Development of a topical treatment for tegumentary leishmaniasis using 8-hydroxyquinoline
Eur J Pharm Biopharm. 2024 Apr 26:114306. doi: 10.1016/j.ejpb.2024.114306. Online ahead of print.ABSTRACTIn the context of neglected diseases, tegumentary leishmaniasis (TL) presents an emerging and re-emerging character in the national territory and in the world. The treatment of TL has limitations, such as intravenous administration route, high toxicity, and high treatment costs. Thus, several researchers work on new therapeutic strategies to improve the effectiveness of the treatment of leishmaniasis. In this light, the present study used a topical formulation, containing 8-hydroquinoline (8-HQN), for the treatment of B...
Source: European Journal of Pharmaceutics and Biopharmaceutics - April 28, 2024 Category: Drugs & Pharmacology Authors: Naianda Rezende Ribeiro Lucas Magno Oliveira Santos Scarleth Silva Costa Carolina Kei Myasaki Daniela Pagliara Lage Vivian Tamietti Martins Rory Cristiane Fortes De Brito Larissa Chaves Freire Ana Maria Ravena Severino Carvalho Bryan Victor Serafim Brigid Source Type: research
The cuticle proteome of a planktonic crustacean
AbstractThe cuticles of arthropods provide an interface between the organism and its environment. Thus, the cuticle's structure influences how the organism responds to and interacts with its surroundings. Here, we used label-free quantification proteomics to provide a proteome of the moulted cuticle of the aquatic crustaceanDaphnia magna, which has long been a prominent subject of studies on ecology, evolution, and developmental biology. We detected a total of 278 high-confidence proteins. Using protein sequence domain and functional enrichment analyses, we identified chitin-binding structural proteins and chitin-modifying...
Source: Proteomics - April 28, 2024 Category: Biochemistry Authors: Kathrin A. Otte,
Maridel Fredericksen,
Peter Fields,
Thomas Fr öhlich,
Christian Laforsch,
Dieter Ebert Tags: DATASET BRIEF Source Type: research
Impact of water quality and morphometric indices on the spatio-temporal prevalence of fish endo-parasites and diversity in the Ase River, Niger-Delta, Nigeria
AbstractThree stations along the Ase River, Delta State Nigeria provided water and 85 fish samples which were analyzed. The fish were measured and examined for endoparasites according to established protocol. All water quality parameters investigated were within the WHO-acceptable values for surface waters. Station variation of physicochemical parameters was not statistically significant (p > 0.05). Fish body conformation indices positively correlated with the prevalence of parasites inClarias gariepinus, Heterobranchus longifilis, Parachana africana, Chromidotilapia guntheri guntherii, andDenticeps clupeodes. The ...
Source: Journal of Parasitic Diseases - April 28, 2024 Category: Parasitology Source Type: research
A hybrid approach based on multipath Swin transformer and ConvMixer for white blood cells classification
AbstractWhite blood cells (WBC) play an effective role in the body ’s defense against parasites, viruses, and bacteria in the human body. Also, WBCs are categorized based on their morphological structures into various subgroups. The number of these WBC types in the blood of non-diseased and diseased people is different. Thus, the study of WBC classification is qu ite significant for medical diagnosis. Due to the widespread use of deep learning in medical image analysis in recent years, it has also been used in WBC classification. Moreover, the ConvMixer and Swin transformer models, recently introduced, have garnered sign...
Source: Health Information Science and Systems - April 28, 2024 Category: Information Technology Source Type: research
Molecules, Vol. 29, Pages 2034: Effects of Central Metal Ion on Binuclear Metal Phthalocyanine-Based Redox Mediator for Lithium Carbonate Decomposition
Haiyong He
Li2CO3 is the most tenacious parasitic solid-state product in lithium–air batteries (LABs). Developing suitable redox mediators (RMs) is an efficient way to address the Li2CO3 issue, but only a few RMs have been investigated to date, and their mechanism of action also remains elusive. Herein, we investigate the effects of the central metal ion in binuclear metal phthalocyanines on the catalysis of Li2CO3 decomposition, namely binuclear cobalt phthalocyanine (bi-CoPc) and binuclear cobalt manganese phthalocyanine (bi-CoMnPc). Density functional theory (DFT) calculations indicate that the key ...
