Specific Tests and Inflammatory Biomarkers in the Evaluation of Brucellosis Disease
In this study, it was aimed to determine the relationship between laboratory parameters and clinical response and organ involvement in patients with Brucellosis diagnosed with specific diagnostic tests. In the study, 100 patients without previous diagnosis of Brucellosis who have admitted to the Department of Infectious Diseases and Clinical Microbiology and had positive Brucella tube agglutination tests and whose clinic was compatible with Brucellosis were evaluated prospectively. Patients were invited to be checked in the 1st, 2nd and 6th weeks . Patients with hip pain and low back pain were evaluated with sacroiliac mag...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ferhan Kerget Bu ğra Kerget Neslihan Çelik Sibal İba Yılmaz Source Type: research

Effect of N-acetylcystein on Antibiofilm Efficiency of Antibiotics in Staphylococci Isolates
In this study, it was aimed to demonstrate i) the biofilm-forming abilities ii) the change in ampicillin and vancomycin MIC values in the presence of NAC molecules, iii) the change in the MBEK values of these antibiotics in the presence of NAC molecule and iv) the change in the expression levels of genes thought to be related to biofilm formation in the presence of the NAC molecule among S.aureus (n= 38) and S.epidermidis (n= 12) isolates isolated from various clinical specimens in Trakya University Health Research and Application Center. In this study, microplate crystal violet method was used to demonstrate the biofilm f...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülcan Kuyucuklu Fatma Kaynak Onurda ğ Canan Ery ıldız Source Type: research

Investigating of Relation Between Fibromyalgia Syndrome and Intestinal Microbiota
In this study, it was aimed to investigate the variation of intestinal microbiome levels in patients with FMS compared to healthy controls. For the investigation of the microbiome, fecal samples were collected from a cohort of 54 patients with FMS and 36 healthy individuals. Those with any mental and/or physical illness in the control group were excluded from the study. The FMS patient group was determined according to the "American College of Rheumatology (ACR)" 2010 diagnostic criteria. The fecal samples were stored at -80°C until use and were thawed on ice; for each extraction, 0.3 g of faeces were weighed...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: B üşra Albayrak Seda S üsgün Okan K üçükakkaş Fahri Akba ş Ay şegül Yabacı Semra Özçelik Source Type: research

Assessment of COVID-19 Risky Contact of Healthcare Workers in an University Hospital
In this study, it was aimed to determine the risk assessment and the factors associated with risk status of an university hospital healthcare workers after risky COVID-19 contact. The data of the descriptive study were obtained from 773 follow-up records of 555 healthcare workers who applied with COVID-19 risky contact between 06.04-10.05.2020. Employees who were positive for RT-PCR evaluated as "patients" and others as "risky contact". Risk assessment was classified as no risk, low, medium and high risk contact according to the "Algorithm of Assessment of Health Workers with COVID-19 Contact"...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülçin Yapıcı Ahmet Öner Kurt Elif Tu ğçe Solmaz Aylin Yeniocak Tun ç Fatma Bozda ğ Beng ü Nehir Buğdaycı Yalçın Ekin Kaplan Burcu Ecem U ğuz Eray D ülger H ülya Özkayalar G önül Aslan G ülden Ersöz Source Type: research

Evaluation of Efficacy of Ribavirin on Laboratory Test and Severity Score in Crimean-Congo Hemorrhagic Fever in Children
Mikrobiyol Bul. 2021 Apr;55(2):180-193. doi: 10.5578/mb.20219905.ABSTRACTCrimean-Congo hemorrhagic fever (CCHF) is endemic in Tokat province. It is observed in all age groups. The case fatality rate varies 20-30% in hospitalized patients and 0-5% among children. Ribavirin use and supportive measures are the main treatment modalities, but the effects of ribavirin in CCHF are controversial. Most of the studies investigating the effectiveness of ribavirin are done by comparing the initial and the final laboratory values. The purpose of this study was to evaluate the effects of oral ribavirin use in children with CCHF by using...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Erhan Karaaslan Şirin Çetin Source Type: research

Evaluation of Healthcare Professionals in Contact with Patients Diagnosed with COVID-19 Detected Outside of the Pandemic Service in our Hospital
In this study, it was aimed to evaluate the contact and illness of healthcare workers working outside of the pandemic clinics in our hospital and to examine the possible transmission routes and disease prognoses. Healthcare workers who were working outside the pandemic service between March 11, 2020 and September 1, 2020 and who had a definite diagnosis of COVID-19 and all hospital staff who had contacted with these people, and HCW who had contact with patients diagnosed with COVID-19 while receiving inpatient treatment in services other than the pandemic service was evaluated and classified as low, medium and high risk ac...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Derya Korkmaz Petek Konya Havva T ünay Ne şe Demirtürk Source Type: research

