Anti-helminthic effect of Punica granatum peel extract on Trichinella spiralis worms and muscle larvae: in vitro and in vivo studies
This study aims to investigate the in vivo and in vitro effects ofPunica granatum peels extract (PGPE) on intestinal and muscle phases ofT. spiralis. The adult worms and larvae were isolated and cultured with different concentrations of PGPE ranging from 6.75 to 100 µg/ml and measuring the survival rate was done after 1, 3, 18, 24 and 48 h of incubation, followed by scanning electron microscopic (SEM) examination of isolated parasites. For the in vivo experiment, the infected animals were divided into two main groups: intestinal phase group and muscular pha se group, each group was subdivided into; infected not treated...
Source: Journal of Parasitic Diseases - April 25, 2023 Category: Parasitology Source Type: research
Molecular characterization and phylogenetic analysis of Linguatula serrata isolated from camels, sheep and goats in Iran
The objective of the present study was to investigate the molecular characterization and phylogenetic analysis of nymphal stage ofL. serrata from camels, goats and sheep in Iran. The mesenteric lymph nodes were collected from various ruminants including goats, sheep and camels at Isfahan and Shiraz slaughterhouses and the nymphs were identified using morphological characteristics. After DNA extraction, the 18 S rRNA and Cox1 genes were amplified by polymerase chain reaction. The sequencing of the genes was conducted using specific primers and a capillary DNA analyzer. The comparison of amplified sequences with existing d...
Source: Journal of Parasitic Diseases - April 19, 2023 Category: Parasitology Source Type: research
Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis
In conclusion, NMB-ELISA is a promising sensitive tool for early and specific diagnosis of acute trichinellosis. The use of NMB-LAT could be a helpful screening procedure in field surveys. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - April 16, 2023 Category: Parasitology Source Type: research
Epidemiological analysis of cattle ticks and tick-borne pathogens in Gadag district, Karnataka state in India
AbstractIn the present study, epidemiological analysis on 839 ticks collected from 50 cattle in Gadag district, Karnataka state, tick identification and detection of tick-borne pathogens was conducted by PCR, sequencing, and phylogeny. The morphological identification revealed thatHaemaphysalis spp. [48.6%],Rhipicephalus spp. [48.4%], andHyalomma spp. [3.0%] tick genera in Gadag district. Further, a higher infestation ofHaemaphysalis spp. [69.0%] andRhipicephalus spp. [62.3%] in Shirahatti and Gadag taluk, respectively was observed. Based on the taluk-wise and tick genus-wise analysis, a higher number of ticks was present ...
Source: Journal of Parasitic Diseases - April 13, 2023 Category: Parasitology Source Type: research
Molecular characterization of Amblyomma geoemydae using CO1 mitochondrial gene to validate phenotypic taxonomical evaluation
AbstractAnimal ectoparasites are linked to the spread of serious medical and veterinary important pathogens. Our research intends to close the knowledge gap concerning the numerous ectoparasites that inhabit animals in Wayanad. Ectoparasites in animals brought to the veterinary dispensaries in Wayanad were retrieved and identified morphologically and molecularly. Using a high-quality stereomicroscope, the taxonomic features of the four following species were examined and identified: Haemaphysalis bispinosa,Rhipicephalus annulatus, R. microplus, andAmblyomma geoemydae. The important disease vectorA. geoemydae was reported f...
Source: Journal of Parasitic Diseases - April 11, 2023 Category: Parasitology Source Type: research
A case of chronic schistosomiasis in a Dongola stallion (Equus ferus caballus Linnaeus, 1758) from Nigeria
AbstractSchistosomiasis is a worldwide snail-borne parasitic infestation of man and animals with acute or chronic phases having devastating sequelae. The present case report focused on post-mortem examination of a cachexic Dongola stallion (Equus ferus caballus Linnaeus, 1758) in Abuja, Nigeria, that failed to respond to treatment. Typical dense collagenous granulomatous lesions with marked inflammatory responses and fibrosis occurred in the liver and several visceral organs of the horse along with other lesions indicative of systemic collapse. Special Ziehl –Neelsen and Periodic Acid-Schiff staining as well as microbial...
Source: Journal of Parasitic Diseases - April 8, 2023 Category: Parasitology Source Type: research
Nasopharyngeal myiasis due to Cephalopina titillator in Southeastern Iran: a prevalence, histopathological, and molecular assessment
This study aimed to explore the prevalence, histopathological effects, and molecular identification ofC. titillator infestation in camels of Kerman province, South-Eastern Iran, between 2019 and 2021. The larvae were placed in 10% formalin for histopathological evaluation and species identification. Pieces of larval abdominal segments ofC. titillator were selected for extraction of DNA. Partial mitochondrial CO1 genes were sequenced for final analysis. Out of the 870 camels examined, 339 (38.9%) were infested with larval stages ofC. titillator. There was a significant difference between age and infection rate (P = 0.00...
