Multiple barriers as an efficient treatment for removing pesticides aiming Direct Potable Reuse: a pilot scale study
In this study, the pesticides and degradation products, atrazine (ATZ), hydroxyatrazine (ATZOH), deethylatrazine (DEA), deisopropylatrazine (DIA), simazine (SMZ), ametryn (AMT), diuron (DIU), 2,4-D, fipronil (FIP), fipronil sulfide (FIP-SF) and fipronil sulfone (FIP-SN) were evaluated in effluent after membrane bioreactor (MBR), effluent after advanced treatment by multiple barriers (MBR, reverse osmosis, UV/H2O2 and activated carbon), in tap water collected in the urban region of Campinas and in the Atibaia River (water supply source from city of Campinas). The pesticide concentrations in the Atibaia River and the post-MB...
Source: Environmental Pollution - April 26, 2024 Category: Environmental Health Authors: J éssyca Ferreira de Medeiros Cassiana Carolina Montagner Source Type: research

An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis
Conclusions: A high correlation of the QIAstat-Dx ME panel with reference methods was shown. QIAstat-Dx ME is a rapid-PCR technique to be applied in patients with suspected ME with a high accuracy.PMID:38667448 | DOI:10.3390/diagnostics14080802 (Source: Herpes)
Source: Herpes - April 26, 2024 Category: Infectious Diseases Authors: Genoveva Cuesta Pedro Puerta-Alcalde Andrea Vergara Enric Roses Jordi Bosch Climent Casals-Pascual Alex Soriano M ª Ángeles Marcos Sergi Sanz Jordi Vila Source Type: research

Microbiological assessment of success and failure in pulp revitalization: a randomized clinical trial using calcium hydroxide and chlorhexidine gluconate in traumatized immature necrotic teeth
CONCLUSIONS: There were no statistical differences in CH and CHD efficacy. At S5, microflora persisted in both successful and failed outcomes, but the abundance and diversity increased significantly only in the failed cases. The successful outcomes presented higher diversity and higher decreases of the primary microflora at S5 than the failed outcomes. The abundance and diversity increased significantly at S5 only in failed cases.PMID:38665416 | PMC:PMC11044761 | DOI:10.1080/20002297.2024.2343518 (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - April 26, 2024 Category: Microbiology Authors: Alina Wikstr öm Nelly Romani Vestman Olena Rakhimova David Lazaro Gimeno Georgios Tsilingaridis Malin Brundin Source Type: research

Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease
Volume 16, Issue 1, January-December 2024 . (Source: Gut Microbes)
Source: Gut Microbes - April 26, 2024 Category: Microbiology Authors: Chinasa Valerie OlovoDickson Kofi Wiredu OcanseyYing JiXinxiang HuangMin Xua Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, Chinab Department of Biochemistry and Molecular Biology, School of Medicine, Jiangs Source Type: research

Seroprevalence and molecular detection of Toxoplasma gondii and Neospora caninum in beef cattle and goats in Hunan province, China
Toxoplasma gondii and Neospora caninum are closely related protozoan parasites that are considered important causes of abortion in livestock, causing huge economic losses. Hunan Province ranks 12th in the product... (Source: Parasites and Vectors)
Source: Parasites and Vectors - April 26, 2024 Category: Microbiology Authors: Xi-Long Yi, Wen-He Yang, He-Liang Zheng, Mei-Ling Cao, Jun Xiong, Wen-Cheng Chen, Yu-Jing Zhou, Fen Li, Xing-Quan Zhu and Guo-Hua Liu Tags: Research Source Type: research

Methionine inducing carbohydrate esterase secretion of Trichoderma harzianum enhances the accessibility of substrate glycosidic bonds
The conversion of plant biomass into biochemicals is a promising way to alleviate energy shortage, which depends on efficient microbial saccharification and cellular metabolism. Trichoderma spp. have plentiful CA... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 26, 2024 Category: Microbiology Authors: Yang Liu, Tuo Li, Han Zhu, Linhua Cao, Lebin Liang, Dongyang Liu and Qirong Shen Tags: Research Source Type: research

Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease
Volume 16, Issue 1, January-December 2024 . (Source: Gut Microbes)
Source: Gut Microbes - April 26, 2024 Category: Microbiology Authors: Chinasa Valerie OlovoDickson Kofi Wiredu OcanseyYing JiXinxiang HuangMin Xua Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, Chinab Department of Biochemistry and Molecular Biology, School of Medicine, Jiangs Source Type: research

Histone H2B lysine 122 and lysine 130, as the putative targets of Penicillium oxalicum LaeA, play important roles in asexual development, expression of secondary metabolite gene clusters, and extracellular glycoside hydrolase synthesis
AbstractCore histones in the nucleosome are subject to a wide variety of posttranslational modifications (PTMs), such as methylation, phosphorylation, ubiquitylation, and acetylation, all of which are crucial in shaping the structure of the chromatin and the expression of the target genes. A putative histone methyltransferase LaeA/Lae1, which is conserved in numerous filamentous fungi, functions as a global regulator of fungal growth, virulence, secondary metabolite formation, and the production of extracellular glycoside hydrolases (GHs). LaeA ’s direct histone targets, however, were not yet recognized. Previous researc...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research