Evaluation of the Diagnostic Performance of Different Principles of SARS-CoV-2 Commercial Antibody Tests in COVID-19 Patients
Mikrobiyol Bul. 2021 Apr;55(2):207-222. doi: 10.5578/mb.20219907.ABSTRACTFollowing the emergence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) and using only PCR for diagnosis, antibody tests have been rapidly developed by various commercial companies. There are differences between the sensitivity and specificity of these tests due to the usage of different viral target proteins and antibody subclasses. In order to evaluate the diagnostic use of these tests, we aimed to examine the diagnostic performance, especially sensitivity and specificity, of SARS-CoV-2 IgM, IgA and IgG tests of various companies (Ab...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Harika Öykü Dinç Yusuf Emre Özdemir Sena Alkan Zeynep G üngördü Dalar Nesrin Gareayaghi Serhat Sirekbasan Ye şim Tuyji Tok Mert Ahmet Ku şkucu R ıdvan Karaali Do ğukan Özbey G ökhan Aygün Kenan Midilli Bekir Kocazeybek Source Type: research

SARS-CoV-2 Seroprevalence Among Healthcare Workers: Retrospective Analysis of the Data From A University Hospital in Turkey
Mikrobiyol Bul. 2021 Apr;55(2):223-232. doi: 10.5578/mb.20219908.ABSTRACTCOVID-19 infection caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to affect people as a global threat, and the number of cases is increasing every day. Healthcare workers who face potential COVID-19 exposure are at high risk of SARS-CoV-2 transmission. Estimating the prevalence of infection among healthcare professionals, determining the related risk factors and applying effective infection control measures are essential for the continuity of the health system. The aim of this study was to investigate the seroprevalen...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Adem Özdemir Mervenur Demir Çuha G ülçin Telli Dizman Alpaslan Alp G ökhan Metan Bur çin Şener Source Type: research

Seroprevalence of Toxoplasmosis among 20 Years and Older Individuals in Trabzon, Turkey
Mikrobiyol Bul. 2021 Apr;55(2):233-247. doi: 10.5578/mb.20219909.NO ABSTRACTPMID:33882654 | DOI:10.5578/mb.20219909 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Serdar Karakulluk çu Naz ım Ercüment Beyhun Ne şe Kaklıkkaya İftihar Köksal Murat Topba ş Celal Kurtulu ş Buruk Gamze Çan Mustafa Y ılmaz K öksal Hamzaoğlu Esin Say ın Cevriye Ceyda Kolayl ı Source Type: research

Evaluation of the Effect of Hydatid Cyst Fluid on the Apoptosis Pathway in BEAS-2B and A549 Cell Lines
In this study, it was found that the application of hydatid cyst fluid did not directly cause cell death but it has shown for the first time to sensitize the A549 cell line, which is resistant to apoptosisand shed light on the possible mechanism of hydatid cyst fluid in the apoptotic pathway.PMID:33882655 | DOI:10.5578/mb.20219910 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: İpek Baysal Serra Örsten Source Type: research

The Importance of Mycological Diagnosis: A Scedosporium apiospermum Complex Mycetoma Case Neglected For 20 Years
In this report, we aimed to present a case of mycetoma caused by Scedosporium apiospermum complex that developed in a 40-year-old female patient with immunocompetent system and diagnosed by fungal culture. In the anamnesis of the patient who admitted in 2015 with the complaint of more than one fistulized discharge wound, pain and swelling in the dorsal of the right hand and wrist; it was learned that her complaints started about 20 years ago with a slight swelling on the back of the wrist, and when it worsened, the abscess was drained and antibiotic treatment was initiated in a private surgical center. However, it was lear...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ay şe Barış Ahsen Öncül Kahraman Öztürk Alican Bar ış Serkan Aykut Elif Akta ş Source Type: research

New Hopes in Vaccine Technology: mRNA Vaccines
Mikrobiyol Bul. 2021 Apr;55(2):265-284. doi: 10.5578/mb.20219912.ABSTRACTThe major disadvantages of traditional virus vaccines are time constraints in development and difficulties in large-scale production. Therefore, there is a need to develop stronger and more versatile vaccine platforms. mRNA vaccines constitute a promising alternative to traditional vaccine approaches due to their high potential, rapid development capacity, low cost production and safe administration potential. Stability and translation of mRNA are crucial for a successful RNA vaccine. It is critical to determine mRNA purity, stability, and protein yie...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Engin Y ılmaz Source Type: research

Specific Tests and Inflammatory Biomarkers in the Evaluation of Brucellosis Disease
In this study, it was aimed to determine the relationship between laboratory parameters and clinical response and organ involvement in patients with Brucellosis diagnosed with specific diagnostic tests. In the study, 100 patients without previous diagnosis of Brucellosis who have admitted to the Department of Infectious Diseases and Clinical Microbiology and had positive Brucella tube agglutination tests and whose clinic was compatible with Brucellosis were evaluated prospectively. Patients were invited to be checked in the 1st, 2nd and 6th weeks . Patients with hip pain and low back pain were evaluated with sacroiliac mag...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ferhan Kerget Bu ğra Kerget Neslihan Çelik Sibal İba Yılmaz Source Type: research

