Update on the classification of higher ranks in the phylum Actinobacteria.
Abstract Genome analysis is one of the main criteria for description of new taxa. Availability of genome sequences for all the actinobacteria with a valid nomenclature will, however, require another decade's works of sequencing. This paper describes the rearrangement of the higher taxonomic ranks of the members of the phylum 'Actinobacteria', using the phylogeny of 16S rRNA gene sequences and supported by the phylogeny of the available genome sequences. Based on the refined phylogeny of the 16S rRNA gene sequences, we could arrange all the members of the 425 genera of the phylum 'Actinobacteria' with validly publi...
Source: International Journal of Systematic and Evolutionary Microbiology - December 6, 2019 Category: Microbiology Authors: Salam N, Jiao JY, Zhang XT, Li WJ Tags: Int J Syst Evol Microbiol Source Type: research

Glycomyces buryatensis sp. nov., an actinobacterium isolated from steppe soil.
Abstract A novel actinobacterial strain, designated 18T, was isolated from a steppe soil sample collected in Buryatia, Russia and subjected to polyphasic taxonomic characterization. The strain was aerobic and Gram-stain-positive. The 16S rRNA gene sequence of strain 18T exhibited highest similarity to Glycomyces paridis CPCC 204357T (97.2 %). Results of phylogenetic analysis showed that strain 18T formed a distinct branch clearly affiliated to the genus Glycomyces. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the cell-wall diamino acid. The whole-cell sugar profile was found to cont...
Source: International Journal of Systematic and Evolutionary Microbiology - December 6, 2019 Category: Microbiology Authors: Nikitina E, Liu SW, Li FN, Buyantueva L, Abidueva E, Sun CH Tags: Int J Syst Evol Microbiol Source Type: research

Anaerohalosphaera lusitana gen. nov., sp. nov., and Limihaloglobus sulfuriphilus gen. nov., sp. nov., isolated from solar saltern sediments, and proposal of Anaerohalosphaeraceae fam. nov. within the order Sedimentisphaerales.
Abstract Two strains of anaerobic, coccoid, saccharolytic, Gram-stain-negative bacteria were isolated from samples of anoxic hypersaline sediments of evaporation ponds in Tavira (Portugal) and Mallorca (Spain). Both isolates were moderately halophilic, neutrophilic and had a temperature optimum at 37 °C. The highest 16S rRNA gene sequence identity values were found with members of the genus Sedimentisphaera (84.9-88.2 %) within the order Sedimentisphaerales, class Phycisphaerae. The strain SM-Chi-D1T could be assigned to the family Sedimentisphaeraceae, while phylogenetic analyses based on 16S rRNA ge...
Source: International Journal of Systematic and Evolutionary Microbiology - December 5, 2019 Category: Microbiology Authors: Pradel N, Fardeau ML, Tindall BJ, Spring S Tags: Int J Syst Evol Microbiol Source Type: research

Novosphingobium umbonatum sp. nov., isolated from a freshwater mesocosm.
Abstract A bacterial strain designated FSY-9T was isolated from a freshwater mesocosm in Taiwan and characterized to determine its taxonomic affiliation. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-9T formed a phylogenetic lineage in the genus Novosphingobium. Strain FSY-9T was most closely related to Novosphingobium humi R1-4T with a 97.2 % 16S rRNA gene sequence similarity. Strain FSY-9T showed 71.3-72.6 % average nucleotide identity and 17.7-23.0 % digital DNA-DNA hybridization identity with the strains of other...
Source: International Journal of Systematic and Evolutionary Microbiology - December 5, 2019 Category: Microbiology Authors: Sheu SY, Cai CY, Kwon SW, Chen WM Tags: Int J Syst Evol Microbiol Source Type: research

Elioraea thermophila sp. nov., a thermophilic bacterium from hot spring of the class Alphaproteobacteria, emended description of the genus Elioraea and proposal of Elioraeaceae fam. nov.
Abstract A Gram-staining-negative, aerobic, curved rod-shaped and thermophilic bacterial strain, designated YIM 72297T, was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China. Growth was observed at pH 5.0-9.0 with an optimum of pH 7.0-7.5, and at 45-60 °C with an optimum of 55 °C. Positive for catalase and oxidase. The 16S rRNA gene sequence comparison indicated that strain YIM 72297T was most closely related to Elioraea tepidiphila DSM 17972T (96.9 %) and showed 5 %) were summed C18 : 0 (35.8 %), summed feature 8 (30....
Source: International Journal of Systematic and Evolutionary Microbiology - December 5, 2019 Category: Microbiology Authors: Habib N, Khan IU, Xiao M, Hejazi MS, Tarhriz V, Zhi XY, Li WJ Tags: Int J Syst Evol Microbiol Source Type: research

