[Direct Identification of Microorganisms from Positive Blood Cultures by MALDI-TOF MS Using an in-House Tween ® 80 Method: Experimental and Clinical Study].
In this study, it was aimed to develop a simple, easy-to-apply and reliable protocol for identifying the agent directly from the blood culture bottle that gives positive signal with the use of detergent Tween® 80, and to study the obtained protocol in clinical samples in a routine microbiology laboratory and to evaluate the results. The study was carried out in two stages, the experimental stage where the method was developed and the clinical stage where the method was applied. In the experimental stage, blood culture bottles were created with standard strains and isolates previously diagnosed with the 16S rRNA method....
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[The Identification of Acinetobacter baumannii Blood Isolates by MALDI-TOF MS, ARDRA and blaOXA-51-like Gene-Specific Real-Time PCR].
In this study, we aimed to evaluate the diagnosis performance of MALDI-TOF MS system to identify blood isolates of A.baumannii. A total of 180 nonduplicate carbapenem resistant Acb complex (strain numbers; TR1-TR60) and A.baumannii (TR61-TR180) blood isolates were collected from the intensive care units of the three university hospitals in Turkey from January 2016 to December 2016. All isolates were evaluated by using blaOXA-51-like gene specific real time (Rt-PCR) analysis, ARDRA (restriction enzymes-AluI, CfoI, MboI, MspI, RsaI) method and MALDI-TOF MS (VITEK® MS, bioMérieux, France) system. All the strains ex...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Pseudomonas aeruginosa Biofilm Formation and Quorum Sensing Genes in Piperacillin/Tazobactam and Ciprofloxacin Sub-minimal Inhibitory Concentrations].
Authors: Erdal B, Yalınay M, Elmas Ç, Yazıcı GN Abstract Pseudomonas aeruginosa is a non-fermentative, oxidase-positive, motile gram-negative bacillus widespread in nature. The virulence factors of P.aeruginosa including the ability to grow under minimal growth conditions, the widespread presence in nature, and the ability to form biofilms make P.aeruginosa a highly important bacterium along with its resistance mechanisms against many antibiotics. The ability to form biofilms increases the symptom severity in diseases caused by P.aeruginosa and causes difficulties in the treatment. The aim of this study w...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of Mycobacterium tuberculosis Culture and Drug Susceptibility Test Results and the Distribution of Nontuberculosis Mycobacteria from the Clinical Specimens].
In this study, it was aimed to retrospectively evaluate the epidemiological status of the Mycobacterium [Mycobacterium tuberculosis complex (MTC) and NTM] obtained from the clinical specimens of patients with TB suspicion, and the resistance rates of MTC isolates against anti-TB drugs. Various clinical samples of TB suspected patients sent to the Balıkesir Atatürk City Hospital Mycobacteriology Laboratory between 2011 and 2019, were included in the study. Microscopy, culture procedures, and the first-line anti-TB drug susceptibility tests were performed according to the instructions. Identification of NTM at the spec...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of Patients Diagnosed with COVID-19 in Terms of Risk Factors].
In this study, we aimed to investigate the demographic characteristics and risk factors of patients with the diagnosis of COVID-19. A total of 100 patients (53 female and 47 male) were included in our study. The patients included in the study were randomly selected from the registration system and their information was evaluated retrospectively. The mean age of the patients was 54.42 (Age range= 20-90). When the risk factors for catching the disease were evaluated; it was determined that there was at least one risk factor in 46 patients; 30 patients had close contact with the COVID-19 patient in the social environment (30%...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Pneumocystis jirovecii Infection and Colonization in Immunocompromised Patients with Pneumonia].
