Dissemination, virulence characteristic, antibiotic resistance determinants of emerging linezolid and vancomycin-resistant Enterococcus spp. in fish and crustacean
In conclusion, the emergence of linezolid-resistant -vancomycin-resistant enterococci recovered from Egyptian fish and shrimp, suggests that fish and seafood might participate a fundamental part in the emergence of antimicrobial resistance among humans.PMID:38677237 | DOI:10.1016/j.ijfoodmicro.2024.110711 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - April 27, 2024 Category: Food Science Authors: Sherief M Abdel-Raheem Sherin M Khodier Faisal Almathen Al-Shaimaa T Hanafy Sarah M Abbas Salah Abdulaziz Al-Shami Saad Ibrahim Al-Sultan Ahmed Alfifi Reham M El-Tarabili Source Type: research
The role of rcpA gene in regulating biofilm formation and virulence in Vibrio parahaemolyticus
In conclusion, rcpA plays a role in determining biofilm formation and virulence of V. parahaemolyticus and further research is necessitated to fully understand its function in V. parahaemolyticus.PMID:38677238 | DOI:10.1016/j.ijfoodmicro.2024.110714 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - April 27, 2024 Category: Food Science Authors: Wenxiu Zhu Jiaxiu Liu Yingying Zhang Dongyun Zhao Shugang Li Hanzheng Dou Haisong Wang Xiaodong Xia Source Type: research
Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass
Arch Microbiol. 2024 Apr 27;206(5):236. doi: 10.1007/s00203-024-03974-w.ABSTRACTLignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxiliary enzymes. The De-novo sequencing and analysis of M. verrucosa genome (31.7 Mb) revealed to encode for 7989 putative genes, representing a wide array of CAZymes (366) with a high proportions of auxiliary activity (AA) genes (76). The LC/MS QTOF based secretome analysis of M. verrucosa showe...
Source: Archives of Microbiology - April 27, 2024 Category: Microbiology Authors: Gaurav Sharma Baljit Kaur Varinder Singh Yashika Raheja Marcos Di Falco Adrian Tsang Bhupinder Singh Chadha Source Type: research
Oerskovia flava sp. nov., a deoxynivalenol (DON)-degrading actinomycete isolated from the rhizosphere soil of long-term continuous cropping cucumber
Antonie Van Leeuwenhoek. 2024 Apr 27;117(1):73. doi: 10.1007/s10482-024-01972-y.ABSTRACTThe deoxynivalenol (DON)-degrading bacterium JB1-3-2 T was isolated from a rhizosphere soil sample of cucumber collected from a greenhouse located in Zhenjiang, Eastern China. The JB1-3-2 T strain is a Gram-stain-positive, nonmotile and round actinomycete. Growth was observed at temperatures between 15 and 40 ℃ (optimum, 35 ℃), in the presence of 15% (w/v) NaCl (optimum, 3%), and at pH 3 and 11 (optimum, 7). The major cellular fatty acids identified were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Genome sequencing revealed a genome...
Source: Antonie van Leeuwenhoek - April 27, 2024 Category: Microbiology Authors: Wei Wei Wenjun Guo Biyue Yang Kaili Wang Xinyu Hu Guanya Ji Xiangru Xu Shenkui Liu Lin Zhu Source Type: research
Phage transmission strategies: are phages farming their host?
Curr Opin Microbiol. 2024 Apr 26;79:102481. doi: 10.1016/j.mib.2024.102481. Online ahead of print.ABSTRACTExtensive coevolution has led to utterly intricate interactions between phages and their bacterial hosts. While both the (short-term) intracellular molecular host-subversion mechanisms during a phage infection cycle and the (long-term) mutational arms race between phages and host cells have traditionally received a lot of attention, there has been an underestimating neglect of (mid-term) transmission strategies by which phages manage to cautiously spread throughout their host population. However, recent findings unders...
Source: Current Opinion in Microbiology - April 27, 2024 Category: Microbiology Authors: Yorben Casters Leonard E B äcker Kevin Broux Abram Aertsen Source Type: research
Staphylococcus aureus membrane vesicles: an evolving story
Trends Microbiol. 2024 Apr 26:S0966-842X(24)00088-X. doi: 10.1016/j.tim.2024.04.003. Online ahead of print.ABSTRACTStaphylococcus aureus is an important bacterial pathogen that causes a wide variety of human diseases in community and hospital settings. S. aureus employs a diverse array of virulence factors, both surface-associated and secreted, to promote colonization, infection, and immune evasion. Over the past decade, a growing body of research has shown that S. aureus generates extracellular membrane vesicles (MVs) that package a variety of bacterial components, many of which are virulence factors. In this review, we s...
