Detection of risk areas in dairy powder processes: The development of thermophilic spore forming bacteria taking into account their growth limits
Int J Food Microbiol. 2024 Apr 22;418:110716. doi: 10.1016/j.ijfoodmicro.2024.110716. Online ahead of print.ABSTRACTAnoxybacillus flavithermus, Geobacillus stearothermophilus and Bacillus licheniformis are the main contaminants found in dairy powders. These spore-forming thermophilic bacteria, rarely detected in raw milk, persist, and grow during the milk powder manufacturing process. Moreover, in the form of spores, these species resist and concentrate in the powders during the processes. The aim of this study was to determine the stages of the dairy powder manufacturing processes that are favorable to the growth of such ...
Source: International Journal of Food Microbiology - April 26, 2024 Category: Food Science Authors: Delaunay Louis Postollec Florence Leguerinel Ivan Mathot Anne-Gabrielle Source Type: research

Pedobacter rhodius sp. nov. and Pedobacter punctiformis sp. nov., isolated from soil
Antonie Van Leeuwenhoek. 2024 Apr 26;117(1):72. doi: 10.1007/s10482-024-01963-z.ABSTRACTTwo Gram-staining negative, catalase- and oxidase-positive, pinkish-colored and rod-shaped strains, designated SJ11T and HCMS5-2 T, were isolated from soil in South Korea. The growth of strain SJ11T was observed from 15℃ to 35℃ (optimum, 30℃), from pH 6.0 to 11.0 (optimum, pH 6.0-7.0) and with NaCl 0-1% (w/v) (optimum, 0%) and that of strain HCMS5-2 T was observed from 4℃ to 40℃ (optimum, 25℃), from pH 6.0 to pH 8.0 (optimum, pH 7.0) and with NaCl 0-5% (w/v) (optimum, 0-1%). Phylogenetic analysis based on 16S rRNA gene seque...
Source: Antonie van Leeuwenhoek - April 26, 2024 Category: Microbiology Authors: Haejin Woo Geeta Chhetri Inhyup Kim Yoonseop So Sunho Park Yonghee Jung Taegun Seo Source Type: research

The role of insect gut microbiota in host fitness, detoxification and nutrient supplementation
Antonie Van Leeuwenhoek. 2024 Apr 26;117(1):71. doi: 10.1007/s10482-024-01970-0.ABSTRACTInsects are incredibly diverse, ubiquitous and have successfully flourished out of the dynamic and often unpredictable nature of evolutionary processes. The resident microbiome has accompanied the physical and biological adaptations that enable their continued survival and proliferation in a wide array of environments. The host insect and microbiome's bidirectional relationship exhibits their capability to influence each other's physiology, behavior and characteristics. Insects are reported to rely directly on the microbial community to...
Source: Antonie van Leeuwenhoek - April 26, 2024 Category: Microbiology Authors: U Shamjana Deepa Azhchath Vasu Preety Sweta Hembrom Karunakar Nayak Tony Grace Source Type: research

Rhein kills < em > Actinobacillus pleuropneumoniae < /em > , reduces biofilm formation, and effectively treats bacterial lung infections in mice
Conclusions. This study reports the activity of Rhein against A. pleuropneumoniae and its mechanism, and reveals the ability of Rhein to treat A. pleuropneumoniae infection in mice, laying the foundation for the development of new drugs for bacterial infections.PMID:38668646 | DOI:10.1099/jmm.0.001826 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - April 26, 2024 Category: Microbiology Authors: Haifeng Ding Yilin Bai Weiyu Luo Hao Li Chunling Zhu Xueqin Zhao Huarun Sun Yuliang Wen Wei Zhang Shouping Zhang Bo Wen Ruibiao Wang Longfei Zhang Xuehan Liu Jiyuan Shen Jianhe Hu Lei Wang Yueyu Bai Chengshui Liao Yundi Wu Xilong Wu Ke Ding Source Type: research

Small peptides: novel targets for modulating plant-rhizosphere microbe interactions
Trends Microbiol. 2024 Apr 25:S0966-842X(24)00085-4. doi: 10.1016/j.tim.2024.03.011. Online ahead of print.ABSTRACTThe crucial role of rhizosphere microbes in plant growth and their resilience to environmental stresses underscores the intricate communication between microbes and plants. Plants are equipped with a diverse set of signaling molecules that facilitate communication across different biological kingdoms, although our comprehension of these mechanisms is still evolving. Small peptides produced by plants (SPPs) and microbes (SPMs) play a pivotal role in intracellular signaling and are essential in orchestrating var...
Source: Trends in Microbiology - April 26, 2024 Category: Microbiology Authors: Weiyi Tan Hai Nian Lam-Son Phan Tran Jing Jin Tengxiang Lian Source Type: research