In this study, it was aimed to investigate PCP and colonizations by microscopy, polymerase chain reaction (PCR) and Krebs von den Lungen-6 (KL-6) tests in symptomatic immunosuppressive inpatients with the sign of radiologically atypical pneumonia in Mersin University Hospital. A total of 96 patients, between August 2016 and February 2018 were included in the study. Seventy two (75%) of the 96 patients were under immunosuppressive therapy. P.jirovecii was investigated in the respiratory tract samples [sputum (n= 88), tracheal aspirate (n= 6) and bronchoalveolar lavage (n= 2)] by mtLSUrRNA nested PCR and microscopic staining...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of a Commercial Multiplex Tandem Polymerase Chain Reaction Method for the Identification of the Yeasts Isolated from Blood Cultures].
In this study, we aimed to evaluate the Fungal ID MT-PCR test (AusDiagnostics, Australia) for the identification of the yeasts from positive blood cultures in Akdeniz University Hospital Central Laboratory. Between December 2016 and December 2017, blood culture samples from 92 consecutive patients with yeast cells detected in Gram stained smears were tested by Fungal ID MT-PCR and the reference method. After the subculture of the positive signaling blood culture bottles to Sabouraud dextroz agar (SDA), the identification of the yeasts were performed by morphological identification methods (Germ tube test, Corn Meal Tween&r...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of the Two Commercial Methods Based on Broth Microdilution Against Reference Microdilution Method for Klebsiella pneumoniae Isolates].
Authors: Kansak N, Aslan M, Adaleti R, Aksaray S Abstract A rapid and reliable method for antimicrobial susceptibility test of colistin is needed because of increasing numbers of multi-resistant gram negative bacterial infections and simultaneus increasing of colistin resistance. Although broth microdilution (BMD) is recommended by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee for Antimicrobial Susceptibility Testing (EUCAST) as a reference method, the use in routine laboratory practice is limited because of the difficulties in application and time-consuming characteristics. Rece...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Comparison of Two Anti-HIV Confirmatory Assays: Recombinant HIV 1/2 Line Immunoassay and Geenius HIV 1/2 Confirmatory Test].
In this study, the supplemental recombinant HIV 1/2 LIA (INNO-LIA®, Fujirebio, Ghent, Belgium) used to confirm and differentiate the diagnosis of HIV-1 and HIV-2 infections, and an immunochromatographic supplemental test (Geenius™ (Bio-Rad Laboratories, Marnes-la-Coquette, France) which can provide faster results were compared. One hundred fifty serum samples sent to Ege University Faculty of Medicine Hospital Medical Virology Laboratory with anti-HIV 1/2 and p24 antigen positive and indeterminant results and three HIV-1 positive external quality control samples were included in the study. Samples were tested bot...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Ganciclovir Resistance in Cytomegalovirus Strains Obtained from Immunocompromised Patients].
Authors: Coşkun A, Gökahmetoğlu S, Özmen P, Çevik Ş, Karakükçü M, Kaynar L, Midilli K, Kuşkucu MA Abstract CMV is a virus that is asymptomatic in healthy individuals but can cause serious mortality and morbidity in transplant patients and patients with acquired immunodeficiency syndrome (AIDS). Ganciclovir (GCV) is a nucleoside analog that significantly reduces morbidity and mortality in CMV-related infections and is used as the first choice in treatment. It is the first drug shown to be effective in the treatment of CMV disease in humans, and is also homologous to acyclovir. L...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of SARS-CoV-2 PCR Positive Cases in the Pediatric Emergency Department].
In this study, it was aimed to evaluate the epidemiological, clinical, laboratory and radiologic findings, treatment and clinical outcomes of patients admitted to the pediatric emergency department with the suspicion of COVID-19. Between March 11 and June 16, 2020, patients aged between 1 month-18 years admitted to the pediatric emergency department and who have an indication for sampling for the polymerase chain reaction (PCR) method with the suspicion of COVID-19 according to the current guidelines published by the Ministry of Health were included in the study. The demographic characteristics, symptoms, durations and the...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Distribution of Yeast Species Isolated from Blood Cultures for a Six Year Period in Turkey: a Multicentre Study].