High-level production of chitinase by multi-strategy combination optimization in Bacillus licheniformis
The objective of this study was to utilize the extracellular chitinase fromBacillus thuringiensis as the target, andBacillus licheniformis as the expression host to achieve heterologous expression of ChiA with enhanced activity. Initially, through structural analysis and molecular dynamics simulation, we identified key amino acids to improve the enzymatic performance of chitinase, and the specific activity of chitinase mutant D116N/E118N was 48% higher than that of the natural enzyme, with concomitant enhancements in thermostability and pH stability. Subsequently, the expression elements of ChiA(D116N/E118N) were screened ...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research

Effect of Ba2+ on the biomineralization of Ca2+ and Mg2+ ions induced by Bacillus licheniformis
In this study,Bacillus licheniformis was used to bio-precipitate calcium, magnesium, and barium ions. The effects of barium ions on the physiological and biochemical characteristics of bacteria, as well as the components of extracellular polymers and mineral characteristics, were also studied in systems containing coexisting barium, calcium, and magnesium ions. The results show that the increasing concentrations of barium ions decreased pH, carbonic anhydrase activity, and concentrations of bicarbonate and carbonate ions, while it increased the contents of humic acids, proteins, polysaccharides, and DNA in extracellular po...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research

DNA adduct formation in Saccharomyces cerevisiae following exposure to environmental pollutants, as in vivo model for molecular toxicity studies
AbstractDNA adduction in the model yeastSaccharomyces cerevisiae was investigated after exposure to the fungicide penconazole and the reference genotoxic compound benzo(a)pyrene, for validating yeasts as a tool for molecular toxicity studies, particularly of environmental pollution. The effect of the toxicants on the yeast ’s growth kinetics was determined as an indicator of cytotoxicity. Fermentative cultures ofS. cerevisiae were exposed to 2  ppm of Penconazole during different phases of growth; while 0.2 and 2 ppm of benzo(a)pyrene were applied to the culture medium before inoculation and on exponential cultures. Ex...
Source: World Journal of Microbiology and Biotechnology - April 26, 2024 Category: Microbiology Source Type: research

Oligella spp.: A systematic review on an uncommon urinary pathogen
ConclusionOligella is an uncommon pathogen that can be underrecognized. Microbiologists should be prompt to prolong the incubation of agar media plated with urines when the direct examination showed Gram-negative coccobacilli. Carbapenems should probably be given for the empirical treatment. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 26, 2024 Category: Microbiology Source Type: research

Cutibacterium Acnes in Spine Surgery: Pathophysiology, Diagnosis, and Treatment
Cutibacterium acnes (C. acnes) previously named Propionibacterium acnes (P. acnes) has been increasingly recognized by spine surgeons as a cause of indolent post-surgical spinal infection. Patients infected with C acnes may present with pseudarthrosis or nonspecific back pain. Currently, microbiological tissue cultures remain the gold standard in diagnosing C. acnes infection. Ongoing research into using genetic sequencing as a diagnostic method shows promising results and may be another future way of diagnosis. (Source: The Spine Journal)
Source: The Spine Journal - April 26, 2024 Category: Orthopaedics Authors: Makeen Baroudi, Mohammad Daher, Russell D Parks, Jerzy George Gregoryczyk, Mariah Balmaceno-Criss, Christopher L McDonald, Bassel G Diebo, Alan H Daniels Source Type: research

Knowledge, attitudes and perceptions of Latin American healthcare workers relating to antibiotic stewardship and antibiotic use: a cross-sectional multi-country study
ConclusionsAlthough HCWs perceived improving AU as a priority, they did not perceive AU or AMR as a problem in their HCF. AS opportunities include improved access to guidelines, access to AMR/AU data, teamwork, and education on AS for HCWs and patients and families. (Source: Antimicrobial Resistance and Infection Control)
Source: Antimicrobial Resistance and Infection Control - April 26, 2024 Category: Infectious Diseases Source Type: research

Evidence and antibiotic resistance profiles of clinical Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) and non-ACB complex members in companion animals: A 2020-2022 retrospective study
Comp Immunol Microbiol Infect Dis. 2024 Apr 17;109:102185. doi: 10.1016/j.cimid.2024.102185. Online ahead of print.ABSTRACTTo evaluate the frequency of Acinetobacter spp., belonging to both Acinetobacter calcoaceticus-baumannii (ACB) and non-ACB complex, and their antibiotic resistance profiles in veterinary medicine, a three-year (2020-2022) retrospective study was carried out on sick companion animals. Epidemiological data from different clinical canine, feline, and equine samples, were acquired. For each strain, MALDI-TOF MS identification and susceptibility to a panel of 11 antibiotics, by Kirby-Bauer and E-test method...
Source: Comparative immunology, microbiology and infectious diseases. - April 25, 2024 Category: Allergy & Immunology Authors: Anna-Rita Attili Francesca Paola Nocera Martina Sisto Martina Linardi Francesca Gigli Victor Ngu Ngwa Filomena Fiorito Claudia Cerracchio Marina C T Meligrana Eleonora Bonacucina Vincenzo Cuteri Luisa De Martino Source Type: research