Molecules, Vol. 29, Pages 1864: The Antioxidant, Anti-Inflammatory and Moisturizing Effects of Camellia oleifera Oil and Its Potential Applications
This study determined the physicochemical properties of CO oil extracted via direct pressing, identified its main components using GC-MS, and evaluated its antioxidant, moisturizing, and anti-inflammatory activities. The results revealed that CO oil’s acid, peroxide, iodine, and saponification values were 1.06 ± 0.031 mg/g, 0.24 ± 0.01 g/100 g, 65.14 ± 8.22 g/100 g, and 180.41 ± 5.60 mg/g, respectively. CO oil’s tocopherol, polyphenol, and squalene contents were 82.21 ± 9.07 mg/kg, 181.37 ± 3.76 mg/kg, and ...
Source: Molecules - April 19, 2024 Category: Chemistry Authors: Lijun Zhou Yunlan Peng Zhou Xu Jingyi Chen Ningbo Zhang Tao Liang Tao Chen Yao Xiao Shiling Feng Chunbang Ding Tags: Article Source Type: research
Investigating the spatiotemporal dynamics of apple tree phyllosphere bacterial and fungal communities across cultivars in orchards
In this study, we investigated the determinants of the spatiotemporal variation in bacterial and fungal communities within the apple tree phyllosphere, employing 16S and ITS amplicon sequencing. Our research assessed the impact of key factors —plant compartment, site, time, and cultivar—on the composition and diversity of leaf and flower microbial communities. Our analyses, based on samples collected from three cultivars in three orchards in 2022, revealed that site and time are the strongest drivers of apple tree phyllosphere microb ial communities. Conversely, plant compartment and cultivar exhibited minor roles in e...
Source: Canadian Journal of Microbiology - April 18, 2024 Category: Microbiology Authors: Sophie Boutin Ema Lussier Isabelle Laforest-Lapointe Source Type: research
Heat shock protein 71 restricts mutation of porcine reproductive and respiratory syndrome virus nsp2 in vitro
In this study, the binding of PRRSV nsp2 to HSP71 protein was detected by using the IP/MS technique. And the inhibitory effect of HSP71 on nsp2 antagonistic activity was validated by measuring NF-kB luciferase reporter. According to stress from inhibitory effects, the amino acid variation profile of PRRSV nsp2 under HSP71 stress was further analyzed using second-generation sequencing. Surprisingly, the results indicated that HSP71 pressure limits the random mutations of PRRSV nsp2 and maintains the dominant PRRSV strain within the population. Mutant strain showed weaker antagonistic activity and replication capability in c...
Source: Comparative immunology, microbiology and infectious diseases. - April 18, 2024 Category: Allergy & Immunology Authors: Fengyu Xie Lei Kang Mengli Chen Tong Zhang Zongjie Li Donghua Shao Beibei Li Jianchao Wei Yafeng Qiu Manxiang Li Zhiyong Ma Ke Liu Source Type: research
Multi-locus sequence analysis of Anaplasma bovis in goats and ticks from Thailand, with the initial identification of an uncultured Anaplasma species closely related to Anaplasma phagocytophilum-like 1
This study aimed to undertake a genetic analysis of A. bovis and determine its prevalence in goats and ticks utilizing three genetic markers (16S rRNA, gltA, groEL). A total of 601 goat blood and 118 tick samples were collected from 12 sampling sites throughout Thailand. Two tick species, Haemaphysalis bispinosa (n = 109), and Rhipicephalus microplus (n = 9) were identified. The results herein showed that 13.8 % (83/601) of goats at several farms and 5 % (1/20) of ticks were infected with A. bovis. Among infected ticks, A. bovis and an uncultured Anaplasma sp. which are closely related to A. phagocytophilum-like 1, were de...
Source: Comparative immunology, microbiology and infectious diseases. - April 18, 2024 Category: Allergy & Immunology Authors: Aung Aung Duriyang Narapakdeesakul Apinya Arnuphapprasert Yudhi Ratna Nugraheni Chaiyawan Wattanachant Winai Kaewlamun Morakot Kaewthamasorn Source Type: research
Cereus jamacaru DC. (mandacaru) fruit as a source of lactic acid bacteria with in vitro probiotic-related characteristics and its protective effects on Pediococcus pentosaceus during lyophilization and refrigeration storage
This study isolated and identified autochthonous lactic acid bacteria (LAB) from mandacaru fruit and evaluated their potential probiotic and technological aptitudes in vitro, as well as the protective effects of freeze-dried mandacaru fruit on the most promising LAB isolate during lyophilization and refrigeration storage. Initially, 212 colonies were isolated from mandacaru fruit, and 34 were preliminarily identified as LAB. Thirteen isolates identified by 16S-rRNA sequencing as Pediococcus pentosaceus were negative for DNase, gelatinase, hemolytic, and biogenic amine production. The selected isolates showed proteolytic ac...
