Efficacy of an oral lipid nanocrystal formulation of amphotericin B (MAT2203) in the neutropenic mouse model of pulmonary mucormycosis
Antimicrob Agents Chemother. 2024 Apr 30:e0154023. doi: 10.1128/aac.01540-23. Online ahead of print.ABSTRACTInvasive mucormycosis (IM) is associated with high mortality and morbidity. MAT2203 is an orally administered lipid nanocrystal formulation of amphotericin B, which has been shown to be safe and effective against other fungal infections. We sought to compare the efficacy of MAT2203 to liposomal amphotericin B (LAMB) treatment in a neutropenic mouse model of IM due to Rhizopus arrhizus var. delemar or Mucor circinelloides f. jenssenii DI15-131. In R. arrhizus var. delemar-infected mice, 15 mg/kg of MAT2203 qd was as e...
Source: Antimicrobial Agents and Chemotherapy - April 30, 2024 Category: Microbiology Authors: Yiyou Gu Teclegiorgis Gebremariam Sondus Alkhazraji Eman Youssef Sabrina El-Gamal Theresa Matkovits Jenel Cobb Raphael Mannino Ashraf S Ibrahim Source Type: research
The nucleoside analog 4'-fluorouridine suppresses the replication of multiple enteroviruses by targeting 3D polymerase
In this study, we found that 4'-fluorouridine (4'-FlU), a nucleoside analog with low cytotoxicity, exhibited broad-spectrum activity against infections of multiple enteroviruses with EC50 values at low micromolar levels, including coxsackievirus A10 (CV-A10), CV-A16, CV-A6, CV-A7, CV-B3, enterovirus A71 (EV-A71), EV-A89, EV-D68, and echovirus 6. With further investigation, the results indicated that 4'-FlU directly interacted with the RNA-dependent RNA polymerase of enterovirus, the 3D pol, and impaired the polymerase activity of 3D pol, hence inhibiting viral RNA synthesis and significantly suppressing viral replication. ...
Source: Antimicrobial Agents and Chemotherapy - April 30, 2024 Category: Microbiology Authors: Yongkang Chen Xiaohong Li Fengyang Han Beihong Ji Yuan Li Jingjing Yan Min Wang Jun Fan Shuye Zhang Lu Lu Peng Zou Source Type: research
The ecto-3'-nucleotidase activity of Acanthamoeba castellanii trophozoites increases their adhesion to host cells through the generation of extracellular adenosine
Eur J Protistol. 2024 Apr 26;94:126086. doi: 10.1016/j.ejop.2024.126086. Online ahead of print.ABSTRACTAcanthamoeba castellanii, a free-living amoeba, can be pathogenic to humans causing a corneal infection named Acanthamoeba keratitis (AK). The mannose-binding protein (MBP) is well established as the major factor related to Acanthamoeba pathogenesis. However, additional factors that participate in the adhesion process and protect trophozoites from cytolytic effects caused by host immune responses remain unknown. Ectonucleotidases, including 3'-nucleotidase/nuclease (3'-NT/NU), a bifunctional enzyme that was recently repor...
Source: European Journal of Protistology - April 30, 2024 Category: Microbiology Authors: Eduarda de Souza-Maciel Luiz Fernando Carvalho-Kelly Anita Leocadio Freitas-Mesquita Jos é Roberto Meyer-Fernandes Source Type: research
Contractile vacuoles: a rapidly expanding (and occasionally diminishing?) understanding
Eur J Protistol. 2024 Mar 26;94:126078. doi: 10.1016/j.ejop.2024.126078. Online ahead of print.ABSTRACTOsmoregulation is the homeostatic mechanism essential for the survival of organisms in hypoosmotic and hyperosmotic conditions. In freshwater or soil dwelling protists this is frequently achieved through the action of an osmoregulatory organelle, the contractile vacuole. This endomembrane organelle responds to the osmotic challenges and compensates by collecting and expelling the excess water to maintain the cellular osmolarity. As compared with other endomembrane organelles, this organelle is underappreciated and under-s...
Source: European Journal of Protistology - April 30, 2024 Category: Microbiology Authors: Kiran J More Harpreet Kaur Alastair G B Simpson Frederick W Spiegel Joel B Dacks Source Type: research
Broadly neutralizing antibodies for HIV prevention: a comprehensive review and future perspectives
Clin Microbiol Rev. 2024 Apr 30:e0015222. doi: 10.1128/cmr.00152-22. Online ahead of print.ABSTRACTSUMMARYThe human immunodeficiency virus (HIV) epidemic remains a formidable global health concern, with 39 million people living with the virus and 1.3 million new infections reported in 2022. Despite anti-retroviral therapy's effectiveness in pre-exposure prophylaxis, its global adoption is limited. Broadly neutralizing antibodies (bNAbs) offer an alternative strategy for HIV prevention through passive immunization. Historically, passive immunization has been efficacious in the treatment of various diseases ranging from onco...
