Purification and Characterization of a Small Thermostable Protease from < em > Streptomyces < /em > sp. CNXK100
This study focused on the purification and characterization of the thermostable protease obtained from Streptomyces sp. CNXK100. The purified protease exhibited an estimated molecular weight of 27 kDa, with optimal activity at 75°C and pH 8.0. Notably, the enzyme remained active even without any metal ions and fully active in the presence of Na+, K+, Mg2+, and Cu2+metal ions. The kinetic parameters were determined with a KM value of 3.13 mg/ml and a Vmax value of 3.28 × 106 U/mg. Furthermore, the protease has demonstrated notable stability when subjected to a treatment temperature of up to 65°C for 60 minutes, and acros...
Source: Polish Journal of Microbiology - April 28, 2024 Category: Microbiology Authors: Tan Viet Pham Truong Chinh Hua Ngoc An Nguyen Hanh Thi Dieu Nguyen Source Type: research
Short-Chain Carboxylates Facilitate the Counting of Yeasts in Sub-High Temperature Daqu
In this study, short-chain carboxylates (C1-C6) were added to the culture medium at various pH conditions to investigate their effects on the growth of molds and yeasts. The results demonstrated distinct inhibitory effects of the short-chain carboxylates, depending on both pH and concentration. Several tested short-chain carboxylates effectively suppressed mold growth on agar plates while leaving yeast growth unaffected. This suggests a simple and feasible method for enhancing the efficiency of yeast isolation and counting in Daqu. Such an approach is valuable for studying yeasts in diverse and complex habitats.PMID:386784...
Source: Polish Journal of Microbiology - April 28, 2024 Category: Microbiology Authors: Zhiqiang Ren Juan Xie Tuoxian Tang Zhiguo Huang Source Type: research
Insights into the antibacterial effectiveness of linalool against Shigella flexneri on pork surface: Changes in bacterial growth and pork quality
This study aimed to examine the effectiveness of linalool (a natural preservative) against S. flexneri and explore its potential application in contaminated pork. The results showed that linalool was capable of damaging the cell membrane and binding to the DNA of S. flexneri, and inhibiting biofilm formation and disrupting mature biofilms. The antibacterial effectiveness of linalool on the surface of pork was further demonstrated by analyzing the physicochemical properties of the pork (i.e., weight loss rate, pH value, color index, and TVB-N value) and its protein profiles. Linalool did not completely kill S. flexneri in p...
Source: International Journal of Food Microbiology - April 28, 2024 Category: Food Science Authors: Xueying Song Jiamu Kang Xingyan Wei Liu Liu Yongfeng Liu Feng Wang Source Type: research
Current status and new experimental diagnostic methods of invasive fungal infections after hematopoietic stem cell transplantation
Arch Microbiol. 2024 Apr 28;206(5):237. doi: 10.1007/s00203-024-03905-9.ABSTRACTInvasive fungal infections (IFIs) are common and life-threatening complications in post-hematopoietic stem cell transplantation (post-HSCT) recipients, Severe IFIs can lead to systemic infection and organ damage, which results in high mortality in HSCT recipients. With the development of the field of fungal infection diagnosis, more and more advanced non-culture diagnostic tools have been developed, such as glip biosensors, metagenomic next-generation sequencing, Magnetic Nanoparticles and Identified Using SERS via AgNPs+ , and artificial intel...
Source: Archives of Microbiology - April 28, 2024 Category: Microbiology Authors: Zhenhua Tang HaiTao Wang Yuankai Liu Chen Wang Xinye Li Qiong Yang Source Type: research
PII-like signaling proteins: a new paradigm in orchestrating cellular homeostasis
Curr Opin Microbiol. 2024 Apr 27;79:102453. doi: 10.1016/j.mib.2024.102453. Online ahead of print.ABSTRACTMembers of the PII superfamily are versatile, multitasking signaling proteins ubiquitously found in all domains of life. They adeptly monitor and synchronize the cell's carbon, nitrogen, energy, redox, and diurnal states, primarily by binding interdependently to adenyl-nucleotides, including charged nucleotides (ATP, ADP, and AMP) and second messengers such as Cyclic adenosine monophosphate, Cyclic di-adenosine monophosphate, and S-adenosylmethionine-AMP (SAM-AMP). These proteins also undergo a variety of posttranslati...