Source: Molecules - April 28, 2024 Category: Chemistry Authors: Qinghui Yan Linghui Yan Haoshen Huang Zhengfei Chen Zixuan Liu Shaodong Zhou Haiyong He Tags: Article Source Type: research
Ghosts of parasites past influence current non-consumptive effects in Drosophila nigrospiracula
Int J Parasitol. 2024 Apr 25:S0020-7519(24)00078-X. doi: 10.1016/j.ijpara.2024.04.008. Online ahead of print.ABSTRACTParasites can indirectly impact hosts through non-consumptive effects (NCEs) via changes in behaviour, morphology, and/or physiology. These responses can be understood in terms of the ecology of fear (ectoparasites) or the ecology of disgust (endoparasites) framework. We tested the hypothesis that NCEs of parasite exposure (e.g., parasite avoidance and defense) trade off with other important behaviours such as feeding and resting. We predicted that when exposed to parasites (without infection), hosts will in...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: C Liang L T Luong Source Type: research
Novel CRISPR-Cas-powered pen-side test for East Coast fever
We describe a streamlined, field-applicable diagnostic tool comprising a 20 min recombinase polymerase amplification (RPA) reaction followed by a 60 min CRISPR-Cas12a reaction using a FAM/Biotin lateral flow strip readout. We tested two different RPA primer pairs and four different CRISPR-RNAs (crRNAs). The p104-based assay displayed high sensitivity, detecting as low as one infected lymphocyte per three microliters of blood and universally detecting eight different T. parva strains without detecting DNA from other Theileria spp. such as Theileria mutans and Theileria lestoquardi. This work opens the way for a field-applic...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: Robert Muriuki Maingi Ndichu Samuel Githigia Nicholas Svitek Source Type: research
Optimization of benzenesulfonyl derivatives as anti-Trypanosomatidae agents: Structural design, synthesis, and pharmacological assessment against Trypanosoma cruzi and Leishmania infantum
This study details the early stages of hit-to-lead optimization for a benzenesulfonyl derivative, denoted as initial hit, against Trypanossoma cruzi (T. cruzi), Leishmania infantum (L. infantum) and Leishmania braziliensis (L. braziliensis). We investigated structure - activity relationships using a series of 26 newly designed derivatives, ultimately yielding potential lead candidates with potent low-micromolar and sub-micromolar activities against T. cruzi and Leishmania spp, respectively, and low in vitro cytotoxicity against mammalian cells. These discoveries emphasize the significant promise of this chemical class in t...
Source: Bioorganic and Medicinal Chemistry - April 27, 2024 Category: Chemistry Authors: Guilherme Freitas de Lima Hercos Mariza Gabriela Faleiro de Moura Lodi Cruz Ana Clara Cassiano Martinho Daniela de Melo Resende Danilo Farago Nascimento Paula Derksen Macruz Eduardo Jorge Pilau Silvane Maria Fonseca Murta Celso de Oliveira Rezende J únio Source Type: research
Genetic Diversity of Blastocystis in Diarrheal Cases: Identification of Subtypes and Alleles
In conclusion, our study provides important data on the molecular epidemiological characteristics of the Blastocystis by determining positivity, subtypes and alleles in diarrheal cases. Therefore, within the scope of the one health approach, comprehensive molecular epidemiological studies are required to determine the presence and genotypes of Blastocystis in human, animal and environmental samples.PMID:38676586 | DOI:10.5578/mb.202498207 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Evren Tileklio ğlu Hatice Ertabaklar Source Type: research
Hookworm genomics: dusk or dawn?
Trends Parasitol. 2024 Apr 26:S1471-4922(24)00085-0. doi: 10.1016/j.pt.2024.04.003. Online ahead of print.ABSTRACTHookworms are parasites, closely related to the model nematode Caenorhabditis elegans, that are a major economic and health burden worldwide. Primarily three hookworm species (Necator americanus, Ancylostoma duodenale, and Ancylostoma ceylanicum) infect humans. Another 100 hookworm species from 19 genera infect primates, ruminants, and carnivores. Genetic data exist for only seven of these species. Genome sequences are available from only four of these species in two genera, leaving 96 others (particularly thos...