Source: Clinical Microbiology Reviews - April 30, 2024 Category: Microbiology Authors: Sharana Mahomed Source Type: research
< em > Anaerolentibacter hominis < /em > gen. nov. sp. nov., < em > Diplocloster hominis < /em > sp. nov. and < em > Pilosibacter fragilis < /em > gen. nov. sp. nov., isolated from human faeces
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006359.ABSTRACTThree Gram-positive, obligately anaerobic bacterial strains, namely CSJ-1T, CSJ-3T, and CSJ-4T, were isolated from faeces of healthy persons. They were characterized through a combination of whole-genome sequencing, phenotypic traits, and metabolomic analysis. The genome sizes of CSJ-1T, CSJ-4T, and CSJ-3T were 3.3, 3.8, and 6.1 Mbp, with DNA G+C contents of 47.2, 48.3, and 48.8 mol%, respectively. Strain CSJ-3T was identified as representing a novel species, Diplocloster hominis (type strain CSJ-3T=CGMCC 1.18033T=JCM 36512T) of the genus Diploc...
Source: International Journal of Systematic and Evolutionary Microbiology - April 30, 2024 Category: Microbiology Authors: Yang Yan Qing-Hua Zhao Chang Xu Rui-Qi Wei He Jiang Shuang-Jiang Liu Source Type: research
Disease-induced changes in bacterial and fungal communities from plant below- and aboveground compartments
In this study, we investigated the bacterial and fungal communities in bulk soil, rhizosphere soil, root, stem, and leaf of both healthy and infected (Potato virus Y disease, PVY) plants. The results indicated that bacterial and fungal communities showed different recruitment strategies in plant organs. The number and abundance of shared bacterial ASVs between bulk and rhizosphere soils decreased with ascending migration from below- to aboveground compartments, while the number and abundance of fungal ASVs showed no obvious changes. Field type, plant compartments, and PVY infection all affected the diversity and structures...
Source: Applied Microbiology and Biotechnology - April 30, 2024 Category: Microbiology Authors: Mingfeng Cao Songqing Huang Jingjing Li Xiaoming Zhang Yi Zhu Jingzhao Sun Li Zhu Yong Deng Jianqiang Xu Zhihua Zhang Qiang Li Jixiang Ai Tian Xie Hengli Li Huaqun Yin Wuyuan Kong Yabing Gu Source Type: research
Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications
Biotechnol Adv. 2024 Apr 27:108369. doi: 10.1016/j.biotechadv.2024.108369. Online ahead of print.ABSTRACTElectromethanogenesis has emerged as a biological branch of Power-to-X technologies that implements methanogenic microorganisms, as an alternative to chemical Power-to-X, to convert electrical power from renewable sources, and CO2 into methane. Unlike biomethanation processes where CO2 is converted via exogenously added hydrogen, electromethanogenesis occurs in a bioelectrochemical set-up that combines electrodes and microorganisms. Thereby, mixed, or pure methanogenic cultures catalyze the reduction of CO2 to methane v...
Source: Biotechnology Advances - April 30, 2024 Category: Biotechnology Authors: Paola Andrea Palacios Jo Philips Anders Bentien Michael Vedel Wegener Kofoed Source Type: research
Bacterial synthase-dependent exopolysaccharide secretion: a focus on cellulose
Curr Opin Microbiol. 2024 Apr 29;79:102476. doi: 10.1016/j.mib.2024.102476. Online ahead of print.ABSTRACTBacterial biofilms are a prevalent multicellular life form in which individual members can undergo significant functional differentiation and are typically embedded in a complex extracellular matrix of proteinaceous fimbriae, extracellular DNA, and exopolysaccharides (EPS). Bacteria have evolved at least four major mechanisms for EPS biosynthesis, of which the synthase-dependent systems for bacterial cellulose secretion (Bcs) represent not only key biofilm determinants in a wide array of environmental and host-associat...
Source: Current Opinion in Microbiology - April 30, 2024 Category: Microbiology Authors: Petya V Krasteva Source Type: research
Time to switch gears: how long noncoding RNAs function as epigenetic regulators in Apicomplexan parasites
Curr Opin Microbiol. 2024 Apr 29;79:102484. doi: 10.1016/j.mib.2024.102484. Online ahead of print.ABSTRACTLong noncoding RNAs (lncRNA) are emerging as important regulators of gene expression in eukaryotes. In recent years, a large repertoire of lncRNA were discovered in Apicomplexan parasites and were implicated in several mechanisms of gene expression, including marking genes for activation, contributing to the formation of subnuclear compartments and organization, regulating the deposition of epigenetic modifications, influencing chromatin and chromosomal structure and manipulating host gene expression. Here, we aim to u...