Effect of N-acetylcystein on Antibiofilm Efficiency of Antibiotics in Staphylococci Isolates
In this study, it was aimed to demonstrate i) the biofilm-forming abilities ii) the change in ampicillin and vancomycin MIC values in the presence of NAC molecules, iii) the change in the MBEK values of these antibiotics in the presence of NAC molecule and iv) the change in the expression levels of genes thought to be related to biofilm formation in the presence of the NAC molecule among S.aureus (n= 38) and S.epidermidis (n= 12) isolates isolated from various clinical specimens in Trakya University Health Research and Application Center. In this study, microplate crystal violet method was used to demonstrate the biofilm f...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülcan Kuyucuklu Fatma Kaynak Onurda ğ Canan Ery ıldız Source Type: research

Investigating of Relation Between Fibromyalgia Syndrome and Intestinal Microbiota
In this study, it was aimed to investigate the variation of intestinal microbiome levels in patients with FMS compared to healthy controls. For the investigation of the microbiome, fecal samples were collected from a cohort of 54 patients with FMS and 36 healthy individuals. Those with any mental and/or physical illness in the control group were excluded from the study. The FMS patient group was determined according to the "American College of Rheumatology (ACR)" 2010 diagnostic criteria. The fecal samples were stored at -80°C until use and were thawed on ice; for each extraction, 0.3 g of faeces were weighed...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: B üşra Albayrak Seda S üsgün Okan K üçükakkaş Fahri Akba ş Ay şegül Yabacı Semra Özçelik Source Type: research

Assessment of COVID-19 Risky Contact of Healthcare Workers in an University Hospital
In this study, it was aimed to determine the risk assessment and the factors associated with risk status of an university hospital healthcare workers after risky COVID-19 contact. The data of the descriptive study were obtained from 773 follow-up records of 555 healthcare workers who applied with COVID-19 risky contact between 06.04-10.05.2020. Employees who were positive for RT-PCR evaluated as "patients" and others as "risky contact". Risk assessment was classified as no risk, low, medium and high risk contact according to the "Algorithm of Assessment of Health Workers with COVID-19 Contact"...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülçin Yapıcı Ahmet Öner Kurt Elif Tu ğçe Solmaz Aylin Yeniocak Tun ç Fatma Bozda ğ Beng ü Nehir Buğdaycı Yalçın Ekin Kaplan Burcu Ecem U ğuz Eray D ülger H ülya Özkayalar G önül Aslan G ülden Ersöz Source Type: research

Evaluation of Efficacy of Ribavirin on Laboratory Test and Severity Score in Crimean-Congo Hemorrhagic Fever in Children
Mikrobiyol Bul. 2021 Apr;55(2):180-193. doi: 10.5578/mb.20219905.ABSTRACTCrimean-Congo hemorrhagic fever (CCHF) is endemic in Tokat province. It is observed in all age groups. The case fatality rate varies 20-30% in hospitalized patients and 0-5% among children. Ribavirin use and supportive measures are the main treatment modalities, but the effects of ribavirin in CCHF are controversial. Most of the studies investigating the effectiveness of ribavirin are done by comparing the initial and the final laboratory values. The purpose of this study was to evaluate the effects of oral ribavirin use in children with CCHF by using...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Erhan Karaaslan Şirin Çetin Source Type: research

Evaluation of Healthcare Professionals in Contact with Patients Diagnosed with COVID-19 Detected Outside of the Pandemic Service in our Hospital
In this study, it was aimed to evaluate the contact and illness of healthcare workers working outside of the pandemic clinics in our hospital and to examine the possible transmission routes and disease prognoses. Healthcare workers who were working outside the pandemic service between March 11, 2020 and September 1, 2020 and who had a definite diagnosis of COVID-19 and all hospital staff who had contacted with these people, and HCW who had contact with patients diagnosed with COVID-19 while receiving inpatient treatment in services other than the pandemic service was evaluated and classified as low, medium and high risk ac...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Derya Korkmaz Petek Konya Havva T ünay Ne şe Demirtürk Source Type: research