Source: Trends in Parasitology - April 27, 2024 Category: Parasitology Authors: Vladislav Il ík Erich M Schwarz Eva Noskov á Barbora Paf čo Source Type: research
Novel CRISPR-Cas-powered pen-side test for East Coast fever
We describe a streamlined, field-applicable diagnostic tool comprising a 20 min recombinase polymerase amplification (RPA) reaction followed by a 60 min CRISPR-Cas12a reaction using a FAM/Biotin lateral flow strip readout. We tested two different RPA primer pairs and four different CRISPR-RNAs (crRNAs). The p104-based assay displayed high sensitivity, detecting as low as one infected lymphocyte per three microliters of blood and universally detecting eight different T. parva strains without detecting DNA from other Theileria spp. such as Theileria mutans and Theileria lestoquardi. This work opens the way for a field-applic...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: Robert Muriuki Maingi Ndichu Samuel Githigia Nicholas Svitek Source Type: research
Ghosts of parasites past influence current non-consumptive effects in Drosophila nigrospiracula
Int J Parasitol. 2024 Apr 25:S0020-7519(24)00078-X. doi: 10.1016/j.ijpara.2024.04.008. Online ahead of print.ABSTRACTParasites can indirectly impact hosts through non-consumptive effects (NCEs) via changes in behaviour, morphology, and/or physiology. These responses can be understood in terms of the ecology of fear (ectoparasites) or the ecology of disgust (endoparasites) framework. We tested the hypothesis that NCEs of parasite exposure (e.g., parasite avoidance and defense) trade off with other important behaviours such as feeding and resting. We predicted that when exposed to parasites (without infection), hosts will in...
Source: International Journal for Parasitology - April 27, 2024 Category: Parasitology Authors: C Liang L T Luong Source Type: research
Optimization of benzenesulfonyl derivatives as anti-Trypanosomatidae agents: Structural design, synthesis, and pharmacological assessment against Trypanosoma cruzi and Leishmania infantum
This study details the early stages of hit-to-lead optimization for a benzenesulfonyl derivative, denoted as initial hit, against Trypanossoma cruzi (T. cruzi), Leishmania infantum (L. infantum) and Leishmania braziliensis (L. braziliensis). We investigated structure - activity relationships using a series of 26 newly designed derivatives, ultimately yielding potential lead candidates with potent low-micromolar and sub-micromolar activities against T. cruzi and Leishmania spp, respectively, and low in vitro cytotoxicity against mammalian cells. These discoveries emphasize the significant promise of this chemical class in t...
Source: Bioorganic and Medicinal Chemistry - April 27, 2024 Category: Chemistry Authors: Guilherme Freitas de Lima Hercos Mariza Gabriela Faleiro de Moura Lodi Cruz Ana Clara Cassiano Martinho Daniela de Melo Resende Danilo Farago Nascimento Paula Derksen Macruz Eduardo Jorge Pilau Silvane Maria Fonseca Murta Celso de Oliveira Rezende J únio Source Type: research
Genetic Diversity of Blastocystis in Diarrheal Cases: Identification of Subtypes and Alleles
In conclusion, our study provides important data on the molecular epidemiological characteristics of the Blastocystis by determining positivity, subtypes and alleles in diarrheal cases. Therefore, within the scope of the one health approach, comprehensive molecular epidemiological studies are required to determine the presence and genotypes of Blastocystis in human, animal and environmental samples.PMID:38676586 | DOI:10.5578/mb.202498207 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Evren Tileklio ğlu Hatice Ertabaklar Source Type: research
Hookworm genomics: dusk or dawn?
Trends Parasitol. 2024 Apr 26:S1471-4922(24)00085-0. doi: 10.1016/j.pt.2024.04.003. Online ahead of print.ABSTRACTHookworms are parasites, closely related to the model nematode Caenorhabditis elegans, that are a major economic and health burden worldwide. Primarily three hookworm species (Necator americanus, Ancylostoma duodenale, and Ancylostoma ceylanicum) infect humans. Another 100 hookworm species from 19 genera infect primates, ruminants, and carnivores. Genetic data exist for only seven of these species. Genome sequences are available from only four of these species in two genera, leaving 96 others (particularly thos...
Source: Trends in Parasitology - April 27, 2024 Category: Parasitology Authors: Vladislav Il ík Erich M Schwarz Eva Noskov á Barbora Paf čo Source Type: research
