Human Toxocariasis in individuals with blood disorders and cancer patients: the first seroepidemiological study in Iran
This study was designed to evaluate the seropositivity of Toxocara IgG in patients with blood disorders and cancer patients in southwest Iran. Moreover, the study of the associated risk factors for this infection. A total of 1122 serum samples, from February 8, 2019 to August 21, 2019, including 600 healthy individuals and 522 individuals with cancer and blood disorders patients were collected. Serum samples were collected for detection of Toxocara IgG by using ELISA (Enzyme-Linked Immunosorbent Assay) kit. Sociodemographic data of all participants were collected and examined to determine their association with the infecti...
Source: Journal of Parasitic Diseases - January 16, 2021 Category: Parasitology Source Type: research

Diet, parasites, and other pathogens of Sunda leopard cats ( Prionailurus javanensis Desmarest 1816) in Aborlan, Palawan Island, Philippines
This study is the first investigation of parasites and other pathogens present in Sunda leopard cats (Prionailurus javanensis) in Aborlan, Palawan, Philippines. With the nature of wild carnivore sampling, four (4) wild Sunda leopard cats were captured in Aborlan, Palawan, Philippines for a period of nine (9) months. Of these, three (3) were considered for blood and fecal examination due to the poor condition of one animal. Rapid diagnostic kits were used to detect the presence of selected pathogens in blood samples while fecal samples were examined for parasite fauna and diet contents. Nine (9) parasite species were identi...
Source: Journal of Parasitic Diseases - January 8, 2021 Category: Parasitology Source Type: research

Paragonimus westermani infection of freshwater crab Sundathelphusa philippina and melaniid snails in Cadacan River in Irosin, Sorsogon, Philippines
This study aimed to investigate the infection status and risk factors ofP. westermani in freshwater crabs and melaniid snails collected in an endemic village along Cadacan River in Irosin, Sorsogon, where human cases of paragonimiasis were previously reported. A total of 246 freshwater crabs (118 females, 128 males) were dissected, and the gills, muscles, gonads, and viscera were examined for the presence of metacercariae; of which, 41.87% were found infected. The metacercariae were recovered from the gills (100%) and muscle tissues (7.3%) of infected crabs. Male crabs were more likely to be infected (49.22%) than female c...
Source: Journal of Parasitic Diseases - January 8, 2021 Category: Parasitology Source Type: research

Diagnosing the drug resistance signature in Plasmodium falciparum : a review from contemporary methods to novel approaches
AbstractThe genome sequence project of the human malaria parasitePlasmodium falciparum reveal variations in the parasite DNA sequence. Most of these variations are single nucleotide polymorphism (SNP). A high frequency of single nucleotide polymorphism (SNP) in the  Plasmodium falciparum population is usually a benchmark for anti-malarial resistance which allows parasites to be elusive to the chemotherapeutic agents, vaccine and vector control strategies, resulting in the leading cause of morbidity and mortality globally. The high density of drug resistance signature markers such aspfcrt,pfmdr1, pfdhps, pfdhfr,pfkelch...
Source: Journal of Parasitic Diseases - January 7, 2021 Category: Parasitology Source Type: research

Assessment of chitosan nanoparticles in improving the efficacy of nitazoxanide on cryptosporidiosis in immunosuppressed and immunocompetent murine models
AbstractCryptosporidiosis is one of the major causes of diarrhea in immunocompetent and immunocompromised patients. It is self-limited in immunocompetent individuals. However, in the immunocompromised it can cause life-threatening diarrhea and results in chronic malabsorption of fluids, vitamins and electrolytes resulting in wasting. Our study is concerned with assessing and comparing the efficacy of nitazoxanide (NTZ) alone and NTZ loaded chitosan nanoparticles (NTZ loaded CS NPs) in the treatment of experimental cryptosporidiosis using parasitological and histopathological parameters. One hundred mice were divided into 5...
Source: Journal of Parasitic Diseases - January 6, 2021 Category: Parasitology Source Type: research

Prevalence and severity of gastro-intestinal parasites in buffalo calves at Sylhet division of Bangladesh
AbstractGastrointestinal (GI) parasites are one of the most widely recognized health problems of buffalo calves in Bangladesh. The study was undertaken to investigate the prevalence of GI parasitic infestation in buffalo calves at Sylhet division of Bangladesh. Fecal samples of 200 buffalo calves under 1  year of age (76 males and 124 females) were collected from Sylhet, Maulvibazar, Habiganj, and Sunamganj districts, and from each of the four regions, 50 samples were collected. Samples were examined through simple flotation, sedimentation, and McMaster techniques. The overall prevalence of GI para sites in Sylhet div...
Source: Journal of Parasitic Diseases - January 6, 2021 Category: Parasitology Source Type: research