Evaluation of the Diagnostic Performance of Different Principles of SARS-CoV-2 Commercial Antibody Tests in COVID-19 Patients
Mikrobiyol Bul. 2021 Apr;55(2):207-222. doi: 10.5578/mb.20219907.ABSTRACTFollowing the emergence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) and using only PCR for diagnosis, antibody tests have been rapidly developed by various commercial companies. There are differences between the sensitivity and specificity of these tests due to the usage of different viral target proteins and antibody subclasses. In order to evaluate the diagnostic use of these tests, we aimed to examine the diagnostic performance, especially sensitivity and specificity, of SARS-CoV-2 IgM, IgA and IgG tests of various companies (Ab...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Harika Öykü Dinç Yusuf Emre Özdemir Sena Alkan Zeynep G üngördü Dalar Nesrin Gareayaghi Serhat Sirekbasan Ye şim Tuyji Tok Mert Ahmet Ku şkucu R ıdvan Karaali Do ğukan Özbey G ökhan Aygün Kenan Midilli Bekir Kocazeybek Source Type: research

SARS-CoV-2 Seroprevalence Among Healthcare Workers: Retrospective Analysis of the Data From A University Hospital in Turkey
Mikrobiyol Bul. 2021 Apr;55(2):223-232. doi: 10.5578/mb.20219908.ABSTRACTCOVID-19 infection caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to affect people as a global threat, and the number of cases is increasing every day. Healthcare workers who face potential COVID-19 exposure are at high risk of SARS-CoV-2 transmission. Estimating the prevalence of infection among healthcare professionals, determining the related risk factors and applying effective infection control measures are essential for the continuity of the health system. The aim of this study was to investigate the seroprevalen...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Adem Özdemir Mervenur Demir Çuha G ülçin Telli Dizman Alpaslan Alp G ökhan Metan Bur çin Şener Source Type: research

Seroprevalence of Toxoplasmosis among 20 Years and Older Individuals in Trabzon, Turkey
Mikrobiyol Bul. 2021 Apr;55(2):233-247. doi: 10.5578/mb.20219909.NO ABSTRACTPMID:33882654 | DOI:10.5578/mb.20219909 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Serdar Karakulluk çu Naz ım Ercüment Beyhun Ne şe Kaklıkkaya İftihar Köksal Murat Topba ş Celal Kurtulu ş Buruk Gamze Çan Mustafa Y ılmaz K öksal Hamzaoğlu Esin Say ın Cevriye Ceyda Kolayl ı Source Type: research

Evaluation of the Effect of Hydatid Cyst Fluid on the Apoptosis Pathway in BEAS-2B and A549 Cell Lines
In this study, it was found that the application of hydatid cyst fluid did not directly cause cell death but it has shown for the first time to sensitize the A549 cell line, which is resistant to apoptosisand shed light on the possible mechanism of hydatid cyst fluid in the apoptotic pathway.PMID:33882655 | DOI:10.5578/mb.20219910 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: İpek Baysal Serra Örsten Source Type: research

The Importance of Mycological Diagnosis: A Scedosporium apiospermum Complex Mycetoma Case Neglected For 20 Years
In this report, we aimed to present a case of mycetoma caused by Scedosporium apiospermum complex that developed in a 40-year-old female patient with immunocompetent system and diagnosed by fungal culture. In the anamnesis of the patient who admitted in 2015 with the complaint of more than one fistulized discharge wound, pain and swelling in the dorsal of the right hand and wrist; it was learned that her complaints started about 20 years ago with a slight swelling on the back of the wrist, and when it worsened, the abscess was drained and antibiotic treatment was initiated in a private surgical center. However, it was lear...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ay şe Barış Ahsen Öncül Kahraman Öztürk Alican Bar ış Serkan Aykut Elif Akta ş Source Type: research

New Hopes in Vaccine Technology: mRNA Vaccines
Mikrobiyol Bul. 2021 Apr;55(2):265-284. doi: 10.5578/mb.20219912.ABSTRACTThe major disadvantages of traditional virus vaccines are time constraints in development and difficulties in large-scale production. Therefore, there is a need to develop stronger and more versatile vaccine platforms. mRNA vaccines constitute a promising alternative to traditional vaccine approaches due to their high potential, rapid development capacity, low cost production and safe administration potential. Stability and translation of mRNA are crucial for a successful RNA vaccine. It is critical to determine mRNA purity, stability, and protein yie...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Engin Y ılmaz Source Type: research

Specific Tests and Inflammatory Biomarkers in the Evaluation of Brucellosis Disease
In this study, it was aimed to determine the relationship between laboratory parameters and clinical response and organ involvement in patients with Brucellosis diagnosed with specific diagnostic tests. In the study, 100 patients without previous diagnosis of Brucellosis who have admitted to the Department of Infectious Diseases and Clinical Microbiology and had positive Brucella tube agglutination tests and whose clinic was compatible with Brucellosis were evaluated prospectively. Patients were invited to be checked in the 1st, 2nd and 6th weeks . Patients with hip pain and low back pain were evaluated with sacroiliac mag...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ferhan Kerget Bu ğra Kerget Neslihan Çelik Sibal İba Yılmaz Source Type: research