Rhodobacter sediminicola sp. nov., isolated from a fresh water pond.
Abstract A phototrophic bacterium, designated as strain JA983T, was isolated from a freshwater pond in Gujarat, India. The strain was yellowish brown, catalase- and oxidase-positive, rod-to-oval shaped, Gram-stain-negative and motile. Growth was observed at 20-35 °C. NaCl was not required for optimum growth and up to 5 % was tolerated. Growth was observed at pH 6.0-8.0, with an optimum at pH 7.0. An unidentified glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminolipids (AL1, AL2) and two unidentified lipids (L1 and L2) are the...
Source: International Journal of Systematic and Evolutionary Microbiology - December 5, 2019 Category: Microbiology Authors: G S, Kumar D, A K, Ch S, Ch V R Tags: Int J Syst Evol Microbiol Source Type: research

Limnoglobus roseus gen. nov., sp. nov., a novel freshwater planctomycete with a giant genome from the family Gemmataceae.
sh SN Abstract The family Gemmataceae accommodates aerobic, chemoorganotrophic planctomycetes, which inhabit various freshwater ecosystems, wetlands and soils. Here, we describe a novel member of this family, strain PX52T, which was isolated from a boreal eutrophic lake in Northern Russia. This isolate formed pink-pigmented colonies and was represented by spherical cells that occurred singly, in pairs or aggregates and multiplied by budding. Daughter cells were highly motile. PX52T was an obligate aerobic chemoorganotroph, which utilized various sugars and some heteropolysaccharides. Growth occurred at pH 5.0-7.5 ...
Source: International Journal of Systematic and Evolutionary Microbiology - December 4, 2019 Category: Microbiology Authors: Kulichevskaya IS, Naumoff DG, Miroshnikov KK, Ivanova AA, Philippov DA, Hakobyan A, Rijpstra WIC, Damsté JSS, Liesack W, Dedysh SN Tags: Int J Syst Evol Microbiol Source Type: research

Cumulibacter soli sp. nov., isolated from farmland soil.
Abstract A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterium, designated as strain G-1T, was isolated from farmland soil sampled in in Fuyang, Anhui Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G-1T was closely related to Cumulibacter manganitolerans 2-36T (97.7 % similarity). Strain G-1T contained iso-C16 : 0, C17 : 1ω6c, iso-C15 : 0 and iso-C14 : 0 as the predominant fatty acids. The polar lipids of strain G-1T were diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an...
Source: International Journal of Systematic and Evolutionary Microbiology - December 4, 2019 Category: Microbiology Authors: Gao QQ, Jiang WK, Sun GJ, Zhang L, Zhang ML, Liu XA, Ke ZJ, Zhou YD, Hong Q, Xu JH Tags: Int J Syst Evol Microbiol Source Type: research

Segnochrobactrum spirostomi gen. nov., sp. nov., isolated from the ciliate Spirostomum yagiui and description of a novel family, Segnochrobactraceae fam. nov. within the order Rhizobiales of the class Alphaproteobacteria.
Abstract A bacterial strain, designated Sp-1T, was isolated from the heterotrich ciliate Spirostomum yagiui collected from a reservoir located in Ulsan, Republic of Korea. Cells of Sp-1T were Gram stain-negative, rod-shaped, non-spore-forming, non-motile and contained poly-β-hydroxybutyrate granules. Phylogenetic analyses based on 16S rRNA gene sequences indicated that Sp-1T constituted a distinct phylogenetic lineage within different families in the order Rhizobiales with a pairwise sequence similarity of 95 % to the species of the genus Ochrobactrum: Ochrobactrum anthropi ATCC 49188T and Ochrobactrum cyti...
Source: International Journal of Systematic and Evolutionary Microbiology - December 4, 2019 Category: Microbiology Authors: Akter S, Shazib SUA, Shin MK Tags: Int J Syst Evol Microbiol Source Type: research

Rheinheimera sediminis sp. nov., a marine bacterium isolated from coastal sediment.
Abstract A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain YQF-1T, was isolated from coastal sediment in Jiangsu Province (PR China) and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 28 °C (range 4-40 °C) and pH 7 (range pH 6-11). Phylogenetic analysis based on 16S rRNA gene sequence indicated that YQF-1T was related to members of the genus Rheinheimera and shared the highest sequence identities with Rheinheimera mesophila DSM 29723T (98.5 %), followed by Rheinheimera tangshanensis DSM 19460T (98.4 %), Rheinheimera tilapiae R...
Source: International Journal of Systematic and Evolutionary Microbiology - December 4, 2019 Category: Microbiology Authors: Yu Q, Qi Y, Zhang H, Pu J Tags: Int J Syst Evol Microbiol Source Type: research

Flexivirga caeni sp. nov., isolated from activated sludge.
In this study, we describe the taxonomic characterization and classification of this bacterium by using the polyphasic approach. Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar. The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. This isoprenoid quinones included MK-8 (H4) and MK-8 (H6). The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan typ...
Source: International Journal of Systematic and Evolutionary Microbiology - December 4, 2019 Category: Microbiology Authors: Keum DH, Lee YJ, Lee SY, Im WT Tags: Int J Syst Evol Microbiol Source Type: research