Source: International Journal of Food Microbiology - April 18, 2024 Category: Food Science Authors: Gracy Kelly Vieira de Vasconcelos Medeiros Ana Cristina Silveira Martins Mateus Gomes Vasconcelos Estef ânia Fernandes Garcia No ádia Priscila Araújo Rodrigues Thatyane Mariano Rodrigues de Albuquerque Vanessa Bordin Viera Maria L úcia da Conceição Source Type: research
Serratia montpellierensis sp. nov., Isolated from Laboratory-Reared Parasitic Wasps Psyttalia lounsburyii Silvestri and Psyttalia ponerophaga Silvestri (Hymenoptera: Braconidae)
Curr Microbiol. 2024 Apr 18;81(6):146. doi: 10.1007/s00284-024-03666-0.ABSTRACTTwo strains of bacteria, PsyLou2AT and PsyPon4B, were isolated from adult braconid wasps Psyttalia lounsburyii and Psyttalia ponerophaga, respectively. These laboratory-reared wasps were investigated as agents for biological control of the olive fruit fly, Bactrocera oleae. Analysis of 16S rRNA genes of the two isolates demonstrated that they were highly related and belonged to the genus Serratia. Genomic sequencing of these isolates revealed genomes of 5,152,551 bp and 5,154,385 bp for PsyLou2AT and PsyPon4B, respectively, and both genomes had ...
Source: Current Microbiology - April 18, 2024 Category: Microbiology Authors: Michael B Blackburn M élanie Tannières Michael E Sparks Dawn E Gundersen-Rindal Marie-Claude Bon Source Type: research
Regulation of lactose, glucose and sucrose metabolisms in S. thermophilus
Food Microbiol. 2024 Aug;121:104487. doi: 10.1016/j.fm.2024.104487. Epub 2024 Feb 12.ABSTRACTStreptococcus thermophilus is a bacterium widely used in the production of yogurts and cheeses, where it efficiently ferments lactose, the saccharide naturally present in milk. It is also employed as a starter in dairy- or plant-based fermented foods that contain saccharides other than lactose (e.g., sucrose, glucose). However, little is known about how saccharide use is regulated, in particular when saccharides are mixed. Here, we determine the effect of the 5 sugars that S. thermophilus is able to use, at different concentration ...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: C Gasser J M Faurie F Rul Source Type: research
Persistence of hepatitis E virus (HEV) subtypes 3c and 3e: Long-term cold storage and heat treatments
Food Microbiol. 2024 Aug;121:104529. doi: 10.1016/j.fm.2024.104529. Epub 2024 Mar 30.ABSTRACTHepatitis E virus (HEV) is the causative agent of foodborne infections occurring in high income countries mainly by consumption of undercooked and raw pork products. The virus is zoonotic with pigs and wild boars as the main reservoirs. Several studies proved the presence of HEV-RNA in pork liver sausages, pâté and other pork by-products. However, the detection of HEV nucleic acids does not necessary correspond to infectious virus and information on the persistence of the virus in the food is still limited. To which extent and ho...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Marina Monini Giovanni Ianiro Luca De Sabato Marta Bivona Fabio Ostanello Ilaria Di Bartolo Source Type: research
Comprehensive identification of pathogenic microbes and antimicrobial resistance genes in food products using nanopore sequencing-based metagenomics
In conclusion, this comprehensive study offers invaluable data that can contribute to food safety assessments and serve as a basis for quality indicators.PMID:38637066 | DOI:10.1016/j.fm.2024.104493 (Source: Food Microbiology)
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Annie Wing-Tung Lee Iain Chi-Fung Ng Evelyn Yin-Kwan Wong Ivan Tak-Fai Wong Rebecca Po-Po Sze Kit-Yu Chan Tsz-Yan So Zhipeng Zhang Sharon Ka-Yee Fung Sally Choi-Ying Wong Wing-Yin Tam Hiu-Yin Lao Lam-Kwong Lee Jake Siu-Lun Leung Chloe Toi-Mei Chan Timothy Source Type: research
Effects of TrPLDs on the pathogenicity of Trichothecium roseum infected apple fruit
Food Microbiol. 2024 Aug;121:104496. doi: 10.1016/j.fm.2024.104496. Epub 2024 Feb 23.ABSTRACTPhospholipase D plays a critical regulatory role in the pathogenicity of filamentous fungi. However, the molecular mechanism of PLD regulating the pathogenicity of filamentous fungi has not been reported. In this research, the previously constructed TrPLD1 and TrPLD2 (TrPLDs) mutants were used as test strains. Firstly, the function of TrPLDs in Trichothecium roseum was studied. Then, the effects of TrPLDs on the pathogenicity of T. roseum and the quality of the inoculated apples were verified. The results suggested that the deletio...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Qili Liu Qianqian Zhang Huali Xue Yang Bi Xi Yang Yuanyuan Zong Zhiguang Liu Prusky Dov Source Type: research