Mutation-guided vaccine design: A process for developing boosting immunogens for HIV broadly neutralizing antibody induction
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00126-4. doi: 10.1016/j.chom.2024.04.006. Online ahead of print.ABSTRACTA major goal of HIV-1 vaccine development is the induction of broadly neutralizing antibodies (bnAbs). Although success has been achieved in initiating bnAb B cell lineages, design of boosting immunogens that select for bnAb B cell receptors with improbable mutations required for bnAb affinity maturation remains difficult. Here, we demonstrate a process for designing boosting immunogens for a V3-glycan bnAb B cell lineage. The immunogens induced affinity-matured antibodies by selecting for functional improba...
Source: Cell Host and Microbe - April 26, 2024 Category: Microbiology Authors: Kevin Wiehe Kevin O Saunders Victoria Stalls Derek W Cain Sravani Venkatayogi Joshua S Martin Beem Madison Berry Tyler Evangelous Rory Henderson Bhavna Hora Shi-Mao Xia Chuancang Jiang Amanda Newman Cindy Bowman Xiaozhi Lu Mary E Bryan Joena Bal Aja Sanzo Source Type: research

Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022
CONCLUSION: Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in C. difficile can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.PMID:38670525 | DOI:10.1016/j.anaerobe.2024.102857 (Source: Anaerobe)
Source: Anaerobe - April 26, 2024 Category: Microbiology Authors: Lieke Brouwer Anne Carroll Eleanor McNamara Source Type: research

Antimicrobial resistance profile in Salmonella enterica serovar Choleraesuis isolates from diseased pigs in Taiwan
This study investigated antimicrobial resistance in Salmonella enterica serovar Choleraesuis (S. Choleraesuis) isolates from diseased pigs in Taiwan (2015-2020). Among 272 isolates, florfenicol (96.7%), enrofloxacin (96.3%), doxycycline (91.2%), gentamicin (84.6%), and tiamulin (80.5%) exhibited high resistance. 99.3% of the isolates were resistant to at least one antibiotic, and 97.8% of the isolates were multidrug resistant. This study illustrated that S. Choleraesuis isolates exhibited high resistance to antimicrobials currently used in the Taiwanese swine industry.PMID:38670815 | DOI:10.1016/j.jmii.2024.04.005 (Source:...
Source: Journal of Microbiology, Immunology, and Infection - April 26, 2024 Category: Microbiology Authors: Anh Le Xuan Feng-Yang Hsu Po-Ren Hsueh Wei-Hao Lin Hung-Han Chen Ming-Tang Chiou Chao-Nan Lin Source Type: research

< em > Paraburkholderia translucens < /em > sp. nov. and < em > Paraburkholderia sejongensis < /em > sp. nov., isolated from grassland soil and showing plant growth promoting potential
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006347.ABSTRACTTwo Gram-negative bacterial strains designated MMS20-SJTN17T and MMS20-SJTR3T were isolated from a grassland soil sample, and taxonomically characterized using a polyphasic approach. The 16S rRNA gene sequence analysis indicates that both strains belong to the genus Paraburkholderia of the class Betaproteobacteria, with strain MMS20-SJTN17T being mostly related to Paraburkholderia sprentiae WSM5005T (96.45 % sequence similarity) and strain MMS20-SJTR3T to Paraburkholderia tuberum STM678T (98.59 % sequence similarity). MMS20-SJTN17T could grow at...
Source: International Journal of Systematic and Evolutionary Microbiology - April 26, 2024 Category: Microbiology Authors: Eun Tak Oh Jun Sik Ra Seung Bum Kim Source Type: research

< em > Ruixingdingia sedimenti < /em > gen. nov., sp. nov., a novel taxon within the family < em > Paracoccaceae < /em > isolated from sediment of Qiantang River
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006350.ABSTRACTA Gram-stain-negative bacterium, designated LG-4T, was isolated from sediment of Qiantang River in Zhejiang Province, PR China. Cells were strictly aerobic, non-spore-forming, non-motile and short-rod-shaped (1.0-1.2 µm long and 0.7-0.8 µm wide). Growth occurred at 15-42 °C (optimum, 30 °C), at pH 5.0-9.0 (pH 7.0) and at 0-2.0 % (w/v) NaCl (optimum, 0.5 % NaCl). Strain LG-4T showed 95.75-96.90 % 16S rRNA gene sequence similarity to various type strains of the genera Tabrizicola, Pseudotabrizicola, Phaeovulum, Rhodobacter and Wagnerdoeblera o...
Source: International Journal of Systematic and Evolutionary Microbiology - April 26, 2024 Category: Microbiology Authors: Lu Xu Kexin Wang Luyao Ruan E Chen Songqi Yang Jiguo Qiu Jiandong Jiang Jian He Qirong Shen Source Type: research

