First report on molecular characterization of Oestrus ovis in sheep from India

The objective of the present study is to characterize the dipteran larvae species infesting the sheep being maintained at SRRC, Mannavanur, by means of COI gene based PCR. During the last week of May 2021, post mortem examination of the skull of an Avikalin male sheep (20  months old) revealed the presence of larvae in its nasal sinuses. The larvae were washed in PBS (pH 7.2) and preserved in 70% alcohol. Total genomic DNA was isolated from the larvae using an initial step of grinding with liquid Nitrogen in a sterile mortar and pestle. Using the isolated genomic DN A from the larvae as a template, Cytochrome c oxidase subunit I (COI) gene based PCR was employed using the primers designed based on the COI gene of reference isolate ofOestrus ovis available in the GenBank. Full length COI gene (1534  bp) gene ofOestrus ovis in sheep from South India was targeted in the PCR experiment. The pTZ57R/T vector was used for the cloning of the PCR amplified fragment and the confirmed recombinant plasmid was subjected to sequencing experiments. In addition to morphological examination, based on COI gene based PCR, eventual sequencing experiments and BLAST analysis, it was confirmed that the larvae in the nasal sinuses of sheep from South India wereOestrus ovis. The South Indian isolate ofOestrus ovis is sharing 100% sequence identity both at nucleotide and amino acid levels with that ofO. ovis from Spain. The North Indian isolate ofO. ovis (from Jammu) exhibited 92% and 99% identity a...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research