Authors: Ergon MC, Doluca Dereli M, Ener B, Atalay MA, Koç AN, Çerikçioğlu N, Erturan Z, Aksaray S Abstract Bloodstream infections due to yeast species especially Candida spp. have been reported to be important healthcare associated infections with high mortality and morbidity rates. Candidemia causes prolonged hospital stays as well as increased cost. In order to prevent or treat these life-threatening bloodstream infections successfully, nationwide epidemiological data should be available about the etiological agents of these infections. Multi-centre national epidemiological data on yeast bl...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Cutaneous Leishmaniasis Cases Caused by Leishmania infantum in Şanlıurfa Province, Turkey].
In this study, we aimed to present the first CL cases caused by Leishmania infantum in Şanlıurfa. A total of 14 cases, which were admitted with the suspicion of CL and diagnosed as positive by direct microscopy and/or real-time ITS1-PCR using lesion aspiration samples are included in the study. Two or more smears were prepared from the samples taken from the lesions of the patients by fine needle aspiration. One of the smears was stained with Giemsa stain after fixation with methyl alcohol and examined under the light microscope at x1000 magnification for the presence of Leishmania amastigotes. DNA isolation was made fro...
Source: Mikrobiyoloji Bulteni - October 28, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Employee Safety and Biosafety Applications in Microbiology Laboratories; A Multicenter Evaluation in Turkey].
Authors: Köroğlu M, Altındiş S, Aydemir Ö, Yüksel B, Demiray T, Erkorkmaz Ü, Aslan FG, Otlu B, Altındiş M, LabBioSafety TR Çalışma Grubu Abstract Medical laboratory personnel may be exposed to various hazards, especially biological and chemical, during their routine activities. In this multicenter study, which could reflect the nation wide results, it was aimed to determine the safety and biosecurity practices of the employee working in medical microbiology laboratories and to reveal the current situation. A total of 1072 personnel working in the Medical Microbiology Laboratory of ...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[In Vitro Activities of Antimicrobials Against Toxigenic Clostridioides difficile Isolates Obtained in a University Training and Research Hospital in Turkey].
In conclusion, two main antimicrobial agents commonly used in the treatment of C.difficile infections, metronidazole and vancomycin, seem to be effective. However, high resistance rates against to the certain tested antimicrobials highlight the need for further surveillance to monitor the emergence of resistance. PMID: 32755514 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Klebsiella pneumoniae Infections in the Intensive Care Unit: Risk Factors Related to Carbapenem Resistance and Patient Mortality].
Authors: Büyüktuna SA, Hasbek M, Çelik C, Ünlüsavuran M, Avcı O, Baltacı S, Fırtına Topcu K, Elaldı N Abstract Klebsiella pneumoniae is the cause of complicated and difficult-to-treat nosocomial infections such as sepsis, urinary tract infection, catheter related infections, pneumonia and surgical site infections in intensive care units. The biggest problem in infections with K.pneumoniae is that treatment options are limited due to multiple antibiotic resistance and consequently the increased morbidity and mortality. The widespread and improper use of carbapenems can lead to epidemi...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Molecular Characterization of Lucimycin Gene of Lucilia sericata].
In this study, continuous production of adult colonies of L.sericata species was carried out in insectarium unit where conditions such as light, humidity and temperature were formed. The life cycle of L.sericata was followed and the production of eggs, larvae, pupae, adult flies and fly colonies of the species were formed. In the third stage larvae obtained from adult flies in the insectarium unit, RNA was isolated and subsequently cDNA synthesis was performed by reverse transcription. Polymerase chain reaction (PCR) analysis of the synthesized cDNAs with the specific primers designed for the lucimycin gene of L.sericata w...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Antimicrobial Susceptibility of Pathogenic Gram-positive Anaerobic Cocci: Data of a University Hospital in Turkey].
This study is one of the largest susceptibility studies specifically carried out on GPAC to date in Turkey. We believe that our results will provide good surveillance data both for our hospital and our country. PMID: 32755517 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of Real-Time Polymerase Chain Reaction in Pneumocystis jirovecii Laboratory Diagnosis].