Lamb ’s Eimeria infections raised in a steppic region and their impacts on clinical indicators (FAMACHA © and Disco)
The objective was to identify by the microscopic morphology of oocysts theEimeria species that infect lambs in a steppe region of eastern Algeria, as well as to evaluate on these parasites two clinical indicators of diarrhea (Disco) and anemia (FAMACHA ©) used in targeted selective treatments against gastrointestinal nematodes (GIN). This present work completes the previous evaluations carried out in steppe conditions in our laboratory on GIN and cestodes. All these enteric parasites can influence these indicators. They constitute by their morbid ity and their presence, alone or concomitantly, a factor determining or ...
Source: Journal of Parasitic Diseases - January 6, 2021 Category: Parasitology Source Type: research

Effect of polyphenols extracted from Punica granatum and Acacia saligna plants on glutathione S-transferase of the cattle tick Rhipicephalus ( Boophilus ) annulatus (Acari: Ixodidae)
The objective of this study was to evaluate the effects ofPunica granatum red peel andAcacia saligna leaf extracts onRhipicephalus(Boophilus) annulatus GST activity in order to reduce the resistance of cattle to acaricide. The results showed thatP. granatum ethanol extract (70%) contained the highest total phenol content (350  ± 1.2 μM GAE g−1), the highest condensed tannin content (270  ± 1.3 μM CE g−1) and the highest hydrolysable tannin content (70  ± 5.0 μM TAE g−1). Adult immersion test with a dosage of 100  m...
Source: Journal of Parasitic Diseases - January 5, 2021 Category: Parasitology Source Type: research

Comprehensive analysis of oral administration of Vitamin E on the early stage of Trypanosoma brucei brucei infection
In conclusion, the treatment was able to ameliorate the anaemia and organ damage caused byTrypanosoma brucei brucei, extend the life span of the treated rats and greatly delay the time taken to get to the second stage of the infection. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - January 3, 2021 Category: Parasitology Source Type: research

Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array
The objective of this study was to evaluate the applicability of DBS with a commercial multiplex malaria assay, developed to concurrently measurePlasmodium antigens, histidine-rich protein 2 (HRP2),Plasmodium lactate dehydrogenase (pLDH), and a host inflammatory biomarker, C-reactive protein (CRP), in whole blood. The assay conditions were optimized for DBS, and thermal stability for measurement ofPlasmodium antigens and CRP in dried blood were determined. Performance of the multiplex assay on matched DBS and whole blood pellet samples was also evaluated using the clinical samples. The results indicate the acceptable perfo...
Source: Journal of Parasitic Diseases - January 3, 2021 Category: Parasitology Source Type: research

Comparative pathology of mice infected with high and low virulence of Indonesian Trypanosoma evansi isolates
AbstractMice infected withT. evansi cause various clinical manifestations and histopathological changes. The aim of this study was to compare the histopathological lesions of mice infected withT. evansi Bang 87 isolates (high virulence) and Pml 287 isolates (low virulence). A total of 15 susceptible mice (DDY) were divided into three groups (five mice/group): Groups I and II each were infected with 104T. evansi of high virulence (Bang87) and low virulence (Pml 287), respectively, whereas group III served as a control group. A total of three mice from group I, and one mouse from each group II and III were killed at 4 dpi. A...
Source: Journal of Parasitic Diseases - January 3, 2021 Category: Parasitology Source Type: research

Accumulation of nine heavy metals in water and gills, intestine and digenean parasites of the silver catfish, Bagrus bajad Forsk ål, 1775
AbstractBioindicator value of digenean parasites (Acanthostomum absconditum,Acanthostomum spiniceps andHaplorchoides cahirinus) of silver catfish,Bagrus bajad was assessed by analyzing the concentrations of nine heavy metals (Fe, Zn, Cu, Ni, Cd, Cr, Mn, Co, and Pb) using atomic absorption spectrometry. Additionally, the study compared heavy metals concentrations in four sinks (water, gills, intestine and digenean worms) from two aquatic habitats (the upstream and downstream) of the Damietta Branch of the River Nile from January to December, 2018. The results revealed that iron and manganese were the most abundant metals in...
Source: Journal of Parasitic Diseases - January 3, 2021 Category: Parasitology Source Type: research