Source: Current Opinion in Microbiology - April 28, 2024 Category: Microbiology Authors: Khaled A Selim Vikram Alva Source Type: research
T6SS-effector hunters uncover PIX: a novel delivery/marker domain
Trends Microbiol. 2024 Apr 27:S0966-842X(24)00094-5. doi: 10.1016/j.tim.2024.04.009. Online ahead of print.ABSTRACTGram-negative bacteria use the T6SS to eject effectors into prey cells, aided by delivery domains. Recently uncovered by Carobbi et al., a new delivery domain, PIX, allowed the identification of hundreds of new effectors. They are order- and function-specific and exclusively orphan effectors, raising novel questions in the field.PMID:38679505 | DOI:10.1016/j.tim.2024.04.009 (Source: Trends in Microbiology)
Source: Trends in Microbiology - April 28, 2024 Category: Microbiology Authors: Patricia Bernal Source Type: research
The effects of Juniperi fructus essential oil and vacuum packing on the shelf life of rainbow trout fillets during storage at 2 ±1°C
In conclusion, based primarily on sensory, TVB-N and mesophilic bacteria data the shelf-life of fresh rainbow trout was 4 days (non-vacuum packaged), 13 days (vacuum packaged), 19 and 28 days treated with J. fructus oil (0.3 and 0.6%, v/w) under vacuum packaged, respectively. J. fructus essential oil application and vacuum packaging; extended the shelf life of fish fillets by an average of 15 days. The combined use of J. fructus essential oil and packaging techniques could form the basis for new studies.PMID:38678631 | DOI:10.14715/cmb/2024.70.4.5 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 28, 2024 Category: Molecular Biology Authors: Emine Özpolat Source Type: research
Oerskovia flava sp. nov., a deoxynivalenol (DON)-degrading actinomycete isolated from the rhizosphere soil of long-term continuous cropping cucumber
Antonie Van Leeuwenhoek. 2024 Apr 27;117(1):73. doi: 10.1007/s10482-024-01972-y.ABSTRACTThe deoxynivalenol (DON)-degrading bacterium JB1-3-2 T was isolated from a rhizosphere soil sample of cucumber collected from a greenhouse located in Zhenjiang, Eastern China. The JB1-3-2 T strain is a Gram-stain-positive, nonmotile and round actinomycete. Growth was observed at temperatures between 15 and 40 ℃ (optimum, 35 ℃), in the presence of 15% (w/v) NaCl (optimum, 3%), and at pH 3 and 11 (optimum, 7). The major cellular fatty acids identified were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Genome sequencing revealed a genome...