Effect of N-acetylcystein on Antibiofilm Efficiency of Antibiotics in Staphylococci Isolates
In this study, it was aimed to demonstrate i) the biofilm-forming abilities ii) the change in ampicillin and vancomycin MIC values in the presence of NAC molecules, iii) the change in the MBEK values of these antibiotics in the presence of NAC molecule and iv) the change in the expression levels of genes thought to be related to biofilm formation in the presence of the NAC molecule among S.aureus (n= 38) and S.epidermidis (n= 12) isolates isolated from various clinical specimens in Trakya University Health Research and Application Center. In this study, microplate crystal violet method was used to demonstrate the biofilm f...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülcan Kuyucuklu Fatma Kaynak Onurda ğ Canan Ery ıldız Source Type: research

Investigating of Relation Between Fibromyalgia Syndrome and Intestinal Microbiota
In this study, it was aimed to investigate the variation of intestinal microbiome levels in patients with FMS compared to healthy controls. For the investigation of the microbiome, fecal samples were collected from a cohort of 54 patients with FMS and 36 healthy individuals. Those with any mental and/or physical illness in the control group were excluded from the study. The FMS patient group was determined according to the "American College of Rheumatology (ACR)" 2010 diagnostic criteria. The fecal samples were stored at -80°C until use and were thawed on ice; for each extraction, 0.3 g of faeces were weighed...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: B üşra Albayrak Seda S üsgün Okan K üçükakkaş Fahri Akba ş Ay şegül Yabacı Semra Özçelik Source Type: research

Assessment of COVID-19 Risky Contact of Healthcare Workers in an University Hospital
In this study, it was aimed to determine the risk assessment and the factors associated with risk status of an university hospital healthcare workers after risky COVID-19 contact. The data of the descriptive study were obtained from 773 follow-up records of 555 healthcare workers who applied with COVID-19 risky contact between 06.04-10.05.2020. Employees who were positive for RT-PCR evaluated as "patients" and others as "risky contact". Risk assessment was classified as no risk, low, medium and high risk contact according to the "Algorithm of Assessment of Health Workers with COVID-19 Contact"...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülçin Yapıcı Ahmet Öner Kurt Elif Tu ğçe Solmaz Aylin Yeniocak Tun ç Fatma Bozda ğ Beng ü Nehir Buğdaycı Yalçın Ekin Kaplan Burcu Ecem U ğuz Eray D ülger H ülya Özkayalar G önül Aslan G ülden Ersöz Source Type: research

Evaluation of Efficacy of Ribavirin on Laboratory Test and Severity Score in Crimean-Congo Hemorrhagic Fever in Children
Mikrobiyol Bul. 2021 Apr;55(2):180-193. doi: 10.5578/mb.20219905.ABSTRACTCrimean-Congo hemorrhagic fever (CCHF) is endemic in Tokat province. It is observed in all age groups. The case fatality rate varies 20-30% in hospitalized patients and 0-5% among children. Ribavirin use and supportive measures are the main treatment modalities, but the effects of ribavirin in CCHF are controversial. Most of the studies investigating the effectiveness of ribavirin are done by comparing the initial and the final laboratory values. The purpose of this study was to evaluate the effects of oral ribavirin use in children with CCHF by using...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Erhan Karaaslan Şirin Çetin Source Type: research

Evaluation of Healthcare Professionals in Contact with Patients Diagnosed with COVID-19 Detected Outside of the Pandemic Service in our Hospital
In this study, it was aimed to evaluate the contact and illness of healthcare workers working outside of the pandemic clinics in our hospital and to examine the possible transmission routes and disease prognoses. Healthcare workers who were working outside the pandemic service between March 11, 2020 and September 1, 2020 and who had a definite diagnosis of COVID-19 and all hospital staff who had contacted with these people, and HCW who had contact with patients diagnosed with COVID-19 while receiving inpatient treatment in services other than the pandemic service was evaluated and classified as low, medium and high risk ac...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Derya Korkmaz Petek Konya Havva T ünay Ne şe Demirtürk Source Type: research

Evaluation of the Diagnostic Performance of Different Principles of SARS-CoV-2 Commercial Antibody Tests in COVID-19 Patients
Mikrobiyol Bul. 2021 Apr;55(2):207-222. doi: 10.5578/mb.20219907.ABSTRACTFollowing the emergence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) and using only PCR for diagnosis, antibody tests have been rapidly developed by various commercial companies. There are differences between the sensitivity and specificity of these tests due to the usage of different viral target proteins and antibody subclasses. In order to evaluate the diagnostic use of these tests, we aimed to examine the diagnostic performance, especially sensitivity and specificity, of SARS-CoV-2 IgM, IgA and IgG tests of various companies (Ab...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Harika Öykü Dinç Yusuf Emre Özdemir Sena Alkan Zeynep G üngördü Dalar Nesrin Gareayaghi Serhat Sirekbasan Ye şim Tuyji Tok Mert Ahmet Ku şkucu R ıdvan Karaali Do ğukan Özbey G ökhan Aygün Kenan Midilli Bekir Kocazeybek Source Type: research

