Silent Genes: Antimicrobial Resistance and Antibiotic Production
Pol J Microbiol. 2021 Dec;70(4):421-429. doi: 10.33073/pjm-2021-040. Epub 2021 Dec 23.ABSTRACTSilent genes are DNA sequences that are generally not expressed or expressed at a very low level. These genes become active as a result of mutation, recombination, or insertion. Silent genes can also be activated in laboratory conditions using pleiotropic, targeted genome-wide, or biosynthetic gene cluster approaches. Like every other gene, silent genes can spread through horizontal gene transfer. Most studies have focused on strains with phenotypic resistance, which is the most common subject. However, to fully understand the mec...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Monika Stasiak El żbieta Maćkiw Joanna Kowalska Katarzyna Kucharek Jacek Postupolski Source Type: research

Can < em > Lactobacillus < /em > spp. Be a Factor Reducing the Risk of Miscarriage?
Pol J Microbiol. 2021 Dec;70(4):431-446. doi: 10.33073/pjm-2021-043. Epub 2021 Dec 23.ABSTRACTPregnancy loss is a common obstetric problem. Significant causes of miscarriage include genetic and epigenetic disorders of the embryo, immunological and endocrine factors, uterine malformations, improper embryo selection, and lifestyle. Perhaps a hitherto underappreciated cause of miscarriage may be an abnormal microbiota composition of the female reproductive system. Lactobacillus spp. is the most common bacteria within the reproductive tract. However, the protective role of Lactobacilli in the vagina has been well described in ...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Aneta Kiecka Barbara Macura Marian Szczepanik Source Type: research

Chemical Components of Aqueous Extracts of < em > Melia azedarach < /em > Fruits and Their Effects on The Transcriptome of < em > Staphylococcus aureus < /em >
Pol J Microbiol. 2021 Dec;70(4):447-459. doi: 10.33073/pjm-2021-041. Epub 2021 Dec 23.ABSTRACTStaphylococcus aureus is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of Melia azedarach fruits inhibit the planktonic growth and initial biofilm formation of S. aureus in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after S. aureus exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The ma...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Hong Peng Ying-Si Wang Jie Wang Su-Juan Li Ting-Li Sun Tong Liu Qing-Shan Shi Gang Zhou Xiao-Bao Xie Source Type: research

QuantiFERON-TB Gold Plus Test in Diagnostics of Latent Tuberculosis Infection in Children Aged 1-14 in a Country with a Low Tuberculosis Incidence
The objective of this study was to evaluate the QFT-Plus affectivity as a diagnostic test in the absence of a gold standard (GS) test for the diagnosis of LTBI. The children were diagnosed with QFT-Plus, TST, and culture of TB. The QFT-Plus results were analyzed depending on the children's age, TST size, and type. In children aged 1-4, the positive predictive value of QFT-Plus was 1, the negative predictive value was 0.94, QFT-Plus sensitivity was 75%, and specificity was 100%. It was observed that in children aged 5-14 years, the level of agreement decreased to the substantial, i.e., 87.2%. Moreover, the negative predicti...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Dagmara Borkowska-Tatar Maria Krasi ńska Ewa Augustynowicz-Kope ć Source Type: research

Histopathological Analysis of < em > Acinetobacter baumannii < /em > Lung Infection in a Mouse Model
In this study, we analyzed the histopathological characteristics of lung infection with two strains of A. baumannii (ATCC 19606 and the clinical isolate TK1090) and Pseudomonas aeruginosa PAO-1 in C3H/HeN mice to evaluate the virulence of A. baumannii. Survival was evaluated over 14 days. At 1, 2, 5, or 14 days postinfection, mice of C3H/HeN were sacrificed, and histopathological analysis of lung specimens was also performed. Histopathological changes and accumulation of neutrophils and macrophages in the lungs after infection with A. baumannii and P. aeruginosa were analyzed. Following intratracheal inoculation, the letha...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Shigeru Tansho-Nagakawa Yoshinori Sato Tsuneyuki Ubagai Takane Kikuchi-Ueda G O Kamoshida Satoshi Nishida Yasuo Ono Source Type: research

