Murine cathelicidin: as a host defensive response against Leishmania major infection
AbstractLeishmaniasis is a serious global challenge with neither efficacious prophylactic vaccine nor effective and safe therapeutic measures. Cathelicidins, members of antimicrobial peptides family, are small proteins of innate immunity system, which represent a protective barrier against a number of potential pathogens in living organisms. The murine cathelicidin or cathelin-related antimicrobial peptide (CRAMP) is expressed by a variety of cells or tissues, and highly resembles to human cathelicidin (LL-37). It is naturally expressed at a low concentration in adolescent age, but extensively increases during cutaneous in...
Source: Journal of Parasitic Diseases - July 4, 2020 Category: Parasitology Source Type: research

High prevalence of intestinal helminthic infection among children under 5  years in a rural Ghanaian community: an urgent call for attention
This study recorded an overall parasitic infections of 44.08%. This therefore calls for periodic screening, anti-helminthic treatment of these children as well as intensified education on attitudinal/behavioral change on improved personal and environmental hygiene in order to help control the menace of intestinal parasitic infections. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - July 4, 2020 Category: Parasitology Source Type: research

Prevalence and risk factors associated with infection by Eimeria spp. in goats and sheep in Northeastern Brazil
AbstractEimeria spp. infections lead to acute or chronic intestinal disorders in small ruminants, being responsible for important economic losses worldwide. The aim of this study was to assess the risk factors associated withEimeria spp. infection in small ruminants of the microregion of Garanhuns, state of Pernambuco, Northeastern Brazil. Fecal samples (n  = 822) were obtained from goats (n = 414) and sheep (n = 408) and evaluated by the modified Gordon and Whitlock technique. Risk factors were assessed through univariate analysis and logistical regression. Oocysts ofEimeria speci...
Source: Journal of Parasitic Diseases - June 26, 2020 Category: Parasitology Source Type: research

Comparison of various methods for DNA extraction from human isolated paraffin-embedded hydatid cyst samples
In this study, we are going to describe a method that can extract DNA from human isolated paraffin-embedded hydatid cysts with a high quality and quantity. Formalin fixed and Paraffin-embedded hydatid cyst samples isolated from human lung and archived in the pathology laboratory were used for this purpose. Several sections of the paraffin blocks were prepared with 5 micron thickness and DNA were extracted by three different methods including; modified boiling, commercial kit and the method described by Larissa A. Pikor et al. The obtained DNA were evaluated by Nanodrop in terms of the yield of DNA and possible contaminatio...
Source: Journal of Parasitic Diseases - June 23, 2020 Category: Parasitology Source Type: research

Ectoparasites of feral horses [ Equus ferus caballus (Linnaeus., 1758)] on Karada ğ Mountain, Karaman, Turkey
This study was carried out to determine the presence of ectopara sites infesting a subsample of 36 feral horses. The horses were visually inspected, and then their bodies were checked by hand for ectoparasites. Thirty-five (97.2%) were infested with at least one of five species of ectoparasites:Bovicola equi (Linnaeus, 1758),Hippobosca equina (Linnaeus, 1758),Haemaphysalis parva (Neuman, 1897),Hyalomma excavatum (Koch, 18449),Dermacentor marginatus (Sulzer, 1776). Most of the horses were coinfested with two ectoparasite species. Prevalence of infestation withH. equina was 80.6% and withB. equi 72.2%. In addition, prevalenc...
Source: Journal of Parasitic Diseases - June 11, 2020 Category: Parasitology Source Type: research

The efficacy and dosage of Mentha piperita essential oil in the control of Monogenean parasites in Oreochromis niloticus
According to this study,M. piperita essential oil demonstrated toxic toO. niloticus fingerlings at concentrations of 40, 45 and 50  mg/L, causing histological changes, changes in the color of the animal and death, and ineffectiveness against the monogeneans parasites after therapeutic bath in the highest concentration of essential oil tolerated by the studied fish specimens. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - June 8, 2020 Category: Parasitology Source Type: research

Apigenin effect against Leishmania tropica amastigotes in vitro
This study aims to evaluate the efficacy of Apigenin against L.tropica amastigotes in vitro using Leishmania-Macrophage Interaction Assay. Our study demonstrated the possibility of efficacy of Apigenin against L.tropica amastigotes since Apigenin reduced the infection index at IC50 60.44 µM and this requires subsequent studies in humans and using Apigenin as a candidate for the chemotherapeutic treatment against Leishmaniasis. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - May 20, 2020 Category: Parasitology Source Type: research

