.pt.2024.04.003. Online ahead of print.ABSTRACTHookworms are parasites, closely related to the model nematode Caenorhabditis elegans, that are a major economic and health burden worldwide. Primarily three hookworm species (Necator americanus, Ancylostoma duodenale, and Ancylostoma ceylanicum) infect humans. Another 100 hookworm species from 19 genera infect primates, ruminants, and carnivores. Genetic data exist for only seven of these species. Genome sequences are available from only four of these species in two genera, leaving 96 others (particularly thos...
Source: Trends in Parasitology - April 27, 2024 Category: Parasitology Authors: Vladislav Il ík Erich M Schwarz Eva Noskov á Barbora Paf čo Source Type: research
The Development of < em > Toxoplasma gondii < /em > Recombinant Trivalent Chimeric Proteins as an Alternative to < em > Toxoplasma < /em > Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants
This study presents an evaluation of seventeen newly produced recombinant trivalent chimeric proteins (containing the same immunodominant fragment of SAG1 and SAG2 of Toxoplasma gondii antigens, and an additional immunodominant fragment of one of the parasite antigens, such as AMA1, GRA1, GRA2, GRA5, GRA6, GRA7, GRA9, LDH2, MAG1, MIC1, MIC3, P35, and ROP1) as a potential alternative to the whole-cell tachyzoite lysate (TLA) used in the detection of infection in small ruminants. These recombinant proteins, obtained by genetic engineering and molecular biology methods, were tested for their reactivity with specific anti-Toxo...
Source: Molecular Medicine - April 27, 2024 Category: Molecular Biology Authors: Bart łomiej Tomasz Ferra Maciej Chyb Karolina So łowińska Lucyna Holec-G ąsior Marta Skwarecka Karolina Baranowicz Justyna Gatkowska Source Type: research
Oxfendazole Nitazoxanide combination in experimental neurocysticercosis - anti-inflammatory and cysticidal effects
Exp Parasitol. 2024 Apr 25:108764. doi: 10.1016/j.exppara.2024.108764. Online ahead of print.ABSTRACTNeurocysticercosis (NCC) is a parasitic infection caused by the larval stage of the pork tapeworm, Taenia solium. The complications of NCC include seizures, headaches, cognitive impairment, and focal neurological deficits. In addition to antiparasitic drugs and surgery, the management of NCC includes the use of corticosteroids to reduce inflammation and control symptoms. The traditional treatment with albendazole and praziquantel has not been altered over 30 years and present several side effects. There are other anti-helmi...
Source: Experimental Parasitology - April 27, 2024 Category: Parasitology Authors: Jefferson Aber Alves Ara újo Taynara Cristina Gomes Vanessa Cindy Neres Lima Yngrid Batista da Silva Ruy de Souza Lino Junior Marina Clare Vinaud Source Type: research
Novel CRISPR-Cas-powered pen-side test for East Coast fever
We describe a streamlined, field-applicable diagnostic tool comprising a 20 min recombinase polymerase amplification (RPA) reaction followed by a 60 min CRISPR-Cas12a reaction using a FAM/Biotin lateral flow strip readout. We tested two different RPA primer pairs and four different CRISPR-RNAs (crRNAs). The p104-based assay displayed high sensitivity, detecting as low as one infected lymphocyte per three microliters of blood and universally detecting eight different T. parva strains without detecting DNA from other Theileria spp. such as Theileria mutans and Theileria lestoquardi. This work opens the way for a field-applic...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: Robert Muriuki Maingi Ndichu Samuel Githigia Nicholas Svitek Source Type: research
Ghosts of parasites past influence current non-consumptive effects in Drosophila nigrospiracula
Int J Parasitol. 2024 Apr 25:S0020-7519(24)00078-X. doi: 10.1016/j.ijpara.2024.04.008. Online ahead of print.ABSTRACTParasites can indirectly impact hosts through non-consumptive effects (NCEs) via changes in behaviour, morphology, and/or physiology. These responses can be understood in terms of the ecology of fear (ectoparasites) or the ecology of disgust (endoparasites) framework. We tested the hypothesis that NCEs of parasite exposure (e.g., parasite avoidance and defense) trade off with other important behaviours such as feeding and resting. We predicted that when exposed to parasites (without infection), hosts will in...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: C Liang L T Luong Source Type: research
Occurrence of parasites in waters used for crops irrigation and vegetables from the Savannah of Bogot á, Colombia
In this study, the presence of parasites in surface water used for irrigation of crops (n = 96) and vegetables (celery and lettuce) (n = 120), from the Former La Ramada irrigation district, was evaluated using conventional and molecular parasitological methods. Our findings showed contamination of irrigation systems in the study area with domestic wastewater, demonstrated by the presence of Ancylostomatidae eggs, Ascaris spp., Hymenolepis spp., Trichuris spp., Capillaria spp., Giardia spp. cysts, and oocysts of Toxoplasma gondii and Cryptosporidium spp. A prevalence of 33% and 23.3% was calculated for helminths and protozo...