A Novel LysR Family Factor STM0859 is Associated with The Responses of < em > Salmonella < /em > Typhimurium to Environmental Stress and Biofilm Formation
This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.PMID:35003279 | PMC:PMC8702606 | DOI:10.33073/pjm-2021-045 (Source: Polish Journal of Microbiology)
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Zhongmei Ma N A Li Chengcheng Ning Yucheng Liu Yun Guo Chunhui Ji Xiaozhen Zhu Qingling Meng Xianzhu Xia Xingxing Zhang Xuepeng Cai Kuojun Cai Qiao Jun Source Type: research

Identification of Genetic Variants of Human Papillomavirus in a Group of Mexican HIV/AIDS Patients and Their Possible Association with Cervical Cancer
The objective of the study was to identify the presence of genetic variants of HPV in a group of HIV-positive women and their possible association with cervical cancer. Cervical samples were taken from HIV-positive patients for cytological analysis to identify the HPV genotype by polymerase chain reaction (PCR) and sequencing. The most prevalent L1 capsid protein mutations in the HPV genotype were analyzed in silico. Various types of HPV were identified, both high-risk (HR) and low-risk (LR). The most prevalent genotype was HPV51. Analysis of the L1 gene sequences of HPV51 isolates showed nucleotide variations. Of the samp...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Felipe Ortiz-Guti érrez Lilia S ánchez-Minutti Jos é F Martínez-Herrera Indiana D Torres-Escobar Elias B Pezzat-Said Luis M árquez-Domínguez Amado I Grandes-Blanco Source Type: research

Lipoic Acid Does Not Affect The Growth of < em > Mycoplasma hominis < /em > Cells < em > In Vitro < /em >
Pol J Microbiol. 2021 Dec;70(4):521-526. doi: 10.33073/pjm-2021-050. Epub 2021 Dec 23.ABSTRACTMycoplasma hominis is associated with various infections, for which the treatment can be complex. Lipoic acid (LA) plays a role as a cofactor in eukaryotes, most Bacteria, and some Archea. Research of recent years has increasingly pointed to the therapeutic properties of exogenously supplemented LA. The present study was conducted on 40 strains of M. hominis cultured with the following LA concentrations: 1,200 μg/ml, 120 μg/ml, and 12 μg/ml. The bacterial colonies of each strain were counted and expressed as the number of colon...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Ma łgorzata Biernat-Sudolska Danuta Rojek-Zakrzewska Paulina Gajda Anna Bilska-Wilkosz Source Type: research

Silent Genes: Antimicrobial Resistance and Antibiotic Production
Pol J Microbiol. 2021 Dec;70(4):421-429. doi: 10.33073/pjm-2021-040. Epub 2021 Dec 23.ABSTRACTSilent genes are DNA sequences that are generally not expressed or expressed at a very low level. These genes become active as a result of mutation, recombination, or insertion. Silent genes can also be activated in laboratory conditions using pleiotropic, targeted genome-wide, or biosynthetic gene cluster approaches. Like every other gene, silent genes can spread through horizontal gene transfer. Most studies have focused on strains with phenotypic resistance, which is the most common subject. However, to fully understand the mec...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Monika Stasiak El żbieta Maćkiw Joanna Kowalska Katarzyna Kucharek Jacek Postupolski Source Type: research

Can < em > Lactobacillus < /em > spp. Be a Factor Reducing the Risk of Miscarriage?
Pol J Microbiol. 2021 Dec;70(4):431-446. doi: 10.33073/pjm-2021-043. Epub 2021 Dec 23.ABSTRACTPregnancy loss is a common obstetric problem. Significant causes of miscarriage include genetic and epigenetic disorders of the embryo, immunological and endocrine factors, uterine malformations, improper embryo selection, and lifestyle. Perhaps a hitherto underappreciated cause of miscarriage may be an abnormal microbiota composition of the female reproductive system. Lactobacillus spp. is the most common bacteria within the reproductive tract. However, the protective role of Lactobacilli in the vagina has been well described in ...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Aneta Kiecka Barbara Macura Marian Szczepanik Source Type: research