Prevalence of Eimeria species in sheep ( Ovis aries ) from Dakahlia governorate, Egypt
AbstractFeces from 184 sheep from Dakahlia governorate, Egypt were tested forEimeria species oocysts by using the standard floatation technique; oocysts were detected in 126 (68.4%). The prevalence was significantly higher in young sheep than adults. ElevenEimeria species were identified:Eimeria ahsata,Eimeria bakuensis,Eimeria crandallis,Eimeria faurei,Eimeria granulosa,Eimeria intricata,Eimeria marsica,Eimeria ovinoidalis,Eimeria pallida,Eimeria parva and Eimeria webybridgensis. Oocysts of the most pathogenic ovine  species,E. ovinoidalis, were detected in 27 (14.6%) sheep. This is the first report ofE. webybridgens...
Source: Journal of Parasitic Diseases - May 19, 2020 Category: Parasitology Source Type: research

Effect of oxfendazole to control Taenia solium cysticercosis in pigs in Punjab state of India
AbstractTaenia solium cysticercosis is a neglected zoonosis prevalent in developing countries including India. The disease has a substantial health impact in India. The control of this disease in pigs would benefit both veterinary and public health. In view of this, the current study was designed to know the effectiveness of oxfendazole againstT. solium in pigs reared in Punjab, India. A randomized controlled trail was designed with two groups of treatment (T1 and T2) and a control group. Pigs from ten different litters were selected. Pigs (4  months of age) from the same litter were randomly assigned into these three...
Source: Journal of Parasitic Diseases - May 4, 2020 Category: Parasitology Source Type: research

Prevalence of gastrointestinal helminth parasites of trade cattle in Aguata and Orumba South Local Government Areas, Southeastern Nigeria
AbstractThe prevalence of gastrointestinal helminth parasites of trade cattle were investigated in Aguata and Orumba South Local Government Areas (LGA), Southeastern Nigeria. Between February and May, 2017, 210 cattle were randomly sampled in Aguata (140) and Orumba South (70) LGAs. Characteristics of each trade cattle such as sex, breed and body condition scores were noted. Fecal samples were collected per rectum from the selected animals and were subjected to floatation and sedimentation techniques. The overall prevalence of gastrointestinal helminth parasites was 57.6% (95% CI  = 0.509–0.643). Of the...
Source: Journal of Parasitic Diseases - May 4, 2020 Category: Parasitology Source Type: research

Retraction Note to: Antigen based detection of cystic echinococcosis in buffaloes using ELISA and Dot-EIA
The Editor-in-Chief has retracted this article [1] because it has been previously published [2]. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - April 29, 2020 Category: Parasitology Source Type: research

Study on the effect of an ion channel inhibitor “Fluralaner” on Echinococcus granulosus protoscolices and metacestode layers in vitro
AbstractHydatid disease has a great impact on public health, causing high morbidity and mortality. Main lines of treatment include surgery, which mostly requires the installation of a scolicidal agent into hydatid cysts to prevent dissemination. Alternatively, medical treatment involves the use of benzimidazole drugs; however, the results are not satisfactory, and new drug compounds are urgently needed. Fluralaner is a potent inhibitor of GABA-gated chloride channels and L-glutamate-gated chloride channels (GluCls) providing immediate and persistent flea, tick and mite control in dogs after a single oral dose. Researches p...
Source: Journal of Parasitic Diseases - April 24, 2020 Category: Parasitology Source Type: research

Occurrence and molecular identification of Anisakis larval type 1 (Nematoda: Anisakidae) in marketed fish in Egypt
This study highlights the importance of these fish as potential reservoirs for human anisakiasis in Egypt and possibly in other coastal countries. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - April 24, 2020 Category: Parasitology Source Type: research

Prevalence and molecular characterisation of Echinococcus granulosus in disposed of bovine carcasses in Punjab, India
We report 21% (18/85) prevalence of hydatidosis in dispose of bovine carcasses. Age was reported as the significant risk factor (Chi square  = 22.5, df = 2, p = 0) for hydatidosis in the studied population and this might be due to higher cumulative exposure to the environmental parasitic stage (E. granulosus eggs) with increase in age. Phylogenetic analysis showed that G1 (predominantly) and G3 strains ofE. granulosus are circulating in bovine population in Punjab. Further, studies are needed to determine the strains ofE. granulosus circulating in the stray dogs. Measures and polic...
Source: Journal of Parasitic Diseases - April 21, 2020 Category: Parasitology Source Type: research