SARS-CoV-2 Seroprevalence Among Healthcare Workers: Retrospective Analysis of the Data From A University Hospital in Turkey
Mikrobiyol Bul. 2021 Apr;55(2):223-232. doi: 10.5578/mb.20219908.ABSTRACTCOVID-19 infection caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to affect people as a global threat, and the number of cases is increasing every day. Healthcare workers who face potential COVID-19 exposure are at high risk of SARS-CoV-2 transmission. Estimating the prevalence of infection among healthcare professionals, determining the related risk factors and applying effective infection control measures are essential for the continuity of the health system. The aim of this study was to investigate the seroprevalen...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Adem Özdemir Mervenur Demir Çuha G ülçin Telli Dizman Alpaslan Alp G ökhan Metan Bur çin Şener Source Type: research

Seroprevalence of Toxoplasmosis among 20 Years and Older Individuals in Trabzon, Turkey
Mikrobiyol Bul. 2021 Apr;55(2):233-247. doi: 10.5578/mb.20219909.NO ABSTRACTPMID:33882654 | DOI:10.5578/mb.20219909 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Serdar Karakulluk çu Naz ım Ercüment Beyhun Ne şe Kaklıkkaya İftihar Köksal Murat Topba ş Celal Kurtulu ş Buruk Gamze Çan Mustafa Y ılmaz K öksal Hamzaoğlu Esin Say ın Cevriye Ceyda Kolayl ı Source Type: research

Evaluation of the Effect of Hydatid Cyst Fluid on the Apoptosis Pathway in BEAS-2B and A549 Cell Lines
In this study, it was found that the application of hydatid cyst fluid did not directly cause cell death but it has shown for the first time to sensitize the A549 cell line, which is resistant to apoptosisand shed light on the possible mechanism of hydatid cyst fluid in the apoptotic pathway.PMID:33882655 | DOI:10.5578/mb.20219910 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: İpek Baysal Serra Örsten Source Type: research

The Importance of Mycological Diagnosis: A Scedosporium apiospermum Complex Mycetoma Case Neglected For 20 Years
In this report, we aimed to present a case of mycetoma caused by Scedosporium apiospermum complex that developed in a 40-year-old female patient with immunocompetent system and diagnosed by fungal culture. In the anamnesis of the patient who admitted in 2015 with the complaint of more than one fistulized discharge wound, pain and swelling in the dorsal of the right hand and wrist; it was learned that her complaints started about 20 years ago with a slight swelling on the back of the wrist, and when it worsened, the abscess was drained and antibiotic treatment was initiated in a private surgical center. However, it was lear...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ay şe Barış Ahsen Öncül Kahraman Öztürk Alican Bar ış Serkan Aykut Elif Akta ş Source Type: research

New Hopes in Vaccine Technology: mRNA Vaccines
Mikrobiyol Bul. 2021 Apr;55(2):265-284. doi: 10.5578/mb.20219912.ABSTRACTThe major disadvantages of traditional virus vaccines are time constraints in development and difficulties in large-scale production. Therefore, there is a need to develop stronger and more versatile vaccine platforms. mRNA vaccines constitute a promising alternative to traditional vaccine approaches due to their high potential, rapid development capacity, low cost production and safe administration potential. Stability and translation of mRNA are crucial for a successful RNA vaccine. It is critical to determine mRNA purity, stability, and protein yie...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Engin Y ılmaz Source Type: research

Specific Tests and Inflammatory Biomarkers in the Evaluation of Brucellosis Disease
In this study, it was aimed to determine the relationship between laboratory parameters and clinical response and organ involvement in patients with Brucellosis diagnosed with specific diagnostic tests. In the study, 100 patients without previous diagnosis of Brucellosis who have admitted to the Department of Infectious Diseases and Clinical Microbiology and had positive Brucella tube agglutination tests and whose clinic was compatible with Brucellosis were evaluated prospectively. Patients were invited to be checked in the 1st, 2nd and 6th weeks . Patients with hip pain and low back pain were evaluated with sacroiliac mag...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ferhan Kerget Bu ğra Kerget Neslihan Çelik Sibal İba Yılmaz Source Type: research

Effect of N-acetylcystein on Antibiofilm Efficiency of Antibiotics in Staphylococci Isolates
In this study, it was aimed to demonstrate i) the biofilm-forming abilities ii) the change in ampicillin and vancomycin MIC values in the presence of NAC molecules, iii) the change in the MBEK values of these antibiotics in the presence of NAC molecule and iv) the change in the expression levels of genes thought to be related to biofilm formation in the presence of the NAC molecule among S.aureus (n= 38) and S.epidermidis (n= 12) isolates isolated from various clinical specimens in Trakya University Health Research and Application Center. In this study, microplate crystal violet method was used to demonstrate the biofilm f...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülcan Kuyucuklu Fatma Kaynak Onurda ğ Canan Ery ıldız Source Type: research

