Correction to: Obituary
(Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - May 30, 2022 Category: Parasitology Source Type: research
Infection with Trypanosoma spp. in Platydoras armatulus (Siluriformes, Doradidae), in southwestern Amazon, Brazil
AbstractTrypanosoma is a hemoflagellate capable of infecting a wide variety of invertebrates and vertebrates, such as Neotropical freshwater fish. The present study described and morphologically comparedTrypanosoma spp., found inPlatydoras armatulus, Valenciennes, 1840, in southwestern Amazon. Fish specimens were sampled in Ipixuna and Juru á rivers located in the states of Amazonas and Acre, Brazil. Fish blood samples were taken by cardiac puncture, and smears were prepared for quantification, morphometric measurements, and morphotyping (characterization of the trypanosomes according to their morphological variations) of...
Source: Journal of Parasitic Diseases - May 26, 2022 Category: Parasitology Source Type: research
Prevalence and associated risk factors of Entamoeba histolytica infection among school children from three primary schools in Arsi Town, West Zone, Ethiopia
AbstractEntamoeba histolytica is one of the major pathogenic intestinal parasites and is amongst the leading causes of diarrheal illness in children. Around 500 million people are infected worldwide, while 75,000 die of the disease annually.E. histolytica is associated with moderate to severe diarrhoea and increased mortality among children in African countries and negatively affects child growth and development. Malnutrition is also an important contributor to the prevalence ofE. histolytica in Ethiopia. It is mostly associated with poor hygiene, poverty, illiteracy, lack of access to potable water, and a hot, humid tropi...
Source: Journal of Parasitic Diseases - May 20, 2022 Category: Parasitology Source Type: research
Obituary
(Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - May 20, 2022 Category: Parasitology Source Type: research
Extraction of the DNA of Giardia lamblia isolated from vegetables and fruits in a simplified way and its diagnosis using Nested-PCR
In this study, this parasite was diagnosed based on microscopic diagnosis of isolated samples of vegetables and fruits, as well as molecular diagnosis. The possibility of isolating parasites from vegetables and fruits and extract DNA to be used in most laboratories were verified in this study .The prevalence ofGiardia lamblia were estimated on vegetables and fruits without the need to purchase a special extraction kit. .The prevalence of Giardia on fresh vegetables was estimated by using wet direct swab with Lugol ’s iodine dye in microscopic examination, at a rate of 4 positive samples out of 230 of all studied species,...
Source: Journal of Parasitic Diseases - May 19, 2022 Category: Parasitology Source Type: research
Molecular typing of the actin gene of Trichomonas vaginalis isolates in Tehran, Iran
This study evaluated actin genotypes ofT. vaginalis isolates using PCR –RFLP and sequence analysis in Tehran, Iran. Overall, 850 vaginal samples were collected from women admitted to hospitals affiliated with the Iran University of Medical Sciences in Tehran from 2020-to 2021. The samples were examined by wet mount and cultured. The parasites were harvested, and PCR– RFLP was performed using three endonuclease enzymes of HindII, MseI, and RsaI on allT. vaginalis isolates. Digestion patterns were then compared, and the genotype of these isolates was defined. The PCR products were sequenced. Overall, 12 (1.4%) isolates o...
Source: Journal of Parasitic Diseases - May 17, 2022 Category: Parasitology Source Type: research
Highly sensitive nested polymerase chain reaction to improve the detection of Leishmania species in clinical specimens
AbstractCutaneous leishmaniasis (CL) is one of the most neglected tropical diseases and an important health problem in many countries. It is an endemic disease in most regions of Iraq, while being non-endemic in the Kurdistan Region. The techniques frequently used for detection of CL are not very sensitive. Therefore, this study aimed to identify a sensitive method for diagnosis of CL in clinical samples. The present study was performed in December 2019 to December 2020 in Kalar General Hospital. Clinical samples were collected from 85 suspected CL cases. Sixty-four (75.29%), 71 (83.53%) and 84 (98.82%) cases were detected...
Source: Journal of Parasitic Diseases - May 13, 2022 Category: Parasitology Source Type: research
Molecular identification of the trematode P. ichunensis stat. n. from lungs of siberian tigers justified reappraisal of Paragonimus westermani species complex
AbstractFlukes from the genusParagonimus Braun, 1899 are medically important foodborne trematodes predominantly occurring throughout Asian countries. Providing molecular genetic characteristics based on ITS2 and partial 28 S rDNA of the paragonimids from the Russian Far East, Northeast, South, and Southeast Asian countries, we performed a partial reappraisal ofParagonimus westermani species complex. Members of this complex are genetically distinct worms with different divergence times and explosive expansion during Miocene-Pliocene epochs. We confirm the taxonomic status as valid species forP. ichunensis stat. n. (from t...