Comparision of the various routine diagnostic modalities of malaria and a new method: the Parasight ™ platform
AbstractMalaria is one of most important parasitic disease, which is still much prevalent in India. The burden of malaria in India is complex and the proportions of  Plasmodium vivax and Plasmodium falciparum vary across India, because of the highly variable malaria eco-epidemiological profiles, transmission factors, and the presence of multiple Plasmodium species and Anopheles vectors. The diagnostic modalities which were being used currently, are at the risk of missing potential malaria cases, if a single test is being used for a given sample. There are some extremely sensitive and...
Source: Journal of Parasitic Diseases - April 20, 2020 Category: Parasitology Source Type: research

Evaluation of tumor necrosis factor-alpha (TNF- α); gamma interferon (IFN-γ) genes and oxidative stress in sheep: immunological responses induced by Oestrus ovis (Diptera: Oestridae) infestation
This study aimed to evaluate the cell mediated immune responses againstOestrus ovis (O. ovis) in sheep through measurement of the changes in mRNA expression of the tumor necrosis factor alpha (TNF- α) and gamma interferon (IFN-γ) cytokines using quantitative Real time-PCR (qRt-PCR). Also; to detect the role ofOestrus ovis infestation in the oxidative stress markers in sheep. Fifty sheep head were examined in Cairo abattoir from the period of May to August 2019. Sera were separated and collected for measurement of nitric oxide, zinc and malondialdehyde (MDA). While TNF- α and IFN-γ mRNA were extracte...
Source: Journal of Parasitic Diseases - April 15, 2020 Category: Parasitology Source Type: research

Prevalence, seasonal and geographical distribution of parasitic diseases in dogs in Plateau State Nigeria: a 30-year retrospective study (1986 –2015)
AbstractParasitic diseases of dogs are now major economic and public health issues in Nigeria due to indiscriminate reproduction of dogs, lack of appropriate policies on pet ownership and diseases control as well as inadequate veterinary care. In order to understand the prevalence, seasonal variation and distribution of parasitic diseases of dogs in this region of Nigeria, the present study conducted a 30-year retrospective analysis in the referral veterinary centre, Vom, and analysed data using the Chi square test and the factor analysis. Of the 26,844 dogs handled between January 1986 and December 2015, 17,663 (65.8%) ha...
Source: Journal of Parasitic Diseases - April 10, 2020 Category: Parasitology Source Type: research

Molecular detection of Ehrlichia ruminantium in engorged ablyomma variegatum and cattle in Ogun State, Nigeria
AbstractEarly diagnosis ofEhrlichia ruminantium in cattle is a recipe for effective control of heartwater in ruminants. Hence, we assessed the presence ofE. ruminantium in the blood of cattle and the engorgedAmblyomma variegatum by nested PCR. The electrophoresed PCR products obtained after primary and secondary amplifications revealed amplicon sizes of about350 bp and 280  bp respectively, which corresponded with the partial region of pSC20 gene amplified. Sequences obtained had 95–99% homology with those sequences available in GenBank. The prevalence of theE. ruminantium in ticks (50%; 126/252) was significantly (p 
Source: Journal of Parasitic Diseases - April 8, 2020 Category: Parasitology Source Type: research

Otodectes cynotis in urban and peri-urban semi-arid areas: a widespread parasite in the cat population
This study investigates the presence of this parasite in 296 cats from Murcia municipality (SE Spain), and describes possible factors associated with the infestation. Cats were grouped by sex, age, lifestyle, season and provenience. Scraping samples were examined by a microscope to identify the mite. Chi square test was computed and odds ratio was used to measure the association of risk factors with parasite prevalence. Additionally, the spatial distribution of prevalences was investigated and represented through GIS software. Around 30% of the cats (CI95 25 –35%) were found positives toO. cynotis. The mite infestati...
Source: Journal of Parasitic Diseases - March 27, 2020 Category: Parasitology Source Type: research

Diagnosis and therapeutic management of gastrointestinal parasitism among the captive population of Indian star tortoise ( Geochelone elegans Schoepff, 1795) at Zoological Garden, Thiruvananthapuram, Kerala, India
AbstractIndian star tortoise (Geochelone elegans Schoepff, 1795) is a terrestrial herbivorous reptile that is classified under the vulnerable species of IUCN-Red List. The present communication reports the successful management of gastrointestinal parasitism due to co-infection with oxyurids and ascarid nematodes in the captive population of Indian star tortoise maintained at Zoological Garden, Thiruvananthapuram, Kerala, India. The diseased tortoise exhibited clinical signs like diarrhea, anorexia, and weight loss. Direct microscopic examination of pooled fecal sample identified the presence of oxyurid and ascarid ova. Th...
Source: Journal of Parasitic Diseases - March 24, 2020 Category: Parasitology Source Type: research