Occurrence of ectoparasitic isopod Norileca indica (H. Milne Edwards, 1840) on bigeye scad Selar crumenophthalmus (Bloch, 1793) from Great Nicobar Island, India
AbstractPresent study records the occurrence and range extension of an ectoparasitic isopod,Norileca indica (H. Milne Edwards, 1840) from the branchial cavity of bigeye scadSelar crumenophthalmus (Bloch, 1793) collected from Pilobah, Great Nicobar Islands. Detailed morphological characteristic of the parasite were presented along with prevalence, mean intensity and abundance. Out of the 130 specimens ofS. crumenophthalmus examined, 32 were found to be infested withN. indica. The sizes of the female specimens were in the ranges 14.0 to 20.1  mm and the male specimens were 13–14 mm. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - January 3, 2021 Category: Parasitology Source Type: research

Improvement of a PCR-based method for the detection of Opisthorchis viverrini eggs in human stool samples by targeting internal transcribed spacer-2 (ITS-2), cytochrome oxidase subunit 1 ( cox1 ), and cytochrome b ( cyb )
This study aims to improve the PCR-based method for detection ofO. viverrini eggs in stool samples by targeting the genes ITS-2,cox1, andcyb. The results reveal higher sensitivity than conventional concentration techniques, with all newly designed primers. ITS-2 has an overall sensitivity of 76.9% with 66.7% in the samples with
Source: Journal of Parasitic Diseases - January 2, 2021 Category: Parasitology Source Type: research

Aspergillus niger trehalase enzyme induced morphological and protein alterations on Acanthamoeba cyst and molecular docking studies
In conclusion, this enzymatic approach could be developed into selective and effective mechanism to controlAcanthamoeba without affecting the host especially mammals due to the absence of trehalose elements in the tissues of mammals. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - January 2, 2021 Category: Parasitology Source Type: research

Extremely low prevalence in soil-transmitted helminth infections among a multi-ethnic community in Segamat, Malaysia
AbstractSoil-transmitted helminth infections (STHs) are recognized as a major health issue among socio-economically deprived communities. However, information is still lacking regarding the prevalence rates of STHs in the broader community across different countries in the tropics. This community study aimed to determine the prevalence and risk factors for STHs in semi-rural communities in Segamat of Johor, Malaysia. A cross-sectional study was conducted with information collected from the study population through questionnaire. A total of 224 stool samples were examined for intestinal parasites through formalin-ether conc...
Source: Journal of Parasitic Diseases - January 1, 2021 Category: Parasitology Source Type: research

Taenia hydatigena in goat and sheep in Ghana: a cross-sectional abattoir survey in Northern and Upper West Regions
AbstractParasitic infections are a major setback to livestock production in Ghana but the disease situation in many localities is poorly understood. A study to determine the prevalence and distribution ofTaenia hydatigena in goats and sheep was carried out in two major municipal abattoirs in Tamale and Wa. A total of 538 goats and 256 sheep were screened for cysts during a cross-sectional survey which revealed a prevalence of 22.34% and 33.96% in goats from Tamale and Wa, respectively, and 22.66% in sheep from Tamale. Infection was common in male and female, young and old animals alike. Animals considered in the study orig...
Source: Journal of Parasitic Diseases - January 1, 2021 Category: Parasitology Source Type: research