In this study, we aimed to evaluate the value of real-time PCR (Rt-PCR) in the laboratory diagnosis of P.jirovecii. Bronchoalveolar lavage (BAL) specimens of 658 patients sent to Dokuz Eylul University Hospital Central Medical Parasitology Laboratory on suspicion of PCP were included in the study. BAL fluids were evaluated for identification of P.jirovecii mitochondrial gene coding ribosomal large subunit (mtLSUrRNA) using Rt-PCR. In addition, Giemsa and Gomori's methenamine silver (GMG) staining assays were applied to all samples and nested PCR (n-PCR) assay was applied to positive samples detected by real time PCR. Ninet...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Diversity of Leishmania Strains Isolated from Cutaneous Leishmaniasis Patients in Turkey and its Reflection to Clinics in Mice Model].
Authors: Özbilgin A, Çulha G, Güray MZ, Zeyrek FY, Akyar I, Töz S, Östan Ural İ, Kurt Ö, Kocagöz T, Çavuş İ, Gündüz C Abstract Although asexual reproduction has been attributed to Leishmania species, genetic exchange has recently been demonstrated, which helped emerging of hybrid isolates. Situated on the crossroads between three continents, Leishmania hybrids may be present in Turkey. In Turkey, visceral leishmaniasis caused by Leishmania infantum is less common, while cutaneous leishmaniasis (CL) caused by Leishmania tropica and L.infantum could reach 2500 ...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Determination of Antimony Resistance Mechanism of Leishmania tropica Causing Cutaneous Leishmaniasis in Turkey].
In conclusion, it was revealed that L.tropica strains in our country may gain resistance to meglumine antimoniate in a short time. It is foreseen that if the patients living in our country or entering the country are treated inadequately and incompletely, there may be new, resistant leishmaniasis foci that may increase the number of resistant strains and cases rapidly. PMID: 32755520 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[An Endemic Plant of Cyprus, Origanum majorana: Is It A New Alternative Natural Product for Malaria Treatment?]
Authors: Güler E, Özbilgin A, Becer E, Hanoğlu A, Şanlıdağ T Abstract Malaria still remains to be a public health threat and one of the most important infectious diseases to get attention from World Health Organization. No domestic malaria cases have been reported on the island of Cyprus since 1948, as a result of successful elimination process. All of the malaria cases detected in recent years are imported cases. As known, hundreds of medicines are obtained from plants and traditional medicine are used in endemic places of malaria. The cause of malaria - Plasmodium parasites, are developing resistanc...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Anti-leishmanial Effects of Bee Products (Honey, Propolis) on Leishmania tropica Promastigotes].
This study was aimed to investigate the anti-leishmanial effects of bee products (honey and propolis) by using the causative agent of cutaneous leishmaniasis Leishmania tropica promastigotes, in in vitro culture. In vitro anti-leishmanial efficacy of honey (pine, flower and chestnut) and propolis used in the study were evaluated using the microdilution method. Honey, which is a bee product, was dissolved with RPMI medium containing fetal calf serum (FCS) and diluted in the same medium, and serial dilutions were prepared in concentrations between 62.5-1000 mg/ml. Propolis, on the other hand, was dissolved with ethyl alcohol...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Urogenital Myiasis Caused by Psychoda spp. in Female Patient with No Risk Factor for Myiasis].
In conclusion, many regions are suitable for Psychoda type of adult flies to live in our country due to the climate conditions. It is considered that infestation may develop in patients with less risk factors in terms of urogenital myiasis, that larvae may increase in number of patients who are not treated or followed up and that the infestation may become chronic and results in more serious clinical conditions. The necessary treatment and follow-up must be performed in such cases and the patients should be educated about the relationship between infestation and hygiene. PMID: 32755523 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[SARS-CoV-2 and Microbiological Diagnostic Dynamics in COVID-19 Pandemic].