Climate based malaria forecasting system for Andhra Pradesh, India
AbstractMalaria is a major public health problem in tropical and subtropical countries of the World. During the year 1999, Visakhapatnam district of Andhra Pradesh, India experienced a major epidemic of malaria, and nearly 41,805 cases were reported. Hence, a retrospective malaria surveillance study was conducted from 2001 to 2016 and reported nearly a total of 149,317 malaria cases during the study period. Of which,Plasmodium vivax contributes 32%, andPlasmodium falciparum contributes 68% of the total cases. Malaria cases follow a strong seasonal variation and 70% of cases are reported during the monsoon periods. In the p...
Source: Journal of Parasitic Diseases - March 24, 2020 Category: Parasitology Source Type: research

Seasonal variation of the prevalence of cymothoid isopod Norileca indica (Crustacea, Isopoda), parasitizing on the host fish Rastrelliger kanagurta collected from the Southwest coast of India
AbstractParasitological investigations on the occurrence of isopod parasites in fishes collected from off Cochin coast along Southwest India was carried out for a period of 1  year from January 2018 to December 2018. Altogether 20 species of fishes were analysed from 12 families including Scombridae, Carangidae, Clupeidae, Nemipteridae, Hemiramphidae, Belonidae, Menidae, Priacanthidae, Sphyraenidae, Stromateidae, Coryphaenidae. Infestation ofNorileca indica was noticed only in the host fishRastelliger kanagurta. Totally 619 specimens ofR. kanagurta were examined for the presence of isopod parasite,N. indica. Among tho...
Source: Journal of Parasitic Diseases - March 20, 2020 Category: Parasitology Source Type: research

Modeling an association between malaria cases and climate variables for Keonjhar district of Odisha, India: a Bayesian approach
AbstractMalaria, a vector-borne disease, is a significant public health problem in Keonjhar district of Odisha (the malaria capital of India). Prediction of malaria, in advance, is an urgent need for reporting rolling cases of disease throughout the year. The climate condition do play an essential role in the transmission of malaria. Hence, the current study aims to develop and assess a simple and straightforward statistical model of an association between malaria cases and climate variates. It may help in accurate predictions of malaria cases given future climate conditions. For this purpose, a Bayesian Gaussian time seri...
Source: Journal of Parasitic Diseases - March 19, 2020 Category: Parasitology Source Type: research

A case of Echinococcus granulosus hepatic hydatid cyst together with pyogenic liver abscess in a Nepali patient
AbstractHydatidic disease (echinococcosis) is a significant health problem in Nepal. In humans, mainly involves liver and commonly remains silent without symptoms or causing only mild pressure symptomatology. However, inadequate invasive medical procedures may cause the rupture of the cyst with serious complications, anaphylactic shock and death. Herein, we report the case of a Nepali patient who had been treated of a hepatic abscess with percutaneous drainage and antibiotics in a previous hospitalization. Study by drain fluid, ultrasonography and the magnetic resonance imaging revealed the presence of an intact Hydatidic ...
Source: Journal of Parasitic Diseases - March 18, 2020 Category: Parasitology Source Type: research

Leishmaniasis diagnosis: an update on the use of parasitological, immunological and molecular methods
AbstractDiagnosis of leishmaniasis has always been a major challenge as its clinical features resemble some other commonly occurring diseases such as tuberculosis, typhoid, and malaria. Reliable laboratory methods become important for differential diagnosis. Demonstration of the parasites in stained preparations of bone marrow and splenic aspirates being risky and invasive is still the gold standard for diagnosis. Serological tests utilizing rapid immunochromatographic formats or rK39 in enzyme linked immune sorbent  assay, immunoblotting, direct agglutination test have complications related to high proportions of pos...
Source: Journal of Parasitic Diseases - March 16, 2020 Category: Parasitology Source Type: research

How climate change affects parasites: the case of trematode parasite Clinostomum complanatum and its fish host Trichogaster fasiatus
This study was undertaken to understand the impact of climate change on the ecology of infection ofClinostomum complanatum, a model trematode parasite. We analysed climate change data and data from infected fish over a period of seven years (2007 –2013) from the Aligarh region (India) in this retrospective study. We show that infection of the trematode parasiteClinostomum complanatum (Rudolphi, 1814) in the forage fishTrichogaster facsiatus (Bloch& Schneider, 1801) is dependent on surface air temperature amongst the (ecologically) relevant climate change variables for both the parasite and its host. This study is...
Source: Journal of Parasitic Diseases - March 14, 2020 Category: Parasitology Source Type: research