Source: Current Opinion in Microbiology - April 30, 2024 Category: Microbiology Authors: Vera Mitesser Karina Simantov Ron Dzikowski Source Type: research
Acidification induce chemical and microbial variation in tea plantation soils and bacterial degradation of the key acidifying phenolic acids
Arch Microbiol. 2024 Apr 30;206(5):239. doi: 10.1007/s00203-024-03858-z.ABSTRACTCamellia sinensis is an important economic plant grown in southern subtropical hilly areas, especially in China, mainly for the production of tea. Soil acidification is a significant cause of the reduction of yield and quality and continuous cropping obstacles in tea plants. Therefore, chemical and microbial properties of tea growing soils were investigated and phenolic acid-degrading bacteria were isolated from a tea plantation. Chemical and ICP-AES investigations showed that the soils tested were acidic, with pH values of 4.05-5.08, and the p...
Source: Archives of Microbiology - April 30, 2024 Category: Microbiology Authors: Yuhan Zhang Binjie Wang Guiwei Wang Zhisheng Zheng Ying Chen Ou Li Yulong Peng Xiufang Hu Source Type: research
Reclassification of Aestuariicella albida as Pseudomaricurvus albidus comb. nov. and Aestuariicella hydrocarbonica as Pseudomaricurvus hydrocarbonicus comb. nov. Based on Comparative Genomics and Molecular Synapomorphies
Curr Microbiol. 2024 Apr 30;81(6):159. doi: 10.1007/s00284-024-03683-z.ABSTRACTThe genus Aestuariicella has been recently reclassified as a member of the family Cellvibrionaceae. However, the taxonomic position of the genus as a distinct member of the family has not been clarified. In the present study, we performed multilayered analyses anchored on genome sequences to clarify the relationship between the genera Aestuariicella and Pseudomaricurvus within the family Cellvibrionaceae. Phylogenetic analyses based on 16S rRNA gene, RNA polymerase beta subunit (RpoB) protein, and core gene sequences showed a well-supported tigh...
Source: Current Microbiology - April 30, 2024 Category: Microbiology Authors: Jake Adolf V Montecillo Merell P Billacura Maria Distressa G Billacura Source Type: research
Anti-mildew and fresh-keeping effect of Lactiplantibacillus paraplantarum P3 cell-free supernatant on fresh in-shell peanuts during storage process
This study provides research data for the application of L. paraplantarum P3 CFS in the preservation and antimildew of fresh in-shell peanuts.PMID:38688186 | DOI:10.1016/j.ijfoodmicro.2024.110719 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - April 30, 2024 Category: Food Science Authors: Haoxin Lv Chaoyue Cui Zubin Wang Yijun Liu Shichang Liu Tianjie Qi Yanfei Li Yan Zhao Source Type: research
Development of rabbit models of ventilator-associated bacterial pneumonia produced by carbapenem-resistant < em > Pseudomonas aeruginosa < /em >
Antimicrob Agents Chemother. 2024 Apr 30:e0020524. doi: 10.1128/aac.00205-24. Online ahead of print.ABSTRACTVentilator-associated bacterial pneumonia (VABP) is among the most intractable of carbapenem-resistant Gram-negative bacterial infections. New antimicrobial agents are critically needed for the treatment of VABP. However, current conventionally used animal model systems are inadequate to meet this challenge. We, therefore, developed rabbit models of VABP caused by carbapenem-resistant Pseudomonas aeruginosa. Persistently neutropenic New Zealand White rabbits were used throughout the study. The early-phase intubated m...
Source: Antimicrobial Agents and Chemotherapy - April 30, 2024 Category: Microbiology Authors: Vidmantas Petraitis Ruta Petraitiene Povilas Kavaliauskas Ethan Naing Andrew Garcia Vilma Zigmantaite Ramune Grigaleviciute Audrius Kucinskas Alius Pockevicius Rimantas Stakauskas Thomas J Walsh Source Type: research
Delayed infections following polybutylate-coated polyester (Ethibond) suture frontalis suspension surgery for severe blepharoptosis
CONCLUSION: Frontalis suspension with Ethibond has an 8% incidence of delayed infections, with Staphylococcus aureus as the most common organism. The authors recommend early sling removal in all patients with infection and recommend consideration of an alternative material in the event of future revision surgery.PMID:38687955 | DOI:10.1080/01676830.2024.2338789 (Source: Orbit)
Source: Orbit - April 30, 2024 Category: Opthalmology Authors: Ayushi Agarwal Joveeta Joseph Milind N Naik Source Type: research