Morphotaxonomical description of six new species of acephaline gregarines infecting oligochaete host
AbstractThis communication deals with the morphology, taxonomy and life history of six new acephaline gregarines (Apicomplexa: Gregarinomorphea) of the genusMonocystis Stein (1848),Nematocystis Hesse (1909),Aikinetocystis Gates (1926) andDirhynchocystis Cognetti de Martiis (1921) from oligochaete hosts. These areMonocystis molnari sp. nov. fromMetaphire anomala (Clitellata: Opisthopora: Megascolecidae);Monocystis paneki sp.  nov. fromLampito mauritii (Clitellata: Opisthopora: Megascolecidae);Monocystis bulbae sp.  nov fromMetaphire posthuma (Clitellata: Opisthopora: Megascolecidae);Nematocystis cylindrum sp. &nbs...
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Histopathological and biochemical studies on immunocompetent and immunocompromised Hymenolepis nana infected mice treated with Commiphora molmol (Mirazid)
AbstractHymenolepiasis is a common parasitic infection of children and considered an opportunistic disease with a life-threatening effect among immunocompromised patients through cysticercoid dissemination. Mirazid (Commiphora molmol) is new phytotherapy for hymenolepiasis. So, the present study aimed to evaluate the effects of Mirazid treatment comparing to Praziquantel (PZQ) (standard treatment) among immunocompetent and immunocompromised infected albino mice through biochemical and histopathological parameters. A total of 60 clean male albino mice (immunocompetent group) were divided into equal five groups which were in...
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Advanced immunological studies on Cephalopina titillator with special references to the epidemiological uses of Dot-ELISA in camel sera
This study aimed to evaluate two new immunologic diagnostic techniques; indirect-ELISA and Dot-ELISA, for the screening ofC. titillator infestation in camels. Thirty slaughtered camel heads were examined carefully for the presence ofC. titillator larvae. One hundred, third-stage larvae (L3), were dissected for the collection of their salivary glands, for the preparation of the salivary gland antigen. Blood samples were obtained for hematological and serological examinations. Results revealed a true prevalence ofC. titillator in the sampled camels being 80% (24/30). Infested camels showed a significant reduction in leukocytes (P 
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Ascariasis, Amebiasis and Giardiasis in Mexican children: distribution and geographical, environmental and socioeconomic risk factors
AbstractThe aim of this study is to provide an overview of the geographical distribution of Ascariasis, Amebiasis and Giardiasis, and to identify specific geographical, socioeconomic and environmental factors that are associated with the incidence of these infections in Mexican children. We made use of publicly available data that was reported by federal organizations in Mexico for the year 2010. The contribution of geographical, socioeconomic and environmental factors to the incidence of infections was assessed by a multivariable regression model using a backwards selection procedure.A. lumbricoides incidence was associat...
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Typical features of cutaneous leishmaniasis in the Ilam province, Iran
AbstractSince Cutaneous leishmaniasis is a problematic parasitic infection in the west of Iran, this study was carried out to clarify the epidemiological aspects of Cl. Our database includes all Cl cases with detailed information during 2014 –2018 in Ilam province, west of Iran. Patients referred to health and clinical centers in our study areas then health providers and laboratory technicians took smears to detect leishmania parasites under light microscope. SPSS24 software used for analyzing data by the Chi square test. Mehran and D ehloran districts were alone accounted for 50% of all reported cases. ≥&t...
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis
This study aimed to verify the diagnostic significance of hematological parameters and C-reactive protein (CRP) for toxoplasmosis acute detection. A case-control study was carried out between December 2017 and May 2018, in samples of convenience independent of age and sex. The case group was formed by 25 patients with positive anti-Toxoplasma gondii IgG/IgM antibody and the control group was formed by 21 patients with non-positive anti-Toxoplasma gondii IgG/IgM antibody. The results of the hematological parameters and CRP were analyzed in these patients. The patients withToxoplasma gondii IgM antibody reagent showed higher...
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Molecular characterization of 5S ribosomal spacer sequences of Dirofilaria repens microfilariae in dogs in Kerala, India
AbstractThe present study reports the characteristics of the 5S rRNA spacer sequences ofDirofilaria repens microfilariae isolated from dogs. The 22 nucleotide long spliced leader 1 sequences located in the 5S rRNA spacer region are completely conserved in all nematodes. There is variation in the spliced leader 1 sequences and associated sites in the 5S rRNA spacer region ofD. repens. Absence of canonical SL 1 sequences distinguishesD. repens from other filarial species. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - October 30, 2020 Category: Parasitology Source Type: research

Identification and incidence of hard tick species during summer season 2019 in Jijel Province (northeastern Algeria)
AbstractTicks are haematophagous arthropodsand tick-borne diseases causes billions of dollars in losses in the cattle industry because of the ability of ticks to be vector for many pathogens. This paper identifies and compares the infestation rate (prevalence and intensity) of females of hard tick species compared to males with respect to sex, age and breed of cattle in the region of Jijel, northeastern Algeria. A total of infested 53 cattle were screened and 1214 ticks were collected manually during summer 2019 (June, July and August) and identified based on their morphology. Seven tick species were identified as infestin...
Source: Journal of Parasitic Diseases - October 28, 2020 Category: Parasitology Source Type: research