Investigating of Relation Between Fibromyalgia Syndrome and Intestinal Microbiota
In this study, it was aimed to investigate the variation of intestinal microbiome levels in patients with FMS compared to healthy controls. For the investigation of the microbiome, fecal samples were collected from a cohort of 54 patients with FMS and 36 healthy individuals. Those with any mental and/or physical illness in the control group were excluded from the study. The FMS patient group was determined according to the "American College of Rheumatology (ACR)" 2010 diagnostic criteria. The fecal samples were stored at -80°C until use and were thawed on ice; for each extraction, 0.3 g of faeces were weighed...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: B üşra Albayrak Seda S üsgün Okan K üçükakkaş Fahri Akba ş Ay şegül Yabacı Semra Özçelik Source Type: research

Assessment of COVID-19 Risky Contact of Healthcare Workers in an University Hospital
In this study, it was aimed to determine the risk assessment and the factors associated with risk status of an university hospital healthcare workers after risky COVID-19 contact. The data of the descriptive study were obtained from 773 follow-up records of 555 healthcare workers who applied with COVID-19 risky contact between 06.04-10.05.2020. Employees who were positive for RT-PCR evaluated as "patients" and others as "risky contact". Risk assessment was classified as no risk, low, medium and high risk contact according to the "Algorithm of Assessment of Health Workers with COVID-19 Contact"...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülçin Yapıcı Ahmet Öner Kurt Elif Tu ğçe Solmaz Aylin Yeniocak Tun ç Fatma Bozda ğ Beng ü Nehir Buğdaycı Yalçın Ekin Kaplan Burcu Ecem U ğuz Eray D ülger H ülya Özkayalar G önül Aslan G ülden Ersöz Source Type: research

Evaluation of Efficacy of Ribavirin on Laboratory Test and Severity Score in Crimean-Congo Hemorrhagic Fever in Children
Mikrobiyol Bul. 2021 Apr;55(2):180-193. doi: 10.5578/mb.20219905.ABSTRACTCrimean-Congo hemorrhagic fever (CCHF) is endemic in Tokat province. It is observed in all age groups. The case fatality rate varies 20-30% in hospitalized patients and 0-5% among children. Ribavirin use and supportive measures are the main treatment modalities, but the effects of ribavirin in CCHF are controversial. Most of the studies investigating the effectiveness of ribavirin are done by comparing the initial and the final laboratory values. The purpose of this study was to evaluate the effects of oral ribavirin use in children with CCHF by using...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Erhan Karaaslan Şirin Çetin Source Type: research

Evaluation of Healthcare Professionals in Contact with Patients Diagnosed with COVID-19 Detected Outside of the Pandemic Service in our Hospital
In this study, it was aimed to evaluate the contact and illness of healthcare workers working outside of the pandemic clinics in our hospital and to examine the possible transmission routes and disease prognoses. Healthcare workers who were working outside the pandemic service between March 11, 2020 and September 1, 2020 and who had a definite diagnosis of COVID-19 and all hospital staff who had contacted with these people, and HCW who had contact with patients diagnosed with COVID-19 while receiving inpatient treatment in services other than the pandemic service was evaluated and classified as low, medium and high risk ac...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Derya Korkmaz Petek Konya Havva T ünay Ne şe Demirtürk Source Type: research

Evaluation of the Diagnostic Performance of Different Principles of SARS-CoV-2 Commercial Antibody Tests in COVID-19 Patients
Mikrobiyol Bul. 2021 Apr;55(2):207-222. doi: 10.5578/mb.20219907.ABSTRACTFollowing the emergence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) and using only PCR for diagnosis, antibody tests have been rapidly developed by various commercial companies. There are differences between the sensitivity and specificity of these tests due to the usage of different viral target proteins and antibody subclasses. In order to evaluate the diagnostic use of these tests, we aimed to examine the diagnostic performance, especially sensitivity and specificity, of SARS-CoV-2 IgM, IgA and IgG tests of various companies (Ab...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Harika Öykü Dinç Yusuf Emre Özdemir Sena Alkan Zeynep G üngördü Dalar Nesrin Gareayaghi Serhat Sirekbasan Ye şim Tuyji Tok Mert Ahmet Ku şkucu R ıdvan Karaali Do ğukan Özbey G ökhan Aygün Kenan Midilli Bekir Kocazeybek Source Type: research