Authors: Erensoy S Abstract We have been introduced to "Coronavirus Disease 2019 (COVID-19)" disease with high mortality and transmission rate caused by a novel human coronavirus, in December 2019 and the microbiological diagnosis of the infection has been in the center of the focus to control the pandemic. It is necessary to understand the dynamics of the virus which was classified among the severe acute respiratory syndrome (SARS) related coronaviruses and named as SARS coronavirus 2 (SARS-CoV-2), to manage testing in the right strategy and for interpretation of the results. However, much remains unclea...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Cystic Echinococcosis in Humans in Turkey].
Authors: Ok ÜZ, Kilimcioğlu AA, Özkol M Abstract Although cystic echinococcosis (CE) is quite prevalent in Turkey, it is extremely neglected due to being usually asymptomatic for years and frequently not to be reported although it is obligatory. Most of the data on the prevalence of CE in humans in Turkey are based on hospital records, reported cases and the studies based on serological methods and they do not reflect the truth. The fact that detecting no cysts in most of the seropositive cases limits the value of researches based on serological tests. The most valuable epidemiologic data on CE are obtai...
Source: Mikrobiyoloji Bulteni - August 13, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Plasmid Mediated mcr Colistin Resistance Gene in Clinical Enterobacterales Isolates].
In conclusion, as far as we can reach in the literature our publication is the first study showing the presence of mcr-1 gene in clinical samples in our country and confirmed by DNA sequence analysis. The detection of mcr gene in isolates without multidrug resistance showed once again the importance of colistin susceptibility testing in the laboratories. In addition, the presence of isolates containing more than one resistance genes in our study, suggests that the spread of carbapenem and colistin resistance may be faster than expected. PMID: 32723275 [PubMed - as supplied by publisher] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Tularemia Outbreak and After; Effect of Seasonal Changes].
This study included tularemia cases seen in Yozgat and its districts between 2010 and 2016. Tularemia was defined as a microagglutination test (MAT) result of ≥ 1/160 or a 4-fold increase in MAT titer between two tests at least two weeks apart, in the presence of consistent clinical findings. Seasonal factors were recorded. The conformity of data to normal distribution was analyzed using the ShapiroWilk test. The Mann-Whitney U test was used with the results of Monte Carlo simulations to compare differences between two independent groups in terms of quantitative data. It was found that tularemia cases are more frequentl...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Using of the Sensititre MycoTB Plate Method for the Detection of Anti-tuberculous Drug Susceptibility of Rifampin Resistant Mycobacterium tuberculosis Complex Isolates].
In this study, it was aimed to evaluate whether the Sensititre MycoTB plaque method is suitable for the routine use in determining drug susceptibility of rifampin resistant MTBC strains. Xpert MTBC/ RIF positive rifampin resistant 100 MTBC isolates were included in the study. Xpert MTBC/RIF (Cepheid, USA) test were performed after the samples were processed by homogenization and decontamination and acid-fast staining. Rifampin resistant clinical samples were cultured in automated MGIT/BACTEC 960 (Becton Dickinson, USA) system and acid fast bacteria (AFB) were investigated. The anti-TB drug susceptibility tests of all cultu...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Determination of Biofilm Formation Properties of Methicillin Sensitive and Resistant Staphylococcus aureus Isolates by Conventional and Molecular Methods].
In conclusion, determining the potential of biofilm formation of colonizing or causative agents and taking essential precautions before interventional procedures will decrease biofilm related infections and related morbidity and mortality. PMID: 32723278 [PubMed - as supplied by publisher] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[The Microbiological Analysis of a Rhizobium radiobacter Outbreak After Intravitreal Injection].
This study is the first epidemic outbreak report of endophthalmitis caused by the same strain of R.radiobacter and the second article in which R.radiobacter was reported as the cause of endophthalmitis after intravitreal injection. PMID: 32723279 [PubMed - as supplied by publisher] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Antimicrobial Susceptibility Profiles of Prevotella Species Determined by Gradient Test Method in Two Centers in Istanbul].