Source: Journal of Parasitic Diseases - May 13, 2022 Category: Parasitology Source Type: research
Assessment of Plasmodium falciparum histidine rich protein 2 and /3 (pfhrp 2 & amp;/ pfhrp 3) gene deletion or mutation in Plasmodium falciparum positive blood samples in a tertiary care centre in South India
This study was conducted for a period of 2 years in a tertiary care centre in South India. Blood samples that are microscopically confirmed asP. falciparum but negative by RDT were assessed for the presence ofpfhrp2,pfhrp3, and their flanking genes using conventional PCR. Follow up of the clinical outcomes were also done for these patients. Of the 63 positive samples collected (50 /63) 79.4% wereP.vivax and (13/63) 20.6% wereP.falciparum by PCR. Among the 13P. falciparum positive samples, 4 samples (4/13), (95% CI -10.36% to 61.11%) were found to be RDT negative but microscopically positive.Pfhrp2,pfhrp3 and their flanki...
Source: Journal of Parasitic Diseases - May 11, 2022 Category: Parasitology Source Type: research
Enterobiasis among Yemeni children: a cross-sectional study
AbstractEnterobiasis is a highly infectious parasitic disease spreading worldwide including Yemen. Therefore, this study aimed to investigate the prevalence and risk factors of enterobiasis among Yemeni children in Dhamar governorate. A total of 402 children (aged 1 –12 years) participated in this cross-sectional survey carried out at Thamar University Al-Wahdah Teaching Hospital (TUWTH), between 2018 and 2019.Enterobius vermicularis infection was examined using the adhesive tape technique. Association between risk factors and enterobiasis were measured by prevalence ratio (PR). The overall prevalence of enterobiasis wa...
Source: Journal of Parasitic Diseases - April 28, 2022 Category: Parasitology Source Type: research
Development of a salivary IgA detection method for accurate diagnosis of amebiasis
AbstractAn amebiasis detection method was developed based on identifying anti-Entamoeba histolytica IgA in the saliva of infected individuals. The enzyme-linked immunosorbent assay (ELISA)-based detection method was tested along with microscopy and polymerase chain reaction (PCR) on saliva and stool samples from 110 asymptomatic individuals visiting the Manila Health Department − Public Health Laboratory of the City of Manila, Philippines. A receiver operating curve (ROC) was constructed to compare the ELISA results with PCR results.E. histolytica infection was detected in 18 of the 110 individuals. The developed me...
Source: Journal of Parasitic Diseases - April 26, 2022 Category: Parasitology Source Type: research
Assessment of therapeutic potential of Allium sativum and Zingiber officinale commercial supplements in experimental giardiasis models
In conclusion,A. sativum andZ. officinale preparations showed higher anti-giardial activity compared to MTZ, with higher reduction inGiardia cyst numbers, viability and trophozoite numbers in the experimentally infected hamsters. Further in vivo trials are recommended usingA. sativum andZ. officinale preparations in increasing doses to reach a higher cure rate. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - April 25, 2022 Category: Parasitology Source Type: research
Occurrence of infestation with the isopod, Alitropus typus M. Edwards (Crustacea: Flabellifera: Aegidae) on commercially important freshwater fishes of Kerala, India
AbstractRapidly rising temperatures and increasing organic load in the inland and coastal waters has led to a significant increase in parasite population. The isopodAlitropus typus infestation on fish in these waters have become more frequent, causing mortalities in both wild and cultured fishes. The present study was aimed to investigate the infestation on different fish hosts, mean intensity, prevalence, environmental influences on the parasite abundance and the histopathological changes it causes in the host. A total of 219 isopod specimens were collected from 149 infested fishes in two districts of Kerala, India. Among...
Source: Journal of Parasitic Diseases - April 24, 2022 Category: Parasitology Source Type: research
Induction of apoptosis by hydroalcoholic and methanolic extract of Deracocephalum kotschyi on Trichomonas vaginalis
According to this study,D. kotschyi extracts induce programmed death inT. vaginalis. The extracts ofD. kotschyi can be used to develop antitrichomonal drugs. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - April 23, 2022 Category: Parasitology Source Type: research
Glugea sp. infecting Sardinella aurita in Algeria
AbstractParasitological examination of the commercially important pelagic fishSardinella aurita Valenciennes, 1847 (Clupeidae) from the Eastern coast of Algeria revealed xenomas in the peritoneal cavity, suggesting a microsporidian infection. The prevalence of the disease was approximately 30% on average, higher in smaller individuals and showing significant seasonal variation. The xenomas contained numerous ellipsoidal spores, surrounded by a dense layer of connective tissue. Spore sizes were 6.10 ± 0.38 µm length and 3.54 ± 0.43 µm width. Ultrastructural examination by transmission electron microscopy show...
Source: Journal of Parasitic Diseases - April 13, 2022 Category: Parasitology Source Type: research