Source: Antonie van Leeuwenhoek - April 27, 2024 Category: Microbiology Authors: Wei Wei Wenjun Guo Biyue Yang Kaili Wang Xinyu Hu Guanya Ji Xiangru Xu Shenkui Liu Lin Zhu Source Type: research
The Predictive Factors Associated with In-Hospital Mortality of Melioidosis: A Cohort Study
Conclusions: Our study identified age, respiration rate, abnormal chest X-ray finding, and CO2 levels are predictive factors associated with in-hospital mortality in melioidosis patients. Physicians should be aware of these factors, have access to aggressive treatment options, and closely monitor patients with these risk factors.PMID:38674300 | PMC:PMC11052379 | DOI:10.3390/medicina60040654 (Source: Medicina (Kaunas))
Source: Medicina (Kaunas) - April 27, 2024 Category: Universities & Medical Training Authors: Sunee Chayangsu Chusana Suankratay Apichat Tantraworasin Jiraporn Khorana Source Type: research
Lomentospora prolificans Fungemia in a Patient With T-Cell Large Granular Leukemia: A Rare Pathogen in T ürkiye
In conclusion, although L.prolificans infections are rare, they can be life-threatening, especially in immunosuppressed individuals. Diagnosis and treatment of such infections may be difficult, therefore rapid diagnostic methods and appropriate treatment protocols should be developed. Consideration of infections caused by rare fungal pathogens in patients with risk factors may be critical for patient care. The literature review revealed that the first case of L.prolificans fungemia from Türkiye was reported in 2023. This case presentation represents the second reported case. However, in our case, L.prolificans fungemia oc...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Esat K ıvanç Kaya Mustafa Berkay Ta ştekin Sevtap Arikan Akda ğli Dolunay G ülmez Arzu Topeli Ömrüm Uzun Source Type: research
Genetic Diversity of Blastocystis in Diarrheal Cases: Identification of Subtypes and Alleles
In conclusion, our study provides important data on the molecular epidemiological characteristics of the Blastocystis by determining positivity, subtypes and alleles in diarrheal cases. Therefore, within the scope of the one health approach, comprehensive molecular epidemiological studies are required to determine the presence and genotypes of Blastocystis in human, animal and environmental samples.PMID:38676586 | DOI:10.5578/mb.202498207 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Evren Tileklio ğlu Hatice Ertabaklar Source Type: research
Investigation of Cytotoxic and Antileishmanial Activity of Hybrid Silver Nanoparticle Complexes: Potential Drug Candidates against Leishmania Species
In this study, it was aimed to investigate the cytotoxic and in vitro antileishmanial activity of hybrid silver nanoparticle (AgNP) complexes. In this study, three types of nanoparticles (NPs), oxidized amylose-silver (OA-Ag) NPs, oxidized amylose-curcumin (OA-Cur) NPs and oxidized amylose-curcumin-silver (OA-CurAgNP) nanoparticles were synthesized. The cytotoxic activity of the synthesized nanoparticles was determined against L929 mouse fibroblasts and the in vitro antileishmanial activity was determined against Leishmania tropica, Leishmania infantum and Leishmania donovani isolates by the broth microdilution method. It ...
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Yener Özel İbrahim Çavuş Umut Yilmaz Feyzullah Tokay Sema Ba ğdat Ahmet Özbilgin Mehmet Ünlü G ülhan Vardar Ünlü Source Type: research
Evaluation of Toll-Like Receptor Gene Expressions in Encephalitozoon intestinalis Infection
In conclusion, this study is the f irst report in which the changes in ten different TLR gene expressions were evaluated at different times in MDCK cells stimulated with E.intestinalis and the change in TLR3 gene expression.PMID:38676584 | DOI:10.5578/mb.202498201 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Erdi Uzun Ülfet Çetinkaya Source Type: research
Silencing of Resistance Mechanism in Vancomycin-Resistance Enterococci Using Antisense RNA of vanA Gene
This study showed that neutralization of the vancomycin resistance gene may provide a lower MIC value in a vancomycin-resistant enterococcus strain and lead to increased susceptibility. This new approach provides a new method for VRE treatment by neutralizing the vancomycin resistance mechanism. The result obtained in this study needs to be supported by in vivo tests.PMID:38676581 | DOI:10.5578/mb.202498191 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: Melis Yal çin Melahat Kurtulu ş B ülent Bozdoğan Source Type: research
Comparison of Three Different Methods in Investigating the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates
In conclusion, MALDI-TOF MS may not function as a gold standard method like PFGE and MLST for epidemiological analysis in A.baumannii species and the epidemiological typing protocols used for MALDI-TOF MS need to be improved and developed.PMID:38676579 | DOI:10.5578/mb.202498131 (Source: Mikrobiyoloji Bulteni)
Source: Mikrobiyoloji Bulteni - April 27, 2024 Category: Microbiology Authors: G ülşen Uluçam Atay G ülçin Bayramoğlu İlknur Tosun Ülkü Ünsal Source Type: research