Thalassocella blandensis gen. nov., sp. nov., a novel member of the family Cellvibrionaceae.
ió C, Pujalte MJ Abstract Strain ISS155T, isolated from surface Mediterranean seawater, has cells that are Gram-reaction-negative, motile, strictly aerobic chemoorganotrophic, oxidase-positive, unable to reduce nitrate to nitrite, and able to grow with cellulose as the sole carbon and energy source. It is mesophilic, neutrophilic, slightly halophilic and has a requirement for sodium and magnesium ions. Its 16S rRNA gene sequence places the strain among members of Cellvibrionaceae, in the Gammaproteobacteria, with Agarilytica rhodophyticola 017T as closest relative (94.3 % similarity). Its major cellular f...
Source: International Journal of Systematic and Evolutionary Microbiology - December 3, 2019 Category: Microbiology Authors: Lucena T, Arahal DR, Sanz-Sáez I, Acinas SG, Sánchez O, Aznar R, Pedrós-Alió C, Pujalte MJ Tags: Int J Syst Evol Microbiol Source Type: research

Borrelia maritima sp. nov., a novel species of the Borrelia burgdorferi sensu lato complex, occupying a basal position to North American species.
Abstract Borrelia species are vector-borne parasitic bacteria with unusual, highly fragmented genomes that include a linear chromosome and linear as well as circular plasmids that differ numerically between and within various species. Strain CA690T, which was cultivated from a questing Ixodes spinipalpis nymph in the San Francisco Bay area, CA, was determined to be genetically distinct from all other described species belonging to the Borrelia burgdorferi sensu lato complex. The genome, including plasmids, was assembled using a hybrid assembly of short Illumina reads and long reads obtained via Oxford Nanopore Tec...
Source: International Journal of Systematic and Evolutionary Microbiology - December 3, 2019 Category: Microbiology Authors: Margos G, Fedorova N, Becker NS, Kleinjan JE, Marosevic D, Krebs S, Hui L, Fingerle V, Lane RS Tags: Int J Syst Evol Microbiol Source Type: research

Anaerosphaera multitolerans sp. nov., a salt-tolerant member of the family Peptoniphilaceae isolated from a mesophilically operated biogas fermenter fed with maize silage.
Abstract In this work, we succeeded in the isolation of a novel species out of a mesophilically operated biogas fermenter fed with maize silage. Strains GS7-6-2T, GS-7K2 and GS-0K3 were isolated from three individual enrichment cultures. 16S rRNA gene sequence comparisons indicated that the isolates had 100 % sequence identity and were most closely related to Anaerosphaera amininiphila WN036T, with which they shared a 16S rRNA gene sequence similarity of 93.1 %. As a representative, strain GS7-6-2T was further characterized. Strain GS7-6-2T was mesophilic with its growth optimum at 30 °C and a pH ra...
Source: International Journal of Systematic and Evolutionary Microbiology - December 3, 2019 Category: Microbiology Authors: Rettenmaier R, Liebl W, Zverlov VV Tags: Int J Syst Evol Microbiol Source Type: research

Reclassification of the Clostridium clostridioforme and Clostridium sphenoides clades as Enterocloster gen. nov. and Lacrimispora gen. nov., including reclassification of 15 taxa.
Abstract The genus Clostridium belongs to the family Clostridiaceae. However, many species with the genus name Clostridium are found in different families and even crossing into a different phylum. Motivated by recently completed genome sequences, we propose the reclassification of two separate clades that include misclassified Clostridium species which phylogenetically lie within the family Lachnospiraceae, known for being benign members of gut microbiomes and for their plant-degrading capabilities. We use several phylogenetic and phylogenomic perspectives as well as phenotypic comparisons to gain insight into th...
Source: International Journal of Systematic and Evolutionary Microbiology - November 29, 2019 Category: Microbiology Authors: Haas KN, Blanchard JL Tags: Int J Syst Evol Microbiol Source Type: research

Asticcacaulis tiandongensis sp. nov., a new member of the genus Asticcacaulis, isolated from a cave soil sample.
Abstract A Gram-stain negative, aerobic, motile and rod-shaped bacterium, designated strain 3.1105T, was isolated from a karst district soil sample collected from Tiandong cave, Guizhou province, south-west PR China. The isolate grew at 10-40 °C and pH 5.0-8.0 and tolerated up to 1 % NaCl (w/v) on R2A medium, with optimal growth at 25-30 °C, pH 7.0 and 0 % NaCl (w/v). Cells showed oxidase-positive and catalase-positive reactions. The respiratory quinone was Q-10. The predominant cellular fatty acids contained C18 : 1ω7c 11-methyl, summed feature 8 (C18 : 1ω7c or...
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Zhou XK, Huang Y, Li M, Zhang XF, Wei YQ, Qin SC, Zhang TK, Wang XJ, Liu JJ, Wang L, Liu ZY, Mo MH Tags: Int J Syst Evol Microbiol Source Type: research