Source: Environmental Science and Pollution Research International - April 27, 2024 Category: Environmental Health Authors: Daniel Eduardo Ospina Santos Yulieth Alexandra Upegui Zapata Catherine Aguilar Buitrago Geraldine S ánchez Herrera Libia Eunise Chandillo Becoche Myriam Consuelo L ópez Páez Martha Cristina Bustos L ópez Carolina Ortiz Pineda Source Type: research
Optimization of benzenesulfonyl derivatives as anti-Trypanosomatidae agents: Structural design, synthesis, and pharmacological assessment against Trypanosoma cruzi and Leishmania infantum
This study details the early stages of hit-to-lead optimization for a benzenesulfonyl derivative, denoted as initial hit, against Trypanossoma cruzi (T. cruzi), Leishmania infantum (L. infantum) and Leishmania braziliensis (L. braziliensis). We investigated structure - activity relationships using a series of 26 newly designed derivatives, ultimately yielding potential lead candidates with potent low-micromolar and sub-micromolar activities against T. cruzi and Leishmania spp, respectively, and low in vitro cytotoxicity against mammalian cells. These discoveries emphasize the significant promise of this chemical class in t...
Source: Bioorganic and Medicinal Chemistry - April 27, 2024 Category: Chemistry Authors: Guilherme Freitas de Lima Hercos Mariza Gabriela Faleiro de Moura Lodi Cruz Ana Clara Cassiano Martinho Daniela de Melo Resende Danilo Farago Nascimento Paula Derksen Macruz Eduardo Jorge Pilau Silvane Maria Fonseca Murta Celso de Oliveira Rezende J únio Source Type: research
To Explore Potential Inhibitors Against Various Enzymatic Targets of Human African Trypanosomiasis
Comb Chem High Throughput Screen. 2024 Apr 25. doi: 10.2174/0113862073293708240416113543. Online ahead of print.ABSTRACTSynthetic drugs currently prescribed for the treatment of Human African Trypanosomiasis (HAT) are non-specific, toxic, demand extended therapeutic regimes and are of varying efficacy. Along with the challenging demographic and socio-economic hurdles, the everincreasing risk of drug resistance is another major problem to be addressed. Cysteine protease, Heat shock proteins (HSP-90), Trypanothione reductase (TR), Farnesyl diphosphate synthase, Glucose-6-phosphate dehydrogenase, UP-4-galactose epimerase, and...
Source: Combinatorial Chemistry and High Throughput Screening - April 27, 2024 Category: Chemistry Authors: Sayandeep Brahma Susmita Moitra Soumya Ranjan Bagchi Asmita Samadder Sisir Nandi Source Type: research
Genetic Diversity of Blastocystis in Diarrheal Cases: Identification of Subtypes and Alleles
In conclusion, our study provides important data on the molecular epidemiological characteristics of the Blastocystis by determining positivity, subtypes and alleles in diarrheal cases. Therefore, within the scope of the one health approach, comprehensive molecular epidemiological studies are required to determine the presence and genotypes of Blastocystis in human, animal and environmental samples.PMID:38676586 | DOI:10.5578/mb.202498207 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Evren Tileklio ğlu Hatice Ertabaklar Source Type: research