Haemoparasites in red-legged partridge ( Alectoris rufa ): first record of Haemoproteus sp. in Italy?
AbstractDuring a health survey in the Province of Alessandria (NW Italy) 267 free ranging red-legged partridge (Alectoris rufa; 137 males and 130 females) were captured in 2009 (n  = 101), 2010 (n = 130) and 2011 (n = 36). After biometric data and blood samples collection, animals were released on site. Blood samples were used for the preparation of blood smears. A total of 1.5% of smears presented parasites with a light blue finely granular cytoplasm in red blood cells. The parasites were identified, on the basis of their morphology (no molecular identification was possible), as b...
Source: Journal of Parasitic Diseases - March 13, 2020 Category: Parasitology Source Type: research

Molecular and epidemiological characterization of Giardia Intestinalis assemblages detected in Djelfa, Algeria
In conclusion, this study gave the first molecular data onG. intestinalis isolates in the city of Djelfa. Further expanded studies using more genes and covering other cities in Algeria are mostly needed. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - March 13, 2020 Category: Parasitology Source Type: research

Apicomplexan lineage-specific polytopic membrane proteins in Cryptosporidium parvum
AbstractApicomplexans are a group of parasitic protozoans, includingPlasmodium andCryptosporidium species, which harbor a specialized organelle called an apicoplast. Of the 145-apicomplexan lineage-specific proteins identified inCryptosporidium parvum, 30 are surface proteins. InPlasmodium falciparum, a heteromeric complex of three related apicomplexan lineage-specific membrane proteins containing 6 transmembrane domains (m6t) have been identified. These proteins are Pfm6t α, Pfm6t β, and Pfm6t γ and these proteins are localized on merozoite as an inner membrane complex (Rayavara et al. in Mol Biochem Para...
Source: Journal of Parasitic Diseases - March 13, 2020 Category: Parasitology Source Type: research

Antiplasmodial activity of Cocos nucifera leaves in Plasmodium berghei -infected mice
In this study, we aimed to study the in vivo performance of the decoction ofC. nucifera leaves inP. berghei-infected mice. We analyzed the effectiveness of different routes of administration and the acute toxicity of the extract. Additionally, we determined the suppressive, curative and prophylactic activity of the extract. The results showed that the decoction of leaves ofC. nucifera is most effective when administered intramuscularly to mice in comparison to intraperitoneal, subcutaneous and intragastric methods. We also found that organ signs of acute toxicity appear at 2000  mg/kg/day as evidenced by necropsy exam...
Source: Journal of Parasitic Diseases - March 9, 2020 Category: Parasitology Source Type: research

Gastrointestinal parasites in the opossum Didelphis aurita : Are they a potential threat to human health?
AbstractCurrently, a great proportion of the emerging infectious human diseases are zoonotic, with most of the pathogens originated from wildlife. In this sense, synanthropic animals such as marsupials play important role in the dissemination of pathogens due to their proximity to human dwellings. These hosts are affected by many gastrointestinal parasites, including species with zoonotic potential. The aim of this study was to assess the diversity of gastrointestinal parasites infecting the black-eared opossumD. aurita captured in urban areas of Southeastern, Brazil. In addition, the potential risk for the human populatio...
Source: Journal of Parasitic Diseases - March 2, 2020 Category: Parasitology Source Type: research

The first molecular detection of a Theileria -like species (Apicomplexa: Piroplasmida) in Meriones persicus from western Iran
In this study 18S rRNA target gene of piroplasm parasite was amplified by PCR in aMeriones persicus; so, the Locus 1 and Locus 2 of 18S rRNA were sequenced, successfully. ATheileria-like taxa was suspected in accordance with the BLAST analysis of 18S rRNA L1 and L2 with 96% and 91% sequence homology, respectively. The present study was the first report of aTheileria-like species inM. persicus from Iran. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - February 28, 2020 Category: Parasitology Source Type: research

In vitro and in vivo analysis of the anti-plasmodial activity of ethanol extract of Phyllanthus nivosus W. Bull leaf
AbstractAntimalarial agents are necessary tools in the global malaria eradication agenda and plants used traditionally in the treatment of malaria are indispensable sources of antimalarial compounds. The aim of this study was to evaluate the antiplasmodial potential ofPhyllanthus nivosus leaf. In vitro antiplasmodial assay was conducted usingPlasmodium falciparum infected erythrocytes incubated at 37  °C in modified RPMI 1640 culture media. The inhibitory effect of the ethanol extract on plasmodium lactate dehydrogenase (pLDH) activity was determined as a measure of antiplasmodial activity. In vivo study was done ...
Source: Journal of Parasitic Diseases - February 28, 2020 Category: Parasitology Source Type: research