Torulaspora nypae sp. nov., a novel yeast species isolated from nipa (Nypa fruticans Wurmb.) inflorescence sap in southern Thailand.
Abstract Two strains (YSP-384 and YSP-399), representing a novel Torulaspora species, were isolated from two nipa inflorescence sap samples collected in Trang province in the southern part of Thailand. The two strains had identical sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions. The two strains were closest to Torulaspora maleeae CBS 10694T, but with 1.1 % nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 5.2 % nucleotide substitutions in the ITS regions. Phylogenetic analysis based on the concatenated sequences of the ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Kaewwichian R, Khunnamwong P, Am-In S, Jindamorakot S, Limtong S Tags: Int J Syst Evol Microbiol Source Type: research

Sphingomonas populi sp. nov., isolated from bark of Populus × euramericana.
Sphingomonas populi sp. nov., isolated from bark of Populus × euramericana. Int J Syst Evol Microbiol. 2019 Nov 28;: Authors: Li Y, Bian DR, Chang JP, Guo LM, Yang XQ Abstract One Gram-stain-negative, aerobic, motile bacterial strain, 3-7T, was isolated from symptomatic canker bark tissue of Populus × euramericana. 16S rRNA gene sequence data revealed that the novel isolate shared highest similarity with Sphingomonas panacis DCY99T (98.8 %), and
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Li Y, Bian DR, Chang JP, Guo LM, Yang XQ Tags: Int J Syst Evol Microbiol Source Type: research

Shewanella maritima sp. nov., a facultative anaerobic marine bacterium isolated from seawater, and emended description of Shewanella intestini.
Abstract A Gram-stain-negative, motile, facultative anaerobic rod-shaped marine bacterium, designated strain D4-2T, was isolated from a sample of seawater collected at Dong-do marina, Dokdo Island, in the East Sea of the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain D4-2T was affiliated with members of genus Shewanella and closely related to Shewanella intestini XMDDZSB0408T (97.4%), followed by Shewanella gelidii RZB5-4T (96.7 %) and Shewanella inventionis KX27T (96.1 %). D4-2T has a single circular chromosome of 4.72 Mbp with a DNA G+C content of 44.5 mol%...
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Bae SS, Jung YH, Baek K Tags: Int J Syst Evol Microbiol Source Type: research

Pseudooceanicola onchidii sp. nov., isolated from a marine invertebrate from the South China Sea.
Abstract A novel bacterial strain, XY-99T, was isolated from the epidermis of a marine invertebrate of the genus Onchidium from seawater of the South China Sea. The cells of the strain were aerobic, Gram-stain-negative, non-motile, and oval-shaped (0.8-1.0 µm wide and 1.0-1.5 µm long) without a flagellum. The strain grew at temperatures of 15-37 °C (optimum, 35-37 °C), at pH 5.5-9.5 (optimum 7.5), and at NaCl concentrations of 0-12.0 % (w/v) (optimum 1.5-3.0 %). The major fatty acids (>10 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18...
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Yin Q, Song ZM, Liang J, Wang Y, Zheng X, Li S, Xu Y Tags: Int J Syst Evol Microbiol Source Type: research

Marinobacter fonticola sp. nov., isolated from deep sea cold seep sediment.
In this study, we report a novel Gram-negative bacterium, designated as strain CS412T, isolated from deep-sea sediment collected in a cold seep area of the South China Sea. Growth of strain CS412T occurred at 4-40 °C (optimum, 28 °C), pH 5.0-11.0 (optimum, pH 6.0) and with 0-19 % (w/v) NaCl (optimum, 1-2 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain CS412T belonged to the genus Marinobacter. The closest phylogenetic neighbours of strain CS412T were Marinobacter pelagius HS225T (96.9 %), Marinobacter szutsaonensis NTU-104T (96.8%), Marinobacter santoriniensi...
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Sun QL, Sun YL, Sun YY, Luan ZD, Lian C Tags: Int J Syst Evol Microbiol Source Type: research

Thermaurantimonas aggregans gen. nov., sp. nov., a moderately thermophilic heterotrophic aggregating bacterium isolated from microbial mats at a terrestrial hot spring.
Abstract A moderately thermophilic and heterotrophic bacterial strain, LAT, was isolated from microbial mats sampled at a hot spring in Nakabusa, Nagano, Japan. The cells of strain LAT were aerobic, Gram-stain-negative, non-sporulating, non-motile and rod-shaped (2.0-4.1 µm long). The formation of dense cell aggregates in liquid medium was a unique characteristic of the strain. Strain LAT grew optimally at 50°C and at pH 7.5. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbour of strain LAT was Schleiferia thermophila JCM 30197T with 94.1 % sequence similarity....
Source: International Journal of Systematic and Evolutionary Microbiology - November 28, 2019 Category: Microbiology Authors: Iino T, Kawai S, Yuki M, Dekio I, Ohkuma M, Haruta S Tags: Int J Syst Evol Microbiol Source Type: research

Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14.
Abstract A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8-2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42-60 °C and pH 6.5-8.5, the optima being 50-54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemo...
Source: International Journal of Systematic and Evolutionary Microbiology - November 26, 2019 Category: Microbiology Authors: Kochetkova TV, Zayulina KS, Zhigarkov VS, Minaev NV, Chichkov BN, Novikov AA, Toshchakov SV, Elcheninov AG, Kublanov IV Tags: Int J Syst Evol Microbiol Source Type: research