Antagonistic effects of native strains of the soil fungus Paecilomyces against gastrointestinal nematode and protozoan parasites of pigs in Panama
The objectives of this exploratory and observational study were: (1) to isolate and cultivate natives species ofPaecilomyces from natural soils in Panama, and (2) to evaluate isolated strains for their capacity to parasitize endemic gastrointestinal nematode and protozoan parasites recovered from naturally infected domestic pigs by observing cultures for spore adhesion and hyphae penetration phases. Using microcultivation and inoculation techniques, four strains ofPaecilomyces were isolated from three locations in Panama, out of which three successfully adhered to and penetrated free-living stages (eggs, cysts and oocysts)...
Source: Journal of Parasitic Diseases - October 27, 2020 Category: Parasitology Source Type: research

Genotyping and phylogenetic analysis of hydatid cysts isolated from livestock in Bushehr province, Iran
AbstractHydatid cyst is one of the parasitic zoonoses caused by infection with the larval stage ofEchinococcus granulosus tapeworm. The spread of this parasite is global and is of great importance in terms of public health. To date, ten different species of this parasite have been identified that differ in characteristics such as life cycle, epidemiology and pathogenesis. The purpose of this study was to determine the genotype and phylogenetic relationship of hydatid cysts isolated from livestock of Bushehr province, Iran. About 62 samples of hepatic and pulmonary hydatid cysts were collected from slaughtered animals. DNA ...
Source: Journal of Parasitic Diseases - October 26, 2020 Category: Parasitology Source Type: research

Identification of Leishmania species by high-resolution melting analysis in newly emerged foci in Sabzevar, northeast of Iran
AbstractCutaneous leishmaniasis (CL) is a zoonotic disease with 1 to 1.5 million annual incidences. Microscopic examination of the Giemsa stained slides is the most common diagnostic method for CL. However, this method cannot distinguish leishmania species. Hence the present study was conducted to identify leishmania species by high-resolution melting (HRM) analysis in the newly emerged foci of CL in Sabzevar, northeast of Iran. In this cross-sectional study, fifty patients with suspicious cutaneous lesions referring to the designated health center for diagnosis and treatment of CL in Sabzevar during 2017 –2018, were...
Source: Journal of Parasitic Diseases - October 24, 2020 Category: Parasitology Source Type: research

Acquired antibody responses against merozoite surface protein-1 19 antigen during Plasmodium falciparum and P.vivax infections in South Indian city of Mangaluru
AbstractMerozoite surface protein-1 (MSP-1) of malaria parasites has been extensively studied as a malaria vaccine candidate and the antibody response to this protein is an important indicator of protective immunity to malaria. Mangaluru city and its surrounding areas in southwestern India are endemic to malaria withPlasmodium vivax being the most widespread and prevalent species althoughP. falciparum also frequently infects. However, no information is available on the level of protective immunity in this population. In this regard, a prospective hospital-based study was performed in malarial patients to assess antibody re...
Source: Journal of Parasitic Diseases - October 21, 2020 Category: Parasitology Source Type: research

Preliminary detection of mites and coccidia with their zoonotic potential in meat-farmed rabbits in three districts in Selangor, Malaysia
In conclusion, mites and coccidia are commonly present in the com mercial rabbit farms, thus control and preventive measures should be executed to reduce the incidence of parasites. The zoonotic mitesSarcoptes scabiei andCheyletiella parasitovorax detected in this study could be regarded as a public health concern especially when handling the rabbit. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - October 19, 2020 Category: Parasitology Source Type: research

In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp . cysts
This study evaluated in vitro effect of different concentrations ofAloe vera (A. vera) ethanol extract and honey againstAcanthamoeba spp. cysts in comparison with chlorhexidine (the drug of choice for treatment ofAcanthamoeba infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600  μg/ml forA. vera ethanol extract and 25, 50, 100, and 200  μg/ml for honey. Isolated Acanthamoeba spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different...
Source: Journal of Parasitic Diseases - October 15, 2020 Category: Parasitology Source Type: research

Serum levels of CC chemokine ligands in cutaneous leishmaniasis patients
This study aimed to assess the serum levels of CC ligand (CCL) 2, CCL5, and CCL11 in cutaneous leishmaniasis (CL) patients. 64 patients, suffering from CL and 100 healthy people were selected, and their blood serum concentrations of CCL2, CCL5, and CCL11 were measured using enzyme-linked immunosorbent assay. The results demonstrated that while the mean serum levels of CCL5 and CCL11 increased significantly in CL patients, the mean serum levels of CCL2 decreased, compared to the control group. Despite the sufficient production of CCL5 and CCL11 in CL patients, they suffered from CCL2 deficiency, as the defense mechanism aga...
Source: Journal of Parasitic Diseases - October 14, 2020 Category: Parasitology Source Type: research