Corrigendum to: Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3 β/β-Catenin Signaling Pathway
J Microbiol Biotechnol. 2024 Apr 28;34(4):985-986. doi: 10.4014/jmb.2024.3404.985.NO ABSTRACTPMID:38668686 | DOI:10.4014/jmb.2024.3404.985 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - April 26, 2024 Category: Biotechnology Authors: Yujin Kim Jung Ok Lee Jung Min Lee Mun-Hoe Lee Hyeong-Min Kim Hee-Chul Chung Do-Un Kim Jin-Hee Lee Beom Joon Kim Source Type: research

Efficacy Assessment of the Co-Administration of Vancomycin and Metronidazole in < em > Clostridioides difficile < /em > -Infected Mice Based on Changes in Intestinal Ecology
In this study, a CDI mouse model was constructed to evaluate the therapeutic effects of VAN and MTR alone or in combination. For a perspective on the intestinal ecology, 16S rRNA amplicon sequencing and non-targeted metabolomics techniques were used to investigate changes in the fecal microbiota and metabolome of mice under the co-administration treatment. As a result, the survival rate of mice under co-administration was not dramatically different compared to that of single antibiotics, and the former caused intestinal tissue hyperplasia and edema. Co-administration also significantly enhanced the activity of amino acid m...
Source: Journal of Microbiology and Biotechnology - April 26, 2024 Category: Biotechnology Authors: Saiwei Zhong Jingpeng Yang He Huang Source Type: research

Ginsenoside Rg1 Induces Autophagy in Colorectal Cancer through Inhibition of the Akt/mTOR/p70S6K Pathway
This study aimed to elucidate the anti-colon cancer mechanism of ginsenoside Rg1 in vitro and in vivo. Cell viability rate was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tetrazolium assay. The inhibitory effect of ginsenoside Rg1 against CT26 cell proliferation gradually increased with increasing concentration. The in vivo experiments also demonstrated an antitumor effect. The monodansylcadaverine (MDC), transmission electron microscopy (TEM), and expression of autophagy marker proteins confirmed that ginsenoside Rg1 induced autophagy in vitro. Ginsenoside Rg1 induced autophagy de...
Source: Journal of Microbiology and Biotechnology - April 26, 2024 Category: Biotechnology Authors: Ruiqi Liu Bin Zhang Shuting Zou Li Cui Lin Lin Lingchang Li Source Type: research

Aspergillus Endogenous Endophthalmitis as a Clue to Identifying a Delayed Lumbosacral Osteomyelitis
CONCLUSION: This case highlights the atypical progression of Aspergillus-induced endogenous endophthalmitis to delayed lumbosacral osteomyelitis in an immunocompetent individual. It highlights the crucial role of a meticulous medical history examination and interdisciplinary collaboration in diagnosing and managing diseases, especially in cases with atypical presentations.PMID:38668792 | DOI:10.1080/09273948.2024.2344704 (Source: Ocular Immunology and Inflammation)
Source: Ocular Immunology and Inflammation - April 26, 2024 Category: Allergy & Immunology Authors: Muhammed Altinisik Yeliz Delibay Akgun Mustafa Erdogan Azzam Faiz Mutawakkil Horu Gazi Source Type: research

The comprehensive evaluation of nitrate origin and transformation pathways in the oxic alluvial aquifer in Serbia
Environ Sci Pollut Res Int. 2024 Apr 26. doi: 10.1007/s11356-024-33403-w. Online ahead of print.ABSTRACTAccurate pollution source identification is essential for establishing adequate water management strategies, particularly in groundwater with slow flow and prolonged recharge process allowing long-term pollution retention. An integrated study based on hydrogeochemical, dual isotopic (δ15NNO3 and δ18ONO3), and microbiological approaches (DN, IRB, and SRB BART tests) along with the statistical data processing was conducted to determine nitrate origin and fate in oxic alluvial groundwater source Ključ in Serbia. The find...
Source: Environmental Science and Pollution Research International - April 26, 2024 Category: Environmental Health Authors: Marija Perovi ć Vesna Obradovi ć Vesna Zuber-Radenkovi ć Kay Knoeller David Mitrinovi ć Zoran Čepić Source Type: research