Structure and biological activity in vitro of Flagellin and its mutants from Escherichia coli Nissle 1917
This study utilized AlphaFold2, SERS (Surface-enhanced Raman spectroscopy), and CD (circular dichroism) techniques to analyze the structural characteristics of FliCEcN and its mutants, FliCΔ174-506 and FliCΔ274-406, and tested their immune effects by stimulating Caco-2 cells in vitro. The results indicate that the D2 and D3 domains of FliCEcN have more complex interactions compared to the D1-D2 domain pair., and these domains also play a role in molecular docking with TLR5 (Toll-like receptor 5). Furthermore, FliCΔ274-406 has more missing side chain and characteristic amino acid peaks than FliCΔ174-506. The FliCEcN gro...
Source: Archives of Microbiology - April 18, 2024 Category: Microbiology Authors: Shuang Li Ming Wen Guilan Wen Ying Yang Source Type: research
Hi-C metagenomics facilitate comparative genome analysis of bacteria and yeast from spontaneous beer and cider
Food Microbiol. 2024 Aug;121:104520. doi: 10.1016/j.fm.2024.104520. Epub 2024 Mar 26.ABSTRACTSequence-based analysis of fermented foods and beverages' microbiomes offers insights into their impact on taste and consumer health. High-throughput metagenomics provide detailed taxonomic and functional community profiling, but bacterial and yeast genome reconstruction and mobile genetic elements tracking are to be improved. We established a pipeline for exploring fermented foods microbiomes using metagenomics coupled with chromosome conformation capture (Hi-C metagenomics). The approach was applied to analyze a collection of spo...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Ignat V Sonets Mikhail A Solovyev Valeriia A Ivanova Petr A Vasiluev Aleksey V Kachalkin Sofia D Ochkalova Anton I Korobeynikov Sergey V Razin Sergey V Ulianov Alexander V Tyakht Source Type: research
Microbial composition and viability of natural whey starters used in PDO Comt é cheese-making
Food Microbiol. 2024 Aug;121:104521. doi: 10.1016/j.fm.2024.104521. Epub 2024 Mar 19.ABSTRACTNatural whey starters (NWS) are cultures with undefined multiple-strains species commonly used to speed up the fermentation process of cheeses. The aim of this study was to explore the diversity and the viability of Comté cheese NWS microbiota. Culture-dependent methods, i.e. plate counting and genotypic characterization, and culture-independent methods, i.e. qPCR, viability-qPCR, fluorescence microscopy and DNA metabarcoding, were combined to analyze thirty-six NWS collected in six Comté cheese factories at two seasons. Our resu...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Jade Lutin Franck Dufrene Philippe Guyot Romain Palme Christine Achilleos Yvette Bouton Solange Buchin Source Type: research
Correlation between the bacterial community succession and purine compound changes during Huangjiu fermentation
This study is helpful to scientifically understand the formation mechanism of purines, providing a basis for screening functional strains of purine degrading to accurately regulate purine level in Huangjiu.PMID:38637084 | DOI:10.1016/j.fm.2024.104522 (Source: Food Microbiology)
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Xianglin Wang Guolin Cai Dianhui Wu Jian Lu Source Type: research
Fungicidal efficiency of DBD cold plasma against Aspergillus niger on dried jujube
This study investigated the fungicidal efficiency and mechanism of action of dielectric barrier discharge cold atmosphere plasma (DBD-CAP) in inactivating Aspergillus niger (A. niger) spores. The disinfection efficacy and quality of dried jujube used as the processing application object were also studied. The results indicated that the Weibull + Tail model performed better for spore inactivation curves at different voltages among various treatment times, and the spore cells were reduced by 4.05 log (cfu/mL) in spores suspension at 70 kV after 15 min of treatment. This disinfection impact was further supported by scanning e...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Qiaoyun Wei Yuan Yuan Jianhao Zhang Jin Wang Source Type: research