Prevalence of ticks (Acari: Ixodidae) and Theileria lestoquardi in sheep at El Huda and El Nuhud animals production research stations, Sudan
This study revealed that 13% of sheep were suffering fromTheileria lestoquardi which is a considerable number at the stations. Therefore, further epidemiological investigations on disease throughout the year are required in order to set a well-coordinated control program. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - October 13, 2020 Category: Parasitology Source Type: research

Efficacy of diminazene diaceturate and isometamidium chloride hydrochloride for the treatment of Trypanosoma evansi in mice model
AbstractDiminazene diaceturate (DIM) and isometamidium chloride hydrochloride (ISMM) have been widely used for the treatment of animal trypanosomosis. We evaluated the efficacy of standard doses of DIM and ISMM followed by their double doses for the treatment ofTrypanosoma evansi in experimentally infected mice. AT. evansi strain obtained from a naturally infected camel in Afar was used. 25 swiss white mice randomly divided in to five groups were inoculated with 0.2  mL of blood containing 103 trypanosomes. At the peak of parasitemia ( ≈ 2 weeks post infection), groups A and B were treated with the standard...
Source: Journal of Parasitic Diseases - October 12, 2020 Category: Parasitology Source Type: research

Varying levels of protection against Plasmodium falciparium infection were conferred on non-users of long lasting insecticidal nets (LLINS) sleeping in rooms where different number of LLINS were hung in hyper endemic state of West Africa
This study evaluated the residual effect of LLINs on malaria parasite infections among non-LLIN users sleeping in rooms where nets were hung. This study was carried out at Federal University of Agriculture Abeokuta, Ogun State, Nigeria. A total of 259 students were systematically recruited into this study and were classified into two groups of LLINs users and non-users. The LLIN users were further classified according to the proportion of net usage and this includes;    50% respectively. Finger prick blood samples were collected from the participants and microscopically analysed for the presence of parasites....
Source: Journal of Parasitic Diseases - October 12, 2020 Category: Parasitology Source Type: research

Morphological characterization of Moniliformis moniliformis isolated from an Iraqi patient
AbstractInfection withMoniliformis moniliformis is rare in Iraq since it has been recorded only twice by Ministry of Health. In the current study, the morphology of the parasite is evaluated to explain the basic structure of the parasite parasitizing a human body in Iraq, including the adult worm and the egg stage which is considered the diagnostic stage for the detection of the intestinal parasite in the stool sample. The assessment of the adult worm showed that it was white in color and had a pseudo-segmented shape, lacked the digestive system or alimentary canal, and was 133  mm in length. The anterior end bore the...
Source: Journal of Parasitic Diseases - October 12, 2020 Category: Parasitology Source Type: research

Effect of sodium acetate on serum activity of glucose-6-phosphate dehydrogenase in Plasmodium berghei -infected mice
This study, therefore, investigated the effect of sodium acetate on glucose-6-phosphate dehydrogenase inPlasmodium berghei-infected mice. Thirty male Albino mice were randomly distributed into 6 groups, A –F. Animals in Groups B–F were inoculated withP. berghei, intraperitoneally. Subsequently, Group C mice  were treated with 20 mg/kg chloroquine, while groups D, E and F received 25, 50 and 100 mg/kg sodium acetate, respectively. All treatments were administered orally for 4 days. At the end of the experiment, animals were sacrificed by cervical dislocation and blood was collected via&n...
Source: Journal of Parasitic Diseases - October 11, 2020 Category: Parasitology Source Type: research

A critical review of the applicability of serological screening for Leishmaniasis in blood banks in Brazil
This study is a bibliographical review, aiming to understand the sensitivity and specificity parameters of the tests used to detect Leishmaniasis, as well as to understand if there is any relevance in proposing a serological screening for Leishmaniasis in blood banks. In general, we observed that there are currently several types of tests for detecting Leishmaniasis: parasitological, serological and molecular. In such tests, many serological methods and kits are available for the detection of asymptomatic visceral leishmaniasis, but there is variability in sensitivity and specificity among the methods. The gold standard fo...
Source: Journal of Parasitic Diseases - October 8, 2020 Category: Parasitology Source Type: research