Desertihabitans brevis sp. nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
Abstract A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China. The strain was examined by a polyphasic approach to clarify its taxonomic position. Cells of the isolate were Gram-staining-positive, aerobic, non-motile and short-rod shaped. Strain 16Sb5-5T grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl. The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP). Ribose, arabinose and glucose were detected i...
Source: International Journal of Systematic and Evolutionary Microbiology - November 26, 2019 Category: Microbiology Authors: Liu SW, Li FN, Liu HY, Yu LY, Sun CH Tags: Int J Syst Evol Microbiol Source Type: research

Pseudorivibacter rhizosphaerae gen. nov., sp. nov., isolated from rhizosphere soil of Camellia sinensis (L.) O. Ktze and emended description of the genus Rivibacter.
Abstract A Gram-stain-negative, facultative anaerobic, motile and straight rod-shaped bacterium, designated strain C1-9T, was isolated from rhizosphere soil of Camellia sinensis (L.) O. Ktze collected from a tea garden in Huize, south-western PR China. Cells were oxidase-positive and catalase-negative. Growth occurred at 20-40 °C and pH 6.0-10.0, with an optimal growth at 30 °C and pH 7.0. The respiratory quinone was detected as ubiquinone-8 (Q-8). The major fatty acids were identified as summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and summed featur...
Source: International Journal of Systematic and Evolutionary Microbiology - November 22, 2019 Category: Microbiology Authors: Zhou XK, Li QQ, Dong LM, Yang DQ, Liu L, Fang BZ, Jeon CO, Li WJ, Duan YQ Tags: Int J Syst Evol Microbiol Source Type: research

Nitrincola tapanii sp. nov., a novel alkaliphilic bacterium from An Indian Soda Lake.
Abstract A novel Gram-stain-negative bacterial strain designated as MEB193T was isolated from a sediment sample collected from Lonar Lake, India. The cells were motile, non-spore-forming and rod-shaped. The strain was oxidase- and catalase-positive. It grew optimally at pH 9.0 and at 1 % (w/v) NaCl concentration at 30 °C. Based on 16S rRNA gene sequence similarity, MEB193T belongs to genus Nitrincola, with Nitrincola alkalilacustris ZV-19T (95.89 %) and Nitrincola lacisaponensis 4CAT (95.87 %) as its closest neighbours. The major fatty acid was summed feature 8 comprising C18:1ω7c/C18:1&omeg...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Joshi A, Thite S, Dhotre D, Moorthy M, Joseph N, Ramana VV, Shouche Y Tags: Int J Syst Evol Microbiol Source Type: research

Dyadobacter flavalbus sp. nov., isolated from lake sediment.
Abstract A novel bacterial strain, designated NS28T, was isolated from interfacial sediment sampled at Taihu Lake, PR China. Cells were rod-shaped, Gram-negative, aerobic and non-motile on Reasoner's 2A medium. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NS28T was most closely related to species from the genus Dyadobacter, with 98.4 and 96.0 % 16S rRNA gene sequence similarity to its closest phylogenetic neighbours Dyadobacter sediminis CGMCC 1.12895T and Dyadobacter luticola CCTCC AB 2017091T, respectively. MK-7 was the only cellular menaquinone. The major fatty aci...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Qu JH, Yue YF, Zhou J, Qu LB, Wang LF Tags: Int J Syst Evol Microbiol Source Type: research

Pseudomonas rhizoryzae sp. nov., isolated from rice.
Abstract Two yellow-pigmented, Gram-stain-negative and rod-shaped bacterial strains, designated as RY24T and ZYY160, were isolated from rice. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strains RY24T and ZYY160 belonged to the genus Pseudomonas, and the 16S rRNA gene sequence similarity was 100 % The DNA homology between the two strains was 99.7 %. The 16S rRNA and rpoD gene sequences of the two strains showed highest similarity values to Pseudomonas oryzihabitans CGMCC 1.3392T and Pseudomonas psychrotolerans DSM 15758T (sharing 99.31 and 94.34 %, respectively). The major fatty ac...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Wang X, He SW, Guo HB, Thin KK, Gao JS, Wang Y, Zhang XX Tags: Int J Syst Evol Microbiol Source Type: research

Tamlana fucoidanivorans sp. nov., isolated from algae collected in China.
Abstract A Gram-stain-negative, aerobic, non-motile and rod-shaped marine bacterium, CW2-9T, was isolated from algae collected from Fujian Province in PR China. 16S rRNA gene sequence analysis showed that this strain was affiliated with the genus Tamlana in the family Flavobacteriaceae of the class Flavobacteriia and was very similar to the type strain Tamlana sedimentorum MCCC 1A10799T (96.3 % sequence similarity). The whole genome of strain CW2-9T comprised 3 997 513 bp with a G+C content of 34.3 mol%. The average nucleotide identity value between strain CW2-9T and T. sedimentorum MCCC 1A10799T w...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Li J, Xu Y, Feng J, Zhong M, Xie Q, Peng T, Hu Z Tags: Int J Syst Evol Microbiol Source Type: research