Prevalence and financial loss estimation of parasitic diseases detected in slaughtered cattle in Kwara State, North-central Nigeria
This study was conducted to determine the prevalence and the economic loss from carcass and viscera condemnation due to parasitic diseases in slaughtered cattle in Kwara State, North-central Nigeria. A 16  year (2003–2018) record of parasitic diseases detected in cattle slaughtered at the major abattoirs in Ilorin city was retrieved from the Kwara State Veterinary department of the Ministry of Agriculture and Natural Resources. The total prevalence as well as the yearly, monthly and seasonal preva lence of each of the documented parasitic diseases was determined. The financial losses associated with each of the ...
Source: Journal of Parasitic Diseases - February 28, 2020 Category: Parasitology Source Type: research

New host and new geographical record of the ciliate parasites of ornamental fish, Botia rostrata from Arunachal Pradesh for the first time in India
AbstractOrnamental fish culture is growing to a prosperly in India. India earned lot amount of money from this business but unfortunately the ornamental fishes are facing lots of problem due to the parasitic infestation. While surveying, some ciliate parasites namely,Trichodina oligocotti (Lom in Arch Protistenkd 112:153 –177, 1970) andTripertiella bulbosa (Davis in US Dept Interior Fish Bull 51:1 –29, 1947) Lom (Acta Parasitol Polon 7:573–590, 1959) have been identified from ornamental fishBotia rostrata from Arunachal Pradesh, India. The species have been identified according to the guidelines of Lom (J...
Source: Journal of Parasitic Diseases - February 28, 2020 Category: Parasitology Source Type: research

Prevalence and economic impact of cystic echinococcosis and liver fluke infections in slaughtered sheep and goat in north-central Iran, 2008 –2018
This study aimed to analyze the 11-year record of offal condemnation due to CE, fascioliasis, dicrocoeliasis infections in sheep and goat and its economic impact at Alborz slaughterhouse, north-central Iran. The prevalence rate was calculated as the infected organs (as nominator) divided by the slaughtered cases (as the denominator) in each year and month. The annual percent changes was used to determine trends of parasitic diseases over time. The relationship between metrological indexes and the prevalence of parasitic diseases was determined by the linear regression model. Statistical analyses were done using STATA softw...
Source: Journal of Parasitic Diseases - February 28, 2020 Category: Parasitology Source Type: research

Parasitological and histopathological diagnosis of a non-native fish ( Oreochromis sp.) with a noticeable presence in a natural Brazilian river environment
AbstractNile tilapia is an exotic species produced in Brazilian aquaculture. The aim of this study was to constitute a pathological diagnosis inOreochromis sp. with a noticeable presence in a river in southern Brazil, and to verify the origin of these fish in this natural habitat, an environment with high sanitary precariousness due to discharges of industrial effluents. A total of 30 fish were collected during three sampling events and their health status was assessed by parasitological fauna survey, parasitological indices and histopathological examination of the gills, liver, spleen and kidney. Five species of monogenea...
Source: Journal of Parasitic Diseases - February 28, 2020 Category: Parasitology Source Type: research

Morphologic and molecular analysis of Dicrocoelium dendriticum species from Iranian native sheep
AbstractDicrocoeliosis is a worldwide parasitic disease of ruminants which affects the liver. In this current study, the phylogenic pattern ofDicrocoelium species in Iranian native sheep from three different geographical regions was investigated by investigating a 520  bp fragment of mitochondirial NAD1 gene. The analysis of the NAD1 oligo nucleotide sequences from 10D. dendriticum (GenBank accession numbers: MG889399 to MG889408) revealed few non-significant differences, suggesting limited application for NAD1 gene as a biomarker for study of genetic variation inDicrocoelium. The morphometrical study also showed a si...
Source: Journal of Parasitic Diseases - February 18, 2020 Category: Parasitology Source Type: research

Investigation of the in vitro cysticidal activity of miltefosine against Acanthamoeba spp.
AbstractThe present study evaluated the in vitro efficacy of miltefosine against cysts ofAcanthamoeba spp. belonging to genotypes T3, T4 and T5. Each genotype was incubated with miltefosine at the concentration of 2.42, 4.84, 9.68, 19.36, 38.72 and 77.44  mM for different periods; 1, 3, 5, 7 d at 37 °C. The viability was assessed by staining with 0.4% trypan blue and culturing on NNA medium at 30 °C for 1 month. The results showed 100% eradication of cyst stage of all concentrations, but exhibited a different degree of activity against differe nt genotypes. The MCC of 38.72 mM could kill ge...
Source: Journal of Parasitic Diseases - February 17, 2020 Category: Parasitology Source Type: research