Source: Journal of Parasitic Diseases - April 6, 2023 Category: Parasitology Source Type: research
The in vitro and in vivo effects of Lucilia sericata larval secretions on Leishmania major
This study suggested the pla usible inhibitory effects ofL. sericata larvae ’s secretions on theL. major amastigotes and CL lesions progression. It seems that the characterization of all effective components/proteins in the larvae secretions and their specific targets in parasite structure or in cell (macrophage) responses could further reveal more details regarding the anti-leishmanial properties of these compounds. (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - April 5, 2023 Category: Parasitology Source Type: research
The potential curative and hepatoprotective effects of platelet rich plasma on liver fibrosis in Schistosoma mansoni experimentally infected mice
AbstractTrappedSchistosoma mansoni eggs trigger fibrotic liver disease that can continue to liver cirrhosis and failure. This work evaluates the outcome of platelet rich plasma (PRP) onS. mansoni-induced liver fibrosis by intraperitoneal (IP) and intrahepatic (IH) routes with/without Praziquantel (PZQ) treatment. Swiss albino mice (n = 162) were divided into non-infected (n = 66) and infected (n = 96) groups, then subdivided into non-treated and treated subgroups with PRP(IP), PRP(IH) 6th and 10th weeks post-infection, PZQ, PZQ + PRP(IP) and PZQ + PRP(IH) 6th and 10th weeks post-infection. Effects of tr...
Source: Journal of Parasitic Diseases - April 3, 2023 Category: Parasitology Source Type: research
Gastrointestinal parasites of zoonotic importance detected in porcine faeces in Chitwan National Park, Nepal
This study has provided baseline information regarding the diversity of gastrointestinal parasites in wild boars. It requires continuous study at molecular level to explore other species of parasites and verify their zoonotic potential.Graphical abstract (Source: Journal of Parasitic Diseases)
Source: Journal of Parasitic Diseases - March 31, 2023 Category: Parasitology Source Type: research
Analysis of antioxidant enzymes and oxidative stress markers in the liver of naturally infected Indian water buffalo (Bubalus bubalis) with cystic echinococcosis
AbstractThe present study assessed the antioxidants and oxidative stress markers in the liver of buffalo naturally infected with cystic echinococcosis. Infected and non-infected livers were collected from the abattoir, and processed to determine the markers of oxidative stress and antioxidants. In addition, samples were also analyzed for liver tissue injury markers. A significantly higher level of glutathione-s-transferase (GST) and glutathione peroxidase (GPx) were observed in the infected liver compared to a healthy liver. On the other hand, the levels of glutathione reductase (GR) and thioredoxin reductase (TR) were sig...
Source: Journal of Parasitic Diseases - March 30, 2023 Category: Parasitology Source Type: research
Morphological and molecular detection of myxosporean parasites in freshwater fish in Myanmar
In this study, morphological study revealed that fiveMyxobolus spp. (Myxobolus sp. 1, 4 –6, and 9) and twoThelohanellus spp. (Thelohanellus sp. 1 and 2) were found to be infected in the gills (gill filaments) and kidneys, fourMyxobolus spp. (Myxobolus sp. 2, 3, 7 and 8) were infected in the gills and oneMyxobolus sp. (Myxobolus sp. 10) was infected in the kidneys of four studied fish species. Among them, three sequences (LC510617, LC510618 and LC510619) were isolated from detected parasites. The obtained sequences were similar (88.1 –98.8%) to those derived from myxosporean parasites deposited in GenBank. This is the f...
Source: Journal of Parasitic Diseases - March 29, 2023 Category: Parasitology Source Type: research
Myxozoan fish diseases: possible treatment and zoonoses
AbstractMyxozoan parasites pose huge threat to wild and cultured fishes and reported to cause heavy mortality, retarded growth and post-harvest quality degradation. It is one of the highly divergent groups of parasites which infects skin, gill, muscle, cartilage and internal organs of host fish and the severity of pathology varies depending on the water temperature, species of fish, site of tissue infection and immune resistance of the individual host. Most infections are difficult to treat, as they can easily evade host cellular and humoral defence mechanisms by proliferating or migrating through immune compromised sites ...
Source: Journal of Parasitic Diseases - March 27, 2023 Category: Parasitology Source Type: research
The potential therapeutic effect of Nigella sativa and Zingiber officinale extracts versus Nitazoxanide drug against experimentally induced cryptosporidiosis in laboratory mice
In this study, the potential anti-cryptosporidial effect ofNigella sativa (black seeds) andZingiber officinale (ginger) alcoholic extracts versus Nitazoxanide (NTZ) medication was investigated in immunosuppressed (IS) laboratory mice. Parasitological, histopathological studies were used to assess their therapeutic efficacy. Serum level and tissue expression percentage of IFN- γ was also used. Nigella extract succeeded to reduce the mean oocyst counts in the feces of immunosuppressed mice followed by NTZ. Ginger-treated ones showed the lowest reduction percentage.Nigella sativa showed the best results in terms of restoring...
Source: Journal of Parasitic Diseases - March 25, 2023 Category: Parasitology Source Type: research
Type-1 diabetes mellitus down-regulated local cerebral glial fibrillary acidic protein expression in experimental toxoplasmosis
AbstractCerebral toxoplasmosis is an opportunistic infection, occurring mostly in immunosuppressed patients due to the reactivation of latentToxoplasma cysts. The cerebral comorbidity in diabetic patients tends to intensify the burden of pathogenic infection within the brain. The aim of this work was to study the effect of cerebral toxoplasmosis in experimentally infected hyperglycemic mice, on histopathology and glial fibrillary acidic protein (GFAP) expression, compared to normoglycemic mice at different time intervals. Vasculopathy was exclusively observed in diabetic groups, with features of increased severity duringTo...
Source: Journal of Parasitic Diseases - March 18, 2023 Category: Parasitology Source Type: research