In conclusion, piperacillin/tazobactam, cefoxitin, and tigecycline displayed high in vitro activity against Prevotella spp. and they all remained good candidates for empiric therapy. Imipenem and meropenem were also found to be very active, but the usage of carbapenems should be reserved for serious mixed infections, potentially accompanied by other resistant organisms. Intermediate resistance to ampicillinsulbactam and the resistance against metronidazole emphasized the need of periodic monitoring of their susceptibility patterns. The high rates of non-susceptibility to ampicillin, clindamycin, tetracycline and moxifloxac...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Comparison of Two Commercial Quantitative Cytomegalovirus (CMV) Polymerase Chain Reaction Tests Calibrated by World Health Organization International CMV Standard].
In this study, it was aimed to compare the results of the two commercial World Health Organization (WHO) International CMV standard calibrated polymerase chain reaction tests, CMV Cobas Ampliprep/Cobas Taqman (CMV-CAP/CTM) (Roche, Germany) and Artus CMV QIASymphony-Rotorgene (CMV-QS-RGQ) (Qiagen, Germany). Both tests were performed simultaneously on 244 plasma samples. The results were measured in copies/ml and converted to IU/ml by multiplying with 0.91 for CMV-CAP/CTM and 1.64 for CMV-QS-RGQ, as specified by the manufacturers. CMV DNA was detected in 174 (71.3%) and was not detected in 52 (21.3%) of the samples and eight...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Regulatory T Cells and Secreted Immunomodulatory Cytokine IL-10 Levels in Patients with Hepatitis B].
In this study, we aimed to determine whether evaluation of Treg cells and cytokine IL-10 levels together in hepatitis B patients is useful that may indicate the disease survey and response to the treatment. The peripheral blood samples of ninety-one volunteers, including 61 HBV infected patients and 30 healthy controls selected from applicants of Infectious Diseases Outpatient/Clinic Service, were taken. Their CD4+CD25highFOXP3+CD152+CD127lowTreg cell distribution were measured by flow cytometry method, using the recently defined markers. The level of IL-10 cytokine released by immunomodulatory cells was determined by quan...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of the Relationship Between TNF- α, IL-12A, IL-12B and IFN-γ Gene Polymorphisms and Chronic Hepatitis C].
[Investigation of the Relationship Between TNF-α, IL-12A, IL-12B and IFN-γ Gene Polymorphisms and Chronic Hepatitis C]. Mikrobiyol Bul. 2020 Apr;54(2):279-290 Authors: Börekçi G, Çetinkaya A, Kandemir Ö, Bekalp Yılmaz İ, Orekici Temel G, Aras N Abstract Hepatitis C virus (HCV) infection is still an important public health problem worldwide. Cytokines play an important role in the prognosis of HCV infections. Polymorphisms in the cytokine genes can affect the gene expression and change the clinical course of the disease. The aim of this study was to determine the re...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Phenotypic and Genotypic Evaluation of Azole Resistance in Aspergillus fumigatus Isolates from Clinical and Environmental Specimens].
Authors: Doğan Ö, Gülmez D, Arıkan Akdağlı S Abstract Aspergillus fumigatus can cause different clinical manifestations including chronic pulmonary infections, as well as invasive aspergillosis which is highly mortal in the immunocompromised host. Azole antifungal drugs, voriconazole in particular, are the first-line recommended therapeutic regimen. Azoles inhibit 14-α demethylase enzyme encoded by the cyp51A gene. In recent years, increased azole resistance is observed among environmental and clinical A.fumigatus isolates. Two different mechanisms have been proposed for the development of resis...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Comparison of Multiplex Nested PCR, In-house Real-Time PCR and Commercial Real-Time PCR Methods for the Detection of Plasmodium spp. from Blood Samples].
In this study, it was aimed to compare the results of three different molecular methods and determine which molecular method could be used in the diagnostic algorithm to be applied. DNA was extracted from 280 whole blood sample stored in EDTA tubes using a commercial kit. Three different polymerase chain reaction (PCR) methods were used for the detection of Plasmodium spp. in DNA samples obtained and the results were compared. First, multiplex nested PCR was applied and then in-house real-time PCR (Rt-PCR) which was validated in our laboratory and a commercial Rt-PCR kit were applied. Multiplex nested PCR was accepted as t...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Frequency of Influenza, Influenza Types and Influenza Vaccine Use in Hospitalized Children with Influenza-Like Symptoms].