SARS-CoV-2 Seroprevalence Among Healthcare Workers: Retrospective Analysis of the Data From A University Hospital in Turkey
Mikrobiyol Bul. 2021 Apr;55(2):223-232. doi: 10.5578/mb.20219908.ABSTRACTCOVID-19 infection caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to affect people as a global threat, and the number of cases is increasing every day. Healthcare workers who face potential COVID-19 exposure are at high risk of SARS-CoV-2 transmission. Estimating the prevalence of infection among healthcare professionals, determining the related risk factors and applying effective infection control measures are essential for the continuity of the health system. The aim of this study was to investigate the seroprevalen...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Adem Özdemir Mervenur Demir Çuha G ülçin Telli Dizman Alpaslan Alp G ökhan Metan Bur çin Şener Source Type: research

Seroprevalence of Toxoplasmosis among 20 Years and Older Individuals in Trabzon, Turkey
Mikrobiyol Bul. 2021 Apr;55(2):233-247. doi: 10.5578/mb.20219909.NO ABSTRACTPMID:33882654 | DOI:10.5578/mb.20219909 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Serdar Karakulluk çu Naz ım Ercüment Beyhun Ne şe Kaklıkkaya İftihar Köksal Murat Topba ş Celal Kurtulu ş Buruk Gamze Çan Mustafa Y ılmaz K öksal Hamzaoğlu Esin Say ın Cevriye Ceyda Kolayl ı Source Type: research

Evaluation of the Effect of Hydatid Cyst Fluid on the Apoptosis Pathway in BEAS-2B and A549 Cell Lines
In this study, it was found that the application of hydatid cyst fluid did not directly cause cell death but it has shown for the first time to sensitize the A549 cell line, which is resistant to apoptosisand shed light on the possible mechanism of hydatid cyst fluid in the apoptotic pathway.PMID:33882655 | DOI:10.5578/mb.20219910 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: İpek Baysal Serra Örsten Source Type: research

The Importance of Mycological Diagnosis: A Scedosporium apiospermum Complex Mycetoma Case Neglected For 20 Years
In this report, we aimed to present a case of mycetoma caused by Scedosporium apiospermum complex that developed in a 40-year-old female patient with immunocompetent system and diagnosed by fungal culture. In the anamnesis of the patient who admitted in 2015 with the complaint of more than one fistulized discharge wound, pain and swelling in the dorsal of the right hand and wrist; it was learned that her complaints started about 20 years ago with a slight swelling on the back of the wrist, and when it worsened, the abscess was drained and antibiotic treatment was initiated in a private surgical center. However, it was lear...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ay şe Barış Ahsen Öncül Kahraman Öztürk Alican Bar ış Serkan Aykut Elif Akta ş Source Type: research

New Hopes in Vaccine Technology: mRNA Vaccines
Mikrobiyol Bul. 2021 Apr;55(2):265-284. doi: 10.5578/mb.20219912.ABSTRACTThe major disadvantages of traditional virus vaccines are time constraints in development and difficulties in large-scale production. Therefore, there is a need to develop stronger and more versatile vaccine platforms. mRNA vaccines constitute a promising alternative to traditional vaccine approaches due to their high potential, rapid development capacity, low cost production and safe administration potential. Stability and translation of mRNA are crucial for a successful RNA vaccine. It is critical to determine mRNA purity, stability, and protein yie...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Engin Y ılmaz Source Type: research

Specific Tests and Inflammatory Biomarkers in the Evaluation of Brucellosis Disease
In this study, it was aimed to determine the relationship between laboratory parameters and clinical response and organ involvement in patients with Brucellosis diagnosed with specific diagnostic tests. In the study, 100 patients without previous diagnosis of Brucellosis who have admitted to the Department of Infectious Diseases and Clinical Microbiology and had positive Brucella tube agglutination tests and whose clinic was compatible with Brucellosis were evaluated prospectively. Patients were invited to be checked in the 1st, 2nd and 6th weeks . Patients with hip pain and low back pain were evaluated with sacroiliac mag...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: Ferhan Kerget Bu ğra Kerget Neslihan Çelik Sibal İba Yılmaz Source Type: research

Effect of N-acetylcystein on Antibiofilm Efficiency of Antibiotics in Staphylococci Isolates
In this study, it was aimed to demonstrate i) the biofilm-forming abilities ii) the change in ampicillin and vancomycin MIC values in the presence of NAC molecules, iii) the change in the MBEK values of these antibiotics in the presence of NAC molecule and iv) the change in the expression levels of genes thought to be related to biofilm formation in the presence of the NAC molecule among S.aureus (n= 38) and S.epidermidis (n= 12) isolates isolated from various clinical specimens in Trakya University Health Research and Application Center. In this study, microplate crystal violet method was used to demonstrate the biofilm f...
Source: Mikrobiyoloji Bulteni - April 22, 2021 Category: Microbiology Authors: G ülcan Kuyucuklu Fatma Kaynak Onurda ğ Canan Ery ıldız Source Type: research