Gudongella oleilytica gen. nov., sp. nov., an aerotorelant bacterium isolated from Shengli oilfield and validation of family Tissierellaceae.
Abstract A novel Gram-stain-positive, rod shaped and anaerobic bacterium, designated as W6T, was isolated from Shengli oilfield in China. Strain W6T was observed to grow from 20 to 45 °C with pH 6.5-9.0 (optimally at 40 °C and pH of 7.5) and without addition of NaCl. The major cellular fatty acids were iso-C15 : 0 (29.1%), C14 : 0 (27.0%) and C16 : 0 (12.2%), and the main polar lipids were lipids (L) and aminolipids (AL). The DNA G+C content is 42.9 mol%. Based on 16S rRNA gene sequence analysis, strain W6T showed highest similarities to Tissierella creatinini DSM 9508T (94...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Wu K, Dai L, Tu B, Zhang X, Zhang H, Deng Y, Lawson PA, Cheng L Tags: Int J Syst Evol Microbiol Source Type: research

Sediminibacillus terrae sp. nov., a moderate halophile isolated from non-saline farm soil.
Abstract A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China. Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C). Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Wu QL, Chen JH, Deng LY, Liu ZX, He JW, Liu ZX, Yang LL, Tang SK, Chen YG Tags: Int J Syst Evol Microbiol Source Type: research

Haliea alexandrii sp. nov., isolated from phycosphere microbiota of the toxin-producing dinoflagellate Alexandrium catenella.
Abstract A Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, named strain LZ-16-2T, was isolated from the phycosphere microbiota of the paralytic shellfish poisoning toxin-producing marine dinoflagellate Alexandrium catenella LZT09. Strain LZ-16-2T grew optimally at 28 °C at pH 6.5 and with 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain LZ-16-2T fell within the genus Haliea and was most closely related to Haliea salexigens DSM 19537T, with which the new isolate exhibited 98.5 % 16S rRNA gene sequence similarity. The major resp...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Yang Q, Jiang Z, Zhou X, Zhang R, Xie Z, Zhang S, Wu Y, Ge Y, Zhang X Tags: Int J Syst Evol Microbiol Source Type: research

Genome analysis-based reclassification of Enterobacter tabaci Duan et al. 2016 as a later heterotypic synonym of Enterobacter mori Zhu et al. 2011.
Abstract We performed a comparative genomic analysis to clarify the taxonomic relationship between the two Enterobacter species, Enterobacter tabaci and Enterobacter mori. Whole genome sequences of types strains of the two species became available recently. Average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) between the two type strains were determined. Type strains of the two species had a 97.25 % ANI and an 80.4 % isDDH value, which are above the well-recognized cutoffs (≥95-96 % ANI and ≥70 % isDDH) for bacterial species delineation. The two strains have similar ov...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Wu W, Zong Z Tags: Int J Syst Evol Microbiol Source Type: research

Mycosarcoma aegyptiacum sp. nov., an antagonistic polymorphic basidiomycetous yeast related to smut fungi.
Abstract Six polymorphic yeast strains with strong antifungal activities isolated from dicot plants in an alkaline-lake desert region were subjected to taxonomic examination. The phylogenetic trees reconstructed by using neighbour-joining, maximum-likelihood and Bayesian methods from concatenated D1/D2 and ITS-5.8S-ITS2 sequences revealed phylogenetic affinity to Ustilaginaceae, but the large phylogenetic distance separating the isolates from the most closely related groups of species indicates that they represent a separate species. The sequences of the genes coding for the LSU rDNA, act1, rpb2 and a protein of u...
Source: International Journal of Systematic and Evolutionary Microbiology - November 21, 2019 Category: Microbiology Authors: Sipiczki M Tags: Int J Syst Evol Microbiol Source Type: research

Saccharomycopsis oxydans sp. nov., a new non-fermentative member in the genus Saccharomycopsis isolated from a traditional dairy product of Iran.
Abstract A total of 21 yeast isolates were recovered as part of a research project on biodiversity of yeasts in traditional dairy products in Alborz province, Iran. Standard protocols were used to carry out phenotypic, biochemical, physiological characterization and the phylogenetic analysis of combined the D1/D2 domain of the large ribosomal subunit (26S or LSU) and ITS region sequences. Five strains represented a potential new ascomycetous yeast species. Ascospore formation was not observed in these strains, and they did not ferment the examined carbon sources. Phylogenetic analysis placed these isolates in a we...
Source: International Journal of Systematic and Evolutionary Microbiology - November 20, 2019 Category: Microbiology Authors: Hajihosseinali M, Nasr S, Amoozegar MA, Yurkov A Tags: Int J Syst Evol Microbiol Source Type: research

Description of Paenibacillus antri sp. nov. and Paenibacillus mesophilus sp. nov., isolated from cave soil.
Abstract Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China. The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively. Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was...
Source: International Journal of Systematic and Evolutionary Microbiology - November 20, 2019 Category: Microbiology Authors: Narsing Rao MP, Dong ZY, Kan Y, Zhang K, Fang BZ, Xiao M, Kang YQ, Li WJ Tags: Int J Syst Evol Microbiol Source Type: research