Additional morphometric and phylogenetic studies on Mothocya melanosticta (Isopoda: Cymothoidae) parasitizing the Red Sea Nemipterus randalli fish in Egypt
AbstractCymothoidae, Leach, 1818 (Crustacea: Isopoda) are ectoparasites of marine, brackish and freshwater fishes that are reported to induce deleterious tissue impacts on the infested fish hosts. The present study aimed for the first-time screening ofMothocya melanosticta collected from the red sea fishNemipterus randalli in Egypt. Surveillance study was conducted for isopod infestation among the Red Sea fishN. randalli revealing a total infestation rate of 40.96% with the species identified asM. melanosticta. The parasite species was isolated from the branchial cavity and gills. Morphometric description was estimated usi...
Source: Journal of Parasitic Diseases - February 12, 2020 Category: Parasitology Source Type: research

Comparison of camel, dog and the laboratory animals ’ sera with the fetal calf serum (FCS) for cultivation of Leishmania major
This study was conducted to compare various laboratory (BALB/c mice, rat, rabbit, hamster and guinea pig) and non-laboratory (dog and camel) animals ’ sera as a substitute for FCS inL. major culture.L. major,MRHO/IR/75/ER strain, was cultivated in RPMI-1640 medium enriched with different percentages of mentioned animal ’s sera. Parasite growth was checked constantly. The rate of growth and survival of parasites were compared with a control medium enriched with FCS. As well, biochemical (albumin, globulin AST, ALT, ALP, Bil, BUN, Crea, Ca, P, Na, K, Fe, TIBC, Mg, zinc, Chol, HDL, LDL, TG, BS, uric acid, LDH, CPK...
Source: Journal of Parasitic Diseases - February 8, 2020 Category: Parasitology Source Type: research

The effect of a Local isolate and Houghton strain of Eimeria tenella on clinical and growth parameters following challenge in chickens vaccinated with IMMUCOX ® and LIVACOX ® vaccines
AbstractCoccidiosis, a parasitic infection caused by one or more of the numerous species ofEimeria is recognised as the disease that has severe economic impact on poultry production. In an experiment to determine the effect of vaccination on the infection with a Local isolate and Houghton strain ofEimeria tenella (E. tenella). Ninety (90) day-old Dominant black cockerel chicks of D109 strain were randomly divided into nine groups (A –I). Two commercially available anti-coccidial vaccines—Livacox® (Biopharm, Czech Republic) and Immucox® (Vetech, Ontario, Canada) were used to immunize the chicks. Immuniza...
Source: Journal of Parasitic Diseases - February 7, 2020 Category: Parasitology Source Type: research

Prevalence of Angiostrongylus cantonensis among different species of snails in the village of Bagong Sikat Mu ñoz, Nueva Ecija, Philippines and its associated risk factors for zoonotic transmission
AbstractSnails are good source of protein and one of the delicacies in majority of the areas in the Philippines. However, they may act as an intermediate host for several parasites such asAngiostrongylus cantonensis. This parasite is commonly found in South East Asia especially in agricultural countries like the Philippines. Hence, the present study aims to determine the prevalence of A. cantonensis in their intermediate host in Village Bagong Sikat, which is situated at the rice granary of the Philippines. A total of 947 snails were randomly collected within 50  m × 50 m transect line through...
Source: Journal of Parasitic Diseases - February 3, 2020 Category: Parasitology Source Type: research

Two new host records of the cymothoid isopods from Rameswaram Island, southeast coast of India
AbstractCymothoid isopods are parasitic crustaceans that are considered as a potential threat to the health of different fish species, which eventually lead to economic loss to the fisherman ’s society. For the first time, cymothoid isopodsNerocila sundaica andNerocila poruvae infested on the two new host fish species viz.,Eleutheronema tetradactylum andSetipinna taty, which were collected from Rameswaram Island, Southeast coast of India. Being an ectoparasite,N. sundaica was found attached on the gill chamber ofE. tetradactylum, whileN. poruvae was attached on the body surface ofS. taty. The standard length 10 &ndas...
Source: Journal of Parasitic Diseases - February 1, 2020 Category: Parasitology Source Type: research