Tick infestation on the lower eyelid in an elderly female: a case report
This article presents a case of tick infestation of the lower eyelid. A 58-year-old female presented to Ophthalmology OPD at Nepalgunj medical college with a small whitish lesion on medial aspect of the left lower eyelid since 7  days. On a detailed ocular examination, a tick was found to be masquerading as a pustule. This case report emphasizes the relation of tropical diseases in ophthalmology and the subsequent need of understanding the pathophysiological aspect of tropical diseases to avoid further systemic complicatio ns. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - October 8, 2020 Category: Parasitology Source Type: research

Solid-lipid nanoparticles (SLN)s containing Zataria multiflora essential oil with no-cytotoxicity and potent repellent activity against Anopheles stephensi
In this study, ingredients ofZataria multiflora essential oil (ZMEO) were identified using GC –MS analysis. Solid-lipid nanoparticles containing ZMEO (1%) were prepared (SLN-ZMEO) using the high-pressure homogenizer method. The repellent activity of ZMEO and SLN-ZMEO was investigated using Klun and Debboun method and compared together. Besides, their cytotoxicity on a human skin normal cel l line (HFFF2) was evaluated. Five major components of ZMEO were carvacrol (27.05%), thymol (26.452%), γ-terpinene (15.144%), o-cymene (13.584%), and α-pinene (9.483%). The SLN-ZMEO showed a spherical shape with a parti...
Source: Journal of Parasitic Diseases - October 6, 2020 Category: Parasitology Source Type: research

Intestinal parasites among humans and their livestock animals in a rural community in Gharbia governorate, Egypt
AbstractOur objective was to study the prevalence of intestinal parasitic infections among humans and their contact livestock animals in a rural community in Gharbia governorate, Egypt. The collected human and livestock animal fecal samples were examined by formol ether sedimentation technique and modified Ziehl –Neelsen stain. Human samples were further examined by the Kato–Katz technique. The total prevalence of intestinal parasites in humans was 52.3%; 7.3% helminths and 45% protozoa. Regarding animals, 29% of livestock animals had protozoan parasites and no helminths were detected. Results revealed that ris...
Source: Journal of Parasitic Diseases - October 5, 2020 Category: Parasitology Source Type: research

Trypanosoma cruzi seroprevalence and associated factors in women in Casanare –Colombia
AbstractChagas disease is caused by the parasiteTrypanosoma cruzi and transmitted mainly by triatomines and from mothers to children. In Colombia, this disease is a public health problem and due to its high endemicity and vertical transmission, women are susceptible populations that must be evaluated. Our objective was to determinate theTrypanosoma cruzi seroprevalence and factors associated with women in Pore (Municipality), Casanare, Colombia. Cross-sectional study. A sample of 230 healthy volunteer women, 15  years or older, without previous diagnosis of Chagas disease was taken; the serological analysis was done u...
Source: Journal of Parasitic Diseases - September 30, 2020 Category: Parasitology Source Type: research

The occurrence of parasitic copepods and isopods infesting the marine teleost fishes of Kerala coast, India
AbstractThe present study reports the occurrence of parasitic copepods and isopods infecting marine teleost fishes from the Kerala coast, India. A total of 1795 fishes belonging to 38 species were collected from the fish landing centres across the Kerala Coast for 5  months. The isopod& copepod infection status of these fishes were assessed. The incidents of site-specific infection were documented for all parasites and a fecundity analysis was conducted in randomly selected species. The single or multiple parasitic crustacean infestation and host preference (single or multiple hosts) were also documented. A total ...
Source: Journal of Parasitic Diseases - September 29, 2020 Category: Parasitology Source Type: research

Evaluation of the effect of Peganum harmala extracts on the in vitro viability of Leishmania tropica promastigotes in comparison to Glucantime
This study was conducted to investigate the antileishmanial effect of different extracts ofPeganum harmala grown in Syria, particularly the methanolic extract of seeds and roots as well as its alkaloid fractions onLeishmania tropica promastigotes growth in vitro. After culturing promastigotes and incubating with extracts for 72  h, the surviving promastigotes were counted. The half maximal (50%) inhibitory concentration (IC50) was determined. The experiments were repeated at least three times. The in vitro experiment has demonstrated a concentration-dependent decrease of parasites number caused by the extracts with an...
Source: Journal of Parasitic Diseases - September 26, 2020 Category: Parasitology Source Type: research