Tsukamurella asaccharolytica sp. nov., Tsukamurella conjunctivitidis sp. nov. and Tsukamurella sputi sp. nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
Abstract Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species. 16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 18, 2019 Category: Microbiology Authors: Teng JLL, Fong JYH, Fok KMN, Lee HH, Chiu TH, Tang Y, Ngan AHY, Wong SSY, Que TL, Lau SKP, Woo PCY Tags: Int J Syst Evol Microbiol Source Type: research

Naasia lichenicola sp. nov., an actinobacterium isolated from lichen.
Abstract A Gram-stain-positive, yellow-pigmented, catalase-positive and oxidase-negative, strictly aerobic actinobacterium, designated strain YIM 131853T, was isolated from lichen collected from the South Bank of the Baltic Sea. The novel strain was non-spore-forming, short rod-shaped and motile with a single polar flagellum. The strain could grow at 4-37 °C (optimum, 28 °C), at pH 4.0-12.0 (pH 6.0) and at 0-3 % (w/v) NaCl (1 %). The DNA G+C content of strain YIM 131853T based on the draft genome sequence was 68.3 mol%. Predominant cellular fatty acids (>10 %) were identified ...
Source: International Journal of Systematic and Evolutionary Microbiology - November 18, 2019 Category: Microbiology Authors: Jiang LQ, Zhang K, Li GD, Wang XY, Shi SB, Li QY, An DF, Lang L, Wang LS, Jiang CL, Jiang Y Tags: Int J Syst Evol Microbiol Source Type: research

Arenibacter aquaticus sp. nov., a marine bacterium isolated from surface sea water in the South China Sea.
Abstract A Gram-stain-negative and facultatively anaerobic bacterial strain, designated GUOT, was isolated from surface water collected from the South China Sea. Cells were non-flagellate, yellow, non-spore-forming and rod-shaped. The 16S rRNA gene sequence comparisons with species in the genus Arenibacter showed that strain GUOT shares the highest similarity of 97.5 % with Arenibacter echinorum and Arenibacter palladensis. Average nucleotide identity and digital DNA-DNA hybridization values between strain GUOT and its related type strains were 77.1-78.4% and 20.8-26.2 % respectively. Growth of s...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Guo J, Sun J, Xu Y, Fang L, Jiao N, Zhang R Tags: Int J Syst Evol Microbiol Source Type: research

Ktedonosporobacter rubrisoli gen. nov., sp. nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam. nov.
Abstract A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China. The strain grew at 25-45 °C and at pH 4.0-7.0, and was able to tolerate up to 50 mM Zn2+. The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Yan B, Guo X, Liu M, Huang Y Tags: Int J Syst Evol Microbiol Source Type: research

Flavobacterium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa.
Abstract A Gram-stain-negative, yellow-pigmented, strictly aerobic bacterium, designated strain MaA-Y11T, was isolated from a culture of Microcystis aeruginosa in the Republic of Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain MaA-Y11T was observed at 25-37 °C (optimum, 30 °C) and pH 6.0-9.0 (optimum, 7.0) and in the presence of 0-1.0 % (w/v) NaCl (optimum, 0 %). Strain MaA-Y11T did not produce flexirubin-type pigments. Strain MaA-Y11T contained iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Seo YL, Jeong SE, Jin HM, Jeon CO Tags: Int J Syst Evol Microbiol Source Type: research

Formosa maritima sp. nov., isolated from coastal sediment.
Abstract A Gram-stain-negative, strictly aerobic, gliding-motile, rod-shaped and orange-pigmented bacterium, designated 1494T, was isolated from marine sediment collected off the coast of Weihai, PR China. Strain 1494T was found to grow at 4-37 °C (optimum, 30 °C), at pH 6.0-9.0 (pH 7.0) and in the presence of 0-8 % (w/v) NaCl (2 %). Cells were positive for oxidase and catalase activity. The results of 16S rRNA gene based phylogenetic analysis revealed that strain 1494T belonged to the genus Formosa and exhibited the highest sequence similarity to Formosa spongicola KCTC 22662 T (98.4 %). Menaquinone...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Cao WR, Lu DC, Li YJ, Sun XK, Sun YY, Saren G, Du ZJ Tags: Int J Syst Evol Microbiol Source Type: research

Pseudomonas saxonica sp. nov., isolated from raw milk and skimmed milk concentrate.
Abstract A polyphasic approach was used to investigate the taxonomic status of two bacterial strains, WS 5072T and WS 5092, isolated from skimmed milk concentrate and raw cow's milk. The 16S rRNA and rpoD gene sequences affiliated the strains to the same, hitherto unknown, Pseudomonas species. Further examinations of the draft genomes based on multilocus sequence analysis and average nucleotide identity confirmed the presence of a novel Pseudomonas species. It was most closely related to Pseudomonas fragi DSM 3456T with 86.3 % ANIm. The DNA G+C content of strain WS 5072T was 56.3 mol%. Cells were aerobic,...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Hofmann K, Huptas C, Doll EV, Scherer S, Wenning M Tags: Int J Syst Evol Microbiol Source Type: research