Source: Trends in Microbiology - April 27, 2024 Category: Microbiology Authors: Xiaogang Wang Jean C Lee Source Type: research
Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A
This study laid a foundation for industrial production and application development of iturin A, and provided the guidance of metabolic engineering breeding for efficient production of other metabolites synthesized by non-ribosomal peptide synthetase. KEY POINTS: • Optimizing 5'-UTR is an effective tactics to regulate synthetase cluster expression. • Blocking 1-DNJ synthesis benefited corn starch utilization and iturin A production. • The iturin A yield attained in this work was the highest yield reported so far.PMID:38676716 | DOI:10.1007/s00253-024-13083-9 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 27, 2024 Category: Microbiology Authors: Menglin She Huijuan Zhou Wanrong Dong Yuxiang Xu Lin Gao Jiaming Gao Yong Yang Zhifan Yang Dongbo Cai Shouwen Chen Source Type: research
The Predictive Factors Associated with In-Hospital Mortality of Melioidosis: A Cohort Study
Conclusions: Our study identified age, respiration rate, abnormal chest X-ray finding, and CO2 levels are predictive factors associated with in-hospital mortality in melioidosis patients. Physicians should be aware of these factors, have access to aggressive treatment options, and closely monitor patients with these risk factors.PMID:38674300 | DOI:10.3390/medicina60040654 (Source: Medicina (Kaunas))
Source: Medicina (Kaunas) - April 27, 2024 Category: Universities & Medical Training Authors: Sunee Chayangsu Chusana Suankratay Apichat Tantraworasin Jiraporn Khorana Source Type: research
Investigation of Cytotoxic and Antileishmanial Activity of Hybrid Silver Nanoparticle Complexes: Potential Drug Candidates against Leishmania Species
In this study, it was aimed to investigate the cytotoxic and in vitro antileishmanial activity of hybrid silver nanoparticle (AgNP) complexes. In this study, three types of nanoparticles (NPs), oxidized amylose-silver (OA-Ag) NPs, oxidized amylose-curcumin (OA-Cur) NPs and oxidized amylose-curcumin-silver (OA-CurAgNP) nanoparticles were synthesized. The cytotoxic activity of the synthesized nanoparticles was determined against L929 mouse fibroblasts and the in vitro antileishmanial activity was determined against Leishmania tropica, Leishmania infantum and Leishmania donovani isolates by the broth microdilution method. It ...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Yener Özel İbrahim Çavuş Umut Yilmaz Feyzullah Tokay Sema Ba ğdat Ahmet Özbilgin Mehmet Ünlü G ülhan Vardar Ünlü Source Type: research
Comparison of Three Different Methods in Investigating the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates
In conclusion, MALDI-TOF MS may not function as a gold standard method like PFGE and MLST for epidemiological analysis in A.baumannii species and the epidemiological typing protocols used for MALDI-TOF MS need to be improved and developed.PMID:38676579 | DOI:10.5578/mb.202498131 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: G ülşen Uluçam Atay G ülçin Bayramoğlu İlknur Tosun Ülkü Ünsal Source Type: research
Silencing of Resistance Mechanism in Vancomycin-Resistance Enterococci Using Antisense RNA of vanA Gene
This study showed that neutralization of the vancomycin resistance gene may provide a lower MIC value in a vancomycin-resistant enterococcus strain and lead to increased susceptibility. This new approach provides a new method for VRE treatment by neutralizing the vancomycin resistance mechanism. The result obtained in this study needs to be supported by in vivo tests.PMID:38676581 | DOI:10.5578/mb.202498191 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Melis Yal çin Melahat Kurtulu ş B ülent Bozdoğan Source Type: research
Molecular Analysis of Antibiotic Resistance in CarbapenemResistant Pseudomonas aeruginosa Isolates Isolated from Clinical Samples
In this study, it was aimed to investigate the molecular epidemiology of 47 carbapenem resistant P.aeruginosa (CRPA) isolates derived from various clinical samples from the Central Laboratory Bacteriology Unit of Kocaeli University Research and Training Hospital between October 2021 and March 2023. The rates of resistance to the antibiotics, some carbapenemase and virulence genes, conjugative resistance plasmids, integron gene cassette contents and the clonal similarity of the isolates were investigated and then epidemiologically evaluated. In the study, identification of the bacterial isolates and their susceptibility to ...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: G ülşah Altan Erva Rakici Osman Birol Özgümüş Source Type: research
Evaluation of Toll-Like Receptor Gene Expressions in Encephalitozoon intestinalis Infection
In conclusion, this study is the f irst report in which the changes in ten different TLR gene expressions were evaluated at different times in MDCK cells stimulated with E.intestinalis and the change in TLR3 gene expression.PMID:38676584 | DOI:10.5578/mb.202498201 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Erdi Uzun Ülfet Çetinkaya Source Type: research
Genetic Diversity of Blastocystis in Diarrheal Cases: Identification of Subtypes and Alleles
In conclusion, our study provides important data on the molecular epidemiological characteristics of the Blastocystis by determining positivity, subtypes and alleles in diarrheal cases. Therefore, within the scope of the one health approach, comprehensive molecular epidemiological studies are required to determine the presence and genotypes of Blastocystis in human, animal and environmental samples.PMID:38676586 | DOI:10.5578/mb.202498207 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Evren Tileklio ğlu Hatice Ertabaklar Source Type: research
Lomentospora prolificans Fungemia in a Patient With T-Cell Large Granular Leukemia: A Rare Pathogen in T ürkiye
In conclusion, although L.prolificans infections are rare, they can be life-threatening, especially in immunosuppressed individuals. Diagnosis and treatment of such infections may be difficult, therefore rapid diagnostic methods and appropriate treatment protocols should be developed. Consideration of infections caused by rare fungal pathogens in patients with risk factors may be critical for patient care. The literature review revealed that the first case of L.prolificans fungemia from Türkiye was reported in 2023. This case presentation represents the second reported case. However, in our case, L.prolificans fungemia oc...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Esat K ıvanç Kaya Mustafa Berkay Ta ştekin Sevtap Arikan Akda ğli Dolunay G ülmez Arzu Topeli Ömrüm Uzun Source Type: research