Authors: Erat T, Özdemir H, Taşkınoğlu T, İnce E, Çiftçi E Abstract Influenza is an important cause of respiratory illness in children and is still an important cause of morbidity and mortality in children. The influenza virus subtypes determine the prevalence of the epidemic and pandemic influenza, the hospitalization and mortality rates in children each year. Surveillance of the circulation of different influenza virus strains is important in ensuring a good strain con-cordance for the composition of the annual influenza vaccine. The Global Influenza Hospital Surveillance Network® (GIHS...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[A Case of Simultaneous Acute Lymphoblastic Leukemia Diagnosis with Crimean-Congo Hemorrhagic Fever].
In this study, we present a case of ALL who was diagnosed with CCHF simultaneously. A 43-year old female patient who works in the library and does not have a chronic disease other than asthma and thyroid disorder, has admitted to our hospital with the complaints of intermittent fever, weakness, generalized joint and body pain for about 3 weeks. She had fever and the physical examination revealed bilateral cervical and right postauricular lymphadenopathies. Her aspartate aminotransferase: 77 U/L, alanine aminotransferase: 117 U/L, lactate dehydrogenase: 616 U/L, hemoglobin: 8.27 g/dl, leukocyte count: 15.690/mm3 , neutrophi...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Candida hellenica Candidemia Occurred After Esophagus Surgery].
In this study, a case of candidemia caused by Candida hellenica as the first report in our country was presented. Fluconazole and liposomal amphotericin B treatment was initiated in a 20-year-old male patient in October 2018 due to the diagnosis of candidemia following esophageal surgery. The patient had a history of multiple esophageal operations. The patient was discharged during the last 24 hours due to the lack of fever, improvement in general condition and lack of growth in blood cultures. Germination tube test of the Candida isolate grown in blood culture was negative and the colony morphology in corn meal tween 80 a...
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Visceral Leishmaniasis and Hemophagocytic Lymphohistiocytosis in an Adult Patient].
Conclusions: PMID: 32723289 [PubMed - as supplied by publisher] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - July 30, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Determination of the Subspecies of Francisella tularensis Isolated in Turkey by Molecular Methods].
Authors: Çelebi B, Bakkaloğlu Z, Ünaldı Ö, Karagöz A, Kılıç S, Durmaz R Abstract Francisella tularensis is a gram-negative, coccobasillus, facultative intracellular bacteria and causes a zoonotic disease, tularemia in humans. F.tularensis has four subspecies, which have different virulences for humans as F.tularensis subsp. tularensis, F.tularensis subsp. holarctica, F.tularensis subsp. mediasiatica and F.tularensis subsp. novicida. F.tularensis subsp. tularensis is the most virulent subspecies and mortality rate is high in human cases. F.tularensis subsp. holarctica, which has been...
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Virulence Genes, Antibiotic Susceptibility Profiles and Molecular Characterization of Campylobacter Species Isolated from Different Slaughterhouses].
Authors: Hızlısoy H, Al S, Ertaş Onmaz N, Yıldırım Y, Gönülalan Z, Barel M, Güngör C, Dışhan A, Dişli HB Abstract The aim of this study was to investigate the frequency of Campylobacter species, to detect the antibiotic resistance profiles and the virulence genes and to determine the clonal proximity of the isolates in the samples of cutting board, slaughterhouse waste water, wall, knife and carcass from three different slaughterhouses in Kayseri region. For this purpose, a total of 150 samples, 10 of each from knife, wall, cutting board, carcass smear sample and slaughterhouse wastewat...
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Antibiotic Resistance and Virulence Factors of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Clinical Samples].
In conclusion, our data indicated that virulence factors except hyl gene and biofilm production were higher in E.faecalis isolates but E.faecium isolates were more resistant to antibiotics. In order to prevent infection of such virulent or resistant isolates in the hospital setting, infection control measures must be followed. In vivo studies are needed for the better understanding of the virulence of enterococci. PMID: 32050876 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Existence of Biofilm and Biofilm-Associated Virulence Genes in Multi-Drug Resistant Invasive Acinetobacter baumannii Isolates].