Molecular detection of Theileria annulata infection in cattle by conventional PCR and quantitative real time PCR in India
The objective of this study is to identifyT. annulata infection in the peripheral blood of cattle as a biological sample by conventional PCR (cPCR) and quantitative PCR (qPCR). The phylogenetic analysis was done using theT. annulata merozoite surface antigen (Tams 1) gene. Out of 552 samples of examined blood smears by microscopy, 454 (82.24%) animals were positive forTheileria species. Out of 454 samples, 96 samples were further examined by both cPCR and qPCR, 52 samples (54.16%) were found positive forT. annulata in both PCR methodologies. Phylogenetic analysis revealed thatT. annulata Odisha isolate was closely relate...
Source: Journal of Parasitic Diseases - September 24, 2020 Category: Parasitology Source Type: research

Arginase/nitric oxide modifications using live non-pathogenic Leishmania tarentolae as an effective delivery system inside the mammalian macrophages
This study was performed to investigate the effects of IP-10 on the infected human macrophages byL. tarentolae expressing IP-10. We also quantified the arginase activity and NO production in the co-cultured human macrophages withL. tarentolae expressing IP-10 as compared with wildL. tarentolae. The results elucidate that in the infected cells withL. tarentolae expression of IP-10 the arginase activity decreased, and inversely, NO production intensely increased. Altogether,L. tarentolae expressing IP-10 shows a favorable therapeutic tool to improve the treatment ofLeishmania infection. This work suggests thatL. tarentolae e...
Source: Journal of Parasitic Diseases - September 24, 2020 Category: Parasitology Source Type: research

Scolicidal effects of Cassia fistula and Urtica dioica extracts on protoscoleces of hydatid cysts
This study evaluated the therapeutic effects ofUrtica dioica andCassia fistula extracts as a scolicidal herbal drug in vitro. Suspension of protoscoleces was obtained from the infected livers of sheep in Khorramabad, Iran. Hydro-alcoholic solution was extracted from the leaves and stems ofUrtica dioica and the fruit ofCassia fistula.Echinococcus granulosus protoscoleces were treated with the essential oils at concentrations of 10, 25, 50, and 100  mg/mL for 10, 20, 30, and 60 min and their viability was evaluated by the eosin staining test. The extract ofUrtica dioica at a concentration of 100  mg/mL killed ...
Source: Journal of Parasitic Diseases - September 23, 2020 Category: Parasitology Source Type: research

Clinical manifestation and epidemiological findings of human linguatula serrate infection in Iran: systematic review
In this study case reports of individuals in Iran those infected with theLinguatula serrata were taken from the international databases (e.g., Medline (PubMed), Scopus, Iranmedex, Scientific Information Database, and Irandoc) and reviewed. The review covers the case reports of human tongue worm infection published either in Persian or in English between the years 1980 –2018. An individual researcher undertook the bibliographic search and evaluation of articles based on inclusion/exclusion of specific criteria. Cases of infection have been reported in eight provinces of Iran. Dietary habits play the important roles in...
Source: Journal of Parasitic Diseases - September 22, 2020 Category: Parasitology Source Type: research

Prevalence and spatial distribution of the ectoparasites on the gills of Mystus vittatus from River Penna flowing through YSR District, Andhra Pradesh, India
AbstractA parasitic survey was performed during July 2017 to February 2018 to emphasize the prevalence and spatial distribution of ectoparasites inMystus vittatus from three sampling sites of River Penna flowing through YSR District, Andhra Pradesh  by routine parasitological procedures. A total of 238 ectoparasites were obtained from the gills of 70 examined fishes with a prevalence of 77.1%, mean intensity of 4.4 and mean abundance of 3.4 respectively. Three species of ectoparasites i.e., two monogeneans—Cornudiscoides vittati (n  = 119);Bifurcohaptor indicus (n  = 90) and one ...
Source: Journal of Parasitic Diseases - September 20, 2020 Category: Parasitology Source Type: research

Prevalence of heterophyid infection in tilapia fish “ Orechromas niloticus ” with emphasize of cats role as neglected reservoir for zoonotic Heterophyes heterophyes in Egypt
In conclusion, the current work clarifies and highlights a high infection rate of EMC in edible tilapia fish in the study area, also the possible role of cats as a reservoir of the digenetic trematodes particularly the zoonoticH. heterophyes, that necessities planning and application of suitable control measures; and continuous monitoring of fish zoonotic parasites as well as cats and its parasites. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - September 20, 2020 Category: Parasitology Source Type: research