Chemical Components of Aqueous Extracts of < em > Melia azedarach < /em > Fruits and Their Effects on The Transcriptome of < em > Staphylococcus aureus < /em >
Pol J Microbiol. 2021 Dec;70(4):447-459. doi: 10.33073/pjm-2021-041. Epub 2021 Dec 23.ABSTRACTStaphylococcus aureus is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of Melia azedarach fruits inhibit the planktonic growth and initial biofilm formation of S. aureus in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after S. aureus exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The ma...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Hong Peng Ying-Si Wang Jie Wang Su-Juan Li Ting-Li Sun Tong Liu Qing-Shan Shi Gang Zhou Xiao-Bao Xie Source Type: research

QuantiFERON-TB Gold Plus Test in Diagnostics of Latent Tuberculosis Infection in Children Aged 1-14 in a Country with a Low Tuberculosis Incidence
The objective of this study was to evaluate the QFT-Plus affectivity as a diagnostic test in the absence of a gold standard (GS) test for the diagnosis of LTBI. The children were diagnosed with QFT-Plus, TST, and culture of TB. The QFT-Plus results were analyzed depending on the children's age, TST size, and type. In children aged 1-4, the positive predictive value of QFT-Plus was 1, the negative predictive value was 0.94, QFT-Plus sensitivity was 75%, and specificity was 100%. It was observed that in children aged 5-14 years, the level of agreement decreased to the substantial, i.e., 87.2%. Moreover, the negative predicti...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Dagmara Borkowska-Tatar Maria Krasi ńska Ewa Augustynowicz-Kope ć Source Type: research

Histopathological Analysis of < em > Acinetobacter baumannii < /em > Lung Infection in a Mouse Model
In this study, we analyzed the histopathological characteristics of lung infection with two strains of A. baumannii (ATCC 19606 and the clinical isolate TK1090) and Pseudomonas aeruginosa PAO-1 in C3H/HeN mice to evaluate the virulence of A. baumannii. Survival was evaluated over 14 days. At 1, 2, 5, or 14 days postinfection, mice of C3H/HeN were sacrificed, and histopathological analysis of lung specimens was also performed. Histopathological changes and accumulation of neutrophils and macrophages in the lungs after infection with A. baumannii and P. aeruginosa were analyzed. Following intratracheal inoculation, the letha...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Shigeru Tansho-Nagakawa Yoshinori Sato Tsuneyuki Ubagai Takane Kikuchi-Ueda G O Kamoshida Satoshi Nishida Yasuo Ono Source Type: research

A Novel LysR Family Factor STM0859 is Associated with The Responses of < em > Salmonella < /em > Typhimurium to Environmental Stress and Biofilm Formation
This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.PMID:35003279 | PMC:PMC8702606 | DOI:10.33073/pjm-2021-045 (Source: Polish Journal of Microbiology)
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Zhongmei Ma N A Li Chengcheng Ning Yucheng Liu Yun Guo Chunhui Ji Xiaozhen Zhu Qingling Meng Xianzhu Xia Xingxing Zhang Xuepeng Cai Kuojun Cai Qiao Jun Source Type: research

Identification of Genetic Variants of Human Papillomavirus in a Group of Mexican HIV/AIDS Patients and Their Possible Association with Cervical Cancer
The objective of the study was to identify the presence of genetic variants of HPV in a group of HIV-positive women and their possible association with cervical cancer. Cervical samples were taken from HIV-positive patients for cytological analysis to identify the HPV genotype by polymerase chain reaction (PCR) and sequencing. The most prevalent L1 capsid protein mutations in the HPV genotype were analyzed in silico. Various types of HPV were identified, both high-risk (HR) and low-risk (LR). The most prevalent genotype was HPV51. Analysis of the L1 gene sequences of HPV51 isolates showed nucleotide variations. Of the samp...
Source: Polish Journal of Microbiology - January 10, 2022 Category: Microbiology Authors: Felipe Ortiz-Guti érrez Lilia S ánchez-Minutti Jos é F Martínez-Herrera Indiana D Torres-Escobar Elias B Pezzat-Said Luis M árquez-Domínguez Amado I Grandes-Blanco Source Type: research