In this study, when the distribution of virulence genes were compAred with the isolates that have weak, medium and strong biofilm, all of the studied genes were found to be more abundant in isolates with strong and medium positive biofilm production. This has shown that excluding fimH gene, csgA, csuE and ompA genes have contributed to the biofilm formation in invasive A.baumannii isolates, respectively. PMID: 32050877 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of the Incidence of Legionella and Free-Living Amoebae in Swimming Pool Waters and Biofilm Specimens in Istanbul by Different Methods].
This study is the first study to investigate the presence of Legionella and FLA in swimming pools in Istanbul, and further studies are needed to examine more pool water and biofilm samples. With the data obtained, the health principles and controls of swimming pools will be re-considered and will be contributed to public health. PMID: 32050878 [PubMed - in process] (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Comparison of mRNA Levels of Stimulator of Interferon Genes (STING) in Individuals with Natural Immunity to Hepatitis B Virus (HBV), and in those with Chronic Hepatitis B Infection and without HBV].
Authors: Kasap B, Buruk CK, Kaklıkkaya N, Özkaya E, Aydın Kasap Z, Aydın F Abstract It has been estimated that currently 350-400 million people have been chronically infected with the hepatitis B virus (HBV) worldwide and approximately one million people die each year due to HBV related diseases. It has been suggested that the viral and host factors, especially the host immune system, may play a role in the chronicity of the HBV infection. Stimulator of interferon genes (STING) is one of the members of the pattern recognition receptor (PRR) that detects the presence of DNA in a human cell, activate synthesi...
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Comparison of Antiviral Effect of Olive Leaf Extract and Propolis with Acyclovir on Herpes Simplex Virus Type 1].
Authors: Altındiş M, Aslan FG, Uzuner H, Ünal H, Köroğlu M, Kulaç S, Karadenizli A Abstract While acyclovir, a nucleoside analogue, is widely used for herpes simplex virus type 1 (HSV-1), emergence of drug-resistant viruses due to frequent usage of this class of medicines, and their toxic side effects require exploring novel active molecules. Despite the studies on developing synthetic molecules in medical sciences and pharmacology, herbs as a natural source of biologically-active compounds remain popular. In this in vitro study, olive leaf extract (OLE) and propolis alone or in combination with...
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Evaluation of Histological Response in Chronic Hepatitis B Patients with Tenofovir or Entecavir Therapy].
Authors: Çerçioğlu D, Kınıklı S, Cesur S, Ataman Hatipoğlu Ç, Arslan K, Gönültaş M Abstract Chronic hepatitis B (CHB) is an important public health problem in the world and Turkey. The aim of this study was to evaluate the histological, virological, serological and biochemical response rates in CHB patients receiving tenofovir or entecavir therapy. Control liver biopsies were performed on patients who received tenofovir or entecavir therapy for one year or longer. Histopathological grading was scored according to the modified Knodell system. Eighty-seven CHB patients were includ...
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

[Investigation of Anti-HCV S/CO Value in Detecting Viremia in Patients with Hepatitis C Virus Infection].
In this study, it was aimed to obtain an optimal anti-HCV S/CO value in our laboratory for demonstrating true antibody positivity and viremia in patients by analyzing the relationship between anti-HCV, alanine aminotransferase (ALT) and HCV RNA using retrospective data. Between July 2014 and July 2017, 754.190 anti-HCV tests were performed. Patients aged 18 years or older who were reactive with anti-HCV and those with simultaneous HCV RNA and ALT prompts were included in the study. The second generation CMIA (Abbott, USA) method was used for anti-HCV detection. For quantitative HCV RNA analysis, viral nucleic acid extracti...
Source: Mikrobiyoloji Bulteni - February 14, 2020 Category: Microbiology Tags: Mikrobiyol Bul Source Type: research