Quantification of Eimeria necatrix , E. acervulina and E. maxima genomes in commercial chicken farms by quantitative real time PCR
AbstractAdvent of quantitative polymerase chain reaction and its variants have enabled identification and quantification of seven knownEimeria species of poultry in biological samples. Attempts were made in the present study to identify and quantify three important pathogenicEimeria species responsible for intestinal coccidiosis in domestic farmed chicken,E. necatrix, E. acervulina andE. maxima in droppings collected from thirty one poultry farms of North Indian states of Haryana, Punjab, Uttar Pradesh and Uttarakhand. The study included broiler, layer and backyard rearing units. Overall occurrence ofE. necatrix,E. maxima ...
Source: Journal of Parasitic Diseases - January 29, 2020 Category: Parasitology Source Type: research

Genotyping the causative agents of cutaneous leishmaniasis in Ahvaz southwest of Iran
AbstractWith sand flies as the main vectors,Leishmania species cause ancient zoonotic diseases called leishmaniasis. Iran is an endemic country regarding cutaneous leishmaniasis. A number of 100 smear slides were collected from cutaneous lesions referred to Ahvaz health centers. The DNA was extracted and ITS1 –PCR using LITSR and L5.8S primer pair was performed to detect the genusLeishmania. Then, enzymatic digestion of PCR products was done byHaeIII (species detection),TaqI (strain detection),DpnI andHpaII (mutation assessment). Furthermore, 50 samples were sent for sequencing. Microscopic examination showed amastig...
Source: Journal of Parasitic Diseases - January 24, 2020 Category: Parasitology Source Type: research

Reliability of heterophyid antigens in heterologous protection against human schistosomiasis
AbstractSchistosomiasis is a neglected tropical parasitic disease which has been controlled by praziquantel for many decades; however, reemergence of praziquantel resistant strains has been a continuous threat. Therefore, the development of reliable antischistosomal vaccine is significantly demanded for optimal control. In the present study, comparison amongSchistosoma haematobium, Schsitosoma mansoni andPygidiopsis genata crude antigens was carried out by Sodium dodecyl sulphate polyacrylamide gel electrophoresis. Hyperimmunization of rabbits with tested parasites ’ crude antigens was done to obtain hyperimmune sera...
Source: Journal of Parasitic Diseases - January 24, 2020 Category: Parasitology Source Type: research

Redescription of Tanqua tiara (von Linstow, 1879) Blanchard, 1904 (Nematoda: Gnathostomatidae) from Varanus flavescens (Hardwicke and Gray, 1827) (Reptilia: Varanidae) from Birbhum district, West Bengal, India
AbstractTanqua tiara (von Linstow, 1879) Blanchard, 1904, recovered from the stomach of water monitor lizard,Varanus flavescens (Hardwicke and Gray, 1827), collected from the Dubrajpur town, Birbhum district of West Bengal, India, is redescribed with light as well as scanning electron microscopy. This species is characterised by presence of two large lateral pseudolabia followed by massive cephalic bulb. The inner surface of each pseudolabium is folded into five rounded tooth-like formations which fit into corresponding folds on adjacent pseudolabium. Spicules are equal and similar. Gubernaculum is absent. Caudal papillae ...
Source: Journal of Parasitic Diseases - January 23, 2020 Category: Parasitology Source Type: research

The first record on Giardia muris from mice in Turkey
AbstractGiardia muris has been reported from both laboratory and wild rodents in worldwide. During routine care, soft consistency was observed in feces of Swiss albino mice. MotileGiardia spp. trophozoites were observed microscopically on all fecal preparations after stained with Lugol ’s iodine solution. The trophozoites were broadly ovoid in a form with a pointed end. They possess two large nuclei and an adhesive disk whose length overlapped one-half of the trophozoite body length. The caudal flagella are unequal. β giardin (bg) gene region of isolates was successfully amplified and sequenced. According to seq...
Source: Journal of Parasitic Diseases - January 21, 2020 Category: Parasitology Source Type: research

New records of hosts for Excorallana longicornis and Nerocila acuminata (Crustacea: Isopoda) in brackish fish from the coast of the State of Amap á (Brazil), with an update on the geographic distribution of Nerocila acuminata
AbstractFish are parasitized by several species of crustaceans, including Cymothoidae and Corallanidae. The aim of this study was to investigate the crustacean parasite fauna inAnableps anableps,Amphiarius rugispinis,Bagre bagre,Cathorops spixii,Cynoscion acoupa,Centropomus undecimalis,Macrodon ancylodon,Mugil curema, Megalops atlanticus,Pseudachenipterus nodosus,Plagioscion squamosissimus,Platystacus cotylephorus,Sciades passany,Sciades herzbergii, andHypostomus ventrimaculata from the coast of the State of Amap á, eastern Amazon. In addition, an update on the geographic distribution ofNerocila acuminata in Brazili...
Source: Journal of Parasitic Diseases - January 13, 2020 Category: Parasitology Source Type: research