Rubrivivax albus sp. nov., isolated from a freshwater pond.
Abstract Strain ICH-3T, isolated from a freshwater pond in Taiwan 9ROC), was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that ICH-3T is affiliated with the species in the genus Rubrivivax. ICH-3T was most closely related to Rubrivivax benzoatilyticus JA2T and Rubrivivax gelatinosus NCIB 8290T with 97.5 and 97.4 % 16S rRNA gene sequence similarity. The average nucleotide identity and digital DNA-DNA hybridization identity between ICH-3T and the two closely related strains were 7...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Sheu SY, Li ZH, Young CC, Chen WM Tags: Int J Syst Evol Microbiol Source Type: research

Microbispora catharanthi sp. nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
Abstract Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches. The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia. It contained meso-diaminopimelic acid in cell wall peptidoglycans. The major menaquinones were MK-9 (H4) and MK-9 (H2). The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Klykleung N, Yuki M, Kudo T, Ohkuma M, Phongsopitanun W, Pittayakhajonwut P, Tanasupawat S Tags: Int J Syst Evol Microbiol Source Type: research

Serinibacter arcticus sp. nov., isolated from a thawing ancient ice wedge.
Abstract A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia). Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming. Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С. The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine. The polar lipids were phosphatidylglycerol, lysophosp...
Source: International Journal of Systematic and Evolutionary Microbiology - November 15, 2019 Category: Microbiology Authors: Filippova SN, Surgucheva NA, Detkova EN, Rakitin AL, Beletsky AV, Grouzdev DS, Kolganova TV, Mulyukin AL Tags: Int J Syst Evol Microbiol Source Type: research

Cryptosporangium phraense sp. nov., isolated from plant litter.
Abstract A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand. Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium. The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium. The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium. The values of DNA-DNA relatedne...
Source: International Journal of Systematic and Evolutionary Microbiology - November 14, 2019 Category: Microbiology Authors: Suriyachadkun C, Ngaemthao W, Pujchakarn T, Chunhametha S Tags: Int J Syst Evol Microbiol Source Type: research

Saturnispora mangrovi f.a., sp. nov. from Syhat mangrove, Saudi Arabia.
Abstract Strain SY-07 was isolated from decaying leaves of Avicennia marina collected from Syhat mangroves, Dammam city, Arabian Gulf, Saudi Arabia. Phylogenetic analyses of three genes [D1/D2 region of the LSU and SSU rRNA genes and internal transcribed spacer (ITS) region] showed that strain SY-07 represents a novel species of the genus Saturnispora distinct from closely related species. Saturnispora mendoncae was the most closely related species with an LSU gene sequence similarity of 89.3 % (58 nucleotide substitutions and four indels out of 578 nt), 97 % similarity for the SSU gene (42 nucleotide substitu...
Source: International Journal of Systematic and Evolutionary Microbiology - November 14, 2019 Category: Microbiology Authors: El-Samawaty AEMA, Boekhout T, Yassin MA, Abdel-Wahab MA Tags: Int J Syst Evol Microbiol Source Type: research

Seonamhaeicola maritimus sp. nov., isolated from coastal sediment.
Abstract A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and orange-pigmented bacterium, designated 1505T, was isolated from marine sediment that was obtained off the coast of Weihai, PR China. Strain 1505T was found to grow at 10-35 °C (optimum, 28 °C), at pH 6.0-9.0 (optimum, 7.5) and in the presence of 1-4 % (w/v) NaCl (optimum, 2 %). Cells were positive for oxidase and catalase activity. The 16S rRNA gene based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain 1505T were Seonamhaeicola algicola Gy8T (97.1 %), Seonamhaeicola marinus B011T (96....
Source: International Journal of Systematic and Evolutionary Microbiology - November 12, 2019 Category: Microbiology Authors: Cao WR, Lu DC, Sun XK, Sun YY, Saren G, Yu XK, Du ZJ Tags: Int J Syst Evol Microbiol Source Type: research

Nesterenkonia salmonea sp. nov. and Nesterenkonia sphaerica sp. nov., isolated from the Southern Atlantic Ocean.
Abstract Two Gram-positive, aerobic, non-motile and non-spore-forming actinobacteria, strains GY074T and GY239T, were isolated from deep-sea sediment of the Southern Atlantic Ocean. The results of phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus Nesterenkonia, and showed a sequence similarity of 98.3 % between the two strains and similarites of 94.3-97.2 % with respect to Nesterenkonia species with validly published names. Based on whole-genome sequences, the values of in silico DNA-DNA hybridization and the average nucleotide identity between strains GY074T and GY239T wer...
Source: International Journal of Systematic and Evolutionary Microbiology - November 12, 2019 Category: Microbiology Authors: Zhang G, Wang L, Xie F, Pei S, Jiang L Tags: Int J Syst Evol Microbiol Source Type: research